Sampling Campaigns Research Articles

Are Membrane Bioreactors Really More Efficient in Removing Pharmaceutical Substances?—Variance Component Analysis Of Micropollutant Removal

This study evaluates the influence of micropollutant chemical characteristics on the removal of pharmaceutical substances through three different treatments: membrane bioreactor, full wastewater treatment with final filtration (WWTP), and secondary treatment through a conventional activated sludges system, operated in parallel at realistic sludge retention time (SRT) over three years and four sampling campaigns. Treated wastewater from the WWTP enters the local canal with a low dilution ratio. Therefore, the monitoring of water contamination is of particular interest for a reliable assessment of environmental risk. A total of 39 pharmaceutical substances were screened. While differences among the three types of treatments were found statistically not significant, data analysis performed through a generalized linear model showed that both the influent concentration and the physicochemical characteristics are strong predictors for the removal of micropollutant. SRT had no significance for the three types of treatment of this study. Finally, pharmaceuticals were divided into three major classes based on their influent concentration and removal. A canonical discriminant analysis was used to predict the removals and showed that the pharmaceuticals removal rates are strongly influenced by their hydrophobicity/hydrophilicity and enabled to predict their removal categories with high accuracy (i.e., 65% of correct predictions).

Iron redox shuttling and uptake by silicate minerals on the Namibian mud belt

Anoxic marine sediments represent an important source of bioavailable iron (Fe) to the ocean. The highest sedimentary Fe fluxes are observed in open-marine oxygen minimum zones where anoxic bottom waters are in contact with ferruginous surface sediments. Here, sedimentary Fe release, transport and re-deposition (i.e., Fe shuttling) may generate a lateral pattern of sedimentary Fe enrichment and depletion, which can be used to trace redox-related Fe mobility in the paleo-record. However, depending on the balance between terrigenous and authigenic (i.e., shuttle-related) Fe flux, the stability of bottom water redox conditions as well as post-depositional processes of mineral alteration, the sedimentary fingerprint of an Fe redox shuttle may be obscured and difficult to identify.We investigated sedimentary Fe cycling along two transects across the Namibian mud belt with variable terrigenous sedimentation (23°S < 25°S) and during two seasons with opposing bottom water redox conditions (oxic in austral winter versus anoxic to sulfidic in austral summer). On both transects, substantial benthic Fe fluxes up to −50 µmol m−2 d−1 were inferred based on pore water profiles. The magnitude of these fluxes is comparable to those reported for other open-marine oxygen minimum zones. On the transect at 23°S, Fe source areas with ferruginous surface sediments were clearly separated from Fe sink areas with highly sulfidic surface sediments. Therefore, Fe redox shuttling was reflected by a lateral pattern of reactive Fe depletion and enrichment relative to the terrigenous background sedimentation. By contrast, on the transect at 25°S, benthic Fe fluxes were temporally and spatially more variable and surface sediments were ferruginous or only weakly sulfidic. Therefore, sedimentary Fe depletion and enrichment was less pronounced at 25°S. In the Fe sink area at 23°S, hydrogen sulfide was present at the sediment surface during both sampling campaigns and solid phase data suggest that Fe sulfide minerals represented the main burial phase of reactive Fe. By contrast, at 25°S excess Fe was associated with potassium (K) rather than reduced sulfur. While a differing sediment provenance cannot be ruled out entirely, combined evidence from pore water silica profiles, K to Fe stoichiometric relationships and electron microprobe images suggest that laterally derived excess Fe was incorporated into pre-existing and/or authigenic clay minerals during early diagenesis. Iron uptake by clay minerals may be supported by frequent redox oscillations and sediment mixing preventing preservation of Fe sulfide minerals and promoting Fe and K fixation in clay minerals. The burial fluxes of excess Fe associated with sulfide minerals at 23°S and silicate minerals at 25°S were similar. Our findings thus underscore that neoformation or alteration of silicate minerals can be important processes within the low-temperature marine Fe cycle.

The Role Sampling Campaign: Opportunities for Utilizing Natural Landscapes to Fulfill Microhydro-Based Renewable Energy in Remote Area

Abstract West Java, as a province with a low electrification ratio, especially in remote areas, faces significant challenges. Recent data shows that around 77,626 households (34.91%) do not yet have access to electricity. Nonetheless, the southern region of West Java has great potential thanks to its diverse natural morphology. These landscape characteristics open up opportunities to utilize renewable energy sources, such as micro-hydro potential, to meet electricity needs. The objectives of this study include (1) Priority Election Needs of Area of Interest (AOI); (2) Sampling Campaign: Micro Hydro Opportunities in AOI. The research method used was survey research with a mixed approach. Data collection techniques include field data documentation; geospatial analysis and interviews. Data analysis was descriptive statistics. The results showed that (1) Garut Regency (3.76%) is a priority location with Singajaya sub-district (14.34%) and Sukamulya village (34.32%); (2) Sukamulya Village has potential micro hydro energy opportunities at Tiis waterfall at the foot of Mount Lumbung, the results of a rough assessment of this location can provide electrical energy to surrounding family groups. The implication of this research output can be a relevant reference for the local government to be followed up in the future action plan.

Carbon-14 cycling in a nuclearized river: A first study in the downstream part of the Rhône River (France)

Carbon-14 (14C) has a natural origin but is also anthropogenically released from civil nuclear facilities. Due to its long decay period (half-life: 5700 ± 30 years), it is a persistent radionuclide in the environment. In rivers, the complex speciation of carbon makes the fate of industrial 14C difficult to track. This study reports a first overview of artificial 14C cycling in a nuclearized river. A one-year sampling campaign was conducted on the French nuclearized Rhône River and two of its non-nuclearized tributaries (Durance and Ardèche rivers). Isotopic (δ13C, Δ14C) and carbon concentrations analyses were performed on the particulate organic carbon (POC), dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC). Chlorophyll-a (Chl-a) and tritium analyses were performed to assess the dynamic of aquatic organic matter and the nuclear industry contribution, respectively. Comparisons of Δ14C data obtained from the Rhône River with those from the tributaries highlight significant industrial radiocarbon labelling in all carbon forms, with medians of 142, 130 and 42 ‰ for POC, DOC and DIC, that are 2–3 times higher than those of the tributaries. The high values of Chl-a/POC ratios with Δ14C-enriched POC suggest a biological uptake of artificial Δ14C in DIC by aquatic photosynthesis. The relationship of Δ14C-DIC with tritium activity indicates a response to recent releases and enables the contribution of nuclear power plants to be estimated at a median of 26 %. Sampling at the Rhône's mouth would reinforce our understanding of the fate of riverine 14C when entering the marine environment.

Antineoplastic drugs in healthcare settings: Occupational exposure and risk graduation

Antineoplastic drugs (ADs) are hazardous medicinal products highlighted in the EU strategic framework on health and safety at work 2021–2027. To minimize workers' chronic exposure in oncologic settings, regular monitoring programs for these drugs are crucial and mandatory in the EU (Directive 2004/37/EC). No surveillance exists in Portugal, thus we intended to assess environmental contamination and to perform occupational exposure and risk graduation in a Portuguese tertiary hospital. Wipe sampling and liquid chromatography–tandem mass spectrometry were employed to analyze thirteen drugs of concern (bicalutamide, capecitabine, cyclophosphamide, cyproterone, doxorubicin, etoposide, flutamide, ifosfamide, imatinib, megestrol, mycophenolate mofetil, paclitaxel, prednisone), eight for the first time worldwide, in 152 surface samples over three sampling campaigns. Surface contamination before general cleaning (worst-case scenario) was higher and more widespread in the pharmacy (84%–98 % positive samples) than in the day-care hospital (40%–72 %). No samples were found above the “action limit” (10000 pg/cm2), but concentrations were frequently above the “safe”/alert level (100 pg/cm2), particularly for cyclophosphamide and ifosfamide (largest campaign's 90th percentiles: 2197 pg/cm2 and 1898 pg/cm2). Despite the approach's nuances, the maximum daily dermal intake calculated (1.6 μg/day, considering a single genotoxic drug) was lower than the acceptable daily intake. This study provided knowledge regarding surface contamination in a European hospital, where occupational exposure to some ADs of concern cannot be ruled out and follow-up monitoring is recommended, even if workers seem unlikely to develop cancer from handling them. Furthermore, this work contributes to the implementation of regular environmental monitoring programs for ADs in Portugal, enhancing compliance with EU recommendations.

Natural Attenuation Potential of Vinyl Chloride and Butyl Acrylate Released in the East Palestine, Ohio Train Derailment Accident.

The East Palestine, Ohio train derailment released toxic vinyl chloride (VC) and butyl acrylate (BA), which entered the watershed. Streambed sediment, surface water, and private well water samples were collected 128 and 276 days postaccident to assess the natural attenuation potential of VC and BA by quantifying biodegradation biomarker genes and conducting microcosm treatability studies. qPCR detected the aerobic VC degradation biomarkers etnC in ∼40% and etnE in ∼27% of sediments collected in both sampling campaigns in abundances reaching 105 gene copies g-1. The 16S rRNA genes of organohalide-respiring Dehalococcoides and Dehalogenimonas were, respectively, detected in 50 and 64% of sediment samples collected 128 days postaccident and in 63 and 88% of sediment samples collected 276 days postaccident, in abundances reaching 107 cells g-1. Elevated detection frequencies of VC degradation biomarker genes were measured immediately downstream of the accident site (i.e., Sulphur Run). Aerobic VC degradation occurred in all sediment microcosms and coincided with increases of etnC/etnE genes and Mycobacterium, a genus comprising aerobic VC degraders. The conversion of VC to ethene and an increased abundance of VC reductive dechlorination biomarker genes were observed in microcosms established with sediments collected from Sulphur Run. All anoxic microcosms rapidly degraded BA to innocuous products with intermediate formation of n-butanol and acrylate. The results indicate that microbiomes in the East Palestine watershed have natural attenuation capacity for VC and BA. Recommendations are made to improve first-response actions in future contaminant release accidents of this magnitude.

O short-branch Microsporidia, where art thou? Identifying diversity hotspots for future sampling

Short-branch Microsporidia were previously shown to form a basal grade within the expanded Microsporidia clade and to branch near the classical, long-branch Microsporidia. Although they share simpler versions of some morphological characteristics, they do not show accelerated evolutionary rates, making them ideal candidates to study the evolutionary trajectories that have led to long-branch microsporidian unique characteristics. However, most sequences assigned to the short-branch Microsporidia are undescribed, novel environmental lineages for which the identification requires knowledge of where they can be found. To direct future isolation, we used the EukBank database of the global UniEuk initiative that contains the majority of the publicly available environmental V4 SSU rRNA gene sequences of protists. The curated OTU table and corresponding metadata were used to evaluate the occurrence of short-branch Microsporidia across freshwater, hypersaline, marine benthic, marine pelagic, and terrestrial environments. Presence–absence analyses infer that short-branch Microsporidia are most abundant in freshwater and terrestrial environments, and alpha- and beta-diversity measures indicate that focusing our sampling effort on these two environments would cover a large part of their overall diversity. These results can be used to coordinate future isolation and sampling campaigns to better understand the enigmatic evolution of microsporidians’ unique characteristics.

Fate and transport of ten plant protection products of emerging concern in a coastal lagoon: Application and evaluation of a multimedia level III fugacity model

Multimedia fugacity models are effective tools for studying the environmental behaviour and occurrence of contaminants of emerging concern (CECs) and assessing associated risks, especially when experimental data is limited. These models describe processes controlling chemical partitioning, transport, and reactions in environmental media using mathematical statements based on the concept of fugacity.To aid in identifying and prioritizing CECs for future local monitoring, we present here the application of a level III multimedia fugacity model assuming non-equilibrium between compartments and steady-state conditions. This model estimated predicted environmental concentrations (PECs), persistence, distribution, and transport of ten plant protection products (PPPs) in the Venice Lagoon, a complex coastal environment under high anthropogenic pressure.The model was evaluated through uncertainty and sensitivity analysis using the Monte Carlo approach and by comparing PECs with PPP concentrations measured during four sampling campaigns. Results showed good agreement with field data, with the highest concentrations in water and sediments estimated for glyphosate, followed by imidacloprid, metaflumizone, and triallate. The model indicated accumulation of all investigated PPPs in sediments. For most chemicals, advection outflow and degradation in the water column were the main removal mechanisms, while volatilization was significant only for oxadiazon and triallate.Sensitivity and uncertainty analysis revealed that degradation rates, organic carbon/water partitioning coefficients (KOC), and parameters describing air-water interactions had the strongest influence on the model's results, followed by inputs accounting for sediment sinking and resuspension. The lack of data on PPP degradation in brackish waters accounted for most of the uncertainty in model results.This work shows how a relatively simple multimedia model can offer new insights into the environmental behaviour of PPPs in a complex transitional waterbody such as the Venice lagoon, providing useful data for the identification of the CECs to be prioritised in future local monitoring efforts.

Atmospheric concentrations of per- and polyfluoroalkyl substances and their emissions at a waste recycling facility producing refuse-derived paper and plastics densified fuel

To better understand the types and concentrations of per- and polyfluoroalkyl substances (PFAS) emitted into the air from waste recycling facilities that produce refuse-derived paper and plastics densified fuel (RPF) from industrial waste, we conducted an air sampling campaign at a waste recycling facility in Japan. Both passive and active air sampling were conducted, and the samples collected were used to quantify the PFAS emitted into the air during the production of RPF. Overall, few ionic PFAS were detected in the air at the facility; however, high levels of neutral PFAS (8.21–53.4 ng/m3; 20.7–130 pmol/m3) were measured in the air near the heat molding machines. The two neutral PFAS detected at the highest concentrations were 6:2 fluorotelomer alcohol and 6:2 fluorotelomer methacrylate, which are currently unregulated under the Stockholm Convention, suggesting that product manufacturers have shifted away from using regulated PFAS. Small amounts of regulated PFAS such as 8:2 fluorotelomer methacrylate and 8:2 fluorotelomer acrylate were measured in some parts of the facility. Analysis of the concentrations of PFAS in the exhaust gas from the heat molding machines revealed neutral PFAS concentrations (537–2160 ng/m3; 1350–5040 pmol/m3) that were 1–2 orders of magnitude higher than those in the surrounding indoor air. The total emission of neutral PFAS from the facility to the environment was estimated to be 0.066–0.260 g/day (0.168–0.607 mmol/day), depending on whether air volume discharged as exhaust gas or as indoor ventilation was considered. A contribution analysis of the emissions revealed that treating the exhaust gas from the heat molding machines, which constitutes over 94 % of the total emissions, is very effective at reducing PFAS emissions from the facility.

Phytoplankton community composition as a driver of annual autochthonous organic carbon dynamics in the northern coastal Baltic Sea

Phytoplankton are the major primary producers in the pelagic system. They greatly influence biogeochemical cycles but little is known about the importance of shifting phytoplankton community composition for carbon dynamics. This study investigates the impact of seasonal changes in coastal phytoplankton communities on pelagic carbon fluxes. A field sampling campaign, covering an annual cycle in primary production, was conducted to assess the seasonal changes of phytoplankton communities and relevant organic carbon parameters in the coastal Baltic Sea. The monitoring frequency ranged from 1 to 3 wk, adapted to match the seasonal phytoplankton blooms. In addition, sediment traps were deployed to determine the particulate carbon and nutrient export to the seafloor in every season. We found that the phytoplankton biomass during the spring bloom was as high as 550 µg C l-1 and was dominated by diatom species (88% of total phytoplankton biomass). In comparison, the more species-rich summer bloom reached a combined maximum biomass of 236 µg C l-1. However, the highest export flux of particulate organic carbon was found in the middle of August (561 mmol C m-2 d-1) and, not as expected, around the spring bloom in May (226 mmol C m-2 d-1), suggesting a high potential for carbon recycling within the pelagic food web rather than being exported to the seafloor or advected laterally. Our study emphasizes the importance of keystone species and diversity for carbon transport processes in marine coastal ecosystems and highlights complex relationships between phytoplankton biomass production, community composition and carbon dynamics.

Potential applications of microbial genomics in nuclear non-proliferation.

As nuclear technology evolves in response to increased demand for diversification and decarbonization of the energy sector, new and innovative approaches are needed to effectively identify and deter the proliferation of nuclear arms, while ensuring safe development of global nuclear energy resources. Preventing the use of nuclear material and technology for unsanctioned development of nuclear weapons has been a long-standing challenge for the International Atomic Energy Agency and signatories of the Treaty on the Non-Proliferation of Nuclear Weapons. Environmental swipe sampling has proven to be an effective technique for characterizing clandestine proliferation activities within and around known locations of nuclear facilities and sites. However, limited tools and techniques exist for detecting nuclear proliferation in unknown locations beyond the boundaries of declared nuclear fuel cycle facilities, representing a critical gap in non-proliferation safeguards. Microbiomes, defined as "characteristic communities of microorganisms" found in specific habitats with distinct physical and chemical properties, can provide valuable information about the conditions and activities occurring in the surrounding environment. Microorganisms are known to inhabit radionuclide-contaminated sites, spent nuclear fuel storage pools, and cooling systems of water-cooled nuclear reactors, where they can cause radionuclide migration and corrosion of critical structures. Microbial transformation of radionuclides is a well-established process that has been documented in numerous field and laboratory studies. These studies helped to identify key bacterial taxa and microbially-mediated processes that directly and indirectly control the transformation, mobility, and fate of radionuclides in the environment. Expanding on this work, other studies have used microbial genomics integrated with machine learning models to successfully monitor and predict the occurrence of heavy metals, radionuclides, and other process wastes in the environment, indicating the potential role of nuclear activities in shaping microbial community structure and function. Results of this previous body of work suggest fundamental geochemical-microbial interactions occurring at nuclear fuel cycle facilities could give rise to microbiomes that are characteristic of nuclear activities. These microbiomes could provide valuable information for monitoring nuclear fuel cycle facilities, planning environmental sampling campaigns, and developing biosensor technology for the detection of undisclosed fuel cycle activities and proliferation concerns.

Ecological patterns of benthic foraminiferal communities driven by seasonal and spatial environmental gradients in an Arctic fjord

AbstractArctic fjords, being transitional areas between glacier‐covered land and the ocean, are characterized by strong environmental gradients. The seasonal melting of glaciers generates strong turbidity and primary production antagonist gradients, which can affect benthic habitats. Two sampling campaigns were carried out in Kongsfjorden (Svalbard, Arctic Ocean) in May and August 2021 to investigate seasonal changes in benthic foraminifera spatial distribution and ecosystem functioning along a longitudinal transect of 10 km from the Kronebreen tidewater glacier front. Concurrently, organic matter quantity and biochemical composition, sediment grain size, and physical parameters of the water masses were investigated as possible driving factors of benthic ecosystem responses. In a previous study, three statistically determined foraminiferal biozonations (glacier proximal, medial, and distal) were observed on the basis of their species content within the closest 10 km from the glacier front, presenting similar assemblages in both seasons. Our results indicate that foraminiferal distribution at the local scale is mainly driven by physical and geochemical gradients induced by melting waters and sediment discharges from the tidewater glacier occurring during summer. Due to the climate change, the melting season is expected to last longer and increasing global temperature will much probably accelerate the melting processes. Our findings strongly support the use of foraminifera as bioindicators to monitor the effects of ongoing climate change on the benthic ecosystems of Arctic fjords and, accessorily, as proxies for reconstructing glacier front positions in the recent past.

Let the dust settle: Impact of enhanced rock weathering on soil biological, physical, and geochemical fertility

Terrestrial enhanced rock weathering (ERW) is a promising carbon dioxide removal technology that consists in applying ground silicate rock such as basalt on agricultural soils. On top of carbon sequestration, ERW has the potential to raise the soil pH and release nutrients, thereby improving soil fertility. Despite these possible co-benefits, concerns such as heavy metal pollution or soil structure damage have also been raised. To our knowledge, these contrasted potential effects of ERW on soil fertility have not yet been simultaneously investigated. This field trial aimed at assessing the impact of ERW on biological, physical, and chemical soil properties in a temperate agricultural context. To do so, three vineyard fields in Switzerland were selected for their distinct geochemical properties and were amended with basaltic rock powder at a dose of 20 tons per hectare (2 kg.m−2). On each field, basaltic rock powder was either applied one year before the sampling campaign, one month before the sampling campaign, or not applied (control) for a total of 27 plots with 9 repetitions of each level. Overall, basaltic rock powder addition had a predominantly positive to neutral effect on soil fertility. Most soil properties showed no significant change either 1 month or 1 year post application. Nevertheless, our study highlighted a significant increase in earthworm abundance (+71 % on average), soil respiration (+50 %) and extractable sodium concentration (+23 %) as early as 1 month post application. The higher soil respiration raises the question of CO2 losses from organic matter mineralization that could limit ERW's efficiency. The increase in sodium raises concerns about a sodification risk potentially damaging soil fertility. These elements now require further investigation before enhanced rock weathering can be considered a viable and secure carbon dioxide removal technology.

Characteristics and Indicative Significance of Groundwater Stable Isotopes in the Loess Plateau at the Regional Scale

Regional groundwater recharge is a critical scientific issue for sustainable groundwater resource development and management. However, spatial variations in groundwater recharge in the Loess Plateau （LP） remain poorly understood. To fill this knowledge gap, a systematic sampling campaign and stable isotope analysis were carried out for groundwater （shallow aquifer） in 13 major catchments during July 2019. The main objectives of this study were： ① to understandthe spatial distribution and influencing factors of stable isotopes in groundwater and ② to reveal the groundwater recharge sources and pathways and their spatial variations, combined with the precipitation stable isotope datasets. Stable isotopes in groundwater had poor spatial variations at the regional scale； however, they became isotopically depleted with the increase in annual average precipitation on the catchment scale （r = -0.87）. Compared with the stable isotope of precipitation, stable isotopes of groundwater were generally depleted and were similar to the precipitation of the rainy season （July-September）. These together indicated that there was pronounced seasonality of groundwater recharge, and the main recharge period was the rainy season. In particular, the recharge seasonality index （δP/G） was closely related to the catchment's average annual precipitation （r = -0.77） and leaf area index （r = -0.63）. In addition, groundwater lc-excess was generally negative, with the catchment-mean value ranging from -4.3‰ to -0.7‰. Hydrologically, this indicated that groundwater recharge pathways （ratio of matrix flow vs. preferential flow） were different among these catchments, which should be quantitatively determined by combining the saturated zone （groundwater） and the unsaturated zone （soil） in future work. Our findings can improve the understanding of groundwater recharge in LP and provide a scientific basis for sustainable management of groundwater resources at the regional scale.

High-frequency spatial sediment source fingerprinting using in situ absorbance data

Sediment fingerprinting is a commonly applied approach to quantify catchment suspended sediment (SS) source contributions. However, due to the high workloads and costs involved in SS sampling and laboratory analyses, traditional procedures often result in a limited number of samples and sampling campaigns, which can hamper the capacity to capture the intra- and inter-storm variations in SS source activation and deactivation. A better understanding of the temporal dynamics in source contributions is needed, however, to improve the understanding of dynamic sediment generation and delivery processes for selecting management practices. In recognition of the extant need to improve high-frequency source estimates and to permit long duration measurement campaigns, the work reported herein presents a sediment fingerprinting procedure using UV–Vis absorbance spectra measured in situ with submersible spectrophotometer probes to estimate spatial SS source contributions based on a confluence-based monitoring strategy. The high-frequency (5-minute intervals) measurements were used to fingerprint SS sources during seven selected periods with varying hydrological conditions. Source apportionment estimates (MixSIAR model) were validated using an independent sediment budget. The results showed a mean deviation of 9.4 ± 6.0 %, compared with the sediment budget, for the seven periods combined (n = 7,282 time steps). The most accurate results (mean absolute error of 5.3 ± 4.3 %) were obtained for the period covering the July 2021 flood event in central Europe (return period for the monitored catchment > 20 years). Overall, modelled variations in SS source contributions mostly followed the sediment budget dynamics. This novel work highlights the potential for using spectrophotometer absorbance data for high-frequency and long-term sediment fingerprinting purposes. We argue that the presented method can lead to new opportunities to improve our understanding of SS sources and their dynamics.

Fingerprinting of emerging contaminants in L'Albufera natural park (Valencia, Spain): Implications for wetland ecosystem health

Wetlands are crucial ecosystems that are increasingly threatened by anthropogenic activities. L'Albufera Natural Park, the second-largest coastal wetland in Spain, faces significant pressures from surrounding agricultural lands, industrial activities, human settlements, and associated infrastructures, including treated wastewater inputs. This study aimed at (i) establishing pathways of emerging pollutants entering the natural wetland using both target and non-target screening (NTS) for management purposes, (ii) distinguishing specific contamination hotspots through Geographic Information System (GIS) and (iii) performing basic ecological risk assessment to evaluate ecosystem health. Two sampling campaigns were conducted in the spring and summer of 2019, coinciding with the start and end of the rice cultivation season, the region's primary agricultural activity. Each campaign involved the collection of 51 samples. High-resolution mass spectrometry (HRMS) was employed, using a simultaneous NTS approach with optimized gradients for pesticides and moderately polar compounds, along with complementary NTS methods for polar compounds, to identify additional contaminants of emerging concern (CECs). Quantitative analysis revealed that fungicides comprised a substantial portion of detected CECs, constituting approximately 50% of the total quantified pesticides. Tebuconazole emerged as the predominant fungicide, with the highest mean concentration (>16.9 μg L−1), followed by azoxystrobin and tricyclazole. NTS tentatively identified 16 pesticides, 43 pharmaceuticals and personal care products (PPCPs), 24 industrial compounds, and 12 other CECs with high confidence levels. Spatial distribution analysis demonstrated significant contamination predominantly in the southwestern region of the park, gradually diminishing towards the north-eastern outlet. The composition of contaminants varied between water and sediment samples, with pharmaceuticals predominating in water and industrial compounds in sediments. Risk assessment, evaluated through risk quotient calculations based on parent compound concentrations, revealed a decreasing trend towards the outlet, suggesting wetland degradation capacity. However, significant risk levels persist throughout much of the Natural Park, highlighting the urgent need for mitigation measures to safeguard the integrity of this vital ecosystem.

Soil Carbon Dioxide Emissions and Carbon Sequestration with Implementation of Alley Cropping in a Mediterranean Citrus Orchard.

Agroecological ecosystems produce significant carbon dioxide fluxes; however, the equilibrium of their carbon sequestration, as well as emission rates, faces considerable uncertainties. Therefore, sustainable cropping practices represent a unique opportunity for carbon sequestration, compensating greenhouse gas emissions. In this research, we evaluated the short-term effect of different management practices in alleys (tillage, no tillage, alley cropping with Rosmarinus officinalis and Thymus hyemalis on soil properties, carbon sequestration, and CO2 emissions in a grapefruit orchard under semiarid climate). For two years every four months, soil sampling campaigns were performed, soil CO2 emissions were measured, and rhizosphere soils were sampled at the end of the experimental period. The results show that alley cropping with Thymus and Rosmarinus contributed to improve soil fertility, increasing soil organic carbon (SOC), total nitrogen, cation exchange capacity, and nutrients. The CO2 emission rates followed the soil temperature/moisture pattern. Tillage did not contribute to higher overall CO2 emissions, and there were no decreased SOC contents. In contrast, alley crops increased CO2 emission rates, especially Rosmarinus; however, the bigger root system and biomass of Rosmarinus contributed to soil carbon sequestration at a greater rate than Thymus. Therefore, Rosmarinus is positioned as a better option than Thymus to be used as an alley crop, although long-term monitoring is required to evaluate if the reported short-term benefits are maintained over time.

Assessment of chemical contamination by cancer drugs during use of the RIVATM compounding robot: A pilot study.

Many hospitals are now investing in robotic compounding system for the preparation of cytotoxic agents. The objective of the present study was to describe contamination by cytotoxics inside and outside the RIVATM robot (ARxIUM, Winnipeg, Canada). We applied a risk analysis to determine which locations inside and outside the compounding robot should be monitored. Samples were collected by swabbing with a wet swab (using 0.1 mL of sterile water) before the robots was cleaned. Ten cytotoxics compounded with the robot were screened for using LC-MS/MS. We determined the percentage contamination rates inside (CRin) and outside (CRout) the robot and the amounts of each contaminant (in ng/cm²). If a sample was found to be positive, a corrective action was implemented. Our risk analysis highlighted 10 locations inside the robot and 7 outside. Ten sampling campaigns (10 samples per campaign) were performed. The mean CRin (40%) was significantly higher than the mean CRout (2%; p < 10-4). Gemcitabine and cyclophosphamide were the main contaminants. After the implementation of corrective measures (such as daily cleaning with SDS/isopropyl alcohol), the CRin fell from 60% to 10%. The frequency of contamination was lower for robotic compounding than for manual compounding in an isolator. However, robotic compounding tended to generated larger mean amounts of contaminant; this was related to incidents such as splashing when syringes were disposed of after the compounding. The implementation of corrective actions effectively reduced the CRs. Further longer-term studies are required to confirm these results.

Characterization and source apportionment of pharmaceuticals in surface water of the Yangtze Estuary and adjacent sea.

Pharmaceuticals, which are closely linked to human activities, have attracted global attention. This study investigated the occurrence characteristics of 20 pharmaceuticals in surface water of the Yangtze Estuary and adjacent sea. A total of 14 targeted pharmaceuticals were detected in both spring and summer sampling campaigns. The mean concentrations of sulfonamides and non-sulfonamides were 36.60 ± 19.43ng·L-1 and 50.02 ± 41.07ng·L-1, respectively. As for non-antibiotics, their concentrations were in the range of 24.34 ± 916.8ng·L-1 with caffeine accounting for 6.17 ~ 86.70% (average percentage of 42.22%). Meanwhile, spatial distribution patterns showed similarities between antibiotics and non-antibiotics, with high levels occurring near the upper estuary, aquaculture areas, wastewater treatment plants, and the maximum turbidity zone. This phenomenon could be related to the sources of pharmaceuticals and the physicochemical properties of water bodies. Obviously, the first three areas are highly impacted by human activities or serve as important sources of terrestrial contaminants entering the East China Sea. The last area retains high amounts of suspended particles which may exert strong trapping effects on hydrophobic chemicals. Principal component analysis revealed the presence of three potential sources for pharmaceuticals in the Yangtze Estuary, with a relatively high percentage originating from incompletely treated municipal sewage. As for the temporal trend, pharmaceutical contamination was found to be higher in spring compared to summer, potentially due to variations in pharmaceutical consumption patterns, local rainfalls, and water temperatures. These findings provide fundamental data support for implementing appropriate local management strategies for pharmaceutical usages.

Source apportionment of particle-bound polycyclic aromatic hydrocarbons (PAHs), oxygenated PAHs (OPAHs), and their associated long-term health risks in a major European city

Many studies have drawn attention to the associations of oxygenated polycyclic aromatic hydrocarbons (OPAHs) with harmful health effects, advocating for their systematic monitoring alongside simple PAHs to better understand the aerosol carcinogenic potential in urban areas. To address this need, this study conducted an extensive PM2.5 sampling campaign in Athens, Greece, at the Thissio Supersite of the National Observatory of Athens, from December 2018 to July 2021, aiming to characterize the levels and variability of polycyclic aromatic compounds (PACs), perform source apportionment, and assess health risk. Cumulative OPAH concentrations (Σ-OPAHs) were in the same range as Σ-PAHs (annual average 4.2 and 5.6 ng m−3, respectively). They exhibited a common seasonal profile with enhanced levels during the heating seasons, primarily attributed to residential wood burning (RWB). The episodic impact of biomass burning was also observed during a peri-urban wildfire event in May 2021, when PAH and OPAH concentrations increased by a factor of three compared to the monthly average. The study period also included the winter 2020–2021 COVID-19 lockdown, during which PAH and OPAH levels decreased by >50 % compared to past winters. Positive matrix factorization (PMF) source apportionment, based on a carbonaceous aerosol speciation dataset, identified PAC sources related to RWB, local traffic (gasoline vehicles) and urban traffic (including diesel emissions), as well as an impact of regional organic aerosol. Despite its seasonal character, RWB accounted for nearly half of Σ-PAH and over two-thirds of Σ-OPAH concentrations. Using the estimated source profiles and contributions, the source-specific carcinogenic potency of the studied PACs was calculated, revealing that almost 50 % was related to RWB. These findings underscore the urgent need to regulate domestic biomass burning at a European level, which can provide concrete benefits for improving urban air quality, towards the new stricter EU standards, and reducing long-term health effects.