Severe Damage Research Articles

Overexpression of SULT1E1 alleviates salt-processed Psoraleae Fructus-induced cholestatic liver damage

ObjectiveSalt-processed Psoraleae fructus (SPF) is widely used as a phytoestrogen-like agent in the treatment of osteoporosis. However, due to improper clinical use or misuse, resulting in liver damage. In this study, network pharmacology was employed to analyze the mechanism of cholestatic liver damage. An adeno-associated virus overexpressing SULT1E1 (rAAV8-SULT1E1) was constructed and the hepatotoxicity of SPF, psoralen, and isopsoralen was determined. MethodsBy utilizing three databases inclding TCMSP, TCMID, and BATMAN- TCM, the targets of the three databases were summarized, and a total of 45 psoralen compounds were included. Network pharmacology analysis was then performed. The adenoviral vectors were injected into the tail vein of C57BL6 mice to elucidate the role of SULT1E1 in SPF-induced cholestasis-mediated hepatotoxicity in vivo. SPF (10 g/kg), psoralen, and isopsoralen (50 mg/kg each) were intragastrically administered to mice for 30 d. B-ultrasound and samples were collected and examined for follow-up experiments. ResultsA total of 854 targets were predicted for 45 active components, with 151 cholestasis-mediated hepatotoxicity-related disease targets obtained for SPF. A total of 126 pathways were enriched based on KEGG pathway analysis, with the “estrogen signaling pathway” identified as one of the top 20 pathways. In terms of pathological hepatic changes, treated mice had visually swollen hepatocytes, dilated bile ducts, and elevated serum biochemical markers, which were more prominent in mice treated with isopsoralen than in those treated with other compounds. Notably, the overexpression of SULT1E1 could reverse liver damage in each treatment group. B-ultrasound was used to observe the size of the gallbladder in vivo. The size of the gallbladder was found to significantly increase on day 30 after treatment in the SPF-, psoralen-, and isopsoralen-treated groups, especially the SPF group. Compared with the expression levels in the negative control group (rAAV8-empty+con), the expression levels of FXR, Mrp2, Bsep, SULT1E1, SULT2A1, Ntcp, and Nrf2 decreased, whereas those of CYP7a1 and IL-6 increased in the SPF-, psoralen-, and isopsoralen-treated groups. ConclusionThe overexpression of SULT1E1 could alleviate the decreased or increased expression of indicators, indicating that SULT1E1 is an important target gene for SPF-induced liver damage. The severity of liver damage was significantly lower in the rAAV8-SULT1E1 groups than in the rAAV8-empty groups.

2'-Fucosyllactose ameliorates aging-related osteoporosis by restoring gut microbial and innate immune homeostasis.

Aging-related osteoporosis is considered as a serious public health concern for middle-aged and elderly people, with an intricated pathogenesis including the recently identified aging-induced immunological dysfunction and gut microbial disorder. The intervention based on dietary prebiotics is recommended to retain bone health and postpone the progression of osteoporosis. As a well-defined prebiotic, 2'-fucosyllactose (2'-FL) has been thoroughly validated with positive effect on systemic health and was proposed in this study to unveil its intervention on aging-related osteoporosis, as well as the underlying mechanisms involving the gut microecology and innate immunity. The effects of dietary 2'-FL on osteoporosis phenotypes were identified by evaluating the severity of bone loss and microstructure damage in natural aging mice. The mechanisms relying on innate immune profile, intestinal barrier function, and gut microbial homeostasis, were analyzed to elucidate the signaling axis. The detailed molecular signaling was validated based on LPS-stimulated RAW 264.7 murine macrophages. The results indicated that 12-week 2'-FL intervention retrieved bone loss and microstructure damage in natural aging mice. Also, 2'-FL alleviated aging-induced colonic inflammation, gut barrier dysfunction, and abnormal expression of intestinal tight-junction protein. The impact of 2'-FL treatment on the aging-induced gut microbial dysbiosis was validated by restoring gut microbiota diversity, recovering the abundance of Bifidobacterium, Prevotellaceae and Akkermansia, and inhibiting the growth of Stenotrophomonas. Flow cytometry analysis revealed changes in dendritic cell (DC) and macrophage subsets with age, and a decrease in M1-polarized macrophages was observed in 2'-FL-treated aged mice and RAW264.7 cells potentially through the interaction with toll-like receptor 4 (TLR4) to suppress NF-κB signaling and the secretion of proinflammatory factors. These findings highlight the preventive effect of 2'-FL on aging-associated osteoporosis by regulating gut microbial homeostasis and innate immune responses.

Multistable Twist Metastructures with Enhanced Collapsibility and Multidimensional Programmability

Multistable metastructures with compression-twist coupling offer promising applications in reusable protective devices, deployable structures and reconfigurable robotics. However, existing designs based on either Kresling origami or truss-based mechanisms, suffer from limited deformability, due to the accumulation of bending deformation in creases or trusses. Herein, we propose a novel multistable twist metastructure by integrating hinged beams with Kresling-inspired trusses. A two-step procedure, combining 3D printing and interlocking assembling, is utilized to fabricate the multistable twist samples. This multistable twist mechanism leverages the elastic instability and shape reconfiguration of hinged beams, enabling transitions between stable configurations with minimal bending in the trusses. This approach achieves exceptional collapsibility with a reusable maximum allowable compression up to 80% of the structural height. Additionally, the compression-twist coupling of trusses protects hinged beams from severe tensile damage. Furthermore, our strategy offers multidimensional programmability. Geometric design tailors configuration stability (i.e., multi/bistability, monostability, monotonicity), while arraying method controls deformation modes. This culminates in the realization of customized functions of impact resistance and vibration mitigation. Specially, by incorporating trusses, negative stiffness with loop hysteresis can be programmed to enhance energy dissipation, which facilitates to damping the impact and vibration. Experimental tests confirm the compatibility of excellent collapsibility, programmability and multifunctionality. This finding underscores the potential of such multistable metastructure with compression-twist coupling for designing next-generation reusable multifunctional devices.

Ionizing radiation has negligible effects on the age, telomere length and corticosterone levels of Chornobyl tree frogs.

The accident that occurred at the Chornobyl nuclear power plant (Ukraine, 1986) contaminated a large extension of territory after the deposition of radioactive material. It is still under debate whether the chronic exposure to the radiation levels currently present in the area has long-term effects on organisms, such as decreases in longevity. Here, we investigate whether current levels of radiation in Chornobyl negatively impact the age of the Eastern tree frog Hyla orientalis. We also explore whether radiation induces changes in an ageing marker, telomere length or the stress hormone corticosterone. We found no effect of total individual absorbed radiation (including both external and internal exposure) on frog age (n = 197 individuals sampled in 3 consecutive years). We also did not find any relationship between individual absorbed radiation and telomere length, nor between individual absorbed radiation and corticosterone levels. Our results suggest that radiation levels currently experienced by Chornobyl tree frogs may not be high enough to cause severe chronic damage to semi-aquatic vertebrates such as this species. This is the first study addressing age and stress hormones in Chornobyl wildlife, and thus future research will confirm if these results can be extended to other taxa.

Seismic performance and calculation method for plastic hinge length of RC pier reinforced with UHPC

Piers are highly vulnerable load-bearing components, and their seismic performance determines the integral seismic resistance of bridges to a certain degree. They play crucial roles in evacuation during earthquakes and post-earthquake rescue. The manner in which piers meet the seismic performance requirements after reinforcement has become a popular research topic. In this study, ultra-high-performance concrete (UHPC) and HTRB600E high-tensile reinforcements were used to expand the cross section of a cast-in-place square pier with severe bending damage. The earthquake-resistant behaviour indicators of the pier were compared and analysed by conducting pseudo-static tests on a reinforced concrete pier before and after reinforcement. These indicators included the hysteretic curve, skeleton curve, energy dissipation curve, rigidity degradation, residual displacement, and pier body curvature. Based on the results of the seismic reinforcement quasi-static tests, a finite element model (FEM) of the bridge pier was constructed using the ABAQUS software, and the accuracy of the FEM was averaged. The impact of the reinforcement layer height, thickness, longitudinal strength and axial compression ratio on the equivalent plastic-hinge length of the reinforced pier was analysed, and the formula for the equivalent plastic-hinge length of the reinforced pier was obtained based on multiple linear regression fitting. The analysis results demonstrated that the broadened section method, utilising UHPC and high-tensile reinforcement, significantly enhanced the flexural capacity, energy dissipation, and first rigidity of the bridge pier. The earthquake-resistant behaviour of pier was effectively restored and improved, and the seismic reinforcement effect was significant. The equivalent plastic hinge length of the pier after reinforcement is proportional to the height of the reinforcement layer, the thickness of the reinforcement layer, the strength of the longitudinal reinforcement, and inversely proportional to the axial compression ratio.

Metabolic and hepatic phenotypes in sarcopenic obesity and impact of bariatric surgery

Background & AimsSarcopenic obesity (SO) is associated with cardiometabolic disorders and steatotic liver disease and carries major health risks. We assessed the hepatic and metabolic clinical phenotype associated with SO in patients with obesity undergoing bariatric surgery (BS). We also evaluated whether weight-loss and metabolic improvement post-surgery differ between patients with and without SO. Methods972 consecutive patients from a single-center BS cohort who underwent whole-body dual-energy X-ray absorptiometry (DXA) and peri-operative liver biopsy were included. SO was diagnosed using the AIM-SO score, an AI-assisted unbiased clustering algorithm based on body composition. One-year post-surgery, 862 patients were reassessed for AIM-SO score changes. ResultsPre-operatively, 207 (21.3%) patients were diagnosed with SO. These patients had significantly higher prevalence of type-2 diabetes (T2D), arterial hypertension and obstructive sleep apnea (OSA) compared to patients without SO (all p≤0.003). Patients with SO had more severe liver damage: higher grades of moderate/advanced steatosis (64.2% vs. 47.3%), steatohepatitis (44.4% vs. 32.3%) and advanced fibrosis (12.1% vs. 6.0%) (all p≤0.01). One-year post-BS, 58.5% of patients had remission of SO. Patients with persistent SO exhibited less weight-loss than those with SO remission (–23.8 kg vs. –29.1 kg, p<0.001) and had lower rates of remission for T2D (41.9% vs. 69.8%), arterial hypertension (20.8% vs. 45.3%), and metabolic syndrome (47.6% vs. 75.0%) (all p≤0.009). ConclusionThe DXA-based AIM-SO score identifies patients with SO who are at greater risk of hepatic and cardiometabolic comorbidities, and predicts less favorable weight-loss and metabolic improvements post-BS.

Phenotypes of patients with lupus-associated pulmonary hypertension in the Chinese Han population: a cluster analysis.

Pulmonary hypertension (PH) is a severe complication of systemic lupus erythematosus (SLE). This study investigated the clinical features of patients with SLE-PH in China based on disease manifestations and organ involvement to define precise treatment of SLE and early prevention of complications. In total, 205 SLE-PH patients were included in this study. A cluster analysis was applied according to six clinical and serological variables to define different subgroups of patients. The survival rates of SLE-PH patients and risk factors that influenced prognosis were also compared and a clinical prediction model was developed for the diagnosis of associated lupus nephritis (LN). Patients were clustered in five groups. Patients in cluster 1 had severe renal damage (all patients had LN and had the highest creatinine and urea nitrogen and the lowest eGFR levels). Patients in cluster 2 had mild renal damage (more than half of the patients had associated LN and 87.5% had increased urinary protein levels but presented a lower degree of renal damage than those in the first group. Patients in cluster 3 had low-grade proteinuria (all patients had 24h urinary protein < 0.5g). Patients in cluster 4 had hematuria or urinary tubular damage (26% of patients had associated LN, but none of the patients had proteinuria. In cluster 5, patients barely had any major organ involvement. The clinical prediction model for a diagnosis of SLE-PH-LN was anti-dsDNA antibodies > 364.64IU/mL and neutrophil-to-leukocyte ratio (NLR) > 5.55. Our findings provide evidence indicating that SLE-PH presents varying clinical phenotypes and the treatment varies accordingly, suggesting the need for individualized treatment. Key Points • Clustered grouping of patients with SLE-PH is associated with renal injury. • This may be because PH and LN share a common pathogenesis. • The clinical prediction model for a diagnosis of SLE-PH-LN was anti-dsDNA antibodies >364.64 IU/mL and NLR >5.55.

Increased angiopoietin-1 improves nailfold capillary morphology in patients with systemic sclerosis

ObjectiveRaynaud's phenomenon is a common symptom of systemic sclerosis. We previously reported that elbow heating increases angiopoietin-1 in the fingertips and alleviates Raynaud's phenomenon. Angiopoietin-1 levels decrease in patients with systemic sclerosis with severe capillary damage. We aimed to conduct a prospective study to confirm whether the increase in angiopoietin-1 caused by heating modifies capillary morphology. MethodsThe left ring fingers of 19 patients with systemic sclerosis were monitored six times at 4-week intervals using capillaroscopy, during which both elbows were heated using disposable heating pads for 8 weeks. Blood samples were collected from the same fingertips four times—before heating, twice during heating, and once after heating—to measure angiopoietin-1. ResultsIn six patients, the peak increase in angiopoietin-1 occurred 4 weeks after the start of heating, whereas in seven patients, the peak value was observed 4 weeks after the termination thereof. No change in the density of the front-row capillaries was observed by capillaroscopy. The proportion of hairpin-shaped capillaries increased from 20.2 % during the preheating period to 26.6 % during the heating period (p = 0.00107). When a correlation coefficient of 0.6 or higher was set as significant, there was a strong correlation between changes in fingertip angiopoietin-1 levels and changes in the proportion of hairpin-shaped capillaries in six patients. ConclusionIncreased angiopoietin-1 levels in the fingertip due to elbow heating may improve the peripheral capillary morphology in patients with systemic sclerosis.

Study on the resistance of basalt fiber concrete to erosion of building structure by wind gravel flow

Concrete structures in the windy regions of the Gobi face constant erosion from wind gravel flow, leading to severe surface damage and significantly reduced durability. This paper investigates the erosion resistance of basalt fiber (BF) concrete under various erosion conditions using the air-flow sand injection method. The results indicate that adding 0.15% BF to concrete reduces its erosion rate by 20.7% to 21.5% compared to concrete without BF. Therefore, a BF content of 0.15% provides optimal erosion resistance for the concrete. The correct amount of BF bonds tightly with the cement mortar, forming a mesh structure inside the concrete that absorbs the kinetic energy of gravel impacts. The concrete erosion process can be divided into an early accelerated phase and a later stabilized phase. Throughout these phases, the erosion rate of concrete increases with the erosion angle (EA), erosion wind speed (EWS), and gravel flow rate (GFR). In particular, the erosion rate of BF15 concrete increased by 35.6 to 46.4mg/cm² when the EA changed from 15° to 90°. Notably, surface damage from low-angle erosion mainly consists of cut scratches, while high-angle erosion primarily results in erosion pits. As the grain size of the eroded gravel increases, the erosion rate of the concrete decreases. However, when a single large gravel particle impacts the concrete, it creates a larger and deeper erosion pit. Furthermore, the erosion rate of BF concrete exhibits a strong negative linear correlation with its mechanical properties, with an R² value exceeding 0.88. Moreover, erosion reduces both compressive and splitting tensile strengths of concrete. For BF15 concrete, however, the decreases are minimal: compressive strength drops by just 1.2MPa, and splitting tensile strength decreases by only 0.2MPa. Building on Bitter and Neilson’s theoretical erosion model, an improved erosion model for BF concrete has been developed under wind gravel flow. This model incorporates the BF mixing factor and particle size function while considering other erosion parameters comprehensively. The enhanced model provides a theoretical foundation and scientific basis for safeguarding concrete structures in Gobi wind-prone regions.

Vitrification-cryopreservation of Shoot Tips Excised from Dormant Bulbs in Fritillaria przewalskii Maxim: Assessing Histological lnjuries and Evaluating Genetic Fidelity in Cryo-Derived Plants

Fritillaria przewalskii Maxim. an essential medicinal plant in the Fritillaria genus of the Liliaceae family, is widely used in traditional medicine. This study focused on developing a cryopreservation protocol for F. przewalskii, an endangered and endemic species in China. We aimed to determine if ex vitro bulbs from field-grown F. przewalskii plants could serve a source of shoot tips for cryopreservation. Key factors influencing cryopreservation outcomes were studied using a vitrification-based approach. Prior to cryopreservation, cold hardening and sucrose preculture conditions were optimized. The best results were achieved by hardening bulbs at 4℃ for 3-4 months,followed by surface disinfection and isolation of 2-5mm Shoot tips. The tips were then then precultured on a medium with 0.5M sucrose for 3 days. Cryopreservation steps included a 20-minute loading treatment, 60-minute PVS2 dehydration at room temperature, and storage in liquid nitrogen more than one hour. After thawing, this protocol resulted in 93% shoot survival rate, with tips regenerating into small bulbs within 5 weeks without intermediary callus formation. Histological analysis showed that while cooling and rewarming caused severe bud damage, apical meristem cells are the primary survival sites. Although loading and PVS2 treatment induced cell plasmolysis, it was not lethal. Optimal PVS2 treatment enhanced cellular dehydration resistance, promoting cell viability at ultralow temperature. RAPD analysis of regenepercentaged plants showed no genetic variation, confirming strong genetic stability. The direct use of ex vitro shoot tips provides a straightforward and effective cryopreservation method for F. przewalskii, combining convenience, efficiency, and genetic stability.

Structural response of precast reinforced concrete structure with emulative moment connections under internal column loss scenarios

Depending on the design, the connections of precast concrete (PC) structures can be susceptible to severe damage under large deformation. Consequently, PC structures may behave differently from cast-in-situ reinforced concrete (RC) structures under column loss scenarios. This study focuses on a class of PC structures that utilize splice sleeves (SSs) to assemble the precast components with integrated beam-column connections (IBCCs). This PC design can alleviate rebar congestion issues and minimize the need for post-cast concrete in the connection regions during assembly. The IBCCs are typically designed to mimic the behavior of corresponding RC connections under service loading conditions. However, the extent of structural emulation under column loss scenarios is not immediately clear. In this paper, the collapse behavior of PC structures with IBCCs under an internal column removal scenario is thoroughly assessed using experimental investigations and numerical analyses. Three PC subassemblages and one RC subassemblage are tested experimentally to provide experimental data for the calibration and validation of finite element (FE) models. The collapse behavior of full-span PC and RC subassemblages are next compared numerically under the internal column loss scenario, with an accompanying investigation on the influence of horizontal SSs. It is found that the PC subassemblages exhibit emulative collapse behavior as the RC subassemblage under internal column loss scenarios, provided that the horizontal SSs are located beyond the beam plastic region under the internal column loss scenario. To achieve the latter, a minimum distance of 0.15 L (L is clear beam span before column removal) is recommended for the positioning of horizontal SSs under the internal column loss scenario. Finally, it is shown that the fracture load and corresponding displacement can be enhanced with the incorporation of high ductility rebars.

Global tsunami modelling on a spherical multiple-cell grid

A model of shallow water equations (SWEs) on a spherical multiple-cell (SMC) grid of 4-level (2.5-5-10-20 km) spatial resolutions is used to simulate tsunami propagation on global ocean surfaces. The unstructured SMC grid retains rectangular cells of the longitude-latitude grid so that efficient finite-difference schemes could be used. It also supports multi-resolutions like mesh refinement to resolve small islands and coastline details while keeping models compact enough to fit into available computers. Two earthquake-induced tsunami cases are simulated and compared with available observations. Results indicate that the modelled tsunami arrival times agree well with observations while tsunami wave heights are underestimated, particularly the observed runups on remote coastal lands. Possible reasons for this underestimation include the smoothing scheme used to suppress numerical oscillations and the missing of initial kinetic energy input from the earthquakes. Another reason is the limitation of the SWEs to describe coastal bores and breaking waves in coastal waters. A possible tsunami scenario induced by a landslide in the Canary Islands is also simulated to assess its potential impact on Atlantic coastal regions. Model results indicate that this kind of tsunami may cause severe damage to local areas but its effects on far fields, like the UK and American coastal regions are small. As the initial landslide disturbance is overly simplified, this study does not give a true representation of a real landslide tsunami but rather a qualitative assessment of its impact on the Atlantic Ocean. More realistic initial condition and improved model representation of coastal processes are needed for further studies of this possible landslide hazard.

Streptococcus equi Subspecies zooepidemicus Endocarditis and Meningitis in a 62-Year-Old Horse Rider Patient: A Case Report and Literature Review

The present article presents a case report and literature review concerning the Streptococcus equi subspecies zooepidemicus (SEZ), a rare zoonotic pathogen in humans. The case involves a 62-year-old man with no prior heart disease, presenting with endocarditis, pneumonia, and meningitis following close contact with a horse. The patient underwent urgent aortic valve replacement due to severe valvular damage caused by the infection. Blood and cerebrospinal fluid cultures confirmed the presence of SEZ, and the patient was treated with a combination of antibiotics, followed by a successful step-down to oral therapy using linezolid. A review of 25 additional Streptococcus equi endocarditis cases highlights the rarity of the condition, its association with animal contact, and its tendency to cause multi-site infections, such as pneumonia and meningitis. Early diagnosis, appropriate antibiotic therapy, and, in severe cases, surgical intervention are critical for a favorable outcome. This report emphasizes the importance of recognizing zoonotic infections in at-risk populations and the potential need for public health surveillance in these scenarios.

High-refractive-index glass device fabricated by room temperature bonding

Abstract Wafer bonding is an essential technology for attaching various materials, including glass. Glass-glass bonding has been applied to fabricate glass micro/nanofluidic devices that work as analytical tools for chemical and biological research. Here, we report the fabrication of micro/nanofluidic devices consisting of a high refractive index (HRI) glass as a component of the device for the first time. Although HRI glass has been used for high-sensitivity and high-resolution imaging in microscopy, it has not been used for glass fluidic devices due to the lack of bonding method for HRI glass. The glass fluidic devices were made through glass-glass bonding at room temperature, which could avoid severe damage to bonded substrates of two glass types due to different rates of thermal expansion. The pressure endurance of these devices was measured to find the correlation with the surface properties of glass substrates. The measured results indicated that chemical groups other than SiOH enhanced the pressure endurance of the devices. Thus, these chemical groups helped to form the fluidic devices consisting of HRI glass and another glass type, borosilicate or fused silica, to achieve practical pressure endurance up to 270 kPa. The HRI glass-incorporating device showed 8-fold higher sensitivity and 30 % narrower spatial resolution of fluorescence signals than the other devices with non-HRI glass.

Progress in Transdifferentiation of Autologous Alternative Cell Sources into Corneal Epithelial Cells.

Corneal limbal epithelial stem cells (LESCs) play a crucial role in corneal epithelium regeneration. Severe damage to these cells can result in limbal stem cell deficiency (LSCD), characterized by repeated corneal conjunctivalization, leading to corneal turbidity and scar formation. Restoring functional LESCs and their ecological location are essential for treating LSCD. The goal of this review is to provide researchers and clinicians with key insights into LESCs biology and to conclude the current cell-based therapies advancement in LSCD treatments. Therapeutic cell resources mainly include mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), skin keratinocyte stem cells (SKCs), and oral mucosal epithelial cells (OMECs).

Causes and Impacts of Flood Events in Emilia-Romagna (Italy) in May 2023

On 1–3 May 2023, severe hydro-meteorological events occurred in the Italian Emilia-Romagna region. Such events caused extensive flooding, landslides, isolation of many areas, evacuation of many families, and severe damage to infrastructure, agriculture, buildings, and essential services. Several municipalities were affected, thousands of civilians had to be evacuated, and losses of life occurred. The consequences beyond the recorded immediate impacts on infrastructure and life were impressive, and extended to the regional economy, specifically in the Fruit Valley, where, in addition to immediate yield losses, long-term damage to orchard production is expected due to persistent flooding. The civil and cultural building heritage has also been heavily affected, both in the countryside and in inhabited centers. Some of the damage, direct and indirect, caused by flooding on buildings will also see an evolution in the medium- to long-term that needs to be addressed. This paper analyzes the manifold aspects of such an atmospheric phenomenon and its impacts to understand the potential increasing occurrence of similar events in the climate change context.

Squamous Cell Carcinoma: From Diagnosis to Effective Treatment-A Comprehensive Guide

Squamous cell carcinoma (SCC) is the second most common malignant skin cancer, and its occurrence is on the rise globally. The main risk factors are cumulative sun exposure, immunosuppression, and inflammation. Early diagnosis and treatment are crucial to prevent metastasis and improve patient outcomes. Advanced diagnostic techniques, such as dermoscopy and reflectance confocal microscopy (RCM), have improved the accuracy of SCC diagnosis. Dermoscopy allows for better identification and targeted biopsy of suspicious areas, while RCM provides noninvasive and precise monitoring of treatment progress. The treatment of SCC has also advanced significantly in recent years. For patients with severe actinic damage and multiple in situ/low-risk SCC, cancer treatment has shown promising results. This approach involves the use of topical agents to target the tumor and the surrounding areas where cancer may develop. Additionally, novel therapies, such as epidermal growth factor receptor inhibitors and immune checkpoint inhibitors have been developed for locally advanced and metastatic SCC. In conclusion, the incidence of SCC has been increasing globally, and early diagnosis and treatment are crucial to prevent metastasis and improve patient outcomes. Advanced diagnostic techn

Role of Hypoperfusion Intensity Ratio in Vessel Occlusions: A Review on Safety and Clinical Outcomes.

The hypoperfusion intensity ratio (HIR) is a quantitative metric used in vascular occlusion imaging to evaluate the extent of brain tissue at risk due to hypoperfusion. Defined as the ratio of tissue volume with a time-to-maximum (Tmax) of >10 seconds to that of >6 seconds, HIR assists in differentiating between the salvageable penumbra and the irreversibly injured core infarct. This review explores the role of HIR in assessing clinical outcomes and guiding treatment strategies, including mechanical thrombectomy and thrombolytic therapy, for patients with large vessel occlusions (LVOs). Evidence suggests that higher HIR values are associated with worse clinical outcomes, indicating more severe tissue damage and reduced potential for salvage through reperfusion. Additionally, HIR demonstrates predictive accuracy regarding infarct growth, collateral flow, and the risk of reperfusion hemorrhage. It has shown superiority over traditional metrics, such as core infarct volume, in predicting functional outcomes. HIR offers valuable insights for risk stratification and treatment planning in patients with LVOs and distal medium vessel occlusions (DMVOs). Incorporating HIR into clinical practice enhances patient care by improving decision-making processes, promoting timely interventions, and optimizing post-intervention management to minimize complications and improve recovery outcomes.

Primary metabolic profiling of four broomrapes belonging to Orobanche and Phelipanche species

Abstract Genera of the Orobanchaceae family are holoparasites that parasitize various hosts. Several members of this family cause severe damage to diverse crop plants. While the biological and life cycles of these parasites have been studied, their metabolism has received little attention, most of which focused on Phelipanche aegyptiaca. This study aimed at obtaining more knowledge about the primary metabolic profiling of four parasite species belonging to the Orobanchaceae family – Orobanche cumana, Orobanche cernua, Phelipanche aegyptiaca and Phelipanche ramosa – that developed on tomato (Solanum lycopersicum L.) as a host. Using GC-MS, it was shown that significant differences in metabolites content occur between species belonging to Orobanche compared to those belonging to Phelipanche. This finding adds another layer to the separation of these two genera in addition to morphological separation. Moreover, each of these four species exhibits different metabolic profiles, indicating that the parasites can absorb the host’s metabolites but also have the ability to self-regulate their metabolites in order to grow and develop.

A multitask SHM algorithm to identify damage with random severity and location in IPE beams using EMI technique

Existing electromechanical impedance (EMI) damage identification algorithms often face challenges in terms of generalizability. This paper presents a robust algorithm that can simultaneously estimate the region and severity of damage with random damage scenarios across a surface and any severity, rather than being limited to specific points and severities. The host structure was an I-beam. Simulated damage was introduced as a subtle added mass to evaluate the algorithm's effectiveness in early-stage damage identification. Initially, various EMI tests with different damage specifications were conducted, and validated through numerical simulation. Damage-sensitive features were extracted and were input into three ML models: support vector machine, random forest, and multilayer perceptron. An ensemble learning approach was employed to combine the individual predictions from these models. The algorithm achieved classification accuracies of 97.3 % and 94.4 % on the validation and test sets, respectively, for identifying damaged regions. The algorithm also quantifies damage severity, achieving R-squared values of 92 % and 88 % on the validation and test sets, respectively.