The hydrolysis rate is enhanced by temperature and total solid (TS) content during anaerobic digestion (AD). However, methanogenesis can be easily inhibited under these same conditions. Therefore, a balance point may exist between hydrolysis and methanogenesis by regulating these parameters. This study explored the AD of chicken manure and corn straw at different temperatures (35℃, 45℃, and 55℃) and TS contents (6%, 8%, 10%, and 12%). Results indicate that as TS content increases, the optimum temperature shifts from thermophilic to mesophilic. This could be explained by excessive hydrolysis under higher temperatures and TS may hinder methanogenesis due to ammonia accumulation. At 8% TS, 45°C achieves the highest methane production, challenging the notion that this temperature range lacks efficient gas production. This indicated that the optimal temperature is not fixed and should be evaluated alongside other operational parameters, especially in industrial AD. Elevated temperatures and higher TS content boosted glycolysis metabolism, amino acid metabolism, and hydrogenotrophic pathways but restricted pyruvate metabolism and acetoclastic methanogenesis, leading to reduced methane production. Compared to bacterial adaptation, methanogens showed less resilience to environmental changes.
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