In this paper, an extraction method for measuring impact ionization-induced hole current in gallium nitride (GaN) metal–insulator–semiconductor high electron mobility transistors (MIS-HEMTs) is proposed. The results show that the non-monotonic impact ionization current characteristic can be easily acquired by the extraction method. Further, different hot-carrier stress (HCS) conditions can be obtained based on the I G–V G curve, and the reliability tests can act as verification of the impact-ionization curve. In addition, electrical reliability tests indicate that the threshold voltage (V TH) shift and on-state current (I on) degradation in the MIS-HEMTs have a positive correlation to impact ionization-generated hole current. During HCS operation, the V TH will shift positively and I on decreases due to hot electrons trapping into the GaN layer. This model is validated by TCAD simulation.