Multidrug resistance is a rising concern for global public health. Antibiotic and antifungal resistance infections demand new antimicrobial approaches. This is the first time Silver Nanoparticles (Ag NPs) were biosynthesized using Eichhornia crassipes leaf extract as a natural reducing and stabilizing agent in Bangladesh. The Ultraviolet-Visible (UV-vis) spectra observed at 468nm and 454nm, with a maximum absorbance of 1.516 for the 1:2 ratio (60min) and 0.546 for the 1:2 ratio (30min), respectively. Fourier Transform Infrared (FTIR) spectra indicated that functional groups, including 3333.11cm-1 (51.06% T) for carboxyl, 2191.23cm-1 (97.27% T) for alkyne, 1632.58cm-1 74.89% T) for carbonyl, and 627.08cm-1 (91.6% T) for chlorinated groups in the leaf extract, probably contribute to the reduction of metallic ions and the formation of nanoparticles. The antibacterial effectiveness of the synthesized Ag NPs was evaluated against antibiotic-resistant bacterial strains obtained from patients with Urinary Tract Infections (UTIs) at Kushtia Sadar Hospital, as well as against fungal infections. The disk diffusion technique is used to evaluate the antibacterial and antifungal activity, as well as the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) tests. Notably, the synthesized Ag NPs exhibited significant antimicrobial activity against MEBTN 4(EF) and MEBTN 6(EF), with inhibition zone diameters ranging from 6 ± 0.1 to 13 ± 0.1mm. The MIC and MBC values against MEBTN 6(EF) were 15µg/mL and 60µg/mL, respectively, indicating strong bactericidal activity. Antifungal assays revealed a MIC value of 18µg/mL. The findings revealed substantial antimicrobial efficacy of Ag NPs against Multidrug-Resistant (MDR) UTI pathogens and pathogenic fungi, underscoring their broad-spectrum effectiveness in a new era of treatment. Future research should prioritize comprehensive in vivo toxicity assessments, elucidation of the underlying mechanisms, investigation of anti-cancer properties, including ROS-mediated effects, and optimization of large-scale production processes.

Anther culture is an effective biotechnological approach to accelerate the production of homozygous lines in tobacco breeding. This study evaluated the interaction between culture media (Murashige and Skoog/MS and Chu’s N6) and silver nanoparticle (AgNP) concentrations on in vitro anther regeneration of tobacco (Nicotiana tabacum L.). The experiment was arranged in a completely randomized design with ten treatment combinations consisting of two basal media and five AgNP concentrations (0, 2.5, 5, 7.5, and 10 ppm), each replicated three times. Quantitative parameters included callus formation, shoot regeneration, rooting response, regeneration percentage, and contamination rate. MS medium supplemented with 2.5 ppm AgNPs (K2) produced the optimal response, showing 100% callus formation and the highest shoot regeneration percentage (89%). Root formation was observed only in treatments K2 and K4. In contrast, higher AgNP concentrations (≥7.5 ppm), particularly in Chu’s N6 medium, significantly reduced regeneration percentages (ANOVA, p<0.05). The promotive effect of low AgNP concentration is associated with suppression of ethylene activity, antimicrobial action, and regulation of reactive oxygen species (ROS), which collectively enhance cell division and organogenesis. These findings indicate that MS medium supplemented with 2.5 ppm AgNPs is optimal for tobacco anther regeneration and suitable for doubled-haploid production.

A novel functionalized cellulose paper for wastewater treatment: Exploring its multifunctional capabilities through TA-APTES-mediated surface engineering.

Comparative toxicity of green and chemically synthesized silver nanoparticles in developing zebrafish: A step toward sustainable nanotechnology.

Silver nanoparticles treatment for Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus): In vivo and in vitro study.

Antagonistic effect of polystyrene nanoplastics and sliver nanoparticles on Chlorella pyrenoidosa.

Synergistic antibacterial activity and mechanism of surfactin and Ag NPs against Pseudomonas fluorescens.

Toxic effects and mechanism of silver nanoparticles with different particle sizes and surface coatings in lung epithelial cells.

Biobased chitosan films incorporating rosemary-mediated silver nanoparticles for enhanced antimicrobial activity and catalytic applications.

Biochemical clocks in fingerprints energy transfer between free tryptophan and NADH for potential estimation of postmortem interval, age, and sex.

Chitosan coating functionalized with silver nanoparticles synthesized from avocado leaves and cinnamon oil/β-cyclodextrin: An eco-friendly approach to avocado preservation.

Combined effects of nanomaterials and climate change on aquatic ecosystems: Toxicity, interactions, and regulatory challenges.

Coupling silver dual atoms and nanoparticles on carbon support for efficient electroreduction N2 to ammonia

Investigations on sweet flag (Acorus calamus L.) based silver and zinc nanoparticles against Sitophilus oryzae L. in wheat

Green synthesis of silver nanoparticles using Pisonia alba and toxicity assessment against Spodoptera litura across developmental stages

Green synthesis of chitosan-functionalized silver nanoparticles using non-thermal plasma as biocompatible antimicrobials against multidrug-resistant pathogens

Ratiometric electrochemical sensor based on metal-organic framework and nanosilver as response signals for cTnI detection.

Green synthesis and antibacterial activity of silver nanoparticles using Pyracantha coccinea extract

Harnessing Nature's Chemistry: Eco-friendly fabrication of copper, iron and silver nanoparticles for an environmental toxin biosensor

Emission based sensing of N-acetyl cysteine via silver equipped anthraquinone derivatives.