Purpose: The objective this study was to evaluate nitrogen fixation effectiveness, survival and compatibility of the isolates of on two different soils that came from Dabat and Shentia sites at Gondar, Ethiopia. The author recommends that the isolates test their nitrogen fixing potential under field conditions in slightly acidic and neutral soil before being used as commercial biofertilzer inoculants. The present study provides potential nitrogen fixer input for agricultural research centers. Methodology: Soil physicochemical analysis and most probable number were done according to their standard procedure. Three top strains were selected as inoculants for faba beans grown on the slightly acidic Shentia soil and the slightly neutral Dabat soil with their control. The symbiotic effectiveness of the strains was evaluated based on plant agronomy and total nitrogen of the plant. The results of the strains analyzed by SPSS version 26. Findings: The highest rhizobium population size was 5.8 x102 cells g-1 soil, while the lowest was 1.7 x102 cell g-1 soil observed at the Dabat and Shentia sites, respectively. There is limited nitrogen and phosphorus content other were enough. It was significant difference inter-strain difference in the all agronomic parameter of all treatments in soil compared to each based on agronomic parameter, but across soils there was no significant different except shoot fresh weight. The result of these studies showed that two Rhizobium leguminosarum bv.viciae isolates and other combinations of them had considerable effect on agronomic properties. Unique Contribution to Theory, Practice and Policy: The application of biological nitrogen fixation through Rhizobium inoculums is promoted as a solution to the problem of poor soil fertility in areas where legumes are cultivated. In most of the developing countries, including Ethiopia, biological nitrogen fixation technology has not fully flourished, so this study was initiated to evaluate fixation potential of isolates.
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