Similar Depth Research Articles

Depth use of wild Atlantic salmon post-smolts migrating through fjords

Juvenile Atlantic salmon (Salmo salar), known as post-smolt as they enter the sea, undergo an arduous migration from their natal rivers to their feeding grounds in the North Atlantic Ocean. It has become increasingly important to understand how post-smolts use the fjord environment as they migrate in order to properly assess the potential threats posed by large-scale salmon farming. Chief among these threats is the increased numbers of ectoparasitic salmon lice (Lepeophtheirus salmonis) inhabiting the water column, which are known to have specific depth preferences based on light, temperature, and salinity. Here, we present data on the depth use of wild Atlantic salmon post-smolts migrating through fjords. We aimed to investigate to what extent depth use varied throughout the fjord and from year to year. Using acoustic telemetry, tagged fish from four rivers in two fjords in western Norway were monitored as they migrated towards the open sea during two consecutive years. We found that post-smolts predominantly migrated in the top three meters of the water column throughout the length of both fjord systems. Among 61 successful migrants and 7013 detections, 98% of detections were in the top three meters of the water column. This corresponds well with past findings showing similar depth use in hatchery-reared smolt and in adult Atlantic salmon kelts returning to their feeding grounds after spawning. We found little evidence of a consistent diel pattern in depth use. Our results support assumptions of representative sampling when trawling the upper portion of the water column for post-smolts in order to estimate sea lice infection rates and may improve the precision of efforts to model salmon lice infection risk. The results may also be valuable in evaluating other threats to wild salmon.

Rapid Down‐Slope Transport of Fresh Dissolved Organic Matter to the Deep Ocean in the Eastern North Atlantic

AbstractIntense convective mixing in the central North Atlantic is a major gateway for dissolved organic matter (DOM) into the deep ocean, sustaining elevated dissolved organic carbon (DOC) concentrations. Rapid down‐slope transport on adjacent Irish and Hebrides Margins represents another, less‐explored mechanism contributing to the deep‐sea DOM reservoir. Our analyses of solid‐phase extractable DOM (SPE‐DOM) in bottom waters in this region showed 7–11 μM higher DOC concentration and 190–330 years youngerSPE‐DOM radiocarbon ages compared to similar depths in the open eastern North Atlantic. We estimated a down‐slope DOC flux of 43 Tg C yr−1 from the Irish and Hebrides shelves. During transport, conservative mixing, dominated by physical rather than biological/chemical processes, determined the molecular DOM composition, while minor particulate organic matter degradation introduced less‐refractory DOM with terrigenous characteristics. Thus, rapid down‐slope transport emerges as an efficient conduit for delivering fresh DOM into the deep ocean.

Numerical and analytical study on the punching shear capacity prediction for eccentrically loaded flat slabs with openings

The punching shear behavior of interior flat slab was examined in this study in the absence of shear reinforcement using numerical and analytical techniques. The effect of sensitive parameters was investigated and their influence was examined and reported including the opening existence and location, loading eccentricity, and slab height. The effect of the reinforcement ratio is neglected in most of the literature models and code provisions with the influence of loading eccentricity being addressed inappropriately. Therefore, this study adopted the nonlinear finite element analysis (NLFEA) method for the numerical part where the flat slab punching shear behavior was studied in detail. Results were compared in their behavior at both cracking and ultimate stages, absorbed energy, elastic and plastic stiffnesses, and general trend lines were provided. The effect of the reinforcement ratio was assessed indirectly with slabs of similar reinforcement area and different slab effective depths (65, 95, 125, and 155) mm where a 25 mm concrete cover was used. In addition, the NLFEA punching shear capacity results were compared with three international codes (American Concrete Institute (ACI318-119), EuroCode (EC2), and Model Code (MC2010)) to highlight their potential predictability and weakness points. It was found that the MC2010 has the most conservative prediction, followed by the EC2 and the ACI318-19 predictions. Therefore, two modification parameters were proposed for the ACI318-19 punching shear capacity calculation with R2 value of 0.98. The modified version of the ACI318-19 was validated using experimental data from the literature proving a high prediction capability.

Adapting generalized suitability curves from Brown trout to Minnow using 1D and 2D aquatic habitat models

The article focuses on adapting generalized depth preference and flow velocity characteristics from Brown trout (Salmo trutta morpha fario) to the Minnow (Phoxinus phoxinus). The results obtained were used to model habitat suitability with 1D and 2D models. Since 1995, research on assessing aquatic habitat quality has been ongoing on 77 mountain streams in Slovakia. This assessment employs a System of Environmental Flow Analysis (SEFA), which is based on the Instream Flow Incremental Methodology (IFIM). In most sections, Brown trout occurred in representative numbers. Brown trout habitat preferences were derived and generalized, and represented by suitability curves. Minnows were present in sufficient numbers for the derivation of suitability curves only on some streams, making direct generalization from the measured data unrepresentative. The measurement results showed that Minnow has similar depth and flow rate preferences to Brown trout. Therefore, it can be assumed that it is possible to adapt the generalised suitability curves from Brown trout to Minnow. This expansion enabled us to broaden the assessment of habitat quality using the SEFA model to mountain streams that are dominated by Minnows and where there is insufficient Brown trout presence. Verification of parameter adaptation from Brown trout to Minnow was conducted in 11 sections of mountain streams. We discuss the performance of habitat quality modeling, concerning the fundamental hydraulic characteristics of streams, using both 1D and 2D models. The results of the 2D modeling are presented for a sub-mountain stream.

Confusion of extragalactic sources in the far-infrared: A baseline assessment of the performance of PRIMAger in intensity and polarization

Because of their limited angular resolution, far-infrared telescopes are usually affected by the confusion phenomenon. Since several galaxies can be located in the same instrumental beam, only the brightest objects emerge from the fluctuations caused by fainter sources. The PRobe far-Infrared Mission for Astrophysics imager (PRIMAger) will observe the mid- and far-infrared (25--235\,mu m) sky both in intensity and polarization. We aim to provide predictions of the confusion level and its consequences for future surveys. We produced simulated PRIMAger maps affected only by the confusion noise using the simulated infrared extragalactic sky (SIDES) semi-empirical simulation. We then estimated the confusion limit in these maps and extracted the sources using a basic blind extractor. By comparing the input galaxy catalog and the extracted source catalog, we derived various performance metrics as completeness, purity, and the accuracy of various measurements (e.g., the flux density in intensity and polarization or the polarization angle). In intensity maps, we predict that the confusion limit increases rapidly with increasing wavelength (from 21\,mu Jy at 25\,mu m to 46\,mJy at 235\,mu m). The confusion limit in polarization maps is more than two orders of magnitude lower (from 0.03\,mJy at 96\,mu m to 0.25\,mJy at 235\,mu m). Both in intensity and polarization maps, the measured (polarized) flux density is dominated by the brightest galaxy in the beam, but other objects also contribute in intensity maps at longer wavelengths (sim 30\,<!PCT!> at 235\,mu m). We also show that galaxy clustering has a mild impact on confusion in intensity maps (up to 25\,<!PCT!>), while it is negligible in polarization maps. In intensity maps, a basic blind extraction will be sufficient to detect galaxies at the knee of the luminosity function up to zsim 3 and 1011\,M$_ main-sequence galaxies up to zsim 5. In polarization for the most conservative sensitivity forecast (payload requirements), sim 200 galaxies can be detected up to z=1.5 in two 1\,500\,h surveys covering 1\,deg$^2$ and 10\,deg$^2$. For a conservative sensitivity estimate, we expect sim 8\,000 detections up to z=2.5, opening a totally new window on the high-z dust polarization. Finally, we show that intensity surveys at short wavelengths and polarization surveys at long wavelengths tend to reach confusion at similar depth. There is thus a strong synergy between them.

Dentinal Tubule Occlusion and Dentin Permeability Efficacy of Silver Diamine Fluoride Solutions.

This invitro study compared the effect of two commercially available silver diamine fluoride (SDF) products on dentinal tubule (DT) occlusion and dentin permeability. Sixty-six dentin discs (1.0 mm thick) were divided equally into six groups: acid-etched with no treatment (negative control-NC), etched and sealed with nail varnish (positive control-PC), non-etched and treated with Advantage Arrest (AA), non-etched and treated with Topamine (TP), etched and treated with AA, and etched and treated with TP. Eight specimens from each group were tested for permeability using the dye percolation method. The remaining three samples from each group were assessed for dentinal tubule occlusion, depth of SDF penetration, and elemental analysis using a scanning electron microscope coupled with an energy-dispersive X-ray (SEM/EDX) system. One-way ANOVA and Tukey's post hoc test were used to analyze the results. TP exhibited lower (p < 0.05) dye percolation compared to AA. While both SDF materials showed a statistically similar (p > 0.05) penetration depth in the DT, none of them had the ability to completely occlude all the DTs. AA exhibited more silver and fluoride ion deposition on etched dentin than TP, and both materials showed a similar increase in the deposition of Ca and P compared to NC. Whereas the effectiveness of dentin tubule (DT) occlusion varies based on the material used, both tested SDF products exhibit the capability to seal dentinal tubules, decrease dentin permeability, and promote dentin remineralization. These findings suggest their potential advantage in managing dentin hypersensitivity.

Variable-energy cocktail beam technology for investigating synergistic damage in nuclear materials on LEAF platform

This study addresses the critical issue of synergistic radiation damage in structural materials of fusion reactors, focusing on the interaction between the displacement defects and transmutation-produced hydrogen and helium. These effects are simulated and investigated by employing the advanced multi-beam ion implantation capabilities of the LEAF (Low Energy high intensity highly charged ion Accelerator Facility) platform. Firstly, high-intensity cocktail beams, such as “4He+ and 56Fe14+" and “4He+ and 58Ni15+", are generated and characterized successfully. Then, a complex radiation environment is mimicked within the fusion reactors by applying variable-energy irradiation. Secondly, similar penetration depths for different ions, which are crucial for studying synergistic effects, are obtained by precisely controlling the energy of the cocktail beams through the innovative energy modulation system of the LEAF platform. Finally, the post-irradiation analyses, performed by using the transmission electron microscopy (TEM) and nanoindentation, revealed distinct microstructural changes and alterations in material properties, providing insights into the degradation mechanisms under irradiation. This work not only generates diverse and high-intensity “cocktail” ion beams but also achieves rapid energy switching of the beams. Further, the work is expected to pave the way for the implementation of a novel multi-beam irradiation technique in advanced heavy-ion linear accelerators, and also to provide innovative experimental methods and technical support for studying the synergistic effects of nuclear materials.

Updimensioning strategy derived from synthetic equiaxed grain structures for approximating 3D grain size distributions from 2D visualizations with 1D parameters

We generated synthetic equiaxed grain structures using computer graphics software to explore the relationship between various grain size determination methods and true three-dimensional (3D) grain diameters. Mirroring grain measurement techniques, the synthetic 3D grain structures are imaged as 2D micrographs which are measured to yield 1D grain size parameters. Synthetic grain structures provide data at a mass scale and permit exploration of both polished and fractured surface micrographs, revealing one-to-one correspondence between exposed 2D grain cross-sections and individual 3D grains. Analysis of this correspondence yielded a procedure to approximate 3D equiaxed grain size and volume distributions based on the mode of the 2D fractograph grain size distribution. The 3D approximation procedure is shown to be less susceptible to different imaging conditions that affect small, undiscernible grains compared to the standard planimetric and linear intercept methods, which by design also tend to underestimate the 3D grain diameter. The procedure requires larger sample sizes to lower variance and a deeper analysis which could become more practical with machine learning (ML) models for grain boundary segmentation, which synthetic grain structures can help train. This work lays the foundation for analyzing other grain distributions such as columnar and composite grains in similar depth.

Medetomidine-vatinoxan-midazolam provides similar sedation depth with reduced bradycardia compared to dexmedetomidine-midazolam in pigeons (Columba livia domestica).

To determine if sedation with medetomidine-vatinoxan (Zenalpha; Dechra Veterinary Products) and midazolam (Alvogen) (ZM) would cause less cardiovascular depression and maintain similar depth and duration of sedation in pigeons (Columba livia domestica) compared to dexmedetomidine and midazolam (DM). In a blinded crossover study, 15 healthy adult domestic pigeons were sedated IM with either dexmedetomidine (0.08 mg/kg) and midazolam (2 mg/kg) or medetomidine (0.16 mg/kg), vatinoxan (3.2 mg/kg), and midazolam (2 mg/kg) from November through December 2023. Each subject was monitored for 60 minutes, then the sedation was reversed with atipamezole (0.8 mg/kg) and flumazenil (0.1 mg/kg) as needed. Sedation scores, heart rates, and respiratory rates were compared. There was no significant difference in the peak sedation score between DM and ZM groups, with both exhibiting median scores of 4 (heavy sedation). Mean heart rate was significantly higher for ZM than DM at 5, 10, 20, 30, 45, 60, and 65 minutes postinjection. Bradycardia occurred in both groups at 5 and 10 minutes postinjection and persisted for DM until reversal with atipamezole. Arrhythmias were auscultated in both groups. Bradypnea was not observed in either group, and all birds resumed normal behavior following recovery and the following day. Medetomidine-vatinoxan-midazolam provides a similar depth of sedation to DM but with less incidence of bradycardia. Further study is needed to determine the clinical applicability of this sedative in birds. Medetomidine-vatinoxan may be considered for short-term sedation and restraint in cardiovascularly stable pigeons.

Validating a practical methodology for thatch—mat—soil distinction in turfgrass soils

AbstractDiscerning thatch and mat in turfgrass soils is important in organic matter research and choosing the correct turfgrass maintenance actions to keep turfgrass healthy and optimize playability. However, determining boundaries of thatch and mat in practice is often experienced as difficult because of inherent ambiguity in terminology and diagnostic techniques. We described a practical method for the distinction of thatch, mat, and soil layers, being a combination of visually and manually observable characteristics. For two widely different turfgrass species, we analyzed total organic matter (TOM) and dry bulk density (ρd) in thin slices of 6 mm (between 0‐ and 10‐cm soil depth), resulting in clear patterns for both soil properties with increasing depth. Statistical analysis of TOM patterns resulted in similar boundary depths between calculated and observed layers, validating our practical method for the distinction of thatch, mat, and soil layers as a reliable method.

Advanced soil-gas geochemical exploration methods for orogenic gold deposits: A case study of Chalapu deposit, Xizang

Orogenic gold deposits play a significant role in the world’s gold reserves, and their distinctive characteristics pose challenges for exploration. The low sulfide content and narrow, fault-controlled sulfide-quartz veins hinder the use of traditional mineral exploration methods to locate deeply buried ore bodies, particularly in regolith-covered areas. Enhanced methods are required to detect deposits at greater depths. Soil gas composition shows promising potential for mineral exploration by conveying information about sulfur-rich and carbon-rich gases related to deep-seated mineralization into surface soils. However, the prospects of this method for gold deposits remain uncertain. In this study, a novel method was introduced to perform an integrated H2S, SO2, CH4, and CO2 soil-gas geochemical survey on gold ore bodies of different scales at the Chalapu deposit in Tibet. The results unveiled notable gas geochemical anomalies of H2S, SO2, CH4, and CO2 above the multi-layered or thick gold ore bodies. For instance, on exploration line 16 at point A1607, H2S reached the regional maximum value of 1.064 ppm, coinciding with the surface exposure of the thickest gold ore body, measuring 19.81 m. While the presence of CH4 and CO2 anomalies alone may not signify potential mineralized zones, the combined presence of anomalous H2S and SO2 values along with CH4 and CO2 concentrations appears to be effective in identifying mineralization. These combinations of geochemical anomalies not only uncover concealed ore bodies, but also delineate the trend and extension direction in strike and dip of ore bodies. Gas intensity may provide insights into the scale of the ore bodies, with stronger gas anomalies observed in areas with thicker ore bodies at similar depths. Thus, the study reveals that soil-gas exploration shows great promise as an exploration technique for orogenic gold deposits, especially in areas with regolith cover hindering traditional methods from detecting mineralized zones for gold exploration.

From melt- to crystal-rich magmatic systems during rift localization: Insights from mineral chemistry in Central Afar (Ethiopia)

Magmatism plays a key role in accommodating and localizing extension during continental breakup. However, how the crustal magmatic systems evolve at the continental-ocean transition is poorly understood. We address these questions by studying the evolution of the magmatic system in the rift of Central Afar (Ethiopia), currently marking the transition from continental rifting to oceanic spreading. We focus on the voluminous and widespread Upper Stratoid Series (2.6–1.1 Ma) and the following Central Afar Gulf Series (1.1–0.6 Ma), the latter corresponding to localization of volcanism in narrow magmatic segments. We carried out the first systematic study of major and trace element mineral chemistry for these two Series and integrated it with geothermobarometry estimates and geochemical modeling, to reconstruct the evolution of the magmatic system architecture during rift localization. The Upper Stratoid magmas evolved by fractional crystallization in a melt-rich, moderately zoned, middle-lower crustal (10–18 km) magmatic system, from where they rose directly to the surface. Polybaric plagioclase convection and dissolution of a plagioclase-rich crystal mush is recorded in the phenocryst texture and chemistry. The Central Afar Gulf magmas evolved at similar depth in a more complex and dynamic storage system, with magma rising and mixing through multiple, relatively small, crystal-rich and interconnected reservoirs. Our study documents the transition during the continental breakup, from an overall stable and melt-rich magmatic system feeding the voluminous and homogeneous Upper Stratoid eruptions to a more dynamic, interconnected and crystal-rich situation feeding small-volume eruption while the rift localizes.

Large Eddy Simulation of Cross‐Shore Hydrodynamics Under Random Waves in the Inner Surf and Swash Zones

AbstractA 3D large eddy simulation coupled with a free surface tracking scheme was used to simulate cross‐shore hydrodynamics as observed in a large wave flume experiment. The primary objective was to enhance the understanding of wave‐backwash interactions and the implications for observed morphodynamics. Two simulation cases were carried out to elucidate key processes of wave‐backwash interactions across two distinct stages: berm erosion and sandbar formation, during the early portion of a modeled storm. The major difference between the two cases was the bathymetry: one featuring a berm without a sandbar (Case I), and the other, featuring a sandbar without a berm (Case II) at similar water depth. Good agreement (overall Willmott's index of agreement greater than 0.8) between simulations and measured data in free surface elevation, wave spectrum, and flow velocities validated the model skill. The findings indicated that the bottom shear stress, represented by the Shields parameter, was significant in both cases, potentially contributing substantial sediment transport. Notably, the occurrence of intense wave‐backwash interactions were more frequent in the absence of a sandbar. These intense wave‐backwash interactions resulted in a pronounced horizontal pressure gradient, quantified by high Sleath parameters, exceeding the criteria for momentary bed failure. Additionally, a more vigorous turbulence‐bed interaction, characterized by near‐bed turbulent kinetic energy, was observed in the case lacking a sandbar, potentially augmenting sediment suspension. These insights are pivotal in understanding the mechanisms underlying berm erosion and how sandbar formation serves to protect further beach erosion.

The K s -band Luminosity Function of the Rich Cluster VC04 in the Vela Supercluster

To learn more about the properties of the Vela Supercluster (VSCL), located behind the Milky Way at cz ∼ 18,000 km s−1, we determine the K s -band luminosity function (LF) of VC04, the richest known galaxy cluster in the VSCL, and two other VSCL clusters (VC02 and VC08). The galaxy sample is based on NIR observations that are complete to an extinction-corrected absolute magnitude of MKso<−21.ͫ5 (∼2.ͫ5 below MK* ), within the clustercentric radius of r c < 1.5 Mpc (∼70% of the Abell radius). For VC04, we obtained 90 new spectroscopic redshifts of galaxies, observed with the 11 m Southern African Large Telescope. We found the Schechter parameters of the VC04 LF to be M* = −24.ͫ41 ± 0.44, α = −1.10 ± 0.20, and ϕ* = 8.84 ± 0.20. Both the redshift data and the LF confirm VC04 to be a rich but not yet fully relaxed cluster. We independently determined the LF of VC04 on membership defined by the red-sequence method and demonstrated that this method can be used in the absence of high spectroscopic coverage over a cluster. This allowed us to determine the LFs of VC02 and VC08. We also derived the LFs of the Coma, Norma, and Virgo clusters to similar depth and extent as the VSCL clusters. We found that the Schechter parameters of VC04 are within 1σ uncertainties of these local clusters, as well as VC02 and VC08. We do not find significant differences between the LFs in the different cluster environments probed in this work down to MK*+2.ͫ5 .

Co-seismic and post-seismic slip associated with the 2021 Mw5.9 Arkalochori, Central Crete (Greece) earthquake constrained by geodetic data and aftershocks

The co-seismic and post-seismic deformation field associated with the Mw5.9 Arkalochori main shock that occurred in central Crete (Greece) on 27 September 2021 is analyzed using Copernicus Sentinel-1A & 1B images, GNSS measurements and seismological data. The fault geometry is constrained through the joint inversion of multiple datasets and the slip distribution for the co-seismic and post-seismic period is obtained using a homogeneous half-space elastic model and the Steepest Descent Method. The results indicate a blind normal fault striking 215° with a 55° dip to the northwest and the co-seismic slip model suggests a nearly circular main slip patch (8 × 6 km2) with a maximum slip of 0.98 m. Post-seismic displacements started rapidly after the main shock followed by a gradual decay as highlighted by the calculated InSAR time series. The temporal evolution of post-seismic slip is described by a simple logarithmic function, decaying faster at the southwest part of the fault. The cumulative afterslip model suggests that the maximum post-seismic slip of 0.23 m occurred within a similar depth range compared to the co-seismic one, yet with a shift towards the southwest. Post-seismic slip inside the main shock rupture area is sustained, highlighting the slow recovery of locking in the co-seismic slip region. Afterslip (seismic or aseismic) played a dominant role in the early post-seismic period acting complementarily to the main rupture. Indications suggest that the spatiotemporal evolution of the productive aftershock sequence may be driven afterslip, alongside other potential factors.

A diversity baseline of benthic macrofauna along the northwestern slope of Cuba (Gulf of Mexico)

The Gulf of Mexico (GoM) is a unique ecosystem due to its physical characteristics, being influenced by the Mississippi River in the north and the Loop Current from the south, resulting in a gradient of organic to carbonate sediment composition from north to south. The continental slope of the northern and southwestern portions of the GoM are generally well studied; however, less is known about the southeastern GoM along the slope of Cuba. To fill this knowledge gap, sediment cores were collected in 2017 at nine stations (974–1580 m depth) to determine abiotic controls on the deep-sea benthic macrofauna community. Oceanographic data indicated a stratified water column typical of an oligotrophic ocean and no evidence of hypoxia. Sediment texture and composition indicated a west-east gradient likely determined by downslope transport of terrigenous material in the eastern part with a high proportion of carbonate in the west. Heavy metals (Cu, Hg, Pb, and Zn) at concentrations known to cause adverse benthic effects were present in the east near the city of Havana, with the macrofauna community showing characteristics indicative of environmental stress. Overall, this region supported a diverse community of macrofauna families of low abundance, typically only 1–2 animals, and high variability among replicates within stations. Rarefaction curves revealed higher biodiversity per number of individuals in the samples from Cuba compared to those from the nGoM at similar depths, though more samples would be needed to better reveal the true diversity. The major factors influencing macrofauna communities in the continental slope off northwestern Cuba are most likely the lack of organic-rich sediment and low sediment deposition rates, both of which can be attributed to the strong currents and lack of major terrigenous input, along with the regular natural disturbances which prevents domination.

Mars' North Polar Spiral Trough Migration Paths as Revealed Through 3D Radar Mapping

AbstractWe present observations and maps of Mars' northern polar spiral trough migration paths (TMPs) as revealed through the 3D SHARAD (Shallow Radar) data volume, identify patterns of TMP morphology, and provide interpretations and implications of these TMPs on past climate. This data set provides additional spatial context over traditional 2D data as it allows for radargrams to be taken perpendicular to each trough strike, which minimizes distortion of migration paths due to oblique viewing geometry. Here, we present an expanded survey of trough migration and analysis of surface troughs across the north polar layered deposits. We corroborate many previous observations of trough migration such as: the general poleward and stratigraphically upward migration from the TMP initiation point; the broadly similar depth of trough initiation across the cap; regional variability in TMP morphology; and the presence of buried promontories. Our results support the presence of two generations of troughs, though we cannot confirm that the oldest generation originated deeper than ∼600 m beneath the current surface, as previously reported. Two generations of troughs suggest that climate conditions favorable for initiation of trough migration have occurred at least twice. Trough initiation occurring only in the upper ∼600 m suggests that troughs are relatively recent features in the polar cap, developing after the formation of ∼half the current cap height. The mapped TMPs provide insight into the consistency of Mars' climate during the accumulation of the past few hundred meters of ice by recording the boundary of erosion and accumulation of ice at the troughs.

Field comparison of Antarctic krill (Euphausia superba) backscatter and aggregation types using NORTEK and SIMRAD echosounders

Abstract Temporal distributions of Antarctic krill (Euphausia superba) density and aggregation types were characterized and compared using Nortek Signature100 and SIMRAD Wideband Autonomous Transceiver (WBAT) upward-looking echosounders. Noise varied between the two echosounders. With the Signature100, it was necessary to correct data for background, transient, and impulse noises, while the WBAT data needed to be corrected for background noise only. For selected regions with no visible backscatter, the signal-to-noise ratio of Sv values (i.e. the ratio between the signal and the background noise level) did not vary between the two echosounders. Surface echo backscatter was similar during similar time periods. Descriptive metrics were used to quantify spatial and temporal krill vertical distributions: volume backscatter, mean depth, center of mass, inertia, equivalent area, aggregation index, and proportion occupied. Krill backscatter density differed between the two instruments but was detected at similar mean depths. Krill aggregations were identified at each mooring location and classified in three types based on morphological characteristics. Each type of aggregation shape differed at the two spatially separated moorings, while the acoustic density of each aggregation type was similar. The Signature100 detected a lower number of krill aggregations (n = 133) compared to the WBAT (n = 707). Although both instruments can be used for autonomous deployment and sampling of krill over extended periods, there is a strong caveat for the use of the Signature100 due to significant differences in noise characteristics and krill detection.

Analysis of DNA from brain tissue on stereo-EEG electrodes reveals mosaic epilepsy-related variants.

Somatic mosaic variants contribute to focal epilepsy, but genetic analysis has been limited to patients with drug-resistant epilepsy (DRE) who undergo surgical resection, as the variants are mainly brain-limited. Stereoelectroencephalography (sEEG) has become part of the evaluation for many patients with focal DRE, and sEEG electrodes provide a potential source of small amounts of brain-derived DNA. We aimed to identify, validate, and assess the distribution of potentially clinically relevant mosaic variants in DNA extracted from trace brain tissue on individual sEEG electrodes. We enrolled a prospective cohort of eleven pediatric patients with DRE who had sEEG electrodes implanted for invasive monitoring, one of whom was previously reported. We extracted unamplified DNA from the trace brain tissue on each sEEG electrode and also performed whole-genome amplification for each sample. We extracted DNA from resected brain tissue and blood/saliva samples where available. We performed deep panel and exome sequencing on a subset of samples from each case and analysis for potentially clinically relevant candidate germline and mosaic variants. We validated candidate mosaic variants using amplicon sequencing and assessed the variant allele fraction (VAF) in amplified and unamplified electrode-derived DNA and across electrodes. We extracted DNA from >150 individual electrodes from 11 individuals and obtained higher concentrations of whole-genome amplified vs unamplified DNA. Immunohistochemistry confirmed the presence of neurons in the brain tissue on electrodes. Deep sequencing and analysis demonstrated similar depth of coverage between amplified and unamplified samples but significantly more called mosaic variants in amplified samples. In addition to the mosaic PIK3CA variant detected in a previously reported case from our group, we identified and validated four potentially clinically relevant mosaic variants in electrode-derived DNA in three patients who underwent laser ablation and did not have resected brain tissue samples available. The variants were detected in both amplified and unamplified electrode-derived DNA, with higher VAFs observed in DNA from electrodes in closest proximity to the electrical seizure focus in some cases. This study demonstrates that mosaic variants can be identified and validated from DNA extracted from trace brain tissue on individual sEEG electrodes in patients with drug-resistant focal epilepsy and in both amplified and unamplified electrode-derived DNA samples. Our findings support a relationship between the extent of regional genetic abnormality and electrophysiology, and suggest that with further optimization, this minimally invasive diagnostic approach holds promise for advancing precision medicine for patients with DRE as part of the surgical evaluation.

The importance of perinatal non-birthing parents’ mental health and involvement for family health

Perinatal mental health research typically focuses on the birthing parent's experiences and their influence on birth/child outcomes, while not considering non-birthing parents in similar depth. Non-birthing parents are also at increased risk for mental illness during the perinatal period, and non-birthing parents’ health and involvement affect the health of birthing people, fetuses, and newborns, necessitating greater understanding of non-birthing parents’ contributions to family functioning. This review examines perinatal mental health disorders in non-birthing parents, their relationship quality with the birthing parent, and how the non-birthing parent's mental health and involvement affects the health outcomes of the birthing parent and the child. Recommendations are provided for healthcare professionals who work with perinatal patients and their families to engage non-birthing parents, learn about non-birthing parent health, and facilitate connections to care. By doing so, professionals working with perinatal patients can optimize health outcomes for their patients and the family as a whole.