Sida Spp Research Articles

Densitometric method for assessment of six specialized metabolites in four Sida sp. and its congener Abutilon indicum: Targeted metabolomics, greenness assessment, and chemometrics analysis

Sida is one of the most diverse genera, with about 200 species distributed in tropical and subtropical regions of the world. Among 18 species distributed in India, Sida acuta, Sida cordifolia, Sida rhombifolia, and Sida cordata are used in traditional medicines along with its possible adulterant Abutilon indicum for several therapeutic uses. The non-availability of marker-based validated methods for the identification and classification of these species leads to adulteration. Indoloquinoline and quinazoline are the major bioactive alkaloids distributed in Sida spp. First time, a simple, economical and high throughput method was developed and validated for the simultaneous determination of 20-hydroxyecdysone (1), vasicine (2), vasicinone (3), cryptolepine (4), quindolinone (5), and cryptolepinone (6) using HPTLC-UV densitometry. The method was validated to meet globally accepted ICH guidelines. The method was sensitive with LOD and LOQ ranging from 0.38–0.63 and 1.57–2.12 µg/band. The samples were spiked at 3 different concentrations, the recovery values were 93.49–98.88%. In addition, the greenness index of the HPTLC method was estimated using four different greenness assessment techniques. Targeted HPTLC analysis indicated the distribution of specialized metabolites in Sida spp. and A. indicum. However, the occurrence of cryptolepine in A. indicum was not reported in the literature, so this was further confirmed by liquid chromatographic studies of the samples from different locations. The chromatographic data was statistically evaluated by principal component analysis (PCA) and hierarchical clustering (HCA). HPTLC-based targeted metabolite quantitation explains the adulteration/substitution in Sida raw material and derived herbal preparations.

Sida Golden Mosaic Virus, an Emerging Pathogen of Snap Bean (Phaseolus vulgaris L.) in the Southeastern United States.

Sida golden mosaic virus (SiGMV) was first detected from snap bean (Phaseolus vulgaris L.) in Florida in 2006 and recently in Georgia in 2018. Since 2018, it has caused significant economic losses to snap bean growers in Georgia. This study, using a SiGMV isolate field-collected from prickly sida (Sida spinosa L.), examined the putative host range, vector-mediated transmission, and SiGMV-modulated effects on host-vector interactions. In addition, this study analyzed the phylogenetic relationships of SiGMV with other begomoviruses reported from Sida spp. Host range studies confirmed that SiGMV can infect seasonal crops and perennial weed species such as snap bean, hollyhock (Alcea rosea L.), marsh mallow (Althaea officinalis L.), okra (Abelmoschus esculentus (L.) Moench), country mallow (Sida cordifolia L.), prickly sida (S. spinosa), and tobacco (Nicotiana tabacum L.). The incidence of infection ranged from 70 to 100%. SiGMV-induced symptoms and virus accumulation varied between hosts. The vector, Bemisia tabaci Gennadius, was able to complete its life cycle on all plant species, irrespective of SiGMV infection status. However, SiGMV infection in prickly sida and country mallow positively increased the fitness of whiteflies, whereas SiGMV infection in okra negatively influenced whitefly fitness. Whiteflies efficiently back-transmitted SiGMV from infected prickly sida, hollyhock, marsh mallow, and okra to snap bean, and the incidence of infection ranged from 27 to 80%. Complete DNA-A sequence from this study shared 97% identity with SiGMV sequences reported from Florida and it was determined to be closely related with sida viruses reported from the New World. These results suggest that SiGMV, a New World begomovirus, has a broad host range that would allow its establishment in the farmscapes/landscapes of the southeastern United States and is an emerging threat to snap bean and possibly other crops.

English

In order to identify combinations of effective preemergent herbicides for weeds in sugarcane, an experiment was conducted at the Agricultural School of Concepción; Concepción, Paraguay. The experimental model was subdivided plots, with two factors: A1 without herbicide for broadleaf weeds, A2 diuron (dose 3 t ha-1), A3 atrazine (dose 4 kg ha-1), A4 sulfentrazone (dose 1.2 t ha-1) and B1 without herbicide for weeds, B2 trifluralin (dose 3 l ha-1), B3 acetochlor (dose 1 l ha-1), forming twelve treatments with three repetitions. They were applied in preemergence, where the evaluations were from 20 to 41 days after application (DDA), for: degree of control, residual effect and control of predominant weeds. The combination of diuron and acetochlor, produced high weed control (more 90% up to 41 days DDA), being superior to the other treatments. Sida spp was better controlled with diuron; whereas Mollugo verticillata was effectively controlled with most combinations; Ipomoea nil was more sensitive to sulfentrazone, while; Cenchrus echinatus was effectively controlled, with the combination of diuron plus acetochlor. It is concluded that with the combination of diuron and acetochlor, a large variety of weeds was controlled and the highest residual effect was recorded. In addition, atrazine combined with graminicides (trifluralin or acetochlor) or diuron with acetochlor, provided total control of weeds. It is recommended: for Sida spp. diuron with acetochlor or atrazine. For Mollugo verticillata, sulfentrazone alone or combined with graminicides. Ipomoea nil, sulfentrazone. Cenchrus echinatus, diuron and acetochlor combined. Key words: Saccharum officinarum, weeds, preemergence herbicides.  

The ever increasing diversity of begomoviruses infecting non‐cultivated hosts: new species from Sida spp. and Leonurus sibiricus, plus two New World alphasatellites

AbstractBegomoviruses (whitefly‐transmitted, single‐stranded DNA plant viruses) are among the most damaging pathogens causing epidemics in economically important crops worldwide. Besides cultivated plants, many weed and wild hosts act as virus reservoirs where recombination may occur, resulting in new species. The aim of this study was to further characterise the diversity of begomoviruses infecting two major weed genera, Sida and Leonurus. Total DNA was extracted from samples collected in the states of Rio Grande do Sul, Paraná and Mato Grosso do Sul during the years 2009–2011. Viral genomes were enriched by rolling circle amplification (RCA), linearised into unit length genomes using various restriction enzymes, cloned and sequenced. A total of 78 clones were obtained: 37 clones from Sida spp. plants and 41 clones from Leonurus sibiricus plants. Sequence analysis indicated the presence of six bipartite begomovirus species and two alphasatellites. In Sida spp. plants we found Sida micrantha mosaic virus (SiMMV), Euphorbia yellow mosaic virus (EuYMV), and three isolates that represent new species, for which the following names are proposed: Sida chlorotic mottle virus (SiCMoV), Sida bright yellow mosaic virus (SiBYMV) and Sida golden yellow spot virus (SiGYSV), an Old World‐like begomovirus. L. sibiricus plants had a lower diversity of begomoviruses compared to Sida spp., with only Tomato yellow spot virus (ToYSV) and EuYMV (for the first time detected infecting plants of the genus Leonurus) detected. Two satellite DNA molecules were found: Euphorbia yellow mosaic alphasatellite, for the first time detected infecting plants of the genus Sida, and a new alphasatellite associated with ToYSV in L. sibiricus. These results constitute further evidence of the high species diversity of begomoviruses in non‐cultivated hosts, particularly Sida spp.

Genetic structure of populations of the begomoviruses Tomato mottle leaf curl virus and Sida mottle Alagoas virus infecting tomato (Solanum lycopersicum) and Sida spp., respectively

Begomoviruses are among the most damaging pathogens infecting cultivated hosts worldwide, and are considered an important limiting factor in tomato fields. In the present study, foliar samples of tomato and Sida spp. were collected in Boqueirao, state of Paraiba, Brazil, in May 2014. Viral genomes were amplified, cloned and sequenced. A total of 30 DNA-A components were obtained. Pairwise comparisons indicated the presence of only two begomovirus species: Tomato mottle leaf curl virus (ToMoLCV) from tomato samples and Sida mottle Alagoas virus (SiMoAV) in Sida spp. ToMoLCV and SiMoAV subpopulations showed high genetic variability, with the ToMoLCV Rep gene being the most variable. Recombination events were detected only among ToMoLCV isolates, with recombination breakpoints occurring in the common region and in the Rep gene. Negative or purifying selection seems to be among the most important evolutionary mechanisms acting on the CP and Rep genes in both ToMoLCV and SiMoAV. Our results indicate that ToMoLCV remains as the main tomato-infecting begomovirus in northeastern Brazil, and that Sida spp. do not contribute to ToMoLCV epidemics in tomato.

The status of weed biological control in Vanuatu

Biological control of weeds in Vanuatu began in 1935, with the introduction of the tingid Teleonemia scrupulosa to control Lantana camara. To date, nine biological control agents have been intentionally introduced to control eight weed species. Seven of these agents have established on their respective hosts while an eighth, Zygogramma bicolorata, an agent for Parthenium hysterophorus has only recently been released and establishment is unlikely. The fate of a ninth agent, Heteropsylla spinulosa, released for the control of Mimosa diplotricha is unclear. Six other biological control agents, including Epiblema strenuana which was first detected in 2014 on P. hysterophorus on Efate have spread into the country unintentionally. Control of the target weeds range from inadequate to very good. By far the most successful agent has been Calligrapha pantherina which was introduced to control Sida acuta and Sida rhombifolia. The beetle was released on 14 islands and managed to spread to at least another 10 islands where it has effectively controlled both Sida spp. Control of the two water weeds, Eichhornia crassipes by Neochetina bruchi and N. eichhorniae and Pistia stratiotes by Neohydronomus affinis, has also been fairly good in most areas. Two agents, T. scrupulosa and Uroplata girardi, were released on L. camara, and four other agents have been found on the weed, but L. camara is still not under adequate control. The rust Puccinia spegazzinii was first released on Mikania micrantha in 2012 and successfully established. Anecdotal evidence suggests that it is having an impact on M. micrantha, but detailed monitoring is required to determine its overall impact. Future prospects for weed biological control in Vanuatu are positive, with the expected greater spread of recently released agents and the introduction of new agents for P. hysterophorus, L. camara, Dolichandra unguis-cati and Spathodea campanulata.

Leaf area and epicuticular wax content of Sida spp

Morpho-physiological characteristics and chemical composition are directly related to superior competitive ability of crops. This study intended to make a comparative analysis of dry matter production, leaf area and amount of epicuticular wax of three species of Sida spp: S. urens L., S. rhombifolia L. and S. spinosa L. Plants were collected at three growth stages: V1: stage described as up to 10 fully expanded leaves; V2: between 11 leaves and flowering; and R: after flowering. At stages V2 and R, the highest number of leaves was recorded for S. rhombifolia, followed by S. spinosa at V2 and S. urens at R. These results were relatively proportional to leaf area for all species. S. spinosa at the vegetative stage produced the highest values of specific leaf area (SLA), with no significant differences between species at the stage R. The amount of wax per unit of leaf area between species at the same developmental stage was significantly different only at the reproductive stage, where S. spinosa produced 23.18 and 6.23 fold more wax than S. urens and S. rhombifolia respectively. Between the growth stages of each species, there was decrease in the amount of wax with plant age and increase in leaf area (AFE), number of leaves and dry matter. The leaves of the Sida species exhibit different characteristics and this information can be used to optimize the use of herbicides in the control of these weeds.

Phytosociological Survey of Weeds in Areas of Crop-Livestock Integration

The dynamics of the weeds in the areas of crop-livestock integration is constantly changing depending on the various uses of the area. This study identified and quantified the floristic composition of weeds in areas of integration of maize for silage with Urochloa ruziziensis cv. Common at different densities of grass (0, 4 and 10 kg&middotha-1) to grazing of beef cattle. The experimental design was completely randomized. A square of side0.5 m(0.25 m2area) was launched 24 times in each study area. The survey was conducted at the grain filling stage of maize. The specie contained in each frame were identified and counted. The phytosociological survey identified 9 families of weeds in areas assessed, with 7 families in every area of integrated cultivation maize with Urochloa ruziziensis at densities of 10 kg&middotha-1 and 4 kg&middotha-1 and 8 families in the area of maize cultivated alone. The Asteraceae family was the most represented in number of species. Sida spp (Malvaceae) showed greater potential to cause damage to maize. Urochloa ruziziensis at planting density of 10 kg&middotha-1 provided greater competition with weeds in the integration with maize.

Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp

Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants. The aim of this study was to further characterize the diversity of begomoviruses infecting weeds (mostly Sida spp.) in Brazil. Total DNA was extracted from weed samples collected in Viçosa (Minas Gerais state) and in some municipalities of Alagoas state in 2009 and 2010. Viral genomes were amplified by RCA, cloned and sequenced. A total of 26 DNA-A clones were obtained. Sequence analysis indicated the presence of 10 begomoviruses. All viral isolates from Blainvillea rhomboidea belonged to the same species, Blainvillea yellow spot virus (BlYSV ), thereby suggesting that BlYSV may be the only begomovirus present in this weed species. Four isolates represent new species, for which the following names are proposed: Sida yellow blotch virus (SiYBV), Sida yellow net virus (SiYNV), Sida mottle Alagoas virus (SiMoAV) and Sida yellow mosaic Alagoas virus (SiYMAV). Recombination events were detected among the SiYBV isolates and in the SiYNV isolate. These results constitute further evidence of the high species diversity of begomoviruses in Sida spp. However, the role of this weed species as a source of begomoviruses infecting crop plants remains to be determined.

Efeito da época de semeadura nas relações de interferência entre uma comunidade infestante e a cultura do amendoim

A presente pesquisa foi realizada com o objetivo de avaliar o efeito da época de semeadura sobre os períodos de interferência das plantas daninhas na cultura do amendoim-rasteiro (Arachis hypogaea cv. IAC Runner 886). Dois experimentos foram instalados em épocas diferentes - início de novembro e de dezembro de 2005 em áreas vizinhas e provenientes de reforma de canavial, no município de Jaboticabal, SP, num solo de textura média. O delineamento experimental foi de blocos ao acaso, com os tratamentos constituídos por períodos crescentes de convivência ou de controle das plantas daninhas com as plantas de amendoim. As plantas daninhas que se destacaram em importância relativa, na primeira e segunda épocas, foram Ipomoea triloba, Digitaria nuda, Hyptis lophanta e Sida spp. A comunidade infestante e a cultura foram influenciadas pela época de semeadura, modificando suas relações de interferência. Os valores de acúmulo de massa seca da parte aérea das plantas daninhas na segunda época superaram em 23,7% os encontrados na primeira época durante todo o ciclo de desenvolvimento da cultura, refletindo, principalmente, em redução de produtividade de vagens de amendoim. Admitindo 5% de tolerância na redução da produtividade de vagens, verificou-se que o período anterior à interferência (PAI) na primeira época foi de 28 dias após a emergência (DAE), e o período total de interferência (PTPI), de 78 DAE, com redução de produtividade de 53,5%. Na segunda época, os efeitos da convivência da comunidade infestante com a cultura foram mais drásticos, pois afetaram com maior intensidade a produtividade da cultura, reduzindo-a em 86%, com PAI de 33 DAE e PTPI de 93 DAE. Quando no limpo, durante todo o ciclo da cultura, o amendoim semeado mais cedo (início de novembro) apresentou produtividade de vagens maior, superando em 23,7% o semeado um mês mais tarde.

Diagnóstico e perspectivas do manejo de plantas daninhas na cultura da pupunha no Noroeste do Estado do Paraná

A field survey accomplished for 24 areas cropped with peach palm in northwest Paraná provided enough information to show a high occurrence of weeds such as Brachiaria decumbens, Cenchrus echinatus, Panicum maximum, Emilia sonchifolia, Sida spp, and Sida micranthum. Most difficult weeds to control are perennial such as Cynodon dactylon, Cyperus spp. and S. micranthum), or those where glyphosate is less effective (Commelina benghalensis, Heteranthera limosa). A preliminar greenhouse assay, with the aim of evaluating herbicides with potential to be used in this crop demonstrataed that, among 22 herbicides evaluated, atrazine, [atrazine+simazine], azafenidin, clethodim, fluazifop, halosulfuron, nicosulfuron, sethoxydim and tebuthiuron were the most selective. In as second greenhouse assay, herbicides were applied directly to soil surrounding plants and in plants over top. Applied in pre-emergence, [diuron+MSMA], [diuron+paraquat], 2,4-D, glyphosate, MSMA and oxyfluorfen did not cause injuries in peach palm plants, being this mode considered as the most promissory.

Efeitos de períodos de convivência das plantas daninhas sobre a produtividade da cultura do amendoim das secas

Com o objetivo de estudar os efeitos de diferentes períodos de matocompetição sobre o crescimento e produtividade da cultura do amendoim cultivar Tatu-53, conduziu -se o presente experimento sobre solo Latossol Vermelho escuro fase arenosa na região de Jaboticabal, SP. Constituíram-se os tratamentos de vários períodos de convivência da comunidade infestante na cultura do amendoim, considerados à partir da emergência da cultura até 14, 28, 42, 56, 70, 84 e 117 dias (colheita). Após o período de convivência, a cultura foi mantida no limpo através de capinas na entre-linha e monda na linha de semeadura, a cada duas semanas. Houve ainda, um tratamento em que a cultura foi mantida no limpo durante todo o ciclo. O espaçamento foi de 0,60 m com densidade de 25 sementes por metro de sulco. As principais espécies de plantas daninhas que ocorreram na área experimental foram Cenchrus echinatus L., Digitaria sanguinalis (L.) Scop., Alternantera ficoidea (L.) R. Br., Emilia sonchifolia DC. e Sida spp. O período em que a competição torna-se crítica para a produtividade do amendoim inicia-se entre 42 e 56 dias do ciclo da cultura. Os parâmetros produtivos afetados pela matocompetição foram produção de vagens e grãos por hectare e por indivíduos, número médio de vagens por planta e por parcela e distribuição de grãos de diferentes tamanhos na massa produzida.

Sementes nocivas que ocorreram em amostras de sementes de azevém (Lolium multiflorum), analisadas no Rio Grande do Sul nos anos de 1978 e 1979

Foram levantadas informações sobre a qualidade da semente de azevém utilizadas no Rio Grande do Sul e produzida no próprio Estado ou em outras uni dades da Federação e mesmo em outros Países, nos anos de 1978 e 1979. Estas informações foram obtidas através de fichas e Boletins de Análise de Sementes dos Laboratórios de Análise de Sementes (LAS) do Rio Grande do Sul. Em 1978 analisou-se 2.319 t de azevém sendo 74% da semente oriunda do Rio Grande do Sul e 26% introduzida, enquanto que das 4.772 t analisadas em 1979 99,6% são do Rio Grande do Sul e 0,4% são introduzidas. O percentual de sementes de azevém, produzidas no Estado, contaminadas com sementes nocivas foi de 61,5% em 1978 e de 60,0% em 1979; e o de sementes int roduzidas no Estado foi de 45,6% em 1978 e de 29,4%em 1979. Foi observado que ent re as sementes originárias do RS destacaram -se com maior ocorrênci a em 1978 as espécies nocivas de Silene gallica, Setaria geniculata, Anthemis cotula, Digitaria adscendens e Echinochloa spp, enquanto que nas sementes int roduzidas desta caram-se Sida spp e Rumex spp; em 1979, na semente oriunda do Estado desta caram-se Amaranthus spp, Silene gallica e Setaria geniculata, enquanto que nas sementes introduzidas a maior ocorrência foi de Setaria geniculata, Echinochloa spp e Solanum spp.

Weed Problems of Grazing Lands and Control of Some Problem Weeds in the Markham Valley of Papua New Guinea

Abstract Weeds are a serious problem in this region during both the establishment of pastures and their productive period, causing serious reduction in growth, vigour, productivity and persistence. Factors responsible for weed infestation and possible methods of prevention and control are outlined in this paper. The biology and weed ecology of Cassia alata, Digitaria insularis, Jatropha gossypifolia, Mimosa spp, Sida spp, Solarium mauritianum and Solanum torvum are briefly described and detailed recommendations are given for the control of these weeds.

Herbicidas na cultura do feijão (Phaseolus Vulgaris L.): controle, fitotoxicidade e persistência no solo

A presente pesquisa foi conduzida através de dois ensaios com os herbicidas trifluralin, nitralin e EPTC em duas doses cada, procurando-se verificar o controle das plantas daninhas, observar os possíveis efeitos fitotóxicos à cultura após reaplicação no mesmo ano agrícola e determinar os possíveis resíduos no solo, no início do ano agrícola seguinte, que pudessem afe tar culturas rensiveis. O desenvolvimento da cultura foi observado por meio de pesos de matéria seca de folhas, caules e vagens, durante o ciclo, obtendo -se, também, a produção de vagens no fim do ciclo. A determinação dos possíveis residuos no solo, foi realizada através de bio-ensaios de radículas e caulículos, utilizando-se o sorgo (Sorghum bicolor (L.) Moench.) como planta teste. Houve um bom controle das plantas daninhas pelos herbicidas utilizados. A tiririca (Cyperus rotundus L.) só foi controlada pelo EPTC. O picão-preto (Bidenspilosa L.) e a guanxuma (Sida spp.) não foram controlados. Nenhum dos herbicidas apresentou fitotoxicidade à planta de feijão, mesmo após reaplicação no mesmo ano agrícola, e não foram constatados resíduos no ano agrícola seguinte no solo, após a reaplicação no ano agrícola anterior.

Aplicação de misturas de diuron com MSMA, e com paraquat, no controle de plantas daninhas de folhas largas em cultura de algodão (Gossypium hirsutum L.)

Em ensaio de campo conduzido em 1975/76 procurou-se avaliar a ação de misturas de MSMA com diuron e de paraquat com diuron, aplicadas em pós-emergência, em jato dirigido, em duas épocas diferentes, no controle de algumas plantas daninhas de folhas largas em algodão: carrapicho- do-campo (Acanthospermum australe (Loef) O. Kuntze) , falsa-poaia (Borreria ala ta (Aubl) DC), poaia-branca (Richardia brasiliensis Gomez ) e guanxuma (Sida spp) . A vegetação natural da área do ensaio era formada ainda pela gramínea capim-de-colchão (Digitaria sanguinalis (L.) Scop ). Os resultados mostraram que as misturas de 2,00 kg e 2,70 kg/ha de MSMA com, respectivamente 0,30 kg e 0,40 kg/ha de diuron, e a mistura de 0.60 kg/ha de paraquat com 0,60 kg/ ha de diuron, foram eficientes no co ntro le daquelas dicotiledôneas, e também no da gramínea. Todos os tratamentos provocaram leves sintomas de fitotoxicidade nos algodoeiros, mas desapareceram depois e não prejudicaram o desenvolvimento vegetativo das plantas, assim como a produção de algodão em caroço.