Sensitive Fluorescence Turn-on Research Articles

Visual Tracking of Hydrogen Sulfide: Application of a Novel Lysosome-Targeted Fluorescent Probe for Bioimaging and Food Safety Assessment.

The equilibrium state of hydrogen sulfide (H2S), a gaseous signaling molecule produced by lysosomal metabolites, in vivo is crucial for cellular function. Abnormal fluctuations in H2S concentration can interfere with the normal function of lysosomes, which has been closely linked to the pathogenesis of a variety of diseases. In view of this, a novel fluorescent probe Lyso-DPP based on 1,3,5-triarylpyrazolines was developed for the precise detection of H2S in lysosomes by using the hydrophilic morpholine moiety as a lysosomal targeting unit, and 2,4-dinitroanisole as a fluorescence-quenching and H2S-responsive unit. The probe cleverly combines the advantages of simple synthesis, sensitive blue fluorescence turn-on with a limit of detection, LOD, of 97.3 nM, good stability, and fast response time (10 min), which makes Lyso-DPP successful in portable monitoring of meat freshness in the form of test strips. Moreover, the excellent biocompatibility and precise targeting capability of the probe Lyso-DPP make it perform well in the monitoring of H2S in lysosomes, living cells, and zebrafish. This work not only provides new technical tools for food quality control but also paves up new ideas for early diagnosis and treatment of H2S-related diseases.

Highly sensitive and selective fluorescent "turn-on" sensor for Ag+ detection using MAPbBr3@PCN-221(Fe): An efficient Ag+-bridged energy transfer from perovskite to MOF.

Metal ion-induced water pollution is attracting increasing public attention. Perovskite quantum dots and metal-organic frameworks (MOFs), owing to their outstanding properties, hold promise as ideal probes for detecting metal ions. In this study, a composite material, MAPbBr3@PCN-221(Fe), was prepared by encapsulating MAPbBr3 quantum dots with PCN-221(Fe), demonstrating high chemical stability and good reusability. The composite material shows a sensitive fluorescence turn-on signal in the presence of silver ions. The fluorescence intensity of the composite material exhibits a linear relationship with the concentration of Ag+ in the solution, with a low detection limit of 8.68 µM. Moreover, the fluorescence signal exhibits a strong selectivity for Ag+, enabling the detection of Ag+ concentration. This fluorescence turn-on signal originates from the Ag+-bridged energy transfer from the conductive band of MAPbBr3 to the excited state of the MOF, which is directly proportional to the concentration of silver ions. Simultaneously, this finding may open up a new possibility in artificial controlled energy transfer from perovskite to MOF for future development.

A turn-on fluorescent probe based on pyrene for hydrazine detection

This paper presents a novel fluorescent probe (GNEM) based on a pyrene fluorophore, with dicarboximide as the recognition unit for hydrazine. The fluorescence probe can be synthesized in a simple one-step process. A highly selective and sensitive fluorescence turn-on GNEM synthesized. GNEM react with hydrazine through nucleophilic addition, leading to the deprotection of dicarboximide and the release of dicarboximide hydrazine, resulting in significant blue fluorescence. This enhanced emission is attributed to the intramolecular charge transfer (ICT) sensing mechanism of GNEM. In addition, detection of hydrazine in soil and water is conducted. By designing a simple detection device, the hydrazine content in the air can be quantitatively measured.

A phenothiazine coumarin based fluorescent probe with AIE feature for real-time detecting lysosomal ClO– in living cell

A new phenothiazine coumarin based probe WX-Lyso for detection of ClO– was developed. The probe showed obvious aggregation-induced emission (AIE) characteristic and exhibited a highly selective and sensitive turn-on fluorescence signal at 544 nm for ClO– with a large Stokes shift of 144 nm. The limit of detection (LOD) of ClO– was measured as low as 0.99 μM and a good line equation was obtained in the 0–50 μM concentration range. The detection mechanism was elucidated FT-IR, ESI-MS analysis and DFT. Furthermore, the probe had been demonstrated successfully has good lysosomal-targeting and high-contrast imaging abilities, which has the potential to become a new chemical tool and provide help for detecting ClO– in living systems.

An environmentally sensitive zinc-selective two-photon NIR fluorescent turn-on probe and zinc sensing in stroke

A two-photon near infrared (NIR) fluorescence turn-on sensor with high selectivity and sensitivity for Zn2+ detection has been developed. This sensor exhibits a large Stokes' shift (∼300 nm) and can be excited from 900 to 1000 nm, with an emission wavelength of ∼785 nm, making it ideal for imaging in biological tissues. The sensor's high selectivity for Zn2+ over other structurally similar cations, such as Cd2+, makes it a promising tool for monitoring zinc ion levels in biological systems. Given the high concentration of zinc in thrombi, this sensor could provide a useful tool for in vivo thrombus imaging.

Fluorescence switch-on detection of alkaline phosphatase activity and adenosine triphosphate using vitamin B6 cofactor conjugated Mn-doped ZnS QDs

This study introduces a fluorescent nanoprobe PLP@l-Cyst-Mn-ZnS obtained through post-functionalization of l-cysteine capped Mn-doped ZnS quantum dots (l-Cyst-Mn-ZnS QDs) with pyridoxal 5′-phosphate (PLP). The functionalization process involves the formation of a Schiff base between PLP and l-Cyst, leading to the quenching of fluorescence emission at 603 nm. The quenched PLP@l-Cyst-Mn-ZnS acts as a highly sensitive fluorescent turn-on probe for alkaline phosphatase (ALP) and adenosine triphosphate (ATP). ALP catalytically dephosphorylates the functionalized PLP into pyridoxal (PL). In contrast, the interaction of ATP with the functionalized PLP suppresses the electron transfer process leading to the recovery of fluorescence emission at 603 nm. This nanoprobe exhibits exceptionally high sensitivity enabling the estimation of ALP and ATP concentrations as low as 0.006 U/L and 0.14 µM, respectively. The practical applicability of PLP@l-Cyst-Mn-ZnS is demonstrated by accurately quantifying spiked ALP and ATP in human serum and plasma. This work highlights the potential of PLP@l-Cyst-Mn-ZnS as a versatile and efficient nanoprobe for biomedical applications.

A Turn-On Fluorescence Sensor Based on Nitrogen-Doped Carbon Dots and Cu2+ for Sensitively and Selectively Sensing Glyphosate

Glyphosate has excellent herbicidal activity, and its extensive use may induce residue in the environment and enter into humans living through the food chain, causing negative impact. Here, water-soluble 1.55 nm size nitrogen-doped carbon quantum dots (NCDs) with strong blue fluorescence were synthesized using sodium citrate and adenine. The maximum excitation and emission wavelengths of NCDs were 380 nm and 440 nm, respectively. The above synthesized NCDs were first used for the construction of a fluorescence sensor for glyphosate detection. It was found that Cu2+ could quench the fluorescence of NCDs effectively through the photoinduced electron transfer (PET) process, which was confirmed using fluorescence lifetime measurements. Additionally, the fluorescence was restored with the addition of glyphosate. Hence, a sensitive turn-on fluorescence sensor based on NCDs/Cu2+ for glyphosate analysis was developed. The LODs of glyphosate for water and rice samples were recorded as 0.021 μg/mL and 0.049 μg/mL, respectively. The sensor was applied successfully for ultrasensitive and selective detection of glyphosate in environmental water and rice samples with satisfied recoveries from 82.1% to 113.0% using a simple sample pretreatment technique. The proposed strategy can provide a significant potential for monitoring glyphosate residue in water and agricultural product samples.

In Situ Fluorescence Imaging Reveals Contribution of Cerebral Hydroxyl Radicals in Hyperhomocysteinemia-Induced Alzheimer-like Dementia.

Elevated plasma level of homocysteine, also termed as hyperhomocysteinemia, is acknowledged as a significant and independent risk factor of Alzheimer's disease. However, the mechanistic insight has not been thoroughly elucidated yet. In this work, 3,5-dihydroxybenzyloxy was explored as the unique reaction trigger and integrated into the naphthalimide fluorophore via a carbamate linker to afford a new probe for •OH imaging. •OH treatment induced aromatic hydroxylation and subsequent elimination reaction to release the caged fluorophore, accompanied with a highly specific and sensitive turn-on fluorescence response. Cell imaging results revealed that excess homocysteine triggered overwhelming •OH production, which was mediated by N-methyl-d-aspartate receptor and NADPH oxidase, and the resultant •OH stress further initiated neuronal ferroptosis, also confirmed by western blot analyses. Additionally, hyperhomocysteinemic mouse models were established, and Alzheimer-like dementia of the mice was observed from behavioral tests. Most importantly, with this probe, cerebral •OH fluctuation was in situ visualized in live mice, which positively correlated with the severity of Alzheimer-like dementia induced by hyperhomocysteinemia. These results reveal that cerebral •OH stress may be the critical nexus linking hyperhomocysteinemia and Alzheimer's disease. This work provides a robust fluorescence probe for in situ visualizing the cerebral •OH fluctuations and illuminating critical insights into •OH contributions in brain disorders.

Functionalized Metal-Organic Framework for Selective Fluorometric Detection of Sodium Dodecyl Sulfate and Vitamin B12 Using MOF@Cotton Composites and Lewis Base-Catalyzed Condensation Reaction.

A zirconium(IV)-based metal-organic framework (MOF) fluorophore containing the 2,5-diaminoterephthalic acid (H2BDC-(NH)2) linker was synthesized and characterized. The physicochemically stable, porous (SBET = 504 m2 g-1) MOF (1') exhibited selective and sensitive fluorescence turn-on behavior toward the sodium dodecyl sulfate (SDS) surfactant and turn-off response toward vitamin B12. This is the first ever reported MOF-based dual optical sensor of SDS and vitamin B12. Other competitive analytes did not interfere in the detection of both the analytes. Along with the lowest ever reported limit of detection (LOD) values (LOD for SDS = 108 nM and LOD for vitamin B12 = 45.3 nM), 1' displayed short response time for SDS (50 s) and vitamin B12 (5 s) detection. The MOF was able to detect SDS in various real water samples and vitamin B12 in various bio-fluids (urine and serum) and pH media. A MOF-coated cotton composite was fabricated, which displayed a visible color change under UV light even after treating it with a nanomolar concentration of both the analytes. The sensor displayed excellent reusability up to five cycles of sensing. Various experimental outcomes evidenced that the electrostatic interaction between the -NH2 groups of the linker and the -SO3- group of SDS is the possible reason for the selective SDS sensing. For vitamin B12, the energy transfer from the probe to vitamin B12 resulted in fluorescence quenching. In addition, the catalytic performance of 1' was investigated in the condensation reaction between benzaldehyde derivatives with cyanoacetamide with high yields in ethanol at 70 °C. The solid was used for three cycles with no decrease in its activity and selectivity. PXRD and FESEM analysis before and after the reaction suggested the retention of the crystallinity of 1', thus indicating catalyst stability.

A novel near-infrared fluorescent probe with hemicyanine scaffold for sensitive mitochondrial pH detection and mitophagy study

Mitochondria, as an energy-producing powerhouse in live cells, is considered to be directly linked to cellular health. However, dysfunctional mitochondria and abnormal mitochondria pH would possibly activate mitophagy, cell apoptosis and intercellular acidification process. In this work, we synthesized a novel near infrared fluorescent probe (FNIR-pH) for measurement of mitochondrial pH based on the hemicyanine skeleton as a fluorophore. The FNIR-pH probe functioned as a mitochondrial pH substrate and exhibited quick and sensitive turn-on fluorescence responses to mitochondrial pH in basic solution due to the deprotonation of hydroxy group in the structure. From pH 3.0 to 10.0, the FNIR-pH exhibited almost 100-fold increase in fluorescence intensity at 766 nm wavelength. The FNIR-pH also displayed superior selectivity to various metal ions, excellent photostability, and low cytotoxicity, which facilitated further biological application. Owing to the proper pKa value of 7.2, the FNIR-pH paved the way for real-time monitoring of mitochondria pH changes in live cells and sensitive sensing of mitophagy. Moreover, the FNIR-pH probe was also implemented for fluorescent imaging of tumor-bearing mice to validate its potential application for in vivo imaging of bioanalytes and biomarkers.

Highly sensitive fluorescent turn-on lateral flow strip for chlorothalonil based on an indicator displacement ratiometric fluorescent assay

Nanomaterial based indicator displacement assays (IDA) have demonstrated interesting applications in chemosensing, however, restricted to fluorescence quenching nanomaterials. Fluorescent metal nanocluster (NCs) serving as a receptor in IDA may provide new sensing applications. In this work, we utilized GSH/CD-AuNCs as receptor and kaempferol/Al3+ complex (KAE/Al3+) as indicator to fabricate a new nanomaterial based IDA. Due to inner filter effect (IFE) and photoinduced electron transfer (PET) effect, the fluorescence of GSH/CD-AuNCs was greatly quenched by KAE/Al3+ complex. In the presence of chlorothalonil (CHL), CHL was more likely to enter the cavity than KAE/Al3+ due to its higher affinity with β-CD, thereby restoring the AuNCs fluorescence. Meanwhile, the fluorescence of KAE/Al3+ could be greatly enhanced after β-CD inclusion. Based on the above principle, an indicator displacement ratiometric fluorescent assay (IDRFA) for CHL was successfully constructed. Moreover, we integrated this IDRFA with lateral folw assay (LFA) format to develop a novel fluorescent turn-on IDA-LFA for CHL, which facilitated the naked eye discrimination and sensitive quantitative detection through LFA. Due to the advantages of rapid (completed within 1 min), and highly sensitive (even higher than some immunochromatographic strips), this novel IDA-LFA strip have high potential in rapid screening CHL.

Two Co-Based Metal–Organic Framework Isomers with Similar Metal-Carboxylate Sheets: Turn-On Ratiometric Luminescence Sensing Activities toward Biomarker N-Acetylneuraminic Acid and Discrimination of Ga3+ and In3+

Two supramolecular Co-MOF isomers, namely, {[Co(L)0.5(m-bimb)]·3H2O}n (LCU-115) and {[Co(L)0.5(p-bimb)]·3H2O}n (LCU-116), were synthesized from an amide-containing carboxylic acid N,N″-(3,5-dicarboxylphenyl)benzene-1,4-dicarboxamide (H4L) and two flexible positional isostructural N-containing ligands m-bimb and p-bimb (m-bimb = 1,3-bis((1H-imidazol-1-yl)methyl)benzene; p-bimb = 1,4-bis((1H-imidazol-1-yl)methyl)benzene). The carboxylate ligands connect Co(II) centers to form 2D metal-carboxylate sheets, which are extended further by m-bimb and p-bimb to form a 2D bilayer with parallel stacking (LCU-115) and a 3D framework (LCU-116), respectively. Luminescence measurements indicated that these two complexes exhibited interesting multiresponsive sensing activities toward pH, biomarker N-acetylneuraminic acid, and trivalent cations Ga3+/In3+. They show highly sensitive turn-on fluorescence responses in the acidic range and can also be regarded as on-off-on vapoluminescent sensors to typical acidic and basic gases HCl and Et3N. It is worth noting that these complexes have excellent turn-on ratiometric fluorescence sensing ability for N-acetylneuraminic acid (NANA) with detection limits as low as 7.39 and 8.06 μM, respectively. Furthermore, they were successfully applied for the detection of NANA in simulated urine and serum samples with satisfactory results. For ion detection, LCU-116 could detect both Ga3+ and In3+, while LCU-115 could distinguish Ga3+ from In3+ with the latter showing luminescence quenching. The sensing mechanism was investigated in detail by XRD, UV-vis, EDS, XPS, SEM, and TEM. The results of interday and intraday precision studies gave low RSD values in the range of 1.19-3.53%, ascertaining the reproducibility of these sensors. The recoveries for the sensing analytes in simulated urine/serum or real water are satisfactory from 96.7 to 103.3% (toward NANA) and 96.6 to 115.0% (toward Ga3+ and In3+), indicating that these two complexes also possess acceptable reliability for monitoring in real samples. The results indicated that the supramolecular isomers LCU-115 and LCU-116 are promising material candidates for application in biological and environmental monitoring.

Bilimbi (Averrhoa bilimbi) fruit derived carbon dots for dual sensing of Cu(II) and quinalphos

ABSTRACT Synthesis of highly fluorescent carbon dots from an agro crop through facile one-pot microwave method has been reported. Bilimbi fruit derived carbon dots named as BCDs, exhibited excellent photoluminescent properties and stability. Fluorescence of the system selectively quenched on interaction with Cu(II), due to the complex formation between amine, hydroxyl and carboxyl groups in the surface of the BCDs with Cu(II). The non-fluorescent complex displayed a selective and sensitive turn-on fluorescence behaviour on adding organophosphorus pesticide quinalphos. And hence, the prepared system is used for dual sensing purposes with nanomolar level of detection limits. The limit of detection of Cu(II) and quinalphos was estimated as 115 nM and 510 nM, respectively. The entire detection process was visible under UV light of 365 nm. Moreover, the BCDs@Cu(II) nanoprobe was effectively applied as fluorescence sensor of quinalphos in real samples of rice and tea where its presence is frequently reported, with good level of recovery percentages.

An AIE luminogen self-assembled nanoprobe for efficient monitoring of the concentration and structural transition of human serum albumin

Monitoring the content and structural transition of human serum albumin (HSA) is valuable for diagnosing hypoalbuminemia and albuminuria and elucidating its transport function, respectively. Herein, we developed a multifunctional and efficient nanoprobe based on the self-assembly of an amphiphilic aggregation-induced emission luminogen, TPNN, for HSA detection and structure monitoring. TPNN molecules formed loose self-assembly in aqueous media and emitted faint fluorescence due to vigorous intramolecular motion. In the presence of HSA, a remarkable fluorescence enhancement accompanied by dissociation of TPNN assembly was observed due to the site-specific binding of TPNN to the middle long-narrow pocket of HSA. This binding blocked the intramolecular motion while producing a sensitive, selective, fast and stable fluorescence turn-on response to HSA, making TPNN assembly suitable for the HSA assay. TPNN assemblies also showed superior selectivity to HSA than bovine serum albumin (BSA) due to the distinct binding modes, and enabled us to distinguish the two homologous proteins. Furthermore, the generated TPNN-HSA complex underwent stepwise fluorescence quenching in the presence of protein denaturants and proteases, which revealed the process and kinetics of HSA unfolding and cleavage. TPNN assembly provides a powerful tool for accurate quantification of HSA in serum and urine and real-time monitoring of HSA structural transition.

Photophysical characterization of coumarin and rhodanine derivatives as viscosity sensitive fluorescence turn-on probes

In this study, coumarin and benzylidene rhodanine derivatives, Cou-A and Rho-A, were synthesized and characterized as viscosity-sensitive molecular platforms. Cou-A and Rho-A showed quenched fluorescence behaviour in low viscosity polar solvents. However, when the solvent viscosity was increased from 2.5 cP to 1150 cP using water-glycerol mixtures, both probes displayed fluorescence turn-on responses. Cou-A showed a 27-fold increase in fluorescence at 476 nm, while Rho-A showed a 508-fold increase in fluorescence at 575 nm. Both Cou-A and Rho-A showed a linear relationship between fluorescence intensity (log I) and solvent viscosity (log η), with viscosity sensitivity values of 0.554 and 0.996, respectively. The reported probes' fluorescence profiles were unaffected by the presence of metal ions or the pH of the solvent medium. Further studies suggest that the fluorescence quantum yield of these probes is dependent on both viscosity and temperature.

A Nile red-based near-infrared fluorescent probe for the detection of superoxide radical anion in living cells

Reactive oxygen species (ROS) are important products of aerobic metabolism and play important roles in many physiological and pathological processes. Among the ROS, superoxide radical anion (O 2 •‒ ) is a primary ROS and precursor of a few other kinds of ROS. O 2 •‒ is widely involved in regulating cell functions and is a critical biomarker for monitoring cellular stress responses. Thus, a real-time quantitative detection of O 2 •‒ is of great significance in ascertaining the pathological roles of O 2 •‒ in related diseases. Herein we report a near-infrared (NIR) fluorescent probe NR1 for the sensitive detection and imaging of O 2 •‒ , which is rationally designed and synthesized through an esterification reaction of a Nile red dye and the diphenyl phosphinyl group. The NR1 probe exploits the strong nucleophilic mechanism of O 2 •‒ as a novel detection strategy, which leads to the cleavage of NR1 to yield Nile red as the fluorescence reporter and diphenyl phosphinyl functional group as the responsive unit. The developed NR1 probe exhibits highly sensitive, selective and rapid fluorescence turn-on response on addition of O 2 •‒ in the presence of potential ROS interfering species. Furthermore, the probe NR1 is successfully applied in imaging of various cancer cell lines (4T1 and Hela) and excellent imaging ability of O 2 •‒ in vitro is achieved. Biocompability of NR1 is also evaluated through MTS assay. The present study provides a novel strategy for molecular design of NIR fluorescent probe and the resulting probe shows great potential for practical application in complex bio-systems to study the development and progression of related diseases.

Fluorescence turn-on Cu2-xSe@HA-rhodamine 6G FRET nanoprobe for hyaluronidase detection and imaging.

The application of nanostructures to design fluorescence resonance energy transfer (FRET) based sensing platforms has been greatly concerned with the demand for sensitive and selective detection of biomolecules. Here, a novel sensitive turn-on fluorescence strategy based on the FRET mechanism has been designed for hyaluronidase (HAase) detection through the modulation of Cu2-xSe@HA-Rh6G nanoprobe fabricated by self-assembly of rhodamine 6G (Rh6G) together with Cu2-xSe@HA nanoparticles through electrostatic adsorption. The Cu2-xSe@HA had extensive localized surface plasma resonance (LSPR) absorption in the wide range of ultraviolet (UV) to near-infrared (NIR) wavelengths and showed good light capture characteristics, which can be acted as good acceptors in the FRET interactions with Rh6G, inducing its efficient fluorescence quenching. In the presence of HAase, the FRET process was disrupted and the fluorescence signal was recovered. In the range of 0.1-10.0U/mL, the fluorescence recovery of Rh6G showed a good linear relationship with the concentration of HAase, and the detection limit was 0.06U/mL. The sensing platform has been used for HAase detection in real urine samples and cancer cells imaging.

HClO-Activated Near-Infrared Fluorogenic Aza-BODIPY-Bisferrocene Triad with High Turn-on Ratio for In Vivo Biosensing.

Optically monitoring hypochlorous acid (HClO) in living body favors diagnosis and study of inflammatory diseases. However, this has been hampered by limited strategies to develop highly fluorogenic tools in the deep-penetration near-infrared spectrum. Herein, a near-infrared aza-BODIPY-bisferrocene triad Fc2 -CBDP that unexpectedly achieves an exceptionally sensitive and selective fluorescence turn-on (>220-fold) response toward HClO through single-ferrocene oxidation and boron-alkynyl hydrolysis cascade is reported. Mechanism insight shows that Fc2 -CBDP features "enhanced charge transfer"-caused quenching due to intramolecular bisferrocene electronic coupling, which is decoupled in the reaction with HClO. The utility of Fc2 -CBDP for intracellular HClO imaging is evaluated and, more importantly, in vivo high-contrast deep-tissue imaging of lymphatic inflammation and colitis is realized. This work provides new insights into both HClO and ferrocene chemistry, and extends the reach of fluorogenic strategies in the near-infrared biosensing.

Selective and sensitive fluorescence turn-on detection of bilirubin using resorcinol-sucrose derived carbon dot

Carbon dots have been prepared from resorcinol and sucrose (rsCDs) hydrothermally, which show green emission at 525 nm with a fluorescence quantum yield (PLQY) of 17.2%. The intense emission of rsCDs is quenched upon the addition of Cu2+. In the presence of bilirubin (BR), the emission intensity is enhanced due to the competitive binding of Cu2+ with bilirubin and hence releasing rsCDs to the sensing medium. It is the first time report on turn-on fluorescence sensing towards BR with a detection limit of 85 nM. Even in the presence of other comparable biomolecules, the sensor is selective and ultrasensitive to bilirubin. A cellulose paper-based sensor strip has also been designed for the naked-eye detection of BR in blood serum. Due to the specific recognition of this rsCDs towards BR, it can be applied to detect BR in practical human serum samples.

Selective and sensitive investigation of aluminium contamination from cookware based on novel water-soluble fluorescence turn-on chemosensor

The ubiquitous use of aluminium cookware raises serious concerns about the introduction of aluminium by leaching upon prolonged cooking, especially in acidic foods. To mimic a consumer-relevant exposure scenario, an experimental model design was conceptualized for the estimation of leaching from aluminium sheets in acidic additives under variable cooking conditions. A novel water-soluble receptor (Z)-2-((2-hydroxy-3-methoxybenzylidene)amino)-2-(hydroxymethyl)propane-1,3-diol (VT) was synthesized, which gave a selective and sensitive fluorescence turn-on response towards aluminium ions. The 1:1 binding stoichiometry between VT and Al3+ has been confirmed by Job's plot, which is further supported by 1H NMR, 27Al NMR, HRMS, and DFT studies. Test strips were fabricated for quick semi-quantitative estimation of aluminium ions under UV irradiation. Leaching studies show that on increasing the acidity of solution of acidic additives results in an increased aluminium leaching ranging from 4.95 to 11.43 mg/h. Surface morphology by SEM and AFM shows the pitting corrosion of Al sheets.