Size Sorting Research Articles

Grain size characteristics of surface sediment in the Jilantai Salt Lake Protection System after long‐term wind‐sand activities

AbstractIn order to explore the influence of vegetation on homogeneity of surface particle size distribution (PSD) and heterogeneity of soil structure, observations, theory, and multifractal results were synthesized to highlight the evolution of the soil particle composition in the Jilantai Salt Lake Protection System (Jilantai system) after long‐term wind‐sand activities and its soil structure characteristics. From flowing sand dune to saline‐alkali wasteland areas, the mean grain size and surface particle sorting degree gradually increased and decreased, respectively, whereas the shape of the particle frequency curve gradually changed from extremely positive deviation to negative deviation, and the peak shape of the curve gradually changed from narrow to wide. Furthermore, the inhomogeneity of the soil particle composition gradually decreased, whereas the range of the soil PSD gradually increased, and the soil increasingly became fine‐grained. Therefore, the contents of clay and silt in shelterbelt soil increased by 16% and 18.36%, respectively, over 1986 levels. Additionally, clay and silt play a vital role in determining soil particle parameters such as standard deviation (SD), skewness (SKI), and monofractal (D), while vegetation factors are the main controlling factors of clay and silt. In conclusion, the Jilantai system could increase heterogeneity and homogeneity and decrease the concentration of soil PSD by changing the sedimentary environment, while this affect depends largely on the fractional vegetation cover, vegetation height, and light penetrating porosity. Moreover, the multifractal and particle parameters could describe the soil structure characteristics and sedimentary process, respectively, after long‐term wind‐sand activities.

Surface Acoustic Wave-Based Microfluidic Device for Microparticles Manipulation: Effects of Microchannel Elasticity on the Device Performance.

Size sorting, line focusing, and isolation of microparticles or cells are fundamental ingredients in the improvement of disease diagnostic tools adopted in biology and biomedicine. Microfluidic devices are exploited as a solution to transport and manipulate (bio)particles via a liquid flow. Use of acoustic waves traveling through the fluid provides non-contact solutions to the handling goal, by exploiting the acoustophoretic phenomenon. In this paper, a finite element model of a microfluidic surface acoustic wave-based device for the manipulation of microparticles is reported. Counter-propagating waves are designed to interfere inside a PDMS microchannel and generate a standing surface acoustic wave which is transmitted to the fluid as a standing pressure field. A model of the cross-section of the device is considered to perform a sensitivity analysis of such a standing pressure field to uncertainties related to the geometry of the microchannel, especially in terms of thickness and width of the fluid domain. To also assess the effects caused by possible secondary waves traveling in the microchannel, the PDMS is modeled as an elastic solid material. Remarkable effects and possible issues in microparticle actuation, as related to the size of the microchannel, are discussed by way of exemplary results.

Sedimentary Characteristics and Hydrocarbon-Generation Potential of the Permian Pingdiquan Formation in Dongdaohaizi Sag, Junggar Basin, Northwest China.

After several years of extensive exploration, a series of oil and gas reservoirs or structures have been discovered in the Dongdaohaizi sag, situated in the hinterland of the Junggar Basin. However, in recent years, some wells around the sag have failed. Herein, sedimentary facies, the plane distribution of dark mudstone, geochemical characteristics of source rocks, and the control of sedimentary facies on the distribution and development of source rocks in the Permian Pingdiquan Formation in the Dongdaohaizi sag were systematically investigated. A combined geological and geochemical method was used to fully understand the distribution law and resource potential of the source rocks in this area. Investigation results indicate that the Pingdiquan Formation primarily inherited continuous deposition, and the sedimentary facies of the sag, such as semideep lake, shore-shallow lake, prefan delta, fan delta front, and fan delta plain, were developed from the center sag to the outside. The fan delta facies, predominantly distributed in the northern margin of the sag, are characterized by lithology mainly composed of sandy conglomerate and coarse sandstone. The sandstone has a coarse particle size and poor sorting ability, while lacustrine mudstone is widely developed in the sag with a large sedimentary thickness, which is the primary source rock of the Dongdaohaizi sag. The source rocks in the Pingdiquan Formation exhibit medium-to-good quality. The organic matter is categorized as types II1-III, and the thermal evolution maturity is in the mature-high mature stage, indicating a high resource potential. The development of the source rock in the Dongdaohaizi sag is closely related to the sedimentary environment. The sedimentation rate of fan delta facies is high and burial is fast, bringing a large amount of terrestrial organic matter to the lake, thereby increasing the nutrition and organic matter quantity of the lake. Due to the stable water of the lake, low energy at the bottom, and medium conditions of strong reduction, a large amount of organic matter accumulated in the deep to semideep lake facies of the study area, resulting in the development of thick dark mudstone with a thickness of over 400 m. The abundance of organic matter has reached a good level, and the maturity and content of organic matter are high, signifying a high hydrocarbon-generation potential.

Precise size sorting of nanoparticles by bound states in the continuum in a dual finite grating.

We consider two parallel dielectric gratings (dual grating) which support accidental bound states in the continuum (BICs) mostly localized between gratings. As distinctive to true periodical BICs in an infinite dual grating, the enveloping intensity of quasi-BICs in a finite dual grating behaves as a standing wave. That behavior is a key property to trap nanoparticles into selected cells of the dual grating sorted by sizes of nanoparticles dragged by liquid flowing between gratings. For excitation of quasi-BIC with high quality factor by an electromagnetic plane wave with normal incidence and power 1 mW/µm2 we show high efficiency of sorting of nanoparticles by sizes.

Rapid and Accurate Crayfish Sorting by Size and Maturity Based on Improved YOLOv5

In response to the issues of high-intensity labor, low efficiency, and potential damage to crayfish associated with traditional manual sorting methods, an automated and non-contact sorting approach based on an improved YOLOv5 algorithm is proposed for the rapid sorting of crayfish maturity and size. To address the difficulty in focusing on small crayfish, the Backbone is augmented with Coordinate Attention to boost its capability to extract features. Additionally, to address the difficulty in achieving high overall algorithm efficiency and reducing feature redundancy, the Bottleneck Transformer is integrated into both the Backbone and Neck, which improves the accuracy, generalization performance, and the model’s computational proficiency. The dataset of 3464 images of crayfish collected from a crayfish breeding farm is used for the experiments. The dataset is partitioned randomly, with 80% of the data used for training and the remaining 20% used for testing. The results indicate that the proposed algorithm achieves an mAP of 98.8%. Finally, the model is deployed using TensorRT, and the processing time for an image is reduced to just 2 ms, which greatly improves the processing speed of the model. In conclusion, this approach provides an accurate, efficient, fast, and automated solution for crayfish sorting.

Investigating the Relationship between Polarimetric Radar Signatures of Hydrometeor Size Sorting and Tornadic Potential in Simulated Supercells

Abstract The national upgrade of the operational weather radar network to include polarimetric capabilities has led to numerous studies focusing on polarimetric radar signatures commonly observed in supercells. One such signature is the horizontal separation of regions of enhanced differential reflectivity (ZDR) and specific differential phase (KDP) values due to hydrometeor size sorting. Recent observational studies have shown that the orientation of this separation tends to be more perpendicular to storm motion in supercells that produce tornadoes. Although this finding has potential operational utility, the physical relationship between this observed radar signature and tornadic potential is not known. This study uses an ensemble of supercell simulations initialized with tornadic and nontornadic environments to investigate this connection. The tendency for tornadic supercells to have a more perpendicular separation orientation was reproduced, although to a lesser degree. This difference in orientation angles was caused by stronger rearward storm-relative flow in the nontornadic supercells, leading to a rearward shift of precipitation and, therefore, the enhanced KDP region within the supercell. Further, this resulted in an unfavorable rearward shift of the negative buoyancy region, which led to an order of magnitude less baroclinic generation of circulation in the nontornadic simulations compared to tornadic simulations.

Quantitative reconstruction of competing sources reveals spatial heterogeneity and complex aeolian–fluvial interactions in the Horqin Sandy Land, NE China

AbstractThe provenance and aeolian–fluvial interaction are essential for understanding the formation and evolution of sandy land. However, the dune sediment source in the Horqin Sandy Land (HQSL), the largest semi‐stable sandy land in China, has been debated. Here, we collected 900 coarse (i.e., >63 μm) and 750 fine (i.e., <63 μm) detrital zircon (DZ) grains from surface sand in different spatial locations for U–Pb age analysis. The visual comparison largely shows remarkably similar zircon age characteristics throughout the HQSL, and the quantitative results of the inverse Monte Carlo modelling show the primary detrital contribution of the Central Asian orogenic belt (~50.5%–61.3%) to the HQSL. In contrast, the southeast of the HQSL is characterized by the absolute dominance of the North China Craton (~75.8%). The DZ age spectra in the western and northern HQSL are extremely similar but significantly different from the south, indicating the spatial heterogeneity of the HQSL. The DZ ages are largely unaffected by particle size sorting, except in the southeastern HQSL (e.g., the dramatically different detrital contribution of the North China Craton to the fine [52.4%] and coarse fractions [75.8%]). We argue that the aeolian–fluvial interaction and the resulting sedimentary sorting and recycling are responsible for the DZ age characteristics of the HQSL. We confirm that the DZ ages of the HQSL are a faithful record of the tectonic‐magmatic events, for example, the North China Craton massif collision and collusion, the Paleo‐Asian Ocean subduction and closure, the Mongolian‐Okhotsk Ocean plate subduction and the paleo‐Pacific Ocean subduction and retreat. Finally, this study suggests that caution must be exercised in interpreting the paleoclimatic record and the aeolian deposit source investigations due to the spatial heterogeneity and the complex wind‐fluvial interactions in the HQSL.

Utilizing Geochemical Analysis for Provenance Determination in Sedimentary Systems

Geochemical and isotopic analysis represents a potent approach for investigating sediment provenance, a crucial aspect for paleogeographic reconstructions, understanding the evolution of the Earth's crust and characterizing geology in areas that are no longer exposed at the surface. The sediment composition can be influenced by multiple processes including the weathering and erosion of the source rock, mixing with other sediment sources, grain size sorting and hydrodynamic processes such as water or wind transport. Additionally, climate and topography can impact the sediment composition by affecting the rate and nature of weathering and erosion as well as the transport and deposition of sediments. Moreover, post-depositional processes such as diagenesis and metamorphism have the potential to modify the original isotopic and geochemical composition of sediments. The objective of the present investigation is to conduct an overview of diverse geochemical proxies frequently employed in the evaluation of provenance as well as to assess their capabilities and limitations in estimating the origin of sediment. Additionally, statistical methodologies might be advantageous for sediment source determination, although it is crucial to acknowledge their restrictions and to merge them with traditional geochemical and isotopic techniques. A multidisciplinary strategy that encompasses several techniques and methodologies is probable to yield the most sturdy and dependable outcomes for identifying sediment provenance.

Titanium transport and isotopic fractionation in the Critical Zone

Stable Ti isotopes have been applied in the detrital sediment record to reconstruct the bulk composition of Earth’s continental crust due to the relationship between magmatic differentiation and Ti isotopic compositions. However, no study has systematically evaluated the influence of provenance, physical, and chemical weathering on the composition of sediments relative to the protolith they originated from. To test the influence of these processes on Ti isotopic compositions we investigate the Ti isotope composition of 82 surface samples including loess, volcaniclastic rocks, river sediment, and two separate weathering profiles through igneous rocks, collected from a broad geographical area and a range of environmental conditions. Limited but significant Ti isotope fractionation exists in samples subjected to extreme chemical weathering processes, potentially as a result of elemental mobilization. For example, the δ49Ti isotopic composition of bauxites developed on Columbia River basalt varies by up to 0.1‰, becoming isotopically heavier with increasing weathering intensity. However, negligible variation in δ49Ti was found in a second profile of saprolites developed on weathered diabase. Titanium isotope variations in loess do not correlate with chemical weathering intensity or size sorting, but may instead be related to the provenance of the sediment. We find that the δ49Ti of Amazon River sediments is correlated with the Al/Zr ratio, indicating that δ49Ti is impacted by sediment sorting. At our study sites, the river averaged offset between the isotopic composition of the bedload and the suspended sediment fraction is 0.051‰, with the largest offset being + 0.116‰. Our data suggest that during chemical weathering, heavy Ti isotopes are preferentially incorporated into secondary minerals producing higher δ49Ti in intensely weathered soils. During fluvial transport, the Ti isotopic composition of fine-grained sediment is heavier than that of its coarser counterpart. Crustal protolith composition and sorting during transport and sedimentation have a stronger effect on the Ti isotopic composition than chemical weathering. Our results have implications for studies that utilize the Ti elemental concentration to calculate relative enrichment or depletion during chemical weathering and physical transport processes in the Critical Zone and for studies using Ti isotopes in terrigenous sediments to infer the composition of their provenance.

Grain size and mineralogical constraints on leaching in the bottom ashes from municipal solid waste incineration: a comparison of five plants in northern Italy

Introduction: Bottom ashes (BA) from municipal solid waste incinerators (MSWIs) are currently classified by the European Waste Catalogue as industrial non-hazardous waste. To promote their reuse, identification and characterization of the heavy metal-bearing phases (both glass and minerals), as well as their weathering behavior, must be addressed for what concern the chemical composition, mineralogical phases, and in high concentrations and pollutants’ mobility. An important point to be noted is whether the results from a given plant can be generalized.Materials and methods: In this work, BA from five waste-to-energy (WtE) plants in northern Italy were sorted based on different grain sizes. The area showed similar MSW production, collected from a culturally homogeneous area, and similar collection management. For each grain size, a mineralogical, chemical, and physical characterization was carried out using XRF, XRD, TGA, and the leaching test.Results and discussion: We found that for major elements, the average chemical composition of the incinerators is similar, with some differences in minor elements. Ferrara (FE) and Forlì-Cesena (FC) BA show portlandite, higher ettringite, and less amorphous than the Torino (TO), Parma (PR), and Piacenza (PC) BA. This affects the pH, the release, and toxicity of the leachates. In FE and FC ashes, ettringite is insoluble, and we not only have low sulfate but also Ni and Ba beyond reglementary limits, suggesting that Ni and Ba are present as hydroxides with portlandite, which are soluble. In TO, PR, and PC, Cr and sulfates are beyond limits, suggesting that Cr comes from dissolution in ettringite. Cu and Cl are always beyond limits; the dissolution of chlorides accounts for only 30%–35% of the global Cl leachate. We observe that in the assessment of the potential toxicity of the ashes, mineralogy has a higher effect than the bulk chemical composition. Grain size sorting, although useful together with other techniques, is not by itself able to comply with the PTE risk level.Conclusion: The findings contribute to the development of efficient treatment strategies for BA, highlighting the need for a more thorough investigation to understand composition and properties and to find innovative ways to reuse, promoting the circular economy and sustainable waste management practices.

Used vs. new: Does it have consequences for the performance of fishing gear?

Sea trials to test size selectivity equipment in trawls are often limited in time because they are costly, and the results can be influenced by multiple factors that are often overlooked. In the Barents Sea gadoid trawl fishery, the use of a size sorting grid in front of the codend is compulsory. The flexigrid, a netting section containing two flexible grids, is the most widely used grid section in this fishery. However, earlier selectivity studies with this device have shown inconclusive results. It has been speculated that the differences observed resulted from the difference in age and usage of the grid sections in the studies compared. To reveal whether potential changes in the device construction over time can lead to differences in size selection properties, we performed comparative fishing trials where we compared a brand new flexigrid section and a well-used flexigrid section used continuously by a commercial trawler for approximately four years. The results showed that the new flexigrid released significantly more cod below ∼60 cm than the used flexigrid. However, when the grids were fished with a subsequent diamond mesh codend, there was no difference in the overall selectivity of the two gears, meaning that the size selectivity in the codend compensates for the potential reduction in selectivity performance of the grids. This study shows the importance of considering the age and earlier use of size selection devices like sorting grids before they are compared with other devices, as their size selection properties can change significantly over time and with use.

A novel microparticle size sorting technology based on sheath flow stable expansion regimes

Previously we have originally reported and experimentally explored the stable expansion phenomenon of sheath flow in straight microchannels. The stable expansion phenomenon differs from the focused stable thread and diffusion, which are expected to appear based on current understanding. It happens within a specific range of Reynolds numbers in microchannels with specific aspect ratios. However, the mechanism insight and potential applications remained poorly understood. In this study, a comprehensive numerical model, involving laminar flow, diluted species transportation, and particle tracing, was established to provide a global transparent insight. Based on the simulation results, the root cause and mechanism of the stable expansion phenomenon were revealed as a dumbbell-shape deformation of the buffer–sample–buffer interface. In addition, a microparticle size sorting application based on these findings was proposed and conducted in both simulation and experiment. The results showed that 1 and 15 μm microparticles can be totally separated with nearly 100% purity. According to the advantages and potentials of this technique, it can be promising to approach a label-free microparticle sorting with a high throughput manner.

Impact of magmatic intrusion on diagenesis of shallow marine sandstones: An example from Qasim Formation, Northwest Saudi Arabia

Igneous intrusions are common in sedimentary basins, and their occurrence can significantly affect the diagenesis and reservoir quality evolution of sandstones, thereby strongly impacting their hydrocarbons-, geothermal-, and CO2-storage potentials. The Qasim sandstones in the Tabuk region (NW Saudi Arabia) experienced shallow burial diagenesis (<2 km) when the Tertiary magma intruded to form basaltic sills (0.4–4 m thick). The sedimentology, tectono-stratigraphic framework, provenance, and chemostratigraphy of the Qasim Formation have been extensively covered in the literature. However, the impact of the magmatic intrusion on diagenesis and reservoir quality evolution of the sandstones remains enigmatic. This study employed thin-section petrography, QEMSCAN, XRD, SEM, and energy-dispersive spectrometer analyses to investigate the role of magmatic intrusion on diagenesis and reservoir quality of the Qasim sandstones. The results of the study indicate that reservoir porosity is principally influenced by primary depositional characteristics (grain size and sorting), diagenetic alterations, and magmatic intrusions. Sandstones with coarser grain size and better sorting have the best intergranular porosity and vice versa. The “normal” diagenetic processes that have significantly affected the reservoir porosity of the sandstones occurred during both shallow burial (eodiagenesis) and uplift (telodiagenesis). The eogenetic alterations include mechanical compaction, early diagenetic cementation by calcite, pyrite, and kaolinite, whereas the telogenetic alterations include the formation of kaolinite, goethite, hematite. Overall, mechanical compaction is the main driver for porosity loss in the sandstones. The intrusion-related diagenetic processes include the dissolution of quartz grains, rounded quartz overgrowths, and calcite cement, and the transformation of kaolinite into dickite and chlorite. Detrital quartz and rounded quartz overgrowths have undergone dissolution due to acidic pore fluids from magma and high temperature. The transformation of kaolinite into dickite occurred in a dissolution-recrystallization fashion, and the amounts of kaolinite and dickite increase in fine-grained sediments away from sill contact due to hydrodynamic processes that deposited muscovite (which form kaolinite) in low energy environments. The chloritization of kaolinite was localized, and the magma-induced dissolution of goethite likely supplied the requisite high Fe content. Additionally, the intrusion has resulted in the dissolution of the early calcite and increase in porosity towards the sill contact. However, values for compactional porosity loss have relatively remained similar both at and away from the sill contact, as the sill is too thin to exert significant vertical loading. This study has relevance to understanding hydrocarbon exploration and exploitation in sediment-lava sequences, and to understanding the development of sediment-lava systems.

Geochemistry and zircon geochronology of the Late Triassic volcanic–sedimentary successions in northern Tibet: Implications for the provenance and tectonic evolution of the Mesozoic Qiangtang Basin

The Late Triassic volcanic–sedimentary successions deposited in the Qiangtang Basin are linked with the initiation of the Mesozoic Qiangtang Basin. However, the nature of this basin is hotly debated. Here, we synthesize petrographic, mineralogical, geochemical, and detrital zircon U–Pb age data of these volcanic–sedimentary successions to constrain their chemical weathering history, provenance, and tectonic setting and to reconstruct the tectonic evolution of the Mesozoic Qiangtang Basin. The chemical weathering indices, especially the chemical index of alteration (CIA), are controlled by grain size and hydraulic sorting rather than sediment recycling. Low compositional and textural maturities, high index of compositional variation (ICV) values, and low CIA and plagioclase index of alteration (PIA) values in most samples suggest that the sediments from the Fanghu and north Woruoshan sections are predominantly from low-maturity sources that may have experienced a weak chemical weathering history. Based on provenance-sensitive geochemical indices (La/Th versus Hf, La/Sc versus Co/Th, and Sc/Th versus Cr/Th) and petrographic features, the detrital contributions to the Nadi Kangri Formation siliciclastic rocks are predominantly proximal felsic rocks. Detrital zircon age spectra suggest that the Nadi Kangri Formation siliciclastic rocks exhibit a provenance change from the Fanghu to the well QK-1 area, although all of the rocks were derived from the Qiangtang terrane itself. The Fanghu section was primarily sourced from synchronous magmatic rocks and pre-Triassic strata from the North Qiangtang and Central uplift belt, while well QK-1 was primarily sourced from recycled sediments from the South Qiangtang and Central uplift belt. Therefore, we propose that the Nadi Kangri Formation volcanic–sedimentary successions were deposited in a rift basin and that its evolution was likely related to the interaction of the rapid expansion of the Meso-Tethys Oceanic crust, collapse of the collisional orogen, and rollback of the Jinsha Paleo-Tethys subducting slab during the Late Triassic.

Theory and practice of using cell strainers to sort Caenorhabditis elegans by size.

The nematode Caenorhabditis elegans is a model organism widely used in basic, translational, and industrial research. C. elegans development is characterized by five morphologically distinct stages, including four larval stages and the adult stage. Stages differ in a variety of aspects including size, gene expression, physiology, and behavior. Enrichment for a particular developmental stage is often the first step in experimental design. When many hundreds of worms are required, the standard methods of enrichment are to grow a synchronized population of hatchlings for a fixed time, or to sort a mixed population of worms according to size. Current size-sorting methods have higher throughput than synchronization and avoid its use of harsh chemicals. However, these size-sorting methods currently require expensive instrumentation or custom microfluidic devices, both of which are unavailable to the majority C. elegans laboratories. Accordingly, there is a need for inexpensive, accessible sorting strategies. We investigated the use of low-cost, commercially available cell strainers to filter C. elegans by size. We found that the probability of recovery after filtration as a function of body size for cell strainers of three different mesh sizes is well described by logistic functions. Application of these functions to predict filtration outcomes revealed non-ideal properties of filtration of worms by cell strainers that nevertheless enhanced filtration outcomes. Further, we found that serial filtration using a pair of strainers that have different mesh sizes can be used to enrich for particular larval stages with a purity close to that of synchronization, the most widely used enrichment method. Throughput of the cell strainer method, up to 14,000 worms per minute, greatly exceeds that of other enrichment methods. We conclude that size sorting by cell strainers is a useful addition to the array of existing methods for enrichment of particular developmental stages in C. elegans.

The utility of body size as a functional trait to link the past and present in a diverse reptile clade

Understanding the relationships between functional traits and environment is increasingly important for assessing ecosystem health and forecasting biotic responses to future environmental change. Taxon-free analyses of functional traits (ecometrics) allow for testing the performance of such traits through time, utilizing both the fossil record and paleoenvironmental proxies. Here, we test the role of body size as a functional trait with respect to climate, using turtles as a model system. We examine the influence of mass-specific metabolic rate as a functional factor in the sorting of body size with environmental temperature and investigate the utility of community body size composition as an ecometric correlated to climate variables. We then apply our results to the fossil record of the Plio-Pleistocene Shungura Formation in Ethiopia. Results show that turtle body sizes scale with mass-specific metabolic rate for larger taxa, but not for the majority of species, indicating that metabolism is not a primary driver of size. Body size ecometrics have stronger predictive power at continental than at global scales, but without a single, dominant predictive functional relationship. Application of ecometrics to the Shungura fossil record suggests that turtle paleocommunity ecometrics coarsely track independent paleoclimate estimates at local scales. We hypothesize that both human disruption and biotic interactions limit the ecometric fit of size to climate in this clade. Nonetheless, examination of the consistency of trait-environment relationships through deep and shallow time provides a means for testing anthropogenic influences on ecosystems.

Variable response of particles and inorganic carbon of two different soils during splash erosion

Splash erosion caused by raindrops is the first stage of water erosion that detaches and transports soil particles. As an important component of soil particles, soil inorganic carbon (SIC) contains little information on its redistribution during splash erosion. In this study, two soils, purple soil and loess, were selected and placed in a soil tray with a diameter of 20 cm at the center of a splash tray, and a rainfall simulator was used to simulate three raindrop energies in experiments to investigate the migration of soil particles and SIC. The results showed differences in the amount and selective migration of splashed material between the soils, which was evident in the obvious sorting of the particle size of purple soil with distance and the insignificant change of loess soil. The SIC sorting characteristic performed differently in the two soils, that is, the SIC content of purple soil increased with particle size, while that of loess was the opposite. The enrichment and depletion of SIC during splash erosion varied with soil type, and differed from the general enrichment of soil organic carbon (SOC). The SIC enrichment ratio (ERIC) of purple soil was generally > 1, whereas the ERIC values of loess were < 1. In addition, multiple linear regression (MLR) models were established to predict the SIC content of splashed material. Rainfall splash plays an important role in SIC migration and may further affect the carbon cycle. These preliminary results provide a basis for further study of SIC response to rainfall splash.

Dispersion of Bacteria by Low-Pressure Boiling: Life Detection in Enceladus' Plume Material.

The plume of Enceladus is thought to originate from the dispersion of a liquid source beneath the icy crust. Cryovolcanic activity on Enceladus may present a direct way of accessing material originating from the potentially habitable subsurface ocean. One way to test the hypothesis of whether life is present within the ocean of Enceladus would be to investigate the plume material for the presence of microbial life. In this study, we investigated the entrainment of Bacillus subtilis within Enceladus-like fluids under boiling conditions caused by exposure of the fluids to low pressure. We show that boiling, associated with exposure of a fluid to low pressure, works as a mechanism for dispersing bacteria in Enceladus plume-like environments. Exposure of Enceladus-type fluids (0.01-0.1 molal Na2CO3 and 0.05-0.2 molal NaCl) to low pressure (5 mbar) results in the dispersion of bacteria in droplets that evaporate to produce particles of salt. We find that, for particles with radius (r) ≤ 10 μm, the number of dispersed particles containing cells was between 7.7% and 10.9%. However, for larger particles 10 < r ≤ 50 μm, 64.4% and 56.4% contained cells for lower and upper end-member solutions, respectively. Our results suggest that the gravity-induced size sorting of plume particles will result in plume deposits closer to the vent source containing a larger volume of biological material than within the plume. If life is present in the ocean of Enceladus, we would expect that it would be effectively entrained and deposited on the surface; therefore, it would be accessible with a surface-lander-based instrument.

Particle size segregation and diffusion in saturated granular flows: Implications for grain sorting in debris flows

Sorting of rocks, boulders, and silt/sand-sized particles according to their size is a characteristic feature of debris flow deposits and is an active process during flow which significantly affects the mobility. The degree at which size sorting occurs in debris flows depends on the relative magnitudes of granular processes such as particle size segregation and diffusion. Since debris flows are fluid-saturated phenomena, accurate modelling of size sorting requires the understanding of the influence of fluids on these processes, which have not been systematically studied. Here, we present simulation results and the associated empirical expressions for particle size segregation and diffusion which take into account fluid effects due to buoyant and drag forces. These expressions are developed through scaling analysis of data obtained from coupled granular-fluid simulations of saturated bi-disperse mixtures under simple shear. We further show that using these scaling relationships, an existing segregation-diffusion continuum equation can be extended to model particle sorting in debris flows with various types of fluids.

Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics

Separation and clonal culture and growth kinetics analysis of target cells in a mixed population is critical for pathological research, disease diagnosis, and cell therapy. However, long-term culture with time-lapse imaging of the isolated cells for clonal analysis is still challenging. This paper reports a microfluidic device with four-level filtration channels and a pneumatic microvalve for size sorting and in situ clonal culture of single cells. The valve was on top of the filtration channels and used to direct fluid flow by membrane deformation during separation and long-term culture to avoid shear-induced cell deformation. Numerical simulations were performed to evaluate the influence of device parameters affecting the pressure drop across the filtration channels. Then, a droplet model was employed to evaluate the impact of cell viscosity, cell size, and channel width on the pressure drop inducing cell deformation. Experiments showed that filtration channels with a width of 7, 10, 13, or 17 μm successfully sorted K562 cells into four different size ranges at low driving pressure. The maximum efficiency of separating K562 cells from media and whole blood was 98.6% and 89.7%, respectively. Finally, the trapped single cells were cultured in situ for 4-7 days with time-lapse imaging to obtain the lineage trees and growth curves. Then, the time to the first division, variation of cell size before and after division, and cell fusion were investigated. This proved that cells at the G1 and G2 phases were of significantly distinct sizes. The microfluidic device for size sorting and clonal expansion will be of tremendous application potential in single-cell studies.