DPPH Radical Scavenging Activity Research Articles

Caviar extract inhibitsskin photoaging by activating skin stem cells through NF-κB/MMPs/COL17A1 axis.

Ultraviolet radiations (UVR) produce harmful entities and reactive oxygen species (ROS) in skin cells, leading to skin photoaging. Caviar extract(CE) showed outstanding effects in delaying skin aging, but the underlying mechanism remains largely unknown. In this study, we prepared CE with acid protease and examined the anti-skin photoaging effects. The results showed that CE performed no cytotoxicity to HaCaT cells. For antioxidant properties, the EC50 values of DPPH and ABTS radical scavenging activity for CE were 1.27 and 5.20 mg/mL, respectively. It significantly reduced NF-κB, MMP-3 and MMP-9 protein expression levels, and increased IκB and TIMP-1 expression level in UVA-irradiated HaCaT cells. In the skin aging mice model, CE reduced the degree of UV-induced skin photoaging. Histological study confirmed that CE can ameliorate the adverse effects of UV exposure on the skin. Moreover, we found that CE could enhance the activities of Superoxide dismutase (SOD), and increased the contents of hydroxyproline (HYP) in photoaged mice skin. And CE elevated the protein expression level of COL17A1, KRT10, and KRT14 in mice skin. Taken together, our results bright systemic and new insights of CE into preventing UV-induced skin photoaging.

Impact of Lactobacillus rhamnosus GG fermentation on shelf life of Volvariella volvacea paste

AbstractPaste formulations of Volvariella volvacea (VV) were developed for its shelf-life enhancement. Egg stage fruiting bodies of mushrooms were used for preparation of salted, spiced, and their fermented pastes. Fermented paste formulations were developed using inoculation with Lactobacillus rhamnosus GG at 0.5% (w/w) and incubation for 2 days at 35 ± 2 °C. The unfermented VV paste was preserved with sodium benzoate of 0.065%, while fermented formulations were kept devoid of any chemical preservative. All formulations were packed in glass jars and retort bags, and thereafter treated in boiling water bath for one hour. The spiced, fermented VV paste scored highest in sensory evaluation and maximum crude protein and moisture contents, while lowest in contents of ash, fat, crude fibre, and carbohydrates. The fermented (salted and spiced) VV pastes showed variation in pH at 4 °C. Shelf life of 56 days was observed for fermented VV paste stored in glass jars under refrigerated conditions. The antioxidant analysis revealed that DPPH radical scavenging activity of fermented paste formulations increased significantly up to 21 days followed by gradual decrease until the 56th day, while total phenolic content of all paste formulations decreased with storage time. Hence, fermented spiced paste showed enhanced nutritive and antioxidative potential and shelf life up to 56 days.

Antioxidant, anti-prostate cancer potential, and phytochemical composition of the ethyl acetate stem bark extract of Boascia coriacea (Pax.).

The antioxidant and anticancer potential of natural compounds, particularly from medicinal plants, is increasingly being explored as alternatives to synthetic antioxidants and chemotherapeutics. Boascia coriacea (Pax) has been traditionally used for treating various ailments, including oxidative stress-related diseases and prostate cancer. However, there is a paucity of empirical evidence to validate the ethnomedicinal claims, hence this study. The antioxidant capacity of the extract was assessed using 1,1-Diphenyl-2-picryl Hydrazyl radical (DPPH) radical scavenging and hydrogen peroxide scavenging assays, alongside total antioxidant capacity. In vitro cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Vero CCL-81 normal cells and DU-145 prostate cancer cells. Gene expression levels of ar, bcl-2, caspase 3, cdk1, and p53 were quantified using qPCR to elucidate the mechanisms of action. Phytochemical analysis was conducted using gas chromatography-mass spectrometry. The studied plant extract exhibited significant DPPH radical scavenging activity, with an EC50 of 0.008 μg/ml, 10-fold lower than that of L-ascorbic acid (0.08 μg/ml), indicating potent antioxidant capacity. Similarly, the extract demonstrated substantial hydrogen peroxide scavenging activity, albeit with lower efficacy (EC50 of 1039.10 μg/ml) compared to L-ascorbic acid, and a total antioxidant capacity of 454.39±25.26 μg AAE/mg dw. In vitro cytotoxicity assay revealed a CC50 of 68.61 μg/ml against Vero CCL-81 cells and an IC50 of 32.16 μg/ml against DU-145 cells, with a superior selectivity index of 2.13, compared to doxorubicin's 1.46. The extract significantly downregulated the expression of ar, bcl-2, normalised caspase 3, cdk1 genes while upregulating p53 in DU-145 cells, suggesting its role in inducing apoptosis and inhibiting cancer cell proliferation. Phytochemical analysis identified 19 compounds, including lup-20(29)-en-3-one (7.99%) and lupeol (59.49%), which are associated with anticancer activity. The ethyl acetate stem bark extract of B. coriacea demonstrates significant antioxidant and anticancer activities, potentially through modulation of apoptosis and cell cycle pathways. The presence of bioactive compounds supports its potential as a therapeutic agent, warranting further investigation for developing novel treatments for prostate cancer and oxidative stress-related conditions.

TOTAL POLYPHENOL CONTENT AND ANTIOXIDANT ACTIVITY OF RUTABAGA (BRASSICA NAPUS L. VAR. NAPOBRASSICA) ROOTS

This study aims to determine the total content of polyphenols and the antioxidant activity by DPPH spectrophotometrically in rutabaga (Brassica napus L. var. napobrassica) samples, cultivated in the Trenčín region, in the Púchov district, at the border of the White Carpathian and Javorníky mountains, Slovakia. For analysis were used three types of rutabaga, named after the collection sites (Mestečko, Lúky 1, Lúky 2). The total polyphenol content in rutabaga was ranging from 2.41 (Mestečko) to 2.82 mg GAE.g-1 dry weight (Lúky 2). It was established that that the studied rutabaga samples do not show a significant difference in the total content of polyphenols between the collection locations. In the antioxidant activity of rutabaga, differences were confirmed in AAs. A statistical difference was established between locations Lúky 2 and Mestečko. DPPH radical scavenging activity values were from 5,19 (Mestečko) to 7,49 µmol TE.g-1 DW (Lúky 2). Spearman's test showed a positive relationship between both methods used. These results confirm the promising potential of rutabaga use in functional food preparation.

Antioxidant and antimicrobial activities of different extracts of Tragopogon dubius and Tragopogon porrifolium L. subsp. longirostris: Determination of their phytochemical contents by UHPLC-Orbitrap®-HRMS analysis

In this study, dichloromethane (DCM), n-hexane, ethanol, methanol, and natural deep eutectic solvents (NADES) were used to extract Tragopogon porrifolium L. subsp. longirostris (Sch. Bip.) Greuter and Tragopogon dubius L. Antioxidant properties (Cu2+ reduction capacity, DPPH, ABTS and Fe3+ reduction) and antimicrobial activities of these extracts were determined. Also, metabolite profiles were determined using UHPLC-Orbitrap®-HRMS. NADES extracts of both species (MIC: 0.18-0.71 mg/ml) showed significantly better antimicrobial activity against Klebsiella pneumoniae, Bacillus subtilis, and Staphylococcus aureus compared to other solvents (MIC: 25 mg/ml). The study showed that T. dubius methanol and NADES extracts had better inhibitory effects on DPPH (EC50:45.17 μg/mL, EC50:108.73 μg/mL) and ABTS (EC50:59.54 μg/mL, EC50:95.98 μg/mL) radical scavenging activities than other extracts. Similarly, T. porrifolium methanol and NADES extracts had better inhibitory effects on DPPH (EC50:48.33 μg/mL, EC50:38.72 μg/mL) and ABTS (EC50:77 μg/mL, EC50:58.93 μg/mL) radical scavenging activities than other extracts. Furthermore, Fe3+ and Cu2+ reducing capacities were highest in methanol and NADES extracts of both plants.UHPLC-Orbitrap®-HRMS was used to profile the chemical composition, validating 71 phytochemicals, with the R2 value ranging from 0.9903 to 0.9993. The method's LOD values ranged from 0.45 to 2.94, and LOQ values ranged from 1.50 to 9.78. Recovery percentages at 10, 60, and 100 ng/mL concentrations ranged from 71.73% to 116.91%, 85.36% to 115.38%, and 97.08% to 109.65%, respectively. The analysis identified 29 phytochemicals, with major compounds including chlorogenic acid, quinic acid, apigenin 7-glucuronide, vitexin, schaftoside, orientin+isoorientin, luteoloside, and isoquercitrin. The significant biological impacts of these plants as a compelling reservoir of phytochemicals with therapeutic properties.

Design, spectral technique, anti-diabetic, anti-inflammatory, antioxidant, and molecular docking investigations of acridine derivatives

A novel acridine derivative was synthesised by conventional method. Every synthesised molecule was analysed by IR, 1H, 13C NMR and mass spectral techniques. Biological studies like anti-inflammatory and anti-oxidant activities with DPPH radical scavenging activity, anti-diabetic properties, and molecular docking were examined. The anti-diabetic properties of acridine derivatives indicate that compounds 2 and 7 exhibit greater activity compared to the typical acarbose one, with IC50 values of 26 and 25, respectively. According to in-vitro investigations on anti-inflammatory effect, compounds 4 and 6 have larger inhibition percentages than the other compounds. The oxidoreductase tuberculosis protein is tightly docked with the synthesised moieties. Compound 7 exhibits the highest inhibition constant of 608.73 μM. The remarkable anti-inflammatory and antioxidant properties, together with its synthesis and characterization, highlight its potential as a viable candidate for more biomedical research and therapeutic development

Comparative Studies of Chemical Composition and Biological Activity of Juniperus Communis L. Essential Oil From Different Localities in the Republic of Serbia

Abstract This work aims to determine the chemical composition of Juniperus communis (J. communis) essential oil from different localities in the Republic of Serbia and examine its antimicrobial and antioxidative effects together with chemometric analysis and principal component analysis. Essential oils were extracted via hydro distillation from the fruits of J. communis gathered from four different habitats in Serbia. Extraction yields ranged from 1.56 % in the sample from Bavanište to 1.98% in the sample from Mačkat. A total of 23 compounds in the four essential oil samples were identified by Gas Chromatography-Mass Spectrometer (GC-MS). The dominant compounds were α-pinene (32.68–51.10 %), β-phellandrene (6.43–24.77 %), and β-pinene (9.84–14.09 %). Compared with the other ecological factors, precipitation showed a strongly positive correlation (0.871) with essential oil yields. The four samples could be classified into two clusters based on the variance in their components. All the essential oils samples showed bioactivities. Among them, the essential oil from the Mačkat sample showed the best ABTS radical scavenging activity (IC50=237.74 μg/ml), DPPH radical scavenging activity (IC50=308.83 μg/ml) and antimicrobial activities. Consequently, the essential oil extracted from Mačkat has the potential for commercial viability in the food, cosmetic, or medical fields.

Glacium flavum‐Derived Phytochemical Compounds and Their Molecular Interactions with SIRT1

AbstractThis study investigates the antioxidant activity, phenolic content, and phytochemical components of Glacium flavum root extract. The DPPH radical scavenging activity was found to be 87.01 µg/mL ± 4.98, iron chelating activity 7.49 mg/mL ± 0.36, total phenolic content 594.39 mg GAE/g extract ± 17.90, and total flavonoid content 44.30 mg QE/g extract ± 4.76. These results indicate that G. flavum root extract possesses significant antioxidant properties and rich phytochemical components. Gas chromatography–mass spectrometry (GC–MS) analysis identified 11 biologically active components. Fatty acid composition analysis revealed lauric acid (1.26%), myristic acid (1.20%), palmitic acid (14.58%), stearic acid (3.40%), oleic acid (4.41%), linoleic acid (20.00%), and alpha‐linolenic acid (41.43%). Molecular docking studies demonstrated significant binding interactions of alpha‐linolenic acid and ethyl oxirane‐2‐carboxylate with the SIRT1 catalytic domain (4I5I), with binding energies of −4.5 kcal/mol and −4.2 kcal/mol, respectively. Additionally, (2S)‐2‐hydroxybutanedioic acid and butane‐2,3‐diol also exhibited notable interactions. Toxicity profiles showed that alpha‐linolenic acid and butane‐2,3‐diol have lower toxicity, while 2‐ethyl oxirane‐2‐carboxylate and (2S)‐2‐hydroxybutanedioic acid displayed moderate toxicity. Drug‐likeness evaluations indicate that the compounds meet various medicinal chemistry criteria, each facing development challenges. Overall, this study provides a comprehensive overview of the antioxidant potential, biologically active components, and toxicological characteristics of G. flavum root extract.

Unifying Theory and Experiments: Multi-Target Pharmacology of Dajihan Pill Against Hyperlipidemia

Background: The increasing incidence of hyperlipidemia (HLP) is attributed to the imbalance in redox homeostasis, aberrant lipid metabolism, and the excessive intake of empty calories. Dajihan Pill (DJHP) is a Traditional Chinese Medicine (TCM) formula composed of Zingiberis Rhizoma (ZR), Piperis Longi Fructus (PLF), Alpiniae Officinarum Rhizome (AOR), and Cinnamomi Cortex (CC) in a ratio of 3:2:3:2. It exhibits a significant preventive effect on HLP. Certainly, the active components and the precise mechanism of action are not fully understood. Therefore, this study aims to elucidate the preventive and ameliorative mechanisms of DJHP against HLP by integrating network pharmacology, molecular docking, and experimental validation. Methods: Based on the pharmacological method, active ingredients in DJHP and targets were extracted from Traditional Chinese Medicine System Pharmacology (TCMSP) and UniProt. Then core compounds and targets were obtained by constructing “compounds-targets-disease” and proteinprotein interaction (PPI) network. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to elucidate further the associated action mechanism. The molecular binding mechanisms between the core ingredients and targets were elucidated through molecular docking. Additionally, the antioxidant capacities of DJHP extracts were investigated by assessing their DPPH, hydroxyl, and ABTS radical scavenging activities. Results: A total of 45 active compounds and 258 targets were identified in DJHP. Network analysis indicated that quercetin, beta-sitosterol, kaempferol, and oleic acid might serve as core bioactive compounds. Seven core targets, including AKT1, INS, and TNF, were identified as potential preventive targets. GO analysis suggested the improvement of HLP by DJHP may be related to the lipid metabolic process, high-density lipoprotein particle, triglyceride binding, and inflammatory response. The KEGG analysis indicated TNF, HIF-1, and AMPK signaling pathways were involved. The observations of active compounds binding with core targets indicated an excellent combination. Additionally, antioxidant results showed that DJHP exhibited significant DPPH, hydroxyl, and ABTS radical scavenging activities. Conclusion: Theoretical and experimental investigations indicate that DJHP can effectively modulate various signaling pathways and enhance the redox system, thus mitigating HLP. Our work provided a basis for the pharmacological study of DJHP in preventing HLP and further research.

Formulation of Solid Lipid Nanoparticles Loaded with Rosiglitazone and Probiotic: Optimization and In-vitro Characterization.

In the present study, solid lipid nanoparticles loaded with Rosiglitazone and probiotics were prepared via solvent emulsification diffusion method which is patented. As a lipid and surfactant, Gleceryl monostearate and Pluronic -68 were used in the formulation process. During characterization, it was determined that ingredient quantity variations significantly impacted Rosiglitazone loading capacity, particle size, polydispersity index, etc. In an optimized formulation of RSG-PB loaded SLNs, spherical particles with a mean particle size of 147.66 ± 1.52 nm, PDI of 0.42 ± 0.02, and loading capacity of 45.36 ± 0.20 were identified. Moreover, the developed SLNs had the potential to discharge the drug for up to 24 hours, as predicted by Higuchi's pharmacokinetic model. The SLNs were stable at 25°C/60%RH for up to 60 days. There was little to no change in particle size, PDI, or loading capacity. In addition, the number of probiotic bacteria was determined using the standard plate count procedure. Further, the antioxidant effect of the prepared formulation is evaluated using the DPPH assay method. This study concludes that the method used to fabricate RSG-probiotic-loaded SLNs is straightforward and yields favorable results regarding various parameters, including sustained release property, particle size, PDI, and percent drug loading stability. Furthermore, DPPH radical scavenging activity shows the high antioxidant potential of RSG-PB SLNs when compared to RSG and probiotics alone.

Siamese neem tree as a natural preservative: Chemical profile, antioxidant properties, and antibacterial efficacy against foodborne pathogens and spoilage bacteria

The objective of the present study was to investigate the effect of various ethanol in water solvent systems (0, 25, 50, 75 and 100% v/v) on the extraction crude yield, total phenolic content, total flavonoid content, and chemical profile of Azadirachta indica (Siamese neem tree) leaf extracts. In addition, the antioxidant and antimicrobial properties were investigated using in vitro assays. Crude yield was highest for the water extract (5.78 g/100 g DW) and decreased with increased ethanol. Total phenolic and flavonoid contents were highest in the 75% ethanol extract (26.95 mg GAE/g crude and 23.73 mg QE/g crude, respectively). GC-MS analysis of the 75% ethanol extract revealed 33 bioactive compounds, accounting for 96.33% of the total area. Significant components included benzene, 1,4-bis(phenylmethyl)- (15.05%), and naphthalene, 1,6-dimethyl- (10.98%); also present were sterols, fatty acids, hydrocarbons, aromatic, and heterocyclic compounds, indicating a complex chemical profile. In antioxidant assays, the 75% ethanol extract revealed the highest DPPH radical scavenging activity (IC50: 143.03 mg/L), reducing power (IC50: 378.69 mg/L), and lipid peroxidation (IC50: 526.25 mg/L). However, the absolute ethanol extract exhibited both the highest metal chelating activity (IC50: 382.60 mg/L) and the strongest antimicrobial activity, inhibiting all 11 tested foodborne pathogenic and spoilage bacteria. The lowest minimum inhibitory concentration (MIC) of 25 mg/mL was observed for Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas fluorescens, Latilactobacillus sakei, Streptococcus sp., and Lactococcus cremoris, with inhibition zone diameters of 27.21, 28.79, 28.19, 17.60, 15.09 and 11.20 mm, respectively. Similarly, the lowest minimum bactericidal concentration (MBC) of 25 mg/mL was obtained for S. aureus and L. sakei, indicating potent antibacterial effects against these species. Future studies should include direct comparative analyses of neem extracts and synthetic preservatives to evaluate further their relative efficacy and applicability in real-world food systems.

In vitro Study on Antioxidant and Antiglycation Activities, and Molecular Docking of Moroccan Medicinal Plants for Diabetes

Background: Moroccan medicinal plants with historical usage to treat diabetes have been analyzed to explore their potential anti-glycation and antioxidant activities through in vitro experiments. These investigations have been complemented by molecular docking. Methods: The present study has examined the in vitro antioxidant and antiglycation properties of six aqueous extracts from six medicinal plants (Ammodaucus leucotrichus, Thymelaea hirsuta, Arbutus unedo, Urtica dioica, Ptychotis verticillata, and Caralluma europaea), and two seeds oils from Argania spinosa and Opuntia dillenii. The antioxidant activity was determined by using the DPPH radical scavenging activity test and β-carotene/linoleic acid bleaching assay. In addition, the antiglycation activity was detected by using the hemoglobin protein model. Results: All studied plant extracts have shown potent antioxidant and antiglycation activity. Furthermore, to confirm the in silico antiglycation activity of the selected medicinal plants, molecular docking techniques were used assuming that binding energy decreases as compound affinity increases; the best molecules responsible for the remarkable antiglycation activity have also been highlighted. Conclusion: Antidiabetic herbal medicines are responsible for the inhibition of oxidative activity and glycation. The molecular docking analysis showed catechin, kaempferol-O-acetylhexoside, luteolin, luteolin-7-O-glucoside, quercetin, and zeaxanthin, to have a high affinity to AGEs receptor and a potent inhibitory activity.

Olive mill wastewater: From by-product to smart antioxidant material

Olive mill wastewater (OMWW) is a byproduct of olive oil extraction that represents a critical environmental concern due to its potential adverse effects on ecosystems. Given these premises, spray-dried microparticles were designed and developed using maltodextrins as carriers to encapsulate OMWW bioactive compounds. The microparticles were manufactured using an easily scalable and sustainable spray-drying process. The resulting microparticles were smooth, spherical, and exhibited a mean particle size of about 18 μm. The systems demonstrated notable antioxidant properties with a DPPH radical scavenging activity higher than 60 %, due to the polyphenolic compounds of OMWW (about 24 gallic acid equivalents per g of sample). In addition, the microparticles supported fibroblast and macrophage viability at concentrations up to 1 mg/mL. They also determined a 4-fold inflammation reduction in macrophages, improved collagen expression in fibroblasts, and modulated oxidative stress on aged fibroblasts. In conclusion, these microparticles could be considered as promising medical devices in wound healing, while offering a sustainable solution for valorizing OMWW.

Antioxidant and Antiaging Activity of Houttuynia cordata Thunb. Ethyl Acetate Fraction in Caenorhabditis elegans

Background/Objectives: In aerobic organisms, such as humans, oxygen radicals are inevitably produced. To counteract oxidation, the body generates antioxidant substances that suppress free radicals. However, levels of reactive oxygen species (ROS) increase due to aging and lifestyle factors, leading to exposure to various diseases. While synthetic antioxidants offer advantages like high stability, low cost, and availability, their safety remains controversial. This study aimed to investigate the antioxidant and antiaging activities of Houttuynia cordata (HC), which is rich in flavonoids and has excellent antioxidant properties, using Caenorhabditis elegans as a model. Methods: Extraction and fractionation of HC were performed to evaluate antioxidant activities (DPPH, ABTS, superoxide radical scavenging activity) and antiaging effects (lifespan). The ethyl acetate fraction (EAF) with the highest activity was selected for further investigation. Results: The EAF of HC exhibited high levels of polyphenols and flavonoids, presenting the highest DPPH, ABTS, and superoxide radical scavenging activities. This fraction increased the activity of antioxidant enzymes in nematodes in a concentration-dependent manner and provided resistance to oxidative stress, reducing ROS accumulation. Additionally, the fraction enhanced the lifespan of nematodes, improved resistance to heat stress, increased survival rates, and decreased the accumulation of aging pigments (lipofuscin). The expression of daf-2, daf-16, and sir-2.1, proteins directly involved in nematode aging, was confirmed. Liquid chromatography/tandem mass spectrometry identified quercitrin in the HC extract, which may contribute to its antioxidant and antiaging effects. Conclusions: The EAF of HC demonstrates significant potential for influencing antioxidant and antiaging, as evidenced by functional investigations using C. elegans.

Investigating effects of air-cold plasma jet on enzymatic activity and nutritional quality attributes of Mangosteen (Garcinia mangostana L.) juice

This study aimed to investigate the effects of air-cold plasma jet processing on the quality of mangosteen juice using an experimental design that varied the duty cycle of the power source (30%–70%), air flow rate (1–5 L/min), and treatment time (2–10 min). Changes in pH, color, ascorbic acid (AA), anthocyanin, flavonoid, phenolic content, enzyme activities, and antioxidant activity were evaluated. The cold plasma jet treatment substantially inactivated polyphenol oxidase, reducing its activity by up to 60% across all tested conditions, while peroxidase activity exhibited varying responses. The optimal treatment conditions (60% duty cycle, 4 L/min air flow rate, and 4 min treatment time) maximized bioactive compound levels, resulting in an 87.75% increase in AA content, along with substantial increases in total anthocyanins, phenolic content, and flavonoids. Cold plasma jet improved antioxidant activity, particularly in the DPPH radical scavenging activity. No notable change in color was observed. This study highlights the effectiveness of cold plasma technology for processing mangosteen juice while preserving its essential nutritional and physical qualities. The method offers a promising nonthermal alternative for maintaining the quality of high-value processing-sensitive mangosteen juice.

Analyzing Quinoa Seeds: LC-MS/MS and GC-MS/MS Approaches for Identifying a Nutrient-Rich Functional Food in Alzheimer's and Diabetes Care.

Quinoa is a versatile, nutrient-dense, gluten-free pseudocereal, increasingly recognized for its potential health benefits, particularly in managing diabetes mellitus and Alzheimer's disease-two conditions with growing links. Recent research on 14 quinoa genotypes explored fatty oil composition and bioactive potential of quinoa extracts. Linoleic acid was predominant fatty acid (46.60%-56.33%), while alanine was the most concentrated amino acid across all active extracts (192.4887-1578.0355 nmol/mL). French Vanilla hexane extract exhibited the strongest α-glucosidase inhibition (75.71%), with Santa Ana hexane extract showing the highest α-amylase inhibition (28.58%). Additionally, Titicaca hexane extract displayed notable acetylcholinesterase inhibition (22.22%), and Moqu Arochilla methanol extract had the most potent butyrylcholinesterase inhibition (47.20%). Antioxidant assays revealed that Salcedo and French Vanilla extracts had exceptional DPPH radical scavenging activity, with positive correlations found between total phenolic content and antioxidant activity (DPPH, β-carotene, FRAP). These findings emphasize quinoa's potential as a functional food, offering significant benefits for disease management.

Antioxidant, anticoagulant and antithrombotic properties of ethanolic extract of Centaurea diluta subsp. algeriensis (Coss. & Durieu) Maire: in vitro study and novel therapeutic approach

The aim of this study is to estimate the antioxidant, anticoagulant and antithrombotic activities of the ethanolic extract of Centaurea diluta subsp. Algeriensis (Coss. & Durieu) Maire, an endemic Algerian plant belonging to Asteraceae family. The antioxidant activity was determined by DPPH radical scavenging activity and metal chelating test. The plasma of healthy volunteers was used to evaluate the clotting time, prothrombin time, and thrombolysis activity. C. diluta ethanolic extract (CEE) showed a good ability to scavenge DPPH radical with an IC50 value of 0.115 ±0.005 mg/ml. In addition, this extract exhibited a high iron chalating capacity with IC50 of 0.36 ± 0.07 mg / ml. Also, the results showed that CEE exhibited anticoagulant activity by prolonging the clotting time at the concentration of 50 mg/ml. For prothrombin time, the concentration of 50 mg/ml has a strong effect with value of 13.14±1.91 sec. In the thrombolysis test, the CEE extract showed a moderate thrombolytic activity with the best effect at a concentration of 50 mg/ml (20.86±0.31%).These findings could pave the way for the development of natural antioxidant and anticoagulant agents that can be used in the prevention and treatment of thrombotic disorders.

Optimization of Extraction Conditions of Phenolic Compounds and Antioxidant Activity from Myrtle (Myrtus communis L.) Fruit.

The extraction of polyphenols from myrtle (Myrtus communis L.) fruits using a conventional solid-liquid extraction was optimized using a single factor experiment approach. The influence of several parameters such as type of solvent (50% acetone, 50% methanol, 50% ethanol and water), solvent concentration (30-100%; v/v), solvent acidity (0-0,1 N), temperature (20-40°C) and time (30-360 min) on the yield extraction of total phenolic content (TPC), total flavonoid content (TFC), total proanthocyanidin content (TPAC), and on the antioxidant activity : DPPH-radical scavenging activity (DPPH-RSA), ABTS-radical scavenging activity (ABTS-RSA) and ferric reducing power (FRP) was studied. The results showed that 50% acetone, 40°C and 180 min were the best conditions for extracting TPC (87 mg GAE/g DW), TFC (12 mg QE/g DW), and TPAC (76 mg CE/g DW). Myrtle berries extracted with these conditions also exhibited the strongest antioxidant activity: DPPH-RSA (7166 mg TE/g DW), ABTS-RSA (164 mg TE/g DW), and FRP (69 mg AAE/g DW). Pearson correlation coefficients showed a good positive correlations between TPC, TFC and TPAC and antioxidant activities (DPPH-RSA, ABTS-RSA and FRP), mainly under the effect of solvent type (0.93 < r < 0.99) and solvent concentration (0.91 < r < 0.99).

Intelligent active packaging of sodium alginate and pectin mixed with Aronia melanocarpa anthocyanins and tea polyphenols for shrimp freshness monitoring and preservation

In this study, an intelligent active packaging film was developed using sodium alginate (SA) and pectin (PC) as the film matrix, with Aronia melanocarpa anthocyanins (AMA) as a pH-sensitive indicator and tea polyphenol (TP) added to stabilize the anthocyanins. The results demonstrated that AMA and TP formed hydrogen bonds with polysaccharides, which reduced the surface roughness of the film and enhanced the compatibility of the component. The interaction between TP and AMA improved the stability of AMA, leading to an increase in anthocyanin retention rate from (29.56 ± 1.22)% to (40.67 ± 1.83)% after 4 days of UV irradiation. The addition of TP significantly enhanced the tensile strength (from 3.13 MPa to 4.26 MPa), UV-blocking properties, and antioxidant activity (with DPPH and ABTS radical scavenging activities being 4.8 and 9.6 times higher than those of the SA/PC film), as well as the antibacterial properties of the film. Additionally, the film exhibited a distinct color response to pH changes. Finally, the films were successfully applied to preserve shrimp and provide real-time visual monitoring of freshness. The results indicated that the SA/PC/AMA-2/TP film extend the shelf life of shrimp by approximately 12 h compared with the control group, making it a promising new food packaging material with potential applications.

Upgrading the functional properties of apricot kernel proteins through fibrillization

Apricot kernel meal is an understudied and underutilized by-product of the apricot industry. In this study, apricot kernel protein (AKP) was obtained from apricot kernel meal by a simple one-step salting-in method, exhibiting high yield (38.4 g AKP/100 g defatted meal), high protein content (91.4%), and high solubility (92.5% at neutral pHs). The possibility and kinetics of AKP fibrillization were further investigated. It was found that apricot kernel protein amyloid fibrils (AKPFs) with twisted nanofibrillar structures were readily formed by heating at pH 2.0 and 90 °C. SDS-PAGE analysis indicated that these AKPFs contained peptide fragments with the molecular weight of around 6.5 kDa. Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD) and intrinsic fluorescence spectroscopy revealed that the structure, morphology and properties of AKPFs were analogous to other food protein-derived amyloid fibrils. Importantly, AKPF has upgraded foaming characteristics, e.g., AKPF-18h (AKPF formed at 18 h) has 31.7% higher foaming capacity and 30.2% higher foam stability relative to AKP. This is attributed to the formation of a homogeneous interfacial film at the air/water interface of the foam. The DPPH radical scavenging activity of AKPF-18h was also improved, reaching 53.0%, about twice that of AKP (28.4%). In addition, the developed AKP and AKPFs were not cytotoxic in vitro. All these findings suggest that AKPFs have great potential for future food applications.