Concentrations Of Sodium Lauryl Sulfate Research Articles

Impact of surfactant on the pore and particle sizes of copolymer (2‐acrylamido‐2‐methylpropane sulfonic acid/acrylamide) nanohydrogels for controlled release of 5‐fluorouracil

Nanohydrogels were prepared from copolymerization of acrylamido‐2‐methylpropane sulfonic acid (AMPS) and acrylamide (AAm) in presence of sodium lauryl sulfate (SLS). The direct ionizing radiation of γ‐rays was used to initiate the copolymerization reaction. The different concentrations of SLS, variable irradiation doses, a fixed total monomer concentration, and comonomer composition were used. The features of nanohydrogel were characterized by Fourier transform infrared, thermal gravimetric analysis, and BET surface area analysis of porosity. The results confirmed the structure formation of copoly(AMPS/AAm) nanohydrogels. The SLS concentration affects clearly the pore characteristics and nanoparticle sizes. The nanoparticle and pore size distributions referred to that the pores and nanoparticle formed in wide range of nanoscale. The surface area and pore volume depended basically on the SLS concentration. The gel fraction of nanohydrogels ranged from (98 ± 2.3) to (100 ± 1.5)%. The nanohydrogels were used in in vitro release of 5‐fluorouracil (5‐FU) at different simulated fluids. The release profile showed a prolonged release of 5‐FU at two different simulated fluids of pHs of 1.2 for simulated gastric fluid and 7.4 for simulated intestinal fluid. The study demonstrated that copoly(AMPS/AAm) nanohydrogel may serve as a promising material for the sustained release of 5‐FU in stomach and colon media. POLYM. ENG. SCI., 58:94–102, 2018. © 2017 Society of Plastics Engineers

Effects of different base agents on prediction of skin irritation by sodium lauryl sulfate using patch testing and repeated application test

Animal testing for cosmetics was banned in the European Union (EU) in 2013; therefore, human tests to predict and ensure skin safety such as the patch test or usage test are now in demand in Japan as well as in the EU. In order to investigate the effects of different bases on the findings of tests to predict skin irritation, we performed patch testing (PT) and the repeated application test (RAT) using sodium lauryl sulfate (SLS), a well-known irritant, dissolved in 6 different base agents to examine the effects of these bases on skin irritation by SLS. The bases for PT were distilled water, 50% ethanol, 100% ethanol, a gel containing 50% ethanol, white petrolatum, and hydrophilic cream. The concentrations of SLS were 0.2% and 0.5%. Twelve different base combinations were applied to the normal back skin of 19 individuals for 24h. RAT was performed with distilled water, 50% ethanol, 100% ethanol, a gel containing 50% ethanol, white petrolatum, and hydrophilic cream containing SLS at concentrations of 0.2%, 2%, and 5%, being applied to the arms of the same PT subjects. The test preparation of each base was applied at the same site, with 0.2% SLS being used in the first week, 2% SLS in the following week, and 5% SLS in the final week. The results of PT revealed that skin irritation scores varied when SLS at the same concentration was dissolved in a different base. The results of RAT showed that although skin irritation appeared with every base at a concentration of 5%, the positive rate was approximately the same. In conclusion, our results suggest that skin irritation elicited in PT depends on the base, while in RAT, it does not depend on the type of base employed.

Template-Assisted Electrodeposition of Fe-Ni-Co Nanowires: Effects of Electrolyte pH and Sodium Lauryl Sulfate

The influence of pH and sodium lauryl sulfate (SLS) electrolyte composition on the potentiostatic, template-assisted electrodeposition of Fe-Ni-Co alloy nanowires into alumina nanoporous membranes with a large accompanying hydrogen side reaction is presented. Solid nanowires and tubular, composite tips were observed and the average alloy composition was characterized. The growth of nanowires was insensitive to SLS at pH values of 2.0, but was a strong function of SLS at a low pH of 0.5. Anomalous codeposition was evident and the Fe-rich alloy nanowires were independent of SLS concentration at pH values above 0.5. The change in deposition rate and alloy composition was attributed to changes in adsorbed species: metal ion intermediates, hydrogen ion, hydrogen gas bubbles and SLS.

Chemical mechanical planarization of Al alloy in alkaline slurry at low down pressure

Effects of hydrogen peroxide and inhibitors (sodium benzoate, NaNO3, sodium lauryl sulfate) on material removal at low down pressure for chemical mechanical planarization of Al alloy were investigated in alkaline slurry by using atomic force microscope, electrochemical polarization tests, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. The material removal rate initially increases with concentration of H2O2 owing to the increase in corrosion potential and in thickness of oxidization layer, and then reaches a constant value. Additionally, surface scratch and orange peel disappear with H2O2. Sodium lauryl sulfate (SLS) reduces the surface roughness while maintaining a reasonable material removal rate in comparison with sodium benzoate and NaNO3 due to decrease in passive current density and increase in thickness of passive layer. A weak passive layer is generated on Al alloy surface by adhesive of SLS with H2O2, which is easily removed at low mechanical abrasion. The chemical composition of the film layer is dominated by the Al oxide rather than sulfate though the thickness of the passive layer is increased with SLS concentration.

Optimization of RGD-modified Nano-liposomes Encapsulating Eptifibatide

Eptifibatide (Integrilin) is an intravenous (IV) peptide drug that selectively inhibits ligand binding to the platelet GP IIb/IIIa receptor. It is an efficient peptide drug, however has a short half-life. Therefore, antithrombotic agents like eptifibatide are required to become improved with a protected and targeted delivery system such as using nano-liposomes to the site of thrombus. The goal in the present report was to optimize encapsulation efficiency of the eptifibatide into Arg-Gly-Asp (RGD)-modified nano-liposomes (RMNL). As well, it was intended to evaluate the effect of sodium lauryl sulfate (SLS) on drug release. The effect of five independent variables including number of freeze/thawing cycles, concentration of eptifibatide, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, and dipalmitoyl-GRGDSPA peptide on drug entrapment efficiency (DEE) was investigated using response surface methodology (RSM). The effect of different concentrations of SLS on encapsulation and drug release from RMNL was also investigated. The size and morphology of RMNL were characterized using transmission electron microscopy (TEM). The maximum DEE (38%) was obtained with 7 freeze/thawing cycles, 3.65 mmoL eptifibatide, 7 mM DSPC, 3 mM cholesterol, and 1 mM dipalmitoyl- GRGDSPA peptide. SLS has significantly increased the drug release from RMNL, although its effect on encapsulation efficiency was not significant. The optimization of the formulations for valuable and expensive peptide drugs is essential to have the maximum encapsulation efficiency and the minimum experiments.

Dispersive liquid–liquid microextraction based on solidification of floating organic drop for separation and preconcentration of malachite green before its determination by flow injection spectrophotometry

ABSTRACTA simple and fast dispersive liquid–liquid microextraction based on solidification of floating organic drop has been developed for the separation and preconcentration of malachite green in water samples prior to its determination by flow injection spectrophotometry. Sodium lauryl sulfate, an anionic surfactant, was used for the ion-pair formation with malachite green. The factors affecting the ion-pair formation and extraction were optimized. Under the optimized conditions (volume of 1-undecanol as the extraction solvent, 40 μL; the volume of ethanol as the disperser solvent, 100 μL; sodium lauryl sulfate concentration, 7.5 × 10−7 mol L−1, and the pH of the sample, ∼3.0), the calibration graph was linear over the range of 0.8–25 µg L−1 with the detection limit of 0.3 µg L−1 and the preconcentration factor of 750. The relative standard deviation at 7 µg L−1 (n = 6) was found to be 2.1%. The developed method was successfully applied to the determination of malachite green in river water and fish farming water samples.

Effect of solubility enhancers on the release of Carbamazepine from Hydrophilic Polymer based matrix tablet

In the present study, an attempt has been taken to evaluate the effect of sodium lauryl sulphate (SLS) and glyceryl mono stearate (GMS) as solubility enhancers on the release profile of a poorly soluble drug, carbamazepine. Matrix tablets of carbamazepine were prepared by wet granulation technique using hydrophilic polymers (10% of Methocel K15 MCR and 10% of Methocel K100LV CR) as release controlling agents. Varying amounts of SLS and GMS were used in six different formulations to observe the impact on the release rate and mechanism of drug release. The dissolution study of carbamazepine from these extended release matrix tablets was conducted for 24 hours using basket method in 900 ml distilled water as dissolution medium. The data obtained from the dissolution studies were explored and explained with the help of zero order, Higuchi, first order, Korsmeyer- Peppas and Hixson-Crowell equations. It was found that the dissolution rate of carbamazepine with the formulation containing SLS was higher than GMS containing formulation and increased concentration of SLS increasing the release rate. Where there was no SLS or smaller amount of SLS or GMS release rate was decreased. Formulation having equal ratio of SLS and GMS did not show the desire release profile. The drug release mechanism followed mainly super case II transport. These results clearly demonstrated that the dissolution rate, extent and mechanism of carbamazepine release could be changed by optimizing the amount of SLS and GMS in the tablet formulation. DOI: http://dx.doi.org/10.3329/dujps.v13i2.21894 Dhaka Univ. J. Pharm. Sci. 13(2): 167-173, 2014 (December)

Partitioning Behavior of Gemifloxacin in Anionic, Cationic and Nonanionic Surfactants. Calculation of Dermal Permeability Coefficient

Transdermal delivery acts as an alternative to oral delivery of drugs and possibly provids also an alternative to hypodermic injection. Transdermal delivery when compared to oral route has a variety of advantages namely: avoiding the degradation of drugs in the stomach environment, providing steady plasma levels, avoiding first-pass metabolism, increaseing patient compliance, easy to use, non-invasive and inexpensive, increasing the therapeutic index with a simultaneous decrease in drug side effects. Despite these advantages, one of the greatest challenges to transdermal delivery is that only a limited number of drugs are amenable to administration by this route. Gemifloxacin, a broad spectrum fourth generation quinolone antibacterial agent has pharmacokinetic characteristics (particularly its low maximum plasma concentration, obtained following repeat oral dose of 320 mg) that makes it a potential target for transdermal delivery. The objective of the study was to explore the possibility of surfactants (anionic, cationic and nonionic) acting as dermal enhancers of gemifloxacin assuming that the drug is to be formulated into topical or transdermal pharmaceutical dosage form. To accomplish the objective, gemifloxacin was partitioned between chloroform and surfactants containing varying concentrations of sodium lauryl sulfate, cetyltrimethylammonium bromide, polysorbate-20 and polysorbate-80. The data obtained were used to estimate the dermal permeability coefficient. The partitioning was carried out by shake flask method at room temperature. It was observed that all the surfactants decreased the partition behavior of gemifloxacin when compared to that of water alone. Sodium lauryl sulfate produced the most decreasing partition effect at the highest concentration studied (2% w/v). The permeability coefficient (Kp) was estimated from the partition coefficient data and the molecular weight of the drug. As permeability coefficient is an important descriptor for evaluating dermal absorption of drugs employed in clinical treatment of various dermal accessible ailments, the results of the study suggest that the investigated surfactants might not be potential transdermal enhancers of gemifloxacin.

In Vitro Liberation of Indomethacin from Chitosan Gels Containing Microemulsion in Different Dissolution Mediums

The objective of this research is to outline the liberation of indomethacin from different chitosan gels containing O/W microemulsion. The influence of surfactant, sodium lauryl sulfate, in two concentrations (0.5% and 0.75%, w/w) was determined in dissolution medium on the release of indomethacin, which was used as poor water-soluble model drug. Chitosan gels were prepared in four different concentrations of chitosan-1%, 1.5%, 2%, and 3% (w/w). Microemulsion enhanced the liberation of the indomethacin from chitosan gels into all dissolution mediums. Adding the surfactant into phosphate-buffered saline decreased the amount of liberated indomethacin from microemulsion, gel mixture, but increased the drug liberation from pure chitosan gels. It was detected that with the increased concentration of chitosan in the samples, the amount of indomethacin liberated (p < 0.05) also increased. A conclusion was drawn that the liberation of indomethacin from chitosan gels was influenced by increased pH of the samples. The high viscosity induced a higher release of indomethacin from 3% (w/w) chitosan hydrogel at pH 5.8 as compared with 3% (w/w) chitosan hydrogel at pH 3.8. The highest percentage of released indomethacin was determined when a mixture of microemulsion gel with higher chitosan content was used.

Synthesis of silver nanoparticles and their antibacterial activity

In study, spherical Silver nanoparticles (SNPs) were synthesized by chemical reduction method from a metal precursor silver nitrate in presence of an anionic surfactant and strong reducing agent. In this experimental work, SNPs are synthesized in presence of different concentration of stabilizing agent and effect of stabilizing agent on size distribution of SNPs have been observed. Further antibacterial activities of the SNPs prepared under different concentration of sodium lauryl sulphate (SDS) carried out on gram negative and gram positive bacteria showed that as concentration of sodium lauryl sulphate increases antibacterial activity decreases.

Formulation design and evaluation of Cefuroxime axetil 125 mg immediate release tablets using different concentration of sodium lauryl sulphate as solubility enhancer

Cefuroxime axetil immediate release tablets were formulated by direct compression method with different percentages of sodium lauryl sulphate (SLS) such as 0.5, 1.0, 1.5 and also without SLS. Resulting batches of tablets were evaluated by both pharmacopeial and non-pharmacopeial methods to ascertain the physico-mechanical properties. Dissolution test were carried out in different medium like 0.07 M HCl, distilled water, 0.1M HCl of pH 1.2 and phosphate buffers at pH 4.5 and 6.8 to observe the drug release against the respective concentration of SLS used. Later, test formulations were compared by f1(dissimilarity) and f2(similarity) factors using a reference brand of cefuroxime axetil. Significant differences (p&lt;0.05) in dissolution rate were recorded with the change in concentration of SLS in different media. Test formulation T3 containing 1% SLS was found to be best optimized formulation based on assay, disintegration, dissolution and similarity and dissimilarity factors.

Characterizing the immunological effects of oral healthcare ingredients using an in vitro reconstructed human epithelial model

Oral healthcare products are well tolerated and while adverse occurrences are rare there is still a need to explore the interaction between these products and the oral mucosa. This study assessed the effects of oral healthcare ingredients: sodium lauryl sulphate (SLS), a detergent; cinnamic aldehyde (CA), a flavouring agent; and cetylpyridinium chloride (CPC), an antiseptic, using a reconstructed human oral mucosal model (OMM). Differential release of inflammatory cytokines IL-1α, IL-8 and cytotoxicity was compared with other known irritants and sensitizers to identify a signature response profile that could be associated with oral mucosal irritation.Response profiles differed with irritants being more cytotoxic. CA and control sensitizers nickel sulphate (NiSO4) and 1-chloro-2,4-dinitrochlorobenzene (DNCB) released lower levels of IL-1α than CPC and control irritant benzalkonium chloride (BC), whereas the opposite was observed for IL-8. Significant levels of IL-8 and IL-1α were released with 5–15 mg/ml (0.5–1.5% w/v) SLS. Quantitative PCR indicated that cytokine release at lower SLS concentrations is not entirely due to cell necrosis but in part due to de novo synthesis. These findings suggest that the OMM can be used to predict oral irritation thus making it a potentially valuable model for screening new oral healthcare ingredients prior to clinical release.

A Comparative Study of Three Cytotoxicity Test Methods for Nanomaterials Using Sodium Lauryl Sulfate

The biocompatibility evaluation of nanomaterials is essential for their medical diagnostic and therapeutic usage, where a cytotoxicity test is the simplest form of biocompatibility evaluation. Three methods have been commonly used in previous studies for the cytotoxicity testing of nanomaterials: trypan blue exclusion, colorimetric assay using water soluble tetrazolium (WST), and imaging under a microscope following calcein AM/ethidium homodimer-1 staining. However, there has yet to be a study to compare each method. Therefore, in this study three methods were compared using the standard reference material of sodium lauryl sulfate (SLS). Each method of the cytotoxicity test was carried out using mouse fibroblasts of L-929 exposed to different concentrations of SLS. Compared to the gold standard trypan blue exclusion test, both colorimetric assay using water soluble tetrazolium (WST) and imaging under microscope with calcein AM/ethidium homodimer-1 staining showed results that were not statistically different. Also, each method exhibited various advantages and disadvantages, which included the need of equipment, time taken for the experiment, and provision of additional information such as cell morphology. Therefore, this study concludes that all three methods of cytotoxicity testing may be valid, though careful consideration will be needed when selecting tests with regard to time, finances, and the amount of information required by the researcher(s).

UTILITY OF HADKAR FACTOR IN THE DETERMINATION OF CRITICAL MICELLE CONCENTRATION OF SURFACTANTS

The drop weight method was used to determine the interfacial tension between organic liquid and solutions of different concentrations of the surfactant, sodium lauryl sulphate (SLS), using Hadkar factor. Two experiments were performed to determine the interfacial tension. In the first experiment the drop weight method was used to determine the interfacial tension between water or aqueous surfactant solutions and benzene (liquid lighter than water) using Hadkar factor H1. In the second experiment the drop weight method was used to determine the interfacial tension between water or surfactant solutions and carbon tetrachloride (liquid heavier than water) using Hadkar factor H2. The plot of interfacial tension between benzene or CCl4 and SLS solutions versus concentration of surfactant solution was used to determine the critical micelle concentration (CMC) of the surfactant. The results were in good agreement with the reported values of CMC for SLS showing the utility of Hadkar factor in the determination of CMC of surfactants.

S100B as a potential biomarker for the detection of cytotoxicity of melanocytes

Skin irritation is one of the most common adverse reactions in hydroquinone (HQ) and retinoic acid (RA). Although melanocytes have rarely been considered to be involved in skin irritation, RA and particularly HQ could induce melanocyte toxicity, resulting in depigmentation. We chose S100B as a candidate gene for melanocytotoxicity from a genome-wide transcriptional profiling analysis after applying irritant doses of HQ, RA and sodium lauryl sulphate (SLS) to cultures of keratinocytes and/or melanocytes. In this study, the role of S100B on melanocyte viability and cytotoxicity was examined. S100B was detected in melanocytes, but not in keratinocytes or fibroblasts. Melanocytes after treatment with increasing concentrations of HQ, RA, SLS and urushiol showed significant increases in intracellular and extracellular S100B expression with reduced viable cell number and increased release of lactate dehydrogenase. No RAGE expression and no significant function of CD166/ALCAM in melanocyte survival and cytotoxicity favoured the role of intracellular S100B in chemically irritated melanocytes. S100B knock-down increased apoptosis through inhibition of PI3K/AKT, NF-κB and ERK activation, suggesting the increased intracellular S100B expression by chemical irritation as a compensatory reaction to reduce cytotoxicity. The numerical decrease in S100B/c-kit-double-positive melanocytes was also examined in human skin epidermis irritated by HQ or RA with stronger staining intensities of S100B. Collectively, the decrease in viable cell number by reduced intracellular S100B levels in vitro and by chemical irritation in vivo suggests that S100B could be a potential biomarker for melanocytes cytotoxicity.

Pool boiling enhancement with environmentally friendly surfactant additives

Boiling heat transfer is used in variety of industrial processes and applications, such as refrigeration, vapor cycle power generation, heat exchangers, petroleum refining, and chemical manufacturing. Enhancements in boiling heat transfer processes are critical for making these applications more energy efficient. The aim of this paper is to demonstrate the water pool boiling phenomena under the influence of environmentally friendly surfactant additives. The test setup used in this study has multiple benefits. First, the test setup enhances teaching in variety of classes through in-class demonstrations and student experiments. An experiment is described to allow the students to visualize and qualify different modes of boiling heat transfer. In addition, the test setup provides a platform for research in boiling enhancement. Using surfactant additives in boiling causes increased number of nucleation sites and decreased level of wall superheat. For determining surfactant effects, different concentrations of sodium lauryl sulfate (SLS), ECOSURF™ EH-14, and ECOSURF™ SA-9 are added to pure water and enhancement through surfactants is quantified. When times until boiling (liquid temperature reaches the boiling point) are measured, 17, 10.3, and 19.6 % lower times found (for SLS, EH-14, and SA-9, respectively) compared to pure water. Wall temperature reduction is measured for 50 ppm SLS 9.48 %, for 300 ppm EH-14 11.3 %, and for 200 ppm SA-9 10.43 %. It can be concluded from this study that a reduction in surface tension leads to a higher nucleation site density and more small bubbles on the boiling surface.

Induction of a Hardening Phenomenon and Quantitative Changes of Ceramides in Stratum Corneum

BackgroundHardening phenomenon of human skin after repeated exposure to the irritants is well-known, but the precise mechanism remains elusive.ObjectiveTo modify the previous experimental model of hardening phenomenon by repeated applications of two different concentrations of sodium lauryl sulfate (SLS) solutions to Korean healthy volunteers and to investigate the quantitative changes of ceramides in stratum corneum before and after chronic repeated irritation.MethodsEight hundred microliters of distilled water containing 0.1% and 2% SLS was applied for 10 minutes on the forearm of 41 healthy volunteers for 3 weeks. After an intervening 3-week rest, 24-hour patch tests with 1% SLS were conducted on previously irritated sites. Transepidermal water loss (TEWL), erythema index and quantity of ceramide were measured in the stratum corneum before and after irritation.ResultsTEWL values on the sites preirritated with 2% SLS were lower than those with 0.1% SLS. Hardening phenomenon occurred in 24 volunteers at day 44. The changes in ceramide levels were not significantly higher in the hardened skin than in the non-hardened skin.ConclusionRepetitive stimulation with a higher concentration of SLS can more easily trigger skin hardening.

Acute irritant threshold correlates with barrier function, skin hydration and contact hypersensitivity in atopic dermatitis and rosacea

The aim of the study was to disclose interactions between epidermal barrier, skin irritation and sensitization in healthy and diseased skin. Transepidermal water loss (TEWL) and stratum corneum hydration (SCH) were assessed in adult patients with atopic dermatitis (AD), rosacea and healthy controls. A 4-h patch test with seven concentrations of sodium lauryl sulphate was performed to determine the irritant threshold (IT). Contact sensitization pattern was revealed by patch testing with European baseline series. Subjects with a lower IT had higher TEWL values and lower SCH. Subjects with positive allergic reactions had significantly lower IT. In AD, epidermal barrier deterioration was detected on both volar forearm and nasolabial fold, while in rosacea, impeded skin physiology parameters were observed on the facial skin only, suggesting that barrier impediment is restricted to the face in rosacea, in contrast with AD where the abnormal skin physiology is generalized.

Online conductivity and stability in the emulsion polymerization of n‐butyl methacrylate: Batch versus semibatch systems

ABSTRACTA homemade resistance probe (R) and a torroidal probe (T), which work on different principles to measure conductivity, were used as online sensors to monitor conductivity during the course of emulsion polymerizations of n‐butyl methacrylate (BMA). Six batch emulsion polymerizations of BMA (20% solids content) were carried out using sodium lauryl sulfate (SLS) as surfactant. To compare latex stability and conductivity profiles, the SLS concentration was varied (5, 6, 8, 10, 20, and 30 mM). Seven semibatch emulsion polymerizations of BMA (40% solids content) were also run. All semibatch polymerizations had the same seed stage, while different amounts of SLS were fed during the feed stage. During these reactions, the conductivity curves obtained from the two different probes overlapped if the SLS concentration was high enough (20 and 30 mM); on the other hand, the two curves diverged if the SLS concentration was low. Since the final conductivity values obtained from the two probes were not the same in most of the reactions, the ratio between them (R/T) was used to correlate conductivity to latex stability. A blender test and turbidity measurements were carried out to estimate the mechanical stability and the electrolyte stability of the prepared latexes, respectively. The results showed that R/T varied linearly with the latex stability. These results showed that latex stability could be predicted through online conductivity measurements. Moreover, the reason for an observed increase in conductivity during these emulsion polymerization reactions was also investigated. This increase was related to the disappearance of monomer droplets, which demonstrated that the conductivity measurements could also be used to aid in the study the kinetics of the emulsion polymerization process. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 4001–4013, 2013

Label-free and quantitative evaluation of cytotoxicity based on surface nanostructure and biophysical property of cells utilizing AFM

In this study, the four commonly used cytotoxicity assays and the mechanical properties as evaluated by atomic force microscopy (AFM) were compared in a cellular system. A cytotoxicity assay is the first and most essential test to evaluate biocompatibility of various toxic substances. Many of the cytotoxicity methods require complicated and labor-intensive process, as well as introduce experimental error. In addition, these methods cannot provide instantaneous and quantitative cell viability information. AFM has become an exciting analytical tool in medical, biological, and biophysical research due to its unique abilities. AFM-based force–distance curve measurements precisely measure the changes in the biophysical properties of the cell. Therefore, we observed the morphological changes and mechanical property changes in L929 cells following sodium lauryl sulfate (SLS) treatment utilizing AFM. AFM imaging showed that the toxic effects of SLS changed not only the spindle-like shape of L929 cells into a round shape, but also made a rough cell surface. As the concentration of SLS was increased, the surface roughness of L929 cell was increased, and stiffness decreased. We confirmed that inhibition of proliferation clearly increased with increases in SLS concentration based on results from MTT, WST, neutral red uptake, and LIVE/DEAD viability/cytotoxicity assays. The estimated IC50 value by AFM analysis was similar to those of other conventional assays and was included within the 95% confidence interval range. We suggest that an AFM quantitative analysis of the morphological and biophysical changes in cells can be utilized as a new method for evaluating cytotoxicity.