The present study shows that copper(II) ions can be determined with a new fluorescent probe that is based on the use of CdSe quantum dots capped with deep eutectic solvent (DES-CdSe QDs). The capped QDs were prepared in aqueous phase by a one-step procedure under ambient atmosphere using selenium dioxide as a stable precursor for selenium, and ascorbic acid as non-toxic reducing agent. The deep eutectic solvent is composed of choline chloride and thioglycolic acid and acts as stabilizing and functionalizing agent. The fluorescent probe undergoes an increase in the fluorescence intensity (with excitation/emission wavelengths at 380/560nm) in the presence of Cu(II). Other ions display no significant effect on fluorescence. The effects of sample pH value, concentration of buffer, and volume of QDs solution were optimized by response surface methodology using a Box-Behnken statistical design. Under the optimal conditions, the response of the probe is linear in the 10-600nM Cu(II) concentration range, with a 5.3nM limit of detection. This is lower than the allowable maximum Cu(II) concentration in drinking water. The relative standard deviation of the method for five replicate measurements of Cu(II) at a 100nM concentration level is 2.0%. The probe was successfully applied to the determination of Cu(II) in various drinks. Graphical abstractSchematic representation of a fluorometric method for the determination of Cu(II) at nanomolar concentration levels. The fluorescent system consists of deep eutectic solvent-capped cadmium selenide quantum dots (DES-CdSe QDs). Fluorescence is strongly enhanced by copper(II).