Smaller Aggregate Size Research Articles

Synthesis, Surface Activity, Emulsifiability and Bactericidal Performance of Zwitterionic Tetrameric Surfactants.

In this paper, a series of tetrameric surfactants (4CnSAZs, n = 12, 14, 16) endowed with zwitterionic characteristic were synthesized by a simple and convenient method and their structures were characterized by FT-IR, 1H NMR and elemental analysis. Their physicochemical properties were studied using the Wilhelmy plate method, fluorescence spectra and dynamic light scattering technique. 4CnSAZs have higher surface activities and tend to adsorb at the air/water surface rather than self-assembling in aqueous solution. The thermodynamic parameters obtained from surface tension measurements show that both processes of adsorption and micellization of 4CnSAZs are spontaneous and that the micellization processes of 4CnSAZs are entropy-driven processes. Both adsorption and micellization of 4CnSAZs are inclined to occur with the increase of alkyl chain length or temperature. For 4C12SAZs, there are only small-size aggregates (micelles), while the large aggregates (vesicles) are observed at the alkyl length of 4CnSAZs of 14 or 16. This shows that the alkyl chain length for oligomeric surfactants has a greater sensitivity for aggregate growth. The aggregate morphologies obtained from the calculated values of critical packing parameter (p) for 4C14SAZs and 4C16SAZs can be supported by the DLS measurement results. The test results obtained by the separation-water-time method show that 4CnSAZs have good emulsification performance and that the prepared emulsions appear to exit in the form of multiple emulsions. In addition, 4CnSAZs have good antibacterial activities against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The present study reveals the unique behavior of a zwitterionic tetrameric surfactant and may give new insights into molecular design and synthesis of a high degree of surfactants with different structure characteristics for potential application in various industrial fields.

Study of the binding interaction between modified glycinin and d-galactose and functions of the resulting complexes via multi-spectroscopy and molecular dynamics simulation

This study evaluated the effects of d-galactose (DG) concentration on the functional characteristics, physicochemical properties, structure and non-covalent binding ability of pH shifting treated soybean glycinin (S–11S). Multispectral and microscopic imaging analysis showed that the pH shifting induced soybean glycinin self-assembly into more stable small particle size aggregates, and the functional properties of S–11S were significantly enhanced. The introduction of DG further enhances the functional properties of S–11S, and the solubility and emulsification activity of the composite system are inversely proportional to the concentration of DG. In order to clarify the reason for the change, multispectral analysis found that the tertiary structure of S–11S was de-folded and the disordered structure was increased, which induced S–11S and DG to form smaller and stable nanoparticles than S–11S through hydrophobic forces and hydrogen bonding, and the optimal binding ratio was 10.8:1. In addition, the simulation results of molecular dynamics and molecular docking are in agreement with the experimental results. Therefore, the addition of DG can stabilize the overly discrete S–11S conformation and improve its functional characteristics. These results provide a theoretical basis for the application of modified protein and monosaccharide composite products in food and materials.

In vitro inhibition of α-Synuclein aggregation and disaggregation of preformed fibers by polyphenol hybrids with 2-conjugated benzothiazole

The misfolding and aggregation of α-Syn play a pivotal role in connecting diverse pathological pathways in Parkinson's disease (PD). Preserving α-Syn proteostasis and functionality by inhibiting its aggregation or disaggregating existing aggregates using suitable inhibitors represents a promising strategy for PD prevention and treatment. In this study, a series of benzothiazole-polyphenol hybrids was designed and synthesized. Three identified compounds exhibited notable inhibitory activities against α-Syn aggregation in vitro, with IC50 values in the low micromolar range. These inhibitors demonstrated sustained inhibitory effects throughout the entire aggregation process, stabilizing α-Syn proteostasis conformation. Moreover, the compounds effectively disintegrated preformed α-Syn oligomers and fibers, potentially by binding to specific domains within the fibers, inducing fibril instability, collapse, and ultimately resulting in smaller-sized aggregates and monomers. These findings offer valuable insights into the therapeutic potential of polyphenol hybrids with 2-conjugated benzothiazole targeting α-Syn aggregation in the treatment of PD.

Coinoculation of arbuscular mycorrhizal fungi and rhizobia stimulates atrazine dissipation by changing the atrazine-degrading bacterial community at the soil aggregate scale

As a potential low-cost and environmentally friendly strategy, bioremediation of herbicide polluted soil has attracted increasing attention. However, there is a lack of knowledge regarding the response of the atrazine-degrading bacterial community to coinoculation of arbuscular mycorrhizal (AM) fungi and rhizobia for atrazine dissipation. In this study, a pot experiment was conducted with AM fungi Glomus mosseae (AM), rhizobia Rhizobium trifolii TA-1 (R) and their coinoculation (AMR) with atrazine. In each treatment, the atrazine-degrading bacterial community of four soil size aggregates, namely large macroaggregates (LMa), small macroaggregates (SMa), microaggregates (Mia) and primary particles (P) were investigated. The results showed that the atrazine residue concentration was lowest in AMR, and that in LMa was also significantly lower than that in the other smaller aggregate sizes. Overall, inoculation, the aggregate fraction and their interaction had significant effects on soil TN, SOC, AP and pH. For the atrazine-degrading bacterial community, the Chao1 index increased with decreasing particle size, but the Shannon index decreased. Moreover, the abundances of the dominant atrazine-degrading bacterial genera Arthrobacter, Bacillus, Marmoricola and Nocardioides in the Mia and P particle size groups were greater than those in the LMa and SMa groups in each treatment. The bacterial communities in the Mia and P particle sizes in each treatment group were more complex. Therefore, coinoculation of AM fungi and rhizobia stimulated atrazine dissipation by changing the atrazine-degrading bacterial community, and the response of the atrazine-degrading bacterial community to each aggregate size varied depending on its distinct soil physicochemical properties.

Effect of land use conversion on the soil aggregate-associated microbial necromass carbon in estuarine wetland of the Pearl River in China

Microbial necromass carbon (MNC), as an important component of the soil organic carbon (SOC) pool in natural environments, has increasingly gained significant attention in recent years. However, the redistribution pattern of microbial necromass in aggregate fractions of estuarine soils induced by human activity still remain unclear. In this study, we assessed the effect of land use conversion on the redistribution and accumulation patterns of aggregate-associated MNC in estuarine wetland soils on the Pearl River estuary of China. Typical soil samples in 0–30 cm profiles under different land uses [Natural Wetland (NW), Farmland (FL), and Construction Land (CL)] were analyzed. The results showed that the NW conversion to FL and then CL contributed to the continuous losses of SOC, however, the aggregate-associated MNC content was higher in FL and lower in CL compared to NW. The MNC contribution to total SOC in aggregate fractions gradually increased from NW (16.41 % − 23.62 %) to CL (23.87 % − 30.98 %) and then FL (34.62 % − 38.83 %) in profiles. Fungal necromass C was the primary contributor to the total MNC in each aggregate fraction. In NW, the MNC was mainly present in large aggregate size, however, the MNC was gradually enriched into smaller size aggregates, especially for the bacterial necromass C, after the conversion from NW to FL or CL. Microbial biomass carbon had a direct and positive effect on the MNC in soil aggregates under different land uses, except for in silt + clay fraction from NW. Moreover, pH and soil moisture content were two key variables affecting the MNC content and accumulation by mediating the microbial biomass of different land uses, while the dominate factors controlling the MNC accumulation varied with size fractions in aggregates. Our results emphasize the influence of land use conversion on the soil aggregate-associated MNC accumulation in estuarine wetland.

Environmentally friendly concrete material with early strength and fast hardening using coal as an aggregate: a case for supporting empty roadways

In order to facilitate ventilation or transportation, many coal mines usually excavate an empty roadway in the middle of the mining working face. Under the influence of mining pressure, empty roadways are easily damaged, seriously threatening coal mines’ safety. Because the traditional support method of the empty roadway has the problems of low efficiency, high cost, and low strength, this paper researches the coal-based concrete support material with better effect. The grading test of coal aggregate was carried out, and it was found that the percentage of small-size aggregate greatly influenced the reduction of aggregate porosity in the stacked state. To solve the problems of the high cost of strengthening support methods such as high water material support in empty roadways, which could be more conducive to recovery and reduce coal quality. The research on empty roadways supports concrete material with coal as aggregate, making the working face recover coal resources safely and efficiently. The optimum gradation test of the total is to enhance the strength of support and solve the problem of low power and difficult cementation of coal. Based on the hydration analysis of cementitious material, the coal-based concrete, mainly composed of low-cost slag, is determined. The cementitious material with simple composition, rapid setting, and fast hardening was found. It is proved that the mechanical properties and molding effect of coal-based concrete support composed of gypsum, GGBFS, and clinkercement are better than those of commercial cement. The structural model of “surrounding rock-empty roadways-coal pillar-support” is established. The calculation method and check basis of empty roadways support resistance N1 are obtained. The results of industrial application verified that coal-based concrete could effectively protect empty roadways without affecting the productivity of coal mines.

Durability of the noise-reduction performance of the PMSMA and CRSMA pavements - a case study in Hong Kong

Tyre/road interaction noise is one of the main sources of urban traffic noise, which has aroused public concern about health and living environment. Low-noise road surface (LNRS) is a feasible solution to reduce tyre/road noise from its source. As a high-density city, Hong Kong has studied the noise-reduction performance of different LNRS materials in the past decade, such as the crumbed rubber modified stone mastic asphalt (CRSMA) and polymer modified stone mastic asphalt (PMSMA) with a small maximum aggregate size. This study aims to evaluate and compare the durabilities of the noise-reduction performance of CRSMA and PMSMA pavements. The close-proximity (CPX) method was applied to collect the tyre/road noise data over three consecutive years after the pavements were constructed. The sound pressure levels and spectrograms were investigated to compare the noise reduction durabilities of the two LNRS materials. The results showed that the CRSMA6 pavement showed good and relatively stable noise reduction performance during the whole observation period. In contrast, although PMSMA6 presented a lower noise level than CRSMA6 at the early stage, its noise reduction property gradually deteriorated. PMSMA6's noise value surpassed CRSMA6 in the third year.

Suppression of human lysozyme aggregation by a novel copper-based complex of 3,4-dimethoxycinnamic acid

In this work, a new Cu(II)-based complex as a chemotherapeutic drug agent, formulated as[Cu(DCA)4(H2O)2]⋅4H2O⋅4MeOH, (DCA = 3,4-dimethoxycinnamic acid), namely 1 was successfully synthesized utilizing DCA as a ligand to arrest fibrillation in Human lysozyme. The 1 was thoroughly characterized by single crystal X-ray diffraction (SC-XRD), spectroscopic (UV-Vis and FTIR) techniques, PXRD, and TGA analysis. Its crystal structure reveals a paddle wheel network around central copper metal ions. The Cu(II) metal ions exhibit a distorted square pyramidal configuration. The fluorescence titration studies showed moderate binding interaction of 1 with HuL with Ka of 6.3x105 M−1 at pH-2, 25 °C due to its interaction withAsp53, Tyr63, Val110, and Ala111 as shown by docking and simulation studies. 1suppresses the HuL fibrillation in a concentration-dependent manner, as demonstrated by ThT assay. At 200 µM concentration, it leads to the formation of smaller species of the protein in comparison to the control sample, as suggested by Light Scattering studies. The species formed are less hydrophobic and retain their native α-helix structure compared to the control samples, which are hydrophobic and form β-sheet rich amyloids as shown by ANS hydrophobicity assay and CD spectroscopy, respectively. Furthermore, morphological analysis of the species by AFM has demonstrated that, unlike mature amyloid fibrils in the control sample, HuL forms small-size aggregates in the presence of 1 under similar fibrillation conditions. It can be concluded that 1 effectively suppresses HuL fibrillation due to moderate binding to the protein. Communicated by Ramaswamy H. Sarma

Catalyst Aggregate Size Effect on the Mass Transport Properties of Non-Noble Metal Catalyst Layers for PEMFC Cathodes

Non-noble metal catalysts (NNMCs) are regarded as a promising alternative to the costly Pt-based materials required to catalyze the oxygen reduction reaction (ORR) in proton exchange membrane fuel cell (PEMFC) cathodes. However, the large diversity of NNMC synthesis approaches reported in the literature results in materials featuring a wide variety of particle sizes and morphologies, and the effect of these properties on these catalysts’ PEMFC performance remains poorly understood. To shed light on this matter, in this work we studied the physical and electrochemical properties of NNMC layers prepared from materials featuring broadly different aggregate sizes, whereby this property was tuned by ball milling the precursors used in the NNMCs’ synthesis in the absence vs presence of a solvent. This led to two NNMCs featuring similar Fe-speciations and ORR-activities, but with vastly different aggregate sizes of >5 μm vs ≈100 nm, respectively. Following the extensive characterization of catalyst layers (CLs) prepared with these materials via electron microscopy and X-ray tomography, PEMFC tests at different loadings unveiled that the smaller aggregate size and ≈20% higher porosity of the CL prepared from the wet-milled sample resulted in an improvement of its mass transport properties (as well as a ≈2-fold enhancement of its peak power density under H2/air operation) over the dry-milled material.

Effect of aggregate size on water distribution and pore fractal characteristics during hydration of cement mortar based on low-field NMR technology

The influence of aggregate size (AS) on the hydration of cement mortar is very important for improving the quality and stability of cement mortar. This study aims to study the effect of AS on water distribution and pore structure characteristics on the early-age hydration of cement mortar by low-field NMR technology. The hydration degree and fractal dimension of the hydration process were calculated by the T2 spectrum. The water distribution models at different times and locations during the hydration process were established based on the MRI bitmap data. The variation law of water volume at different locations was studied. The results indicate that: In the process of hydration, some of the water is consumed by the hydration reaction, and some of the water oozes out. The water in the large pores flows to the small pores under the action of hydrostatic pressure. AS affected the uniformity of the cement mortar at the initial state, and the gradation of the sand could improve the uniformity. The smaller AS was, the more complex the pore water distribution and changed during hydration. The bleeding mass was directly proportional to the porosity and inversely proportional to the specific surface area of particles. The effect of porosity on bleeding mass was more significant than AS. Pore fractal dimension was negatively correlated with aggregate diameter. In the initial stage, when the aggregate composition became complex, the change of fractal dimension with time also became complex. The fractal dimension increased gradually with the increase of hydration time. The gel pores (G) did not have fractal characteristics from beginning to end, capillary pores (C) and transition pores (T) had fractal characteristics after a particular time of hydration reaction. Although the air-voids (A) had fractal characteristics from beginning to end, they were not continuous.

Effect of maximum coarse aggregate size upon shear strengthening of RC beams using NSM-CFRP strips

Reinforced concrete (RC) is widely used because of its many desirable characteristics, such as high strength, fire resistance, and durability. However, structures that are made of concrete may need strengthening or repairing due to concrete deterioration, the harshness of the environment, steel corrosion, errors in design, and damage caused by earthquake load. The use of composite materials has recently become more popular for strengthening and repairing damaged concrete structures. It is also known that properties of the aggregates, such as shape, size, and surface texture, have important influences on the behavior of concrete. In fact, many studies have shown that changes in coarse aggregate will affect the concrete's strength and fracture properties. Towards this end, very little research has been carried out on the effect of maximum aggregate size (MAS) on the shear behavior of reinforced concrete beams. The impact of maximum coarse aggregate size on shear strengthening using the methods proposed in this study was not studied. This research is driven by both environmental and economic aspects where the use of large-size coarse aggregates provides a large volume and less void space compared to small-size aggregates. From an environmental perspective, such an approach will reduce the need for cement in concrete unit volume, hence reducing the pollution. The main purpose of this study is to examine the behavior of reinforced concrete beams under general loading and how this is affected by using different coarse aggregate sizes. This work’s main objective is to investigate the effect of maximum coarse aggregate size on the shear behavior of reinforced concrete beams and study the effectiveness of using the Near Surface Mounted (NSM) – Carbon Fiber Reinforced Polymer (CFRP) strips technique on the shear strengthening of the RC beams. The experimental variables of the study consist of different maximum coarse aggregate sizes (9.5 mm, 12.5 mm, 19 mm, and 25 mm), Carbon Fiber Reinforced Polymer (CFRP) strips spacing (75 mm and 150 mm), and concrete compressive strength (25 MPa and 40 MPa). Fourteen RC beams (150 mm × 200 mm × 1150 mm) were cast using six mixes of concrete to be prepared and tested. The experimental results showed that using maximum coarse aggregate size has a positive effect on ultimate shear strength, maximum deflections, toughness, and stiffness of strengthened RC beams. Using NSM-CFRP strips change the failure mode from pure shear for the control beams to shear-flexural failure for the strengthened beams, which is an ideal enhancement.

Experimental exploration of digitally fabricated connections for structural concrete

Connections are a persistent challenge for traditional reinforced concrete construction and even more for digital fabrication with concrete (DFC). The latter, on the other hand, opens up new possibilities for producing connections with tailored geometries and surface roughness. Based on the findings of an exploratory, experimental campaign, this paper discusses the design, fabrication and structural testing of digitally fabricated joints using the DFC technology Eggshell to 3D print the formwork for joint surfaces in an additive manufacturing process. The programme includes (i) unreinforced and reinforced construction joints whose joint surface texture was varied to control the roughness and (ii) digitally fabricated dry keyed joints with varying geometry. Both, the construction joints as well as the dry keyed joints, were produced using set-on-demand concrete with a relatively small maximum aggregate size of 0 to 4 mm – i.e., strictly speaking, mortar rather than concrete – as typically applied in DFC. The experimental campaign included thorough instrumentation: a 3D scan of the joint surface before testing and high-resolution digital image correlation (DIC) combined with distributed fibre optical sensing (DFOS) during testing. The deviations from the targeted surface geometry, determined with 3D scans, were below 2 mm, with a standard deviation between 0.4 mm and 0.7 mm for keyed joints. The high-resolution DIC allowed measuring the compressive strains locally at (i) the corrugation of unreinforced or reinforced construction joints and (ii) the key chamfer of dry joints and, hence, studying strain variations over the height of the joints and the efficiency of different keys with varying dimensions. Finally, the shear transfer capacity, evaluated in a push-off test setup, showed that all digitally fabricated joints met or even outperformed the requirements defined in pertinent design codes despite the smaller aggregate size.

Aggregate-Exposing Operation Parameters, Laboratory and Road Performances of Exposed-Aggregate Concrete Pavement Applied in Long Tunnel

Abstract In this paper, exposed-aggregate concrete (EAC) samples, including EAC1 samples with a small aggregate size and EAC2 samples with a large aggregate size, were prepared. The effects of ambient temperature on the waiting time for the spraying of the aggregate-exposing agent (AEG) and the waiting time for the brushing of surface mortar were studied. Furthermore, the optimate spraying dosage of the AEG was determined, and a comparison was undertaken between the antiwearing properties of EAC1, EAC2, and grooved concrete samples. The correlation between the proposed aggregate-exposing depth (AED) indicator and the mean texture depth (MTD) indicator was examined in this study. Stone Mastic Asphalt-13 (SMA-13), EAC1, EAC2, and grooved concrete pavements were paved in a long tunnel, and their macrotexture, skid resistance, noise reduction, and antiglaring performance were compared. As per the results, a good, linear correlation was established between the AED and MTD indicators. With the increase in ambient temperature, the disappearance time of water film on the surface of cement concrete continues to shorten. The earliest, optimum, and latest waiting times for the brushing of EAC are significantly advanced, and the brushing window period continues to shorten. The recommended spraying dosage of 6 % AEG solution is 250–300 g/m2. Under the same spraying dosage of AEG solution, increasing the aggregate size improves the macrotexture of the EAC surface. Note that the skid resistance performance of EAC is not necessarily proportional to its macrotexture. Significantly large aggregate size is disadvantageous to the skid resistance performance of the pavement. The anti-attenuation performance of macrotexture, skid resistance performance, noise reduction performance, and antiglaring performance of EAC pavements are similar to that of the Stone Mastic Asphalt-13 (SMA-13) pavement and significantly better than those of grooved pavement. Moreover, the aforementioned performances of EAC1 pavement with small particle size are found to be better.

Mechanical Properties of Pervious Recycled Aggregate Concrete Reinforced with Sackcloth Fibers (SF)

The excessive production of construction waste is a significant concern as it requires proper disposal and may become economically unfeasible. Reusing construction waste in producing new concrete can substantially reduce the disposal requirements of construction waste. In addition, this results in a sustainable solution for the rapidly depleting natural resources of concrete. Pervious concrete may contain up to 80% coarse aggregates and could be an exceptional host for reusing construction waste. This study aimed to investigate the mechanical properties of pervious concrete constructed with natural and recycled aggregates. The substandard properties of recycled aggregates were improved by adding natural fibers from sackcloth. This study presents an experimental program on 45 samples of pervious concrete with air void ratios and the size of coarse aggregates as the parameters of interest. The compressive strength of the pervious concrete decreased by increasing the air void ratio regardless of the size of the aggregates. The type of aggregates did not influence the permeability of pervious concrete, and the maximum temperature in pervious concrete increased as the quantity of air void ratios increased. The decrease in compressive strength was 40–60% as the void ratio was increased from 10–30% for all types of concrete mixes, such as natural and recycled aggregates. The permeability of small-size aggregates with 10% designed air void ratios for natural and recycled aggregates with sackcloth was 0.705 cm/s.

Influence of aggregate structure on the compressibility of an unsaturated compacted silty sand

It is well recognised that the compressibility of unsaturated soils is affected by soil structure. However, the effects of soil aggregation and pore structure on the compressibility behaviour of unsaturated soil are still not well understood. In this study, the effects of soil aggregation and pore structure on the compressibility of unsaturated silty sand were investigated. Specimens were purposely prepared at two compaction water contents to similar void ratios. They were saturated after compaction and dried to suction values of 0, 50, 150, or 300 kPa following a similar hydraulic loading path, after which they were subjected to isotropic loading and unloading. Mercury intrusion porosimetry (MIP) and micro-X-ray computed tomography (μ-XCT) were used for microstructural studies. At a given suction, specimens compacted on the dry side are less aggregated (smaller aggregate sizes) showing higher compressibility, whiles specimens compacted on the wet side are highly aggregated (larger aggregate sizes) revealing lower compressibility. The low compressibility of the highly aggregated soil is attributed to the additional drying-induced intra-aggregate strength due to the suction stiffening effects from meniscus water within the aggregates.

Bioconjugated β-Cyclodextrin-Perfluorohexane Nanocone Clusters as Functional Nanoparticles for Nanoparticle-Mediated Histotripsy.

Nanocone clusters (NCCs) are new-generation agents of nanoparticle-mediated histotripsy (NMH) recently developed to address the limitations of previously designed nanodroplets (NDs). NCCs can be obtained by simply mixing FDA-approved cyclodextrins (CD) and suitable perfluorocarbons (PFCs), which result in smaller size aggregates, detectable PFC amount, and more stable long-term storage since the obtained powder can be stored and redispersed as needed. Previous experimental and computational studies showed that NCCs consist of an organization of inclusion complexes of CD and PFC around free PFC droplets, and their aggregate behavior depends on the localization of PFC in the cavity and the water solubility of CD derivatives. It has been shown that β-cyclodextrin (βCD) and perfluorohexane (PFH) are ideal candidates for NCCs that can be isolated as a powder with high PFC content among various CD and PFC derivatives. This study focuses on the further development of the selected NCC composition to enhance the potential of NMH therapy while also enabling more detailed future experiments in vitro and in vivo. It is aimed to show the bioconjugation potential of NCCs through the example of the most commonly used functionalization methods such as targeting, PEGylation, and fluorescent labeling. For this purpose, βCD as a building block was monofunctionalized with groups such as azide, alkyne, and amine groups that allow for effective coupling reactions such as the "click" reaction and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) coupling. These monofunctional βCDs were used as building blocks of NCCs in the presence of PFH to obtain functional NCCs as precursors of bioconjugation. EPPT1 as a synthetic peptide specific to uMUC1 and folic acid (FA) as the most commonly used targeting agent along with PEGylation were successfully shown as bioconjugation examples. Lastly, fluorescently labeled NCCs were obtained via fluorescein isothiocyanate (FITC) and alkyne functional NCC reaction through propargyl amine and isothiocyanate group reaction. The obtained bioconjugates were tested in vitro to validate the conjugation, and the ability to lower the histotripsy cavitation threshold, which is necessary for NMH, was demonstrated for all bioconjugates. Overall, the results showed that all obtained bioconjugates successfully lowered the cavitation threshold pressure while also fulfilling the desired bioconjugation metrics to serve as improved tools to enhance NMH as a targeted noninvasive ablation method.

Investigations on asphaltene aggregate formation by high-field diffusion NMR and low-field ghost solvent NMR relaxometry

Aggregation of asphaltenes remains a scientific challenge. In the present contribution, at first, transverse magnetization relaxation (T2) data from low-field NMR measurements of the asphaltenes from heavy, medium-heavy, or light crude oil samples were recovered. Following this, diffusion ordered spectroscopy nuclear magnetic resonance (NMR) spectra of these asphaltenes were acquired to obtain average molecular weights (M w) of asphaltene aggregates. Analyzing the relaxation and diffusion behavior of asphaltene aggregates as a function of solution percentage and hence molecular weights are utilized to explain the difference between light, medium-heavy and heavy crude oil asphaltenes in aggregate formation. The main results suggest that light crude oil asphaltenes form a small but significant number of aggregates. In contrast, heavy crude oil asphaltenes demonstrate a more complicated picture at high concentrations with small, medium, and large aggregates. The relaxation behavior of medium-heavy asphaltenes is similar to those of heavy crude oil asphaltenes in diluted solutions and similar to light crude oil asphaltenes in concentrated solutions. Diffusion NMR results showed that the light crude oil asphaltene samples have a relatively smaller aggregate size, higher diffusion coefficient, and hence a smaller molecular weight than the samples extracted from heavy crude oils.

Quantitative super-resolution imaging of pathological aggregates reveals distinct toxicity profiles in different synucleinopathies

Protein aggregation is a hallmark of major neurodegenerative disorders. Increasing data suggest that smaller aggregates cause higher toxic response than filamentous aggregates (fibrils). However, the size of small aggregates has challenged their detection within biologically relevant environments. Here, we report approaches to quantitatively super-resolve aggregates in live cells and ex vivo brain tissues. We show that Amytracker 630 (AT630), a commercial aggregate-activated fluorophore, has outstanding photophysical properties that enable super-resolution imaging of α-synuclein, tau, and amyloid-β aggregates, achieving ∼4 nm precision. Applying AT630 to AppNL-G-F mouse brain tissues or aggregates extracted from a Parkinson's disease donor, we demonstrate excellent agreement with antibodies specific for amyloid-β or α-synuclein, respectively, confirming the specificity of AT630. Subsequently, we use AT630 to reveal a linear relationship between α-synuclein aggregate size and cellular toxicity and discovered that aggregates smaller than 450 ± 60 nm (aggregate450nm) readily penetrated the plasma membrane. We determine aggregate450nm concentrations in six Parkinson's disease and dementia with Lewy bodies donor samples and show that aggregates in different synucleinopathies demonstrate distinct potency in toxicity. We further show that cell-penetrating aggregates are surrounded by proteasomes, which assemble into foci to gradually process aggregates. Our results suggest that the plasma membrane effectively filters out fibrils but is vulnerable to penetration by aggregates of 450 ± 60 nm. Together, our findings present an exciting strategy to determine specificity of aggregate toxicity within heterogeneous samples. Our approach to quantitatively measure these toxic aggregates in biological environments opens possibilities to molecular examinations of disease mechanisms under physiological conditions.

Study on anisotropy of 3D printing PVA fiber reinforced concrete using destructive and non-destructive testing methods

To further understand the mechanical properties and anisotropy characteristics of 3D printing PVA fiber reinforced concrete (PFRC). This study first obtained 3D printing PFRC mixes with superior mechanical properties through slump tests, penetration resistance tests, printability tests, and flexural tests. Second, the fiber reinforcement mechanisms and anisotropy characteristics of PFRC were analyzed by nondestructive ultrasonic pulse velocity tests, destructive mechanical property tests, and scanning electron microscope tests. Third, the correlation between mechanical properties and ultrasonic signals was analyzed, and the conversion criteria were further established. Moreover, the application range of conversion criteria was also determined. The test results showed that the optimum dosage of PVA fibers is 0.5 wt% for 3D printing FRPC, and the highest cube compressive strength and flexural strength are 98.2 MPa and 6.8 MPa, respectively. The mechanical properties and acoustics of 3D printed specimens showed significant anisotropy. The conversion criteria show a good agreement with the test data and are applicable to 3D printing fiber concrete with a small aggregate size.

Aggregation‐dependent phosphorus adsorption under different land uses of district Kupwara of Kashmir Valley

AbstractBackgroundPhosphorus (P) is among the essential elements for plant growth and one of the main elements of fertilizers. Decreased availability of P may limit agricultural production in the coming years. The magnitude of soil aggregation influences phosphorus access to mineral surfaces.AimThe present study aims to determine phosphorus adsorption processes affected by soil aggregation under different land‐use systems.MethodsThe distribution of soil aggregates was determined in representative soil samples in the district Kupwara of Kashmir Valley in India. To predict the phosphorus fertilizer requirement of a particular soil, we used the Freundlich adsorption equation and Langmuir equation and drew a clear comparison between these two models.Results and discussionMaximum phosphorus (P) adsorption was recorded at the smallest aggregate size, 0.5–0.1 mm. However, soil aggregates >2.0 mm (the largest category) adsorbed the least amount of P. Our results revealed that increasing the addition of P to the soil decreased the percentage of adsorbed P regardless of aggregate size. The maximum P adsorption of different size aggregates varied between 1869–1924, 1872–1900, 1718–1739, and 1800–1890 mg P kg–1 in irrigated agriculture, forest, orchard and rainfed agriculture soils, respectively. The variation in P adsorption parameters across the different land uses was attributed to their mean weight diameter difference. The maximum bonding energy in the forest resulted in higher P adsorption. Langmuir and Freundlich's adsorption equations were fitted to each soil aggregate size and land‐use system.ConclusionOur results revealed that for all soil aggregate sizes and land use systems, the Freundlich adsorption equation performs better than the Langmuir equation.