Salvia Injection Research Articles

Influence of compound salvia injection on oxidative stress and complications in patients with gestational hypertension

Objective To investigate the influence of compound salvia injection on oxidative stress and complications in patients with gestational hypertension. Methods From August 2015 to Fabuary 2017, 140 patients with gestational hypertension in Binzhou Central Hospital were selected as study objects, and randomly divided into control group and research group according to the digital table, with 70 cases in each group.The control group was treated with routine treatment, the research group was given routine treatment combined with compound salvia injection.After treatment, SOD, MDA, NO, HMGB1, Cys C, hs-CRP, TNF-α, blood pressure and complications between the two groups were compared. Results After treatment, the levels of NO[(942.58±80.69)mmol/L vs.(734.51±78.63)mmol/L] and SOD[(124.65±23.81)U/mL vs.(90.64±24.13)U/mL] in the research group were higher than those in the control group, and the MAD level[(10.47±3.14)nmol/L vs.(15.82±3.32)nmol/L]in the research group was lower than that in the control group, the difference were statistically significant between the two groups(t=15.451, 8.394, 9.795, all P<0.05). After treatment, the levels of HMGB1[(1.81±0.43)mg/L vs.(3.89±0.65)mg/L], Cys C[(1.04±0.18)mg/L vs.(1.42±0.27)mg/L], TNF-α[(15.09±3.14)ng/L vs.(21.45±4.78)ng/L] and hs-CRP[(3.04±0.81)mg/L vs.(6.32±1.45)mg/L] in the research group were lower than those in the control group, the differences were statistically significant(t=22.329, 9.798, 9.304, 16.523, all P<0.05). After treatment, the systolic blood pressure[(131.25±5.74)mmHg vs.(139.61±5.59)mmHg] and diastolic blood pressure[(85.23±5.07)mmHg vs.(90.43±5.81)mmHg] in the research group were lower than those in the control group(t=8.730, 5.642, all P<0.05). The cesarean section rate(61.43% vs.87.14%) and intrauterine distress rate(1.43% vs.11.43%) in the research group were lower than those in the control group(χ2=12.115, 5.818, all P<0.05). Conclusion Compound salvia miltiorrhiza injection has significant control effect on oxidative stress and inflammatory reaction in patients with gestational hypertension, and is beneficial to reduce the complications of childbirth. Key words: Hypertension, pregnancy-induced; Oxidative stress; Inflammation; Pregnancy complications; Parturition; Blood pressure; Pregnancy outcome; Compound salvia injection

Effects of the combined therapy of heat sensitive moxibustion and acupoint injection on endometrial receptivity of hypdrosalphinx infertility in the patients after hysteroscopy and laparoscopy

To explore the effects of the combined therapy of heat sensitive moxibustion and acupoint injection on endometrial receptivity of hypdrosalphinx infertility in the patients after hysteroscopy and laparoscopy on the base of routine post-operative anti-inflammation. A total of 210 patients of hypdrosalphinx infertility after hysteroscopy and laparoscopy were divided into a combined therapy group, a heat sensitive moxibustion group and a control group, 70 cases in each one according to the random number table. In the control group, the intravenous drip of cefoxitin sodium was adopted, and the anti-inflammation treatment was given for 1 week after operation. In the heat sensitive moxibustion group, on the basis of the treatment as the control group, the heat sensitive moxibustion was applied after vaginal bleeding stopped. The acupoints were Yaoyangguan (GV 3), Guanyuan (CV 4), Qihai (CV 6), Shenshu (BL 23), Sanyinjiao (SP 6), Yinlingquan (SP 9) and Zigong (EX-CA1). The acupoints were modified according to the different syndromes. In the combined therapy group, on the basis of the regimen as the heat sensitive moxibustion group, after vaginal bleeding stopped, the acupoint injection was given alternatively at bilateral Tiangong (extra, 1.0 cm inferior and bilateral to the cervix) with lidocaine 1 mL, amikacin 2 mL and salvia injection 2 mL. The treatment was given once every day, the treatment for 10 times as one course and a total of 3 courses were required. The endometrial type, thickness, uterine arterial plusatility index (PI) and resistance index (RI) were observed in the patients of each group. After treatment, the numbers of A-type endometrial type in the combined therapy group and the heat sensitive moxibustion group were remarkably higher than those of the control group [57.1% (40/7) vs 31.4% (22/70), 50.0% (35/70) vs 31.4% (22/70), both P<0.05]. The endometrial thickness after treatment was all increased as compared with that before treatment in each group (all P<0.05). The increasing degree in the combined therapy group was better than either the heat sensitive moxibustion group or the control group (both P<0.05). The improvement in the heat sensitive moxibustion group was superior to the control group (P<0.05). PI and RI in the combination group and PI in the control group were decreased after treatment (all P<0.05). The improvements of PI and RI in the combination group were better than those in the heat moxibustion group (both P<0.05), which were superior to those in the control group (all P<0.05). In the patients of hypdrosalphinx infertility after hysteroscopy and laparoscopy, the combined therapy of heat sensitive moxibustion and acupoint injection increases endometrial thickness, reduces uterine arterial resistance and improves endometrial receptivity.

Effects of Salvia injection on synaptic plasticity of neonatal rats with hypoxic-ischemic brain damage

Objective To observe the effects of Salvia injection on the brain pathology, expression of synaptophysin and the synaptic ultrastructure at different time points in neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods One hundred and fifteen healthy newborn SD rats of 7-day-old were randomly divided into normal group, sham group, Salvia high dose group, Salvia low dose group, and the HIBD model group.The HIBD model was prepared by ligation of the left carotid artery combined with hypoxic environment.The rats(in normal group, sham and HIBD model groups) were injected with sterile saline[9.0 mL/(kg·d)], while the rats in high and low dose groups were injected with Salvia injection [9.0 mL/(kg·d) and 4.5 mL/(kg·d) respectively]. The intraperitoneal injection lasted for 7 and 14 days.The rats' brains were collected at one day, 7 days and 14 days after the modeling respectively.The specimens of brain tissue were observed through hematoxylin-eosin (HE), the expression of synaptophysin(SYP)was determined by using immunohistochemistry method, and the synaptic ultrastructure in the frontal cortex was observed through transmission electron microscope. Results Finally, 106 newborn rats were included in statistics analysial.(1)General observation: after HIBD modeling, the neonatal rats were mostly in the left-lateral position, with difficulties in turning over, balance abnormalities, limb shaking and other abnormal behaviors, and the delay of eyes open with the left upper eyelid ptosis.(2)Pathology: the disordered and deep stained nerve cells, the degenerated and necrotic neurons were observed in the brain tissues of the HIBD model group.(3)Expression of synaptophysin: mean density of SYP in the HIBD model group was significantly lower than the normal group on day 1, day 7, and day 14 (P<0.05); the SYP expression in the Salvia intervention groups increased compared with the HIBD model group(P<0.05). (4)Synaptic ultrastructure: the incomplete structure of the frontal cortex neurons, the swelling organelle and the synaptic structure damage were observed in the HIBD rats.Compared with the HIBD model group, the neuronic and synaptic ultrastructure were improved by the intervention treatment of high and low dose Salvia injection. Conclusions The mechanism of Salvia injection in treatment of neonatal rats with HIBD may be associated with the improvement in neuronal ultrastructure and synaptic reorganization. Key words: Salvia injection; Hypoxic-ischemic brain damage; Synaptic plasticity; Ultrastructure; Synaptophysin

A Long-Term Study of Efficacy of Patients with Macular Edema Secondary to BRVO Treated with Ranibizumab Combined with Compound Salvia

Purpose: To study the long-term efficacy of intravitreal ranibizumab injection combined with in-travenous compound salvia injection drip in the treatment of patients with macular edema sec-ondary to branch retinal vein occlusion(BRVO). Methods: Sixty-five patients of branch retinal vein occlusion with macular edema were analyzed retrospectively. Thirty-seven patients in the treat-ment group were treated with ranibizumab injection combined with intravenous compound salvia injection drip, twenty-eight patients in the control group were treated with ranibizumab injection only. All patients were recorded and analyzed changes of clinical efficacy after 3 months, 6 months after a course of treatment of 6 months. Results: During 3 months after 6 months’ treatment, there were 5 patients having recurrence in the treatment group, while there were 9 in control group, the rate of recurrence between the two groups had significant difference (P < 0.05); during 3 - 6 months after a course of treatment, there were 2 patients having recurrence in the treatment group, while there were 4 in control group, the rate of recurrence between the two groups had significant difference (P < 0.05); however, the treatment group’s BCVA was better than control group, and the difference between the two groups had significance (P < 0.05). Conclusion: Ranibizumab injection combined with intravenous compound salvia injection drip could effectively reduce the impossibility of recurrence, improve the visual activity and bring better therapeutic efficacy in patients with macular edema secondary to branch retinal vein occlusion. The treatment showed great potential in the clinical use.

Clinical curative effect observation on Kudiezi injection treatment of anterior ischemic optic neuropathy

Objective To study the clinical curative effects of Kudiezi injection treatment of anterior ischemic optic neuropathy.Methods In our hospital from January 2009 to September 2010,53 patients (53 eyes) with anterior ischemic optic neuropathy were treated and randomly divided into two groups,group Ⅰ was treated with Kudiezi injection treatment; group Ⅱ was treated with Salvia injection fluid therapy,two groups were compared on two weeks of treatment efficacy.Results Among 27 patients in group Ⅰ,cured in 6 cases,8 cases excellent,8 cases effective,total effective rate was 81.48％; while in 26 patients of group Ⅱ,0 case cured,excellent in 4 cases,effective in 6 cases,total effective rate was 38.46％.The curative effect of group Ⅰ was significantly better than that of group Ⅱ,P ＜ 0.05.Conclusion Kudiezi injection treatment to anterior ischemic optic neuropathy can achieve better efficacy results,visual acuity in patients with good vision to expand,which is worthy to be widely used in clinic. Key words: Anterior ischemic optic neuropathy; Kudiezi injection; Clinical curative effect

The injection on myocardial protection and prognosis of acute organophosphorus pesticide with Salvia miltiorrhiza

Objective To investigate the effects of hemodynamic,prognostic effect and myocardial protection of Salvia injection on patients with acute organophosphorus pesticide poisoning. Methods 68 cases of patients with acute organophosphate were divided into observation group（n=34）and control group（n=34） from January 2012 to December 2013.The control group were given with atropine detoxification （dose：5-10mg）, Tiopronin （0.2 g/d, 1 times/d） liver treatment, Pioneer will（2 g/second,1/d）.The observed group received the foundation treatment and Salvia injection（30ml, 1/d）, 7d course of treatment. The myocardial enzymes and hemodynamic parameters of two groups were observed before and after treatment. Results The survival rate and died rate of observation group were 94.12%（32 cases ）, 5.88%（2 cases ）. The survival rate and died rate of control group were 79.41%（27cases ）and 20.59%（7cases）. The survival rate and died rate of two groups were significant difference （χ2=5.123, P〈0.05）. The myocardial enzymes （AST, CK, CK-MB, LDH, HBDH） of observation groupafter 2d each index[were（58.6±22.7）U/L, （412.6±156.9）U/L, （78.6±＆amp;nbsp;35.2）U/L, （489.3 ± 112.3）U/L, （412.8 ± 259.6）U/L] and blood rheology （ESR, FIB, LBV, HBV）each index[were（14.36±4.19） mm/h before , （259.3±23.14）g/L, （7.17±1.12）mPa？s, （4.12±0.81）mPa？s]were lower than in the same group therapy [myocardial enzymes indexes were （131.3±32.5）U/L, （1324.5± 345.2）U/L, （187.5 ± 72.2）U/L, （914.5 ± 312.2）U/L, （812.3 ± 312.2）U/L; hemorheology indexes were （23.29±3.49）mm/h, （389.57±34.24） g/L, （10.4±1.3）mPa？s, （6.3±1.2）mPa？s]. 5 d after treatment control group myocardial enzymes（AST, CK, CK-MB, LDH, HBDH）each index[were（85.3±22.8）U/L, （486.3± 78.9）U/L, （67.8±11.2）U/L, （542.3±78.6）U/L, （225.9±112.4）U/L]and hemorheology indexes[were（17.7± 4.6）mm/h, （289.4±32.5）g/L, （8.9±1.2）mPa？s, （5.6±1.3）mPa？s] was significantly lower than in the same group before treatment[myocardial enzymes indexes were（128.3±29.3）U/L, （1298.6±329.4）U/L, （182.6± 70.6）U/L, （902.3±286.3）U/L, （803.6±293.6）U/L;hemorheology indexes were （23.9±3.5）mm/h, （382.6± 32.5）g/L, （10.3±1.1）mPa？s, （6.2±1.1）mPa？s, P〈0.05]. Conclusion Salvia injection can effectively improve the hemodynamic indicators of acute organophosphorus pesticide poisoning patients , reduce myocardial damage, promote patient prognosis. Key words: Salvia injection; Acute organophosphorus; Myocardium; Hemodynamic

Protective effect of Zinc and Salvia on the immune system caused by noise in rats

Objective To observe the protective effect of the zinc gluconate combined with Salvia injection on noise induced serum immunoglobulin(IgG,IgM,IgA) and immune molecules (IL-2,IL-4) in rats.Methods 50 female SD rats were randomly divided into control group,noise group,zinc gluconate noise group(plus zinc group),Salvia injection noise group (plus Salvia group) and Zinc gluconate oral liquid and Salvia injection noise group(combined group),10 rats in each group.Except the control group,rats in the other groups were continuously exposed to high frequency steady noise for two weeks.The concentrations of serum immunoglobulin (IgG,IgM,IgA) and immune molecules (IL-2,IL-4) after the intervention of noise were compared.Results Serum levels of IgG,IgM,IL-2,IL-4of the control group were significantly higher than the noise group (all P ＜ 0.05),plus zinc group (all P ＜ 0.05),plus Salvia group(all P ＜0.05),the differences were statistically significant.Serum level of IgA of each group was not statistically different compared with control group (all P ＞ 0.05).Serum levels of IgG,IgM,IL-2,IL-4 in the noise group were significantly lower than those in the control group (all P ＜ 0.05),combined group (all P ＜ 0.05),the differences were statistically significant.Serum levels of IgG,IgM,IL-2 in plus zinc group were significantly higher than the noise group(all P ＜ 0.05).Serum IgA concentration of each group showed no significant difference compared with noise group(all P ＞ 0.05).Conclusion Zinc gluconate oral solution and Salvia injection have protective effect on immune system affected by noise in rats,the protective effect of combination of zinc gluconate oral solution and Salvia injection is stronger than the individual. Key words: Noise; Zinc gluconate; Salvia injection; Immunoglobulin ; Immune molecules

Effect of Zinc and Salvia on some whole blood metal ion distribution caused by noise in rats

Objective To observe the protective effect of the separate zinc gluconate oral and combined of Salvia injection on whole blood metal ion of calcium,magnesium,iron,copper and zinc concentration in noise-induced rats.Methods 50 female SD rats were randomly divided into a control group,a noise group,a zinc gluconate noise group (plus zinc group),a Salvia injection noise group (plus Salvia group) and a Zinc gluconate oral liquid and Salvia injection noise group (combined group),with10 rats in each group.Except the control group did not expose to noise,the rest groups were continuously exposed to high frequency steady noise for two weeks.Each group was compared for the concentration differences of whole blood metal ion of calcium,magnesium,iron,copper and zinc after the intervention of noise.Results ① In each group at the comparison of the calcium ion concentration: Calcium ion concentration of the control group(1.25± 0.16)mmol/L and the combination group(1.27 ± 0.10) mmol/L was significantly lower than the noise group (1.42 ± 0.18) mmol/L.The rest groups compared to each other were not statistically significant.②Magnesium ion concentration was highest in the noise group (1.53 ± 0.10)rmtmol/L),and lowest in the control group (130 ± 0.29) mmol/L,and the noise group was significantly higher than that of the control group.The mean of magnesium ion concentration in plus zinc group (1.42± 0.27) mmol/L,plus Salvia group (1.38± 0.15) mmol/L and combined group(l.37±0.11)mmol/L were lower than the noise group,but the difference was not significant (P＞0.05).③ The iron ion concentration of the noise group (5.47± 1.29)mmol/L was significantly lower than the other four groups (P＜0.05).The control group,plus zinc group,plus Salvia group,the combined group showed no significant differences.④ Whole blood copper ion concentration of the noise group (16.69 ± 4.18) μmol/L was significantly lower than the control group (21.53 ± 3.78) μmol/L and the combination group(19.53± 1.92)μmol/L with a statistical difference; compared with the control group,the concentration of copper ions in plus zinc group(16.19± 1.93)mμol/L was significantly lower (P＜0.05).⑤The whole blood zinc ion concentration in the noise group (50.83±7.99)μmol/L was significantly lower than the other groups,zinc ion concentration in the plus Salvia group (53.87±6.77)μmol/L was significantly lower than the control group (63.86± 8.83) μmol/L; the whole blood zinc ion concentration showed no difference between the plus zinc group (54.81 ± 5.90) μmol/L,plus Salvia group and combined group (59.21 ± 3.90) μmol/L.Conclusion Combined zinc gluconate oral solution and Salvia injection had protective effect on whole blood metal ion concentration affected by noise.The protection effect of zinc gluconate oral solution and Salvia injection combination was stronger than any individual. Key words: Zinc gluconate oral solution; Salvia Injection; Noise; Metal ion

The influence of fasudil on the serum hs-CRP and SAA in the treatments of patients with acute cerebral infarction

Objective To study the effectiveness of fasudil on the serum high sensitive C-reactive protein (hs-CRP) and serum amyloid A (SAA) in the patients with acute cerebral infarction.Methods 100 patients with acute cerebral infarction were randomly divided into two groups,the control group (n =50 cases) and the treatment group(n =50 cases).The patients in the control group were treated through salvia injection,while the patients in the treatment group were treated through fasudil.They were treated for forteen days.The hs-CRP and SAA in the serum were detected before and after treatment.Results The total efficienties were 92％ in the treatment group and 84％ in the control group.There was a significant difference between two groups(x2 =1.5152,P ＜ 0.05).The treatment group before and after treament with hs-CRP were (11.3 ± 3.7) mg/L,(3.6 ± 1.1) mg/L.The control group before and after treatment with hs-CRP were (10.9 ± 3.5) mg/L,(7.2 ± 2.8) mg/L.Before and after treatment two groups had significant difference (t =14.1053,P ＜ 0.01) ; after the treatment in the two groups had significant diffecence (t =8.4618,P ＜ 0.05).The treatment group before and after treatment with SAA were (17.6 ± 5.2) mg/L,(2.7 ± 0.9)mg/L,control group before and after SAA treatment were (17.1 ± 4.9) mg/L,(5.2 ± 1.8) mg/L.Before and after treatment,two groups had significant difference (t =2.0096,2.0096,all P ＜ 0.01) ; after the treatment in the two groups had significant difference(t =8.7841,P ＜ 0.05).Conclusion Fasudil can improve acute cerebral infarction throughmitigating inflammation. Key words: Brain infarction; C-Reactive protein; Amyloid protein A; Fasudil

Effects of Ginkgo Bilobate Extract Injection on the hemorheology, blood lipid and C-reactive protein in serum of patients with cerebral infarction

Objective To observe effects of ginkgo bilobate extract injection on the hemorheology,blood lipid and c-reactive protein in serum of patients with cerebral infarction. Methods 62 cases patients with cerebral infarction were randomly divided into two groups by means of random number table. Two groups were both given routine treatment. The control group was treated with 20ml compound salvia injection, which was added to 250 ml normal saline, once a day, continuous therapy for 14 days, and the treatment group was treated with 20ml ginkgo bilobate extract injection, which was added to 250 ml normal saline, once a day, continuous therapy for 14 days. The clinical symptoms were observed, hemorheology related index, level of blood lipid and c-reactive protein were detected before and after the treatment. Results Compared with the control group,hemorheology related index decreased significantly in the treatment group, especially whole blood viscosity ( 1.57±0.28) mpa ·s (P＜0.01). Blood reduced viscosity (13.95 ± 2.07) mpa ·s, erythrocyte aggregation index (1.37±0.12) , hematocrit (42.36±8.16) were also decreased significantly (P＜0.05) .Triglyceride (TG)(1.23±0.12) mmol/L、 cholesterol (TC) (4.29±1.17) mmtmol/L、 low density lipoprotein cholesterol (LDIL-C)(2.31± 0.39) mmol/L and c-reactive protein ( 10.48 ±- 3.82) mg/L in serum in the treatment group were lower than those of the control group (P＜0.01 orP＜0.05) , high density lipoprotein cholesterol (HDL-C) ( 1.78±0.76) mmol/L in serum was higher than that of the control group (P＜0.01) .The total effective rate of the treatment group and the control group was 87.1％ and 67.7％ respectively, there was a significant difference (x2=3.553, P＜0.05). Conclusion The ginkgo bilobate extract injection can protect cerebral infarction from injury,which may be related with its action on regulating the blood viscosity and level of blood lipid. Key words: Cerebral infaruction; Ginkgo bilobate extract injection; Edaravone injection

Investigation on the methods to prevent radiotherapy-induced oral mucositis

Objective To investigate effective methods for preventing radiotherapy - induced oral mucositis among patients with cephalocervical malignant tumors. Methods 92 patients with cephalocer-vical malignant tumors treated with radiotherapy were randomly divided into group A (31 cases),group B (31 cases) and group C (30 cases). Mixture of 20 ml heartleaf houttuynia herb injection,20 ml salvia injection and 30 ml distilled water were given to patients in group A by means of nebulization inhalation one hour before and after every radiotherapy treatment, the inhalation lasted 20 minutes each time up to the end of radiotherapy. Mixture of 2 ml Kang-fu-xin injection,0.5 mg vitamin B|2 injection and SO ml normal saline were given to patients in group B by the same means as group A. Yangyin Qingfei decoction were given to patients in group C by means of selecting its 500 ml juice for oral use. Half of the 500 ml decoction were separately given 1 hour before and after each radiotherapy treatment up to the end of radiotherapy. Results The incidence rates of oral mucositis were obviously lower among patients in group A than in group B and C. Conclusions Nebulization inhalation of mixture of heartleaf houttuynia herb and salvia injections has significant clinical effects on oral mucositis after radiotherapy. Key words: Houttuynia cordata; Salvia injection; Radiotherapy; Oral mucositis

Effect of composite salvia injection on platelet parameters in children with anaphylactoid purpura

To explore the effect of composite salvia injection (CSI) on platelet parameters in children with anaphylactoid purpura (AP) and its clinical significance. One hundred and fifty children with AP were assigned to two groups, 80 in Group A and 70 in Group B. They were treated, respectively, with conventional therapy only or conventional therapy combined with CSI. Their platelet parameters, including blood platelet count (BPC), mean platelet volume (MPV), platelet distribution width (PDW) and plateletcrit (PCT) were determined at the acute stage and convalescent stage, respectively. At the acute stage, the BPC in AP children of both groups was in the normal range, but significant abnormality was shown in the levels of MPV, PDW and PCT. As for comparisons of these parameters at the convalescent stage, significant difference between the two groups was also shown in terms of MPV, PDW and PCT (P<0.05 or P<0.01). Although platelet shows no quantitative change in the pathogenic process of AP, important functional changes are surely existent. CSI could promote the normalization of platelet function.

Observation on Curative Effect of Treating Acute Myocardial Infarction with Salvia Injection and Astragalus Injection

Objective To observe clinical effect of treating acute myocardial infarction with western routine treatment combined with salvia injection and astragalus injection.Methods 100 cases patients with acute myocardial infaretion were randomly divided into a treatment group and a control group.The treatment group was treated with western routine treatment combined with salvia injection and astragalus injection,and the control group was treated with western routine treatment,which Were injected by vein for one time daily.Results The therapeutic effects in the treatment group were significantly better than the control group,with P＜0.05.Conclusion Salvia injecfion and astragalus injection has better effects on improving parameters of hemorheology and anti-free radical,enhancing cfinical effect of acute myocardial infarction,incrrasing cardiac function after acute myocardial infarction. Key words: Acute myocardial infarction; Salvia injection; Astragalus injection

Observation and nursing of Salvia and Astragalus injection coronary heart disease and angina pectoris curing

目的 探讨丹参黄芪注射液治疗冠心病心绞痛的临床效果.方法 将80例冠心病心绞痛患者随机分为两组,每组各40例.治疗组采用静脉滴注丹参、黄芪注射液,对照组采用静脉滴注丹参注射液,连续观察2个疗程,每个疗程14 d,并对两组疗效进行对比.结果 发现治疗组治疗效果优于对照组(P＜0.05).结论 应用丹参、黄芪联合治疗能缓解心绞痛症状、改善心电图及血液流变学指标,改善心肌缺血,保护受损心肌,改善血液循环,增强心功能等作用.治疗期间同时实施系统、有序的观察和护理措施,可取得满意的治疗效果。

Clinical research of acute stroke treatment using acupuncture

Objective: To study the clinical effects of acupuncture therapy for acute stroke. Methods: 150 patients suffered from acute stroke were randomly divided into two groups: drug group, acupuncture group. To apply routine drug treatment of Neurology for drug group: pisacetam 250mL, Salvia injection 20mL added into normal sodium 250mL for intravenous drip, once a day. For acupuncture group applied "calm the liver and suppress yang" acupuncture therapy beside drugs. Modified Scandinavian Stroke Scale (SSS) and activities of daily living (ADL) scale (Barthel Index) were used for evaluation, as well as biochemistry\hemorheology determination. Results: The scale showed significant difference as compared with the drug group, biochemistry index had non-significant difference, and hemorheological index had partly improved. Conclusion: The scale could synchronously reflect the clinical symptom and changes of patient with stroke.Acupuncture therapy has a significant improvement of acute stroke treatment.

Effect of ritodrine hydrochloride combined with radix salviae miltiorrhizae on fetal growth restriction

Objective Ritodrine hydrochloride(RH) combined with radix salviae mihiorrhisa(RSM) is ap- plied to treat fetal growth ristriction(FGR). Curative effect was recorded during observation. Methods 62 pregnant women with slowly growing fetuses were divided into two groups randomly. Group A(test group) :including 31 preg- nant women who received RH combined with RSM treatment. Group B(control group) :including the other 31 preg- nant women who only received RSM treatment. Group B received 250ml of fat emulsion injection. One time every three days within one week. At the same time,gronp B also received injection of 500ml 10 % dextrose, coenzyme A 100U ,ATP 40mg,vitamin C 2g,dextran-40 and compound salvia injection 10ml one time a day,ten days in all. Be- sides the above-mentioned medicament for group B, group A received RH 100ml injection every day. Ten days was a period of treatment. After the treatment, uterine height, abdominal circumference, weight were measured and bipari- etal diameter, head circumference, abdominal circumference were checked by B-type ultrasonic inspection. After birth,weight of the baby and weight of placenta were measured too. Results Measurement indexes from group A, weight of the new-born baby and weight of placenta are obviously higher than those from group B. Conclusion RH combined with RSM can obviously extend blood vessel, loose placenta, improve blood circulation, accelerate cell metabolism, make microeirculation better, reduce permeability of capillary vessel, keep placenta's function and help foetus' growth. Key words: Fetal growth retardation; Ritodrine; Salviae miltiorrhiza; Drug therapy, combination

The morphologic characteristic of neuron-like cells of human umbilical cord derived mesenchymal stem cells

目的 研究人脐带华尔通胶来源的间充质干细胞(MSCs)分化为神经细胞的形态学特征,为神经细胞移植探索细胞来源.方法 用复方丹参注射液诱导人脐带来源的MSCs向神经细胞分化,用光学和电子显微镜对分化和未分化的细胞进行观察;了解细胞分化同形态学改变的关系.结果 丹参可诱导人脐带MSCs向神经样细胞分化,光镜下细胞变短,体积变小,细胞形成双极或多极的细胞体,细胞呈星型,部分细胞的胞质收缩形成细胞的突起;扫描电镜下观察,复方丹参注射液诱导后,细胞胞体形成多样的神经样轴突,细胞之间形成网络样的连接;透射电镜下,丹参诱导后,细胞内结构、细胞器发生明显变化,细胞成熟,胞质内可见大量的线粒体,粗面内质网和游离的核糖体等,诱导后的细胞可见尼氏体样结构--大量排列的粗面内质网和广泛分布的游离核糖体和发达的线粒体;诱导后的细胞也表达神经细胞的标记.结论 复方丹参注射液不仅诱导人脐带来源的MSCs发生神经样细胞的形态学改变,同时伴发有细胞分化的过程和神经细胞标记表达,证明人脐带来源的MSCs具有分化为神经细胞的能力。

Induction of Human Umbilical Cord Blood Mesenchymal Stem Cells into Nerve-Like Cells by Salvia Miltiorrhiza.

Salvia miltiorrhiza is a traditional Chinese herb with thousands of years history. Composite salvia injection (CSI), a widely used administration in China nowadays, consists of Salvia miltiorrhiza, notoginseng, radix and borneo. It can promote blood flow, but also regulate qi to alleviate pain. So it is frequently used for angina, coronary heart disease and chest discomfort. Recently, some laboratory studies show that CSI may have the effect of cell induction.. In this study, we aim to investigate its neural induction.Objective: To investigate the differentiation-inducing effects of salvia miltiorrhiza on umbilical cord blood mesenchymal stem cells.Methods: The immunophenotypes of human umbilical cord blood-derived mesenchymal stem cells(MSCs) were determined by flow-cytometry. Salvia miltiorrhiza was adapted to induce the differentiation of the primary cultured cells of umbilical cord blood and MSCs. The results were compared with nerve growth factor(NGF) and ganglioside(GM1). The differentiated and undifferentiated cells were identified with immunocytochemistry.Results: In primary culture, certain cells proliferated and formed cell clusters. These cells were either fibroblast-like or round in shape with various sizes. The positive rates for MSCs phenotypes CD29, CD44, CD59 of primary culture cells were 10.7%,37.27% and 66.67%, respectively. Salvia miltiorrhiza could induce the primary cultured cells of umbilical cord blood to express neural phenotypes. After passage, the MSCs could be maintained in vitro for extended periods with stable population doubling. The positive rates for CD29, CD44, CD59 of human umbilical cord blood-derived MSCs were 40.2%,70.5% and 95.4%, respectively. Salvia miltiorrhiza could induce the MSCs to express three kinds of proteins: nestin(the marker of neuronal precursor stem cells), β-TubulinIII and neurofilament (NF) (the marker of neuronal phenotypes) and glial fibrillary acidic protein(GFAP, the marker of astrocyte). Besides, NGF and GM1 could also induce the same MSCs to nerve-like cell, which could express the same markers: β-TubulinIII, NF and GFAP. In comparison NGF and GM1, the induction effect of salvia miltiorrhiza demonstrated the higher rate of expressing neuron phenotypes at a faster speed.Conclusion: The human MSCs could be isolated from human umbilical cord blood. Salvia miltiorrhiza can induce the MSCs to differentiate into nerve-like cells, therefore, might act as a potential drug for neural induction.

A clinical study of Safflower Yellow injection in treating coronary heart disease angina pectoris with Xin-blood stagnation syndrome

To evaluate the clinical effect and safety of Safflower Yellow injection (SYI) in treating coronary heart disease angina pectoris (CHD-AP) with Xin-blood stagnation syndrome (XBSS). Adopted was the multi-centered, randomized, positive parallel controlled method, 448 patients with CHD-AP-XBSS were enrolled and divided into two groups, 336 in the tested group treated with SYI and 112 in the control group treated with Salvia injection by intravenous dripping once a day for 14 days, so as to observe the conditions of angina, electrocardiogram, and therapeutic effect on traditional Chinese medicine (TCM) symptoms as well as the safety of the treatment. The significantly effective rate and total effective rate in the tested group were 60.06% (194/323) and 91.02% (294/323) respectively; Those in improvement of TCM symptoms were 40.18% (129/321) and 75.23% (243/323) respectively, which were better than those in the control group (P < 0.01). SYI Injection is effective and safe in treating CHD-AP-XBSS.

Effect of the principle of activating blood circulation to break stasis on GMP-140 and D2 dimer in patients with acute cerebral infarction

To explore the clinical efficacy of the principle of activating blood circulation to break stasis (ABCBS) and its influence on platelet membranous protein particle (GMP-140) and D(2) dimer (D-dimer) before and after treatment. Eighty-eight patients with blood stasis syndrome (BSS) of acute cerebral infarction (ACI) were randomly divided into two groups, both of which were treated with conventional treatment, i.e. with western medicine (WM), with Salvia injection added through intravenously dripping. One of the two groups was used as the control and the other group as the treated group who had ABCBS herbs orally taken in addition. The duration of treatment course for both groups was 3 weeks. There were changes in both groups over clinical symptoms, nerve function deficit scoring and GMP-140, D-dimer, but the treated group showed significantly better than that of the control group, (P < 0.05). ABCBS principle could serve as an important auxiliary treating method for BSS of ACI, as it can effectively alter the blood of ACI patients which was viscous, condense, coagulant and aggregating.