Rye Samples Research Articles

Development of an LC-MS/MS Determination Method for T-2 Toxin and Its Glucoside and Acetyl Derivatives for Estimating the Contamination of Total T-2 Toxins in Staple Flours.

A determination method previously validated for trichothecenes and zearalenone by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was adapted for the quantification of T-2 toxin (T-2) as well as its glucoside and acetyl derivatives, T-2-3-glucoside (T-2-3G) and 3-acetyl-T-2 (3A-T-2). HT-2 toxin (HT-2) and its acetyl derivative 3-acetyl-HT-2 (3A-HT-2) were also included as the target chemicals. Staple flours (56 samples collected from the Singapore market) were examined for contamination from T-2 and/or HT-2 and their derivatives. Among them, 16 flours were found to be contaminated with T-2 and/or HT-2, whereas none was contaminated with T-2-3G and 3A-HT-2, except for trace 3A-T-2 detected in 2 rye samples. Rye flour samples were frequently contaminated with both T-2 and HT-2. Some of the reference materials (RMs) were further analyzed, and T-2-3G and 3A-T-2 were quantitatively detected in corn and wheat RMs. The ratio of T-2-3G to T-2 in the RMs seemed to be much lower than the ratio of deoxynivalenol-3-glucoside to deoxynivalenol usually reported in former studies. To the best of our knowledge, the natural contamination of 3A-T-2 in staple flour is reported here for the first time.

Anthocyanin Composition and Content in Rye Plants with Different Grain Color.

The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1...vi6) lead to the absence of anthocyanins in all parts of the plant. Moreover, dominant genes of anthocyanin synthesis in aleurone (gene C) and pericarp (gene Vs) also affect the color of the grain. Reverse phase high-performance liquid chromatography and mass spectrometry were used to study anthocyanins in 24 rye samples. A lack of anthocyanins in the lines with yellow and brown grain was determined. Delphinidin rutinoside and cyanidin rutinoside were found in the green-seeded lines. Six samples with violet grains significantly varied in terms of anthocyanin composition and content. However, the main aglycone was cyanidin or peonidin in all of them. Monosaccharide glucose and disaccharide rutinose served as the glycoside units. Violet-seeded accession forms differ in the ratio of the main anthocyanins and the range of their acylated derivatives. The acyl groups were presented mainly by radicals of malonic and sinapic acids. For the colored forms, a profile of the revealed anthocyanins with the indication of their contents was given. The obtained results are discussed in connection to similar data in rice, barley, and wheat, which will provide a perspective for future investigations.

Chlortetracycline and related tetracyclines: detection in wheat and rye grain.

Antibiotic drugs are excreted to a large proportion by livestock. Thus, antibiotics are distributed on fields with slurry and can be taken up by plants. In the present study, hydroponic experiments were performed to reveal whether the widely administered chlortetracycline is taken up into wheat grain in a concentration-dependent manner. A further goal was to determine (chlor)tetracyclines in wheat and rye grain from agricultural practice. Increasing chlortetracycline deposition in wheat grain was observed with a rising chlortetracycline spiking level in the hydroponic solution. In 371 selected wheat and rye samples from three growing years of agricultural practice, the overall detection frequency was 21% for tetracyclines. In the most highly contaminated sample, tetracyclines occurred at 18.2 μg kg-1 . Tetracycline residues were also found in rye grain. Conversion and degradation products of (chlor)tetracycline such as tetracycline, doxycycline and demeclocycline were detected in grains from hydroponic experiments and from agricultural practice. Concentrations of tetracyclines found in wheat and rye grains were of no concern with respect to toxicity regarding human consumption. However, antibiotic concentrations below the minimum inhibitory concentration can select for antibiotic resistance in bacteria. Thus, low levels of different tetracycline residues contained in food should be taken into account regarding risk assessment. © 2018 Society of Chemical Industry.

Microwave pretreatment allows accurate fatty acid analysis of small fresh weight (100 g) dried alfalfa, ryegrass, and winter rye samples

Accurate analysis of forage fatty acid (FA) profile and content, particularly polyunsaturated FA (PUFA), such as alpha-linolenic acid (ALA) and linoleic acid (LA), is vital for ongoing research optimizing the desired FA content of ruminant-derived foods. Lyophilization (i.e., freeze-drying) is regarded as the gold standard for preserving labile constituents, such as PUFA, in fresh forage samples. This method, however, is expensive, time consuming, and generally impractical for the large number and size of forage samples in agronomic studies.The objective of the first experiment was to evaluate the efficacy of a brief (1 min) microwave pretreatment prior to forced hot air drying (FHA) for preserving alfalfa (Medicago sativa L.) and Italian ryegrass (Lolium multiflorum L.) forage samples, relative to both a freeze-drying control and FHA drying alone. In a second experiment, similar drying methods were examined in winter rye (Secale cereale L.), as well as the decline of ALA proportion of total FA in ground samples stored 72 weeks.In the first experiment, small fresh weight samples (100 g) receiving 1 min microwave pretreatment before FHA drying were found equivalent to freeze-dried samples in FA content and profile, and both were greater in ΣPUFA than samples that received FHA alone. Large fresh weight samples (400–500 g) receiving FHA alone, a common practice in agronomic studies, contained 1.5–2.5 fold lower total ALA content and 1.3–1.6 fold lower LA content than the freeze-dried control, while large fresh weight samples (400–500 g) receiving 1 or 2 min microwave pretreatment contained 1.2–2.2 fold lower total ALA content and 1.1–1.5 fold lower LA content than the freeze-dried control. The second experiment revealed a reduction in ALA proportion of total FA of stored samples over a long period of time regardless of preparation method; however, FHA samples had a greater rate of reduction.This study confirms that FHA drying alone is not adequate for forage sample preservation for FA analysis, and may lead to a significant underestimation of ALA, the principal FA in plants. This distorts measurements of other FA reported on a proportional basis (g 100 g−1 total FA). In conclusion, a simple and inexpensive 1 min microwave pretreatment prior to FHA drying results in FA analysis comparable to freeze-dried samples, provided that samples are of a small fresh weight (100 g).

Aflatoxins and ochratoxin A in flour: a survey of the Serbian retail market

ABSTRACTThis report presents data on the occurrence of aflatoxins (AF) and ochratoxin A in different types of flour marketed in Serbia. A total of 114 samples of wheat, buckwheat, rye, oat, barley, rice, millet and corn flour were collected in the period 2012–2016 and analysed using high performance liquid chromatography with fluorescence detection. Among flours other than corn, AFB1 was quantified only in rice, while ochratoxin A (OTA) was found in 29% of the samples. In corn flours the percentage of positive samples varied greatly over the years: AFB1 7.1–80.0%, OTA 30.0–40.6%, with a co-occurrence of 7.1–34.4%. Overall 5.2% of flours other than corn and 10.7% of corn flours exceeded the maximum levels (MLs) for AFB1 and/or OTA. The highest recorded levels were 8.80 μg kg−1 of AFB1 (corn) and 23.04 μg kg−1 of OTA (rye). Overall mean contamination levels of corn flours were 0.53 μg kg−1 of AFB1 and 0.46 μg kg−1 of OTA.

Flavonoids, anthocyanins, phenolamides, benzoxazinoids, lignans and alkylresorcinols in rye (Secale cereale) and some rye products

Besides dietary fibre, rye (Secale cereale) grains are rich source of various phytochemicals such as phenolic acids, lignans and alkylresorcinols. The major aim of this study was to gain new insight into certain less studied phytochemicals e.g. flavonoids, anthocyanins, phenolamides and benzoxazinoids in wholegrain rye and its milling fractions as well as in selected rye products by ultra performance liquid chromatography combined with quadrupole time-of-flight mass spectrometery (UPLC-QTOF). In addition, quantitation of total flavonoid content, anthocyanins, major benzoxazinoids and alkylresorcinols in the rye samples was accomplished by high performance liquid chromatography with a diode array detector (HPLC-DAD). Although the concentration of flavonoids in rye was low compared to, e.g. berries and vegetables, the chemical diversity of flavonoids was high. Various phenolamides were also tentatively identified from rye and rye samples. High concentrations of benzoxazinoids were found in rye malt flour, coarsely ground rye malts and the traditional Finnish dish, mämmi.

Role of Sample Processing Strategies at the European Union National Reference Laboratories (NRLs) Concerning the Analysis of Pesticide Residues.

The guidance document SANTE 11945/2015 recommends that cereal samples be milled to a particle size preferably smaller than 1.0 mm and that extensive heating of the samples should be avoided. The aim of the present study was therefore to investigate the differences in milling procedures, obtained particle size distributions, and the resulting pesticide residue recovery when cereal samples were milled at the European Union National Reference Laboratories (NRLs) with their routine milling procedures. A total of 23 NRLs participated in the study. The oat and rye samples milled by each NRL were sent to the European Union Reference Laboratory on Cereals and Feedingstuff (EURL) for the determination of the particle size distribution and pesticide residue recovery. The results showed that the NRLs used several different brands and types of mills. Large variations in the particle size distributions and pesticide extraction efficiencies were observed even between samples milled by the same type of mill.

Level of contamination with mycobiota and contents of mycotoxins from the group of trichothecenes in grain of wheat , oats, barley, rye and triticale harvested in Poland in 2006- 2008.

The risk of cereal exposure to microbial contamination is high and possible at any time, starting from the period of plant vegetation, through harvest, up to the processing, storage and transport of the final product. Contents of mycotoxins in grain are inseparably connected with the presence of fungal biomass, the presence of which may indicate the occurrence of a fungus, and indirectly also products of its metabolism. Analyses were conducted on 378 grain samples of wheat, triticale, barley, rye and oats collected from grain silos located at grain purchase stations and at mills in Poland in 2006, 2007 and 2008. The concentrations of ERG and mycotoxins from the group of trichothecenes, as well as CFU numbers were analysed. The tested cereals were characterised by similarly low concentrations of both the investigated fungal metabolites and the level of microscopic fungi. However, conducted statistical analyses showed significant variation between tested treatments. Oat and rye grain contained the highest amounts of ERG, total toxins and CFU. In turn, the lowest values of investigated parameters were found in grain of wheat and triticale. Chemometric analyses, based on the results of chemical and microbiological tests, showed slight differences between contents of analysed metabolites between the years of the study, and do not confirm the observations on the significance of the effect of weather conditions on the development of mycobiota and production of mycotoxins; however, it does pertain to treatments showing no significant infestation. Highly significant correlations between contents of trichothecenes and ERG concentration (higher than in the case of the correlation of the total toxin concentrations/log cfu/g), indicate that the level of this metabolite is inseparably connected with mycotoxin contents in grain.

The Application of FT-IR Spectroscopy for Quality Control of Flours Obtained from Polish Producers.

Samples of wheat, spelt, rye, and triticale flours produced by different Polish mills were studied by both classic chemical methods and FT-IR MIR spectroscopy. An attempt was made to statistically correlate FT-IR spectral data with reference data with regard to content of various components, for example, proteins, fats, ash, and fatty acids as well as properties such as moisture, falling number, and energetic value. This correlation resulted in calibrated and validated statistical models for versatile evaluation of unknown flour samples. The calibration data set was used to construct calibration models with use of the CSR and the PLS with the leave one-out, cross-validation techniques. The calibrated models were validated with a validation data set. The results obtained confirmed that application of statistical models based on MIR spectral data is a robust, accurate, precise, rapid, inexpensive, and convenient methodology for determination of flour characteristics, as well as for detection of content of selected flour ingredients. The obtained models' characteristics were as follows: R2 = 0.97, PRESS = 2.14; R2 = 0.96, PRESS = 0.69; R2 = 0.95, PRESS = 1.27; R2 = 0.94, PRESS = 0.76, for content of proteins, lipids, ash, and moisture level, respectively. Best results of CSR models were obtained for protein, ash, and crude fat (R2 = 0.86; 0.82; and 0.78, resp.).

Simple method for estimating soil mass loading onto plant surface using magnetic material content as a soil indicator – Influence of soil adhesion to vegetation on radioactive cesium concentration in forage

A simple technique for estimating soil mass loading on vegetation was developed using magnetic material content as an indicator of soil adhesion. Magnetic material contents in plant and soil samples were determined by a magnetic analyzer. High recovery rates of 85–97% were achieved in a recovery test in which additional soil was added to powdered plant materials [stem of forage corn (Zea mays L.), aboveground part of Italian ryegrass (Lolium multiflorum Lam.)] at addition rates of 12.3–200 g dry soil kg−1 dry plant material including soil. Samples of different Japanese cultivated soils were tested and showed a range of magnetic contents of 1.27–16.1 g kg−1 on a dry weight basis. These levels are considered adequate for determining soil contamination in plant materials. Then, we applied this method for confirming the effect of soil adhesion on radioactive cesium concentrations in plant samples obtained at the area affected by the 2011 nuclear accident in Japan. The mean soil mass loading (±standard deviation) on forage rye (Secale cereale L.) showing mild lodging was 0.8 ± 0.6 g kg−1, but was 7.4 ± 5.0 g kg−1 for plants with serious lodging. No soil loading was detected on rye plants that showed no lodging. Radioactive cesium concentrations in the rye samples increased linearly with the increase in soil mass loading caused by plant lodging, and consequently mean radioactive cesium concentration for rye plants with serious lodging was about 2.7 times higher than that with no lodging. Cesium radioactivity in forage was affected by variations in soil mass loading onto forage plants caused by changes in plant growth and differences between plant species.

Evolutionary relationships in the genus Secale revealed by DArTseq DNA polymorphism

Till this day, there is not much known about the phylogeny of the Secale genus; therefore, in our research, we tried to shed some lights on the issue of rye (Secale genus) taxonomy. The genetic diversity and phylogenetic relationships were evaluated using 13,842 DArTseq™ polymorphic markers. The model-based clustering (STRUCTURE software) separated our 84 samples into three main clusters: perennial cluster, annual cluster, and S. sylvestre cluster. The same result was obtained using Neighbour Joining tree and self-organizing maps. Secale sylvestre, S. strictum, and S. cereale are the three main species of the Secale genus. Three samples of rye are in basal positions in phylogenetic trees. These accessions share ancient morphological characters and are probably the ancestors of different lineages within Secale. Annual Secale taxa, with the exception of S. sylvestre, create one mutual taxon. We have found out that the semi-perennial taxa of S. cereale var. multicaule and S. strictum subsp. ciliatoglume are genetically closest to the annual species of S. cereale. Phylogenetic signals for semi-perennial and annual taxa are also present in S. strictum subsp. africanum. SNP-based analysis revealed that evolution of annual S. cereale has already begun in S. strictum subsp. africanum. The results showed that S. vavilovii cannot be considered as a separate species but a subspecies of S. cereale Secale cereale subsp. dighoricum is a hybrid. It is still not clear whether we can consider S. strictum subsp. strictum and S. strictum subsp. kuprijanovii as two separate species.

Enzymatic activity of fungi isolated from crops.

AimTo detect and assess the activity of extracellular hydrolytic enzymes and to find differences in enzymograms between fungi isolated from wheat and rye samples and grown on Czapek-Dox Broth and Sabouraud Dextrose Broth enriched with cereal (wheat or rye). Isolated strains were also classified in the scale of biosafety levels (BSL).Material and methodsThe study used 23 strains of fungi cultured from samples of wheat and rye (grain, grain dust obtained during threshing and soil) collected in the Lublin region (eastern Poland). API ZYM test (bioMérieux) was carried out according to the manufacturer’s instructions. Classification of BSL (Biosafety levels) was based on the current literature.ResultsHigh enzymatic activity was found in strains cultured in media containing 1% of wheat grain (Bipolaris holmi, Penicillium decumbens) and with an addition of 1% of rye grain (Cladosporium herbarum, Aspergillus versicolor, Alternaria alternata). The total number of enzymes varied depending on the type of media, and in most cases it was higher in the culture where an addition of cereal grains was used.ConclusionsIsolated strains of fungi reveal differences in the profiles of the enzyme assay. It can be assumed that the substrate enriched in grains stimulate the higher activity of mold enzymes.

Obsah fuz�riov�ch mykotox�nov ekologicky a konvencne pestovan�ch obiln�n na Slovensku

Many species of Fusariumfungi can produce a number of different mycotoxins including trichothecenes such as deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and others. The farming system have an effect on quality of grown plants, including mycotoxin contamination. The contamination of winter wheat (Triticum aestivumL.) and rye grain by toxins with focus on the genus Fusarium was monitored within the years 2009 –2011 under the official control according to EC Regulation 401/2006 and 178/2010 on the territory of the Slovak Republic in organic and conventional grains. The concentration of deoxynivalenol (DON) was determined by HPLC/DAD detector and concentration of zearalenone (ZEA) by HPLC/FLD detector. Results of analysed samples showed that DON was the most prevalent Fusarium toxin. In the analysed samples were observed significant differences between organic and conventional wheat samples. Results showed that samples from organic production contained significantly lower contamination of DON and ZEA mycotoxins than from conventional production. In four wheat samples from conventional production was the content of DON over the EC Regulation no. 1881/2006 about setting the maximum levels for certain contaminants in foodstuff. Within the observed period, the highest DON concentrations were observed in the year 2010, in both wheat and rye samples, and organic and conventional production. The year 2010 was characterised by constant rain, moist soil on the most area of Slovakia. Since lower contamination of Fusarium toxins was found in wheat and rye from organic farming, it can be concluded, that factors related to agricultural practices in this system can reduce the risk of Fusarium mycotoxin contamination.

Rye seeds endurance for storage under model experiment

The aim and tasks of the study. Determination of the optimal seed storage conditions for rye seed samples after monitoring results under model experiment.Materials and methods. The material for the study were samples of seeds of rye: sterility maintainers L.1201 B L.961358 B L.90691 B L.120337 B; hybrid populations: Koroleva, Kharkiv'yanka and sterile line L.1201 A, L.90691A. Seeds were dried to moisture content 5, 6, 7% and kept in conditions of model experiment "accelerated aging" at 37 °C during 12 months. Monitoring of seed viability was performed after 4, 8 and 12 months of storage by seed germination test and length of seedlings.Results and discussions. In model terms "artificial aging for 12 months" conducted monitoring of the seed lines and varieties of rye with a moisture content of 5-7% in terms of vigor, germination and seedling length. The low humidity conditions can be traced decisive influence genotype than the variation of the moisture content in the investigated range.Seed germination after 4, 8, 12 months of seed storage under modeling conditions have lower indexes for lines L.1201 A, L.90691 A, compared with the majority of sterility maintainers and rye varieties. The length of the seedlings doesn’t always image the influence of storage conditions on the population status of seeds. There was advantages of the effect of genotype on seed moisture level in the range of 5-7% for the life of theConclusions. The most of the samples of rye in the conditions of model experiment show the benefits of endurance to store seeds with moisture content 5 and 6 %. There was a lower performance storage capacity for sterile lines L.1201 A, L.90691 A than majority- sterility maintainers and hybrids of rye. Seedling length does not always demonstrate the effect of storage conditions influence on the state of populations seeds.

Sensitive determination of citrinin based on molecular imprinted electrochemical sensor

In this report, a novel molecular imprinted voltammetric sensor based on glassy carbon electrode (GCE) modified with platinum nanoparticles (PtNPs) involved in a polyoxometalate (H3PW12O40, POM) functionalized reduced graphene oxide (rGO) was prepared for the determination of citrinin (CIT). The developed surfaces were characterized by using scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) method. CIT imprinted GCE was prepared via electropolymerization process of 80.0mM pyrrole as monomer in the presence of phosphate buffer solution (pH 6.0) containing 20.0mM CIT. The linearity range and the detection limit of the developed method were calculated as 1.0×10−12–1.0×10−10M and 2.0×10−13M, respectively. In addition, the voltammetric sensor was applied to rye samples. The stability and selectivity of the voltammetric sensor were also reported.

Molecular characterization of Bipolaris sorokiniana populations from winter cereals

Isolates of B. sorokiniana were obtained from infected leaf samples of bread wheat, durum wheat, barley, triticale and rye leaves collected randomly from Gurdaspur, Ludhiana and Ferozepur areas of Punjab. Based on qualitative colony parameters, 30 isolates were selected for further studies. Molecular characterization of the isolates was done using 30 RAPD primers and Polymorphic Information Content values for these ranged from 0.51 to 0.98 with an average of 0.72. A total of 197 alleles were amplified out of which 184 were polymorphic and 13 were monomorphic. The number of alleles amplified varied from 3-13 and size of amplified fragments varied from 3.5 Kb to 200bp.The similarity index values ranged from 0.48 to 0.78 indicating wide range of genetic diversity among isolates. On cluster analysis of the molecular data, the isolates were grouped into two major clusters at 50 per cent level of similarity, whereas at 60 per cent similarity coefficient, the isolates were grouped into 10 clusters. Five complete clusters were formed by 25 isolates whereas 5 isolates formed independent lineages. RAPD profiles, however, did not correlate polymorphism with the geographic source or host source of the isolates.

An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC – hybrid quadrupole time-of-flight mass spectrometry

An ultra-high performance liquid chromatography hybrid quadrupole – time of flight (Q-TOF) mass spectrometry (MS) method is described for the simultaneous quantitative determination of common ergot alkaloids and the screening, detection and identification of unexpected (less studied or novel) members of this class of toxic fungal secondary metabolites. The employed analytical strategy involves an untargeted data acquisition (consisting of full scan TOF MS survey and information dependent acquisition MS/MS scans) and the processing of data using both targeted and untargeted approaches. Method performance characteristics for the quantitative analysis of 6 common ergot alkaloids i.e. ergometrine, ergosine, ergotamine, ergocornine, ergocristine, ergokryptine and their corresponding epimers in rye were comparable to those previously reported for triple-quadrupole (QqQ) MS/MS. The method limits of quantification (LOQ) were in the range from 3 to 19 μg/kg, and good linearity was observed for the different ergot alkaloids in the range from LOQ to 1000 μg/kg. Furthermore, the method demonstrated good precision (relative standard deviations at 50 μg/kg not higher than 14.6 and 16.2% for the intra-day and inter-day precision, respectively), and the trueness values at different concentration levels were all between 89 and 115%. The method was applied for the analysis of a set of 17 rye samples and demonstrated the presence of these ergot alkaloids in the range from <LOQ to 2,811 μg/kg. Further mining of the same data based on a ‘non-targeted peak finding’ algorithm and the use of full MS and MS/MS accurate mass data allowed the detection and identification of 19 ergot alkaloids that are commonly not included in most analytical methods using QqQ instruments. Some of these alkaloids are reported for the first time in naturally contaminated samples.

The study of microspore morphogenesis activity In Vitro in anther culture of rye

The morphogenetic capacity of rye microspores in anther culture was investigated. Testing the influence of 2 inductive nutrient medium (190-2 and W14) for passing the first stage of androgenesis in vitro at anther culture of 4 samples of rye were conducted. It is shown that both nutrient media suitable for formation of new growth in anther culture of rye of Ukrainian selection. The five albino regenerates were obtained

Health Risks Associated with Exposure to Fungi

Abstract Occupational environment of farmers is full of biohazards. Especially frequent are molds found in grain, grain dust, and soil. People might be affected by diseases caused by these molds inducing: skin infections, bronchial asthma, allergic rhinitis and others. The aim of the study was to detect and quantify colonization by molds in samples of grain and grain dust collected during combine threshing, as well as in soil samples from organic and conventional cultivation systems in south – east Poland. The following were used for the study: 20 samples of rye, wheat and barley grain, 20 samples of every grain dust collected during combine threshing and 20 samples of soil for every cereal were collected from an organic cultivation system. The same number of corresponding samples from a conventional system of farming was also obtained. The concentrations (CFU/g) of molds were determined applying the method of plate dilution on two media, and the species composition of molds was determined using taxonomic keys and atlases. The results show a potential risk for people engaged in agriculture. The presence of allergenic and toxigenic molds in the work environment of this working group creates serious occupational exposure. This subject is of high significance for the health of population engaged in agricultural production and calls for further studies.

Adaptive peculiarities of winter rye samples productivity and its elements

Purpose. Determination of differential assessment, adaptive capacity and stability of experimental rye F1 hybrids and their parental components.Methods. Estimation of rye F1 hybrids and their parental components was performed by the following indices: productivity, productive tillering, plant height, main spike length, grain number per plant, grain weight and number from the main spike, and 1000-grain weight. The investigations were carried out in 2011-2013 on experimental field of the Plant Production Institute nd. a V.Ya. Yuryev in the Laboratory of Winter Rye Breeding and Genetics. Eight paternal components, two sterile parent lines, and 16 experimental hybrids were used in the TopCross system.Results. The assessment of the adaptive capacity of the experimental hybrids and parental components of winter rye demonstrated a correlation of quantitative traits. Samples with high overall adaptive capacity, stability and breeding value were isolated. Basing on the study results a new winter rye F1 hybrid Saturn combining high overall adaptive capacity by all the studied traits with high winter hardiness and increased starch content in grain in its genotype was transferred to the state trials.