Soil Carbon Dioxide Research Articles

Mechanical termination of a perennial grain crop minimally impacts soil structure, carbon and carbon dioxide emissions

AbstractIntroductionMechanical termination of crops can negatively affect soil biological, chemical, and structural characteristics. Perennial crops do not require annual termination and can improve these same soil characteristics, which has catalysed interest in the development of new perennial crops. Advanced lines of the perennial grass intermediate wheatgrass (Thinopyrum intermedium [Host] Barkworth and Dewey; IWG) have been bred for increased seed size and marketed as Kernza® perennial grain, but little is known about how this new crop can be terminated for subsequent annual crop production in rotations that enhance agricultural productivity and environmental sustainability.Materials and MethodsFive methods of terminating IWG were tested in Minnesota, USA. Treatments included mechanical tillage using a chisel plow (CHI), undercutter (UND), and disc (DSC), along with no‐till treatments of glyphosate (GLY) and a repeated‐mowing control (CTRL). Treatment effects on IWG mortality, soil carbon dioxide (CO2) emissions, bulk density, aggregate stability, soil carbon stocks and soybean yield were measured.ResultsDaily CO2 fluxes differed by treatment (p < 0.05) on only one of 19 sample dates, and cumulative soil CO2 emissions over the course of the growing season did not differ across treatments. Bulk density decreased relative to baseline in all treatments except CTRL. Aggregate stability remained unchanged in all treatments except CTRL, which increased from the baseline. Soil carbon stocks did not change in any treatment. Soybean yield was highest in GLY but was not significantly different from CHI or UND.ConclusionsSoil structure, soil carbon stocks and soil CO2 emissions were unaffected by tillage and no‐till IWG termination treatments. However, tillage followed by preplanting harrowing proved ineffective at terminating IWG and required subsequent summer herbicide applications. Therefore, additional tillage events may be required to fully terminate IWG when herbicide use is prohibited.

The global‐scale impacts of metallic nanoparticles on soil carbon dioxide emissions

AbstractTerrestrial ecosystems are increasingly being exposed to metallic nanoparticles (MNPs). However, the global‐scale impact of MNPs on soil carbon dioxide (CO2) emission remains unknown, limiting our understanding of the role of MNPs in global carbon cycle. We compiled a dataset comprising 1764 pairs of experimental observations to investigate the effects of MNPs exposure on soil respiration parameters, including respiration rates, related enzyme activities, and microbial variables. We found that MNPs could stimulate or suppress soil basal respiration rates across global environments, depending largely on the type of MNPs. We further showed that, although the inhibition of MNPs on most enzyme activities peaked in short‐term exposure (≤28 days), prolonged exposure to MNPs still inhibited soil organic carbon degradation. Finally, we determined that environmental conditions (e.g., soil pH and presence/absence of plants) were also important regulators of the influence of MNPs on soil respiration parameters. Our findings underline that the effects of MNPs on soil CO2 emission depended on MNPs type, exposure design, and environmental factors, which is critical for predicting the role of MNPs in influencing the global carbon cycle and climate change.

Release of Carbon Dioxide and Water-Soluble Carbon Amended with Rice and Wheat Residues in an Inceptisols of Varanasi

ABSTRACT Crop residues are the main organic source of nutrients in agricultural soils. The active pool of soil organic carbon plays a dominant role in crop production and soil health. Rice and wheat residues were used in 90 days incubation study under controlled conditions (25°C temperature and maximum water-holding capacity) to estimate the release of carbon dioxide and water-soluble carbon in an Indo-Gangetic alluvial soil (Inceptisols) of Varanasi. The experiment was laid out in a factorial completely randomized design with three replications. The rice and wheat residues showed a wide variation in the content of their total carbon (TC), total nitrogen (TN), total phosphorus (TP) and total sulfur (TS). Out of the two crop residues added to the soil, rice residue had higher carbon dioxide-carbon (CO2-C) release than that of the wheat residue over control. Results revealed that conjunctive application of rice residue along with urea (20 kg N ha−1) and Trichoderma viride (10 kg ha−1) had resulted in a higher content of CO2-C (7.44 mg per 100 g per day) at 90 days of the incubation period. The amount of CO2 (mg) released, increases with a combination of crop residues with urea (20 kg N ha−1) and Trichoderma viride (10 kg ha−1) up to 90 days over control. The highest release of CO2 (204.70 mg) was recorded with rice residue treated with urea (20 kg N ha−1) and Trichoderma viride (10 kg ha−1). The study also revealed that the release of water-soluble carbon decreases with an increase in the incubation period irrespective of treatment. At 90 days of the incubation period, the control without any crop residue addition has the highest water-soluble carbon content (459.75 µg/g). The present study indicates that the incorporation of rice and wheat residues along with urea and supplemental decomposers (Trichoderma viride) has the potential to release adequately active organic carbon pool parameters such as CO2 and water-soluble carbon in an Inceptisols of Varanasi region of India.

Long term response and adaptation of farmland water, carbon and nitrogen balances to climate change in arid to semi-arid regions

Climate change poses a challenge for resource utilization and environmental pollution issues caused by agricultural production, especially in arid to semi-arid regions. Farmland water, carbon and nitrogen balances are closely related to these resource and environmental issues. Thus, the Agro-Hydrological & chemical and Crop systems simulator was used to assess the response of water, carbon and nitrogen balances to climate change in a spring wheat farmland of arid to semi-arid Northwest China and to propose adaptation strategies. Five Global Climate Models from the Coupled Model Intercomparison Project 6 and two Shared Socioeconomic Pathways (SSP1–2.6 and SSP5–8.5) were used to establish scenarios with the Agro-Hydrological & chemical and Crop systems simulator to simulate farmland water, carbon and nitrogen balances for the 2025–2100 period. Various irrigation amounts and nitrogen fertilization rates were tested as compensation strategies. Results indicated that climate change could negatively affect farmland water, carbon and nitrogen balances, especially under the SSP5–8.5 scenario. Precipitation showed an increasing trend, thus percolation increased and soil water consumption decreased from 2025 to 2100. However, for the carbon budget, although the soil carbon dioxide emissions tend to decrease, the net primary production was also significantly reduced, which resulted in declining the net ecosystem carbon budget under future climatic conditions. In addition, higher temperature and increased precipitation enhanced soil inorganic nitrogen leaching and nitrous oxide emissions but reduced ammonia volatilization from 2025 to 2100. Overall, the soil total nitrogen loss was increased over time, whereas crop nitrogen uptake was significantly reduced. In relation to the SSP1–2.6 scenario, the SSP5–8.5 scenario accelerated the increase rates of soil water percolation and total nitrogen loss over time, as well as the decrease rates of crop nitrogen uptake and net primary production over time. The negative effects caused by climate change can be mitigated by reducing irrigation and increasing nitrogen fertilization. For the SSP1–2.6 scenario, 30% irrigation reduction and 30% nitrogen fertilization increase can effectively decrease soil water percolation and the related nitrogen losses while crop nitrogen uptake, net primary production and net ecosystem carbon budget increase in relation to the current management (irrigation = 240 mm and nitrogen fertilization = 200 kg ha–1). For SSP5–8.5 the strategy with 45% irrigation reduction and 45% nitrogen fertilization increase can also decrease nitrogen losses and increase crop nitrogen uptake, net primary production and net ecosystem carbon budget.

Post-fire soil carbon emission rates along boreal forest fire chronosequences in northwest Canada show significantly higher emission potentials from permafrost soils compared to non-permafrost soils

Boreal forests are one of the most important biomes storing carbon (C). Wildfires burn yearly on average more than 1% of the boreal forest, and it is expected that the fire return intervals will shorten due to climate change. Fire is one of the most influential factors affecting soil organic matter quantity and quality, soil C pools, and presumably also the time C resides in the soil (soil C turnover time in years). We compared the potential effects of forest fire through post-fire succession on soil carbon dioxide (CO2) emission rates and soil C turnover time in two fire chronosequences, one with underlying permafrost soil and the other without permafrost. We found that fire had a significant effect on potential soil C turnover times, but surprisingly there was no significant difference in soil C turnover times between the permafrost and non-permafrost areas, although the soil CO2 emissions rates in permafrost areas are approximately three times higher compared to non-permafrost areas. In recently burned areas the potential soil C turnover times were two times longer compared to control areas located in forests burned more than 100 years ago. The longest potential soil C turnover times were recorded in mineral soil layers (30 cm) of permafrost soils, and the shortest potential soil C turnover times were recorded in humus layers of non-permafrost areas.

Spatially-temporal distribution of moisture content and dynamics of greenhouse gas emissions from upper soil horizons in floodplain fallow lands of Bashmakovsky district of Penza oblast

There are more than 1’300 thousand hectares of fallow lands potentially suitable for agricultural producers in the Penza region of Russia, which is 31% of the region total area. More than 300000 hectares of land have been abandoned for more than 20 years, and territory already became forest. Additionally soils under young forests – actively sequester carbon dioxide in soil. How much it makes sense to raise fallow land in terms of climate change and which areas produce the most carbon dioxide from the soil is a research question of great interest. In addition to vegetation, factors such as soil moisture and temperature influence the carbon dioxide emission from soil. As a result, the greenhouse gas fluxes monitoring, we can conclude that forest areas and natural ecosystems mostly deposit carbon dioxide, as the amount of available carbon increases due to a greater increase in biomass. Areas that are used in agriculture produce more nitrous oxide and methane, but less carbon dioxide, which is associated with the specifics of fertilization.

Carbon Sequestration and Climate Change

The long-term storage of carbon dioxide in soils, vegetation, oceans, and geological formations, with the objective of reducing or postponing global warming, is known as carbon sequestration. It reduces the amount of greenhouse gas emitted by various human activities. Scientists estimate that there is a direct correlation between rising global temperatures and atmospheric carbon dioxide levels, with the atmosphere containing 30% more carbon today than it did 150 years ago. One recommended approach to reducing atmospheric carbon dioxide is to increase carbon storage globally through a variety of means. To slow the net rate of increase in atmospheric CO2, carbon sequestration involves storing CO2 in long-lived global pools found in forests, oceans, biomass, and geological strata. This process is crucial for the maintenance of the global carbon cycle. To combat climate change, the global community must lower carbon emissions and increase net carbon sequestration. These methods can reduce the risks associated with climate change, but it is important to weigh the advantages and disadvantages of each suggested carbon sequestration strategy. The present study attempts to discuss various methods of carbon sequestration to reduce global warming.

Effect of combined application of inorganic nitrogen and phosphorus to an organic-matter poor soil on soil organic matter cycling.

Sequestering carbon dioxide (CO2) in agricultural soils promises climate change mitigation as well as sustainable ecosystem services. In order to stabilize crop residues as soil carbon (C), addition of mineral nutrients in excess to crop needs is suggested as an inevitable practice. However, the effect of two macronutrients i.e., nitrogen (N) & phosphorus (P), on C cycling has been found contradictory. Mineral N usually decreases whereas mineral P increases the soil organic C (SOC) mineralization and microbial biomass. How the addition of these macronutrients in inorganic form to an organic-matter poor soil affect C cycling remains to be investigated. To reconcile this contradiction, we tested the effect of mineral N (120 kg N ha-1) and/or P (60 kg N ha-1) in presence or absence of maize litter (1 g C kg-1 soil) on C cycling in an organic-matter poor soil (0.87% SOC) in a laboratory incubation. Soil respiration was measured periodically during the incubation whereas various soil variables were measured at the end of the incubation. Contrary to literature, P addition stimulated soil C mineralization very briefly at start of incubation period and released similar total cumulative CO2-C as in control soil. We attributed this to low organic C content of the soil as P addition could desorb very low amounts of labile C for microbial use. Adding N with litter built up the largest microbial biomass (144% higher) without inducing any further increase in CO2-C release compared to litter only addition. However, adding P with litter did not induce any increase in microbial biomass. Co-application of inorganic N and P significantly increased C mineralization in presence (19% with respect to only litter amended) as well as absence (41% with respect to control soil) of litter. Overall, our study indicates that the combined application of inorganic N and P stabilizes added organic matter while depletes the already unamended soil.

Effects of microplastics on soil C and N cycling with or without interactions with soil amendments or soil fauna

AbstractMicroplastics (MPs) enter the global soil ecosystem in significant quantities, and in agricultural lands, interact with soil amendments (e.g, fertilisers, pesticides, biochar), pollutants (e.g., heavy metals and acid rain) and soil fauna (e.g., earthworms and microbial biomass) which impacts carbon (C) and nitrogen (N) cycling in soil in ways that are largely unexplored. Here, we analysed the difference in the responses of soil C and N contents, greenhouse gas emissions and soil enzyme activities in experiments where MPs alone or MPs in combination with other soil amendments or soil fauna had been explored, by conducting a global meta‐analysis of 2543 observational data extracted from 84 published studies. The results show that MPs alone are associated with significantly increased soil C storage and altered soil N pool composition, increased soil carbon dioxide (CO2) and nitrous oxide (N2O) emissions, reduced soil ammonia (NH3) emissions, increased activity of one of the five major enzymes related to C cycle, and increased activity of three of the four major enzymes related to N cycling in soil. Experiments that explored the combined effects of MPs combined with fertilisers on soil C and N contents and enzyme activities indicated greater effects than MPs alone. However, the effects of MPs combined with soil fauna, heavy metals, acid rain, glyphosate, and carbon nanotubes on soil C and N cycling differed from findings of studies that considered the effects of MPs alone. The results of this meta‐analysis provide a theoretical basis for further study of the effects of MPs on C and N cycling in fertilized agricultural soil, compound‐contaminated soil and acidified soil. We also show that, despite the global importance of MPs in soil, the number of studies of their effects on soil C and N cycling is relatively few, and further research on the mechanisms of interactions of MPs with multiple soil amendments in soil systems is urgently needed.

Carbon dioxide emission estimation in different zones of the south of Western Siberia

The research was carried out in key areas of fallow land in three natural agricultural zones of the south of Western Siberia in the Omsk Oblast: steppe (Cherlaksky district, v. Preobrazhenka), forest-steppe (Kormilovsky district, v. Sosnovka), subtaiga (Tarsky district, Tara); in each zone there are three sites with fallow land of different ages: 5-10, 10-20 and 20-30 years. When comparing the intensity of carbon dioxide emission from fallow lands depending on the conditions of natural and climatic zones, it can be noted that its highest value was in the subtaiga zone (average for April-September 39.4 CO2/m2 per day), which is higher than the emission in the forest-steppe zone (14.7) and steppe zone (16.6). The maximum intensity of fluxes was observed in the subtaiga zone in August, and in the forest-steppe and steppe zones in July. One of the main reasons for this pattern was the air and soil temperature: the higher it is, the more intensive is the release of carbon dioxide. The influence of the age of fallow land on the level of soil carbon dioxide emission is observed. Younger fallows are characterized by a higher value of CO2 emission compared to older ones, so in the subtaiga zone the average seasonal emission of fallows of 5-10 years is 1.51 times higher than fallows of 20-30 years, in the forest-steppe zone - 1.46 times, steppe zone - 1.48 times.

ПИЛОТНЫЙ КАРБОНОВЫЙ ПОЛИГОН: АНАЛИЗ ЗАПАСОВ УГЛЕРОДА В ПОЧВАХ СЕЛЬХОЗУГОДИЙ

The carbon cycle in agroecosystems is determined by the balance between the absorption of carbon dioxide by terrestrial vegetation of agricultural crops to create organic matter and its release during soil respiration. The soil cover is a powerful source of carbon dioxide and serves as a reservoir accumulating soil organic carbon. Organic carbon accumulating in the humus of soils serves as a drain of carbon dioxide, methane into the atmosphere for hundreds of years. The research was carried out in order to analyze the reserves of organic carbon in the soils of agroecosystems and assess the emission of CO2 and CH4 gases. The work was carried out in 2022-2023 at the site of a pilot carbon landfill (arable land) in the Republic of Bashkortostan. In field and laboratory experiments, technologies for controlling carbon dioxide emissions on agricultural lands were developed and tested. In the field, full-profile soil sections and digs were laid in accordance with GOST R58595-2019 with the determination of morphological properties and the selection of soil samples for laboratory analysis. Agrochemical analysis of the soil for the content of organic matter, the density of addition, the content of mobile phosphorus and potassium, the pH value allowed us to estimate the carbon stock of the soil of the carbon landfill according to the methodology of the Ministry of Natural Resources of the Russian Federation. The total reserves of organic carbon of the arable horizon at the carbon-new landfill amount to 503.8 t/ha, which vary and depend on the employment of the field and the characteristics of the agricultural crop. Measurement of the destructive part of the carbon cycle using a portable LI-COR 7810 camera showed that the fields occupied by crops differ in the intensity of the release of soil carbon dioxide ‒ in the field under pure steam, CO2 emissions were 2.18 times lower than under perennial grasses. The availability of data on the spatial distribution of organic soil carbon makes it possible to introduce carbon-dependent crop rotations with a set of crops that contribute to the maximum absorption of atmospheric CO2 and other greenhouse gases into the practice of agriculture.

Carbon Mineralization Kinetics and Soil Fertility Induced by Organic and Bio-Fertilization Through Different Incubation Periods

ABSTRACT Agricultural practices such as fertilization considerably influence soil carbon dioxide (CO2) fluxes that cause climate changes. The study aims to test the influence of various organic (vermicompost, VC, and rabbit manure, RM) and bio (Azotobacter and Azospirillum) fertilizers in addition to incubation times on CO2flux, carbon (C) mineralization kinetic, and some soil chemical properties. The dual exponential equation was the best one to describe carbon mineralization kinetics compared to the 1st and 2nd order equations. Carbon half-lives were resided by 18.98, 1.97, 2.00, 1.89, and 2.53 years for control, VC without and with bio, RM without and with bio treatments, respectively. The results exposed that applying VC and RM without or with bio-fertilizer reduced soil reaction (pH) that differ than control treatment after 2 months incubation. Applying VC and RM caused a significant increase in soil salinity (EC) compared to the control. Available nitrogen (N), phosphorus (P) and potassium (K) were significantly enhanced with adding different fertilizers compared to pre-treated soil. It is found that adding organic and bio-fertilizers improved fertility and soil quality.

Environmental controls of winter soil carbon dioxide fluxes in boreal and tundra environments

Abstract. The carbon cycle in Arctic–boreal regions (ABRs) is an important component of the planetary carbon balance, with growing concerns about the consequences of ABR warming for the global climate system. The greatest uncertainty in annual carbon dioxide (CO2) budgets exists during winter, primarily due to challenges with data availability and limited spatial coverage in measurements. The goal of this study was to determine the main environmental controls of winter CO2 fluxes in ABRs over a latitudinal gradient (45∘ to 69∘ N) featuring four different ecosystem types: closed-crown coniferous boreal forest, open-crown coniferous boreal forest, erect-shrub tundra, and prostrate-shrub tundra. CO2 fluxes calculated using a snowpack diffusion gradient method (n=560) ranged from 0 to 1.05 g C m2 d−1. To assess the dominant environmental controls governing CO2 fluxes, a random forest machine learning approach was used. We identified soil temperature as the main control of winter CO2 fluxes with 68 % of relative model importance, except when soil liquid water occurred during 0 ∘C curtain conditions (i.e., Tsoil≈0 ∘C and liquid water coexist with ice in soil pores). Under zero-curtain conditions, liquid water content became the main control of CO2 fluxes with 87 % of relative model importance. We observed exponential regressions between CO2 fluxes and soil temperature in fully frozen soils (RMSE=0.024 gCm-2d-1; 70.3 % of mean FCO2) and soils around the freezing point (RMSE=0.286 gCm-2d-1; 112.4 % of mean FCO2). FCO2 increases more rapidly with Tsoil around the freezing point than at Tsoil<5 ∘C. In zero-curtain conditions, the strongest regression was found with soil liquid water content (RMSE=0.137 gCm-2d-1; 49.1 % of mean FCO2). This study shows the role of several variables in the spatio-temporal variability in CO2 fluxes in ABRs during winter and highlights that the complex vegetation–snow–soil interactions in northern environments must be considered when studying what drives the spatial variability in soil carbon emissions during winter.

Biyokömür Karıştırma Zonu Toprak Karbondioksit Emisyonu Üzerinde Etkilidir

Several factors are effective on soil carbon dioxide emissions caused by agricultural practices; soil organic matter contents, soil moisture and temperature, climatic changes, and tillage techniques are predominant. In recent years, as a CO2 sequestration agent, biochar incorporation becomes a promising approach. Many studies show that biochar reduces soil CO2 emissions; however, incorporation depth is not widely studied. A pot experiment was carried out to determine the effects of the incorporation zone of rose pulp biochar produced at 400 C on carbon dioxide emission. Treatments were Z as without biochar incorporation (control), A, B, and C are the incorporation zone of 0-7, 0-14, and 0-21 cm soil layer. The measurements in the experiment last for about 2 months. Results revealed that mean CO2 emissions for Z, A, B, and C treatments were 0.048, 0.052, 0.064, and 0.076 g m-2h-1, respectively. According to these results, it was determined that the biochar admixed in the C layer caused more soil CO2 emissions, and there was no significant difference between the other treatments (p>0.05). The highest plant biomass development was obtained in the B treatment (p<0.05).

Acidified manure and nitrogen-enriched biochar showed short-term agronomic benefits on cotton–wheat cropping systems under alkaline arid field conditions

Application of organic residues such as farm manure and biochar in various agricultural environments have shown positive effects on soil carbon sequestration. However, there is a lack of consensus regarding the agronomical benefits of a single and small dose of biochar and farm manure in arid alkaline soils. Therefore, a field experiment with the given treatments (1) control (no amendment), (2) acidified manure (AM) at 300 kg ha−1, (3) nitrogen (N) enriched biochar (NeB) at 3 Mg ha−1, and (4) an equal combination of AM + NeB (150 kg ha−1 AM + 1.5 Mg ha−1 NeB)) was conducted in a typical cotton–wheat cropping system. A parallel laboratory incubation study with the same amendments was carried out to account for soil carbon dioxide emission (CO2). The N enrichment of biochar and its co-application with acidified manure increased soil mineral N (NO3− and NH4+) in the topsoil (0–15 cm), and increased total N uptake (25.92% to 69.91%) in cotton over control, thus reducing N losses and increased uptake over control. Compared to the control, co-application of AM + NeB significantly improved soil N and P bioavailability, leading to increased plant biomass N, P, and K (32%, 40%, 6%, respectively) uptake over control. The plant's physiological and growth improvements [chlorophyll (+ 28.2%), height (+ 47%), leaf area (+ 17%), number of bolls (+ 7%), and average boll weight (+ 8%)] increased the agronomic yield in the first-season crop cotton by 25%. However, no positive response was observed in the second season wheat crop. This field study improved our understanding that co-application of acidified manure and N-enriched biochar in small dose can be a strategy to achieve short-term agronomic benefits and carbon sequestration in the long run.

Reagentless reduction of hydrogen carbonate-calcium hardness of water: equilibrium states and kinetics

The equilibrium states and kinetics of changes in the concentrations of carbonate system components in water with different calcium hardness are analyzed and the possibility of reducing this indicator by an environmentally friendly method, aeration, is evaluated. Changes in the concentrations of dissolved carbon dioxide, calcium ions, hydrogen ions (pH), bicarbonate and carbonate ions in water depending on the partial pressure of carbon dioxide in soil and atmospheric air and the kinetics of reducing the bicarbonate-calcium hardness of water during its natural and forced aeration were traced. It is shown that water purification by a stream of atmospheric air microbubbles with an average radius of 50 m and a flow rate of 10 l/min can reduce the hydrogen carbonate-calcium hardness from 8 mg-eq/l to 1 mg-eq/l in 100 liters of water in 20 minutes. In the paradigm of "green chemistry", this method is more rational, environmentally friendly, and economical than ion exchange or reverse osmosis, which are currently practiced to reduce hardness in decentralized water supply systems.

Respiration and carbon use efficiency characteristics of soluble protein-derived carbon by soil microorganisms: A case study at afforested sites

Soluble protein plays a significant role in the release of carbon dioxide (CO2) in soils. However, our understanding of the respiration and C use efficiency (CUE) characteristics of soluble protein-derived C by soil microorganisms is limited. To address this issue, we sampled surface soils (0−10 cm) from seven tree monocultures and examined the temporal dynamics of turnover of 14C-labelled soluble protein-derived C by soil microorganisms. Two double first-order exponential kinetic decay models were applied to analyze the mineralization data (i.e., with and without abiotic protein-surface interactions). The model incorporating the immobilization of protein on the non-living solid phase exhibited the best fit to the experimental data (R2 > 99.6%). Our results suggest that 66.1−73.9% of the soluble protein-derived C was immobilized by the non-living solid phase in soils. After uptake by the soil microbial community, 8.0−13.8% of the C was rapidly respired as CO2, while 15.0−20.8% was used in anabolic processes, resulting in a CUE of 55.1–70.2%. However, there was little effect of forest type on protein turnover rate in the soil. The C:N ratio of soil microbial biomass (C/Nmic) was positively related to the CUE of protein and exhibited less variation within a forest type. Compared with soil microbial biomass C and N, C/Nmic could serve as a better indicator of the CUE of protein by soil microorganisms. This study sheds light on the respiration and CUE characteristics of soluble protein-derived C by soil microorganisms at afforested sites and enhances our understanding of the trade-off between the metabolism of protein-derived C by soil microorganisms and its immobilization by the non-living solid phase in soils.

Divergent effects of biochar amendment and replacing mineral fertilizer with manure on soil respiration in a subtropical tea plantation

Applying biochar amendment and manure in tea plantation ecosystems can diminish soil acidification and degradation. However, the impact of these practices on soil respiration and associated mechanisms remains unclear. In this study, we combined a two-year field experiment and laboratory analyses based on soil properties, functional genes, and microbial co-occurrence networks to explore the determinants of soil respiration intensity in a subtropical tea plantation with biochar amendment and manure application. The results showed that the effect of biochar amendment on soil respiration was unconspicuous. Although biochar amendment increased bacterial richness and Shannon index, biochar amendment did not alter the abundance of species associated with C-cycling functional genes. Besides directly adding recalcitrant C to the soil, biochar also indirectly enhanced C sequestration by weakly increasing soil carbon dioxide (CO2) emissions. However, replacing mineral fertilizer with manure significantly stimulated soil respiration in the tea plantation, resulting in a 36% increase in CO2 emissions over two years. The increase in CO2 emissions under the manure treatment was mainly attributed to the increased soil labile C pool, the activity of hydrolytic enzymes (e.g., cellobiohydrolase and acetylglucosaminidase), and the relative abundance of functional genes associated with the C-cycle. This may also be related to the application of manure that increased the abundance of Gemmatimonadetes and altered ecological clusters in bacterial co-occurrence networks. Our correlation network analysis suggested that Gemmatimonadetes might be the potential hosts for C-cycling genes due to their strong positive correlation with the abundance of C-cycling genes. Overall, these findings provide new insights into soil respiration under biochar amendment and manure application in tea plantations and broaden the options for carbon sequestration in soils.Graphical

Soil carbon mineralization, enzyme activities, and crop residue decomposition under varying soil moisture regimes

AbstractIn the Lower Mississippi River Basin, farmlands are flooded in late fall and winter by impeding drainage to provide a habitat for migratory birds stopover. A laboratory incubation study was conducted to study the influence of temporary wetting of these soils on carbon (C) and nitrogen (N) mineralization from residue decomposition. Surface soils (0–15 cm) were collected from fields with clay and silty clay loam soils. Both soils were incubated with five crop residues—corn (Zea mays), soybean (Glycine max), cotton (Gossypium hirsutum), sorghum (Sorghum bicolor), and sweet potato (Ipomea batatus)—at 50% and 100% water holding capacity (WHC). Soil carbon dioxide (CO2) efflux was measured during 74 days of incubation. Soil moisture had the largest effect on soil CO2 efflux and most of the enzyme activities followed by residue and soil type. Cumulative soil CO2 efflux was reduced by 46% at 100% WHC compared to 50% WHC. Clay soil had higher cumulative CO2 efflux, aryl sulfatase, aryl phosphatase, and β‐N‐acetyl glucose aminidase activities than silty clay loam soil. Residue type had the largest effect on post‐incubation soil inorganic‐N, remaining biomass, and residue C and residue N. Sweet potato residue had the lowest remaining biomass, significantly lower than cotton, corn, and sorghum, but statistically similar to soybean. After incubation, residue N concentration had a positive association with cumulative CO2 efflux and a negative association with remaining residue biomass percentage. Winter flooding might limit the loss of soil C as CO2 depending on the crops grown and soil type.

Double-cropping, tillage and nitrogen fertilization effects on soil CO2 and CH4 emissions

Double-cropping is increasingly embraced for its economic advantages in irrigated crops within the Ebro Valley region. However, the implications of this practice for soil carbon dioxide (CO2) and methane (CH4) emissions remain poorly understood. This study aimed to assess the impact of legume-maize double-cropping on soil CO2 and CH4 emissions and to identify optimal tillage systems and nitrogen fertilization rates for enhancing soil carbon budget. Conducted in Agramunt (NE Spain), the research transformed a long-term tillage and nitrogen fertilization field experiment initiated in 1996 under rainfed conditions. It transitioned into a maize monocrop system in 2015 and a diversification experiment in 2018. Maize monocrop was compared to a legume-maize double-cropping with two tillage systems (conventional tillage and no-tillage) and three mineral nitrogen fertilization rates (zero, medium and high). The legumes used were pea for grain (2019), vetch for green manure (2020) and vetch for forage (2021). The use of legumes in the double-cropping system had a significant impact on CO2 fluxes. CO2 emissions in the double-cropping ranged from 250 to 8070 mg CO2-C m−2 day−1, while in the monocropping, the fluxes were in the range of 150–5790 mg CO2-C m−2 day−1. Due to the higher biomass production and extended crop cycle in the double-cropping, along with increased residue decomposition due to the higher amount of residue provided, the cumulative CO2 emissions were higher in the double-cropping. Within the double-cropping, the response of cumulative soil CO2 emissions to tillage depended on the legume used and residue management. For legumes with a low nitrogen content, conventional tillage resulted in lower cumulative CO2 emissions, while for legumes with a high nitrogen content, the lowest emissions were observed in the no-tillage system. The increased incorporation of crop residues in the combined approach of double-cropping and no-tillage led to a greater net ecosystem carbon budget, reaching 1960 kg C ha−1. However, the use of high or medium nitrogen fertilization rates did not have a significant impact on CO2 emissions or the net ecosystem carbon budget. Cumulative soil CH4 emissions were only affected by soil tillage in 2019 and 2021, with greater net CH4 uptake under no-tillage compared with conventional tillage. This work underlines the importance of using legume-maize double-cropping together with no-tillage and N fertilization reduction to control the soil CO2 and CH4 emission yields, while maintaining crop yields under Mediterranean conditions.