Similar Chemical Profiles Research Articles

Comparative transcriptome profiling reveals distinct regulatory responses of secondary defensive metabolism in Datura species (Solanaceae) under plant development and herbivory-mediated stress.

Differential expression of genes is key to mediating developmental and stress-related plant responses. Here, we addressed the regulation of plant metabolic responses to biotic stress and the developmental variation of defense-related genes in four species of the genus Datura with variable patterns of metabolite accumulation and development. We combine transcriptome profiling with phylogenomic techniques to analyze gene expression and coexpression in plants subjected to damage by a specialist folivore insect. We found (1) common overall gene expression in species of similar chemical profiles, (2) species-specific responses of proteins involved in specialized metabolism, characterized by constant levels of gene expression coupled with transcriptional rearrangement, and (3) induction of transcriptional rearrangement of major terpene and tropane alkaloid genes upon herbivory. Our results indicate differential modulation of terpene and tropane metabolism linked to jasmonate signaling and specific transcription factors to regulate developmental variation and stress programs, and suggest plastic adaptive responses to cope with herbivory. The transcriptional profiles of specialized metabolism shown here reveal complex genetic control of plant metabolism and contribute to understanding the molecular basis of adaptations and the physiological variation of significant ecological traits.

Serum metabolic profiling in pregnant Holstein cows 3 weeks prior to parturition using two-dimensional gas chromatography time-of-flight mass spectrometry.

This preliminary study explored potential serum biomarkers for predicting the onset of milk fever (MF), a bovine parturient disease with hypocalcemia. We conducted two-dimensional gas chromatography mass spectrometry-based metabolomics in 8 and 17 pregnant Holstein cows that did and did not develop MF 3 weeks later, respectively. In principal component analysis (PCA) applied to a dataset containing 1,498 metabolites, serum metabolites exhibited highly similar chemical profiles between cows with and without MF. PCA with a limited dataset of metabolites containing fatty acids, which had significantly different values between the groups and/or correlation coefficients of >0.5 for the serum calcium concentration, distinguished the two groups. These suggest the possibility of developing serum biomarkers for predicting bovine MF.

Further Insights on Honey and Propolis from Gerês (Portugal) and Their Bioactivities: Unraveling the Impact of Beehive Relocation.

Propolis, a bee product, is known for its variability of chemical and bioactive profiles. However, Portuguese propolis from Gerês, normally obtained by mixing propolis from three places-Bugalho, Felgueiras and Toutelo-has shown similar chemical and biological profiles over the years. Recently, a new propolis place-Roca-was added to the apiary to replace Bugalho, lost to the 2017 wildfires, hence questioning the previously claimed constancy of Gerês propolis. To unravel to what extent the beehive relocation affected this constancy, we studied different Gerês propolis samples collected in three consecutive years (2017-2019) composed of different combinations of source places. Two honey samples, collected before (2017) and after (2018) the occurrence of the wildfire, were also investigated. Total phenolics, flavonoids and ortho-diphenols contents were determined and the antioxidant and antimicrobial activities were evaluated, using the DPPH assay and the agar dilution method, respectively. Although both antimicrobial and antioxidant activities were generally in the ranges usually obtained from Gerês propolis, some variations were detected for the samples, with different compositions when compared to previous years. This work reinforces the importance of the consistency of a combination of several factors for the protection and preservation of the flora near the hives, providing bee products with more constant chemical and biological profiles over the years.

Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation

Bacterial nanocellulose (BNC) is a remarkable biopolymer synthesised by bacterium, exhibiting exceptional properties. However, conventional Hestrin-Schramm (HS) medium, particularly the carbon source, poses challenges of high costs and low productivity. This study explores BNC biosynthesis on a modified HS medium, employing agricultural wastes (sugarcane molasses, banana peel, and pineapple peel) as carbon sources, and compares the overall yield of BNC produced. Sugarcane molasses proved to be the most effective, yielding the highest BNC concentration (8.19 g/L) after 7 days, followed by pineapple peel (2.16 g/L) and banana peel (2.11 g/L). Extensive research was conducted to enhance properties of BNC by an environmentally friendly approach, incorporating silver nanoparticles (AgNP) utilising Momordica charantia fruit extract, resulting in a BNC-Ag nanocomposite. The synthesis involved mixing 1 mM silver nitrate (AgNO3) with 15 mL of M. charantia fruit extract to reduce Ag ions to AgNP, which was confirmed by UV-vis spectroscopy with an absorbance peak between 400 and 410 nm. Characterisation using FESEM and TEM on the synthesized BNC showed minimal impact on BNC fiber diameter from waste-derived carbon sources. XRD indicated slight variations in crystallinity index, with the highest (85%) in TSM-derived BNC. FTIR analysis revealed similar chemical profiles across all BNC, indicating cellulose formation. The BNC-Ag nanocomposite exhibited potent antibacterial activity against multi-drug resistant strains (Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtilis, Staphylococcus aureus) through disc diffusion method with inhibition zones up to 16.8 mm. Overall, the findings from this study contribute to the development of environmentally sustainable for the production of functional BNC materials with enhanced properties for diverse applications.

NMR Metabolomics and Chemometrics of Commercial Varieties of Phaseolus vulgaris L. Seeds from Italy and In Vitro Antioxidant and Antifungal Activity.

The metabolite fingerprinting of four Italian commercial bean seed cultivars, i.e., Phaseolus Cannellino (PCANN), Controne (PCON), Vellutina (PVEL), and Occhio Nero (PON), were investigated by Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate data analysis. The hydroalcoholic and organic extract analysis disclosed more than 32 metabolites from various classes, i.e., carbohydrates, amino acids, organic acids, nucleosides, alkaloids, and fatty acids. PVEL, PCON, and PCANN varieties displayed similar chemical profiles, albeit with somewhat different quantitative results. The PON metabolite composition was slightly different from the others; it lacked GABA and pipecolic acid, featured a higher percentage of malic acid than the other samples, and showed quantitative variations of several metabolites. The lipophilic extracts from all four cultivars demonstrated the presence of omega-3 and omega-6 unsaturated fatty acids. After the determination of the total phenolic, flavonoids, and condensed tannins content, in vitro antioxidant activity was then assessed using the DPPH scavenging activity, the ABTS scavenging assay, and ferric-reducing antioxidant power (FRAP). Compared to non-dark seeds (PCON, PCANN), brown seeds (PVEL, PON) featured a higher antioxidant capacity. Lastly, only PON extract showed in vitro antifungal activity against the sclerotia growth of S. rolfsii, by inhibiting halo growth by 75%.

Identification of Acetomycin as an Antifungal Agent Produced by Termite Gut-Associated Streptomycetes against Pyrrhoderma noxium.

Plant fungal pathogen Pyrrhoderma noxium is responsible for the destructive and invasive disease of brown root rot currently affecting the city of Brisbane, Australia. In order to address this issue, environmentally friendly and safe alternatives to chemical control are preferred due to the city's public setting. Antifungal natural products are ideal candidates as biological control alternatives and can be detected through investigating the metabolomes of microbial symbionts. Within this study, an NMR-based metabolomics approach was applied to fermentation extracts obtained from 15 termite gut-associated streptomycetes. By analysing the NMR spectra, six of the extracts which displayed similar chemical profiles exhibited antifungal activity against the P. noxium pathogen. The major compound within these extracts was identified as acetomycin using NMR and X-ray crystallography analyses. This is the first reporting of acetomycin as a potential natural product fungicide, particularly as an antifungal agent against P. noxium. Inhibitory activity was also found against other important fungal crop pathogens, including Aspergillus niger, Botrytis cinerea, and Alteranaria alternata. Further experimentation using a woodblock test found inhibitory activity on the growth of the P. noxium pathogen for up to 3 weeks and a significant difference in the integrity of the woodblocks when conducting compression strength tests after 6 weeks. Therefore, acetomycin may be used as a biological control agent and natural product fungicide against P. noxium.

Uticaj klimatskih faktora na akumulaciju isparljivih komponenti u etarskom ulju i hidrolatu Salvia officinalis

The study investigated the cultivation, chemical composition, and potential applications of Salvia officinalis L. cultivar "Primorska" over three years. The research revealed that the essential oil of S. officinalis contains significant amounts of oxygenated monoterpenes, including a-thujone (19.9-29.0%), camphor (15.8-19.6%), b-thujone (12.3-13.3%), and 1,8-cineole (8.8-11.3%). The content of these compounds varied across years and correlated with temperature fluctuations. The hydrolate derived from S. officinalis exhibited a similar chemical profile to the essential oil, with camphor emerging as the most abundant compound (42.4-49.6%). Statistical analysis revealed correlations between temperature, precipitation, and the content of various compounds in both the essential oil and hydrolate. Temperatures are in strong negative correlation with a-thujone (KT=-36.500), and in positive correlation with camphor (KT=9.5), b-thujone (KT=4.500) and 1,8-cineole (KT=5.000) in essential oil. In hydrolates is also noted similar trend of correlations: negative correlations between temperature and a-thujone (KT=-32.000), and positive correlations temperature and camphor (KT=34.000), b-thujone (KT=7.000), and 1,8-cineole (KT=7.000). S. officinalis essential oil is highly valued in the food and pharmaceutical industry, as well as in cosmetics, perfumery and aromatherapy. Numerous studies have indicated its antioxidant, antimicrobial, anti-inflammatory and anticancer potential. The study also highlighted potential applications of S. officinalis hydrolate in cosmetics, food sanitation, and pharmaceuticals due to its antioxidant properties and moderate antimicrobial effects. Overall, the research contributes to a deeper understanding of S. officinalis products' chemical composition and underscores the potential of its hydrolate as a valuable resource in multiple industries. Further exploration into its applications and benefits will drive innovation in product development and utilization.

Examination of Raw Samples and Ethanol Extracts of Gerês Propolis Collected in Different Years.

Propolis, a natural resin created by bees, has garnered significant attention from both the scientific community and industry due to an impressive range of bioactivities. Nonetheless, the intrinsic variability in its chemical composition and bioactive profiles has been hindering propolis' full potential use. We previously showed that ethanol extracts (EEs) of a Portuguese propolis sample (Gerês) collected over four consecutive years displayed similar chemical and biological profiles, a constancy never documented before. However, the characteristics of the unprocessed samples of Gerês propolis were never described. Hence, the central objective of this study is to assess the quality parameters of unprocessed propolis samples collected from Gerês (G), over a four-year period (2019-2022), alongside the analysis of the chemical composition and bioactivities of the EEs prepared with the same raw samples. The ash, wax, balsam and water contents of the unprocessed samples-G19 to G22-showed minor fluctuations, likely attributed to uncontrollable natural events impacting the propolis source and collection process. On the other hand, the antimicrobial and antioxidant activities of all the four ethanol extracts (G19.EE-G22.EE) consistently align with prior studies. Furthermore, the Gerês propolis extracts showed remarkable uniformity in chemical composition parameters too, particularly concerning total polyphenol, flavonoid and ortho-diphenol contents. In summary, our research reinforces the beneficial properties of propolis and show that extracts' bioactivities remain within the reference ranges for Gerês propolis, despite minor differences in unprocessed samples, suggesting a consistent action over time. Thus, this work could be instrumental towards the establishment of standard parameters for propolis applications, offering valuable insights to this field of propolis research.

Chemical element distribution in Arctic soils: Assessing vertical, spatial, animal and anthropogenic influences in Elsa and Ebba Valleys, Spitsbergen, Svalbard

The Arctic region is threatened by climate change and pollution caused by human activities which potentially influence the elemental concentrations available to and from the biota. To better understand this delicate balance, it is crucial to investigate the role of several factors. Therefore, we quantified the level of 43 chemical elements in soils from Elsa and Ebba Valleys, Petunia Bay, Spitsbergen, a region that has experienced lasting environmental impacts from historical mining activities. We evaluated the a) vertical sampling influence by examining the variation in element distribution between the soil upper and deeper layers, b) animal influence by verifying the role of native animals, particularly vertebrates, in introducing thought faeces elements to the soil and c) anthropogenic influence by studying the spatial geographical differences in element distribution based on the degree of human pressure between the valleys. Our analysis also includes data on soil organic matter (SOM) and mineral composition. Both valleys exhibited similar mineralogical composition, but Elsa Valley had higher concentrations of most analyzed elements compared to Ebba Valley. Despite the contribution of vertebrate feaces, no increase in element concentrations was observed in the animal-influenced soils. The sampled soil layers had similar chemical element profiles for most elements. SOM content tended to be higher in superficial soils and showed a strong positive correlation with most quantified elements. The higher concentrations in Elsa Valley reflect its past mining and mineral exploration, making this area more significantly impacted than Ebba Valley. Surprisingly, vertebrate animals do not appear to influence the concentrations of chemical elements or organic matter in soils. Our findings provide valuable insights into the legacy of past mining activities and mechanisms driving environmental change in the Arctic.

Jintiange proteins promote osteogenesis and inhibit apoptosis of osteoblasts by enhancing autophagy via PI3K/AKT and ER stress pathways.

Tiger bone, which had long been used in traditional Chinese medicine, had the action of removing wind and alleviating pain, strengthening the sinews and bones, and often used to treat bone impediment, and atrophic debility of bones in TCM clinical practice. As a substitute of natural bone tiger, artificial tiger bone Jintiange (JTG), has been approved by the State Food and Drug Administration of China for relief the symptom of osteoporosis, such as lumbago and back pain, lassitude in loin and legs, flaccidity and weakness legs, and walk with difficulty based on TCM theory. JTG has similar chemical profile to natural tiger bone, and contains mineral substance, peptides and proteins, and has been shown to protect bone loss in ovariectomized mice and exert the regulatory effects on osteoblast and osteoclast activities. But how the peptides and proteins in JTG modulate bone formation remains unclear. To investigate the stimulating effects of JTG proteins on osteogenesis and explore the possible underlying mechanisms. JTG proteins were prepared from JTG Capsules by extracting calcium, phosphorus and other inorganic elements using SEP-PaktC18 desalting column. MC3T3-E1 cells were treated with JTG proteins to evaluate their effects and explore the underlying mechanisms. Osteoblast proliferation was detected by CCK-8 method. ALP activity was detected using a relevant assay kit, and bone mineralized nodules were stained with alizarin red-Tris-HCl solution. Cell apoptosis was analyzed by flow cytometry. Autophagy was observed by MDC staining, and autophagosomes were observed by TEM. Nuclear translocations of LC3 and CHOP were detected by immunofluorescence and observed under a laser confocal microscope. The expression of key proteins related to osteogenesis, apoptosis, autophagy and PI3K/AKT and ER stress pathways was analyzed by Western Blot analysis. JTG proteins improved osteogenesis as evidenced by the alteration of proliferation, differentiation and mineralization of MC3T3-E1 osteoblasts, inhibited their apoptosis, and enhanced autophagosome formation and autophagy. They also regulated the expression of key proteins of PI3K/AKT and ER stress pathways. In addition, PI3K/AKT and ER stress pathway inhibitors could reverse the regulatory effects of JTG proteins on osteogenesis, apoptosis, autophagy and PI3K/AKT and ER stress pathways. JTG proteins increased the osteogenesis and inhibited osteoblast apoptosis by enhancing autophagy via PI3K/AKT and ER stress signaling pathways.

Preen oil composition of Pied Flycatchers is similar between partners but differs between sexes and breeding stages

Preen oil, the secretion of the uropygial gland, may be an important source of body odour in birds. By characterizing the chemical composition of preen oil, we can describe the olfactory phenotypes of birds and investigate whether odours could have a function in sexual signalling or other chemical communication. Here we analysed the preen oil of a wild passerine, the European Pied Flycatcher Ficedula hypoleuca, to find out whether it holds socially relevant information. We sampled both the female and male of breeding pairs during nestling rearing to test for sex differences and within‐pair similarity. We additionally sampled the females during incubation to test for changes across breeding stages and for individual repeatability of chemical profiles. Pair mates had similar chemical profiles in comparison with other breeding adults. Furthermore, we found evidence for sex differences and for changes across breeding stages. Notably, the preen oil of females was more diverse and more volatile than that of males, and the preen oil secreted by females during incubation was more volatile than that secreted during nestling rearing. However, we found no evidence for individual repeatability of chemical profiles across breeding stages in females. Our results point towards a function of preen oil in sexual signalling, although other functions should not be excluded. Our study is a first step towards understanding the role of odours in the social life of an important avian model species used in the study of mate choice and sexual selection.

Sensomic comparison of lager beers fermented by selected Saccharomyces pastorianus yeast strains

Selection of the appropriate yeast strain is one of the most crucial steps in a brewery. Traditionally, yeast strain's abilities during beer fermentation are described according to brewer's experiences. Hence, these descriptions could be inaccurate and strictly based on sensory experiences. In this study, lager beers fermented by four traditional bottom-fermented yeast strains were characterized in detail by sensomic approach. The obtained results revealed that yeast strains can influence most of the sensory-related components in beer, not only esters and higher alcohols, but also carbonyls, amino acids, saccharides, fatty acids, heterocyclic compounds, hop oils, and other hop-related components. By comparison of chemical and sensory characteristics of each studied beer, the theoretical importance of sensory interactions on beer flavor perception was also revealed as the general conception that the beers with similar flavors have also similar chemical profiles (and vice versa) was seemed as not valid.

A novel approach for improved honey identification and scientific definition: a case of buckwheat honey

This study aimed to provide insights into a novel honey screening and authentication approach. Chemometrics associated with a GC/MS instrumentation was applied to verify a total of 13 honey samples having different floral and geographical origin with an aim to detect buckwheat honey and discriminate it from honeys of other floral sources. Non-polar and semi-polar compounds were first extracted and then detected and semi-quantified employing a GC/MS device working in a full scan mode. Fingerprinting signals covering peaks that elute in the first 20 min, comprising 2000 scans of semi-polar compounds, were used as input datasets for unsupervised and supervised chemometric tools. This approach demonstrated excellent classification performances of honey samples according to the declared floral source, regardless of their geographical origin. The obtained results were compared with the outputs of a common melissopalynological procedure. Although exhibiting a high variability in the pollen content, samples declared as buckwheat honey demonstrated similar chemical profiles. The proposed non-targeted and semi-quantitative method showed to be rapid, unbiased, independent of tedious qualitative and quantitative determinations of eluting compounds, thus exhibiting a strong potential to be incorporated into data fusion procedures for honey authentication. Harmonising chemical methodologies with certain indications from pollen analysis to form core-data systems would undoubtedly improve the authentication protocols of honey floral sources.

Thermal Treatment and High-Intensity Ultrasound Processing to Evaluate the Chemical Profile and Antioxidant Activity of Amazon Fig Juices

The present paper evaluated the influence of heat treatment (HT) and high-intensity ultrasound (HIUS) on the chemical profile of the Amazon fig (Ficus subapiculata, Moraceae) juices. Antioxidant activity, quantification of carotenoids, total phenolic compounds (TPC), pH, titratable acidity, soluble solids, color and chemical profile (NMR) were evaluated. Treatments did not change the pH (3.4–3.5), titratable acidity (0.044–0.048%) and soluble solids (2.3–2.4 °Brix). The highest antioxidant activity (DPPH, ABTS) and TPC were presented by the HT-treated juice, which was equivalent to 1235 ± 11 µM TE, 1440 ± 13 µM TE and 312 ± 5 mg GAE mL−1, respectively. The treatments influenced the color luminosity according to the L* and a* parameters, while the b* parameter showed no significant change. The L* parameter was elevated in all treated samples compared to the control sample. Analyzing the parameter a* f, it was verified that the sample with thermal treatment (HT) was different from the control sample, but presented similarity with the samples of the HIUS processes. The 1H NMR spectra of the juices showed similar chemical profiles in all treatments. The compounds α-glucose, β-glucose, fructose, citric, malic, quinic, and p-hydroxybenzoic acids were identified. The HT treatment presented higher efficiency to extract the antioxidant compounds from fig juices. The HIUS treatments with constant energy density also improved the tolerance of the antioxidant compounds, especially in conditions of higher potency and reduced time. Future studies will be devoted to carry out microbiological analysis and evaluate the stability of treated juices.

Phytochemical and Biological Study of Trophic Interaction between Pseudosphinx Tetrio L. Larvae and Allamanda Cathartica L.

In this article, we propose to explore the chemical interaction between Pseudosphinx tetrio L. and Allamanda cathartica L. using different analytical methods, including an innovative electrochemical approach (called electrochemical ecology) and multivariate analysis, and we investigate the potential antimicrobial effects (antibacterial and antifungal activities) of this interaction in order to gain a better understanding of their specific interaction. The analytical study presents a similar chemical profile between the leaves of healthy and herbivorous A. cathartica and the excretions of the caterpillars. The similar analytical profile of the leaves of A. cathartica and the excretions of P. tetrio, and the difference with the caterpillar bodies, suggests a selective excretion of compounds by the caterpillar. The measured antimicrobial activities support the physicochemical tests. The natural products found selectively in the excretions (rather than in the body) could explain the ability of P. tetrio to feed on this toxic Apocynaceae species.

Resilience of Stevia rebaudiana (Bertoni) Bertoni in the Underwater Biospheres of Nemo’s Garden®: Adaptation to New Cultivation Systems

The Nemo's Garden® project is an alternative production system for areas with scarce cultivable land but significant presence of water; thus, it is an interesting intervention to address the climate crisis. This work aimed to evaluate the micromorphological, biochemical, and phytochemical characteristics of Stevia rebaudiana (Bertoni) Bertoni grown underwater compared to the terrestrial specimens. The micromorphological analyses, performed on the leaves using light microscopy, fluorescence microscopy, and scanning electron microscopy, evidenced a general uniformity of the trichome morphotype and distribution pattern. The histochemical investigation indicated the simultaneous presence of terpenes and polyphenols in the trichome secreted material from the underwater samples and a prevailing polyphenolic content in the terrestrial specimens; this was also confirmed by biochemical analyses (26.6 mg GAE/g DW). The characterization of non-volatile components, performed using HPLC-MS, showed similar chemical profiles in all the samples, which were characterized by phenolic compounds and steviol glycosides. The volatile compounds, evaluated using HS-SPME coupled with GC-MS, showed sesquiterpene hydrocarbons as the main class in all the analyzed samples (80.1-93.9%). However, the control plants were characterized by a higher content of monoterpene hydrocarbons (12.1%). The underwater biosphere environment did not alter S. rebaudiana micro-morphological characters, although slight qualitative changes were evidenced for the compounds produced as a response to the growth conditions.

Identification of markers for tea authenticity assessment: Non-targeted metabolomics of highly similar oolong tea cultivars (Camellia sinensis var. sinensis)

Tea authenticity assessment remains a challenge because of various cultivars and similar chemical profiles among tea cultivars. Unique oolong tea cultivars (YaShiXiang (YSX)) were used as models in the present study to identify potential markers for tea authenticity assessment via non-targeted metabolomics. With chemometric analysis, a total of ten markers were found to differ significantly among YSX and the high similarity cultivar tea samples, which were up-regulated in YSX. Based on the ten markers, classification model for discrimination of YSX were developed with different combinations of markers. All models were able to classify YSX samples correctly (93.0%–100% for internal cross-validation; 80.5%–99.0% for external validation). The simultaneous determination of metabolites in this study suggested that a combination of non-targeted metabolomics and chemometric methods could provide high resolution markers for tea authenticity assessment, and which can serve as a basis for further quality evaluation and breeding of tea cultivars in the future. • Highly similar oolong tea cultivars were analyzed for tea authenticity assessment. • Chemical composition of tea were analyzed by UHPLC Q-Exative Orbitrap MS. • Ten markers were identified to evaluate the authenticity of oolong tea.

The distinct fruit size and physical defense promote divergent secondary seed dispersal strategies of three oak species

Secondary seed dispersal is one of the most important factors contributing to the co-existence of plant species. Variations in fruit properties including fruit size, physical defense and chemical composition influence seed foraging and secondary dispersal, but the details remain unclear. Here, we focused on three common nut-bearing tree species (Quercus aliena Blume, Q. dentata Thunb., and Q. variabilis Blume), which often co-occur in mixed deciduous forest in northern China. We estimated their acorn properties and evaluated their effects on the secondary dispersal patterns. We found similar chemical profiles but different acorn sizes and physical defense among these three species. Moreover, we detected distinct secondary seed dispersal strategies in relation to their acorn properties: Q. dentata produced the smallest-sized acorns with the lowest physical defense, which had shorter dispersal range but the highest survival rate; Q. variabilis generated the largest-sized acorns with the highest physical defense, which suffered the lowest survival rate but benefited from the longer dispersal range; and an intermediate survival rate existed in Q. alienawith the intermediate-sized acorns. In addition, Père David’s rock squirrel (Sciurotamias davidianus Milne-Edwards, Sciuridae) and brown rat (Rattus norvegicus Berkenhout, Muridae) are secondary seed dispersal agents for three oak species. Brown rats not only disperse more acorns than Père David’s rock squirrels, they also create different dispersal distance for the acorns of three species, which enhance three species’ niche separation. Our results therefore revealed that differentiations in acorn properties have derived distinct trade-offs in secondary seed dispersal strategies, likely facilitating the coexistence of the three examined species.

In Vitro Antibacterial Activity and in Silico Analysis of the Bioactivity of Major Compounds Obtained from the Essential Oil of Virola surinamensis Warb (Myristicaceae)

Essential oils are well known for their antimicrobial activity and they are used as an effective food preservative. Virola is one of the five genera of Myristicaceae and this genus is native to the American continent, especially in neotropical regions. The largest number of species of this genus is found in the Amazon region and the most important species include Virola surinamensis Warb. and Virola sebifera Aubl. In the present study, we describe the chemical composition of the essential oil of the V. surinamensis obtained at two different periods of the day in two seasons (rainy and dry), as well as their antimicrobial activity against pathogenic bacterial strains of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. In addition, we investigated, using in silico tools, the antimicrobial activity of the major chemical compounds present in the essential oil of V. surinamensis. The samples collected at different seasons and times showed a similar chemical profile, characterized by the major constituents α-pinene (>33%) and β-pinene (>13%). The essential oil of V. surinamensis showed an interesting antibacterial activity, exhibiting low inhibitory concentrations against the tested bacterial species. The computational investigation indicated that limonene, myrcene, and β-pinene could be related to the antibacterial activity against the tested pathogenic bacterial strains. Our results shed light on the possible constituents of essential oil that could be related to its activity against bacterial species and might be useful for further experimental tests that aim to discover new potential antibacterial agents for food preservation.

Occurrence of organic pollutants in the River Itchen and River Test—two chalk streams in Southern England, UK

The River Itchen and River Test, two chalk streams in Southern England, are sites of special scientific interest. These ecosystems face a number of environmental pressures from anthropogenic inputs of organic pollutants. Hence, we investigated the occurrence of these chemicals within the two catchments. Spot water samples (1 L) were collected at nineteen sites along the catchment on two occasions (March and June 2019). Samples were extracted (HLB-L sorbent disks) and analysed using high-resolution liquid chromatography-quadrupole-time-of-flight mass spectrometry and gas chromatography-mass spectrometry. Compounds were identified against commercially available databases. Using this approach, we found 115 pharmaceutical and personal care products, 81 plant protection products and 35 industrial chemicals. This complex mixture of pollutants covered a range of physico-chemical properties and included priority substances in the EU Water Framework Directive or currently on the third Watch List. Both rivers had similar chemical profiles for both months. Herbicides and fungicides were dominant in the spring, whereas insecticides occurred more frequently in the summer. Point discharges from wastewater treatment plants were the main source of pharmaceutical and personal care products. Agricultural activities were the main contributor to the presence of plant protection products. The impact of these organic chemicals on the ecology, particularly on macroinvertebrate biodiversity, is unknown and warrants further investigation. Our suspect screening approach could guide future toxicological investigations to assess the environmental impacts of these diverse chemicals.