Silica Gel Fractionation Research Articles

Characterization of Mannide Monooleate

Two lots of a specially treated mannide monooleate emulsifier were fractionated by column chromatography and characterized by TLC and GC; individual components were identified by mass spectrometry. Silica gel fractionation indicated the presence of three major classes of components: diesters, monoesters, and polymeric carbohydrate material. The diester contents of Lots 114B and 115B were 36.3 and 51.8 wt. % while the monoester content was 52.2 and 31.4 wt. %, respectively. The carbohydrate polymer content amounted to 11.2 and 15.8 wt. %, respectively. The mannide monooleate (as the chemical entity) content within Lots 114B and 115B was 33.6 and 20 wt. %, respectively, while the mannide dioleate content varied from 21.9 wt. % (114B) to 29.2 wt. % (115B). Six cyclic fatty acids were observed in these two lots as well as in two toxic lots identified as 8B and 3589. The UV absorptions at 267, 278, 298, and 315 nm. were approximately fivefold greater for Lot 8B than Lot 114B and approximately 20‐fold greater for Lot 3589. Nonconjugated trans‐olefins were present in Lots 114B and 115B to the extent of 5.9 and 6.0 wt. %, respectively, while the conjugated tratts‐trans‐diene content amounted to about 1.6 wt. % for both specially treated lots. In both lots, 14 components were found to comprise the monoester class, while 13 components comprised the diester class. The carbohydrate material was not characterized.