Secondary Defects Research Articles

CADASIL mutant NOTCH3(R90C) decreases the viability of HS683 oligodendrocytes via apoptosis.

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common hereditary cerebral small vessel disease caused by mutations in NOTCH3. Prevailing models suggest that demyelination occurs secondary to vascular pathology. However, in zebrafish, NOTCH3 is also expressed in mature oligodendrocytes. Thus, we hypothesized that in addition to vascular defects, mutant NOTCH3 may alter glial function in individuals with CADASIL. The aim of this study was to characterize the direct effects of a mutant NOTCH3 protein in HS683 oligodendrocytes. HS683 oligodendrocytes transfected with wild-type NOTCH3, mutant NOTCH3(R90C), and empty control vector were used to study the impact of the NOTCH3(R90C) mutant on its protein hydrolytic processing, cell viability, apoptosis, autophagy, oxidative stress, and the related upstream events using immunoblotting, immunofluorescence, RT-PCR, and flow cytometry. We determined that HS683 oligodendrocytes transfected with mutant NOTCH3(R90C), which is the hotspot mutation site-associated with CADASIL, exhibited aberrant NOTCH3 proteolytic processing. Compared to cells overexpressing wild-type NOTCH3, cells overexpressing NOTCH3(R90C) were less viable and had a higher rate of apoptosis. Immunoblotting revealed that cells transfected with NOTCH3(R90C) had higher levels of intrinsic mitochondrial apoptosis, extrinsic death receptor path-related apoptosis, and autophagy compared with cells transfected with wild-type NOTCH3. This study suggests that in patients with CADASIL, early defects in glia influenced by NOTCH3(R90C) may directly contribute to white matter pathology in addition to secondary vascular defects. This study provides a potential therapeutic target for the future treatment of CADASIL.

Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice

Sanfilippo syndrome type B (mucopolysaccharidosis IIIB), caused by inherited deficiency of α-N-acetylglucosaminidase (NAGLU), required for lysosomal degradation of heparan sulfate (HS), is a pediatric neurodegenerative disorder with no approved treatment. Intracerebroventricular (ICV) delivery of a modified recombinant NAGLU, consisting of human NAGLU fused with insulin-like growth factor 2 (IGF2) for enhanced lysosomal targeting, was previously shown to result in marked enzyme uptake and clearance of HS storage in the Naglu−/− mouse brain. To further evaluate regional, cell type-specific, and dose-dependent biodistribution of NAGLU-IGF2 (BMN 250) and its effects on biochemical and histological pathology, Naglu−/− mice were treated with 1–100 μg ICV doses (four times over 2 weeks). 1 day after the last dose, BMN 250 (100 μg doses) resulted in above-normal NAGLU activity levels, broad biodistribution, and uptake in all cell types, with NAGLU predominantly localized to neurons in the Naglu−/− mouse brain. This led to complete clearance of disease-specific HS and reduction of secondary lysosomal defects and neuropathology across various brain regions lasting for at least 28 days after the last dose. The substantial brain uptake of NAGLU attainable by this highest ICV dosage was required for nearly complete attenuation of disease-driven storage accumulations and neuropathology throughout the Naglu−/− mouse brain.

Effect of irradiation with 15-MeV protons on the compensation of Ge〈Sb〉 conductivity

The processes of the compensation of n-type conductivity in germanium irradiated with 15-MeV protons are investigated. Irradiation results in a considerable reduction in the density of shallow donor states of Group-V atoms. The rate of removal of shallow donor states due to the interaction between impurity atoms and radiation-induced intrinsic point defects is ~215 cm–1. The majority of secondary defects produced under proton irradiation are electrically neutral in an n-type material. Radiation-induced acceptors are of little importance in this case. Numerical modeling is performed, and the distribution of the energy transferred to recoil atoms is obtained. Two energy intervals are considered in the analysis of distribution histograms. At low energies, individual Frenkel pairs with closely spaced components are produced. The energy of recoil atoms in the second energy region is sufficient to induce a displacement cascade. Nanoscopic regions with high densities of intrinsic point defects and their complexes with dopant atoms are formed in such cascades. A model of the generation of intrinsic defects in germanium under proton irradiation is discussed.

Two-dimensional electrophoresis highlights haptoglobin beta chain as an additional biomarker of congenital disorders of glycosylation

Congenital disorders of glycosylation (CDGs) are rare inherited disorders affecting glycosylation of proteins and lipids and sharing very heterogeneous multivisceral symptoms. The biochemical screening of these diseases is currently limited to electrophoresis or HPLC separation/quantification of serum transferrin glycoforms and is relatively frequently hampered by genetic polymorphism. Further, it has been shown that transferrin glycosylation can be very poorly affected in confirmed CDGs. We developed a fast and simple two-dimensional (2-DE) Western-blot analysis applied to the simultaneous detection of various serum glycoproteins, i.e. haptoglobin, α1-anti-trypsin, transferrin and α1-acid glycoprotein, and applied it to a large cohort of CDGs and secondary glycosylation disorders. When separated using 2-DE, haptoglobin β glycoforms showed clear abnormalities in all interpretable CDG type I and CDG type II patterns. Although secondary glycosylation defects such as alcoholism, untreated fructosemia and bacterial neuraminidase remain to be excluded, we showed that 2-DE pattern of haptoglobin β glycoforms thus constitute a very reliable additional biomarker of all types of CDGs. Coupled with common screening techniques and glycans mass spectrometry, it can orientate and facilitate the way towards CDG molecular diagnostic.

Nasal Reconstruction and Repair of Secondary Nasal Deformities Following Treatment of Nasal Hemangiomas

Secondary nasal deformities and retardation of development due to treatment of nasal hemangioma during infancy are a challenge when it comes to nasal reconstruction. In order to evaluate nasal repair and reconstruction in these patients, the authors compared the ease and outcomes of using expanded forehead, nasolabial sulcus, and medial upper arm tube flaps. According to the deformities and patients' wishes, flaps were selected; using autogeneic rib cartilage, auricle cartilage, or silica gel as a scaffold or without framework; the inner lining were made by the residual scar tissue or the distal end of transferred flap. The esthetical and functional scores were recorded by the Nasal Appearance and Function Evaluation Questionnaire score to evaluate the effectiveness of the methods. From January 2010 to December 2015, 34 patients were included. Postoperative follow-up went for 12 to 36 months. The expanded forehead flap was used in 28 patients, the nasolabial sulcus flap in 5 patients, and the medial upper arm tube flap in 1 patient. Regarding framework, 20 patients used rib cartilage, 8 patients used auricle cartilage, 1 patient used silicone, and 5 patients did not use any framework. All patients reported the increasing nasal appearance and function evaluation. Repair of secondary nasal defects following treatment of hemangiomas in infants and young children using an expanded frontal flap and autogenous cartilage framework is a reliable method with great long-term esthetic results. The nasolabial sulcus flap is a relatively simple method, especially for patients with a unilateral nasal alar defect. Supporting structure is needed and appropriate overcorrection is necessary.

Comparison of transferrin isoform analysis by capillary electrophoresis and HPLC for screening congenital disorders of glycosylation.

Transferrin, a major glycoprotein has different isoforms depending on the number of sialic acid residues present on its oligosaccharide chain. Genetic variants of transferrin as well as the primary (CDG) & secondary glycosylation defects lead to an altered transferrin pattern. Isoform analysis methods are based on charge/mass variations. We aimed to compare the performance of commercially available capillary electrophoresis CDT kit for diagnosing congenital disorders of glycosylation with our in-house optimized HPLC method for transferrin isoform analysis. The isoform pattern of 30 healthy controls & 50 CDG-suspected patients was determined by CE using a Carbohydrate-Deficient Transferrin kit. The results were compared with in-house HPLC-based assay for transferrin isoforms. Transferrin isoform pattern for healthy individuals showed a predominant tetrasialo transferrin fraction followed by pentasialo, trisialo, and disialotransferrin. Two of 50 CDG-suspected patients showed the presence of asialylated isoforms. The results were comparable with isoform pattern obtained by HPLC. The commercial controls showed a <20% CV for each isoform. Bland Altman plot showed the difference plot to be within +1.96 with no systemic bias in the test results by HPLC & CE. The CE method is rapid, reproducible and comparable with HPLC and can be used for screening Glycosylation defects.

Cross finger flap cover for fingertip injuries

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;span lang="EN-IN"&gt;Fingertip injuries are the most common form of hand injuries. Restoration of the anatomy is a technical challenge and is one of the basic tenets of the management of fingertip injuries. The cross finger flap is simple and easy to do and is a very useful tool in reconstruction of the pulp of the finger. The project aimed to study the efficacy of cross finger flap in the coverage of fingertip injuries.&lt;/span&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; &lt;span lang="EN-IN"&gt;The study was conducted on a prospective basis in Sri Ramachandra University Hospital in Chennai. The study period was from May 2013 to August 2016. All adults who had fingertip injuries who had undergone cross finger flap cover were included in the study. Patients who had other flaps for reconstruction and who were lost for follow-up were excluded from the study. Preoperative parameters which were recorded were mechanism of injury, size of defect, size of the flap, location of donor site and method of coverage of secondary defect. Outcome measures recorded were flap viability, flap sensibility using two-point discrimination, range of motion of fingers, grip strength and presence of other complications&lt;/span&gt;.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; &lt;span lang="EN-IN"&gt;40 patients were included in the study. Six were lost for follow-up. Mean follow-up was 14 months. All flaps survived. Full range of motion was obtained in 87% of patients. Average grip strength was 94% for dominant hand injuries and 78% for non-dominant hand injuries. Mean quick DASH score was 10.5. Mean two-point discrimination was 15.4 mm. &lt;/span&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; &lt;span lang="EN-IN"&gt;Cross finger flap is very effective for coverage of volar fingertip defects with minimal residual problems.&lt;/span&gt;&lt;/p&gt;

Cooperative effects of zeolite mesoporosity and defect sites on the amount and location of coke formation and its consequence in deactivation

The cooperative effects of secondary mesoporosity and defects on the coke formation and deactivation of zeolite were investigated in n-pentane aromatization using model Ga/ZSM-5 catalysts. Mesoporosity was introduced to a commercial ZSM-5 by alkaline desilication, while the defects sites (i.e., internal silanols) in zeolites were annealed by the treatment with ammoniumhexafluorosilicate. Aromatization after supporting Ga showed that the mesopore generation retarded catalyst deactivation via the suppression of internal coke formation while facilitating the external coke formation due to the enhanced diffusion of coke precursors (e.g., alkylaromatics) out of zeolite micropores. Internal coke can cause a more severe deactivation than external coke because the acid sites are mainly located in the zeolite micropores. However, the mesopore generation alone could not efficiently reduce the total amount of coke deposition (it mainly changed the location of coke). In contrast, the annealing of internal silanols could suppress the overall coke formation. This phenomenon could be explained by the fact that coke precursors are initially generated at the zeolite internal defects, and then deposited as coke at either the internal or the external surfaces of the zeolite depending on the relative kinetics of coke precursor diffusion and its polymerization. Consequently, the catalyst with mesoporosity and annealed internal silanols exhibited the slowest catalyst deactivation due to the suppression of both internal and external coke depositions.

Growth and Psychological Development in Postoperative Patients With Anterior Encephaloceles

PurposeAnterior encephaloceles are rare malformations that are frequently associated with other brain anomalies. This study evaluates the growth and psychological development of children following encephalocele repair. Materials & MethodsGrowth and psychological assessment was done in 24 children with only encephalocele (group I); nine children with encephalocele and hydrocephalus (group II); seven children with encephalocele, hydrocephalus, and secondary malformations (group III); and 40 apparently healthy control subjects. Psychological assessment was done by evaluating intelligence and temperament. ResultsSingle-stage repair was performed in 38 children, and two underwent multistage repair. Major postoperative complications were noted in three individuals. The follow-up period ranged from 12 to 168 months, and during this time the growth velocity declined significantly among group II and group III patients when compared with control subjects. After adjusting the body mass index for age, our data revealed that group III participants had a significantly (P = 0.02) lower body mass index than the control group. Group III also had poor indices for intelligence quotient (P ≤ 0.01) and temperament (P ≤ 0.01). Female patients had lower temperament indices when compared with unaffected females with regard to approach withdrawal (P ≤ 0.01), mood (P = 0.026), and intensity (P = 0.03). Overall, increased disease severity adversely affected the psychological indices. ConclusionIndividuals with anterior encephalocele without associated intracranial defects had excellent postoperative outcomes in terms of growth and psychological developments. Hydrocephalus and agenesis of corpus callosum had the least impact on psychological development. However, the presence of secondary brain defects led to developmental delays. Gender differences in temperament may suggest a need for distinct treatment regimens to assess psychosocial well-being for males and females.

Autogenous Bone Reconstruction of Large Secondary Skull Defects

The authors sought to ascertain the upper limits of secondary skull defect size amenable to autogenous reconstructions and to examine outcomes of a surgical series. Published data for autogenous and alloplastic skull reconstructions were also examined to explore associations that might guide treatment. A retrospective review of autogenously reconstructed secondary skull defects was undertaken. A structured literature review was also performed to assess potential differences in reported outcomes between autogenous bone and synthetic alloplastic skull reconstructions. Weighted risks were calculated for statistical testing. Ninety-six patients underwent autogenous skull reconstruction for an average defect size of 93 cm (range, 4 to 506 cm) at a mean age of 12.9 years. The mean operative time was 3.4 hours, 2 percent required allogeneic blood transfusions, and the average length of stay was less than 3 days. The mean length of follow-up was 28 months. There were no postoperative infections requiring surgery, but one patient underwent secondary grafting for partial bone resorption. An analysis of 34 studies revealed that complications, infections, and reoperations were more commonly reported with alloplastic than with autogenous reconstructions (relative risk, 1.57, 4.8, and 1.48, respectively). Autogenous reconstructions are feasible, with minimal associated morbidity, for patients with skull defect sizes as large as 500 cm. A structured literature review suggests that autogenous bone reconstructions are associated with lower reported infection, complication, and reoperation rates compared with synthetic alloplasts. Based on these findings, surgeons might consider using autogenous reconstructions even for larger skull defects. Therapeutic, IV.

Discussion: Autogenous Bone Reconstruction of Large Secondary Skull Defects.

Discussion: Autogenous Bone Reconstruction of Large Secondary Skull Defects.

Study of surface sulfurization of Cu2ZnSn(S, Se)4 thin film solar cell by sequential H2Se-selenization/H2S-sulfurization

Effect of surface sulfurization on the solar conversion efficiency of Cu-Zn-Sn (CZT) alloy film, which was prepared by DC-magnetron sputtering with a single Cu-Zn-Sn target, followed by a H2S-sulfurization-after-H2Se-selenization (SAS) process to convert the alloy film to a Cu2ZnSn(S, Se)4 (CZTSSe) absorber, was investigated. Compared with a conventional Cu2ZnSnSe4 (CZTSe) solar cell selenized in H2Se, the conversion efficiency of CZTSSe one prepared in the SAS process was increased from 2.1% to 7.5% (increased by 257%). Conductive atomic force microscopy exhibited that the SAS process effectively reduced the leakage current near the grain boundary, while the external quantum efficiency measurement clearly demonstrated that the SAS process increased the energy bandgap by ~0.13eV and significantly reduced the surface defects in the shorter wavelength region. Raman and temperature-dependent photoluminescence spectroscopies elucidated that the SAS process also led to secondary phases (e.g., SnS, ZnS and Cu2SnS3) and structural defects (e.g., ZnSn), which are considered to be a key to achieve a highly efficient CZTSSe solar cell in the future.

Analysis of DNA methyltransferase 3A gene mutations in patients with Philadelphia-negative myeloproliferative neoplasms

Context: Philadelphia (Ph)-negative myeloproliferative neoplasms (MPNs), including essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF) from a group of disorders characterized by dysregulated JAK-STAT functionality, abnormal hematopoiesis, as well as increased production of proliferative cytokines. In addition to JAK2V617F mutation, additional gene alterations that are involved in epigenetic mechanisms, particularly de novo DNA methyltransferase 3A (DNMT3A), have been described in Ph-negative MPN biology. Aims: The aim of this study is to evaluate the H/C/S/P mutations in codon R882 of DNMT3A gene among patients with Ph-negative MPNs. Subjects and Methods: This study was conducted on 64 newly diagnosed patients with PV, ET, and PMF who referred to Shafa Hospital, Ahvaz, Iran. In the beginning, 5 mL whole blood was drawn from each patient, and the DNMT3A R882 codon mutations were investigated following the isolation of peripheral blood mononuclear cells by DNA amplification protocol using polymerase chain reaction and DNA sequencing techniques. Results: The R882H G > A mutation, which results in an amino acid substitution at position 882 of DNMT3A gene from arginine (R) to histidine (H), was observed in two patients (3.1%) with JAK2V617F positive PV and JAK2V617F negative PMFs. Conclusions: Based on the results, DNMT3A-R882 mutations occur at a low frequency in patients with Ph-negative MPNs. To the best of our knowledge, this is the first study to specifically estimate the prevalence of DNMT3A mutations among Ph-negative MPN patients living in the Middle East. It is recommended to investigate these mutants as a secondary defect along with common major complications in such patients.

The use of vacuum-assisted wound closure in musculoskeletal injuries

Background: This study was performed to evaluate the results of vaccum assisted wound therapy in patients with open musculoskeletal injuries or infected or exposed implant in situ (excluding arthroplasty) or traumatic wounds after debridement, infections after debridement at a tertiary care hospital, from 2013 to 2015. Materials and Methods: 23 Patients were prospectively treated using NPWT{Negative Pressure Wound Therapy}.Mean Age of patient was 39±18yrs yrs (range,18 to 76yrs).All patients had wounds with underlying tendon or bone exposure due to RTA OR CRUSH INJURY or ACUTE TRAUMA had to be debrided before applying NPWT.T The optimal level of negative pressure appears to be around 125mmhg and is more effective as evidenced if applied in a cyclical fashion at least three times a day for 15 mins either continous or intermittent. In our study we use a sterile polyurethane sponge which is cut to suit th wound surface and placed to contact with entire wound surface. A romovac suction drain tube runs from the sponge to an another tube, which is connected to suction apparatus sponge. An adherent plastic sheet (cling drape or op sites) is used to adhere sponge to surrounding skin to form a closed system of wound. Results: Exposed tendons, bones, raw area wounds and exposed implants were covered by granulation tissue successfully and was managed by either a skin graft or other definitive procedures. No major complication occurred that was directly attributable to treatment. Conclusion: NPWT was found to facilitate rapid formation of granulation tissue on wounds with Exposed tendons, bones, raw area wounds and exposed implants hence shorten healing time and minimize secondary soft tissue defect coverage procedures. The technique we used is indigenous and modified as per our situation. This technique can be used in treatment of many of soft tissue injuries including degloving injuries, wounds and various soft tissue injuries prior to surgical closure, grafting or reconstructive surgeries. VAC is a cost effective and economical mode of treatment with suggestive evidence when compared with other conventional therapies with the added advantage of reduced hospital stay, no need for plastic surgery and better cosmetic acceptance.

Radiation Defects Nano-Scale Inhomogeneous Distribution Influence on Apparent Hall Mobility in Silicon

Temperature dependencies of charge carrier Hall concentration and Hall mobility are systematically studied within the range 77 to 300 K for both n- and p-type silicon crystalline samples irradiated with high energy protons or electrons and annealed at temperatures up to 380°C and 600°C, respectively. In some cases, measured at a fixed temperature hall mobilities are found to be significantly lower or significantly higher than those in non-irradiated material. These results are interpreted on the basis of assumption that secondary radiation defects in silicon form clusters with conductivity significantly different from that of the surrounding matrix. A phenomenological theory, recently introduced by authors, explains apparent Hall mobility minima revealed on the temperature-dependence curves. Clusters themselves should possess high conductivity (be “metallic”), but in certain conditions they effectively act as (“dielectric”) clusters with low conductivity, because they can be screened by the shells consisting of defects capturing charge carriers.

Strategic review of interface carrier recombination in earth abundant Cu–Zn–Sn–S–Se solar cells: current challenges and future prospects

The article presents a strategic review of secondary phases, defects and defect-complexes in kesterite CZTSSe solar cells responsible for performance gap compared to CIGS solar cells.

Pdgfrα functions in endothelial-derived cells to regulate neural crest cells and the development of the great arteries.

ABSTRACTOriginating as a single vessel emerging from the embryonic heart, the truncus arteriosus must septate and remodel into the aorta and pulmonary artery to support postnatal life. Defective remodeling or septation leads to abnormalities collectively known as conotruncal defects, which are associated with significant mortality and morbidity. Multiple populations of cells must interact to coordinate outflow tract remodeling, and the cardiac neural crest has emerged as particularly important during this process. Abnormalities in the cardiac neural crest have been implicated in the pathogenesis of multiple conotruncal defects, including persistent truncus arteriosus, double outlet right ventricle and tetralogy of Fallot. However, the role of the neural crest in the pathogenesis of another conotruncal abnormality, transposition of the great arteries, is less well understood. In this report, we demonstrate an unexpected role of Pdgfra in endothelial cells and their derivatives during outflow tract development. Loss of Pdgfra in endothelium and endothelial-derived cells results in double outlet right ventricle and transposition of the great arteries. Our data suggest that loss of Pdgfra in endothelial-derived mesenchyme in the outflow tract endocardial cushions leads to a secondary defect in neural crest migration during development.

Differentiation in body and testes weight as well as sperm quality of male roe deer from the Lublin Upland in the reproductive season

The aim of the study was to evaluate the morphology structure of sperm cells and quality of individual male deer obtained in the Lublin Upland during the 2015/2016 hunting season. The comparison included two sepa-rate periods of the hunting season resulting from varying physiological processes related to the estrus period of this species. In the first study with regards to the estrus period, males were characterized by an average of 1.6 grams less testicle weight, while body weight was 1.9 kg higher. Increasing the mass of the testicles in the repro-ductive season proceeded simultaneously with the increase in the share of properly developed sperm cells to the level of 70.6%. During the pre-estrus period, the percentage of spermatozoids with main and secondary defects accounted for almost 60%. Between the compared periods, an approximately 6.8% body weight loss occurred. The highest percentage of properly developed spermatozoids was present in deer aged 4 and 5 years. Regarding the greatest body weight loss in these two age groups, it is indicated that this age is the climax in terms of poten-tial accession to the breeding and effective mating of females and the possibility of fertilization. Analysis of the relationship between body weight and testicle weight showed low and statistically insignificant correlation coef-ficients (rxy=0.331-0.393) between those features.

Surgical management of nonmelanomatous malignant cutaneous lesions of the face

Objective The aim of the present study was to evaluate the esthetic and functional outcomes after surgical excision of nonmelanomatous malignant lesions of the face, followed by a suitable tool of reconstruction. Background Neoplasm of the skin is found most often on the face. Cosmetically, the face is the most important anatomic area for most patients. Because of this, malignant tumors of the facial skin pose a great challenge in treatment, prohibiting compromises between oncologically responsible surgery and functional and cosmetic outcomes. Patients and methods This prospective study included 25 patients, all of them suffering from nonmelanomatous skin cancer and managed at Menoufia University Hospitals and Damanhur Oncology Center by different modalities of reconstruction during the period from April 2012 to December 2014. The appropriate technique was selected for every patient, taking in consideration the location and size of the lesion. Results Eighteen cases had reconstructive procedures with local flaps only, five cases had more combined reconstructive tools in the form of local flaps (rotational flaps) plus split-thickness graft to close the secondary defect, and two cases required reconstruction with a full-thickness graft. Conclusion Our experience showed that the postoperative defects gave the best cosmetic results when reconstructed by using a nearby tissue, fulfilling all patient and surgeon expectations with a minimum rate of morbidity. Clear guidelines and a multidisciplinary approach for the treatment of skin cancer are important to ensure that patients receive optimal care with satisfactory end results for tumor control as well as reconstructive esthetic outcome.

CARDIAC ARRHYTHMIAS AFTER TRANSCATHETER CORRECTION IN PATIENTS WITH SECONDARY ATRIAL SEPTAL DEFECT

SUMMARY In order to study the dynamics of cardiac arrhythmias in patients with secondary atrial septal defect (ASD) examined 50 patients in the age of from 3 to 68 (mean age 24,2±2,1 years) with secondary defect investigated arrhythmias changes in the nearest (till 3 months) and remote (till 1 year) the periods after transcatheter closings of arial septal defect using the amplatzer septal occluder were examined. Already in 3 day after transcatheter correction marked decrease frequent (67,8 ±29,4 till 62,5±25 in hour) group supraventricular extrasystoles, disappear episodes paroxysmal atrial fibrillation and paroxysmal supraventricular taxycardias. By 6 months the disappearance of the group and reduction of frequent supraventricular extrasystoles. In relation to ventricular arrhythmias on day 3 observations marked deerease in the number of VEs (254,1±171,2 till 32,6±3,6 in hour), the complete disappearance of VEs high grade to 12 months. Thus, after transcatheter closure of ASD in the coming secondary (3 hours) and long-term (6-12 months). Deadlines are not observed occurrence of serious cardiac arrhythmias, and available undergoing a significant improvement towards a significant reduction in the number of premature beats until their complete disappearance. This fact confirms the effectiveness and safety of this method of treatment of secondary ASD. Key words: secondary interatrial septum defect, electrocardiographia, Amplatzer occluder, supraventricular and ventricular extrasystols.