Karo Regency as the largest corn production centre in North Sumatra Province is at risk of having a high frequency of paraquat-resistant populations of Eleusine indica, this is due to the continuous use of paraquat for a long time. This study aims to determine the distribution and frequency paraquat-resistant population of E. indica on cornfield in Karo Regency. Sampling was conducted in March - July 2019. Weed seed samples were taken from six Sub-districts that have the largest corn production in Karo Regency. Resistance tests were conducted in August - September 2019 at the weed resistance research laboratory in the Faculty of Agriculture, Universitas Sumatera Utara. Weed seeds from 72 suspected paraquat-resistant populations and 1 paraquat-sensitive population were planted each with 10 plants / pots and arranged using a Randomized Block Design in four replications. The paraquat dose used is the recommended dose of 600 g.b.a./ha. The application is carried out using an electric knapsack sprayer with teejetnozle and a spray volume of 320 L per hectare. The results of this study indicate that there are 70 populations (97.2%) of the paraquat-resistant category, 1 sample (1.4%) of the paraquat-resistant developing category and 1 population (1.4%) of the paraquat-sensitive category.