Skin Hydration Research Articles

Background/objectives Soy isoflavones may benefit skin health in postmenopausal women, potentially via S-equol, a gut-derived metabolite with enhanced estrogenic and antioxidant activity. Methods Sixty-six postmenopausal women received either Novasoy®400 ( n = 33), a soy isoflavone extract, or identical placebo ( n = 33) for 12 weeks. Skin parameters, including crow’s feet and under-eye wrinkles, hydration, barrier function, and skin colour were assessed at baseline, D42 and D84. Urinary isoflavone metabolites, including S-equol, were measured at each time point. Results Crow’s feet wrinkle roughness decreased by 5.6% in the Novasoy®400 group versus a 1.6% increase in the placebo group, this difference was not statistically significant. However, urinary biomarker analysis identified 46.9% of women in the Novasoy®400 group and 15.6% in the placebo group as S-equol producers. In exploratory regression analysis limited to S-equol producers, higher urinary S-equol levels were significantly associated with improved under-eye wrinkle parameters and transepidermal water loss (TEWL) at D42. Multivariable model adjusting for age, soy intake, phototype and their interactions confirmed that older women experienced greater improvements in undereye wrinkles and barrier function in response to increased S-equol levels. Skin hydration showed a modest but statistically significant association with S-equol when combining data from both D42&84, indicating a potential cumulative effect. Conclusion Soy isoflavone supplementation increased urinary S-equol concentrations and was associated with improvements in under-eye wrinkle appearance, skin hydration, and barrier function—particularly among older postmenopausal women identified as S-equol producers. Findings suggest region-specific, time-sensitive effects dependent on S-equol levels. Clinical trial registration https://clinicaltrials.gov/study/NCT06047145 , NCT06047145.

Skin temperature adapted physiological strain index (aPSI) predicts exertional heat illness

Skin is the largest organ of the human body which is affected by aging. The objective of this review paper is to briefly highlights the factors responsible for skin changes in aging and the role of nutraceutical synergies in counteracting the signs of aging. Ageing is a multifaceted biological process impacting skin health through intrinsic genetic programming and extrinsic environmental factors. Intrinsic ageing, characterized by hormonal changes and fibroblast dysfunction, and extrinsic factors, such as reactive oxygen species and advanced glycation end-products, compromise skin structure and function. Modern lifestyles, including poor nutrition, environmental pollutants, and UV exposure exacerbate oxidative stress and accelerate skin ageing. Nutraceutical interventions demonstrate significant potential in mitigating these effects, including collagen peptides, hyaluronic acid, nicotinamide mononucleotide, and trace elements like zinc and copper. These bioactive compounds promote collagen synthesis, enhance hydration, and reduce oxidative stress, improving skin elasticity, hydration, and resilience. This study emphasizes the efficacy of a multi-nutraceutical approach, which synergistically addresses ageing pathways, providing a promising avenue for personalized, effective anti-ageing strategies. Future research is recommended to focus on long-term, populationdiverse studies to validate these findings and expand their applicability. BJMS, Vol. 24 No. 04 October’25 Page : 1072-1078

Objective To prepare low-temperature extracted Portulaca oleracea L. (P. oleracea) and investigate the application of masks containing this extract in photorejuvenation for sensitive skin. Methods Eligible participants were randomly divided into two groups. Experimental group used the mask for 14 days prior to photorejuvenation. Both groups used the mask for 7 days post-treatment. Changes in physiological parameters and Dermatology Life Quality Index (DLQI) were observed. Results Immediately after photorejuvenation, control group showed a significant increase in skin red area and red blood cell concentration, while experimental group did not. At 7 days post-treatment, skin hydration and transepidermal water loss (TEWL) improved significantly in both groups, with greater improvement in the experimental group. Both groups’ DLQI decreased, with a significant reduction in the experimental group. Conclusion Facial masks containing low-temperature extracted P. oleracea show promising potential in protecting sensitive skin from photothermal stimulation and improving skin barrier function during photorejuvenation therapy.

Mycosporine-like amino acids (MAAs) are multifunctional, UV-absorbing and antioxidant metabolites produced by marine algae, offering promising applications in biotechnology and dermocosmetic sciences. In this study, MAAs were sustainably extracted from nori seaweed (Porphyra spp.) using an ultrasound-assisted aqueous method, an eco-friendly approach that ensures efficiency and industrial scalability. Chromatographic enrichment followed by MALDI-TOF mass spectrometry confirmed the presence of bioactive compounds, including porphyra-334, palythine, and myc-ornithine. The enriched fraction exhibited potent antioxidant activity (low IC50 in DPPH and ABTS assays) and significant anti-elastase effects, highlighting its potential as a natural anti-aging agent. To optimize delivery, MAAs were incorporated into a stable water-in-oil nanoemulsion, which maintained droplet sizes below 400 nm and a low polydispersity index (PDI < 0.2) for up to four months. A randomized, double-blind clinical study in 20 volunteers further demonstrated that the MAA-based nanoemulsion significantly improved skin hydration (+53.6%) and reduced transepidermal water loss (TEWL), confirming its humectant and barrier-strengthening efficacy. These findings position Porphyra spp. as a sustainable marine resource for producing MAAs, and demonstrate their practical potential as natural, multifunctional ingredients in eco-conscious cosmetic and pharmaceutical formulations.

Topically applied hyaluronic acid (HA) hydrates the skin without efficient penetration. This study compared the penetration efficiency of liposomal hyaluronic acid (LPS-HA) against that of conventional HA across different models. Dynamic light scattering revealed that particles of LPS-HA (226.1 nm, PDI 0.2898) were smaller than those of HA (798.4 nm, PDI 0.8709). In Strat-M® membrane assays, permeability over 24 h was higher with LPS-HA (629.37 ± 103.26%) than that of HA (508.04 ± 93.80%; p<0.05). In keratinocytes, LPS-HA increased differentiation markers filaggrin and caspase-14 in a concentration-dependent manner, with maximal induction at 1% (186.6 ± 6.99% and 249.3 ± 8.60%) vs. HA (117.9 ± 7.64% and 130.1 ± 2.90%; p<0.05). In fibroblasts, LPS-HA increased the expression of type I and type III collagens (138.4 and 133.6%) without increasing that of elastin (68.3–94.7%) and reduced UVB-induced IL-6 (79.1–90.2% of UVB; p<0.05). Ex vivo, LPS-HA enhanced HAS-3 mRNA (3.03 ± 0.19-fold vs. 1.31 ± 0.13-fold with HA; p<0.05) and increased epidermal hyaluronan staining. In PM10-treated human skin, LPS-HA reduced inflammatory cytokines (TNF-α, IL-6, IL-8, and IL-1β) and suppressed mast cell degranulation, similar to dexamethasone, and reduced ROS formation (124.46 ± 8.45% vs. 169.35 ± 9.40% in PM10-only, p<0.01) without histological abnormalities. In a 20-subject clinical study, corneometric hydration with LPS-HA was higher than that of control (96.99% vs. 36.31%; RM-ANOVA, p<0.001). Collectively, LPS-HA enhanced skin permeation, hydration, and anti-inflammatory responses, supporting its potential as a cosmetic moisturizing ingredient.

Skin compatibility is a common issue and can often be worsened by certain ingredients in cosmetics. This is why developing well-balanced and -tolerated formulas is now an essential challenge. In this work we developed a cream rich in antioxidant, soothing, and moisturizing agents complying with concentration limits for sensitive skin. An initial optimization was carried out, and the best-performing formula was fully characterized to test its rheological properties under static or dynamic conditions and product safety. The formulation proved to be highly stable even under thermal stress, as shown by Turbiscan Lab analyses, which reported backscattering values ±2. Rheological tests also indicated a solid-like behavior with reduced viscosity at skin temperature of 32 °C, confirming the good spreadability of the cream. Finally, in vivo tests on healthy volunteers showed excellent safety results and good overall appreciation of the product. No changes in transepidermal water loss (7.9 ± 3.5 vs. 5.5 ± 0.4, p &gt; 0.05), skin hydration (44.2 ± 18.6 vs. 50.5 ± 14.1, p &gt; 0.05), or color were detected within 6 h from application, compared with baseline values. Moreover, volunteers highlighted the cream’s suitability for dry skin and expressed satisfaction with spreadability, a nourishing and hydrating sensation after application, and the absence of residues, consistently rating them ≥4 in the skin feeling questionnaire. These results are promising and support the potential use of the product on sensitive skin.

Porcine placenta peptides as a complementary functional food for skin rejuvenation: A 12-week randomized, double-blind, placebo-controlled trial.

Abstract Background Psoriasis, eczema, fungal infections, and skin aging are prevalent dermatological conditions characterized by chronic inflammation, impaired skin barrier function, and a reduced quality of life. Conventional topical cosmetic treatments, however, are constrained by limited suboptimal efficacy, instability of active compounds, and inadequate skin penetration. The application of nanotechnology in cosmetics, known as nanocosmeceuticals, has emerged as a promising strategy to overcome these drawbacks. Lipid-based nanocarriers, including liposomes, phytosomes, niosomes, and solid lipid nanoparticles, facilitate site-specific delivery, enhance transdermal permeation, and protect bioactive constituents from degradation, improving therapeutic effectiveness. Main body This study aims to provide a comprehensive evaluation of lipid-based nanocosmeceuticals for the prevention and treatment of skin aging and related dermatological disorders, with particular emphasis on formulation strategies, therapeutic efficacy, and safety considerations. This work presents a comprehensive review of peer-reviewed studies addressing the physicochemical properties, delivery strategies, and both in vitro and in vivo performance of lipid-based nanocarriers in dermatological applications. Studies show lipid-based nanocarriers exhibit minimal toxicity, enhanced stability, and controlled, sustained release profiles, with the added ability to deliver active compounds to targeted skin sites. Reported benefits include improved skin hydration, reduced wrinkles, and more effective management of psoriasis, eczema, and microbial infections. Their high entrapment efficiency and capacity for co-delivery of multiple drugs further strengthen their potential as versatile platforms for dermatological therapy. Conclusion Lipid-based nanocosmeceuticals represent novel and versatile platforms that provide both cosmetic and therapeutic benefits. They hold strong potential to transform dermatological care through enhanced efficacy and improved safety; however, large-scale clinical trials and regulatory harmonization remain essential to enable their successful translation into clinical practice. Graphical abstract

Acne vulgaris is a prevalent chronic inflammatory skin condition driven by excessive sebum secretion and follicular blockage. While most existing acne patches emphasize downstream effects, such as antibacterial and anti-inflammatory effects, they neglect sebum accumulation, a key upstream factor. Efficient removal of this lipid-rich biofluid remains a major challenge. Although existing unidirectional drainage systems have shown success in managing aqueous wound exudates through capillary-driven flow, their designs are poorly suited for viscous, nonpolar secretions like sebum. Here, we present a Janus interpenetrating dressing (BJID) consisting of an oleophilic absorbing layer/underwater oleophobic barrier layer to form a structurally interlocked interface. Mimicking natural liquid collection and directional self-transport mechanisms, the BJID achieves unidirectional oil-phase sebum drainage while preventing backflow. Taking advantage of conical micropores, anisotropic wettability, and directional capillary forces, BJID enables a high rectification ratio of 4.4 and directional transport of sebum droplet while inhibiting reverse diffusion. It also reduces intracellular ROS and suppresses inflammation via the TLR2/NF-κB pathway in C. acnes-stimulated HaCaT cells. In a three-week human trial, BJID reduced acne size and sebum by 31.8% and 21.3% and increased skin hydration by 13.1%. These findings highlight the potential of bioinspired unidirectional oil-phase drainage materials as a new strategy for regulating skin microenvironments and improving acne therapy.

The perceived age and actual chronological age of a person can vary widely as a result of age-related features, such as wrinkles or hyperpigmented spots, that affect overall appearance. The perceived age of an individual can differ significantly from their chronological age, depending on environmental or lifestyle factors. While age-related changes of the skin microbiome have been characterized in healthy older and younger adults, the inter-relationship between the skin microbiome and the physiological appearance of ageing remains to be elucidated. To compare the skin microbiome features of healthy adult women of similar chronological age (40-50 years old) exhibiting distinct premature and delayed ageing of the skin. Participants exhibiting premature and delayed skin ageing were defined based on historical VISIA CR® images. Thirty volunteers with signs of premature ageing and 35 with delayed skin ageing were recruited. Participants with premature skin ageing were given a marketed product containing retinyl propionate to use twice daily for 4 weeks. Skin brightness, elasticity, hydration and barrier function were measured at baseline and 4 weeks after product use. Facial skin microbiome samples were collected from participants' upper cheeks using a cup scrub technique. Microbial DNA was extracted and sequenced at the variable region (V1-V2) of the 16S rDNA gene for bacterial classification and analysis. We found significant changes in the skin microbiome between participants with premature skin ageing and those with delayed skin ageing, with an increased abundance of Acinetobacter in those with premature skin ageing. Using network theory to characterize polymicrobial interactions, we found that the microbial community network in skin showing signs of premature ageing was more fragile and less resilient than that of skin showing delayed ageing. Topical use of a marketed skincare product containing retinyl propionate could improve age-related skin physiology in people with premature skin ageing while modulating the microbiome's composition and community structure toward that found in people with delayed skin ageing. Our findings unravel the potential role of the skin microbiome in the appearance of premature skin ageing and lay the foundation for the future development of a microbiome-based antiageing intervention strategy.

We investigated skin hydration and anti-ageing efficacies of Ectoine, a natural bacterial osmolyte, utilising human keratinocyte (HaCaT) and fibroblast (Hs68) cells under non- or UVB (30 mJ/cm2) exposure. We incorporated various experimental techniques, including MTT assay (cell viability), small interfering RNA (siRNA) transfection, and immunoblotting analysis, to assess the molecular mechanisms of skin hydration and anti-ageing efficacies of Ectoine. The findings exhibited that Ectoine improved cell viability in the presence or absence of UVB exposure in HaCaT cells. Ectoine upregulated Src and HAS-2 expression in the presence or absence of UVB irradiation in HaCaT cells. Src knockdown reduced Ectoine-increased HAS-2 expression, implying that Ectoine provoked Src-mediated HAS-2 expression in the presence of or without UVB irradiation in HaCaT cells. Ectoine enhanced phosphorylated-ERK expression in a time- and dose-dependent manner in HaCaT cells. In addition, Ectoine increased phosphorylated-ERK expression in UVB-irradiated HaCaT cells. The ERK inhibitor (PD98059) remarkably decreased Ectoine-provoked HAS-2 expression, suggesting that Ectoine triggered ERK-mediated HAS-2 expression in HaCaT cells. Furthermore, Ectoine amplified JNK and AQP-3 levels in the presence or lack of UVB exposure in HaCaT cells. The JNK inhibitor (SP600125) significantly reduced Ectoine-triggered AQP-3 expression, suggesting that Ectoine provoked JNK-mediated AQP-3 expression in HaCaT cells. Ectoine increased collagen-I expression in the presence or lack of UVB irradiation in HaCaT cells. Notably, Ectoine enhanced collagen-I expression and inhibited MMP-1 expression in a dose-dependent manner in fibroblast (Hs68) cells. We demonstrated that Ectoine exerts skin hydration effects without or with UVB exposure in human skin keratinocyte (HaCaT) cells and anti-ageing in fibroblast (Hs68) cells. Therefore, Ectoine could serve as a potential natural compound in cosmetic preparations for skin hydration and anti-ageing.

This pilot, proof-of-concept, study was designed to assess the effect of a blend of Lactobacillus helveticus Rosell®-52 and Bifidobacterium longum Rosell®-175 on parameters of skin quality. Capsules containing L. helveticus Rosell®-52 and B. longum Rosell®-175 were given to 35 healthy women (3 billion CFU/d for 56 days), and assessments of skin quality were performed at baseline, as well as on days 28 and 56. Importantly, the formulation significantly reduced the appearance of fine lines and improved skin firmness, elasticity, and hydration. In addition, study participants experienced improvements in sleep quality and felt less stressed (PSS-10 subjective stress questionnaire). Taken together, these results provide the rationale to conduct a larger, placebo-controlled trial and to assess the effects of the oral probiotic intake in healthy women with more visible signs of skin aging.

ABSTRACTSKH‐1 hairless mice were used as the in vivo model in this study to evaluate the efficacy of oral snail extract powder (SEP) on skin hydration and barrier enhancement. The skin serves as a primary barrier against external stimuli and plays a critical role in maintaining water homeostasis. Optimal skin hydration is essential for preserving barrier integrity and preventing dermatological conditions such as xerosis and atopic dermatitis. Although edible snail has demonstrated moisturizing effects when applied topically, its efficacy following oral administration has not been sufficiently characterized. In this study, we evaluated the moisturizing efficacy and safety of orally administered edible snail extract powder in SKH‐1 hairless mice. Animals were divided into a control group and three treatment groups receiving 20, 40, or 80 mg/kg B.W. of SEP daily for 13 weeks. Skin hydration and transepidermal water loss (TEWL) were measured periodically throughout the experiment. To investigate the underlying mechanisms, we analyzed the mRNA expression of genes related to hydration and barrier function, quantified skin hyaluronic acid (HA) levels via ELISA, and performed immunohistochemical staining. Oral SEP administration increased skin hydration and decreased TEWL, suggesting improved barrier function. Gene expression analyses revealed upregulation of Has1–3, Col1a1, Col3a1, Tgf‐β1, Flg, and Cers2, along with downregulation of Hyal1 and Acer1. These effects were supported by higher hyaluronic acid content and confirmed by altered protein expression of HAS2, HYAL1, and TGF‐β1. No significant changes were observed in body weight, food intake, or organ weights, and no adverse effects were observed at tested doses, supporting the safety of oral SEP administration. Overall, these findings suggest that orally administered SEP improves skin hydration and strengthens barrier integrity by regulating both molecular and tissue‐level pathways, highlighting its potential as a functional food ingredient for skin health.

Atrophic scars, caused by insufficient collagen deposition and ECM production during wound healing, significantly affect patients' quality of life. Conventional treatments often fail to achieve complete skin regeneration, prompting interest in regenerative medicine. This review evaluates the efficacy and safety of emerging regenerative therapies, including extracellular vesicles, conditioned media, stromal vascular fraction, and mesenchymal stem cells, in improving atrophic scars. A systematic search was conducted on PubMed, Scopus, and Web of Science up to November 23, 2024. Relevant keywords, including "conditioned media," "Secretome," "extracellular vesicles," "atrophic scar," "rejuvenation," and "lightening," were used to identify studies. Articles meeting inclusion criteria underwent data extraction focusing on study design, population characteristics (mean and range), intervention details (including duration), comparison groups, clinical outcomes with statistical results, and reported adverse effects, ensuring comprehensive analysis and adherence to PRISMA guidelines. Out of 186 initially identified articles, 11 studies involving 177 participants (89 females, 59 males, and 29 undefined, mean age 31.52 years) were reviewed, focusing on treatments for acne scars (82%) and striae distensae (18%). These studies evaluated stromal vascular fraction (SVF), cell-conditioned medium (CM), mesenchymal stem cells (MSC), and extracellular vesicles (EV). Significant improvements were observed with various treatments, including a 32.5% decrease in ECCA scores with adipose tissue stem cell exosome (ASCE) treatment, and greater acne scar improvements with Fractional CO2 Laser combined with platelet-rich plasma compared to stem cell-conditioned medium (SC-CM). Other promising results included enhanced skin elasticity and collagen density with ADSC-CM and a 28.5% reduction in scar volume with HSCM combined with FCL. SVF treatments, including gel and Subcision, demonstrated notable improvements in striae distensae and acne scars, with significant reductions in scar volume, area, and depth. Intradermal bone marrow stem cell injections also led to significant acne scar improvements, highlighting the potential of stem cell-based and exosome treatments for skin regeneration. The evaluated studies indicate promising efficacy for regenerative treatments such as SVF, CM, EV, and MSC in improving acne scars and striae distensae. Significant enhancements in scar appearance, skin hydration, and patient satisfaction were observed across various combination therapies compared to controls. While these advancements offer new hope for patients with moderate to severe skin conditions, further research is needed to establish standardized protocols and long-term efficacy. The minimal reported adverse effects highlight the safety of these regenerative interventions, suggesting that their integration into dermatological practice could improve treatment outcomes for skin texture and appearance. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Monitoring skin health through parameters like skin hydration (SH) and transepidermal water loss (TEWL) is vital for diagnosing skin conditions and identifying disease factors. Conventional devices and survey-based methods often fail to deliver accurate diagnoses due to circadian rhythms of skin health data, limited measurement frequency, and patient subjectivity. Previous research has shown that prolonged device usage also causes sweat accumulation, compromising reliable monitoring. Here, we present a breathable skin health analyzer (BSA), a wearable device designed for prolonged use, capable of accurate, long-term measurement of SH and TEWL. The BSA addresses considerable obstacles in skin health monitoring by employing a breathable chamber and a bistable actuator that ensures both ventilation and consistent sensor contact with the skin. Validated through a 28-day clinical trial, the BSA and data processing algorithms demonstrated their effectiveness in providing reliable data by analyzing the correlation between particulate matter exposure and the skin barrier health. These results not only highlight the potential to improve the diagnosis and treatment of diseases but also show the possibility of contributing to individual environmental health impact assessments and translational studies.

Balneotherapy has traditionally been associated with skin health and psychological well-being. However, few studies have assessed its effects during extended use in real-world, community-based settings.This study aimed to evaluate the effects of repeated balneotherapy on skin barrier function and psychological stress regulation in middle-aged women.A 16-week quasi-experimental trial was conducted in Asan, Republic of Korea, involving 58 community-dwelling women aged 40-64 years. Participants were assigned to either an intervention group (n = 29), which received biweekly 20-minute immersion sessions in naturally mineralized hot spring water, or a control group (n = 29) with no spa exposure. Primary outcomes included corneometry-based skin hydration and transepidermal water loss (TEWL). Secondary outcomes were assessed using the Stress Response Inventory (SRI), salivary cortisol, heart rate variability (HRV), and the Patient Global Impression of Change (PGIC).Significant in-group improvements were observed in the intervention group for skin hydration (p < 0.001), TEWL (p < 0.001), and SRI scores (p = 0.043). Between-group comparisons at week 16 showed significant differences for skin hydration, TEWL, SRI, and PGIC (all p < 0.05), whereas no significant differences were found for salivary cortisol or HRV.Repeated balneotherapy over 16 weeks improved skin barrier function and reduced psychological stress in middle-aged women. These findings support the feasibility of thermal bathing as a non-pharmacologic, community-based intervention for preventive wellness and highlight the need for larger randomized trials with long-term follow-up.

Aging leads to structural and compositional changes in the skin, contributing to more wrinkles, less smoothness, and dryness. There is growing interest in oral non-invasive interventions to help ameliorate visual signs of skin aging. The purpose of this study was to evaluate the efficacy of an oral skincare supplement containing hyaluronic acid and wheat oil extract on parameters of skin aging in a diverse population. Healthy adults (ages 26-64) were recruited and randomly assigned to the intervention group (n = 31) or placebo group (n = 32). The intervention group received an oral skincare supplement consisting of wheat oil extract and low molecular weight sodium hyaluronate, with a 12-week follow-up period. The intervention group demonstrated a significant reduction in crow's feet wrinkles and improvements in both facial and global skin smoothness beginning at week8 and continuing to week12, compared to the placebo group. Additionally, the intervention group exhibited significant increases in skin hydration and elasticity in the leg region relative to the placebo group. Dermatologists' evaluations revealed significant reductions in global scores of both deep and fine wrinkles from baseline to week12, but only in the intervention group. By week12, a greater proportion of participants in the intervention group reported improvements in fine lines and wrinkles, skin radiance/glow, skin resilience/elasticity, and overall signs of aging, with these changes being significantly different from the placebo group. An oral skincare supplement combining wheat oil extract and hyaluronic acid offers a comprehensive method for addressing skin aging, providing measurable clinical improvements in various skin parameters and enhancing subjective perceptions of skin health and appearance. ClinicalTrials.gov identifier, NCT06083402.

Botanical-based lip balm for enhanced skin hydration and antioxidant defense: a green chemistry approach

Background: Contemporary dermatology and cosmetology continue to explore effective strategies for normalizing the function of oily skin, where excessive sebum production and impairment of the hydrolipid barrier pose considerable therapeutic challenges. The aim of the present study was to evaluate the effects of a series of microneedling mesotherapy treatments with a sebum-regulating ampoule on selected biophysical parameters of the skin in individuals with oily skin. Methods: The study included 19 female volunteers aged 18–42 years, who underwent six treatment sessions at three-week intervals. Skin parameters were assessed at baseline, after three sessions, and after six sessions using the MPA system (Courage &amp; Khazaka) equipped with the following probes: Corneometer (hydration), Sebumeter (sebum secretion), pH meter (surface pH), Glossymeter (skin shininess), and Tewameter (transepidermal water loss). Results: After six sessions, hydration significantly increased both in the T-zone (from 43.9 ± 8.0 to 54.0 ± 5.4 AU; +23%) and on the cheeks (from 35.9 ± 8.3 to 55.6 ± 4.8 AU; +55%) (p &lt; 0.001). Sebum secretion decreased markedly, with values in the T-zone falling from 192.2 ± 30.6 to 127.7 ± 27.2 AU (−34%) and on the cheeks from 185.0 ± 36.2 to 114.8 ± 30.1 AU (−38%) (p &lt; 0.001). Skin surface pH showed minor but significant modulation within the physiological range (T-zone: 6.33 ± 0.64 → 6.01 ± 0.17; cheeks: 6.14 ± 0.50 → 6.03 ± 0.17; p = 0.021). TEWL demonstrated a nonsignificant change (T-zone: 17.46 ± 11.31 → 19.09 ± 3.54 g/m2/h; cheeks: 20.89 ± 5.36 → 18.37 ± 2.95 g/m2/h; p &gt; 0.05), while skin gloss remained stable (T-zone: 5.46 ± 1.25 → 5.60 ± 1.16 GU; cheeks: 5.29 ± 1.76 → 4.87 ± 1.20 GU; p &gt; 0.05). Conclusions: Microneedling mesotherapy combined with a sebum-regulating ampoule significantly improved skin hydration and reduced sebum secretion, accompanied by stabilization of skin surface pH. Although changes in TEWL and gloss were not statistically significant, the overall results indicate improved skin condition and balance. Despite the absence of a control group, these findings support the potential of this combined approach as an adjunctive therapy for oily skin. Further controlled studies with larger cohorts are warranted to confirm its efficacy and long-term effects.