Evidence for single-use Negative Pressure Wound Therapy (sNPWT) in managing surgical incisions is demonstrated across surgical indications. However, there is limited evidence of surgical wounds using large real world database. The aim of this study was to examine wound closure in sNPWT and tNPWT in surgical wounds patients from a real-world evidence perspective This retrospective cohort study was conducted by identifying surgical wounds from an outpatient wound clinic EMR from 2014 to 2018. The treatment period began with the first application of NPWT and continued until the LEU closed or the patient was lost to follow-up. Patients were excluded if they received both types of NPWT or had missing wound clinical information. Patients were matched on demographic characteristics, comorbidities and baseline wound surface area, wound depth, wound age using propensity score matching. Wound closure was reported descriptively and estimated using multiple logistic regression analysis. Number of wound closed and surface area was reported at monthly interval. One-to-one propensity score matching method matched 490 sNPWT to tNPWT patients with baseline surface area of 8.10 cm2 and 8.56 cm2, respectively. Descriptive analysis estimated that sNPWT improved wound closure (43.47% vs 39.39%; p-value=0.1946) while regression analysis estimated sNPWT wounds were 37% more likely to close (OR=1.37; 95% CI=1.01-1.87; p-value=0.0445). In addition, sNPWT patients had higher number of wound closed and higher surface area reduction at monthly follow-up period. Before matching, there were 568 sNPWT and 1,787 tNPWT patients with mean initial surface area of 7.36 cm2 and 17.76 cm2, respectively and descriptive analysis estimated that sNPWT had statistically significant higher wound closure rate (44.37% vs 37.88%; p-value=0.0059). Single-use Negative pressure wound therapy is associated with significantly higher wound closure rate compared to traditional negative pressure wound therapy in surgical wounds.