Saturated Zinc Research Articles

Biofortification of iron and zinc under late-season water deficit and iron and zinc deficiency on yield and fatty acid composition of flaxseed

Iron and zinc are essential for plant and human health. Biofortification is crucial for improving crop performance. We studied how biofortification of iron and zinc to flaxseed affected its yield, fatty acid composition, and antioxidant capacity in soil water and nutrient deficiency conditions. We tested nutritional impacts of iron and zinc (0, 4, and 8 g l−1) during the onset of stem elongation (VS) and flowering (RS) within two distinct environments (well-watered (WW) and after-anthesis water deficit (WD), and their interactions, on yield and fatty acid composition of flaxseed in 2018 and 2019 in Kermanshah, Iran. Based on the results in WW, seed, oil, protein, and mucilage yield were 1756, 646, 285, and 131 kg ha−1, and WD reduced seed and oil yield by 24, 33, and significantly increased saturated fatty acids, total phenol, and zinc in the seeds. In WW and WD, iron and zinc fortification significantly increased seed, oil, and protein yield, total phenol, unsaturated fatty acids and iron and zinc in the seeds. Path analysis showed that the number of seed capsule−1 and capsule plant−1 in the WW and WD, respectively, had the most direct and positive impact on seed, oil, mucilage, and protein yield. These findings suggest that oil yield was more sensitive to WD compared to seed, mucilage, and protein yield, and application of iron and zinc can boost the oil quantity and quality, iron, zinc content, and antioxidant capacity by minimizing the harmful impacts of WD.

Nutrient patterns in relation to insulin resistance and endothelial dysfunction in Iranian women.

Prior studies have mainly focused on the association of one specific nutrient with insulin resistance (IR) and endothelial dysfunction and limited studies have assessed the association with different nutrient patterns (NPs). We examined the association between various NPs and IR and endothelial dysfunction among Iranian women. This cross-sectional study was carried out on a sample of 368 female nurses. A 106-items food frequency questionnaire (FFQ) was applied for dietary assessments. Using factor analysis, the relationships between NPs and markers of insulin resistance (HOMA-IR, HOMA-β, and QUICKY), and endothelial dysfunction (E-selectin, sICAM-1, and sVCAM-1) were assessed. Mean age and body mass index of participants were respectively 35.21years and 24.04kg/m2. Three major NPs were identified. NP1, named as "dairy, fruits, and vegetables" had high values of potassium, folate, vitamins A and C, magnesium, and beta carotene. No significant association was observed between this NP and insulin resistance or endothelial dysfunction indices. The second NP was full of chromium, selenium, copper, vitamin B6, monounsaturated fatty acid (MUFA), thiamin, vitamin D, and iron. Adherence to NP2 (named "legumes, nuts, and protein foods") was associated with lower values of insulin (6.8 ± 1.1 versus 8.4 ± 1.1, P = 0.01), homeostasis model assessment-Insulin resistance (HOMA-IR) (1.3 ± 0.2 versus 1.7 ± 0.2, P = 0.02), and vascular adhesion molecule 1 (VCAM-1) (444.2 ± 27.9 versus 475.8 ± 28.4, P = 0.03). However, adherence to the third NP, rich in saturated fatty acid (SFA), cholesterol, sodium, zinc, vitamin E, and B12, described as "animal fat and meat + vitamin E", was associated with higher amounts of homeostasis model assessment-β (HOMA-β) (531.3 ± 176.2 versus 48.7 ± 179.8, P = 0.03). In conclusion, following the NP2, correlated with higher intakes of chromium, selenium, copper, vitamin B6, MUFA and thiamin was associated with lower values of insulin, HOMA-IR, and sVCAM-1. Adherence to NP3, rich in SFA, cholesterol, vitamin E, vitamin B12, and zinc was associated with higher levels of HOMA-β.

A gram-scale synthesis of very-long chain polyunsaturated fatty acids (VLC-PUFAs).

This manuscript describes our third generation, gram-scale synthesis of very long chain-polyunsaturated fatty acids (VLC-PUFAs), a unique and increasingly important class of lipids. Critical to this work and what makes it different from our previous approach to this family was the avoidance of oxidation sequences. Central to accomplishing this involved the use of a Negishi coupling reaction between the acid chloride derived from DHA and a saturated alkyl zinc reaction. Overall, the general approach required 6 synthetic transformations from DHA and was accomplished with an overall yield of 40%.

Dietary habits and dietary nutrient intake in patients with age-related macular degeneration: A case-control study.

Age-related macular degeneration (AMD) is the leading cause of irreversible blindness among older adults in developed countries. Although many risk factors are known, the pathogenesis of AMD is still unclear. However, oxidative stress probably plays a vital role in the process of AMD. The increasing prevalence of AMD, risk of vision loss, limited treatment of dry form, expensive treatment of wet form, and decreased quality of life are factors that lead to considering modifiable risk factors of AMD, such as nutrition. This is the first study describing the relationship between dietary habits, dietary nutrient intake and AMD in the Czech Republic. In this research, a total of 93 cases with AMD and 58 controls without AMD and cataracts participated. All participants were ophthalmologically examined at the Clinic of Eye Treatments at the University Hospital Brno. Data were collected using a pre-tested self-report questionnaire in a face-to-face interview. Food consumption frequency was assessed by an 18-item semiquantitative food-frequency questionnaire (FFQ). Dietary nutrient intakes were calculated from a 24-hour recall. Patients with AMD compared with controls had significantly higher consumption of legumes and lower consumption of meat products, salt and salty products. In men, we found statistically significant differences in alcohol consumption. The case group consumed alcoholic beverages more frequently (median: 2 times a week) than the control group (median: 1-3 times a month). No differences in alcohol consumption were found in women. In comparison to the case group, the control group had a significantly higher dietary intake of energy (5,783.8 vs. 4,849.3 kJ/day; p = 0.002), proteins (65.3 vs. 52.3 g/day; p = 0.002), fats (57.6 vs. 49.4 g/day; p = 0.046), saturated fatty acids (21.7 vs. 18.9 g/day; p = 0.026), carbohydrates (150.4 vs. 127.1 g/day; p = 0.017), dietary fibre (13.2 vs. 11.3 g/day; p = 0.044), vitamin B2 (1.0 vs. 0.9 mg/day; p = 0.029), vitamin B3 (13.9 vs. 10.0 mg/day; p = 0.011), pantothenic acid (3.5 vs. 2.8 mg/day; p = 0.001), vitamin B6 (1.3 vs. 1.0 mg/day; p = 0.001), potassium (1,656.5 vs. 1,418.0 mg/day; p = 0.022), phosphorus (845.4 vs. 718.7 mg/day; p = 0.020), magnesium (176.5 vs. 143.0 mg/day; p = 0.012), copper (1.0 vs. 0.8 mg/day; p = 0.011), and zinc (7.1 vs. 6.1 mg/day; p = 0.012) counted from a 24-hour recall. According to FFQ, dietary habits in the patients with AMD and controls were similar. In men from the case group, we found statistically significant higher alcohol consumption. According to a 24-hour recall, the controls achieved recommended dietary intakes rather than cases. In comparison to the case group, the control group had a significantly higher dietary intake of energy, proteins, fats, saturated fatty acids, carbohydrates, dietary fibre, vitamin B2, vitamin B3, pantothenic acid, vitamin B6, potassium, phosphorus, magnesium, copper, and zinc.

Distribution of Toxocara vitulorum in Cattle of Ağrı Region

Parasitizing in the small intestines of cattle, buffalo and bison, Toxocara vitulorum is seen in countries with humid tropical and subtropical climates, including Türkiye. Adult parasites can cause digestive disorders such as loss of appetite, weakness, diarrhea or constipation, and sometimes death, especially in calves. This study was planned to investigate the distribution of T. vitulorum in cattle of the Ağrı region. In this study, stool samples of the size of a walnut were taken from the rectum of 200 calves 0-6 months old and 200 cattle older than 1 year in the province of Ağrı and its districts, and the preparations were prepared using the saturated zinc sulfate flotation method and the preparations were examined under a light microscope. T. vitulorum eggs were detected in 70 (35%) of 200 calves aged 0-6 months and 21 (10.5%) of 200 cattle over one year old. The difference in positivity between cattle over one year old and calves was statistically significant (p=0.001). With fecal examination ın 22 of the calves (11%), Trichostrongylidae spp. eggs were detected. It was determined that the prevalence of T. vitulorum in calves was high in Diyadin, Eleşkirt, Doğubayazıt, Hamur, Taşlıçay, Tutak and Patnos districts of Ağrı province. We believe that larger-scale studies should be conducted on the spread of this parasite, which causes significant low yields in cattle breeding.

Contamination of Soils with Helminthic Eggs in Some Districts of Duhok Governorate / Kurdistan Region-iraq

Duhok Governorate, Kurdistan Region consists of many districts, where there is a high distribution of animals, presences of public parks and pastures. The present study was conducted in some of districts (Duhok center, Semel, Amadyia and Sharya) of Duhok Governorate, Kurdistan Region. The current study amid to determine the contamination of soils with helminthic ova and their correlation with the district residents, type of soil and presence of animals (Mainly stray Dogs and Cats). During this study, 533 soil samples were collected randomly from October 2021 to May 2022.  The samples were collected from different places such as dog shelters, general parks, groves, Hospital parks, near pastures, near sewage, private gardens, school parks, and near slaughter houses.  The collected samples were tested by flotation technique using saturated zinc sulphate (specific gravity 1.2).    The total rate of soil contamination with helminthic ova in all examined samples was 23.45% (125 / 533). The highest contamination rate with these ova was reported in soil samples examined from Sharya Camp 53.57% (30/56), while the lowest contamination rate was from Semel district 19.22% (49/255). The rates of ova in soils collected from Amadyia and Duhok center were 23.61% (34/144) and 15.38% (12/78), respectively. Regarding to the locations, soil samples examined from rural areas revealed high rate of contamination 29.33% (107/366) as compared with urban areas, in which the rate was 10.78% (18/167). The contamination rates with helminthic ova according to the type of soil, in clay soil was 26.54%, while in silty and sandy soils were 24.39% and 14.96%, respectively. The contamination rate of soils with these ova was higher in areas populated with animals mainly dogs and cats which was 26.44% (78/295) as compared to areas free from animals 19.75% (47/238) (19. Regarding to different places, the results showed that soils in places close to the sewage were highly contaminated with ova 50.00% (3/6), followed by those near pasture 37.50% (15/40), groves 35.56% (16/35), dog shelters 32.18% (28/87), school Parks 29.41% (10/34), near slaughter houses 27.50% (11/40), public places 21.05% (32/152). On the other hand, the lowest contamination of the soil with these ova was recorded in private gardens 8.26% (10/121), while the soil samples from hospital parks were free from parasitic ova. According to the type of helminths, ova of 6 helminthic genera were reported in all (533) examined soil samples, with Toxocara ova being the most prevalent helminths detected in 34 (6.38%), samples, then ova of Hymenolepis nana in 32 (6.00%), Taeniid ova in 26 (4.88%), Ancylostoma ova in 15 (2.81%), Ascaris ova in 12 (2.25%), and Trichuris ova in 6 (1.13%).  In conclusion, the soils of some districts were contaminated with different types of helminthic eggs. Therefore, the people of these districts will be at a high risk of acquiring infection with different species of these parasites when they get contacts with the soil

Microplastics and Microrubbers in Soils Around Two Landfills and a Municipal Solid Waste Transfer Station in Ahvaz Metropolis, Iran

Background: Microplastics (MPs) and microrubbers (MRs) are a substantial source of pollutants entering the environment and a cause for debate in environmental studies in soils surrounding two landfills and a municipal solid waste transfer station near Ahvaz Metropolis. Since the current information about these particles in Iranian municipal solid waste transfer station and landfill systems is scanty, this study aimed to determine the amounts and abundance of MPs and MRs in soils. Methods: Each of the twelve sites that determined using a systematic grid sampling method had approximately 100 g of soil samples from a depth of 0-10 cm with three replications. The method used for extracting MPs from the soil samples was density separation with saturated zinc chloride solution. The particles were investigated by the size, shape, abundance and colour. A total of 1807 MP and 1872.7 MR particles were detected from the samples. The Pearson’s correlation coefficient (r) was used for describing correlations. Results: The highest abundance of MPs was observed at the S5 site (325.9±26.8 items/100 g soil). The particles were categorized into fragments, foams, fibers, films and spheres. Five ranges of particle size were identified (between 1 mm ≤L and L≤100 μm) in nine color categories. The 1 mm ≤L size class was dominant in MPs (54%) and MRs (52%). The majority of the MPs (41.8%) were white/transparent, whereas MRs were identified as black/gray (99.1%). Conclusion: Contamination by MPs and MRs exceeded allowable standards, compared to other transfer stations across the globe.

The effect of strain rate on the Zanjan zinc tailing behavior in monotonic triaxial testing

Tailing is an artificial waste material that is created after the extraction of a metal from mineral soils that seriously need to be investigated in various aspects, including from geotechnical engineering point of view. These silt sized materials are mostly influenced by the strain rate effects. For this, a series of triaxial testings has been perfomed on reconstituted saturated zanjan zinc tailing specimens. The tests were done under two confining pressures of 50 and 200 kPa with a relative density of 73% at four different strain rates of 0.5, 0.3, 0.1 and 0.05 mm/min. The testing results showed that as the strain rate increases 10-fold, the amount of the maximum deviator stress increases 1.90 and 1.33 times on average for CU and CD conditions, respectively. Also in both conditions, the higher the strain rate, the higher the friction angle. Moreover, the results showed that with increasing the strain rate, the values of pore water pressure decreased by 0.6 times. By reducing the strain rate 10 times, the volumetric strain increases 3 times on average. It was concluded that the strain rate has effective influence on the shear behavior of the zanjan zinc tailing.

Consumption of Free Sugar Predicts Nutrient Intake of Saudi Children.

Data concerning the association between free sugar intake and nutrient intake in children in the Middle East are not available. This study aimed to explore the association between the consumption of free sugar and nutrient intake among Saudi children. A cross-sectional study included 424 Saudi children ages between 6 and 12 years old and their mothers. An online survey collected sociodemographic data from mothers. Trained data collector personal contacted mothers to arrange for a phone interview in order to collect 24-h dietary recall to assess the dietary intake of children. Only 0.90% of children consumed free sugar within the recent recommendation of the World Health Organization (WHO) of <5% of total energy intake, whereas 10.6% of children consumed free sugar <10% of total energy intake. The percentage of free sugar intake was negatively associated with total energy intake. Multiple linear regression analysis of free sugar consumption and nutrient intake adjusted for children's age and sex indicated that a higher percentage of free sugar intake significantly predicted lower intake of saturated fat, fiber, sodium, potassium, calcium, iron, zinc, and vitamin B12. Excessive intake of free sugar predicted a lower intake of many essential nutrients. Interventions that aim to reduce the intake of free sugar are urgently needed in order to improve diet of growing children. Future research to explore top food sources of free sugar is needed to establish effective interventions that aim to limit free sugar intake among children.

Validation of Global Diet Quality Score Among Nonpregnant Women of Reproductive Age in India: Findings from the Andhra Pradesh Children and Parents Study (APCAPS) and the Indian Migration Study (IMS)

BackgroundIn India, there is a need to monitor population-level trends in changes in diet quality in relation to both undernutrition and noncommunicable diseases. ObjectivesWe conducted a study to validate a novel diet quality score in southern India. MethodsWe included data from 3041 nonpregnant women of reproductive age (15–49 years) from 2 studies in India. Diet was assessed using a validated food frequency questionnaire (FFQ). The Global Diet Quality Score (GDQS) was calculated from 25 food groups (16 healthy; 9 unhealthy), with points for each group based on the frequency and quantity of items consumed in each group. We used Spearman correlations to examine correlations between the GDQS and several nutrient intakes of concern. We examined associations between the GDQS [overall, healthy (GDQS+), and unhealthy (GDQS−) submetrics] and overall nutrient adequacy, micro- and macronutrients, body mass index (BMI), midupper arm circumference, hemoglobin, blood pressure, high density lipoprotein (HDL), and total cholesterol (TC). ResultsThe mean GDQS was 23 points (SD, 3.6; maximum, 46.5). In energy-adjusted models, positive associations were found between the overall GDQS and GDQS+ and intakes of calcium, fiber, folate, iron, monounsaturated fatty acid (MUFA), protein, polyunsaturated fatty acid (PUFA), saturated fatty acid (SFA), total fat, and zinc (ρ = 0.12–0.39; P < 0.001). Quintile analyses showed that the GDQS was associated with better nutrient adequacy. At the same time, the GDQS was associated with higher TC, lower HDL, and higher BMI. We found no associations between the GDQS and hypertension. ConclusionsThe GDQS was a useful tool for reflecting overall nutrient adequacy and some lipid measures. Future studies are needed to refine the GDQS for populations who consume large amounts of unhealthy foods, like refined grains, along with healthy foods included in the GDQS.

Improving the sensitivity of gastrointestinal helminth detection using the Mini-FLOTAC technique in wild birds.

High-performance-validated tests are essential for successful epidemiological monitoring, surveillance of parasitic infections, and comparative studies in wildlife populations. The Mini-FLOTAC is a novel flotation-based technique for the sensitive detection and quantification of gastrointestinal parasites that is recently being explored for use in wildlife. A limitation of any flotation-based copromicroscopic method is the selection of the flotation solution (FS), which might influence the performance of the test. However, no study has compared the influence of using different FS in the Mini-FLOTAC technique for parasite detection in wild birds. Here, we evaluated the diagnostic performance of the Mini-FLOTAC in three waterbird host species using two widely used FS: saturated salt (NaCl; specific gravity 1.20) and saturated zinc sulfate (ZnSO4; specific gravity 1.35). One hundred fresh fecal samples were analyzed for parasite fecal egg counts (FEC). Regardless of the host species, fecal samples evaluated with the Mini-FLOTAC method using ZnSO4 resulted in a significantly higher detection rate and higher FEC of strongylid, capillarid, cestode, and trematode parasites, than samples analyzed with the NaCl solution. Our concise study demonstrated the importance of using an appropriate FS for the identification of parasite eggs in wildlife species, especially in hosts with an expected aggregated distribution and low parasite load such as waterbird hosts. The higher analytical sensitivity of the Mini-FLOTAC technique achieved with ZnSO4, and its applicability to fieldwork, highlights this method as a promising tool for the quantitative surveillance of parasite infections in wild bird populations.

Evaluation of Nutrient Intake and Food Consumption among Dutch Toddlers.

Improving dietary habits at a young age could prevent adverse health outcomes. The aim was to gain insight into the adequacy of the dietary intake of Dutch toddlers, which may provide valuable information for preventive measures. Data obtained from the Dutch National Food Consumption Survey 2012–2016 were used, which included 672 children aged one to three years. Habitual intakes of nutrients were evaluated according to recommendations set by the Dutch Health Council. Specific food groups were evaluated according to the Dutch food-based dietary guidelines. For most nutrients, intakes were estimated to be adequate. High intakes were found for saturated fatty acids, retinol, iodine, copper, zinc, and sodium. No statement could be provided on the adequacy of intakes of alpha-linoleic acids, N-3 fish fatty acids, fiber, and iron. 74% of the toddlers used dietary supplements, and 59% used vitamin D supplements specifically. Total median intakes of vegetables, bread, and milk products were sufficient. Consumption of bread, potatoes and cereals, milk products, fats, and drinks consisted largely of unhealthy products. Consumption of unfavorable products may have been the cause of the observed high and low intakes of several nutrients. Shifting towards a healthier diet that is more in line with the guidelines may positively affect the dietary intake of Dutch toddlers and prevent negative health impacts, also later in life.

An examination of contributions of animal- and plant-based dietary patterns on the nutrient quality of diets of adult Canadians.

Dietary guidance and Canada's 2019 Food Guide encourage increased consumption of plant-based foods as a source of dietary protein. However, there is an absence of recent data on protein and nutrient intakes and quality of Canadian dietary patterns that might occur with increased plant protein intakes. This study compared food sources and nutrient intakes of Canadian adults within groups of increasing plant protein-containing diets. The CCHS 2015 Public-Use Microdata File of single 24-hour dietary recalls of males and females ≥19years (n= 6498) or ≥70years (n= 1482) were examined. Respondents were allocated into 4 groups defined by their protein intake percentage coming from plant-based foods (i.e., group 1: 0-24.9%, group 2: 25-49.9%, group 3: 50-74.9%, group 4: 75-100%). Protein intake in adults averaged 63.3% animal and 36.7% plant protein. Where plant protein contributed >50% protein, higher intakes of carbohydrate, dietary fibre, folate, dietary folate equivalents, iron and magnesium (p< 0.001) but lower intakes of total and saturated fat, protein, vitamin D, vitamin B12, riboflavin and niacin (p< 0.0001) were reported. In contrast, group 1 had higher total and saturated fat, protein, vitamin B12, thiamin, niacin, and zinc, but lower carbohydrate, dietary fibre, and magnesium. Balancing plant- with animal-based protein foods leads to healthier dietary patterns with more favourable nutritional properties when compared with diets based on either high animal or high plant protein content. Novelty: Combinations of animal- and plant-based proteins improve nutrient quality of Canadian diets. The source of protein influences diet quality.

Association of Nutrient Patterns and Their Relation with Obesity in Iranian Adults: a Population Based Study.

In the present research, we have evaluated the association between patterns of nutrient intake and obesity. The present cross-sectional study recruited 850 adults aged between 20–59 years old. Dietary intakes were assessed with three 24-hour recalls. As well, data on anthropometric measures were collected. General obesity was specified as body mass index ≥ 30 kg/m2. Factor analysis was conducted, and followed by a varimax rotation, was performed to extract major nutrient patterns. Our analysis identified three major nutrient patterns: The first nutrient pattern was characterized by the high consumption of saturated fatty acids (SFAs), protein, vitamins B1, B2, B6, B5, B3, B12, Zinc, and iron. The second nutrient pattern was rich in total fat, polyunsaturated fatty acids, monounsaturated fatty acids, SFAs, oleic acid, linolenic acid, zinc, vitamin E, α-tocopherol, and β-carotene. The third one was greatly loaded with protein, carbohydrate, potassium, magnesium, phosphorus, calcium, vitamin C, and folate. Women in the third quintile of the first pattern were less likely to be generally obese in the fully adjusted model (odds ratio, 0.44; 95% confidence interval, 0.25–0.75). None of the other nutrient patterns had a significant association with obesity, even after adjusting for confounders. Adherence to a nutrient pattern rich in water-soluble vitamins was significantly associated with a greater chance of general obesity among women. Further studies in other populations, along with future prospective studies, are required to confirm these findings.

Independent variation in copper tolerance and copper accumulation among crop species and varieties

Copper (Cu) locally contaminates soils and might negatively affect growth and yield of crops. A better understanding of plant copper tolerance and accumulation is needed in order to breed more Cu-tolerant or Cu-efficient crops. Cu tolerance was evaluated in different varieties of seven species (Brassica carinata, B. juncea, B. napus, Cynara cardunculus, Helianthus annuus, Nicotiana tabacum and Raphanus sativus) exposed to a series of CuSO4 concentrations (0.1–8 μM CuSO4) in the nutrient solution. Plants were further exposed to 0.1 μM CuSO4 and to their variety-specific concentrations that reduced root growth to 50% of the maximum rate (EC50). Among all the varieties of all the species the EC50 varied from 0.7 up to 3.1 μM Cu. B. carinata was significantly more Cu-sensitive than the other species, which were not significantly different among each other, and B. carinata and H. annuus accommodated significant intra-specific, inter-varietal variation. There were significant differences between species in Cu uptake efficiency and nutrient status. When under EC50 exposure, all the Brassicaceae, except B. carinata, maintained low Cu concentrations in shoots, whereas the other species and B. carinata exhibited significantly increased shoot Cu concentrations, compared to the control. There was no apparent relationship between Cu tolerance and Cu accumulation in roots and shoots, suggesting that the observed variation in tolerance, both between and within species, is not explained by differential exclusion capacity. Discriminant analysis and treatment comparisons suggest possible contribution of lignin, saturated fatty acids, manganese (Mn) and zinc (Zn) in tolerance to high Cu concentrations in shoot.

Assessing risk factors associated with prevalence of canine gastrointestinal parasitic zoonoses in Andhra Pradesh, India

Prevalence and risk factors associated with gastrointestinal parasites in dogs from central coastal districts of Andhra Pradesh, India was estimated by coprological examination. On examination of faecal samples of 1,907 dogs by saturated zinc sulphate floatation, sedimentation technique and modified Ziehl-Nielsen staining, an overall prevalence of 52.2% was observed. Single species infections were more common (49.7%) than mixed infections (2.5%). A total of 11 different species were detected. The most prevalent species was Ancylostoma canis followed by Toxocara canis, Cystoisospora ohioensis, Dipylidium caninum, Toxascaris leonina, Capillaria aerophila, Diphyllobothrium latum, Cystoisospora canis, Entamoeba spp., Trichuris vulpis and Cryptosporidium spp. The overall prevalence was high in stray dogs (65.9%). The prevalence of A. caninum (31.6%) was significant in pet dogs. Significant relationship between the season and prevalence of parasites was observed, the infection being more prevalent during rainy season. Binary logistic regression analysis identified age, season and the living condition of dogs and lack of zoonotic awareness of owners as risk factors for different parasitic infection.

The Role of House Fly Musca domestica in transmitting the Intestinal Parasites for Human

The present study is made up during October 2014 till March 2015 to know the parasitic stages which are carried out by different part Musca domestica. The samples are collected from five different places which are included the restaurants, schools , kitchens, grid sewage and wild land. The number of the samples which are examined is reached 100 samples, all of them were examined by direct smear using the physiological solution and lugal iodine solution by flotation way and by using saturated zinc sulfide solution and diagnosed four species of intestinal parasites which are Entamoeba coli, Entamoeba histolytic, Giardia lamblia ,Trichomonas hominess .

Chickens bred extensively as sentinels from soil contamination by Toxocara

Environmental contamination by Toxocara spp eggs can be verified by parasitological techniques, which are mostly laborious, have low sensitivity, and may require repeated tests to establish the contamination status of a certain area. Given the significance of this parasite as an agent of infections in humans, that may cause blindness, especially in children, broilers chickens created extensively were evaluated as possible markers of parasite environmental contamination, through the detection of anti-Toxocara serum antibodies. Eighteen 15-day-old Label Rouge broilers were released on a farm with a history of dogs with Toxocara infection. At 0, 15 and 30 days after the release birds blood samples were taken, as well as soil samples. At the end of the experiment the birds were slaughtered, and the gastrointestinal tract was collected for coprological exams. The presence of anti-Toxocara antibodies resulted in increased reactivity indexes with time, and at 15 days five of the birds were positive and at 30 days, seven birds showed seroconversion. Examination of the soil samples by the centrifugation-flotation method in hyper saturated zinc sulfate solution revealed contamination by embryonated or infertile eggs of Toxocara at all times of collection, as well as the presence of Ascaridia eggs at 15 days after release of birds. Examination of bird's stool samples at the end of the experiment demonstrated 100% infection by Ascaridia galli, however there was no correlation between the counts of this parasite and the ELISA reactivity indices for anti-Toxocara antibodies. The results obtained allow us to infer the possibility of using anti-Toxocara spp-specific antibodies determination in birds raised extensively, which could then serve as sentinels of environmental contamination by this parasite.

INTESTINAL NEMATODES IN DOGS AND CATS IN BANJA LUKA, REPUBLIKA SRPSKA

Some intestinal nematodes of dogs and cats show zoonotic potential, and therefore theyare of public-health significance. In the territory of the city of Banja Luka, 149 samples wereexamined (131 of dogs and 18 cats), with 40 samples of dog feces collected from two public areasin the city. Parasitological examination (saturated zinc sulfate floatation and determination of larvaeand adult parasites in feces) resulted in the following data: Toxocara canis (9.16%),Ancylostomatidae (5.34%), Trichuris vulpis (7.63%), Toxascaris leonina (1.52%), Toxocara cati(33.3%), Trichuris spp. (5.5%) and Hydatigera taeniaeformis (5.5%). On the basis of the obtainedresults we can conclude that the intestinal nematodes are more widespread than is generallyassumed and therefore represent a health problem for dogs and cats in the territory of Banja Luka.

The prevalence of intestinal parasites of domestic cats and dogs in Vladivostok, Russia during 2014–2017

ABSTRACTThe prevalence of intestinal parasites was determined in 144 dogs and 135 cats in Vladivostok, Russia during four years of study. Association between general animal characteristics, such as age and sex, and infections was investigated. The parasitological examination of faecal samples from the dogs and cats was performed by means of the flotation technique using saturated zinc sulfate solution and sedimentation technique. Intestinal parasites were found in 32 (22.1%) of the 144 dogs studied. In total, six different intestinal parasites were identified: Ancylostoma caninum in ten dogs (6.9%), Cystoisospora sp. in seven dogs (4.9%), Uncinaria stenocephala in four dogs (2.8%), Taenia sp. in two dogs (1.3%), Toxocara canis in three dogs (2.1%) and Dipylidium caninum in six dogs (4.2%). Intestinal parasites were found in 25 (18.5%) of the 135 cats studied. In total, six different intestinal parasites were identified: Cystoisospora sp. in eight cats (13.5%), D. caninum in three cats (2.2%), Opisthorchii...