Tissue Selenium Research Articles

Evaluation of selenium source on nursery pig growth performance, serum and tissue selenium concentrations, and serum antioxidant status.

A total of 3,888 pigs (337 × 1050, PIC, Hendersonville, TN; initially 6.0 ± 0.23 kg) were used in a 35-d study. At the time of placement, pens of pigs were weighed and allotted to one of three dietary treatments in a randomized complete block design with a blocking structure including sow farm origin, date of entry into the facility, and average pen body weight. A total of 144 pens were used with 72 double-sided 5-hole stainless steel fence line feeders, with one feeder serving as the experimental unit. For each feeder, 1 pen contained 27 gilts, and 1 pen contained 27 barrows. There were 24 replicates per dietary treatment. Diets were fed in three phases, and all contained 0.3 mg/kg added Se. A common phase 1 diet contained added Se from sodium selenite and was fed in pelleted form to all pigs from day 7 to approximately day 0. Three Se sources sodium selenite, Se yeast, and hydroxy-selenomethionine (OH-SeMet) were used to formulate three experimental diets in meal form for phase 2 (days 0 to 14) and phase 3 (days 14 to 35). During the pre-treatment period (days 7 to 0), there was a tendency (P = 0.097) of a difference in average daily feed intake between treatments, although no significant pairwise differences were observed (P > 0.05). There were no other differences in growth performance between treatments from days 7 to 0. Clinical disease attributed to Streptococcus suis was observed within the trial between days 0 and 14, and water-soluble antimicrobial therapy was administered to all treatment groups for 7 d. From days 0 to 35, pigs fed OH-SeMet tended to have decreased average daily gain (P < 0.10) and increased (P < 0.05) serum and tissue selenium concentration compared to other treatments. There was marginally significant evidence of a source × day interaction (P = 0.027) for total antioxidant capacity where the numerical increase over time was less for the OH-SeMet than sodium selenite or selenium yeast treatments. There was no difference (P > 0.05) in antioxidant status as measured by serum glutathione peroxidase or thiobarbituric acid reactive substances assay between treatments. In summary, compared to sodium selenite and selenium yeast, OH-SeMet may have a greater bioavailability as indicated by increased serum and tissue selenium concentration; however, antioxidant status was similar between treatments and OH-SeMet tended to reduce growth performance compared with pigs fed sodium selenite.

Effect of dietary oregano essential oil on growth, meat quality, selenium concentration and biochemical traits serum of pigs

Natural substances of plant origin play an important role in the protection of animal health and the improvement of the quality and health safety of food products of animal origin. The research was carried out to determine the effect oregano essential oil, as a feed additive on the production effects, quality and chemical composition of meat, as well as on the level of selenium in pig tissues and selected serum biochemical indicators. The study did not show a significant effect of the preparation containing oregano oil used in the feeding of fattening pigs on the body weight of fattening pigs, their growth rate, as well as feed intake and utilization. Chemical analysis showed that pigs receiving the addition of oregano oil achieved significantly higher (p0.05) content of dry matter and protein in the Longissimus dorsi (LD) muscle. The LD muscle colour assessment showed that supplementation of the pig diet during the fattening period with oregano oil had a significant effect on the yellow colour component of meat (higher b* values; p0.05). Supplementation with oregano oil contributed to a significant reduction in serum triglyceride concentration (p0.05). The results of this study suggest that supplementation of the pigs' diet with oregano oil contributes to the increase in the yellowness of meat and the content of dry matter and protein as well as to the decrease in the concentration of triglycerides in the serum.

Study of the effect of a seven -containing drug on the concentration of selenium in the organs and tissues of the lambs of the Romanov breed in the conditions of industrial complexes North-Western region

Purpose: to study the effects of a selenic -containing drug on the concentration of selenium in blood serum, organs and tissues of lambs of the Romanov breed.Materials and methods. In the course of the study, 120 novels of the Romanov breed and 80 lambs obtained from the data of disemeters were used. The formation of control and experimental groups was carried out taking into account the principle of pair analysis. In experiments on assessing the effect of the drug, clinically healthy animals were used on the body. During the study, four groups of lambs were formed at the age of 30 days. The mothers of the first group during the period of suyagnity received the drug “e-sel” at the rate of 1 ml per 50 kg of live weight; The lambs of the first experimental group introduced the drug “E-sel” at the rate of 0.2 ml per 10 kg of live weight according to the instructions. The second group was a control - a physiological solution was administered in the same quantity as the drug with experimental animals; Feeding was carried out by the main diet. The studies were carried out in the period before the chopping (4 months of age) was carried out. Blood fence was carried out from the jugular vein. The concentration of selenium in blood serum lambs was determined by fluorimetrically with 2.3-diaminonaftalin. After the chopping, the lambs were slaughtered. The concentration of selenium in the organs and tissues of the lamb was examined by atomic-adsorption spectrometry.Results. It was established that the concentration of selenium in the lambs of the first experimental group increased, starting from the 60s from the moment of birth. At the same time, in the lambs of the first experimental group, the concentration increased 2.84 times by the 60th knocks compared to control, 4.08 times compared to control by the 90s from the moment of birth and 8.5 times by 120 -It day from the moment of birth. The concentration of selenium in the kidneys, liver and muscles (the broadest back muscle) of the first experimental group significantly exceeded the values of control. The maximum concentration was noted in the kidneys. No reliable changes were noted between the 1st and 2nd experimental groups in the concentrations of selenium in the kidneys and liver. The concentration of selenium in the liver of the experimental group was 2.94 times higher in comparison with control. In the kidneys, the concentration of selenium was 2.26 times higher for the experimental group in comparison with control. It is noted that an increase in the concentration of selenium in the muscles exceeded control indicators of 4.59, respectively.

The Contents of Some Trace Elements (As, Br, Cu, Hg, Se, and Zn) in Mytilus galloprovincialis Mussels From Agigea Port, Romania

This study aimed at assessing the accumulation of some trace elements (arsenic, bromine, copper, mercury, selenium, and zinc) in the soft tissue of Mediterranean mussels (Mytilus galloprovincialis) from the Black Sea, near Agigea Port. The mussel samples were collected on a monthly basis between February and June 2019 from two stations located in front of the Danube–Black Sea Canal lock, along the two artificial dikes. The mussels (260 samples) were divided into three classes according to their length: A (3−5.9 cm), B (6−8.9 cm), and C (9−12 cm). The concentrations (dry basis) of As (12.5 ± 3.0 mg/kg), Br (173.6 ± 53.9 mg/kg), Cu (11.2 ± 2.5 mg/kg), Hg (0.02 ± 0.02 mg/kg), Se (2.9 ± 1.1 mg/kg), and Zn (179.5 ± 2.5 mg/kg) were determined using spectrometric techniques. The mean daily values of the physicochemical parameters of seawater (temperature, salinity, pH, chlorophyll a content, and dissolved oxygen content) within the study area were reported. Higher element concentrations were generally found for mussels from size classes A and B compared with those of the larger and older mussels from class C. Principal component analysis (PCA) suggested that the phytoplankton represented an important source of As and Br for the smaller mussels collected in February (when the levels of seawater temperature were lower and those of pH and salinity higher) compared to the larger mussels collected in June. Moreover, the multivariate analysis suggested that Cu, Se, Zn, and Br originated from similar anthropogenic sources and their higher concentrations reported in February and June were obtained at lower levels of seawater salinity. The contents of Cu, Zn, Se, As, and Hg in the mussel soft tissue were lower than the maximum permissible levels.

Molecular Fates of Organometallic Mercury in Human Brain.

Mercury is ubiquitous in the environment, with rising levels due to pollution and climate change being a current global concern. Many mercury compounds are notorious for their toxicity, with the potential of organometallic mercury compounds for devastating effects on the structures and functions of the central nervous system being of particular concern. Chronic exposure of human populations to low levels of methylmercury compounds occurs through consumption of fish and other seafood, although the health consequences, if any, from this exposure remain controversial. We have used high energy resolution fluorescence detected X-ray absorption spectroscopy to determine the speciation of mercury and selenium in human brain tissue. We show that the molecular fate of mercury differs dramatically between individuals who suffered acute organometallic mercury exposure (poisoning) and individuals with chronic low-level exposure from a diet rich in marine fish. For long-term low-level methylmercury exposure from fish consumption, mercury speciation in brain tissue shows methylmercury coordinated to an aliphatic thiolate, resembling the coordination environment observed in marine fish. In marked contrast, for short-term high-level exposure, we observe the presence of biologically less available mercuric selenide deposits, confirmed by X-ray fluorescence imaging, as well as mercury(II)-bis-thiolate complexes, which may be signatures of severe poisoning in humans. These differences between low-level and high-level exposures challenge the relevance of studies involving acute exposure as a proxy for low-level chronic exposure.

Total Mercury, Total Selenium, and Monomethylmercury Relationships in Multiple Age Cohorts and Tissues of Steller Sea Lions (Eumetopias jubatus).

Steller sea lion (Eumetopias jubatus) tissue mercury concentrations increasingly above thresholds of concern occur in regions of Alaska where lack of population recovery is noted. Selenium-monomethylmercury interactions may mitigate toxicosis but may also result in functional selenium deficiency, impacting essential selenium-dependent processes. Physiologically driven differences in tissue concentrations (organotropism) of total mercury ([THg]), total selenium ([TSe]), and monomethylmercury ([MeHg+ ]) confound interpretation for various age cohorts. Archived tissues from Alaska Steller sea lions (2002-2016) were used to compare [THg], [MeHg+ ], and [TSe] across age cohorts and tissue types. Liver [THg] ranged from 0.05 to 63.7 µg/g. Fetal and pup livers had significantly lower [THg] and [TSe], higher percentage MeHg+ , and greater range of molar TSe:THg than subadult and adult livers. Molar Se:MeHg+ ratios, including Se in excess of nonmethylmercury, were dependent on [MeHg+ ] in fetuses and pups. While [THg] varied significantly by muscle type (heart vs. skeletal) and anatomical location, concentrations were strongly correlated. Biomagnification and/or bioaccumulation of THg in liver of older animals confounded comparison with other tissues; however, in fetal and pup liver [THg] correlated with other tissues. In contrast, liver [MeHg+ ] correlated with other tissues across all age classes. Fetal and pup tissues, which reflect in utero exposure and are subject to limited bioaccumulation, are ideal for assessing mercury exposure related to dam diet, including intertissue comparison, and represent key cohorts of concern. Evaluating [MeHg+ ] and [TSe] in tissues from multiple age cohorts allows better intertissue comparison, providing insight into time courses, routes of exposure, and potential for adverse effects. Environ Toxicol Chem 2022;41:1477-1489. © 2022 SETAC.

Productive Performance, Serum Antioxidant Status, Tissue Selenium Deposition, and Gut Health Analysis of Broiler Chickens Supplemented with Selenium and Probiotics-A Pilot Study.

Simple SummaryFor several years, the benefits of dietary selenium or probiotics on growth and development of broilers have been reported. However, the effect of a combination of different forms of selenium and probiotics on broilers remains to be seen. Thus, the hypothesis of this study was that supplementing selenium and probiotics have positive synergistic effects and interactions on growth performance, antioxidant capacity, tissue selenium deposition, and intestinal health of broilers. The results of this study demonstrate that selenium and probiotics have positive interactions on the tissue selenium content, duodenum, and jejunum development, as well as the composition of cecum microbial, and there were no significant interactions on the growth performance, and antioxidant capacity. Overall, our findings suggest that the combination of organic selenium and probiotics is superior to inorganic or organic selenium alone in poultry production.The effect and interaction of dietary selenium (Se) and probiotics on three yellow chicken growth performance, tissue Se content, antioxidant capacity, and gut health were studied from 0 to 70 days of age. A total of 400 one-day-old broilers were distributed into four groups (I-Se, O-Se, I-Se + pros, and O-Se + pros groups) consisting of a 2 × 2 factorial design. The main factors were the source of Se (I-Se = inorganic Se: 0.2 mg/kg sodium selenite; O-Se = organic Se: 0.2 mg/kg Selenium yeast) and the level of probiotics (0.5% EM or 0% EM, the component of EM mainly includes Lactobacillus and Yeast at the dose of 2 × 108 cfu/kg and 3 × 107 cfu/kg, respectively). Each treatment had 5 duplicates consisting of 20 broilers. The results showed that the I-Se group had a greater (p < 0.05) ratio of feed: weight gain (F/G) of broilers at Starter (0–35 d) than the other treatments. Compared to the I-Se group, the O-Se group increased (p < 0.05) Se concentrations in the liver, pancreas, breast muscles, thigh muscle, and the activity of total antioxidative capacity (T-AOC) in serum, as well as the relative abundance of Barnesiella and Lactobacillus in cecum. Meanwhile, probiotics enhanced (p < 0.05) Se concentrations in the pancreas, thigh muscle, serum, and the activity of T-AOC and glutathione peroxidase (GSH-Px), the duodenum’s ratio of villi height to crypt depth (V/C), the jejunum villus height and V/C, and the ileum’s villus height. Furthermore, the significant interactions (p < 0.05) between Se sources and the level of probiotics were observed in Se concentrations in the pancreas, thigh muscle, serum, crypt depth of duodenum, and villus height of jejunum of birds, and Barnesiella abundance in the cecal. In conclusion, our results demonstrate that the combination of O-Se + pros can improve broiler early growth performance, tissue Se content in the pancreas, thigh muscle, and serum, promote intestinal development, and regulate the composition of intestinal flora, suggesting a better combination. These findings provide an effective method of nutrient combination addition to improving the performance of three yellow chickens.

114 Evaluation of Selenium Source on Nursery Pig Growth Performance, Serum and Tissue Selenium Concentrations, and Serum Antioxidant Status

Abstract A total of 3,888 pigs (337×1050, PIC, Hendersonville, TN; 6.0 kg at weaning) were used in a 42-d study to determine the influence of Se source on nursery pig selenium status and growth performance. At placement, pens of pigs were weighed and allotted to 1 of 3 dietary treatments in a randomized complete block design with blocking structure including sow farm origin, date of entry, and average pen BW. A total of 144 pens were used with 72 double-sided 5-hole stainless steel, fence-line feeders, with feeder serving as the experimental unit. For each feeder, 1 pen contained 27 gilts and the other 27 barrows. There were 24 replicates per treatment. Diets were fed in 3 phases, and all contained 0.3 ppm added Se. A common phase 1 diet contained added Se from sodium selenite and was fed in pelleted form to all pigs for 7 d prior to initiation of treatment diets. Three Se sources [sodium selenite; Se yeast; and hydroxy-selenomethionine (OH-SeMet)] were used to formulate 3 experimental treatments in meal form for phase 2 (d 7 to 21) and phase 3 (d 21 to 42). During the common phase 1 period, ADFI tended (P &amp;lt; 0.10) to be different (107, 103, and 102 g/d) for sodium selenite, Se yeast, and OH-SeMet, respectively. From d 7 to 42 (treatment period), pigs fed OH-SeMet tended to have decreased ADG (P &amp;lt; 0.10) and had increased (P &amp;lt; 0.05) serum, liver, and muscle selenium concentration compared with other treatments. There was no difference (P &amp;gt; 0.05) in antioxidant status as measured by serum GSH-Px or TBARS. In summary, compared with sodium selenite and selenium yeast, OH-SeMet had greater bioavailability as indicated by increased serum and tissue selenium concentration; however, antioxidant status was similar between treatments and OH-SeMet tended to reduce growth performance compared with pigs fed sodium selenite.

Growth and shelf life of basil in response to selenium fertilization

Selenium is essential for human metabolism and its enrichment in vegetables plays an effective role in providing the body with selenium requirements. This factorial experiment was conducted in a greenhouse at Shahrekord University in 2019 to study the effects of selenium on basil growth and shelf life in the conditions of feeding with poultry manure (PM) and chemical fertilizers (CF). Experimental factors included two types of soil amended for plant nutrition (PM and CF) and five selenium concentrations (0, 1.5, 3, 4.5, and 6 mg L − 1) applied as foliar sprays. The results showed that shoot fresh weight in plants receiving PM was 16.6% more than CF fertilizers and foliar application of selenium had no effect on fresh weight. In the PM applied conditions, with the increase of selenium, the chlorophyll b content increased and then decreased. Increasing selenium improved its accumulation in the basil tissue, and the amount of selenium accumulated with poultry manure was more than that accumulated with chemical fertilizer. Whereas, the greatest weight loss in storage was observed in high selenium concentration. In plants receiving PM, increasing concentration of selenium sprays improved the shelf life, then decreased. However, in the amended soil with CF, the shelf life did not change by selenium, except in 1.5 mg L − 1 concentration. It is concluded that the foliar application of moderate amounts of selenium in poultry manure conditions helps to produce more basil, relatively good enrichment of selenium in plant tissue and maintain its optimal quality during the storage.

Direct solid sampling of biological species for the rapid determination of selenium by high-resolution continuum source graphite furnace atomic absorption spectrometry

A direct solid sampling method based on high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS) for the determination of selenium (Se) in biological tissues was optimized. The main analytical line of Se at 196.0267 nm was used to carry out all HR-CS AAS measurements. Different chemical modifiers were evaluated to prevent the loss of Se during the application of the GFAAS temperature program and avoid the interferences due to the presence of phosphorus compounds in sample matrix. The best results were achieved using ruthenium coated platforms and a palladium nanoparticle suspension (Pd NP) as co-injected modifier. Calibration was performed using aqueous standard solutions of Se(IV). For solid sampling, the optimal range of sample mass was between 0.2 and 0.8 mg. The limit of detection (LOD) was 0.06 ng (0.075 μg g−1 using a sample mass of 0.8 mg). The developed solid sampling HR-CS GFAAS method was used for Se determination in two certified reference materials of dogfish tissues and lyophilized and powdered real samples of fish tissues and chicken liver. The precision obtained in these analyses, expressed as relative standard deviation (RSD), was between 5.5 and 8.6% (n = 6). For validation purposes, the results obtained by the solid sampling HR-CS GFAAS method were compared with those found performing the analysis by HR-CS hydride generation AAS (HR-CS HGAAS) after microwave acid digestion of the samples. The Se concentrations obtained for both methods agreed at a 95% confidence level (Student's t-test), indicating the suitability of the proposed solid sampling HR-CS GFAAS method to determine Se in biological samples.

СЕЛЕН В НАНОФОРМЕ: ТОКСИЧНОСТЬ И БЕЗОПАСНОСТЬ

The review is devoted to the compilation of data on the toxicity and safety of using nanosized forms of selenium. It is shown that in nature selenium exists mainly in the form of selenate (Se6+), selenite (Se4+), selenide (Se2–) and elemen-tal selenium (Se0), while the latter is insoluble in aqueous media, less toxic and biologically inert. Elemental selenium in the form of nanosized particles is not only biocompatible, but also has antitumor and antimicrobial activity. It was shown that elemental selenium in the form of nanoparticles can modulate the activity of the antioxidant and detoxification systems. A data on the dose-dependent effect of selenium in nanosized form was presented. It was demonstrated that selenium nanoparticles in high concentrations (above 2 mg of Se per 1 kg of animal weight) can cause the development of selenium-induced toxicity in mammals. It was shown that elemental selenium in the form of nanoparticles can affect immunoregulation, reproductive func-tion, kidney and liver function, can modulate the activity of the antioxidant and detoxification systems, and in high concentrations (above 2 mg of Se per 1 kg of animal weight) it can cause the development of selenium-induced toxicity both in mammals and fish. At the same time, it was shown that for fish selenium nanoparticles are more toxic than inorganic selenium and cause a more acute reaction of the body to exposure to even low concentrations, which is possibly associated with hyperaccumulation of selenium in tissues. This fact once again reminds us of the need to take into account the problems of ecotoxicity of selenium nanocomposites.

Kidney tissue selenium levels of Toxocara canis infected mice given Nigella sativa

Nigella sativa (NS) has a protective effect on cellular damage caused by oxidative stress. Selenium has an antioxidative effect. Toxocara canis is one of the nematodes causing visceral larva migrans. Men infected with this parasite ingesting an embryonic egg. It is more common in children between 1-4 years than adults. From the ingested embryonic egg, the larvae released in the small intestine and they migrate to so many organs such as liver, spleen, kidney, lung, brain, retina of the eye, pancreas and causing lesions. In particular, it is known to cause intense damage to kidney tissue. In this study mice with Toxocara canis infection were administered Nigella sativa in prophylactic and treatment doses (100 and 200 mg/kg body weight) and selenium levels were determined in their kidney tissues. In the healthy control group, kidney selenium levels were 980,46±236,68 ng/g and in mice infected with Toxocara canis 1240,15±315,77 ng/g. Kidney tissue Se levels of mice given NS in two different doses for treatment (Treated N100, Treated N200) and prophylaxis (Prophylactic N100 and ProphylacticN200) respectively are 1297,95±354,37; 1361,29±410,46 ng/g; 1148,55±240,28 ng/g and 1465,81±450,25 ng/g. Kidney tissue selenium levels were high in both treatment and prophylaxis dose NS given mice. In conclusion, Nigella sativa can cause increases in kidney tissue selenium levels depends on given doses.

Effects of bacterial organic selenium, selenium yeast and sodium selenite on antioxidant enzymes activity, serum biochemical parameters, and selenium concentration in Lohman brown-classic hens

This study compares the effects of sodium selenite, selenium yeast, and enriched bacterial organic selenium protein on antioxidant enzyme activity, serum biochemical profiles, and egg yolk, serum, and tissue selenium concentration in laying hens. In a 112-d experiment, 144 Lohman Brown Classic hens, 23-wks old were divided into four equal groups, each has six replicates. They were assigned to 4 treatments: 1) a basal diet (Con), 2) Con plus 0.3mg/kg feed sodium selenite (SS); 3) Con plus 0.3mg/kg feed Se-yeast (SY): 4) Con plus 0.3mg/kg feed bacterial enriched organic Se protein (ADS18) from Stenotrophomonas maltophilia bacteria. On d 116, hens were euthanized (slaughtered) to obtain blood (serum), liver organ, and breast tissue to measure antioxidant enzyme activity, biochemical profiles, and selenium concentration. The results show that antioxidant enzyme activity of hens was increased when fed bacterial organic Se (ADS18), resulting in a significant (P < 0.05) increase in serum GSH-Px, SOD, and CAT activity compared to other treatment groups. However, ADS18 and SY supplementation increase (P < 0.05) hepatic TAC, GSH-Px, and CAT activity, unlike the SS and Con group. Similarly, dietary Se treatment reduced total cholesterol and serum triglycerides concentrations significantly (P < 0.05) compared to the Con group. At 16 and 18weeks, selenium concentration in hen egg yolks supplemented with dietary Se was higher (P < 0.05) than in Con, with similar patterns in breast tissue and serum. Supplementation with bacterial organic Se (ADS18) improved antioxidant enzyme activity, decreased total serum cholesterol and serum lipids, and increased Se deposition in egg yolk, tissue, and serum. Hence, organic Se may be considered a viable source of Se in laying hens.

Comparison of selenomethionine and hydroxyselenomethionine on tissue selenium retention, and antioxidative capacity of giant grouper, Epinephelus lanceolatus , fed diet with soybean meal

The study aims to evaluate the effects of two organic selenium (Se) sources, selenomethionine (SeMet) and hydroxyselenomethionine (OH-SeMet), on tissue Se retention, and antioxidative capacity of giant grouper, Epinephelus lanceolatus, fed diets with soybean meal (SBM). The control diet containing 32.92% SBM (replaced 40% fish meal protein) was supplemented with 0.3, 0.6 and 1.0 mg Se/kg of SeMet and OH-SeMet. A reference diet with fish meal (FM) as main protein source was also included for comparison. The eight experimental diets were each fed to triplicate groups of grouper juveniles (initial weight: 25.99 ± 0.04 g) in a recirculation system with seawater for 8 weeks. The Se concentrations in whole body, muscle and liver were dependent on dietary Se concentrations, but not on its supplemental forms. Higher hepatic glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), but lower malondialdehyde (MDA) content, were observed in fish fed the reference diet than in fish fed the control diet. The results indicated that dietary FM protein replaced by SBM at 40% depressed growth performance, Se retention and antioxidative capacity of giant grouper. Both Se sources supplementation enhanced Se retention and antioxidative capacity, but not growth performance of the fish.

Association between trace elements in cancerous and non-cancerous tissues with the risk of breast cancers in western Iran.

This study aimed to assess the tissue content of essential and toxic metals including lead (Pb), cadmium (Cd), arsenic (As), silver (Ag), aluminum (Al), chromium (Cr), copper (Cu), iron (Fe), selenium (Se), and zinc (Zn) in the breast cancerous tissues compared to the non-cancerous tissue. The biopsy specimens of 63 breast cancers along with 63 adjacent healthy tissues in Kurdistan Province, Iran, were collected from 2019 to 2020 and assayed using ICP-MS (Agilent 7900). The results of the Mann-Whitney test illustrated that the concentration of Pb, Cd, As, Cr, Cu, and Se were significantly elevated in cancerous tissue (p < 0.05), while Zn was the only trace element with higher levels in healthy subjects (p < 0.05). Moreover, weak to moderate correlations between elements were observed in the cancerous group including Al-Cr (r=0.60), As-Cu (r=0.52), and Cu-Se (r=0.56). In contrast, no correlation over 0.50 was found between trace elements in the non-cancerous group. Raw risk differences (RDs) accounted for a significant effect for Pb, Cd, As, Ag, Cr, Se, and Zn on the development of breast cancer. In conclusion, elevated levels of As, Cd, Cu, Pb, and Se may contribute to enhancing the risk of breast cancer.

Effects of Selenium Auricularia cornea Culture Supplementation on Growth Performance, Antioxidant Status, Tissue Selenium Concentration and Meat Quality in Growing-Finishing Pigs.

Simple SummarySelenium Auricularia cornea culture (SAC) is a dried product via full fermentation, containing organic-Se, Auricularia cornea (AC) mycelium, and various metabolites of AC. The objective of this study was to evaluate whether SAC could effectively improve the health, growth, meat quality, and oxidative stability of meat in growing-finishing pigs. Currently, dietary SAC supplementation positively impacts growth performance and oxidative stability of fresh meat.Selenium Auricularia cornea culture (SAC) is a new source of organic selenium. Two experiments were conducted to determine the available energy of SAC fed to pigs and to evaluate the effects of dietary SAC supplementation on growth performance, serum biochemical profiles, fecal short chain fatty acids (SCFA), meat quality, tissue selenium concentration, and oxidative stability of fresh meat in growing-finishing pigs. In Experiment (Exp.) 1, 12 barrows with average body weight (BW) of 42.40 ± 5.30 kg were randomly allotted to two groups and fed the basal diet and SAC-supplemented diet, individually. In Exp. 2, 96 growing-finishing pigs (BW: 91.96 ± 7.55 kg) were grouped into four dietary treatments; each treatment contained six replicates with four pigs per replicate. The four treatments fed a control diet and three experimental diets supplemented with 0.6%, 1.2%, and 2.4% SAC, respectively. The trial lasted for 45 days. The results revealed that digestible energy (DE) of SAC was 11.21 MJ/kg. The average daily gain (ADG) was improved in pigs fed 1.2% and 2.4% SAC during day 24 to 45 and the overall period. Dietary 1.2% and 2.4% SAC supplementation had a lower F/G (p < 0.05) than the control diet during different stages. Dietary SAC supplementation increased fecal butyrate contents (p < 0.05), and pigs fed 1.2% and 2.4% SAC diets had a higher MCT1 mRNA expression (p = 0.04) in the colon. Pigs fed 2.4% SAC had higher GSH-Px contents (p < 0.05) in serum, liver, and longissimus dorsi muscle (LDM) than those in the control group. The 2.4% SAC-supplemented group revealed a higher Se content (p < 0.05) in LDM and a lower MDA concentration (p < 0.05) in fresh meat during the simulated retail display on day six. In conclusion, this study suggested that SAC was more effective in improving growth, enhancing the antioxidant status, depositing Se in muscle, and increasing meat oxidative stability of pigs.

Organic Selenium Increased Gilts Antioxidant Capacity, Immune Function, and Changed Intestinal Microbiota.

Selenium is an indispensable essential micronutrient for humans and animals, and it can affect biological functions by combining into selenoproteins. The purpose of this study was to investigate the effects of 2-hydroxy-4-methylselenobutanoic acid (HMSeBA) on the antioxidant performance, immune function, and intestinal microbiota composition of gilts. From weaning to the 19th day after the second estrus, 36 gilts (Duroc × Landrace × Yorkshire) were assigned to three treatments: control group, sodium selenite group (0.3 mg Se/kg Na2SeO3), and HMSeBA group (0.3 mg Se/kg HMSeBA). Dietary supplementation with HMSeBA improved the gilts tissue selenium content (except in the thymus) and selenoprotein P (SelP1) concentration when compared to the Na2SeO3 or control group. Compared with the control group, the antioxidant enzyme activity in the tissues from gilts in the HMSeBA group was increased, and the concentration of malondialdehyde in the colon had a decreasing trend (p = 0.07). Gilts in the HMSeBA supplemented group had upregulated gene expression of GPX2, GPX4, and SelX in spleen tissue, TrxR1 in thymus; GPX1 and SelX in duodenum, GPX3 and SEPHS2 in jejunum, and GPX1 in the ileum tissues (p < 0.05). In addition, compared with the control group, the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) in the liver, spleen, thymus, duodenum, ileum, and jejunum of gilts in the HMSeBA group were downregulated (p < 0.05), while the expression of interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) in the liver, thymus, jejunum, and ileum were upregulated (p < 0.05). Compared with the control group and the Na2SeO3 group, HMSeBA had increased concentration of serum cytokines interleukin-2 (IL-2) and immunoglobulin G (IgG; p < 0.05), increased concentration of intestinal immunoglobulin A (sIgA; p < 0.05), and decreased concentration of serum IL-6 (p < 0.05). Dietary supplementation with HMSeBA also increased the abundance of intestinal bacteria (Ruminococcaceae and Phascolarctobacterium; p < 0.05) and selectively inhibited the abundance of some bacteria (Parabacteroides and Prevotellaceae; p < 0.05). In short, HMSeBA improves the antioxidant performance and immune function of gilts, and changed the structure of the intestinal microflora. And this study provided data support for the application of HMSeBA in gilt and even pig production.

Metal determination and biochemical status of marine fishes facilitate the biomonitoring of marine pollution

In the present study, the bioaccumulation of chromium, manganese, cobalt, copper, zinc, selenium, arsenic, strontium, cadmium, tin, antimony and lead in tissues of thirty marine fish species collected from New Ferry Whorf, Sassoon dock and Versova fishing harbour in Mumbai, India, were analysed. The bioaccumulation patterns of these twelve elements were determined to assess pollution biomarkers based on cellular and oxidative stresses. Catalase, superoxide dismutase and glutathione-s-transferase, glycolytic enzymes viz. lactate dehydrogenase and malate dehydrogenase, protein metabolism enzymes viz. aspartate transferase and alanine transferase, and lipid peroxidation were significantly higher in muscle and gill tissues. The activities of the neurotransmitter enzyme acetylcholine esterase in muscle and brain tissues was inhibited due to pollution. This study suggested that biochemical attributes such as oxidative stress enzymes, cellular biomarkers, neurotransmitter enzymes and metal and metalloid contamination could be successfully employed, even at low concentrations, as reliable biomarkers for biomonitoring of contaminated marine ecosystems.

Association between trace element concentrations in cancerous and non-cancerous tissues with the risk of gastrointestinal cancers in Eastern Iran

This study was conducted to investigate the association between trace elements including cadmium (Cd), chromium (Cr), cobalt (Co), copper (Cu), iron (Fe), lead (Pb), nickel (Ni), selenium (Se), zinc (Zn), and arsenic (As) in gastrointestinal cancer tissue and non-cancerous tissue (suspected gastrointestinal cancer) in Eastern Iran. The samples of 63 gastrointestinal cancers (stomach (n = 20), esophageal (n = 19), and colorectal (n = 24) along with 63 controls in South Khorasan Province, Iran, were collected and analyzed using ICP-MS (Agilent 7900). Our results indicated that the concentrations of Co (1.3 ± 0.8, 1.3 ± 0.8 μg kg-1), Cr (8.1 ± 7.3, 11.0 ± 14.8 μg kg-1), Ni (29.0 ± 20.1, 39.5 ± 30.2 μg kg-1), Pb (6.9 ± 4.0, 6.1 ± 4.6 μg kg-1), and Zn (867.6 ± 159.1, 935.6 ± 196.2 μg kg-1) were significantly higher among esophagus and colon cancer cases than controls (p < 0.05). Similarly, stomach cancer cases showed higher Co, Cr, Ni, Se, and Zn and lower Cu concentrations than their controls (p < 0.05). Moreover, the Spearman correlation between metals revealed a mostly low to moderate correlation between metals. Our finding illustrated that the significant risk differences of Cr, Ni, Pb, Se, and Zn metals on esophagus cancer when considered the single predictor unadjusted for other metals and covariates RD (95% CI) - Cr: -0.274 (-0.463, -0.086), Ni: -0.288 (-0.457, -0.118), Pb: -0.171 (-0.463, -0.086), Se: -0.243 (-0.434, -0.051), and Zn: -0.094 (-0.143, -0.045) respectively. This study suggests that the trace element's exposure may be associated with gastrointestinal cancer risk. Additional studies are needed to elucidate the mechanisms underlying trace element carcinogenesis further.

The effect of the addition of nano selenium and vitamin E on productive performance and the characteristics of the physical and chemical carcass of broilers

This experiment was conducted in private poultry farms / Sharqat district during the period from 8/15/2020 to 19/9/2020 The aim of this study is to find out the effect of adding two levels of nano-selenium and securing E in the diet on productive, physiological and immunological performance in addition to the carcass’s physical and chemical characteristics. For meat broilers (Ross 308.) 234 birds of broiler meat were used in the experiment (Ross 308), one day old, not nationalized and equipped from the Mardin company hatchery / Erbil governorate and distributed randomly into 6) factors for each treatment (3) replicates of (13) birds for each Duplicate. The first treatment was the control treatment and the second treatment was a basic diet to which a supplement of nano-selenium was added at a concentration of 0.25 mg / kg, and the third treatment was a basic diet to which a supplement of nano-selenium was added at a concentration of 0.35 mg / kg, and the fourth treatment was a basic diet to which a supplement was added with a supplement with a vitamin E at a concentration of 300 mg / kg. The fifth and sixth are basic diets to which add nano-selenium at a concentration of 0.25 and 0.35 mg / kg + with insurance E at a concentration of 300 mg / kg, respectively. The results of the statistical analysis indicated that there were no significant differences in the production performance at this level of nano-selenium and vitamin E, while it showed The fifth treatment was significantly higher in the amount of feed consumed in the first and fourth weeks. As for the results of the statistical analysis of blood tests, it indicated that the number of red blood cells, the size of compact cells, and the hemoclopin ratio of the trial treatments improved significantly compared to the control treatment, while the white blood cells and lymphocytes showed significant differences (P ≤ 0.05) compared to the control treatment. As for the biochemical characteristics, we note that All treatments significantly outperformed (P ≤ 0.05) over the control treatment, but no significant differences were observed between the experimental treatments in the characteristic of low-density lipoproteins. As for the physical properties of the meat, there were significant differences (P ≤ 0.05) in favor of the nano-selenium treatments, where the sixth treatment recorded the highest concentration of selenium in the breast meat tissue, while the second, fourth and fifth treatments recorded a significant decrease (P ≤ 0.05) in the pH of the meat compared with the control treatment. This reduces the microbial activity and prolongs the storage and preservation period of meat, and in the chemical properties the results indicated the presence of significant differences (P ≤ 0.05) in the percentage of protein, moisture, fat and ash, and therefore our results showed that the use of nano-selenium and vitamin E led to the improvement of most of the biochemical and immunological properties of blood, in addition to To improve the physical and chemical properties of broiler carcasses.