The growing health risks linked to environmental exposure to mutagenic, teratogenic, and carcinogenic herbicides have sparked a significant interest in developing rapid and cost-effective detection technologies for accurate quantification of these compounds. Therefore, current research concerns a selective and sensitive electrochemical determination of Aminotriazole (AMT) using an inexpensive modified polymerized methyl orange pencil graphite electrode (PMO/PGE). The PMO/PGE and bare pencil graphite electrode (BPGE) have been characterized through various analytical techniques such as scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX) and electrochemical impedance spectroscopy (EIS). The electro-oxidation behavior of AMT was investigated by utilizing various methodologies including cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV) in 0.1 M phosphate buffer solution (PBS) of pH 7.0. AMT exhibits a significant increase in anodic peak current of 7.15 µA at PMO/PGE compared to BPGE. The effect of electro-kinetic factors involved in the electro-oxidation of AMT, such as the heterogeneous rate constant, scan rate, surface concentration, pH and the number of electrons and protons transferred were resolved. The electro-oxidation reaction involves two protons and two electrons, according to the electrochemical analysis of AMT at PMO/PGE. The number of electrons involved in a redox reaction is often determined experimentally, and it is a key parameter in understanding the reaction mechanism. Under the investigational conditions, the anodic peak current of the analyte is directly proportional to its concentration in the range 1.0 – 13.0 µM with the limit of detection (LOD) being 1.24 × 10−8 M. The developed modifiedsensor has got good reproducibility, sensitivity, selectivity and stability, and it could be successfully validated for the detection of AMT present in real samples.