Slender Forms Research Articles

“Nothing Tastes as Good as Skinny Feels”

There has always been something compelling about extreme bodies. Kate Moss exemplifies “the waif,” a body type both frightening and appealing. She is one of the most common celebrities discussed on pro-anorexia (or, pro-ana) websites. To explore the connection between Moss’s body and pro-ana participation, this article examines the potential for an extraordinary body to solidify the aims of an extremist group and to draw in new members. Her unusually slender form affirms this pro-starvation cluster by physically representing the emotional distress that lies at its core. There is no conclusive evidence that Moss suffers from an eating disorder, and she has been open about discouraging people from starving themselves to look like her. Nevertheless, she has been framed as a unique icon in the pro-ana sphere, despite her personal intent, due to the extreme nature of her body. She helps members to express mental illness and severe emotional distress via an emaciated frame and grungy-but-cool aesthetic. She therefore gives people a way to explore sickness and femininity concurrently, which is especially relevant for young women with eating disorders. The pro-ana community has adopted her as a cult icon for these reasons. Here, I analyse this process using Rodney Stark and William Sims Bainbridge’s sociological scholarship on the social role of religion broadly and “cult formation” more specifically. This raises important questions about consent in cult formation and the role required of a leader.

Flabellophyton: The Benthic Macroalgal Fossils in Marwar Supergroup, India

ABSTRACT Here we report numerous fossil forms of the Ediacaran Flabellophyton genus in the Sonia Sandstone of the Jodhpur Group, Marwar Supergroup, in India. They are assigned as Flabellophyton lantianense, Flabellophyton stupendum, and Flabellophyton typicum species and have been found to have both positive and negative reliefs on the bedding planes of medium- to fine-grained sandstone in the Sursagar region of Jodhpur district. The thallus of Flabellophyton lantianensis occurs in straight to slightly curved and slender forms, is closely packed, and may have thin filaments. The Flabellophyton stupendum is a conical or fan-shaped thallus containing thin bundled filaments and has a discoidal holdfast with a well-differentiated stipe. The Flabellophyton typicum species typically has a thallus with an intermediate conical shape, a globular holdfast with an acute stipe, and a fan-shaped form with bundles of thin filaments. These studied Flabellophyton fossil assemblages are well comparable to those found in South China and South Australia in their conical or fan-shaped morphologies and holdfast structures (mostly found in F. stupendum and F. typicum species) with fine and thin bundled filaments. In accordance with this study, these Flabellophyton fossil assemblages are probably benthic macroalgal fossils. Here, the fossil assemblages of Flabellophyton from India are described for the first time.

Depolymerization of SUMO chains induces slender to stumpy differentiation in T. brucei bloodstream parasites.

Trypanosoma brucei are protozoan parasites that cause sleeping sickness in humans and nagana in cattle. Inside the mammalian host, a quorum sensing-like mechanism coordinates its differentiation from a slender replicative form into a quiescent stumpy form, limiting growth and activating metabolic pathways that are beneficial to the parasite in the insect host. The post-translational modification of proteins with the Small Ubiquitin-like MOdifier (SUMO) enables dynamic regulation of cellular metabolism. SUMO can be conjugated to its targets as a monomer but can also form oligomeric chains. Here, we have investigated the role of SUMO chains in T. brucei by abolishing the ability of SUMO to polymerize. We have found that parasites able to conjugate only SUMO monomers are primed for differentiation. This was demonstrated for monomorphic lines that are normally unable to produce stumpy forms in response to quorum sensing signaling in mice, and also for pleomorphic cell lines in which stumpy cells were observed at unusually low parasitemia levels. SUMO chain mutants showed a stumpy compatible transcriptional profile and better competence to differentiate into procyclics. Our study indicates that SUMO depolymerization may represent a coordinated signal triggered during stumpy activation program.

M6A landscape is more pervasive when Trypanosoma brucei exits the cell cycle.

N6-methyladenosine (m6A) is a mRNA modification with important roles in gene expression. In African trypanosomes, this post-transcriptional modification is detected in hundreds of transcripts and it affects the stability of the variant surface glycoprotein (VSG) transcript in the proliferating blood stream form. However, how the m6A landscape varies across the life cycle remains poorly defined. Using full-length, non-fragmented RNA, we immunoprecipitated and sequenced m6A-modified transcripts across three life cycle stages of Trypanosoma brucei - slender (proliferative), stumpy (quiescent), and procyclic forms (proliferative). We found that 1037 transcripts are methylated in at least one of these three life cycle stages. While 21% of methylated transcripts are common in the three stages of the life cycle, globally each stage has a distinct methylome. Interestingly, 47% of methylated transcripts are detected in the quiescent stumpy form only, suggesting a critical role for m6A when parasites exit the cell cycle and prepare for transmission by the Tsetse fly. In this stage, we found that a significant proportion of methylated transcripts encodes for proteins involved in RNA metabolism, which is consistent with their reduced transcription and translation. Moreover, we found that not all major surface proteins are regulated by m6A, as procyclins are not methylated, and that, within the VSG repertoire, not all VSG transcripts are demethylated upon parasite differentiation to procyclic form. This study reveals that the m6A regulatory landscape is specific to each life cycle stage, becoming more pervasive as T. brucei exits the cell cycle.

Differentiation granules, a dynamic regulator of T. brucei development.

Adaptation to a change of environment is an essential process for survival, in particular for parasitic organisms exposed to a wide range of hosts. Such adaptations include rapid control of gene expression through the formation of membraneless organelles composed of poly-A RNA and proteins. The African trypanosome Trypanosoma brucei is exquisitely sensitive to well-defined environmental stimuli that trigger cellular adaptations through differentiation events that characterise its complex life cycle. The parasite has been shown to form stress granules in vitro, and it has been proposed that such a stress response could have been repurposed to enable differentiation and facilitate parasite transmission. Therefore, we explored the composition and positional dynamics of membraneless granules formed in response to starvation stress and during differentiation in the mammalian host between the replicative slender and transmission-adapted stumpy forms. We find that T. brucei differentiation does not reflect the default response to environmental stress. Instead, the developmental response of the parasites involves a specific and programmed hierarchy of membraneless granule assembly, with distinct components and regulation by protein kinases such as TbDYRK, that are required for the parasite to successfully progress through its life cycle development and prepare for transmission.

Mitochondrion of the Trypanosoma brucei long slender bloodstream form is capable of ATP production by substrate-level phosphorylation.

The long slender bloodstream form Trypanosoma brucei maintains its essential mitochondrial membrane potential (ΔΨm) through the proton-pumping activity of the FoF1-ATP synthase operating in the reverse mode. The ATP that drives this hydrolytic reaction has long been thought to be generated by glycolysis and imported from the cytosol via an ATP/ADP carrier (AAC). Indeed, we demonstrate that AAC is the only carrier that can import ATP into the mitochondrial matrix to power the hydrolytic activity of the FoF1-ATP synthase. However, contrary to expectations, the deletion of AAC has no effect on parasite growth, virulence or levels of ΔΨm. This suggests that ATP is produced by substrate-level phosphorylation pathways in the mitochondrion. Therefore, we knocked out the succinyl-CoA synthetase (SCS) gene, a key mitochondrial enzyme that produces ATP through substrate-level phosphorylation in this parasite. Its absence resulted in changes to the metabolic landscape of the parasite, lowered virulence, and reduced mitochondrial ATP content. Strikingly, these SCS mutant parasites become more dependent on AAC as demonstrated by a 25-fold increase in their sensitivity to the AAC inhibitor, carboxyatractyloside. Since the parasites were able to adapt to the loss of SCS in culture, we also analyzed the more immediate phenotypes that manifest when SCS expression is rapidly suppressed by RNAi. Importantly, when performed under nutrient-limited conditions mimicking various host environments, SCS depletion strongly affected parasite growth and levels of ΔΨm. In totality, the data establish that the long slender bloodstream form mitochondrion is capable of generating ATP via substrate-level phosphorylation pathways.

Reaching IMO 2050 GHG Targets Exclusively Through Energy Efficiency Measures

_ Maritime transport accounts for around 3% of global anthropogenic greenhouse gas (GHG) emissions (Well-to-Wake). These GHG emissions must be reduced by at least 50% in absolute values by 2050 to contribute to the ambitions of the Paris Agreement signed in 2015. Switching to zero-carbon fuels made from renewable sources (hydro, wind, or solar) is seen by many as the most promising option to deliver the desired GHG reductions. However, renewable energy is a scarce resource that gives a much larger GHG reduction spent within other sectors. This study explores how to reach the IMO 2050 GHG targets exclusively through energy efficiency measures. The results indicate that by combining wind-assisted ship propulsion (WASP) with a slender hull form, fuel consumption and GHG emissions can be reduced by 30–35%, at a negative abatement cost for speeds exceeding 8 knots. Where the cost saving increases with the speed because at higher speeds, the fuel accounts for a higher share of the total cost, which implies that the cost saving goes from zero at 8 knots, to 5% reduction at 11 knots average speed to 14% reduction of total cost with 15 knots average speed. In comparison, GHG reductions through zero-carbon fuels will increase transport costs by 50–200%. Introduction From the first days of our civilization, sea transport has enabled regional and global trades. Today, sea transport accounts for 80% of the global trade measured in ton-miles (UNCTAD 2021) and 3% of greenhouse gas (GHG) emissions measured Well-to-Wake (Lindstad et al. 2021). More than 40% of this sea trade is performed by dry bulkers, making them the real workhorses of the sea. Even though sea transport is energy efficient compared to other transport modes, all sectors need to reduce their GHG emissions by at least 50% in absolute values by 2050 to contribute to the Paris Agreement (UNFCCC 2015). According to Bouman et al. (2017), the desired energy and GHG reductions can be achieved through: Design and other technical improvements of ships; Operational improvements; Fuels with zero or low GHG footprints; or a combination of these.

Trypanosoma lewisi in blood of Rattus rattus complex residing in human settlements, Nakhon Si Thammarat, Thailand: Microscopic and molecular investigations

Trypanosomes are blood parasites infected in various mammals, including rats. The presence of rats in human settlements can increase the chance of Trypanosoma transmission to humans. The molecular study of multispacer in Trypanosoma spp. in naturally infected rodents in Thailand is scanty. The objective of this study was to detect Trypanosoma in the blood of the captured rats in Nakhon Si Thammarat, Thailand, using microscopic and molecular techniques. This was a cross-sectional study conducted in human settlement areas. Ninety-nine blood samples were collected using cardiac puncture. A blood sample was smeared on a glass slide and examined using a compound light microscope and a scanning electron microscope. Moreover, polymerase chain reaction was applied to detect Trypanosoma evansi and T. lewisi in the blood. An additional primer set was used to confirm the species of the detected trypanosome. Approximately 18% of the rats had positive Trypanosoma infections. All Trypanosoma-positive blood samples were matched with sequences of T. lewisi. The stumpy form of trypanosome had higher nucleus related parameters than the slender form. Interestingly, the partial sequences of the alpha-tubulin gene of T. lewisi were first reported in the naturally infected RrC in this study. Based on the results obtained, T. lewisi biology, particularly the virulent components and route of transmission, pathogenesis, and in vitro experiments, are strongly recommended for further study.

Type I-like metalloproteinase in the venom of the West African saw-scaled carpet viper (Echis ocellatus) has anti-trypanosomal activity against African trypanosomes

African trypanosomiasis is an infectious disease caused by hemoparasites of the genus Trypanosoma and remains a major health problem in Africa - killing around 4000 people and animals worth an estimated $5 billion, annually. The absence of a vaccine and satisfactory drug against African trypanosomiasis (AT) necessitates the continued search for new chemotherapy options. Owing to the rich biochemical diversity in snake venom, it has recently become a source of therapeutic peptides that are being explored for the development of novel drug candidates for diverse ailments such as cancers and infectious diseases. To explore this, Echis ocellatus venom (EOV) was investigated for the presence of an anti-Trypanosoma factor, with the subsequent aim to isolate and identify it. Crude EOV was collected and tested in vitro on the bloodstream form (BSF) i.e. long and slender morphological form of Trypanosoma brucei and T. congolense. This initial testing was followed by a sequential anti-trypanosomal assay guided purification of EOV using ethanol precipitation, distillation, and ion exchange (IEX) chromatography to obtain the active trypanocidal component. The purified anti-Trypanosoma factor, estimated to be a 52-kDa protein on SDS-PAGE, was subjected to in-gel trypsin digestion and 2D RP HPLC-MS/MS to identify the protein. The anti-Trypanosoma factor was revealed to be a zinc-dependent metalloproteinase that contains the HEXXHXXGXXH adamalysin motif. This protein may provide a conceptual framework for the possible design of a safe and effective anti-trypanosomal peptide for the treatment of AT.

Effects of outrigger configuration on trimaran stability in longitudinal waves

A trimaran has excellent characteristics of transverse stability and less resistance because of its unconventional multiple slender hull form. However, the multi-hull configuration potentially triggers parametric roll due to the periodical fluctuation of metacentric height. In this study, an efficient numerical model was established to investigate the effects of the outriggers on trimaran stability in longitudinal waves, based on Froude-Krylov assumption and hydrostatic assumption. Then, the relationship between the metacentric height of the trimaran and the outrigger position was discussed, and the influence of heave/pitch motion responses on the wave stability of the trimaran was evaluated. Furthermore, a method of predicting the stability zone was proposed and checked by numerical simulation based on computational fluid dynamics. The relationship between the outrigger position and the potential risk of the parametric roll was analyzed by the obtained stability zones. It was found that the decrease in the outrigger displacement ratio and the increase in the clearance can significantly raise the risk of parametric roll of a trimaran.

Detection of Trypanosoma evansi in a naturally infected cat in Indonesia using bioassay and molecular techniques.

The prevalence of surra in domestic cat is seldom and it is caused by Trypanosoma brucei and Trypanosoma evansi. However, molecular diagnostic approaches are required owing to similarities in their morphology. In Yogyakarta, a domestic cat was diagnosed with trypanosomiasis; however, the causative species was undetermined. Therefore, we aimed to molecularly and biologically identify the isolate. Approximately 1 mL of blood from an infected cat was collected into EDTA tube and separated for inoculation into donor mice, blood smear, and DNA isolation. Two donor mice was then used for increasing the number of parasite in order to infect 10 experimental mice. Parasitemia was monitored daily in each experimental mouse by preparing a wet mount and Giemsa-stained thin blood smear. The blood of experimental mice that reached the peak of parasitemia was then collected and used for DNA isolation. Each blood sample, which collected from infected cat and experimental mice, was then isolated and amplified the DNA by polymerase chain reaction using ITS-1. The parasitemia pattern and viability of the animals were observed to determine the biological characteristics of trypanosomatid, while to assess the molecular characteristics, the internal transcribed spacer (ITS)-1 amplification was used. The prepatent period of this trypanosomatid is between 2 and 4 dpi, whereas the life span of mice is approximately 4-10 dpi. Morphologically, the trypomastigote in the cat blood smear had long slender and intermediate shapes. However, only the long slender form was detected. Among the total of 410 nucleotides (NT) of ITS-1 sequences, 25 NT substitutions differed between the cat and mouse isolates. Phylogenetic analysis revealed that both samples had a close genetic relationship with T. evansi. Trypanosoma evansi, a highly virulent trypanosomatid, was isolated from a cat in Yogyakarta.

Stability behaviour of concrete arch bridge structures in relation to the load direction out of the arch’s plane

Arches are one of the predominant structural elements for bridges with big spans. With the current technological advancement, they are applicable in pure form, without any transverse stabilizing systems. The slender form of the arch elements leads to reduction in their capacity due to stability failure. Their behaviour is directly related to the presence of first and second order effects – imperfections and deformed state. The typical bridge loads and combinations, often invoke effects outside of the elements’ plane of reference. Such conditions are reducing the capacity of the structure in relation to magnitude of the effect. Analysis of such effects is complex task including interaction between complex stress state, 3-dimensional geometry, and non-linear material behaviour. In the following work are presented series of geometrical configuration in condition of material and geometrical non-linearity for the determination of critical load leading to stability failure. The results display trends in the stability behaviour of reinforced concrete arch bridge elements.

PAG3 promotes the differentiation of bloodstream forms in Trypanosoma brucei and reveals the evolutionary relationship among the Trypanozoon trypanosomes

IntroductionTrypanosoma brucei, T. evansi and T. equiperdum are members of the subgenus Trypanozoon and are highly similar morphologically and genetically. The main differences between these three species are their differentiation patterns in the hosts and the role of vectors in their life cycles. However, the mechanisms causing these differences are still controversial.MethodsPAG3 gene was accessed by PCR amplification in 26 strains of Trypanozoon and sequences were then analyzed by BLAST accompanied with T. evansitype B group. RNA interference and CRISPR/Cas9 were used for revealing possible role of PAG3 in slender to stumpy transformation.ResultsThe procyclin associated gene 3 (PAG3) can be found in the pleomorphicspecies, T.brucei, which undergoes differentiation of slender forms to the stumpy form. This differentiation process is crucial for transmission to the tsetse fly vector. However, a homologue of PAG3 was not detected in either T. evansi or in the majority of T. equiperdum strains which are allmonomorphic. Furthere xperiments in T. brucei demonstrated that, when PAG3 was down-regulated or absent, there was a significant reduction in the differentiation from slender to stumpy forms.ConclusionTherefore, we conclude that PAG3 is a key nuclear gene involved in the slender to stumpy differentiation pathway of T.brucei in the mammalian host. Loss of this gene might also offer a simple evolutionary mechanism explaining why T. evansi and some T. equiperdum have lost the ability to differentiate and have been driven to adapt to transmission cycles that by pass the tsetse vector or mechanical contact.

Heme-deficient metabolism and impaired cellular differentiation as an evolutionary trade-off for human infectivity in Trypanosoma brucei gambiense

Resistance to African trypanosomes in humans relies in part on the high affinity targeting of a trypanosome lytic factor 1 (TLF1) to a trypanosome haptoglobin-hemoglobin receptor (HpHbR). While TLF1 avoidance by the inactivation of HpHbR contributes to Trypanosoma brucei gambiense human infectivity, the evolutionary trade-off of this adaptation is unknown, as the physiological function of the receptor remains to be elucidated. Here we show that uptake of hemoglobin via HpHbR constitutes the sole heme import pathway in the trypanosome bloodstream stage. T. b. gambiense strains carrying the inactivating mutation in HpHbR, as well as genetically engineered T. b. brucei HpHbR knock-out lines show only trace levels of intracellular heme and lack hemoprotein-based enzymatic activities, thereby providing an uncommon example of aerobic parasitic proliferation in the absence of heme. We further show that HpHbR facilitates the developmental progression from proliferating long slender forms to cell cycle-arrested stumpy forms in T. b. brucei. Accordingly, T. b. gambiense was found to be poorly competent for slender-to-stumpy differentiation unless a functional HpHbR receptor derived from T. b. brucei was genetically restored. Altogether, we identify heme-deficient metabolism and disrupted cellular differentiation as two distinct HpHbR-dependent evolutionary trade-offs for T. b. gambiense human infectivity.

Single cell and spatial transcriptomic analyses reveal microglia-plasma cell crosstalk in the brain during Trypanosoma brucei infection

Human African trypanosomiasis, or sleeping sickness, is caused by the protozoan parasite Trypanosoma brucei and induces profound reactivity of glial cells and neuroinflammation when the parasites colonise the central nervous system. However, the transcriptional and functional responses of the brain to chronic T. brucei infection remain poorly understood. By integrating single cell and spatial transcriptomics of the mouse brain, we identify that glial responses triggered by infection are readily detected in the proximity to the circumventricular organs, including the lateral and 3rd ventricle. This coincides with the spatial localisation of both slender and stumpy forms of T. brucei. Furthermore, in silico predictions and functional validations led us to identify a previously unknown crosstalk between homeostatic microglia and Cd138+ plasma cells mediated by IL-10 and B cell activating factor (BAFF) signalling. This study provides important insights and resources to improve understanding of the molecular and cellular responses in the brain during infection with African trypanosomes.

Old World Fossil Equus (Perissodactyla, Mammalia), Extant Wild Relatives and Incertae Sedis Forms

Discussion of the phylogenetic relations between Plesippus, Allohippus, and Equus. Descriptions and illustrations of 30 Equid extant and fossil species younger than 2 Ma. Particular attention is given to slender forms with short protocones usually referred to ‘Equus altidens’ from Süssenborn and Untermassfeld (Germany), Akhalkalaki and Dmanisi (Georgia), Pirro (Italy), Venta Micena (Spain) and Aïn Hanech (Algeria). Occurrence of Asinine features in fossil taxa from Africa, Greece, Mongolia, and North-Eastern Siberia. Supplementary Materials include additional discussions and photographs of fossils in particular from Süssenborn (especially those referred to E. altidens and E. marxi by Reichenau) and from Dmanisi from where a new species is described.

A Continuum Robotic Cannula With Tip Following Capability and Distal Dexterity for Intracerebral Hemorrhage Evacuation.

This paper aims to investigate a new continuum robot design and its motion implementation methods appropriate for a minimally invasive intracerebral hemorrhage (ICH) evacuation. We propose a continuum robotic cannula, consisting of a precurved body and a 2-degree-of-freedom (DoF) flexible tip, monolithically fabricated. Kinematic model with cable elongation model, and a dedicated design optimization and motion planning algorithm were developed to enable the follow-the-leader (FTL) motion of the cannula. A task-dependent Jacobian-based closed loop control was also designed to track the cannula motion during the insertion and its independent tip motion. Comprehensive experiments were conducted to verify the kinematic model and submillimeter motion coupling between the cannula precurved body and its flexible tip. The cannula was also capable of achieving FTL motion within around 2.5 mm shape deviation and control performance within submillimeter errors. It was finally demonstrated to be capable of the nonlinear insertion and tip manipulation in the brain phantom. The new cannula design, together with the proposed algorithms, provides the unique ability to access ICH in a nonlinear trajectory and dexterous tip motion. These motion capabilities of the robot in such a slender form factor will lead to more complete ICH evacuation and reduced trauma to the healthy brain tissues.

A long noncoding RNA promotes parasite differentiation in African trypanosomes.

The parasite Trypanosoma brucei causes African sleeping sickness that is fatal to patients if untreated. Parasite differentiation from a replicative slender form into a quiescent stumpy form promotes host survival and parasite transmission. Long noncoding RNAs (lncRNAs) are known to regulate cell differentiation in other eukaryotes. To determine whether lncRNAs are also involved in parasite differentiation, we used RNA sequencing to survey the T. brucei genome, identifying 1428 previously uncharacterized lncRNA genes. We find that grumpy lncRNA is a key regulator that promotes parasite differentiation into the quiescent stumpy form. This function is promoted by a small nucleolar RNA encoded within the grumpy lncRNA. snoGRUMPY binds to messenger RNAs of at least two stumpy regulatory genes, promoting their expression. grumpy overexpression reduces parasitemia in infected mice. Our analyses suggest that T. brucei lncRNAs modulate parasite-host interactions and provide a mechanism by which grumpy regulates cell differentiation in trypanosomes.

Study on Compact Pre-Swirl Duct for Slender Aft-Body Crude Oil Carrier

This study is a parametric investigation of the application of a compact type of pre-swirl duct for a slender aft-body 158k crude oil carrier. The International Maritime Organization (IMO) has created the Energy Efficiency Design Index (EEDI), which is an index related to carbon dioxide emissions, to enforce regulations on newly built ships; many solutions have been proposed, including an increase in propulsion efficiency. In the present study, a new type of energy-saving device (pre-swirl duct), the so-called ring stator, is proposed for 158k crude oil carriers, the hull form of which has been developed as a slender after-body full form to reduce the resistance by delaying separation. A conventional pre-swirl duct is no longer effective at the slender aft-body hull forms because of the small radial flow to the propeller. A parametric study was conducted through Computational Fluid Dynamics (CFD) analysis using the commercial program Star-CCM+, and an improvement of approximately 3% in the propulsion efficiency was achieved in the present study. The efficiency gain was validated through a comparison with the experimental results. The developed ring stator may become applicable to container ships through further parametric studies in the near future.

A Fully Coupled Time-Domain BEM-FEM Method for the Prediction of Symmetric Hydroelastic Responses of Ships with Forward Speed

This paper presents a direct time-domain method for the prediction of symmetric hydroelastic responses of ships progressing with forward speed in small amplitude waves. A transient time-domain free surface Green function is used for the idealisation of the seakeeping problem using an Earth fixed coordinate system. Free surface ship hydrodynamics are idealised in the time domain by a Green function, and forward speed effects are idealised by a space-state model. Modal actions are accounted for by Timoshenko beam structural dynamics. Flexible fluid structure interaction (FFSI) coupling is enabled by a body boundary condition, and a direct integration Newmark- β scheme is used to obtain symmetric dynamic responses. The method is validated against available published numerical and experimental results. A parametric study for different container ship hull forms confirms that (i) forward speed effects should be taken under consideration as far as practically possible and (ii) hull flexibility effects accounting for hull shear deformation and rotary inertia are more notable for slender hull forms.