Significant Pairwise Differences Research Articles

Variations in Facial Anthropometric Measurements among Major Ethnic Groups in Nigeria: A 3-Dimentional Stereophotogrammetry Analysis

Objectives: The aim of the study is to highlight variations in facial anthropometric measurements among the major ethnic groups in Nigeria using 3D photogrammetry. Materials and Methods: This study was conducted in Lagos, Nigeria. Acquisition of 3D facial photographs was done using a Vectra H1 portable 3D photogrammetry imaging system. Descriptive analysis of collected data was done and a test of associations performed using independent samples t-test. The level of significance was set at <.05. Results: The total number of participants in this study was 503. The study population was made up of 302 (60%) males and 201 (40%) females. Mean upper facial height among Yoruba, Igbo, Hausa, and other ethnic groups were 68.93 ± 5.85, 70.18 ± 5.99, 63.79 ± 4.80, and 69.29 ± 5.95 mm respectively, while mean interpupillary distances were 66.99 ± 3.70, 67.34 ± 3.56, 66.73 ± 3.80, and 67.09 ± 3.72 mm respectively. A Tukey posthoc test revealed significant pairwise differences for upper facial height between the Yoruba and Hausa population with a mean difference of 5.15 mm ( P < .001, 95% CI 2.94-7.35) ( P < .001, 95% CI 2.94-7.35), and Hausa and Igbo population with a mean difference of 6.39 mm ( P < .001, 95% CI 3.75-9.03). Multivariate regression analysis confirmed the presence of significant inter-ethnic differences between Hausa ethnic group and the Yoruba ethnic group for midfacial height ( P < .001), upper lip height ( P = .004), lower lip height ( P = .003), total face height ( P = .010), and orbital fissure width ( P < .001). Conclusion: The result of this study shows that distinct difference does exist in the facial anthropological measurements between the ethnicities included in this study especially in vertical facial measurements.

Cerebral Metabolic Stress Is Lower in Patients with Sickle Cell Anemia on Hydroxyurea Therapy

Hydroxyurea (HU) is the only medication available with neuroprotective potential in sickle cell anemia (SCA), yet the physiological mechanisms of this effect remain unclear. We utilized MRI to measure cerebral blood flow (CBF) and oxygen extraction fraction (OEF) to test our hypothesis that HU therapy relieves ongoing cerebral metabolic stress by decreasing OEF in patients with SCA. Brain MRI measured CBF (pseudo-continuous arterial spin labeling), OEF (asymmetric spin echo sequence that measures voxel-wise tissue deoxyhemoglobin) and vasculopathy (magnetic resonance angiography) in prospectively enrolled participants with SCA. FMRIB9s Linear Image Registration Tool individually co-registered CBF and OEF maps to corresponding T1 images, and FMRIB9s Automated Segmentation Tool segmented gray and white matter (GM and WM). For analysis, participants were cohorted by SCA therapy they were receiving at the time of the MRI. Group comparisons between SCA without HU, SCA with HU, and SCA pre-transfusion (pre-tx; MRI up to 24 hours before chronic tx therapy) were made with Kruskal-Wallis or chi-squared test, followed by pairwise comparisons with Mann-Whitney U test. Bivariate correlations were described with Spearman9s rho. Significance was specified as p-value Seventy-nine participants with SCA (70 HbSS, 9 HbSβ 0 thalassemia) underwent MRI. There was no significant difference in age (p = 0.965) or sex (p = 0.471) among treatment cohorts. Hemoglobin (Hb) was significantly different between cohorts (p = 0.001). While the SCA without HU cohort had a significantly lower Hb than both SCA with HU (p = 0.010) and SCA pre-tx (p There was no difference in GM (p = 0.680) or WM (p = 0.736) CBF among the 3 cohorts, even after controlling for age with multiple regression. However, there was a significant difference in GM OEF among cohorts (p To investigate potential mechanisms of metabolic stress reduction, we evaluated the relationship between OEF, total Hb, % Hb A, % Hb F and % Hb S. OEF and Hb were strongly correlated in both GM and WM (GM: spearman9s rho = -0.857, p 2 = 0.737, p = 0.003), and for WM OEF (total Hb: β = -0.041, p 2 = 0.719, p We report the first investigation into the effects of HU on cerebral oxygen metabolism in SCA. Our data suggest that HU mitigates ongoing cerebral metabolic stress by improving cerebral oxygen delivery, allowing reduction in OEF without altering CBF; however, the degree of OEF correction is not as strong as chronic tx therapy. Longitudinal evaluation of these cohorts is needed to determine if the improvement in OEF by HU is sufficient for prevention of stroke and cognitive dysfunction in patients with SCA. Disclosures Fields: Proclara Biosciences: Equity Ownership. Hulbert: Pfizer: Other: Spouse employment at Pfizer.

What Happened Pre- and during COVID-19 in South Korea? Comparing Physical Activity, Sleep Time, and Body Weight Status

The purpose of the current study is to investigate the changes in physical activity (PA), sleep time (ST), and body weight (BW) Pre- and during COVID-19 in South Korea, and specifically, PA data were obtained during COVID-19 at three-time points based on the multilevel social distancing policies. All data were surveyed by questionnaires online and offline, and participants were required to fill in the monthly average of daily step counts were recorded an application on participants’ smartphone devices from Pre-COVID-19 (2019 year) and during COVID-19 (2020 year). Participants were 834 adults (males: 54.4%, female: 45.6%) and all statistical analyses were summarized by SPSS 25.0 program. The monthly average of daily step counts was 6747.09 during Pre-COVID-19, but the PA during COVID-19 was 5812.11 daily step counts per month. Also, there were significant pairwise differences between average PA Pre-COVID-19 and each level of social distancing (p < 0.001). After COVID-19, the participants who slept less than 7 h decreased by 3.6%, while those who slept more than 9 h increased by that much. As a result of BW, 269 participants responded their BW changed during COVID-19, and 199 of them reported they gained BW during COVID-19 (74.0%). Although self-reported questionnaires may have led to an under-or over-estimation of ST and BW, the present study found that the environment in which the COVID-19 is prevalent had adverse relationships on PA, ST, and BW. Therefore, it is important to identify strategies to motivate individuals for remaining physically active and getting adequate sleep while maintaining social distancing due to the presence of the COVID-19 global pandemic.

POS0410 BIOMARKERS REFLECTING DISTURBED GUT BARRIER DIFFER IN PATIENTS WITH SPONDYLOARTHRITIS, CROHN’S DISEASE AND ACUTE ANTERIOR UVEITIS

Background:Spondyloarthritides (SpA) are characterized by frequent extra-musculoskeletal manifestations (EMM) among them acute anterior uveitis (AAU) and Crohn’s disease (CD). Vice versa, about 50% of AAU and 20% of CD patients have concomitant SpA. SpA patients show gut dysbiosis together with frequent subclinical gut inflammation. Biomarkers reflecting disturbed gut barrier (intestinal-fatty acid binding protein (iFABP), lipopolysaccharide binding protein (LBP) and zonulin) were previously found to be elevated in patients with radiographic axial SpA (r-axSpA) (1).Objectives:To evaluate whether biomarkers reflecting leaky gut are altered in patients with AAU, CD and SpA compared to healthy controls and whether they differ between patients with EMM with and without concomitant SpA.Methods:A total of 100 patients from the German Spondyloarthritis Inception Cohort (GESPIC) were included – among them 20 patients with r-axSpA without EMM, 40 patients with CD and 40 patients with non-infectious AAU – out of which 19 and 20 patients, respectively, had concomitant SpA (11/8 and 20/0 axial/peripheral SpA, respectively). The GESPIC patients were compared to 20 age- and sex-matched healthy donors (HD). The following five serum biomarkers were analyzed with ELISA: calprotectin, iFABP, LBP, soluble CD14 (sCD14) and zonulin.Results:Patient characteristics are shown in Table 1. Serum levels of calprotectin, LBP, sCD14 and zonulin differed significantly between patients with r-axSpA, AAU and CD with and without concomitant SpA and HD (Figure 1). When comparing patients with EMM with and without underlying SpA, calprotectin serum levels were significantly elevated in CD patients with SpA (8.6µg/ml (SD 5.5µg/ml)) compared to CD patients without SpA (5.7µg/ml (SD 4.1µg/ml); Mann-Whitney U Test, p=0.031). Serum levels of the analyzed biomarkers did not differ between AAU patients with and without axSpA. Spearman rank correlation revealed a significant association between CRP and calprotectin (correlation coefficient r=0.230; p=0.012), LBP (r=0.596; p&lt;0.0001), sCD14 (r=0.428; p&lt;0.0001) and zonulin (r=0.221; p=0.016), respectively. Furthermore, LBP and zonulin serum levels correlated positively (r=0.208; p=0.023); as well as LBP and sCD14 levels (r=0.418; p&lt;0.0001).Table 1.Patient characteristics. Mean values (standard deviation) or absolute numbers are shown.CD + SpACDr-axSpAAAU + axSpAAAUHDN192120202020Age39.1 (11.3)38.7 (14.4)38.4 (10.3)39.6 (12.0)39.2 (12.5)38.6 (12.9)Male (%)9 (47%)9 (43%)9 (45%)9 (45%)9 (45%)9 (45%)HLA-B27 positive (%)5 (26%)3 (14%)17 (85%)17 (85%)13 (65%)2 (10%)CRP in mg/l14.3 (25.6)18.0 (41.8)9.1 (11.3)6.7 (9.9)2.3 (3.4)0.6 (0.7)ASDAS2.8 (1.1)3.2 (0.6)2.2 (1.0)BASDAI4.1 (2.2)5.4 (1.2)3.2 (2.4)Figure 1.Biomarkers reflecting disturbed gut barrier show distinct signatures in patients with acute anterior uveitis, Crohn’s disease and axial Spondyloarthritis. Kruskal Wallis Test; p values shown. Dunn-Bonferroni Post-Hoc analyses, significant pairwise differences are marked; * p&lt;0.05; **p&lt;0.01; ***p&lt;0.0001Conclusion:We found substantial differences in biomarkers reflecting disturbed gut barrier between with SpA, CD, AAU and healthy controls. The presence of SpA was associated with higher calprotectin serum levels in CD as compared to CD without SpA.

A110 PATIENT-GUIDED COMPLICATION TRACKING SYSTEM (PACTS): BUILDING ALLIANCES WITH PATIENTS FOR THE CONTINUED IMPROVEMENT OF POST-PROCEDURAL OUTCOMES.

Abstract Background In Healthcare, interventions using Short Message Service (SMS) are growing as more patients have mobile phones. To date, studies have investigated using SMS to remind patients of upcoming appointments and provide preventative medical care. Although SMS interventions exist, little is known about their potential as a post-procedural follow-up tool. SMS follow-up systems present a unique opportunity for clinics to provide support to patients having unplanned post-procedural events. Moreover, the identification of these cases promotes the adoption of preventative measures. Before SMS follow-up programs can be integrated in clinics, proof-of-concept research needs to be conducted to assess the feasibility of this intervention. Aims This study aims to determine intervention design elements to maximize the response rate of a novel follow-up program implemented at St. Paul’s Hospital in Vancouver, BC. Methods An iterative prospective study was conducted to assess the effects of various design features on the response rate of an SMS follow-up system. Outpatients having a colonoscopy and/or gastroscopy at St. Paul’s Hospital between 11/19-03/20 were considered for inclusion in this pilot. Patients were asked to participate if they understood Grade 10-level English and had a mobile phone. For this pilot, a PAtient-guided Complication Tracking System (PACTS) was designed to send SMS to patients one week post-procedure. During each program round, adjustments were made to PACTS with the goal of increasing the response rate. The design changes made to the pilot were cumulative. One-way ANOVA and Tukey’s Honestly Significant Difference tests were completed to assess response rate differences between rounds. Results A total of 1829 patients met the inclusion criteria and consented to participate in the pilot. The overall median response rate was 93%. ANOVA test revealed a statistically significant difference in response rates between rounds, F(6, 196) = 3.369, p = 0.0035. Only the mean response rates between Rounds 1 and 7 yielded a significant pairwise difference (p &amp;lt; 0.001). Conclusions The PACTS pilot demonstrates that high response rates are achievable by SMS follow-up systems. This study identified several design elements to optimize SMS intervention response rates. These features included: sending a 1st SMS that explains the program’s purpose, sending a 2nd SMS to participants that did not respond to the first, and providing pilot information to patients upon admission and discharge. Future research on SMS follow-up systems should explore designing a program that can be integrated in clinics with minimal staff involvement. Funding Agencies None

The Effects of Modified-Implement Warm-Ups on Cricket Pace-Bowling Skill.

This study investigated the acute warm-up effects of modified-implement bowling on bowling speed, accuracy, perceived rhythm and perceived sensation with a regular ball. A total of 13 male amateur pace bowlers completed 3 sessions in a randomized, counterbalanced order. Each session comprised a warm-up of 21 progressive-effort deliveries with either a regular (156g), 10% heavier (171.6g), or 10% lighter (140.4g) cricket ball followed by a 4-over pace-bowling assessment with a regular ball. Bowling speed was assessed with a radar gun, while accuracy was calculated via the radial error. Subjects rated their perceived exertion (0%-100%), rhythm (1-5 Likert scale), and sensation (1-5 Likert scale) after each delivery. The linear mixed models revealed a significant effect for warm-up condition on perceived delivery sensation (F2,916.404 = 24.137, P < .001), with a significant pairwise difference between the regular- and heavier-ball warm-up conditions of 0.20 ± 0.07 points (estimated marginal mean ± 95% confidence interval, P < .001). There were no statistically significant effects for warm-up condition on bowling speed, accuracy, and perceived delivery rhythm. These findings indicate that although the regular ball felt lighter to bowl with after using the heavier ball, there were no overall potentiating or detrimental effects of using this particular modified-implement warm-up on bowling speed, accuracy, and perceived rhythm in amateur pace bowlers. Future research is encouraged to trial other protocols for eliciting potentiation to ultimately enhance bowling speed in training or in shorter match formats (eg, Twenty20).

Effect of varying levels of expertise on the reliability and reproducibility of the digital waxing of single crowns: A preliminary in vitro study

Statement of problemThe digital waxing of single crowns can be affected by the quality of intraoral scans and use of computer-aided design (CAD) software programs. However, clinical outcomes of the resulting crowns are also affected by computer-aided manufacturing (CAM) methodologies. Studies on the effect of different levels of expertise on digital waxing are lacking. PurposeThe purpose of this in vitro study was to assess the impact of different levels of expertise on the reliability and reproducibility of margin outlining during digital waxing. Material and methodsThirty analogs of implant stock abutments (Ø4.8×4 mm) were embedded into resin blocks. To simulate different clinical situations, abutments were divided into 3 groups: 10 abutments (group GOS) received artificial gingiva and were scanned with an open system intraoral scanner, while 10 abutments with (group GIS) and 10 abutments without artificial gingiva (group IS) were scanned with an intraoral scanner within an integrated CAD-CAM system. All resulting standard tessellation language (STL) files were used by 2 different observers (an experienced CAD professional and a clinician with basic CAD knowledge) to digitally design a left mandibular central incisor in the same software program. All resulting digital crown designs were exported to STL files to assess crown margin accuracy at the coupling interface by superimposition with the control STL file of the scan body designed for the same abutment by the manufacturer. For this purpose, a CAD software program was used to automatically calculate median, minimum, and maximum deviations of margins in millimeters. Statistically significant pairwise differences among groups and between observers were assessed with the Wilcoxon signed-rank test (α=.05). ResultsFor the CAD professional, median deviations between designed crown STL files and the control STL of the scan body were 0.08 mm (range: 0.04 to 0.15) for group GOS; 0.10 mm (range: 0.06 to 0.18) for group GIS; and 0.05 mm (range: 0.03 to 0.08) for group IS. For the clinician, median deviations were 0.08 mm (range: 0.04 to 0.12) for group GOS; 0.11 mm (range: 0.07 to 0.17) for group GIS; and 0.05 mm (range: 0.04 to 0.11) for group IS. There were no significant differences between observers (P>.05). However, statistically significant differences were found between group IS and the other 2 groups (P=.001) but not between groups GOS and GIS (P>.05). ConclusionsThe present findings suggest that a digital wax pattern made with a dental CAD software program is not affected by varying levels of expertise but might be affected by subgingival margins.

Mitochondrial Haplotypes suggest Genetic Component for Habitat Preference in Blue Crabs

Atlantic blue crabs (Callinectes sapidus) are ecologically and commercially fundamental. Life stages are punctuated with migration. Adults and juveniles live in estuaries and sounds. Larval stages develop in the coastal ocean. Juvenile and adult crabs occupy habitats from high salinities to fresh water. We determined whether maturing juvenile and adult blue crab habitat use is reflected in mitochondrial cytochrome oxidase 1 haplotypes. High salinity crabs had lower haplotype diversity (0.7260 ± .03900) compared to spawning crabs (0.9841 ± .00021) and low salinity crabs (0.94154 ± .00118). Significant pairwise differences in haplotypes were found between high salinity and spawning crabs (Nm = 0.26018, p &lt; 0.001), and between high salinity and low salinity crabs (Nm = 0.19482, p &lt; 0.001) indicating a lack of gene flow. Crabs from high salinity had highly significant genetic differentiation compared to spawning crabs (Fst = 0.11830, p &lt; 0.001) and low salinity crabs (Fst = 0.09689, p &lt; 0.001). Results support the hypothesis that genetics influence habitat selection. Crab larvae mix in the coastal ocean but occupy specific habitats upon return to sounds and estuaries. These findings have implications for the management of fisheries.

Optimal 68Ga-PSMA and 18F-PSMA PET window levelling for gross tumour volume delineation in primary prostate cancer.

This study proposes optimal tracer-specific threshold-based window levels for PSMA PET-based intraprostatic gross tumour volume (GTV) contouring to reduce interobserver delineation variability. Nine 68Ga-PSMA-11 and nine 18F-PSMA-1007 PET scans including GTV delineations of four expert teams (GTVmanual) and a majority-voted GTV (GTVmajority) were assessed with respect to a registered histopathological GTV (GTVhisto) as the gold standard reference. The standard uptake values (SUVs) per voxel were converted to a percentage (SUV%) relative to the SUVmax. The statistically optimised SUV% threshold (SOST) was defined as those that maximises accuracy for threshold-based contouring. A leave-one-out cross-validation receiver operating characteristic (ROC) curve analysis was performed to determine the SOST for each tracer. The SOST analysis was performed twice, first using the GTVhisto contour as training structure (GTVSOST-H) and second using the GTVmajority contour as training structure (GTVSOST-MA) to correct for any limited misregistration. The accuracy of both GTVSOST-H and GTVSOST-MA was calculated relative to GTVhisto in the 'leave-one-out' patient of each fold and compared with the accuracy of GTVmanual. ROC curve analysis for 68Ga-PSMA-11 PET revealed a median threshold of 25 SUV% (range, 22-27 SUV%) and 41 SUV% (40-43 SUV%) for GTVSOST-H and GTVSOST-MA, respectively. For 18F-PSMA-1007 PET, a median threshold of 42 SUV% (39-45 SUV%) for GTVSOST-H and 44 SUV% (42-45 SUV%) for GTVSOST-MA was found. A significant pairwise difference was observed when comparing the accuracy of the GTVSOST-H contours with the median accuracy of the GTVmanual contours (median, - 2.5%; IQR, - 26.5-0.2%; p = 0.020), whereas no significant pairwise difference was found for the GTVSOST-MA contours (median, - 0.3%; IQR, - 4.4-0.6%; p = 0.199). Threshold-based contouring using GTVmajority-trained SOSTs achieves an accuracy comparable with manual contours in delineating GTVhisto. The median SOSTs of 41 SUV% for 68Ga-PSMA-11 PET and 44 SUV% for 18F-PSMA-1007 PET form a base for tracer-specific window levelling. Clinicaltrials.gov ; NCT03327675; 31-10-2017.

Optimal multiparametric set-up modelled for best survival outcomes in palliative treatment of liver malignancies: unsupervised machine learning and 3\xa0PM recommendations

Over the last decade, a rapid rise in deaths due to liver disease has been observed especially amongst young people. Nowadays liver disease accounts for approximately 2 million deaths per year worldwide: 1 million due to complications of cirrhosis and 1 million due to viral hepatitis and hepatocellular carcinoma. Besides primary liver malignancies, almost all solid tumours are capable to spread metastases to the liver, in particular, gastrointestinal cancers, breast and genitourinary cancers, lung cancer, melanomas and sarcomas. A big portion of liver malignancies undergo palliative care. To this end, the paradigm of the palliative care in the liver cancer management is evolving from “just end of the life” care to careful evaluation of all aspects relevant for the survivorship. In the presented study, an evidence-based approach has been taken to target molecular pathways and subcellular components for modelling most optimal conditions with the longest survival rates for patients diagnosed with advanced liver malignancies who underwent palliative treatments. We developed an unsupervised machine learning (UML) approach to robustly identify patient subgroups based on estimated survival curves for each individual patient and each individual potential biomarker. UML using consensus hierarchical clustering of biomarker derived risk profiles resulted into 3 stable patient subgroups. There were no significant differences in age, gender, therapy, diagnosis or comorbidities across clusters. Survival times across clusters differed significantly. Furthermore, several of the biomarkers demonstrated highly significant pairwise differences between clusters after correction for multiple testing, namely, “comet assay” patterns of classes I, III, IV and expression rates of calgranulin A (S100), SOD2 and profilin—all measured ex vivo in circulating leucocytes. Considering worst, intermediate and best survival curves with regard to identified clusters and corresponding patterns of parameters measured, clear differences were found for “comet assay” and S100 expression patterns. In conclusion, multi-faceted cancer control within the palliative care of liver malignancies is crucial for improved disease outcomes including individualised patient profiling, predictive models and implementation of corresponding cost-effective risks mitigating measures detailed in the paper. The “proof-of-principle” model is presented.

A one-way ANOVA test for functional data with graphical interpretation

A new functional ANOVA test, with a graphical interpretation of the result, is presented. The test is an extension of the global envelope test introduced by Myllymaki et al. (2017, Global envelope tests for spatial processes, J. R. Statist. Soc. B 79, 381--404, doi: 10.1111/rssb.12172). The graphical interpretation is realized by a global envelope which is drawn jointly for all samples of functions. If a mean function computed from the empirical data is out of the given envelope, the null hypothesis is rejected with the predetermined significance level $\alpha$. The advantages of the proposed one-way functional ANOVA are that it identifies the domains of the functions which are responsible for the potential rejection. We introduce two versions of this test: the first gives a graphical interpretation of the test results in the original space of the functions and the second immediately offers a post-hoc test by identifying the significant pair-wise differences between groups. The proposed tests rely on discretization of the functions, therefore the tests are also applicable in the multidimensional ANOVA problem. In the empirical part of the article, we demonstrate the use of the method by analyzing fiscal decentralization in European countries. The aim of the empirical analysis is to capture differences between the levels of government expenditure decentralization ratio among different groups of European countries. The idea behind, based on the existing literature, is straightforward: countries with a longer European integration history are supposed to decentralize more of their government expenditure. We use the government expenditure centralization ratios of 29 European Union and EFTA countries in period from 1995 to 2016 sorted into three groups according to the presumed level of European economic and political integration.

Titration of supplemental Bacillus subtilis subsp. subtilis American Type Culture Collection PTA-125135 to broiler chickens fed diets of 2 different metabolizable energy concentrations

Bacillus subtilis subsp. subtilis American Type Culture Collection deposit number PTA-125135 has recently been studied by our laboratory as a potential probiotic strain for avian species. The objective of the present study was to evaluate growth performance and feed efficiency in broiler chickens in response to a dose titration of the Bacillus strain in feed. In addition to a nonsupplemented control, Bacillus spores were supplemented into broiler chicken diets at 4 levels, which were 8.1 × 104, 1.6 × 105, 2.4 × 105, and 3.2 × 105 CFU per g of feed. The titration was applied to two different dietary regimes of standard or low metabolizable energy (ME), which differed in ME by 22, 56, and 110 kcal/kg in starter, grower, and finisher dietary phases, respectively. All diets contained 249 g per metric ton of a previously patented synbiotic feed additive. Performance data were collected at day 14, 26, and 40 of age, and the effects of Bacillus and ME treatments were evaluated by factorial ANOVA. Treatment group means were further examined for significant (P < 0.05) pairwise differences among treatments and for significant (P < 0.05) linear and quadratic effects. At day 14 of age, significant linear effects for decreased feed conversion ratio (FCR) with higher CFU of Bacillus supplementation were observed within the standard ME diet. At day 26, a linear trend was observed for increased mortality with increased dose within the standard ME diet only. Bacillus supplementation at day 26 also significantly affected FCR and mortality-adjusted FCR, where supplementation with 3.2 × 105 CFU per g feed produced lower FCR and mortality-adjusted FCR than supplementation with 1.6 × 105 CFU per g feed. We conclude from linear effects related to feed efficiency observed at day 14 and from the significant separation of Bacillus treatment means within the titrated range of supplementation at day 26 that further evaluation for effects on performance should be made of doses at 2.4 × 105, 3.2 × 105, and greater CFU per g in feed.

Pilot Study of the Juvenile Dermatomyositis Consensus Treatment Plans: A CARRA Registry Study.

To determine the feasibility of comparing the Childhood Arthritis and Rheumatology ResearchAlliance (CARRA) consensus treatment plans (CTP) in treating moderate new-onset juvenile dermatomyositis (JDM) using the CARRA registry, and to establish appropriate analytic methods to control for confounding by indication and missing data. A pilot cohort of 39 patients with JDM from the CARRA registry was studied. Patients were assigned by the treating physician, considering patient/family preferences, to 1 of 3 CTP: methotrexate (MTX) and prednisone (MP); intravenous (IV) methylprednisolone, MTX, and prednisone (MMP); or IV methylprednisolone, MTX, prednisone, and IV immunoglobulin (MMPI). The primary outcome was the proportion of patients achieving moderate improvement at 6 months under each CTP. Statistical methods including multiple imputation and inverse probability of treatment weighting were used to handle missing data and confounding by indication. Patients received MP (n = 13), MMP (n = 18) and MMPI (n = 8). Patients in all CTP had significant improvement in disease activity. Of the 36 patients who remained in our pilot study at 6 months, 16 (44%) of them successfully achieved moderate improvement at 6 months (6/13, 46% for MP; 7/15, 47% for MMP; 3/8, 38% for MMPI). After correcting for confounding, there were no statistically significant pairwise differences between the CTP (P = 0.328-0.88). We gained valuable experience and insight from our pilot study that can be used to guide the design and analysis of comparative effectiveness studies using the CARRA registry CTP approach. Our analytical methods can be adopted for future comparative effectiveness studies and applied to other rare disease observational studies.

Phenomics of elite heirlooms of peppers (Capsicum annuum L.) from the Spanish centre of diversity: Conventional and high-throughput digital tools towards varietal typification

Spain is a relevant secondary centre of diversity for Capsicum annuum peppers, especially for the bell types known as Pimiento Morrón or Pimiento de Morro. Thus, a myriad of highly regarded landraces adapted to a wide range of conditions can be found throughout the country, as a result of centuries of farmers breeding. Despite that, these materials lack of proper characterization, of paramount importance for farmers, breeders and germplasm management. In this regard, in addition to internationally accepted conventional descriptors, high-throughput digital phenotyping tools like Tomato Analyzer, a software originally developed to process scanned images of cut tomato fruits and to record a range of morphological parameters, may provide an important help towards exhaustive germplasm characterization. With this aim, 32 conventional and 35 Tomato Analyzer digital traits were used herein to characterize a large collection of C. annuum accessions from all Spanish regions, including Protected Designations of Origin and Protected Geographical Indications, with emphasis on Morrón peppers, in order to assess the diversity within Spanish elite germplasm and to test the efficiency of those methods to differentiate varietal types and closely related materials. A considerable amount of variation was found using both conventional and digital traits, even within Morrón pepper groups, reflecting the diversity of Spanish peppers in terms of plant and fruit morphology, essential for future breeding programs. Both conventional descriptors and digital parameters were able to distinguish varietal groups. However, on the whole, digital phenotyping was able to discriminate in a more accurate way. Most digital parameters were able to discriminate varietal groups into higher numbers of categories (≥4) than conventional traits (usually 2–4). In addition, the number of significant pairwise differences among varietal groups was considerably higher for digital parameters than for conventional descriptors, enabling a powerful separation, particularly relevant for closely related groups such as Morrón peppers. Likewise, as revealed by Principal Components Analysis, digital phenotyping allowed a more powerful intra-varietal separation compared to conventional descriptors. Finally, a subset of 4 conventional descriptors and 13 Tomato Analyzer traits were identified as the most discriminant to distinguish among closely related C. annuum accessions, explaining 81.81 % of total variance found by Principal Components Analysis. Fruit traits explained the highest percentage of variance for our collection.

Abstract TMP46: Fostering Eating After Stroke (FEAST) Trial: A Randomized Controlled Trial of Non-Invasive Brain Stimulation for Improving Dysphagia After Stroke

Background: Effective therapy for dysphagia recovery after stroke is currently lacking. We conducted a phase-2 RCT to investigate the effects of anodal transcranial direct current stimulation (atDCS) in reducing aspiration risk, improving diet, and analyzed its safety in the acute-subacute stroke phase. Hypothesis: Since swallowing has bi-hemispheric representation targeted atDCS to the unaffected hemisphere combined with swallowing exercises may improve dysphagia from a unilateral hemispheric infarction [UHI]. Methods: This phase-2, double-blind, RCT enrolled subjects with dysphagia [penetration and aspiration (PAS) score ≥ 4], from an acute-subacute UHI [day 2-day 6 after stroke onset] and randomized them into 3 groups: low-dose atDCS [2 mA atDCS alternating with sham twice daily], high-dose atDCS [2 mA atDCS twice daily] or sham [twice daily]. All sessions were performed with standardized swallowing exercises for 20 minutes over 5 days. Major safety outcomes were incidence of seizures, worsening of neurological and swallowing functions and mortality; primary efficacy outcome was a change in PAS scores between day 1 and 5 of intervention; main dietary outcome was a change in dietary intake at 1 month using the Functional Oral Intake scale (FOIS). Results: Of the planned 99 subjects, 42 could be enrolled in the funding period. No group differences in pre-defined safety outcomes were observed. Mean (SD) change in PAS score from baseline to final session was -0.8 (±1.6) in sham, -0.8 (±1.5) in low-dose and -0.4 (±1.2) in high dose groups. Adjusting for baseline PAS, NIHSS scores and age, means (SE) were -0.96 (0.33) in sham, -0.81 (0.36) in low-dose and -0.34 (0.35) in high dose; there were no significant pairwise differences (p&gt;0.40). Mean (SD) changes in FOIS scores from baseline to 1 month were: 2.1 (±1.7) in sham, 2.5 (±1.7) in low-dose and 2.9 (±1.2) in high dose. Adjusted means (SE) were 2.07 (0.35) in sham, 2.46 (0.38) in low dose and 3.05 (0.38) in high dose (pairwise p &gt; 0.15). Conclusion: Application of atDCS to the unaffected hemisphere is safe in the acute-subacute stroke phase but did not decrease aspiration risk in this phase-2 RCT. Higher doses of atDCS were associated with better dietary scores but group differences were not statistically significant.

Habitat associations and assemblage structure of demersal deep-sea fishes on the eastern Flemish Cap and Orphan Seamount

Advancements in remote-sampling and optical technologies have considerably improved our understanding of fish-habitat relationships and assemblage structure in the deep ocean through direct observations. The composition and complexity of seafloor habitats can strongly influence species diversity and distributions, but the relative importance of different microhabitats - both abiotic and biotic - is poorly understood. We examined differences in fish species composition and relative abundance between different physical (sediment type and boulder density) and biological (coral and sponge densities) habitat categories through in-situ observations from remotely-operated vehicle surveys of unglaciated deep-sea features off the coast of Newfoundland, Canada. Fish-habitat relationships were observed across 61 km of seafloor and spanning depths of 875–3003 m at five dive locations, with additional quantification of fish behaviour and assemblage patterns. Distinct assemblages occurred among depth zones (ANOSIM, Global R = 0.47, p = 0.001), although global tests of overall biological and physical habitats were not significant. Significant pairwise differences in assemblages were only observed in more complex physical habitats (e.g. boulder fields, and outcrops) and complex biological habitats (e.g. dense corals) compared to less complex areas of fine-grain sediment or locations with no or few corals and sponges present. Our results suggest overall structural complexity of physical and biogenic habitat features may be particularly important to some deep-sea fishes. Until further details of these relationships can be explored, conservation efforts should strive to protect a wide-range of microhabitats to preserve valuable fish habitats in the deep-ocean environment.

Relationship between Proactivity and Personal Pedagogical Knowledge in ESD among Pre-service Teachers: Sub-sampling on Social Media Usage and Subject Discipline

Abstract Personal Pedagogical Knowledge (PPK), one of the elementary factors of Pedagogical Content Knowledge (PCK), explains how teacher personality influences his/her unique way of teaching. It is an inevitable part of the Education for Sustainable Development (ESD). Pre-service teachers, who are participating in the knowledge management program for sustainable development, should possess strengthened or organized PPK. The curriculum of Teacher Education should have been reconfigured to conceptualize PPK as a lens to observe various teaching competences needed for sustainability. The personality of a teacher is considered right only when he/she organizes his/her behavior in a proactive way. It means the teacher should develop an ability to be in patience on the right path even though the situation is unfavorable. Proactivity organizes PPK that means the teacher personality is strong enough to make gradual changes in the unfavorable situation and turn it into favorable. This study aims at examining the relationship between Pro-activity and PPK among pre-service teachers based on their social media usage and subject discipline in the context of ESD. The major objectives are as follows: (1) to find out the extent of Proactivity and PPK among pre-service teachers in the context of ESD and (2) to find out the relationship between Proactivity and PPK among pre-service teachers. The study adopted a normative survey method by sampling 60 pre-service teachers from science and arts/humanities discipline. The sample is also categorized based on whether the pre-service teachers are users or not users of social media for propagating sustainability. Major findings are as follows: (1) there are significant pairwise differences in the Proactivity and PPK scores of high, average and low levels in the context of sustainability among pre-service teachers; (2) pre-service teachers using social media and those from arts/humanities discipline are exhibiting high levels of Proactivity and PPK than those who are not using social media and those from Science Education; and (3) there is a substantial relationship between Proactivity and PPK among pre-service teachers in whole and relevant sub-samples based on social media usage and subject-discipline regarding ESD.

Head injury metric response in finite element ATDs and a human body model in multidirectional loading regimes

Objective: The objective was to quantify head injury metric sensitivity of the 50th percentile male Hybrid III, THOR, and Global Human Body Models Consortium simplified occupant (GHBMC M50-OS) to changes in loading conditions in loading regimes that may be experienced by occupants of spaceflight vehicles or highly autonomous vehicles (HAVs) with nontraditional seating configurations.Methods: A Latin hypercube (LHD) design of experiments (DOE) was employed to develop boundary conditions for 455 unique acceleration profiles. Three previously validated finite element (FE) models of the Hybrid III anthropomorphic test device (ATD), THOR ATD, and GHBMC M50-OS were positioned in an upright 90°-90°-90° seat and with a 5-point belt. Acceleration pulses were applied to each of the three occupants in the ± X, +Y, and ± Z directions, with peak resultant acceleration magnitudes ranging from 5 to 20 G and times to peak ranging from 32.5 to 120.8 ms with duration 250 ms, resulting in 1,248 simulations. Head injury metrics included peak linear head acceleration, peak rotational head acceleration, head injury criteria (HIC15), and brain injury criteria (BrIC). Injury metrics were regressed against boundary condition parameters using 2nd order multiple polynomial regression, and compared between occupants using matched pairs Wilcoxon signed rank analysis.Results: Across the 416 matched-simulations that reached normal termination with all three models, HIC15 values ranged from 1.0–396.5 (Hybrid III), 1.2–327.9 (THOR), and 0.6–585.6 (GHBMC). BrIC ranged from 0.03–0.95 (Hybrid III), 0.03–1.21 (THOR), and 0.04–0.84 (GHBMC). Wilcoxon signed rank analysis demonstrated significant pairwise differences between each of the three occupant models for head injury metrics. For HIC15, the largest divergence between GHBMC and the ATDs was observed in simulations with components of combined underbody and rear impact loading. The three models performed most similarly with respect to BrIC output when loaded in a frontal direction. Both the GHBMC and the Hybrid III produced lower values of BrIC than the THOR on average, with the differences most pronounced in rear impact loading.Conclusion: In conclusion, observed differences between the occupant models’ head injury metric output were quantified. Loading direction had a large effect on metric outcome and metric comparability across models, with frontal and rear impacts with low vertical acceleration tending to be the most similar. One explanation for these differences could be the differences in neck stiffness between the models that allowed more rotation in the GHBMC and THOR. Care should be taken when using ATDs as human volunteer surrogates in these low energy events.

Inter and intra specific variation in echolocation signals among odontocete species in the Northwest Atlantic, the Temperate Pacific and Hawaii

Odontocete species use echolocation signals (clicks) to forage and navigate. The aim of this study is to explore inter- and intra-specific variation in clicks among odontocete species in the Northwest Atlantic, Temperate Pacific, and Hawaii. Clicks were examined for seven species of odontocetes—bottlenose dolphins, common dolphins, striped dolphins, rough-toothed dolphins, pilot whales, Risso’s dolphins, and Cuvier’s beaked whales. Specially developed PAMGuard tools were used to automatically measure a suite of click parameters. Seven parameters were compared within and between species; duration, 10 dB bandwidth, 3 dB bandwidth, center frequency, peak frequency, sweep rate, and number of zero crossings. Significant differences in duration, center, and peak frequency were evident between species within study areas (Dunn’s test with Benjamini-Hochberg adjustment <0.05). Geographic variation in click parameters between the three study regions was also examined. Results showed statistically significant pair-wise differences between geographic regions for almost all echolocation click parameters and species (Dunn’s test with Benjamini-Hochberg adjustment <0.05). Results suggest species-specific differences in clicks among odontocetes and indicate that geographic variation exists for multiple species. The ecological significance of these findings will be discussed along with implications for classifier development.

Analysis of the mitochondrial CYTB gene sequence in human populations of northeastern Bosnia

This study offers the first report on variation sequence of the mitochondrial cytochrome b (MTCYTB) gene in populations from Bosnia (northeastern Bosnia). This study was designed on the analysis of the genetic diversity of two populations of different cultural-anthropological and genetic origin, Roma population and native/non-Roma population. The main aim of our study was to estimate the usefulness of the CYTB sequence in the analysis of genetic categorization of different populations and intergroup diversity, as well as to provide some additional information on haplogroup-associated polymorphisms within the CYTB region in defining haplogroup status. Estimation of the genetic diversity was done using intra and intergroup genetic indices. The population-specific polymorphisms have been found in both categories of the populations. The results of the analysis of genetic differentiation show significant pairwise Fst differences between the Romani and native populations. Also, registered significant genetic differentiation is illustrated on the level of genetic variation between subpopulations of the Roma and non-Roma origin. The important result in our study is the confirmation of the significance of the triad of polymorphisms T14783C-G15043A-G15301A, indicating the influence of Asian component of the maternal gene pool on the genetic structure of the studied population of the Roma. Our data show that the haplogroup polymorphisms exist in the CYTB region and can provide useful information on the haplogroups that were defined only by the control region of the mtDNA. The results of this study indicate the region of CYTB gene can be a benefit in providing some additional information in the analysis of genetic structure of human populations and can be additionally applied in population studies.