Southeastern Virginia Research Articles

Limenitis a. arthemis (White Admiral) in coastal southeastern Virginia

Limenitis a. arthemis (White Admiral) in coastal southeastern Virginia

Host Associations of Culex pipiens: A Two-Year Analysis of Bloodmeal Sources and Implications for Arboviral Transmission in Southeastern Virginia.

Understanding vector-host interactions is crucial for evaluating the role of mosquito species in enzootic cycling and epidemic/epizootic transmission of arboviruses, as well as assessing vertebrate host contributions to maintenance and amplification in different virus foci. To investigate blood-feeding pattern of Culex pipiens, engorged mosquitoes were collected on a weekly basis at 50 sites throughout Suffolk, Virginia, using Centers for Disease Control and Prevention miniature light traps, BG-Sentinel traps, and modified Reiter gravid traps. Vertebrate hosts of mosquitoes were identified by amplifying and sequencing portions of the mitochondrial cytochrome b gene. Of 281 Cx. pipiens bloodmeals successfully identified to species, 255 (90.7%) contained solely avian blood, 13 (4.6%) mammalian, 1 (0.4%) reptilian, and 12 (4.3%) both avian and mammalian blood. Nineteen avian species were identified as hosts for Cx. pipiens with American robin (n = 141, 55.3% of avian hosts) and northern cardinal (n = 57, 22.4%) as the most common hosts. More American robin feedings took place in areas of higher development. Three mammalian species were also identified as hosts for Cx. pipiens with Virginia opossum and domestic cat as the most common hosts in this class (each n = 6, 46.2% of mammalian hosts). There was no significant seasonal difference in the proportion of bloodmeals obtained from avian hosts, but there was a decrease in the proportion of bloodmeals from mammalian hosts from spring to fall. One engorged specimen of Cx. pipiens with Virginia opossum-derived bloodmeal tested positive for West Nile virus (WNV), and another with black-and-white warbler-derived bloodmeal tested positive for eastern equine encephalitis virus. Our findings, in conjunction with the results of vector competence studies and virus isolation from field-collected mosquitoes, lend additional support that Cx. pipiens serves as the principal enzootic vector and potential epizootic/epidemic vector of WNV in southeastern Virginia.

Granular activated carbon‐based treatment and mobility of per‐ and polyfluoroalkyl substances in potable reuse for aquifer recharge

AbstractHampton Roads Sanitation District recently initiated an indirect potable reuse project to recharge the Potomac Aquifer in southeastern Virginia, USA. Source control and granular activated carbon (GAC) treatment are employed to minimize per‐ and polyfluoroalkyl substances (PFAS) content in this water. Final purified water maintained low long‐chain perfluoroalkyl acids (PFAAs; <8 ppt sum of PFOA, PFNA, PFDA, PFHxS, PFOS) and moderately low short‐chain PFAAs (<118 ppt of sum PFBA, PFPeA, PFHxA, PFHpA, PFBS). High mobility of low concentrations of five PFAS (PFBS, PFPeA, PFHxA, PFHpA, PFOA) in the Potomac Aquifer was observed. Despite low concentrations relative to current guidelines, PFAS breakthrough and mobility support strict source control and the use of surrogates like total organic carbon to guide GAC operation at the installation.Article Impact StatementSource control and granular activated carbon operation targeting 4 mg/L total organic carbon in effluent maintained low per‐ and polyfluoroalkyl substances concentrations in indirect potable reuse water.

Soil type and water level treatments influence peatland CO2 efflux in simulated restoration

Peatlands are characterized by accumulation of organic matter primarily in response to soil anoxia and soil saturation. In the Cavalier Wildlife Management Area (CWMA) of southeastern Virginia, weirs were installed within ditches in an effort to restore peatlands. The purpose of this research was to determine the water levels that restore soil respiration functions and encourage carbon dioxide (CO2) sequestration. Core microcosms of two soil types (histosol and ultisol) were collected and established in the laboratory with differing water level treatments with simulated depth to water table. Incubations continued for 1 month and CO2 emission rates of the soil cores were measured twice per week using a portable photosynthesis instrument. Results from a mixed linear model and Tukey post-hoc tests demonstrated water level treatments were inversely correlated to CO2 emissions regardless of soil type and that the ultisol soil type exhibited lower percent volumetric water content and higher CO2 emissions overall. This suggests that higher water tables suppress CO2 emissions and can increase carbon sequestration, and that natural resource managers should evaluate soil types when establishing restoration and carbon sequestration goals, as soil types have differing responses to hydrologic restoration.

First Record of Panoquina panoquin (Salt Marsh Skipper) Ovipositing on Spartina alterniflora (Smooth Cordgrass)

Panoquina panoquin (Salt Marsh Skipper) is an understudied butterfly that resides primarily in coastal salt marshes throughout the southern Atlantic and Gulf of Mexico. Based on our review of online databases and published literature, this species may use several different host plants, though Distichlis spicata (Saltgrass) is most commonly cited. While surveying salt marsh butterfly communities in southeastern Virginia in 2019, we observed 25 oviposition events from female Salt Marsh Skippers, all of which laid their eggs exclusively on Spartina alterniflora (Smooth Cordgrass). This is the first conclusive record of the Salt Marsh Skipper using this species of grass for egg laying. Our observations broaden the understanding of Salt Marsh Skipper habitat requirements and therefore inform conservation efforts for this species in the face of degradation of salt marshes throughout its range.

Passerine birds as hosts for Ixodes ticks infected with Borrelia burgdorferi sensu stricto in southeastern Virginia

The ecology of vector-borne diseases in a region can be attributed to vector-host interactions. In the United States, tick-borne pathogens are the cause of the highest number of reported vector-borne diseases. In the mid-Atlantic region of the eastern United States, tick-borne diseases such as Lyme disease, have increased in incidence, with tick-host-pathogen interactions considered a contributing factor to this increase. Ticks become infected with pathogens after taking a blood meal from a systemically infected host or through a localized infection while co-feeding on a host with other infected ticks. The host not only plays a role in pathogen acquisition by the tick, but can also facilitate dispersal of the tick locally within a region or over greater distances into new geographical ranges outside of their historical distributional range. In this study conducted in southeastern Virginia (USA), we examined the interaction between both resident and migratory bird species and Ixodes ticks, the primary vectors of Borrelia burgdorferi sensu stricto (s.s.) the main causative agent of Lyme disease on the East coast of the United States. Over a two-year period (2012–2014), 1879 passerine birds were surveyed, with 255 Ixodes ticks tested for the presence of Borrelia spp. Eighty passerine birds (4.3 %) representing 17 bird species were parasitized by at least one Ixodes tick, but only three bird species were parasitized by Ixodes ticks that tested positive for B. burgdorferi s.s. Twenty Ixodes ticks (7.8 %) tested positive for B. burgdorferi s.s. with nearly all collected from resident bird species including the Carolina wren (Thryothorus ludovicianus) and brown thrasher (Toxostoma rufum). Given that millions of birds pass through southeastern Virginia during migration, even with the low number of Ixodes ticks parasitizing passerine birds and the low prevalence of B. burgdorferi s.s. found within Ixodes ticks collected, the sheer volume of passerine birds suggests they may play a role in the maintenance and dispersal of B. burgdorferi s.s. in southeastern Virginia.

Review: Gerrymanders: How Redistricting Has Protected Slavery, White Supremacy, and Partisan Minorities in Virginia, by Brent Tarter

Review: <i>Gerrymanders: How Redistricting Has Protected Slavery, White Supremacy, and Partisan Minorities in Virginia</i>, by Brent Tarter

A Comparison of Tick Collection Materials and Methods in Southeastern Virginia.

In field studies of tick ecology, observed patterns may be biased by sampling methods. Results can vary by species, life stage, and habitat, and understanding these biases will improve comparisons of data across studies as well as assessment of human disease risk. A direct comparison of flagging versus dragging was conducted in southeastern Virginia. Transects were surveyed over a 6-wk period to identify differences in species and life stage collected, as well as differences between corduroy and denim material and inspection method for drags. Flagging collected more Ixodes affinis Neumann (Acari: Ixodidae) adults and Amblyomma americanum L. (Acari: Ixodidae) adults than dragging. Ground inspection was more efficient than tree inspection for collection of I. affinis adults, with no significant difference in inspection method for any other species or life stage. Corduroy was found to be more effective than denim in collecting nymphal A. americanum, although this may be an artifact of three large samples for corduroy collection of these ticks. There was no significant difference in Ixodes scapularis Say (Acari: Ixodidae) collection in any comparison. Dragging, tree inspection, and denim were not found to be more efficient in any scenario. This is the first comparison of flagging and dragging conducted in the southeastern United States. The community composition of ticks in this region greatly differs from regions where studies of these commonly used sampling techniques have been conducted. As the distributions of ticks continue to change over time, it will be important to evaluate best practices annually.

Collection system investigation microbial source tracking (CSI-MST): Applying molecular markers to identify sewer infrastructure failures

Collection System Investigation Microbial Source Tracking (CSI-MST) is a novel, sensitive approach for identifying sewer infrastructure deficiencies using molecular markers. This method requires both a detailed understanding of collection and conveyance system infrastructure and quickly turned around molecular data to advise an adaptive, targeted in-pipe approach to detect deficiencies. Here we explain the CSI-MST approach and provide several case study examples of how this approach can be adapted to different scale watersheds to identify potential sewer infrastructure issues. This approach has been used to locate and confirm the remediation of numerous needed infrastructure repairs in the southeastern Virginia region. The selected case studies presented here serve as a proof of concept—this methodology can be adopted by other utilities and municipalities to address necessary wastewater infrastructure repairs in different regions.

Survey of Rickettsia parkeri and Amblyomma maculatum associated with small mammals in southeastern Virginia

Small mammals are often parasitized by the immature stages of hard-bodied ticks (family Ixodidae) and may serve as reservoir hosts of tick-borne pathogens. Amblyomma maculatum, the Gulf Coast tick, is the primary vector of Rickettsia parkeri, the causative agent of R. parkeri rickettsiosis. This hard-bodied tick species is expanding its historical range from the Gulf Coast of the U.S. up the Mid-Atlantic coast. In Mid-Atlantic states, such as Virginia, R. parkeri prevalence is higher in these ticks than those found in its historical range. This high prevalence may be explained in part by small mammal populations. In this study, small mammals were trapped and checked for the presence of immature A. maculatum. The ticks as well as tissue samples from these mammals were tested for the presence of R. parkeri. This study found six rodent species acting as hosts to immature A. maculatum and three species that may play a role in the enzootic cycle of R. parkeri in Virginia.

COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology

Wastewater-based epidemiology (WBE) has been used to analyze markers in wastewater treatment plant (WWTP) influent to characterize emerging chemicals, drug use patterns, or disease spread within communities. This approach can be particularly helpful in understanding outbreaks of disease like the novel Coronavirus disease-19 (COVID-19) when combined with clinical datasets. In this study, three RT-ddPCR assays (N1, N2, N3) were used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in weekly samples from nine WWTPs in southeastern Virginia. In the first several weeks of sampling, SARS-CoV-2 detections were sporadic. Frequency of detections and overall concentrations of RNA within samples increased from mid March into late July. During the twenty-one week study, SARS-CoV-2 concentrations ranged from 101 to 104 copies 100 mL−1 in samples where viral RNA was detected. Fluctuations in population normalized loading rates in several of the WWTP service areas agreed with known outbreaks during the study. Here we propose several ways that data can be presented spatially and temporally to be of greatest use to public health officials. As the COVID-19 pandemic wanes, it is likely that communities will see increased incidence of small, localized outbreaks. In these instances, WBE could be used as a pre-screening tool to better target clinical testing needs in communities with limited resources.

Local and regional temporal trends (2013\u20132019) of canine Ehrlichia spp. seroprevalence in the USA

BackgroundIn the USA, there are several Ehrlichia spp. of concern including Ehrlichia canis, Ehrlichia ewingii, Ehrlichia chaffeensis, Ehrlichia muris eauclarensis, and “Panola Mountain Ehrlichia”. Of these, E. canis is considered the most clinically relevant for domestic dogs, with infection capable of causing acute, subclinical, and chronic stages of disease. Changes in climate, land use, habitats, and wildlife reservoir populations, and increasing contact between both human and dog populations with natural areas have resulted in the increased risk of vector-borne disease throughout the world.MethodsA Bayesian spatio-temporal binomial regression model was applied to serological test results collected from veterinarians throughout the contiguous USA between January 2013 and November 2019. The model was used to quantify both regional and local temporal trends of canine Ehrlichia spp. seroprevalence and identify areas that experienced significant increases in seroprevalence.ResultsRegionally, increasing seroprevalence occurred within several states throughout the central and southeastern states, including Missouri, Arkansas, Mississippi, Alabama, Virginia, North Carolina, Georgia and Texas. The underlying local trends revealed increasing seroprevalence at a finer scale. Clusters of locally increasing seroprevalence were seen from the western Appalachian region into the southern Midwest, along the Atlantic coast in New England, parts of Florida, Illinois, Wisconsin and Minnesota, and in a couple areas of the Mountain region. Clusters of locally decreasing seroprevalence were seen throughout the USA including New York and the mid-Atlantic states, Texas, the Midwest, and California.ConclusionsCanine Ehrlichia spp. seroprevalence is increasing in both endemic and non-endemic areas of the USA. The findings from this study indicate that dogs across a wide area of the USA are at risk of exposure and these results should provide veterinarians and pet owners with the information they need to make informed decisions about prevention of tick exposure.

Assessing Instagram Use Across Cultures: A Confirmatory Factor Analysis

The Instagram and Well-Being Questionnaire (IWBQ) is a 19-item self-report inventory that assesses Instagram and social media use. Item 14 of the measure contains 40 subitems specifically assessing reasons for use. Prior research in Australia regarding Item 14 specifically revealed a three-factor structure: (a) sense of belonging, (b) self-expression, and (c) documentation/curation. This study sought to assess the goodness of fit for this factor structure in a sample of undergraduate college students from the United States. Data were collected from 735 students at two public universities in Southeastern Virginia; 520 of whom were retained for the analyses. Confirmatory factor analysis using the mean and variance-adjusted weighted least squares method was conducted using Mplus 8.1 to assess goodness of model fit. The three-factor model failed to meet criteria for exact or approximate model fit: χ2(87) = 1542.82, p < 0.001; root mean square error of approximation = 0.19, 95 percent confidence interval = 0.18-0.20; comparative fit index = 0.77; Tucker/Lewis index = 0.73. Follow-up exploratory analyses were conducted to assess the model fit after partitioning the sample based on racial identity (African American and Caucasian) to explore this potential sociocultural confound. In both cases, the model did not demonstrate goodness of fit. These results indicate that global crosscultural differences may impact the reasons why individuals use Instagram, meaning the same model may not maintain reliable fit across cultures.

If You Can Program, You Can Write: Learning Introductory Programming Across Literacy Levels

Aim/Purpose: This paper presents findings on a curricular intervention aimed at integrating computer programming with reading and writing in early elementary school. The purpose of this research was to explore the relation between students’ varying literacy levels and their level of success in mastering an introductory programming language. Methodology: This curricular intervention study was implemented in a single school district in southeastern Virginia. Of the district’s 33 elementary schools, eight schools received an external grant from the U.S. Department of Defense to introduce computer science in early elementary education. Standardized literacy test scores were correlated with internally developed, and age appropriate programming assessment scores from N = 132 second grade students. Contribution: This study is the first of its kind to look at how students at varying literacy levels succeed in mastering an introductory programming language when introduced through a literacy lens. Findings: The findings indicated that there was strong evidence for a weak, positive correlation between students’ literacy levels, as determined by the PALS assessment, and their programming mastery, as determined by the curricular programming assessments. The positive correlation suggests that there may indeed be underlying constructs that overlap between literacy and programming. Recommendations for Practitioners: Consider integrating computer programming as a foundational component of the literacy curriculum, especially in the early grades, where the two skill sets can mutually support one another. Recommendation for Researchers: Additional research is necessary, using a variety of literacy and programming measures, to continue to understand the relationship between emerging literacy and emerging computer programming skills. Impact on Society: Reimagining computer programming as a language has significant implications for how we teach programming in schools and how students then use programming out in the workforce. Future Research: Future work will repeat this curricular intervention with younger students: the district’s first grade and kindergarten classrooms. Introducing programming through the Coding as Literacy (CAL) approach even earlier in students’ literacy trajectories, we believe, will allow the positive impact of programming knowledge to influence students’ literacy development. In this next phase of our research agenda, we will collect pre and post literacy scores, both standardized and internally developed, to see the myriad ways that programming knowledge impacts literacy.

Hiring Strategies for Small Business Owner to Recruit Veterans (A Multiple Case Study)

Grounded in recruitment theory, the purpose of this qualitative multiple case study was to explore the strategies used by leaders of small businesses to recruit, hire, and retain qualified veterans. The targeted population consisted of three leaders of small businesses in the southeastern Virginia area who have used successful strategies to recruit, hire, and retain veterans. Data were collected using semistructured interviews and document review. Data were analyzed using thematic analysis, and 4 themes emerged: social media advertisement, local networking with military facilities, workshop and job description, and resume review and effective communication. The finding of this study included that participants advertised job vacancies through social media, military post advertisements, human resource boards, and small business administration. Each participant agreed that the labor force hiring process consists of competition with other civilian entities. The implications for positive social change in organizations include influencing owners of small businesses with knowledge of potential growth in local economies and supporting military veterans.

EMERGING MODELS OF CARE: THE IMPACT OF PHARMACIST-LED MEDICATION MANAGEMENT IN A TRANSITIONAL CARE PROGRAM

Transitional care programs have emerged as successful models of care in which to reduce cost and improve health outcomes. However, few transitional care models have directly incorporated the expertise of the pharmacist as an integral member of the care coordination team. Therein lies an inherent limitation of many community-based transitional care programs, the underutilization of pharmacist during all stages of the care transition process. In 2013, the Hampton Roads Care Transitions Project (HRCTP), a partnership between Senior Services of Southeastern Virginia Area Agency on Aging in Norfolk, VA and Hampton University School of Pharmacy, was established. The goal of the HRCTP is to provide medication management services to reduce preventable hospital readmissions for adults 60 years of age and older with targeted diagnoses. Pharmacists work in collaboration with social workers who act as HRCTP care transition coaches. Between May 2017- October 2018, 678 patients were enrolled in the HRCTP. The hospital readmission rate among patients with targeted diagnoses was reduced by 55.3% with an absolute percentage point reduction of 9.9% and estimated savings amount per avoided readmission of $14,400. Patients who participated in the HRCTP showed a 14% increase in the Patient Activation Assessment indicating an improvement in self-managing efficacy. 93% of patients/caregivers indicated they felt more confident in their ability to manage their health, and 91% expressed satisfaction with the program. The program has proven effective in assisting seniors to remain in their home, reducing hospitalizations, promoting health, increasing patient satisfaction, and reducing healthcare cost.

Habitat amount, quality, and fragmentation associated with prevalence of the tick-borne pathogen Ehrlichia chaffeensis and occupancy dynamics of its vector, Amblyomma americanum

Tick-borne diseases are becoming increasingly prevalent world-wide. This is likely due in part to land-cover change, particularly forest fragmentation, but this evidence is largely limited to Lyme disease. It is unknown whether this is generalizable to other, emergent tick-borne pathogens. Motivated by hypotheses regarding landscape context and vertebrate hosts, we asked how landscape context, namely habitat amount, quality, and fragmentation, is related to the distribution of Ehrlichia chaffeensis, a tick-borne pathogen of increasing concern, and the interannual occupancy dynamics of its vector, the lone star tick (Amblyomma americanum). We collected nymphal ticks from 130 plots in southeastern Virginia, U.S., for 5 years and tested for E. chaffeensis via targeted PCR. We derived metrics of landscape context from Landsat data and related these to pathogen prevalence and tick turnover using hierarchical Bayesian models. Landscape context was associated with both pathogen prevalence and tick turnover. Pathogen prevalence was negatively associated with total forest landcover, coniferous forest landcover, and forest edge density. Tick turnover was positively associated with coniferous landcover and with an interaction between total forest landcover and edge. This interaction was such that turnover was predicted to be lowest in small contiguous forests, and highest in small fragmented forests. Landscape context affects E. chaffeensis prevalence and occupancy dynamics of its tick host, though these processes appear decoupled. We hypothesize that pathogen prevalence may be more driven by reservoir host movement and social behavior and tick dynamics are more driven by host population density.

First report of Candidatus Rickettsia mendelii in Ixodes brunneus from the United States

Candidatus Rickettsia mendelii is a novel rickettsial species recently identified in Ixodes ricinus. In this study, Ixodes brunneus collected from wild birds (n = 77 ticks) or vegetation (n = 4 ticks) in southeastern Virginia were surveyed for rickettsial agents. Candidatus Rickettsia mendelii was confirmed in I. brunneus through sequencing of the gltA and 16S rRNA genes. This is the first report of this rickettsial species in Ixodes ticks in North America.

Greenhouse gas flux from stormwater ponds in southeastern Virginia (USA)

Abstract Stormwater ponds are ubiquitous features of developed landscapes of the eastern United States. Their design specifically controls the pace of water runoff from impervious cover of surrounding watersheds. Ponds accumulate organic matter that typically decomposes anaerobically in bottom sediments, and thus may be significant sources of greenhouse gases to the atmosphere (e.g., carbon dioxide (CO2), methane (CH4)). We sampled fifteen stormwater retention ponds in southeastern Virginia (USA) during summer 2018 to determine the diffusive emission of greenhouse gases with respect to measured environmental parameters. The equivalent CO2 (CO2e) flux from ponds ranged from 8.3 to 80 mmol m-2 h-1, with CH4 contributing 94%, CO2 6% and nitrous oxide less than 1% of the CO2e flux, on average. From linear mixed-effects modelling, diffusive flux of CO2 was inversely associated with pH. Maximum depth best explained diffusive flux of CH4, with surface area of secondary importance, i.e. CH4 flux was higher in smaller and more shallow ponds. With 300 stormwater ponds in the county where we conducted this study, we estimate that, during a 100-day warm season, these ponds emit 2.3 × 109 ± 1.5 × 109 SD g C as CO2e. As small, human-constructed ponds are becoming common features of urbanizing landscapes globally, results from this study suggest that, collectively, small ponds can contribute substantially to climate forcing. Better pond designs that reduce sediment methanogenesis, however, can mitigate the hypothesized potential disservice of GHG emissions from unvegetated stormwater retention ponds.

Observations on the terrestrial leech Haemopis septagon Sawyer amp; Shelley, 1976 (Annelida: Hirudinea) from the Outer Banks, North Carolina, USA,with a revision of the species.

The terrestrial leech Haemopis septagon Sawyer Shelley, 1976, is indigenous to the Great Dismal Swamp and environs of northeastern North Carolina and southeastern Virginia, USA. Ever since its discovery in 1895 at Lake Drummond in the Dismal Swamp, this elusive species has been recognized as taxonomically aberrant. For example, it is the only jawed leech in the United States with seven annuli between gonopores, and the only one with sixteen complete (5-annulate) segments, both highly conserved characters in the Hirudinidae. The discovery of two populations of H. septagon in the Albemarle Peninsula in the Outer Banks region of North Carolina afforded an opportunity to investigate the taxonomy and biology of this inadequately characterized species. Its description in this study is the first comprehensive account of the external and internal anatomy of this species since its incomplete original description in 1976. This study is also an opportunity to correct errors in the incomplete original description, and to elucidate morphological and developmental variability of taxonomic significance. Evidence is presented for the first time of a possible aquatic or semi-aquatic form of H. septagon. These Albemarle individuals were compared to the holotype from Durham County, NC, specimens from southeastern Virginia and a terrestrial leech recently reported from southern New Jersey. All of these fall within the variability demonstrated in this study for the Albemarle populations, and are accordingly recognized as the same species, H. septagon. Consequentially, Haemopis ottorum Wirchansky Shain, 2010, is recognized as a junior synonym of Haemopis septagon.