Shrimp Culture Research Articles

Mplementation of Extreme Learning Machine for Water Quality Control in Vannamei Shrimp Ponds

The application of science and technology knowledge is crucial in supporting the Indonesian Government's program to increase the production of Litopenaesus Vannamei shrimp. This research collaborates with shrimp pond farmers to develop technology that supports the cultivation of vaname shrimp. The water quality affect the harvest results, and the water parameters such as pH, dissolved oxygen (DO), alkalinity, salinity, and temperature should be monitored and adjusted if the parameters exceed the predetermined limits. We have developed an Extreme Learning Machine-based water quality management system tailored to the geographic conditions of Indonesia. This tool uses sensors to read data from the pond water, which is then processed by a microcontroller and displayed in a web-based information system. This tool helps farmers determine the water conditions and condition it accordingly. Based on experimental result error dari data training adalah 0.0001 dan error pada data testing yaitu sebesar 0.1851, it can be seen the Extreme Learning Machine has good performance for this research.

Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus

AbstractIn this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain reaction (PCR), DHPV was detected in all shrimp samples (83 of 83); among these, 41 samples were detected with WSSV. Amplicon sequencing and phylogenetic analyses indicated that all DHPV samples were classified as a novel genotype of DHPV. Additionally, the collected squid, polychaeta, and crab were also confirmed to be detected with novel genotypes of DHPV. By WSSV quantitative PCR, the average concentrations of viruses detected in shrimp and pond water samples were 2.58 × 107 copies/μL and 1.27 × 102 copies/μL, respectively. Simultaneous detection of WSSV and DHPV was observed in shrimp, pond water, and crab samples, and co‐infection with both viruses in shrimp could be demonstrated by histopathological analysis. Viral infection can result in significant economic losses in shrimp production. Therefore, precise monitoring and management of WSSV and DHPV prevalence in pond water, live food, and habitats are essential to preventing the spread of viral diseases in the Korean shrimp culture industry.

Effects of combined rice flour and molasses use on the growth performance of Pacific white shrimp (Litopenaeus vannamei Boone, 1931) applied biofloc technology

A 63-day completely random experiment with three replications was carried out to compare the effects of five different combination ratios of rice flour (R) and molasses (M) on the growth and survival rates of Pacific white shrimp (Litopenaeus vannamei Boone, 1931) postlarvae applied biofloc technology. Five biofloc (BF) treatments, including R90-M10, R70-M30, R50-M50, R30-M70, and R10-M90, formed with the addition of different combination ratios of rice flour and molasses, i.e., 90% R+10% M, 70% R+30% M, 50% R+50% M, 30% R+70% M, and 10% R+90% M, respectively, with C/N ratios of 15:1, and a control (neither rice flour nor molasses applied) was randomly arranged into the 18 plastic tanks of 1.0 m3 volume (with 0.5 m3 of water) each tank and salinity of 15‰. The postlarvae (0.095 g) were stocked into the tanks at a 150 ind. m−3 density and fed pelleted feed (40% protein). There was an improvement in growth (FMW, WG, DWG, and SGR) for all treatments. Besides, treatments with more than or equal to 30% molasses have improved SR, FCR, and FB. Especially the highest SR (94.2%) was obtained at the R70-M30, which perhaps created the highest FB (1.435 kg m−3) in this treatment. The lowest FCR (1.28) was also observed in the R70-M30 and significantly differed from the control and other treatments. Besides, water quality parameters were within the ranges recommended for Pacific white shrimp health during the experimental period. Our findings indicated the benefits of shrimp culture using the BF system when different combined ratios of rice flour and molasses were applied, of which a ratio of 70% rice flour and 30% molasses was considered as the best.

A novel medium for long-term primary culture of hemocytes of Metapenaeus ensis

The development of a suitable shrimp cell medium is essential for achieving a long-term cell culture and finite cell line of shrimps routinely. In this study, we have successfully developed an optimal shrimp cell medium that can be used for long-term in vitro culture and continuous subculture of the hemolymph cells (or hemocytes) of greasyback shrimp Metapenaeus ensis, designated as MeH cells, by shrimp serum-based and supplements-based optimization of the basic and growth medium. In this article, we have focused on the details for the preparation of the optimal shrimp cell medium by diluting and mixing of various stock solutions as well as the methods for isolation and primary culture of MeH cells.•A novel shrimp cell growth medium is developed for long-term shrimp hemocytes culture.•The preparation method of shrimp cell growth medium is successfully established.•Obvious cell activity and proliferation potential of isolated shrimp cells can be maintained beyond 30 days.

Protective effects of indigenous lactic acid bacteria in Artemia salina challenged with Vibrio parahaemolyticus: an in vitro and in silico approach

ABSTRACT Aquaculture is one of the fastest-growing sectors of the food industry and vibriosis is a major disease affecting finfish and shellfish production capabilities. In recent years, probiotics specifically lactic acid bacteria (LAB) have proven to be an environmentally friendly alternative to antibiotics to maintain aquatic animal health. In this study, five strains of indigenous LAB isolated from traditional and non-traditional sources were evaluated for their potency in the prevention of vibriosis in brine shrimp, Artemia salina as a model organism. The LAB were well tolerated at all doses, and no negative effects on the hatching ability of brine shrimps were observed when exposed to Lactiplantibacillus plantarum KCFe63 and Limosilactobacillus fermentum NCCu21. Furthermore, all the tested LAB were able to protect the brine shrimp from the pathogen Vibrio parahaemolyticus under co-exposure and pre-exposure conditions. Molecular docking analysis revealed a high binding affinity of common probiotic metabolites lactic, butyric and propionic acids to the PirAvp and PirBvp proteins of V. parahaemolyticus which can prevent toxin formation and thereby acute hepatopancreatic necrosis disease (AHPND). Overall results suggest that two strains, L. plantarum KCFe63 and L. fermentum NCCu21, are suitable candidates to reduce the incidence of vibriosis and AHPND during brine shrimp cultivation.

Effectiveness of dietary heat-killed Bacillus subtilis harboring plasmid containing 60 copies of CpG-ODN 1668 against Vibrio harveyi in Penaeus vannamei.

The cost of the purification process hinders the extensive use of cytosine phosphate guanosine-oligodeoxynucleotides (CpG-ODNs) for shrimp culture. Therefore, this study used a shuttle vector plasmid to carry 60 copies of CpG-ODN 1668 (pAD43-25_60CpG), which can replicate in Escherichia coli and Bacillus subtilis strain RIK1285. The first experiment used a reverse gavage procedure to deliver a substance (PBS [CK], pAD43-25 [P0], and pAD43-25_60CpG [P60], respectively) directly into the anterior midgut of Penaeus vannamei and transcriptome sequence analysis with a reference genome was performed to examine the expression of well-known immune-related genes. The results showed that the expression levels of immune-related genes in P60 group were significantly increased, particularly those associated with AMPs. In addition, using RT‒qPCR, the expression levels of AMP genes (LvALF, LvPEN-2, and LvPEN-3) in the P60 group may vary depending on the tissue and time point. The second experiment used dietary supplementation with three kinds of heat-killed B. subtilis (HKBS, HKBS-P0, and HKBS-P60) in 28 days of feeding experiments. The results showed that dietary supplementation with HKBS-P60 did not significantly improve shrimp growth performance and survival. However, on days 14 and 28 of the feeding regimens, alkaline phosphatase (AKP) and acid phosphatase (ACP) activity were considerably higher than in other treatments. In addition, following infection with Vibrio harveyi, AKP and ACP activity in the HKBS-P60 group was significantly higher than in other treatments, particularly at the early stage of bacterial infection. Moreover, HKBS-P60 was found to be better protected against V. harveyi infection with lower cumulative mortality (60%) compared to HKBS (90%) and HKBS-P0 (100%) at 7 days after infection. Overall, these findings confirmed that P60 could increase immunological responses in the shrimp midgut, and HKBS-P60 could be used as an effective tool to enhance the immune response and disease resistance in shrimp.

Insight into three water additives: Revealing the protective effects on survival and stress response under cold stress for Pacific white shrimp Litopenaeus vannamei.

The reproduction, development and growth of shrimp were hindered by cold stress, and even death was caused in severe cases. Moreover, huge economic losses to the shrimp aquaculture industry were caused every year by cold currents. The purpose of this study was to investigate the potential protective effects of water additives on the cold stress resistance of Pacific white shrimp (Litopenaeus vannamei) and their ability to improve the survival and stress response of the shrimp. Three potential cold-resistant additives adenosine triphosphate (A), soybean phospholipid (SP) and Clostridium butyricum (CB) on Pacific white shrimp under cold stress were added to the water with three concentrations for each additive. The mortality, activities of antioxidation enzymes and expression of anti-stress related genes in each group under cold stress were detected. The results showed that the cumulative mortality of low concentration for adenosine triphosphate (AL) and soybean phospholipid (SPL), medium concentration for soybean phospholipid (SPM) and high concentration for Clostridium butyricum (CBH) groups were significantly lower than that of the control (C) group when temperature maintained at 13°C for 6 days. Total antioxidant capacity (T-AOC) content in shrimp plasma was significantly higher, while malondialdehyde (MDA) content was significantly lower than that in the C group. Gene expression analysis showed that 0.4mg/L of adenosine triphosphate could regulate the immune defense ability and decrease apoptosis level of Pacific white shrimp under cold stress. Soybean phospholipid (2mg/L) could enhance the immune ability of hepatopancreas, and Clostridium butyricum (10mg/L) could significantly increase the expression of stress-related genes in shrimp intestine. Overall, these findings suggested that adenosine triphosphate and soybean phospholipid have the potential to be used as cold-resistant additives in Pacific white shrimp culture. This study provided valuable insights into addressing the problem of cold stress in shrimp culture.

Metagenomic Study on the Influence of Enterocytozoon hepatopenaei (EHP) Infection on the Gut Microbiota in Penaeus vannamei

Background: Enterocytozoon hepatopenaei (EHP), infects P.vannamei and causes hepatopancreatic microsporidiasis associated with severe growth retardation in shrimp culture. The influence of EHP in the shrimp gut microbiota is poorly studied, and this would be an interesting area to investigate. In this study, metagenomic approach was followed to compare the overall species richness of the gut microbiota in healthy and EHP-infected P.vannamei. Methods: Bacterial genomic DNA from gut samples of EHP-infected and healthy shrimps were profiled for the 16S rRNA gene, targeting the V3-V4 conserved region. Operational Taxonomic Units (OTUs), were identified and clustered together with a cutoff of 97% identity using UCLUST. The OTUs were then used for the computation of alpha diversity and beta diversity for each sample. Result: Gut samples from EHP-infected shrimp showed lower bacterial abundance throughout the family, class, order, and genus levels. This research also highlights that EHP not only affects the hepatopancreas of the shrimp, but it also has the ability to affect the shrimp gut, predisposing them to other opportunistic infections.

Study of trophic interactions of green mussels (Perna viridis) as bioremediation of waste for cultivation of windu shrimp (Penaeus monodon) in Gampong Alue Naga, Banda Aceh: application of IMTA system (Integrated Multi-Trophic Aquaculture)

Residual organic matter from the cultivation of Windu Shrimp (Penaeus monodon) can cause a decrease in the quality of cultured water. This study aims to examine the trophic interactions of Windu Shrimp (Penaeus monodon) and Green mussels (Perna viridis) in a polyculture system including observing growth performance, survival rate and bioremediation level of green mussels. This research was conducted in April – September 2022 in Gampong Alue Naga, Banda Aceh. Sample biota was Windu Shrimp (Penaeus monodon) (PL20) with an average length of 1.2 cm, and green mussels (Perna viridis) obtained from nature with a shell size range of 3 cm – 5 cm. Research on species composition per container volume in a polyculture system used a completely randomized design method with 4 treatment levels and 3 replications. The treatments in this study were differences in stocking density of green mussels A (0 ind/m2), B (20 ind/m2), C (40 ind/m2), and D (60 ind/m2). In this study, the number of windu stocked was the same, namely 60 fish/container with a container size of 1x1 meter. The results showed that the difference in stocking density of green mussels had a significant effect (p<0.05) on absolute growth and survival rate of Windu shrimp but had no significant effect on absolute weight and daily growth rate of Windu shrimp (p>0.05). The average daily growth rate (SGR) ranges from 10.54% - 11.98%. The lowest survival rate value was found in treatment B, which was 26.11±10.04 and the highest survival rate value is found in treatment C of 73.33±26.6. The best treatment in this study was the stocking density of green mussels of 40 ind/m2. Green mussel filtration rate ranged from 0.6 to 1.7 L/day. Application of multispecies cultivation system (polyculture) is better than monoculture system based on growth performance and filtration rate of green mussels (Perna viridis).

Meta-Analysis: The effectiveness of artificial diets to increase on survival rate of vannamei shrimp (Litopenaeus vannamei)

The use of artificial diets for shrimp culture has been widely used at this time, but it was not yet known how large the effect of artificial diets on the survival rate of Litopenaeus vannamei. This study aims to determine the effectiveness of artificial diets on on the survival rate of Litopenaeus vannamei by meta-analysis research. The measurement results obtained z-value of 5.336 and p-value of 0.001, this p-value was smaller than the significance value of 5% (0.05). Therefore, at a significant level of 95%Cl the correlation between artificial diets and the survival rate of vannamei shrimp was significant with a minimum confidence limit of 0.192 and a maximum of 0.343. Based on Egger’s test, the z-value which was the magnitude of the regression coefficient is 1.714, while the p-value of 0.086 is greater than 0.05 which indicates that the selection of articles and the results of the study were not indicated by publication bias. In addition, the p-value > 0.05 indicates that the funnel plot was symmetrical. Therefore, the results of this study are very useful theoretically in the development of artificial diets for the survival of vannamei shrimp.

CORRELATION BETWEEN WATER QUALITY PARAMETERS AND VIBRIO SP. BACTERIA CONTENT IN TRADITIONAL VANNAMEI SHRIMP (LITHOPENAEUS VANNAMEI) CULTURE

Vannamei shrimp is a species of shrimp that is frequently cultivated using conventional methods. This is because vannamei crustaceans have favorable prospects and profits. Traditional Vannamei shrimp cultivation has great development potential, but preventing parasites and diseases that originate in the pond water when cultivating shrimp is impossible. The incidence of disease is closely correlated with parameters of water quality. Pathogenic microorganisms, specifically the Vibrio sp. bacteria, are responsible for one of the prevalent maladies affecting vannamei shrimp farms. Vibrio sp. bacteria produces vibriosis, the clinical symptoms of brownish-red gills, a crimson uropod and abdominal telson, and a sluggish swimming speed. This study aimed to determine the correlation between Vibrio sp. bacteria content and water quality parameters in conventional vannamei shrimp (Lithopenaeus vannamei) cultivation. This study employed exploratory methods and purposive sampling for its sampling. Isolation of microbes using selective media containing TCBSA. The colony abundance of Vibrio sp. bacteria was calculated using the Total Plate Count method. The results showed no significant correlation between the quality of pond water and the abundance of Vibrio sp. bacteria colonies in the traditional system of vannamei shrimp (Lithopenaeus vannamei) cultivation, with a correlation value of &gt; 0.05.

Crop diversification as a household livelihood strategy under environmental stress. Factors contributing to the adoption of crop diversification in shrimp cultivation and agricultural crop farming zones of coastal Bangladesh

Environmental stressors, potentially aggravated by climate change, pose significant challenges to households whose livelihoods rely primarily on crop production in agriculture or aquaculture, particularly in countries of the Global South. In this context, diversified farming systems, or crop diversification, have been discussed as one adaptation strategy of smallholder farming households to reduce their livelihood vulnerability and increase farm resilience. In coastal Bangladesh, livelihoods based on cultivation of shrimp, prawn, fish, paddy (rice) and other crops are likely to become more vulnerable with accelerated sea level rise, extreme flooding events, cyclone activity, river bank erosion and salinization. While crop diversification in Bangladesh is still low overall, it has been increasing. To understand the factors driving the uptake of diversified cropping in different farming production contexts and allow for policy measures addressing regionally specific needs, we explore the (relative) impact and significance of relevant factors contributing to the adoption of crop diversification practices for (i) shrimp cultivation and (ii) agricultural crop farming zones. We specifically include variables representing subjectively perceived risks to study their role in climate change adaptation. Our findings are based on a quantitative household survey (n = 1188) in nine purposefully selected unions across the Bangaleshi part of the Ganges-Brahmaputra-Meghna delta to capture the diversity of the region. Our results show that the prevalence of crop diversification measures is relatively high in the shrimp cultivation zone, but relatively low in the agricultural crop farming regions. In sum, the results show that even though direct economic factors seem to be (still) more important for diversification decisions, our study also suggests that perceived environmental threats and changes can have a favourable influence on crop diversification and should hence be included in studies investigating causes for land use changes. Yet, there are significant differences with regard to the influence of specific factors on the likelihood to diversify in the two respective zones. We conclude that greater uptakes of agriculture and aquaculture diversification could represent a promising and more sustainable approach for smallholders in coastal Bangladesh given appropriate supportive institutional conditions and measures that address specific needs of local communities. A “one-size-fits-all” approach to pushing crop diversification is unlikely to produce adequate and sustainable results.

The Role of Social Capital in the Development of Shrimp Cultivation Pond Business

Shrimp cultivation is a priority in the aquaculture sector, and the development of aquaculture in Indonesia to improve the national economy; something that cannot be ruled out is the element of social capital. This social capital is related to various forms of cooperation or collective action in society to achieve better life goals, which are supported by values, norms, trust, and networks. To overcome the various obstacles that arise in the implementation of the development and expansion of the shrimp pond business, group members or the community of Pedes District, Karawang Regency, cooperation and active participation from the community are needed. The component of social capital in the form of trust is the basis for forming social interaction. In addition to the trust that must exist between farmers, there is a desire to participate and form a network, both among fish farmers and with other parties such as investors and collectors or companies that accommodate their crops. So, social capital plays a role in developing shrimp farming in Karawang Regency. The data collection technique used is observation and in-depth interviews with a key informant who is an elder figure (star person) and three representatives of aquaculture farmers who have been cultivating for the last ten years so that primary data is obtained from informants regarding the role of social capital in developing aquaculture businesses. Shrimp Ponds in Pedes District, Karawang Regency.

Total ammonia nitrogen removal and microbial community dynamics in an outdoor HDPE-lined shrimp pond with no water discharge

This study assessed the characteristics of a zero-discharge outdoor high-density polyethylene (HDPE)-lined demonstration aquaculture pond (400 m2) in terms of water quality variables, total ammonia nitrogen (TAN) removal and oxygen net primary production (NPP) indices, and photosynthetic microbiome over a 76-day crop cycle. The shrimp harvest yield was 4.1 t/ha/crop with an average daily gain (ADG) of 0.15 g/day and a feed conversion ratio (FCR) of 1.49. During shrimp cultivation with a semi-intensive stocking density (40 shrimp/m2), there were low levels of TAN (<1.01 mg-N/L), nitrite (<0.21 mg-N/L), and nitrate (<2.79 mg-N/L). Ammonia removal was mainly mediated by photoautotrophic uptake, as evidenced by the consistent estimated TAN uptake rate of 0.05 ± 0.02 g-N/m3 h, which was calculated based on photosynthesis. The overall TAN removal rate of 0.10 ± 0.08 g-N/m3 h was maintained by suspended microorganisms in water, which exceeded that of microorganisms attached to the HDPE liner surface. A positive oxygen NPP of 0.24–1.16 g-O2/m3 h during light availability indicated prevailing autotrophic conditions. The bacterial community in the water was dominated by Phyla Pseudomonadota (Proteobacteria; 16–32%), Bacteroidota (Bacteroidetes; 15–29%), Cyanobacteria (7–35%), and Actinomycetota (Actinobacteria; 3–39%). The chloroplast amplicon sequence variants (ASVs) indicated abundances of Bacillariophyta (27–97%) and Chlorophyta (0.4–67%). High centric diatom populations (Bacillariophyta) were detected throughout cultivation, while the proportion of green algae (Chlorophyta) was positively correlated with increased TAN availability when feeding was increased. The findings enabled estimations of TAN removal and oxygen NPP rates and provided an understanding of correlations between environmental variables and bacterial and phytoplankton populations in the outdoor lined shrimp pond.

Effects of Various Nitrogen Sources on the Growth and Biochemical Composition of &lt;I&gt;Chlorella&lt;/I&gt; sp.

Highlight Research Each species of microalga has a preferable nitrogen source for their optimal growth. The nitrogen sources in the grown media affected the growth rate and biochemical composition of Chlorella FNUB01. (NH2)2CO (urea) was found to be the best alternative nitrogen source for Chlorella FNUB01. For producing 1 g of Chlorella FNUB01, the use of urea reduced the cost of medium by 72.6%. Abstract Chlorella sp. is a potential microalgae species to be produced commercially for feed, growth accelerator, and immuno-modulator in fish and shrimp culture. This study aimed to evaluate the various nitrogen sources on the growth, biomass production, and biochemical composition of Chlorella sp. FNUB01. The nitrogen sources used in this study were urea (NH2)2CO, potassium nitrate (KNO3), and ammonium nitrate (NH4NO3). Sodium nitrate (NaNO3) was used as a control as it is a part of the commercial medium BG-11. Generally, the sources of nitrogen in the media affected the growth and chemical composition of Chlorella sp. FNUB01. This green microalga grew better in the urea-containing medium which accounted for 1.5 times the concentration of that cultured in BG-11 (40 x106 cells. mL-1). Meanwhile, this microalgae species experienced the lowest growth when cultured in NH4NO3-containing medium. The biomass productivity of Chlorella sp. FNUB01 cultured in urea (0.93 g.L-1) was comparable to those grown with NaNO3 as the N source. A similar pattern was recorded for protein, chlorophyll, and carotenoid content as these biochemical contents were affected by N availability in the medium. Urea was an alternative low-cost N source for the culture of Chlorella sp. FNUB01. Replacement of NaNO3 with urea could reduce the cost of the medium by 72.6%.

Microalgae-mediated tandem culture of shrimp and bivalve: an environmental and health co-benefits solution for phosphorus recovery and emission reduction

Phosphorus (P) accumulation in aquaculture systems is damaging our environment beyond acceptable levels. Devising strategies to potentially recover P from aquaculture systems in a reusable bioresource form is paramount and aligns with circular economy policies. In this study, we constructed two culture models, monoculture (Mon) and tandem culture (Tan), using Exopalaemon carinicauda and Mercenaria mercenaria. By monitoring the performance of rearing organisms, P dynamic patterns, and pollutant emissions, we found that: i) Compared to the Mon system, the Tan system demonstrated no differences in the performance of E. carinicauda and M. mercenaria, suggesting that the Tan model was viable in terms of fishery yield; ii) P in the Tan system could be efficiently recovered and removed from water and sediment, as indicated by the lower phosphate concentration in water (0.01 mg L−1), and the decrease in labile P in surface sediment (from 0.04 to 0.02 mg L−1). A combination of assimilatory and dissimilatory processes, mediated by phototrophic (bait-microalgae) and heterotrophic organisms (bivalves), appeared to be the primary mechanism for P utilization and removal; iii) The Tan system reduced pollutant emissions four times lower than the Mon system due to its minimal tailwater discharge (10%, 230 L). The emissions of total P, phosphate, total organic carbon, ammonium, and chemical oxygen demand from the Tan systems were 19 mg m−2 d−1, 2 mg m−2 d−1, 2 g m−2 d−1, 38 mg m−2 d−1, and 11 g m−2 d−1, respectively, 1.3, 1.7, 1.4, 1.3, and 1.2 times lower than those from the Mon systems. The eco-friendly Tan culture model fully exploited the resources of pond culture, a solution with environmental and health co-benefits for P recovery and emission reduction.

Analysis of Pond Revitalization and Land Suitability for Vaname Shrimp (Litopenaeus vannamei) and Milk Fish Cultivation Tradisonally Policultural: A Case Study in Muara Kintap Village, Kintap District, Tanah Regency

Shrimp and milkfish are the main commodities of aquaculture in ponds, which have considerable development potential in almost all coastal areas in Indonesia. Based on the description above, to revitalize ponds in the village of Muara Kintap, Kintap District, the research aims to analyze the efforts to revitalize the aquaculture ponds of vannamei shrimp (litopenaeus vannamei) and milkfish in a polyculture manner based on water quality analysis in the village of Muara Kintap, Kintap District, Tanah Laut Regency, South Kalimantan Province. Implementation is planned for June 2023, with the location of the research being in the community pond area of Muara Kintap Village, Resistant Laut District, South Kalimantan Province. Business revitalization of vannamei shrimp (litopenaeus vannamei) and milkfish aquaculture ponds in polyculture based on water quality analysis in Muara Kintap Village, Kintap District, Tanah Laut Regency, Kalimantan, obtained an average R/C ratio for traditional vannamei shrimp farming business, which is worth 3.19, which means the aquaculture business Traditionally polyculture vannamei shrimp is efficient in the use of business costs, because every 1 unit of currency (Rp. 3.19) issued as the cost of the vannamei shrimp farming business will provide business revenue, and based on the land suitability analysis resulting from the interpolation of all parameters it can be concluded that at the research location it was stated that it was suitable to very suitable. The community of Muara Kintap village, Tanah Laut Regency, South Kalimantan Province.

A Review on Herb Utilization in Vannamei Shrimp Cultivation

Aquaculture activities play a crucial role in boosting fishery production. Among the various commodities, Vannamei shrimp (Litopenaeus vannamei) stands out as a leading choice due to its competitive pricing and the ability to conduct mass production with high stocking densities. However, shrimp farming has encountered challenges, particularly the occurrence of diseases that often lead to reduced production levels. These diseases typically arise from the shrimp's weakened condition and suboptimal rearing conditions. To address this issue, incorporating several herbal plants, such as noni fruit, ginger, and garlic, into the feed has emerged as an alternative to enhance shrimp immunity. Through an extensive literature search, optimal doses for these herbal additives were identified. Noni fruit demonstrated the best performance at a 6% dose, resulting in a feed conversion ratio (FCR) of 1.1 and a survival rate of 95%. Garlic performed well at a 5% dose, achieving an FCR of 1 and a survival rate of 98%. Similarly, ginger showed promising results at a 3% dose, with an FCR of 1.18 and a survival rate of 98%. The findings of this study emphasize that incorporating herbs into the feed can serve as a viable solution to enhance productivity in Vannamei shrimp farming.

Targeted metagenomics using bait-capture to detect antibiotic resistance genes in retail meat and seafood.

Metagenomics analysis of foods has the potential to provide comprehensive data on the presence and prevalence of antimicrobial resistance (AMR) genes in the microbiome of foods. However, AMR genes are generally present in low abundance compared to other bacterial genes in the food microbiome and consequently require multiple rounds of in-depth sequencing for detection. Here, a metagenomics approach, using bait-capture probes targeting antimicrobial resistance and plasmid genes, is used to characterize the resistome and plasmidome of retail beef, chicken, oyster, shrimp, and veal enrichment cultures (n = 15). Compared to total shotgun metagenomics, bait-capture required approximately 40-fold fewer sequence reads to detect twice the number of AMR gene classes, AMR gene families, and plasmid genes across all sample types. For the detection of critically important extended spectrum beta-lactamase (ESBL) genes the bait capture method had a higher overall positivity rate (44%) compared to shotgun metagenomics (26%), and a culture-based method (29%). Overall, the results support the use of bait-capture for the identification of low abundance genes such as AMR genes from food samples.

Effect of a Bacillus Probiotic Compound on Penaeus vannamei Survival, Water Quality, and Microbial Communities

Given the widespread use of commercial probiotics in aquaculture, it is important to evaluate the quality and environmental effects of these additives. Here, the effects of a Bacillus probiotic compound, BG4, on Penaeus vannamei survival rate, water quality factors, and microbial communities were assessed. An analysis of the BG4 powder confirmed the content of probiotic bacteria: 50.2% Bacillus licheniformis, 48.4% Bacillus subtilis, and 1.4% Bacillus amyloliquefaciens, consistent with the information on the product packaging. The effective bacterial quantity (1.1 × 109 CFU/g) was higher than that indicated on the product label (2 × 108 CFU/g). BG4 was added to a sterilized aquaculture water system, according to the indicated bacterial quantity; after 7 days, the maximum ammonia nitrogen, phosphate, and chemical oxygen demand degradation rates were 36.3%, 28.9%, and 15.2%, respectively. In the shrimp culture experiment, the survival rate of P. vannamei and water quality did not differ significantly between the BG4 and control groups. The bacterial quantity and high-throughput sequencing results indicated that Bacillus content in BG4 group decreased continuously from 3.5 × 104 CFU/mL to 6.9 × 102 CFU/mL. To ensure the desired effect of probiotics in commercial aquaculture applications, additional quality evaluations and scientific assessments are needed.