Serum Omentin-1 Research Articles

Assessment of Serum Concentrations of Omentin-1 in Children with Type 1 Diabetes as Indicator of Insulin Resistance

Background: Omentin-1, a protein produced mainly in visceral adipose tissue. Its function is most likely to increase insulin sensitivity and stimulate glucose metabolism. This effect can be both local and systemic. Objective: To evaluate the concentrations of selected gastric peptide omentin-1 in serum of children with type 1 diabetes, relevant to the disease duration. Patients and Methods: This case control prospective study was conducted during the period from 2018 to 2020. This study was carried out in Endocrinology Unit and Outpatient Clinic at Pediatric Department, Zagazig University Hospital. The sample size was 85 (17 in every group). Serum omentin-1 level was measured. Results: Cases were significantly higher than control regarding FBS, PPBS and HA1c. Cases were significantly higher than control as it was distributed as 426.04 ± 137.6 and 103.21 ± 32.5 respectively. Significant AUC with cutoff > 191.7 and 100.0% sensitivity and specificity. Regarding FBS & PPBS, control group were significantly lower than other groups but group 4 was significantly higher than other groups regarding PPBS. Regarding HA1c, control group was significantly lower than other groups but groups 1 & 4 were significantly higher than other groups. Concerning omentin, control group was significantly lower than other groups and group 4 was significantly higher than other groups also groups 2 & 3 were significantly higher than group 1. Conclusion: T1DM is a common health problem in the pediatric age group. Omentin-1 level was significantly high in T1DM and its level is strongly correlated to duration and insulin resistance.

Serum omentin-1 levels in hypertensive patients.

Hypertension (HT) is a disease that can cause death due to multiple target organ damage and eventually related vascular system damage. High blood pressure is known increased inflammatory activity and to cause endothelial dysfunction has been showed in HT patients. Omentin-1 is a glucoprotein of the adiponectin family released from visceral adipose tissue, endothelial cells, and visceral fat stromal-vascular cells. It has anti-inflammatory effect and circulating omentin-1 concentration correlates negatively with waist circumference, insulin resistance, and body-mass index. Serum omentin-1 is used as a biomarker of coronary artery disease, obesity, cancer, metabolic syndrome, inflammatorydisease, atherosclerosis, and diabetes mellitus. The aim of our study is to investigate circulating omentin-1 levels in HT patients compared to healthy normotensive controls. Patients diagnosed with new essential HT (n = 61) and healthy normotensive individuals (n = 60) were enrolled in this study. The HT group was separated into two subgroups. There were 30 patients in stage 2 HT group and 31 patients in stage 1 HT group. Omentin-1 levels were significantly lower both in stage 1 and 2 HT subgroup as compared with the normotensive controls (72.19 ± 54.33 ng/ml for stage 1 HT subgroup; 62.45 ± 47.01 ng/ml for stage 2 HT subgroup; and, 147.84 ± 58.55 ng/ml for healthy normotensive controls; overall P < 0.001). The present study demonstrated that serum Omentin-1 levels decreased in patients with HT compared with normotensive controls. These lower concentrations may be attributed to a combined outcome of endothelial dysfunction, renal injury, and inflammation in the setting of hypertension.

Association of Serum Vitamin-D and Omentin-1 Levels in Post- Menopausal Female with Coronary Artery Disease

Objective: The objective of the present study was to investigate the association between serum vitamin- D (calcidiol, D2) and omentin-1 levels in pre- and post-menopausal female patients with coronary artery disease (CAD).Methods: This cross-sectional, case-based study was conducted in the cardiac ward of Civil Hospital Karachi from July 2016 to June 2017. Total 110 diagnosed female patients of coronary artery disease were included randomly in the study, out of which, 42 women were pre-menopausal, whereas, 68 post-menopausal. The diagnosis was based on coronary angiography. Serum Vitamin-D and omentin-1 levels were determined by using enzyme-linked immunosorbent assay (ELISA) in Dr Abdul Qadeer Khan Institute of Biotechnology and Genetic Engineering (KIBGE). Serum vitamin-D concentrations were classed as sufficient ( 30 ng/mL); deficient (10 to&lt; 29 ng/mL); and insufficient ( &lt;10 ng/mL). Data were analyzed by SSPS version 16.Results: From our study, we observed significant low levels of serum vitamin-D and omentin-1 in pre and post-menopausal females of coronary artery disease, however, severe deficiency of Vitamin-D (&lt;10 ng/L) was more associated with post-menopausal females. Vitamin-D deficiency (&lt;30ng/L) was found in 82.72% (n=96) of CAD females, moreover; 46.36% (n=51) patients were found with severe vitamin-D deficiency placed in group I, 40.90% (n=45) patients were found with a moderate deficiency (17.09 ± 4 ng/mL) in group II, whereas only 12.72% (n=14) had optimal serum vitamin-D levels placed in group III. Serum vitamin-D (calcidiol, D2) level was associated positively with omentin-1 in CAD patients after adjustment for potential confounding variables; basal metabolic rate, waist circumference, blood pressure and lipid profile in multivariable linear regression analysis.Conclusion: Within the limits of the study, we concluded that low levels of vitamin-D and omentin-1 are associated with both pre and post-menopausal females with prevalent coronary artery disease (CAD). Further investigations are required in different ethnic groups and populations to confirm the findings.

Role of swimming on muscle PGC-1\u03b1, FNDC5 mRNA, and assessment of serum omentin, adropin, and irisin in high carbohydrate high fat (HCHF) diet induced obesity in rats

BackgroundExercise benefits a variety of organ systems in mammals, and some of the best recognized effects of exercise on muscle are mediated by the transcriptional peroxisome proliferator-activated receptor gamma co-activator 1-α (PGC-1α). The regulatory effect of swimming on muscle PGC-1α, FNDC5 mRNA expression, and subsequently irisin levels is more controversial. This study aimed to investigate the role of swimming as an exercise on the expression of peroxisome proliferator-activated receptor-gamma coactivator1 alpha (PGC-1α) and Fibronectin type III domain containing 5 (FNDC5) mRNA in skeletal muscle and assessment of serum omentin, adropin, irisin, and PGC-1α levels in high carbohydrate high fat (HCHF) diet induced obesity in rats. Sixty male albino rats are randomly divided into 4 groups (15 rats/group). In the first group (control), rats are fed with standard diet. The 2nd group (cont + swim) is fed on standard diet and made swimming exercise. The 3rd group of rats is fed on HCHF, whereas in the 4th group (HCHF + swim) is also fed on HCHF diet and made swimming exercise for 20 weeks. Blood glucose, insulin, HOMA-IR, lipid profile, omentin, irisin, adropin, and PGC-1α were measured. Also, FNDC5 and PGC-1α are extracted and purified from muscle tissue samples measured by PCR test.ResultsOur results showed significant increase in glucose, insulin, insulin resistance, cholesterol, and triglycerides with significant decrease in omentin, irisin, adropin, PGC-1α, and HDL in HCHF group as compared to the control group. These results improved after exercise in all parameter in HCHF + swim group compare to HCHF group. Also, there was inverse correlation between omentin and fasting glucose and HOMA-IR in HCHF + swim group.ConclusionsIt concluded that swimming exercise improved all the above measured parameters in serum and tissues which might have been promising for the prevention of metabolic diseases.

Association between Omentin-1 and Coronary Artery Disease: Pathogenesis and Clinical Research.

Like other adipokines, omentin-1 is secreted from visceral adipose tissue and plays a vital role in the development of chronic inflammatory diseases, including cardiovascular events. Recent studies have shown that circulating omentin-1 levels are associated with various metabolic risk factors, such as high blood pressure, increased waist circumference, dyslipidemia, and glucose intolerance. The decrease in serum omentin level is an independent predictor of Coronary Artery Disease (CAD) and is associated with the severity of this disease. Since there is no relevant review in the literature, we aimed to summarize the studies on the relationship between omentin-1 and CAD.

Role of Omentin-1 in the Diagnosis and Prognosis of Breast Cancer in Egyptian Females.

Background: The most common malignancy of females of Egypt is breast cancer. It accounts for 32 per cent of women's cancer. Clarifying and assessing prognostic factors associated with breast cancer is essential as they are the corner stone to identify the high-risk groups that can benefit from adjuvant therapy and improve their prognosis for the disease. Study Objective: The aim of this study was to assess the role of serum omentin-1 levels in the Egyptian female population as prognostic markers for breast cancer. Patients and methods: Study subjects are 120 BC patients and 30 age and BMI matching health controls, serum omentin-1 levels have been investigated and correlated with the clinicopathological aspects of the BC patients Results : Our study revealed a significant difference in omentin-1 between healthy controls and BC patients, and a gradual increase in severity of the disease. Conclusion: our findings suggested that omentin-1 could be a diagnostic marker for early detection of cases and associated with the prognosis of breast cancer, supporting its role as biomarkers of the prognosis.

The association between serum omentin level and bodyweight: A systematic review and meta-analysis of observational studies.

A number of studies have examined the association between omentin and body weight, but the findings have been inconclusive. Here in, we systematically reviewed available observational studies to elucidate the overall relationship between omentin and body weight, by comparison of serum omentin level in overweight/obese and normal weight subjects. PubMed, Science direct, Scopus and ISI web of science databases were searched for all available literature until January 2020 for studies assessing the association between omentin and body weight. The Newcastle-Ottawa Quality Assessment Scale was used to assess the quality of each study. A total of 27 articles met the inclusion criteria and were included in our systematic review and meta-analysis. There was a significant association between omentin serum level and body weight (Standard Mean Difference (SMD)-0.43; 95% CI,-0.70 to-0.15; P=0.002; I2=93.2%). In order to find the probable source of heterogeneity subgroup analysis based on the participants' age (adolescent, adult), gender (male, female, both gender), health status (healthy, unhealthy), geographical location (Asian, non-Asian countries), study quality (low, medium, high), study design (case-control, cross-sectional), participants' health status (healthy, unhealthy) and BMI (obese, overweight) was carried out. According to what was discussed, we found that serum omentin level is significantly lower in overweight subjects but not obese ones. This finding should be interpreted cautiously because of significant heterogeneity among included studies.

Is there a relationship between serum omentin level and acute phase response in patients with familial Mediterranean fever?

Familial Mediterranean fever (FMF) is an autoinflammatory disease characterized by frequent attacks and chronic inflammation. Subclinical inflammation continues during the attack-free period. Omentin is an anti-inflammatory adipokine, which plays important roles in the adjustments of glucose metabolism, cardiovascular homeostasis and atherosclerosis. The aim is to investigate the omentin levels in FMF patients and to assess the association with markers of subclinical inflammation in FMF patients such as serum amyloid A (SAA), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). This cross-sectional study included 54 consecutive adult FMF patients (27 male, 27 female) and 28 healthy individuals (16 male, 12 female). The FMF patients were separated into 3 groups: (1) attack-free group, (2) active-attack group and (3) colchicine-resistant group. Serum omentin levels were compared between the FMF patients and the healthy control group. A significant difference was determined between the FMF patients and healthy control subjects in terms of omentin levels (108.05 (19.97-343.22) vs. 199.5 (42.98-339.41) p < 0.05). SAA values were significantly higher in the FMF patients compared with the healthy control group. When the FMF patients were examined as separate groups, serum omentin values were lower in the colchicine-resistant group than in the groups without resistance (76.64 (19.77-224.33) vs. 186.47 (28.41-343.21) p = 0.006). FMF patients with colchicine resistance are associated with decreased omentin concentrations, probably mediated by inflammation-driven mechanisms. Key Points • Omentin is a type of adipokine which has an anti-inflammatory effect by inhibiting the inflammatory cytokine network. • Decreased omentin levels are associated with increased obesity, insulin resistance and comorbidities. • We report that omentin levels fluctuate in various diseases. In addition, we have focused on the levels of omentin in patients with FMF, as it may act as a biomarker for colchicine resistance.

Comparion Of Omentin-1 And Insulin Resistance Markers In Different Physical Activity Levels Older Adults

PURPOSE: Omentin-1 is a good adipokine produced by omental adipose tissue and plays a pivotal role in regulating the insulin resistance. The aim of this study was to compare serum omentin-1 concentration together with insulin resistance markers in active-obese, inactive-obese, normal-weight Chinese older adults. METHODS: 128 older adults (men: n=32, women: n=96) were recruited to participate in this study. Subjects were divided into the following three groups. 1) active-obese (n=49, age=63±6years; BMI=25.9±2.3kg/m2); 2) inactive-obese (n=48, age=66±6years; BMI=26.5±2.4kg/m2); 3) normal-weight (n=31, age =64 ±5years; BMI=21.1±1.5kg/m2). ActiGraph GT3X-BT triaxial accelerometers were used to measure the levels of physical activity. We define it as active subjects based on average number of minutes spent on the moderate-to-vigorous physical activity (180min/week). Venous blood samples were collected to measure omentin-1, insulin and glucose levels. The homeostasis model assessment of the insulin resistance (HOMA-IR) was calculated as the following formula: fasting glucose (mmol/l) × fasting insulin (mU/l)/22.5. RESULTS: The results showed that omentin-1 concentration was significantly lower in obese than normal-weight group (17.1±12.4 vs. 24.2±13.4ng/ml, p<0.01). Meanwhile, Glucose, insulin and HOMA-IR was significantly higher in obese than in normal-weight group (5.3±1.2 vs. 4.8±0.4mmol/L, 7.9±3.8 vs. 4.7±1.9μU/mL, 1.94±1.18 vs. 1.00±0.39, both p<0.05). However, there was no significant difference between serum omentin-1 levels and glucose in active-obese and inactive-obese groups (both p>0.05). Insulin and HOMA-IR in active-obese were significantly lower than inactive-obese group (7.2±3.1 vs. 8.8±4.3μU/mL, 1.68±0.90 vs. 2.22±1.41, both p<0.05). CONCLUSIONS: Our results suggested that physical activity can improve the negative effects of obesity on insulin resistance markers, but did not affect the concentration of omentin-1 significantly of obese older adults. ACKNOWLEDGMENTS: This study was supported by Ministry of Science and Technology of the People's Republic of China (Grants No.2013FY114700).

Circulating omentin-1 levels and inflammation in polycystic ovary syndrome.

The aim of the study was to analyze interrelation between plasma omentin-1 levels and nutritional status and inflammation in PCOS. A cross-sectional study involving 86 PCOS (47 obese) and 72 Non-PCOS women (41 obese) determined anthropometric parameters and body composition. Serum glucose, insulin and omentin-1, TNF-α, sTNFRs, IL-6 and sR-IL6 were measured in the fasting state. Plasma omentin-1 levels were significantly lower in the PCOS than in the Non-PCOS group and both corresponding normal weight and obese subgroups. In three analyzed least-angle regression (LARS) models the lower plasma omentin- 1 levels was associated with PCOS occurrence, higher circulating TNF-α and lower IL-6 levels. Suppressed omentin-1 levels in PCOS are characteristic for this disturbance and proinflammatory cytokines are factors modifying secretion of this adipokine.

Omentin-1 attenuates adipose tissue inflammation via restoration of TXNIP/NLRP3 signaling in high-fat diet-induced obese mice.

Omentin-1 is an adipokine expressed by the adipose tissue and is reduced in obesity. This study was designed to calculate the protective efficiency and mechanism of omentin-1 against inflammation of the adipose tissue in obese mice. A transgenic mouse model with omentin-1 protein overexpression was established by crossing omentin-1 transgenic mice with Fabp4-Cre mice. Obesity was induced in the mice by feeding them a high-fat diet for 10weeks. Fabp4-Cre-mediated overexpression of omentin-1 significantly increased serum omentin-1 level, serum anti-inflammatory factor levels, and expression of M2-specific mRNAs; inhibited body weight and adipose tissue weight gain; improved glucose tolerance, insulin tolerance, and insulin sensitivity; decreased serum levels of insulin and proinflammatory factors, adipocyte size, and expression of M1-specific mRNAs; suppressed macrophage infiltration; downregulated expression of proinflammatory factors; upregulated expression of anti-inflammatory factors; and inhibited thioredoxin-interacting protein (TXNIP)/NOD-like receptor 3 (NLRP3) signaling in the adipose tissue of obese mice. An NLRP3 inhibitor (20mg/kg MCC950) exhibited the same effects as overexpression of omentin-1. Pretreatment with omentin-1 inhibited lipopolysaccharide-induced inflammation via TXNIP/NLRP3 signaling in RAW 264.7 macrophages. These findings suggest that omentin-1 suppresses adipose tissue inflammation in obese mice, at least partly, via inhibiting the TXNIP/NLRP3 signaling pathway.

Omentin: a biomarker of cardiovascular risk in individuals with axial spondyloarthritis

Cardiovascular (CV) disease is the main cause of mortality in axial spondyloarthritis (axSpA). CV risk is enhanced by dysregulation of adipokines. Low omentin levels were associated with metabolic dysfunction and CV disease in conditions different from axSpA. Accordingly, we evaluated the genetic and functional implication of omentin in CV risk and subclinical atherosclerosis in a cohort of 385 axSpA patients. Subclinical atherosclerosis was evaluated by carotid ultrasound. Omentin rs12409609, in linkage disequilibrium with a polymorphism associated with CV risk, was genotyped in 385 patients and 84 controls. Serum omentin levels were also determined. omentin mRNA expression was assessed in a subgroup of individuals. Serum and mRNA omentin levels were lower in axSpA compared to controls. Low serum omentin levels were related to male sex, obesity, inflammatory bowel disease (IBD) and high atherogenic index. rs12409609 minor allele was associated with low omentin mRNA expression in axSpA. No association was observed with subclinical atherosclerosis at the genetic or functional level. In conclusion, in our study low omentin serum levels were associated with CV risk factors in axSpA. Furthermore, rs12409609 minor allele may be downregulating the expression of omentin. These data support a role of omentin as a CV risk biomarker in axSpA.

Association of serum Vitamin D and omentin-1 in patients with coronary artery disease.

Objectives: The aim of the present study was to investigate the association between serum vitamin D (calcediol, D2) with omentin-1 in patients with coronary artery disease (CAD). Study Design: Case Control Study/ Cross Sectional Study. Setting: Civil Hospital Karachi (CHK). Period: January 2016 to June 2016. Material &amp; Methods: In this cross-sectional study, total of 250 cases of coronary artery disease were recruited randomly from the civil hospital, Karachi. Diagnosis based upon coronary angiography. Serum Vitamin D and omentin levels determined by using enzyme linked immunosorbent essay (ELISA) in Dr. Abdul Qadeer khan Institute of Biotechnology and Genetic Engineering (KIBGE). 120 apparently healthy controls were recruited. Results: we observed significant low levels of serum vitamin D and omentin-1in patients of coronary artery disease. Vitamin D (calcidiole, D2) deficiency (&lt;30ng/L) was found in 82 % (n= 205) of CAD patients, moreover; 50.8% (n=127) patients were found with severe vitamin D deficiency (8.77± 3.87) placed in group III, 31.2% (n=78) patients were found with moderate deficiency (17.09 ±4 ng/mL) in group II, whereas only 18% (n=45) had optimal serum vitamin D levels (33.02 ±16.00 ng/ mL) placed in group I. Mean serum omentin-1 level was (578± 21.87, 409±32.09, 321± 23.01) in these subgroups respectively. Serum vitamin D (calcidiole, D2) level was associated positively with omentin-1 in CAD patients (P = 0.002) after adjustment for potential confounding variables; basal metabolic rate, waist circumference, blood pressure and lipid profile. Conclusion: Within the limits of the study, we concluded that low levels of vitamin D and omentin-1 are associated with prevalent coronary artery disease (CAD) and are independent of other cardiovascular risk factors. Further investigations are required in different ethnic groups and populations to confirm the findings.

Clinical relevance of serum omentin-1 levels as a biomarker of prognosis in patients with acute cerebral infarction.

Background and purposePrevious studies have shown that adipocytokines are associated with atherosclerosis, diagnosis, and functional prognosis after ischemic stroke. However, few studies have investigated the relationship between omentin‐1 and atherosclerotic acute cerebral infarction (ACI).MethodsIn this study, we investigated the association between serum omentin‐1 levels at admission and severity, infarction volume, and functional prognosis of patients 90 days after atherosclerotic ACI.ResultsA total of 109 patients with atherosclerotic ACI were enrolled. Serum omentin‐1 levels at admission were lower in patients with ACI than those in healthy controls (47.18 ± 13.64 vs. 56.27 ± 34.44 ng/ml, p = .014). Serum omentin‐1 levels at admission were negatively correlated with severity of ACI (r = −.271, p = .004) and infarction volume (r = −.264, p = .006), respectively. Moreover, serum omentin‐1 levels were lower in the poor functional prognosis group than those in the good functional prognosis group in patients with large artery and small artery atherosclerotic ACI. In a logistic regression analysis, higher serum omentin‐1 level (>43.10 ng/ml) at admission was negatively associated with a poor functional prognosis 90 days after atherosclerotic ACI.ConclusionsSerum omentin‐1 levels at admission were significantly lower among patients with ACI. A higher plasma omentin‐1 level (>43.10 ng/ml) was negatively associated with poor functional prognosis 90 days after atherosclerotic ACI. Further studies are needed to investigate the pathophysiological mechanism of omentin‐1 in affecting attacks and prognosis of ACI as well as to confirm the value of plasma omentin‐1 level as a potential biomarker.

Адипокины жировой ткани в патогенезе ожирения и ишемической болезни сердца

Дисбаланс в секреции адипокинов жировой тканью при ожирении может играть роль в развитии сердечно-сосудистых заболеваний. Нами было проведено исследование уровня экспрессии генов адипонектина и оментина-1 в жировой ткани у лиц с ожирением и ишемической болезнью сердца (ИБС). Показано, что сниженные концентрация в сыворотке крови и экспрессия гена адипонектина в подкожной жировой ткани могут вносить вклад в развитие ИБС при ожирении. ИБС ассоциирована с низкой концентрация оментина-1 в сыворотке крови. Imbalance in the secretion of adipose tissue adipokines may play a role in the development of cardiovascular diseases associated with obesity. Investigation of adiponectin and omentin-1 genes expression levels in adipose tissue was conducted in patients with obesity and coronary artery disease (CAD). Our study has shown that reduced adiponectin serum concentration and subcutaneous adipose tissue gene expression may contribute to CAD development. CAD is associated with a low serum omentin-1 concentration.

Association of serum omentin concentration with anthropometric, physiological, and biochemical parameters in obese individuals.

Omentin is a secretory protein produced in visceral adipose tissue. The potential protective action of omentin in metabolic disorders is not yet fully understood. The aim of this study was to determine the association of omentin with anthropometric, physiologic, and biochemical parameters in obese individuals. Sixty obese individuals and 40 normal weight controls were enrolled in the study. Anthropometric measurements were taken, systolic (SBP) and diastolic blood pressures (DBP) were recorded. Serum fasting glucose, insulin, homeostatic model assessment of insulin resistance (HOMA-IR), lipid profile, and serum omentin concentrations were determined. The concentration of omentin in obese individuals was significantly lower than in controls (145.5 ± 33.3 versus 383.6 ± 92.9; P < 0.001). Omentin correlated negatively with waist circumference (P < 0.01), hip circumference (P < 0.05), percent of adipose tissue (P < 0.001), fasting insulin (P < 0.001), HOMA-IR (P < 0.001), and SBP (P < 0.001) in the patients who were obese. A positive relationship was documented with high-density lipoprotein in both the obese patients (P < 0.01) and the overall population (P < 0.001). Multiple negative omentin correlations with body weight, body mass index, waist and hip circumference and its ratio, percent of adipose tissue, SBP, triacylglycerols, glucose, fasting insulin, and HOMA-IR were demonstrated in the entire population. In the multivariate linear regression model, insulin concentration (β=-0.49; P < 0.001), percent of adipose tissue (β=-0.31; P < 0.001), and waist circumference (β=-0.21; P < 0.01) were independent predictors of omentin concentration in individuals who were obese. A concentration of omentin is associated with cardiometabolic risk-related factors in obesity. Fasting insulin, adipose tissue percentage, and waist circumference can be considered candidates for markers of omentin concentration in obese individuals.

MON-691 Association of Serum Level of Omentin-1, Hs-CRP and Atherosclerosis in Patients with Type 2 Diabetes

Omentin-1 has been identified as an interesting novel adipokine that may modulate the action of insulin. Serum omentin-1 levels were associated with endothelial dysfunction and cardiovascular diseases, which highly contribute to morbidity and mortality of patients with diabetes, however its exact biological function is still unclear. The aim of this study was to assess the level of circulating plasma Omentin-1 level in patients with type 2 diabetes compared to control subjects, and to investigate the relationship of circulating Omentin-1levels with HOMA, BMI, lipid profile, epicardial fat, visceral fat and subcutaneous fat in early stages of atherosclerosis measured by carotid intima media thickness (CIMT). This is a cross-sectional observational study which included 45 patients with type 2 DM (not previously diagnosed as ACVD patients) and 30 (age and sex)-matched healthy control subjects. Patients with a history of recent acute infection, malignancy, metabolic or endocrinal diseases, cardiac failure, renal and hepatic impairment were excluded. Participants were subjected to measurement of FPG, HbA1c, lipid profile, calculation of HOMA2, hs CRP, serum omentin-1, CIMT, estimation of visceral, epicardial and subcutaneous fat through CAT scanning. There was no statistically significant difference between both groups in the mean circulating omentin-1 level, a mean of (793.86 ± 195.81) ng/ml in type 2 diabetes Vs (804.93 ± 205.76) ng/ml in the control group,(p=0.654). However, there was a highly statistically significant difference in hs-CRP between both groups, mean hs CRP is (4.29 ± 4.87 mg/l) vs (0.92 ± 0.53 mg/l) respectively. There was a statistically significant negative correlation between omentin-1 and hs CRP (r= -0.278, p= 0.016).There were no statistically significant correlations between omentin-1 with BMI, HbA1c, HOMA, lipid profile or adiposity. These findings may denote the anti-inflammatory effects of omentin-1 which maybe unrelated to insulin resistance and dyslipidemia. Further studies are required to confirm the cardioprotective role of omentin-1 in patients with type 2 diabetes, focusing on its local anti-inflammatory role.Nothing to disclose: MA, NA, NKNo Sources of Research Support

Serum omentin-1 level in patients with benign prostatic hyperplasia

BackgroudTo evaluate the relationship between omentin-1 and benign prostatic hyperplasia (BPH). BPH is the most common urological disease in elderly men worldwide. Lower serum omentin-1 levels were reported to be negatively associated with the incidence of inflammation, diabetes, obesity and metabolic syndrome, which all play a role in the development of BPH. To the best of our knowledge, the relationship between omentin-1 and BPH has not been investigated previously.MethodsA total of 70 males participated in this study, including forty patients diagnosed with BPH and thirty healthy males. The anthropometric measurements and the biochemical parameters were measured in this study. We evaluated serum omentin-1 levels and the correlation with those data. We also test the gene expression of IL-8, IL-18 in BPH group using the TURP tissues.ResultsThe serum omentin-1 levels were lower in the BPH patients than in the control group (27.95 ± 4.18 versus 32.03 ± 5.46, p < 0.001). The general characteristics and biochemical parameters were investigated, and a negative correlation was found between serum omentin-1 levels and BMI in the BPH group (r = − 0.391, p = 0.013) as well as the whole group (r = − 0.457, p < 0.001). Multiple-factor binary regression analysis revealed that serum omentin-1was a protective factor of BPH development. Furthermore, lower serum omentin-1 levels were associated with higher mRNA expression of IL-8 or IL-18 in the BPH group.ConclusionOmentin-1 may suppress the development of BPH and Lower serum omentin-1 levels in BPH patients might associated with higher prostate volume and higher IL-8 and IL-18 expression levels in their prostatic cells.

Ibuprofen increases the serum Omentin levels in rats after abdominal surgery.

AIMS Omentin is an adipokine primarily produced by visceral adipose tissue and its reduced levels have been shown to be associate with worse metabolic outcomes. We aimed to study the effects of preoperative ibuprofen on postoperative omentin levels in rats after surgery. METHODS Forty-eight albino Wistar rats, 6 in each of 8 groups according to the surgical procedure (laparotomy, laparotomy plus ibuprofen (IBU), nephrectomy, nephrectomy plus IBU, hepatectomy, hepatectomy plus IBU, splenectomy and splenectomy plus IBU). The Omentin levels of the groups were postoperatively analyzed. RESULTS The mean omentin was significantly higher in the laparotomy plus IBU group compared to the laparotomy group (p<0.001). Mean Omentin was significantly higher in the hepatectomy plus IBU group compared to the hepatectomy group (p=0.01). Mean Omentin was significantly higher in the nephrectomy plus IBU group compared to the nephrectomy group (p=0.001). CONCLUSION We suggest that preoperative ibuprofen may enhance circulating levels of Omentin, which has beneficial effects in trauma and inflammation settings in subjects that undergo minor or major abdominal surgery.

Evaluation of adipokines, adiponectin, visfatin, and omentin, in uncomplicated type I diabetes patients before and after treatment of diabetic ketoacidosis.

Diabetic ketoacidosis (DKA) is potentially a lethal complication of uncontrolled, especially type 1, diabetes. Understanding the mechanisms underlying adipokine involvement in the regulation of glucose metabolism is important to prevent complications of hyperglycemia. The role of novel adipokines during DKA in human remains unclear. The method is to determine the changes in the circulating levels of adiponectin, visfatin, and omentin after treating DKA in the patients referred to Shohadaye Khalij-e-Fars hospital at the Bushehr University of Medical Sciences. Measuring adipokines (adiponectin, visfatin, and omentin) in 31 patients with DKA who are admitted in Shohadaye Khalij-e-Fars hospital at the Bushehr University of Medical Sciences. Adipokines are measured at the time of admission and after recovery from DKA, using ELISA method. After recovery from DKA, omentin-1 serum concentration decreased significantly (from 183 to 165), but adiponectin and visfatin did not change significantly. Omentin-1 may play a significant role in insulin resistance during the DKA and could be potentially recommended as a marker of recovery in DKA.