Propagation Of Seedlings Research Articles

Physiological Indexes in Seed Germination and Seedling Growth of Rangpur Lime (Citrus limonia L. Osbeck) under Plant Growth Regulators

The propagation of citrus seedlings is accomplished through grafting, utilizing seeds for the production of rootstocks. The germination of certain seeds may be low and uneven, complicating the production of high-quality seedlings. The use of plant growth regulators (PGRs) is a viable alternative to improve the quality of seedling production, as these compounds can break dormancy, control the hydrolysis of reserves, induce cell division, and regulate permeability and protein functions. This study aimed to evaluate the germination of seeds and the growth of Rangpur lime (Citrus limonia L. Osbeck) seedlings under the influence of imbibition in solutions of gibberellic acid (GA3) and a combination of GA4+7 + 6Benzyladenine. The experiment was conducted under controlled laboratory and greenhouse conditions, using a completely randomized design in a 2 × 5 factorial scheme, with two types of plant regulators (GA3 and GA4+7 + 6BA) at five concentrations (0, 250, 500, 750, and 1000 mg L−1 a.i.). Quantitative and qualitative variables were evaluated, ranging from seed germination to seedling development and formation, including germination percentage and speed index, fresh and dry biomass of roots and shoots, enzymatic activity, and gas exchange. The results indicate that GA3 significantly accelerates the germination process of Rangpur lime cv. Santa Cruz seeds and promotes better seedling growth and development, resulting in vigorous seedlings. These findings demonstrate that the application of PGRs, particularly GA3, can substantially enhance the propagation efficiency of citrus rootstocks, offering a practical solution for improving the uniformity and quality of seedling production in commercial settings.

Effects of steel slag mixed substrate on rooting of Hydrangea macrophylla cuttings.

To investigate the effect of steel slag used as substrate on the rooting of Hydrangea macrophylla cuttings, and to develop a new mixed substrate that can partially replace conventional cutting substrates and realize the high-efficient utilization of solid waste, we examined the physical and chemical properties of different mixed substrates containing 10% (T1), 20% (T2), 30% (T3), and 40% (T4) volume fractions of steel slag, and investigated the rooting of H. macrophylla 'Red Beauty' cuttings growing on these substrates, with conventional cutting substrates (peat and perlite) as the control (CK). The results showed that pH value, electrical conductivity, and bulk density of the mixed substrates were significantly higher than those of CK. The aeration porosity of T2 was higher than other treatments, while the total porosity and water holding porosity differed little from others. Both fresh weight and dry weight of all the four treatments were higher than those of CK, with stem diameter being higher than that of CK (except T4), plant height showing no significant difference compared to CK (except T4), and leaf chlorophyll content being significantly lower than CK. Root length ranked as T2>CK>T1>T3>T4, the root surface area and root volume both ranked as T2>T1>CK>T4>T3, the root tip ranked as T2>CK>T1>T4>T3. Both average root diameter and root activity were significantly higher than that of CK, with the highest value being observed in T2. Soluble sugar content in the leaves of T2 was the highest, followed by T4, T3, CK, and T1. The weight ranking of root growth indices was root activity > average root diameter > root volume > root surface area > root tip number > root length. Redundancy analysis indicated that pH value, electrical conductivity, aeration porosity, and water holding porosity of substrates were key factors influencing root growth and development of cuttings. Our results suggested that substrates mixed with 10% to 40% steel slag could be used for H. macrophylla cutting propagation, and 20% (T2) being the best one because it could significantly improve the survival rate, growth status, and root development of cuttings. Steel slag would be a novel substrate to partially replace conventional unrenewable substrates such as peat and perlite for flower seedling propagation, which could reduce agricultural production cost and provide a high-value utilization way of industry solid waste.

Induction of Somatic Embryogenesis of Pontianak Siamese Orange Cotyledon Cultures on Murashige Skoog Media with the Addition of 2,4-D and Kinetin

Conventional citrus propagation has weaknesses, one of which is susceptible to CVPD(Citrus Vein Phloem Degeneration) and other diseases which are the main cause of the decline in population and production of pontianak siam orange plants in West Kalimantan. One alternative to in vitro propagation of citrus seedlings is the induction of somatic embryogenesis. The purpose of the study was to obtain the best combination of 2,4-D and kinetin concentrations for callus induction and somatic embryo induction from Pontianak siam orange cotyledon explants, observing the developmental phase (stage) of embryos formed. The implementation of the study used a factorial completely randomized design (CRD) consisting of 2 factors. The first factor is the concentration of 2,4-D consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L. The second factor is kinetin concentration consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L.The observation parameters include the time of callus emergence (hst), the percentage of explants forming callus (%), the texture and color of the callus, observing the stage of the embryo found. The results showed that 72% of treatments were able to induce callus from pontianak siam orange cotyledons with an average callus appearance at 7 hst. The callus formed is compact and crumbly with varied callus colors, namely white, yellowish, greenish and brownish. The best treatment that produces embryogenic callus is the combination of D0.5K0.5; D1K1; D0.5K1.5; D1.5K1.5 and D2K2 with the characteristics of crumbly callus and yellowish and brownish colors. The results of microscopic observations found that the somatic embryo phase formed was the pro-embryo phase.

Practical Guidelines for Farm Waste Utilization in Sustainable Kale Production

Natural amendments from agricultural waste to improve soil physicochemical properties continuously attract research interest in promoting eco-friendly plant production. The present study evaluated the proper use of sawdust, biochar, and compost made from farm waste for kale production from seedling propagation to field conditions. From the seedling propagation process, the results demonstrate that the most suitable growing medium for kale seedings was 0.5:1:1 v/v of sawdust + biochar + compost, which gave the fastest mean germination times (2.71 days) and the highest seed germination percentage (78.33%). In addition to investigating the selected growing media as the soil amendments at five different rates (0, 6.25, 12.50, 18.75, 25.00, and 31.25 t ha−1), the result reveals that the fresh weight of marketable leaves was significantly highest under the 31.25 t ha−1 treatment. The application rate that yielded the highest gross profit margins was eight times higher than the control. Moreover, in some harvesting periods, the kale leaf yields under the treatment of 31.25 t ha−1 showed higher total chlorophyll and carotenoid contents.

Establishment of Tissue Culture and Regeneration System in Hippophae gyantsensis Lian

Hippophae gyantsensis Lian is a pioneering tree species in Chinese forestry ecological engineering, known for its robust stress tolerance, water retention capacity, and soil improvement qualities. However, the lack of rapid nursery technology has been a significant impediment to the development of the H. gyantsensis industry. In the present study, we have successfully established a tissue culture regeneration system for H. gyantsensis. The most effective methods for seed disinfection, ensuring sterility in seedlings, were found to be 75% alcohol disinfection for 40 s and 10% sodium hypochlorite disinfection for 10 min. The best media tested for callus induction in cotyledons and hypocotyls of sterile seedlings were 1/3 MS + 0.5 mg/L KT + 0.75 mg/L NAA and 1/3 MS + 0.3 mg/L 6-BA + 1.5 mg/L IBA, respectively. As the explants, cotyledons yielded larger calli with a greater size and differentiation ability than hypocotyls. For the induction of indeterminate shoots and proliferation, the most suitable media were 1/3 MS + 0.5 mg/L IAA + 0.75 mg/L 6-BA and 1/3 MS + 1.0 mg/L 6-BA + 0.05 mg/L IBA + 0.1 mg/L KT, respectively. Lastly, the best worked rooting formulation was 1/4 MS + 0.3 mg/L IBA. This study marks a significant milestone in the establishment of a systematic tissue culture regeneration system for H. gyantsensis, which will facilitate the industrial rapid propagation of high-quality seedlings and provide the foundation for improvement through genetic transformation.

Substrate Comparison for Tomato Propagation under Different Fertigation Protocols

Greenhouse tomato production faces multiple challenges, including the excessive use of nonrenewable substrates that are difficult to dispose of after use. Currently, most growers propagate tomatoes in rockwool, but there is an increasing demand for sustainable media. The objective of this research was to evaluate sustainable and organic alternatives for greenhouse propagation of tomato seedlings intended for high-wire production. Different organic and inorganic substrates were evaluated in three experiments, using a nutrient solution composed of a complete water-soluble fertilizer. Germination and growth parameters, including height, stem diameter, number of leaves, leaf area, foliar chlorophyll levels (SPAD), and shoot fresh and dry weight, were measured. In the first experiment, which employed overhead irrigation, rockwool, coir, wood fiber–coir mix, medium-grade pine bark, pine bark < 0.64 cm, and pine bark < 0.32 cm were evaluated. Tomato germination was faster and achieved higher percentages with pine bark < 0.64 cm compared to other substrates. However, growth performance was similar or better in coir than in rockwool four weeks after transplantation. For the second experiment with sub-irrigation only, rockwool, coir, wood fiber–coir mix, pine bark < 0.32 cm bark, and peat were evaluated at different container heights. Peat resulted in greater growth across all parameters, followed by wood fiber–coir mix in all container heights, while pine bark had the least growth across all measured parameters. In the third experiment with overhead irrigation, rockwool, wood fiber–coir mix, pine bark < 0.32 cm, and a commercial peat-based mixture were evaluated under different fertilizer rates (electrical conductivity of 1.1 and 2.2 mS·cm−1). Wood fiber–coir mix, peat-based mix, and rockwool were the substrates with the highest values for all evaluated parameters. While all the organic substrates showed potential for use in tomato propagation, pine bark < 0.32 cm bark and wood fiber–coir mix provided the best media for germination. Peat and wood fiber–coir mix showed the best media for subsequent seedling growth and demonstrated potential to be used as substitutes for rockwool.

Enraizamento de estacas de goiabeira ‘Pedro Sato’ em função do diâmetro, tipos de cortes e tratamento com bioestimulante

Abstract: For the propagation of guava seedlings to occur, it is necessary to address numerous factors, therefore, the objective was to verify the influence of the diameter and type of cut on the base of ‘Pedro Sato’ guava tree cuttings. Experiment I: implemented in a 3 × 5 factorial scheme, consisting of three types of cut at the base of the cutting and five biostimulant concentrations (0, 5, 10, 15 and 20 g L-¹). Experiment II: implemented in a 2 × 5 factorial scheme, composed of two stem diameters (D1: 3 - 3,99 mm; D2: 4 - 4,99 mm) combined with five biostimulant concentrations (0, 5, 10, 15 and 20 g L-¹), and both experiments were conducted in a completely randomized design, with 4 replicates of 10 cuttings per plot. Considering the above, it could be concluded that the use of biostimulant increases the rooting rate. Cut with the removal of lateral portions at the base of the cutting promotes greater mortality, while evidencing less increase in root length. In experiment II, it was found that cuttings with diameter between 4 and 4.99 mm show greater survival rate at concentrations of 0 and 5 g L-¹, while cuttings with diameter between 3 and 3.99 mm have higher rooting rate at concentrations 0 and 5 g L-¹ but with high mortality rate at concentration of 5 g L-¹.

Application of innovative technologies in the propagation of mulberry seedlings from cuttings in closed areas and use in the organization of maternity intensive mulberry groves

Application of innovative technologies in the propagation of mulberry seedlings from cuttings in closed areas and use in the organization of maternity intensive mulberry groves

Disease of forest tree seedlings (Article Review)

Nursery are one of the most important reasons for the success and progress of the agricultural renaissance, as it depends on the application of various advanced scientific methods and the use of Nursery of various types in the propagation and production of forest seedlings and others. A greenhouse is a place where small plants are produced to plant them in gardens, fields, and trees. The plants produced by the greenhouse vary between ornamental plants and flowers, fruit plants, vegetables, and forest trees, and Nursery are often held for commercial purposes.The incidence of Nursery diseases increases from what happens in the forests through the increase in service operations and attention to irrigation and fertilization and not sterilizing the soil and seeds, as this appears on the resulting seedlings, and the seedlings resulting from Nursery can be a source of transmission of infection when artificial afforestation is performed. Therefore, we must monitor and ensure the safety of the productive seedlings. The disease of Root rot and seedling disease Damping-off are one of the most critical diseases found in forest Nursery and the most widespread throughout the hot and cold world. As these diseases affect many forest plants, it is preferable to study them to avoid infecting forest species through the correct management of these Nursery by sterilizing the soil and seeds, as well as regular irrigation and correct fertilization.

Symbiotic seed germination and seedling growth of mycorrhizal fungi in Paphiopedilum hirsutissimun (Lindl.Ex Hook.) Stein from China

ABSTRACT Similar to other orchid species, Paphiopedilum hirsutissimum (Lindl.ex Hook.) Stein, relies on nutrients provided by mycorrhizal fungus for seed germination and seedling development in the wild owing to a lack of endosperm in its seeds. Therefore, obtaining suitable and specialized fungi to enhance seed germination, seedling formation, and further development is considered a powerful tool for orchid seedling propagation, reintroduction, and species conservation. In this study, we investigated the diversity, abundance, and frequency of endophytic fungal strains in the root organs of P. hirsutissimum. One family and five genera of the fungi were isolated and identified through rDNA-ITS sequencing. The ability of isolated fungi to germinate in vitro from the seeds of this species was evaluated, and the development of P. hirsutissimum protocorm has been described. The findings showed that the treatments inoculated with endophytic fungal DYXY033 may successfully support the advanced developmental stage of seedlings up to stage 5. In addition, scanning electron microscopy (SEM) revealed that the mycelium of this strain began to invade from either end of the seeds up to the embryo, extending rapidly from the inside to the outside. Its lengthening resulted in the bursting of the seed coat to form protocorms, which developed into seedlings. The results showed that DYXY033 has a high degree of mycobiont specificity under in vitro symbiotic seed germination conditions and is a representative mycorrhizal fungus with ecological value for the species. In summary, this strain may particularly be significant for the protection of P. hirsutissimum species that are endangered in China. In the long run, it may also contribute to global efforts in reintroducing orchid species and in realizing in situ restorations of threatened orchid populations.

Growth and quality of Geronggang (Cratoxylum arborescens) seedling in different types of growing mediums and containers

Geronggang (Cratoxylum arborescens) is one of native tree species of tropical peat swamp forest which has a good potency both planted for rehabilitation and industrial purposes. Nevertheless, before this tree species can be cultivated on a large scale, much silviculture knowledge and technology must be accessible, including the technique of seedling propagation. This study evaluated the growth and quality of geronggang seedlings grown in different mediums and containers. The seedling was grown in topsoil (M1) and peat (M2) medium with three containers (C1 = plastic pot (volume (v) = 120 mL); C2 = plastic pot (v = 300 mL) and C3 = polybag (v = 400 mL)). As the control, the seedling was also grown in topsoil + compost medium combined with polybag (M3C3). Growth (height (H), collar diameter (D), and biomass) and morphological ((shoot/root (S/R), D/H) and Dickson quality index (DQI)) attributes of the seedling and medium properties were observed. Peat medium had better results than topsoil (p < 0.05). Furthermore, the seedlings grown in peat medium had the highest performance. M2W3 was the treatment with adequate quality to be outplanted at nine months, with H, D, S/R, H/D, and DQI were 25.4 cm, 3.6 mm, 6.94, 1.37, and 0.36, respectively. The results indicated that using peat alone as a medium in geronggang seedling propagation was relatively time-consuming.

Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times

Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.

Survival of five diazotrophic bacterial strains in different substrates used in sugarcane seedling propagation.

An inoculant containing selected bacterial strains can be easily applied during the nursery please process, but in this case, substrate composition can affect its survival and, in consequence, bacterial colonization. The aim of this study was to evaluate the survival of five diazotrophic bacterial strains/species applied individually on 13 different substrates used in sugarcane seedling nurseries considering an active population higher than 105 cells g-1. In addition, one experiment was performed using two commercial substrates, coconut fiber, and Multiplant™, inoculated or not with a mixture of five bacterial strains to evaluate plant growth after 30days. These strains are combined inoculants selected for sugarcane acting as plant growth promoters. Bacterial counts were determined every seven days using the Most Probable Number technique with four different semi-solid N-free media specific for each strain tested over 35days. The survival order, independent of the substrate tested, was: Paraburkholderia tropica Pt-PPe8T > Nitrospirillum amazonense Na-CBAMc > Herbaspirillum seropedicae Hs-HRC54 = H. rubrisubalbicans Hr-HCC103 > Gluconacetobacter diazotrophicus Gd-PAL5T. All tested substrates influenced the bacterial survival, especially after 21days of incubation. The population size can be partially controlled by the substrate pH and stimulated by the addition of slow-release fertilizer. Besides the differences in the bacterial population present in the two commercial substrates, plant growth was found to be stimulated by the inoculated bacteria, depending on the substrate and its sugarcane cultivar tested. The selection of a substrate used to produce new plantlets of sugarcane can contribute to bacterial survival and improve bacterial colonization.

The Effect of Hormone Types, Concentrations, and Treatment Times on the Rooting Traits of Morus 'Yueshenda 10' Softwood Cuttings.

Enhancing the capacity of fruit trees to propagate via cuttings is an important endeavor for the high-quality development of the fruit industry. Optimizing the conditions for the cutting propagation of mulberry seedlings is an important factor that influences the industrial production of this plant; however, the currently used mulberry breeding technology system is not mature. In this experiment, an orthogonal design was used to intercept semi-woody shoots of Yueshenda 10 as cuttings and set different hormone concentrations (200, 500, 800, and 1000 mg/L), different hormone types (NAA, IBA, IAA, and ABT-1), and different soaking times (10, 30, 60, and 120 min) for cuttings. The effects of the three factors on the rooting of mulberry cuttings were investigated by soaking the cuttings in clean water for 10 min as a control. The results showed that the primary and secondary order of the three factors affecting the rooting rate of cuttings was hormone concentration > hormone type > soaking time, and the concentration of exogenous hormones had a significant impact on all rooting indicators (p < 0.05). In addition, the rooting rate (66.24%), average number of roots (7.54 roots/plant), and rooting effect index (4.23) of Yueshenda 10 cuttings reached the optimal level when soaked with 800 mg/L ABT-1 for 30 min. The longest root length (10.20 cm) and average root length (4.44 cm) of cuttings achieved the best results when soaked with 800 mg/L NAA for 60 min and 500 mg/L NAA for 30 min, respectively. On balance, it is considered that the preferred solution is to soak the cuttings of Yueshenda 10 with 800 mg/L ABT1 solution for 0.5 h.

Environmental life cycle assessment of production of the high intensity sweetener steviol glycosides from Stevia rebaudiana leaf grown in Europe: The SWEET project

PurposeThere is an increasing interest in the use of non-nutritive sweeteners to replace added sugar in food and beverage products for reasons of improving consumer health. Much work has been done to understand safety of sweeteners, but very little on sustainability. To address that gap, this study presents the results of a life cycle assessment (LCA) of production of rebaudioside A 60%, 95% pure (RA60) steviol glycoside mix from Stevia rebaudiana leaf grown in Europe.MethodsAn attributional cradle-to-factory-gate life cycle assessment was conducted on growing of stevia leaves and extraction of steviol glycosides in Europe. Primary data were used from a case study supply chain. Results are reported in impact categories from the ReCiPe 2016 (H) method, with focus given to global warming potential, freshwater eutrophication, water consumption, and land use. Impacts are expressed both in terms of production mass and sweetness equivalence, a common metric for understanding high intensity sweetener potency. Sweetness equivalence of RA60 is typically 200 to 300 times that of sugar. Comparison of environmental impact is made to sugar (sucrose) produced from both cane and beets. The research is part of the EU project SWEET (sweeteners and sweetness enhancers: impact on health, obesity, safety, and sustainability).Results and discussionGlobal warming potential for production of RA60 was found to be 20.25 kgCO2-eq/kgRA60 on a mass basis and 0.081 kgCO2-eq/kgSE on a sweetness equivalence basis. Field production of stevia leaves was found to be the main source of impact for most impact categories, and for all four focus categories. Extraction of the RA60 was the main source of impact for the others. Leaf processing and seedling propagation were minor contributors to life cycle impact. Removal of international transport from the supply chain reduced global warming potential by 18.8%. Compared with sugar on a sweetness equivalence basis, RA60 has approximately 5.7% to 10.2% the impact for global warming potential, 5.6% to 7.2% the impact for land use, and is lower across most other impact categories.ConclusionThis is the first LCA of steviol glycoside mix RA60 produced from leaf in Europe. The results indicate that RA60 can be used to reduce environmental impact of providing a sweet taste by replacing sugar across all impact categories. However, it is important to note that specific formulations in which RA60 is used will have a bearing on the final environmental impact of any food or beverage products. For solid foods, this requires further research.

Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Alocasia of Indonesian endemic has significant market potential. However, the scarcity of qualified seedlings hampered their commercialization. The purpose of this study was to obtain the best sterilization technique and media for in vitro propagation of Alocasia cuprea through the organogenesis pathway using microbulblet explants. Randomized complete block designs with five to ten replicates were applied in the experiments. Application of 5 and 10% clorox for 5 and 10 minutes resulted in 40.6% aseptic explants, 10% browning explants, and 90% explant growth potential. The best shoot initiation was achieved on the MS added 4.54 μM thidiazuron and 2.22 μM BAP. This medium had an 85% explant initiation, 4.2 shoots per explant, and 6.5 days initiation time. The best shoot proliferation was discovered on MS medium with 4.44 μM BAP, which produced 10.5 initial shoots with a shoot height of 1.3 cm and a clump diameter of 1.3 cm. The best plantlet regeneration was determined on ½ MS medium with 6.5 cm plantlet height, 3.5 leaves total, 3.8 cm leaf length, 2.6 cm leaf width, and 3.8 roots. This propagation method will be applicable to the propagation of qualified seedlings for commercial purposes and potentially used for other endemic Alocasia.

Combined Effects of Scarification, Phytohormones, Stratification, and Soil Type on the Germination and/or Seedling Performance of Three Tamaulipan Thornscrub Forest Species.

Tamaulipan thornforests in south Texas and northeast Mexico are a conservation hotspot. Shortages of native seedlings limit regional restoration and are largely driven by knowledge gaps regarding propagation of the 75+ thornforest species planted during restorations. We previously investigated three thornforest species with low or inconsistent germination or seedling survival: Ebenopsis ebano (Fabaceae), Cordia boissieri (Boraginaceae), and Zanthoxylum fagara (Rutaceae), and identified the types and dosages of chemical seed treatments that maximized germination. However, chemical treatments were performed in isolation and combinational treatments may be required to break dormancy or maximize germination. This study builds on prior work by investigating the effects of all possible combinations of sulfuric acid (SA), gibberellic acid (GA), and indole-3-butyric acid (IBA) treatments on germination of the same focal species, and further quantified the combined effects of five chemical treatments, three stratification treatments, and six soil mixture types on the germination and seedling performance of the focal species. Ebenopsis ebano germination peaked with SA and was not improved with additional chemical treatments. Cordia boissieri germination was highest with GA only in our indoor experiment but peaked with GA + IBA + SA in our outdoor experiment. Zanthoxylum fagara germination was near zero in all treatments. Stratification treatments marginally reduced E. ebano germination and reduced C. boissieri seedling height. Soil type had significant impacts on E. ebano germination and leaf abundance (residual differences up to 40% or 4 leaves, respectively) and influenced some of the effects of chemical treatments. These results enhance our understanding of thornforest seed ecology and best practices for nursery propagation of seedlings.

In vitro regeneration of Senna alata (L.) Robx. (Fabaceae): An ancient medicinal plant

Senna alata, a legume of medicinal importance, presents antifungal and antibacterial antioxidant activity. The main limitation is the presence of seeds with integument dormancy within natural populations. This study aimed to establish an efficient protocol for the in vitro propagation and acclimatization of seedlings. The seeds were disinfected with different concentrations of NaOCl; for the regeneration of the shoots, cotyledon segments were inoculated in MS and WPM medium, supplemented with plant growth regulators, auxins (1-NAA and 2,4-D), interaction with cytokinins (TDZ and BAP), and activated carbon. The seedlings were acclimated in nursery conditions using vermiculite, sand, and soil substrates. Using NaOCl (0.5%) for 30 min resulted in 98.0% disinfected seeds while 95.4% of scarified seeds germinated. High node and shoots numbers (8.3 ± 0.4; 12.6 ± 0.8, respectively) were obtained when using the MS medium containing 1-NAA + TDZ (0.1 + 1.0 mg L-1), while the use of MS medium supplemented with 2,4-D + TDZ (0.1 + 1.0 mg L-1) resulted in longer shoots (6.0 ± 0.5 cm). Using WPM medium with IBA + BAP (1.0 + 0.1 mg L-1) improved rooting (14.0 cm ± 1.3) and the number of roots (5.0 ± 0.0) in the treated plants. The vermiculite substrate with sand promoted 81.5% of acclimatized plants. This study allowed obtaining high levels of germination, regeneration in vitro and acclimatization of S. alata.

Genetic studies in mulberry seedlings by diallel analysis

Genus Morus includes more than 20 species, some of which are commercially important mulberries with different utilities. Even though these species are propagated by rooting of cuttings in practice, mulberry seeds are of importance for breeding studies and rootstock seedling propagation. For that reason, this study was conducted to know the seed performance for germination and emergence traits of four mulberry varieties viz, V1, G4, S36, MR2 using 4× 4 complete diallel mating design. According to the results both non – additive and additive gene actions are important in the inheritance of these traits. Progenitors MR2 and V1 were found to be the best combiners for most of the traits studied.

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Toboali pineapple variety is a smooth cayenne variety because it contains high antioxidants in the form of vitamins A and C. In addition, it is a source of citric and malic acids, substances that can be used to enhance the taste of the fruit. Pineapple is a plant that is propagated with vegetative organs such as suckers, slips, and crowns. However, conventional propagation has a low propagation rate. Tissue culture is an in vitro technique used in the rapid propagation of plants and in large quantities to meet the needs of cultivation and research. The selection of regeneration media is very influential on the development of plants in tissue culture. This study aimed to obtain the optimal media combination with BAP and NAA in the regeneration of Smooth Cayenne pineapple (Toboali) in vitro for mass propagation of seedlings and genetic engineering research. This study used 6 levels of BAP, namely 0, 1, 2, 3, 4, 5 mg.L&lt;sup&gt;-1&lt;/sup&gt; and 4 levels of NAA, namely 0, 1, 2, 3 mg.L&lt;sup&gt;-1&lt;/sup&gt;. The treatment used was a combination of BAP and NAA as many as 24 treatments with 3 replications. The best pineapple regeneration medium for mass plant propagation in this study was BAP 3 mg.L&lt;sup&gt;-1&lt;/sup&gt; without NAA media which produced the highest shoots of 17.33 shoots per explant at 12 week after planting. The best pineapple regeneration medium for genetic engineering research was a combination BAP 3 mg.L&lt;sup&gt;-1&lt;/sup&gt; and NAA 2 mg.L&lt;sup&gt;-1&lt;/sup&gt; media which produced 18.33 shoots per explant from embryogenic callus.