Rutin Equivalents Research Articles

Green extraction of bioactive compounds from Thuja orientalis leaves using microwave- and ultrasound-assisted extraction and optimization by response surface methodology

Thuja orientalis is a medicinal plant. To extract its bioactives on a large scale, an efficient and eco-friendly method is required. In this study, two techniques, microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), were optimized using response surface methodology (RSM) with 70% hydro-ethanol as an extractant. The responses included total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity measured through DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (RSA) assays, and metal chelating activity (MCA). Well-fitted models were obtained for both techniques. In MAE, the optimized conditions were solvent-to-feed ratio (SFR) 35 mL/g, power 640 W, and time 90 s with optimum TPC of 540.4 mg gallic acid equivalent (GAE)/g dry weight (DW), TFC of 63.03 mg rutin equivalent (RE)/g DW, DPPH-RSA of 69.17%, ABTS-RSA of 77.69%, and MCA of 53.46%. In UAE, the optimum conditions were amplitude 90%, time 2 min, and SFR 35 mL/g and the optimum response values were TPC of 190.5 mg GAE/g DW, TFC of 48.59 mg RE/g DW, DPPH-RSA of 81.73%, ABTS-RSA of 73.63% and MCA of 63.81%. Scanning electron microscopy showed that both MAE and UAE effectively fractured the cell matrix. Both techniques were found highly effective, MAE being more efficient in TPC, TFC, and ABTS-RSA, while UAE showed higher efficiency in DPPH-RSA and MCA. Overall, MAE can be used on an industrial scale for the extraction of polyphenols, antioxidants, and metal chelators from Thuja orientalis being a less time- and solvent-consuming green technique.

Investigation of The Antioxidant Activity of Cinnamon Bark Extracted with Different Solvents

The objective of this study was to evaluate the effect of methanol, ethyl acetate, and hexane for the extraction of cinnamon bark (Cinnamomum burmanni) (CB) on the contents of the total phenolic, total flavonoid and its 1,1,-diphonyl-1-picryl-hydrazyl (DPPH) radical scavenging activity. CB samples were ground with grinder machine in one mm sieve and extracted with treatments to determine parameter measured. All CB samples were extracted using different treatments. Parameter measured were total phenolic, total flavonoid and DPPH. CB was ground and extracted by using three treatments, data collected was statistical analysis and followed with Duncant Test, The highest total phenolic content found when CB extracted with methanol with the value of 111.43 mg gallic acids equivalent (GAE)/g DW while the lowest amount detected in hexane with the amount of 57.23 mg GAE/g dry weight (DW). It was significantly different (P<0.05) from those extracted with ethyl acetate and hexane. There was significant (P<0.05) different for the content of flavonoid among all treatment groups. Methanol was the highest solvent and hexane was the lowest with the amount of 286.27 and 64.73 mg rutin equivalent (RE)/g DW respectively. There was significantly different (P<0.05) for scavenging activity among all treatment. Using methanol in extracting CB showed the highest scavenging activity of 69.59 % among all other treatment groups. It could be concluded that Methanol was the best solution in extracting CB

Chemical Characterization of Different Extracts from Artemisia annua and Their Antioxidant, Enzyme Inhibitory and Anti-Inflammatory Properties.

Artemisia annua L. (Asteraceae Family) is an important plant in Asia that has been used for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation. In this study we aimed to evaluate the effects of different polarity extracts (hexane, dichloromethane, ethyl acetate, ethanol, ethanol/water (70 %) and water) from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS. In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated. The water extract contained the highest content of the total phenolic with 34.59 mg gallic acid equivalent (GAE)/g extract, while the hexane had the highest content of the total flavonoid (20.06 mg rutin equivalent (RE)/g extract). In antioxidant assays, the polar extracts (ethanol, ethanol/water and water) exhibited stronger radical scavenging and reducing power abilities when compared to non-polar extracts. The hexane extract showed the best AChE, tyrosinase and glucosidase inhibitory effects. All extracts revealed effective anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression. These effects seemed to be not related to the only phenolic content. However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.

Assessment of the Phytochemical Profile, Antioxidant Capacity, and Hepatoprotective Effect of Andrographis paniculata against CCl4-Induced Liver Dysfunction in Wistar Albino Rats.

Recent studies have highlighted the necessity to thoroughly evaluate medicinal plants due to their therapeutic potential. The current study delves into the phytochemical profile, antioxidant capacity, and hepatoprotective effect of Andrographis paniculata. The investigation specifically targets its effectiveness in mitigating liver dysfunction induced by carbon tetrachloride (CCl4) in Wistar albino rats, aiming to uncover its promising role as a natural remedy for liver-related ailments. A. paniculata leaf extract was screened for phytoconstituents and antioxidant and hepatoprotective effects in Wistar albino rats against CCl4-induced liver dysfunction. Phytochemical analysis revealed the presence of flavonoids, alkaloids, and phenolic compounds in all extracts. The phenolic concentration ranged from 10.23 to 19.52 mg gallic acid per gram of the sample, while the highest flavonoid concentration was found in the ethanol fraction (8.27 mg rutin equivalents per gram). The antioxidant activity varied from 10.23 to 62.23. GC-MS analysis identified several phytochemicals including octadecanoic acid, stigmasterol, phenanthrenecarboxylic acid, and others. Effects of the ethanol extract of A. paniculata were evaluated in four groups of animals. Biochemical estimations of serum glutamine oxaloacetate transaminase, serum glutamine pyruvate transaminase, and serum bilirubin were significantly higher (p < 0.05) in the CCl4-treated group. Treatment with 300 mg/kg b.w. of the ethanol extract of A. paniculata significantly (p < 0.05) decreased these serum enzymes. Lipid peroxidation levels in carbon tetrachloride-treated animals showed a substantial (p < 0.05) rise when compared to untreated animals, while the lipid peroxidation levels were considerably (p < 0.05) reduced after treatment with ethanol extract at 300 mg/kg b.w. Liver biochemical catalase activities were significantly reduced in the carbon tetrachloride-treated animals. The results of this study conclusively demonstrate that A. paniculata extracts are a rich source of phytochemicals and possess significant antioxidant, free radical scavenging, and hepatoprotective properties.

Lycium barbarum ethanol extraction: Application as cosmetic product ingredients

Plant seed oils and extracts are widely used in cosmetic industry to provide the skin with a variety of benefits. Due to the paucity of bioactivity studies on Lycium barbarum and its skin care applications, Lycium barbarum seed oil (LBSO) was utilized to create stable oil-in-water emulsions, and Lycium barbarum fruit extract (LBE) was used as a functional ingredient in cosmetic formulations We optimized the biological activity, storage stability, and physicochemical properties of LBSO when added to O/W emulsions (antioxidant, anti-tyrosinase, and anti-aging). The LBE emulsion [15 % L. barbarum seed oil (F3) and 1 % LBE] showed the highest physical and storage stability (pH 6.02). TPC and TFC had respective values of 2.146 ± 0.013 mg Gallic acid equivalent (GAE)/g and 1.099 ± 0.012 mg Rutin equivalent (RE)/g. For anti-aging purposes, LBE emulsion could inhibit hyaluronidase (44.74 ± 2.05 %), elastase protease (26.59 ± 2.56 %), and melanogenesis (34.35 ± 1.77 %). Overall, our current findings speculate the development of anti-aging cosmetic products using L. barbarum.

Chemical characterization and biological abilities of Anthocleista djalonensis collected from two locations of Ivory Coast

AbstractIn this study, the total phenolic and flavonoid contents, HPLC‐DAD detected phytochemicals, antioxidant and enzyme inhibitory potential of methanolic and aqueous (as infusion) extracts of the medicinal plant, Anthocleista djalonensis (leaf and stem bark) collected from two locations, Mafiblé and Prikro, in Ivory Coast, were investigated. The ranges of total phenolic and flavonoid contents obtained were 14.17–46.95 mg gallic acid equivalent (GAE)/g and 2.96–34.76 mg rutin equivalent (RE)/g, respectively. Antioxidant abilities in terms of radical scavenging, reducing and metal chelating activity of the extracts in different assays were as follows: DPPH (4.90–48.82 mg trolox equivalent [TE]/g), ABTS (21.05–81.89 mg TE/g), CUPRAC (29.54–122.33 mg TE/g), FRAP (17.53–94.06 mg TE/g) and metal chelating (10.09–28.49 mg EDTAE/g. The extracts of A. djalonensis collected from Mafiblé, especially those of stem bark, contained higher level of total bioactive contents compared to Prikro extracts, detected by high‐performance liquid chromatography with photodiode‐array detection (HPLC‐DAD). Only the methanolic extracts irrespective of plant parts/location, showed inhibition against acetylcholinesterase (1.42–2.12 mg galantamine equivalent (GALAE)/g), while only the stem bark methanolic extract of A. djalonensis from Mafiblé was found to inhibit butyrylcholinesterase (0.65 mg GALAE/g). Thus, findings from this study could be useful for better application of the medicinal benefits from this plant.

Protective effect of Arum maculatum against dextran sulfate sodium induced colitis in rats

ABSTRACT Ulcerative colitis (UC) is an inflammatory disease of the large intestine that is characterized by diarrhea, bloody stools, abdominal pain and mucosal ulceration. UC is treated with nonsteroidal anti-inflammatory drugs, corticosteroids or immunosuppressants, but long-term use of these drugs can cause adverse effects. Arum maculatum is used as a traditional treatment for digestive system disorders, but its use for treatment of UC has not been investigated rigorously. We investigated the possible protective effect of a methanol extract of A. maculatum against dextran sulfate sodium (DSS) induced experimental UC in rats. Total phenolic and flavonoid contents of the extract were 32.919 ± 1.125 mg gallic acid equivalent (GAE)/g and 52.045 ± 7.902 µg rutin equivalent (RE)/mg, respectively. The half-maximal inhibitory concentration (IC50) for the extract was 105.76 µg/ml according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay. Effects of A. maculatum extract on UC induced by DSS were assessed both macroscopically and histologically. We also investigated effects of A. maculatum extract on malondialdehyde (MDA) levels and the oxidative stress index (OSI) in normal rats and rats with UC. We found that treatment with A. maculatum extract protected the colon against DSS induced UC in a dose-dependent manner.

Comparison of the effect of extraction methods on the waste cotton (Gossypium hirsutum L ) flowers: metabolic profile, bioactive components, antioxidant, and α-amylase inhibition.

Waste cotton flowers, as a by-product of cotton cultivation, are enriched with bioactive substances that render them a promising natural source of health-promoting benefits. In this study, ultrasound-assisted extraction (UAE), subcritical water extraction (SWE) and conventional extraction (CE) approaches were applied to extract bioactive compounds from waste cotton flowers, and metabolic profile, bioactive components, antioxidant, and α-amylase inhibition of different extractions were systematically analyzed and compared. It was observed that the UAE and CE extract had similar metabolic profiles compared with SWE. The flavonoids and amino acids and derivatives were more prone to be extracted by UAE and CE, while phenolic acids tended to accumulate in SWE extract. The UAE extract had the highest amounts of total polyphenols (214.07 mg GAE/g DW) and flavonoids (33.23 mg RT/g DW) as well as the strongest inhibition on oxidation (IC50 =10.80 μg/mL) and α-amylase activity (IC50 =0.62 mg/mL), indicating that chemical composition was closely related to biological activity. Additionally, microstructures and thermal behaviors of the extract were investigated and highlighted the ability of UAE. Overall, it can be concluded that UAE is an efficient, green, and economical extraction method to produce bioactive compounds from cotton flowers, and the UAE extracts could be used in food and medicine industries because of their high antioxidant and α-amylase inhibitory activity. This study provides scientific basis for the development and comprehensive utilization of cotton by products. This article is protected by copyright. All rights reserved.

Nutrient composition and polyphenol profile of annual sunflower leaves (Helianthus annuus L.)

Goal. To study the nutritional value and polyphenolic composition of annual sunflower leaves (Helianthus annuus L.) for their further use as a phytogenic antioxidant additive for poultry feed. Methods. The chemical composition of phytoraw material (crude protein, crude fat, crude fiber, calcium, phosphorus, vitamin E, and b-carotene) was evaluated according to generally accepted met­hods. The quantitative content of individual groups of polyphenols was determined spectrophotometrically: the volume of hydroxycinnamic acids — in terms of chlorogenic acid, the volume of phenolic compounds — in gallic acid, flavonoids — in rutin. Results. It was established that sunflower leaves had a high content of b-carotene (260.2–291.6 µg/g) and the natural antioxidant vitamin E (37.4%). The protein content was fixed at the level of 16.2%, the fiber content was 4.2%. A significant amount of calcium and phosphorus accumulated in sunflower raw materials — 2.03% and 0.28%, respectively. The total content of hydroxycinnamic acids in terms of chlorogenic acid in sunflower leaves was 3.15%, the total content of phenolic compounds in terms of gallic acid was 1.60%, and flavonoids in rutin equivalent was 1.78%. Conclusions. The presence of polyphenolic compounds and the confirmed nutritional value of sunflower leaves give reasons to consider it not only as a valuable component of feed but also as a source of natural antioxidants.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Ranunculaceae) – a critically endangered endemic species growing in the Turkish flora

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir &amp;amp; Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.

Phytochemical and biological activity profiles of Thymbra linearifolia: An exclusively native species of Libyan Green mountains

Thymbra linearifolia, a Green Mountain endemic Libyan plant species belonging to the family Lamiaceae, was investigated as the plant's first documented phytochemical and biological evaluation report.The plant is used for bronchitis, cough, asthma, and gastrointestinal ailments, including dyspepsia, colic pain, and diarrhea.The phenolic and flavonoid contents of T. linearifolia were analyzed qualitatively and quantitatively by LC-MS analysis and spectrophotometric assays. These analyses revealed the presence of 60.67 mg/g of GAE (Gallic Acid Equivalents) and 26.79 mg/g of RE (Rutin Equivalents) of the phenolics and flavonoids, respectively, in the aerial parts of the plants’ ethanolic extract. Cyanidin-3-O-rutinoside (2.26 %), pelargonidin-3-O-rutinoside (2.36 %), gallocatechin (2.94 %), taxifolin (1.22 %), kaempferol-3-O-rutinoside (0.81 %), quercetin-3-O-retinosie (1.20 %), luteolin (0.99 %), apigenin (2.0 %), dimethoxy luteolin (2.97 %), and pinobanksin-3-O-acetate (2.56 %) were detected to be the major flavonoids, whereas the syringic acid hexoside (1.38 %), and rosmarinic acid (2.54 %) were among the most abundant phenolic acids in the plant’s extract. The DPPH scavenging activity was measured at an IC50 value of 34.48 μg/mL. In addition,potential reducing, metal chelating, and free radical scavenging potentials were exhibited in the in vitro assays, i.e., ABST, ORAC, FRAP, and MC. The remarkable antioxidant activity of the plant was attributed to its phenolic and flavonoid contents. The plant extract had strong cytotoxic effects on three types of cancer cell lines, i.e., MCF-7, HepG2, and Panc-1, with IC50 values of 24.023, 22.94, and 33.30 μg/mL, respectively. Furthermore, the plant extract also exhibited cytotoxic effects greater than the standard anticancer drug, doxorubicin, against all the tested cell lines. The in-silicobinding studies also demonstrated that the phytochemical constituents of T. linearifolia have a high binding affinity against the epidermal growth factor receptor (EGFR) protein, which supported the anticancer potentials of the plant.The antimicrobial assay conducted with the well-diffusion method revealed the moderate effects of the plant’s extract against Bacillus subtilis, Staphylococcus aureus, Saccharomyces cerevisiae, and Candida albicans. However, weak antimicrobial effects were recorded against the Gram-negative bacteria, Escherichia coli, and Salmonella typhimurium. These findings corroborated the immense health benefits of the plant, and substantiatedthe medicinal value claims of the plant, which is currently under use in different forms as part of the locals’ folk-medicinal chest.

Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential.

Despite the common use of Potentilla L. species (Rosaceae) as herbal medicines, a number of species still remain unexplored. Thus, the present study is a continuation of a study evaluating the phytochemical and biological profiles of aqueous acetone extracts from selected Potentilla species. Altogether, 10 aqueous acetone extracts were obtained from the aerial parts of P. aurea (PAU7), P. erecta (PER7), P. hyparctica (PHY7), P. megalantha (PME7), P. nepalensis (PNE7), P. pensylvanica (PPE7), P. pulcherrima (PPU7), P. rigoi (PRI7), and P. thuringiaca (PTH7), leaves of P. fruticosa (PFR7), as well as from the underground parts of P. alba (PAL7r) and P. erecta (PER7r). The phytochemical evaluation consisted of selected colourimetric methods, including total phenolic (TPC), tannin (TTC), proanthocyanidin (TPrC), phenolic acid (TPAC), and flavonoid (TFC) contents, as well as determination of the qualitative secondary metabolite composition by the employment of LC-HRMS (liquid chromatography-high-resolution mass spectrometry) analysis. The biological assessment included an evaluation of the cytotoxicity and antiproliferative properties of the extracts against human colon epithelial cell line CCD841 CoN and human colon adenocarcinoma cell line LS180. The highest TPC, TTC, and TPAC were found in PER7r (326.28 and 269.79 mg gallic acid equivalents (GAE)/g extract and 263.54 mg caffeic acid equivalents (CAE)/g extract, respectively). The highest TPrC was found in PAL7r (72.63 mg catechin equivalents (CE)/g extract), and the highest TFC was found in PHY7 (113.29 mg rutin equivalents (RE)/g extract). The LC-HRMS analysis showed the presence of a total of 198 compounds, including agrimoniin, pedunculagin, astragalin, ellagic acid, and tiliroside. An examination of the anticancer properties revealed the highest decrease in colon cancer cell viability in response to PAL7r (IC50 = 82 µg/mL), while the strongest antiproliferative effect was observed in LS180 treated with PFR7 (IC50 = 50 µg/mL) and PAL7r (IC50 = 52 µg/mL). An LDH (lactate dehydrogenase) assay revealed that most of the extracts were not cytotoxic against colon epithelial cells. At the same time, the tested extracts for the whole range of concentrations damaged the membranes of colon cancer cells. The highest cytotoxicity was observed for PAL7r, which in concentrations from 25 to 250 µg/mL increased LDH levels by 145.7% and 479.0%, respectively. The previously and currently obtained results indicated that some aqueous acetone extracts from Potentilla species have anticancer potential and thus encourage further studies in order to develop a new efficient and safe therapeutic strategy for people who have been threatened by or suffered from colon cancer.

Chemical Characterization of Different Extracts of Justicia secunda Vahl and Determination of Their Anti-Oxidant, Anti-Enzymatic, Anti-Viral, and Cytotoxic Properties.

Justicia secunda Vahl. is a traditional medicinal plant in tropical regions, including West Africa. The present study examined the chemical profiles and biological properties of J. secunda extracts obtained with different solvents (dichloromethane, ethyl acetate, methanolic and aqueous: macerated and infused). Chemical components were characterized by liquid chromatography-mass spectrometry (LC-MS), and over 50 compounds were identified, including flavonoids, phenolic acids, and alkaloids. Antioxidant, enzyme inhibitory, cytotoxic, and antiviral properties were selected as biological properties. Total phenolic and flavonoid contents in methanol (58.07 mg gallic acid equivalent (GAE)/g and 13.07 mg rutin equivalent (RE)/g) and water (infused) (36.34 mg GAE/g and 8.52 mg RE/g) were higher than in other extracts. Consistent with the levels of total bioactive components, the methanol and water extracts exhibited stronger antioxidant abilities. However, the dichloromethane and ethyl acetate extracts were more active on α-amylase and α-glucosidase than other extracts. Aqueous extracts exerted selective anticancer properties toward human pharyngeal cancer cell lines, whereas the methanolic extract decreased the human herpesvirus type-1 (HHV-1) infectious titer by 2.16 log and the viral load by 1.21 log. Overall, J. secunda could be considered a multifunctional bioactive raw material in the preparation of potent applications to manage diseases related to oxidative stress, including cancer, diabetes, and Alzheimer's.

Evaluation of seasonal variation, effect of extraction solvent on phytochemicals and antioxidant activity on Rosmarinus officinalis grown in different agro-ecological zones of Kiambu County, Kenya

BackgroundRosemary (Rosmarinus officinalis) is a commonly used culinary herb with great potential applications in the pharmaceutical, food, and cosmetics industries because of its reported bioactive phytochemicals and antioxidant properties. The purpose of the study was to investigate the effect of seasonal variations in different agro-ecological zones (AEZs) on the phytochemical content and corresponding antioxidant activities of R. officinalis, to ascertain the best growth period at which the plant possesses the highest phytochemical components and most potent antioxidant property. The study also aimed at comparing different extraction solvents to establish the best extraction system for the bioactive compounds.MethodsThe leaves of R. officinalis were harvested from six purposively selected sites in four agro-ecological zones of Kiambu County, Kenya both in the wet and the dry seasons. Phytochemicals were extracted in 80% methanol, 80% ethanol, and distilled water. Total phenolic content (TPC), total flavonoids content (TFC), and total tannins content (TTC) were measured spectrophotometrically as gallic acid equivalent (GAE), rutin equivalent (RUTE) and tannic acid equivalent (TAE), respectively. The antioxidant activities were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP). The 80% ethanolic solvent was used to compare the phytochemical content and corresponding antioxidant activities of R. officinalis leaf samples collected from two consecutive seasons in different agro-ecological zones.ResultsThe solvents showed no significant difference (P > 0.05) in TPC with ethanol repotting the highest followed by methanol and water ranging from 39.71 ± 6.77, 24.91 ± 5.15 and 24.91 ± 7.30 (mg/g GAE), respectively. The aqueous TFC (117.22 ± 3.64 mg/g RUTE) was the highest followed by ethanolic and methanolic with 34.72 ± 2.13 and 16.86 ± 2.80 mg/g RUTE, respectively. The TTC of water, methanol, and ethanol extracts were; 19.88 ± 4.59, 15.02 ± 1.25, and 4.27 ± 1.48 mg/g TAE, respectively. The DPPH activity between methanol and ethanol extracts showed no significant difference. The FRAP activity also showed no significant difference (P > 0.05) among the three solvents. There were significant differences between the wet and dry seasons in the phytochemical content. There was no recorded significant difference in the DPPH activity between the dry and wet season in all AEZs. FRAP was significantly higher in the dry season than the wet season for R. officinalis leaves harvested in all agro-agroecological zones except Thika. There were significant differences in phytochemical content and antioxidant activity between the agro-ecologicalzones (p < 0.05) except for the TFC.ConclusionsThe data obtained from this study demonstrated that hydro-alcoholic /methanolic and aqueous maceration systems extracted bioactive compounds from R. officinalis with high potential for applications in industries. The R. officinalis from different agro-ecological zones contained variable phytochemical composition, suggesting that geographical location and climatic conditions influence the biosynthesis and accumulation of secondary metabolites and other bioactive compounds. The data provided in this study will be crucial for processors to select the optimal harvesting season for the extraction of desired bioactive compounds from Rosmarinus officinalis.

Antioxidant and antimicrobial characteristics of ethyl acetate polar fractions from walnut green husk.

Walnut green husk (WGH) is rich in natural compounds and is valued as a potential source of antioxidant and antimicrobial properties. In this study, the antioxidant and antimicrobial activities of petroleum ether polar fraction, dichloromethane polar fraction, ethyl acetate polar fraction (EAPF), and n-butanol polar fraction from WGH were analyzed. The results showed that EAPF exhibited the highest total flavonoid content (65.74±1.01mg rutin equivalents [RE]/g dry weight [DW]) and total phenol content (48.73±1.09mg gallic acid equivalent [GAE]/g DW), with the highest 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical (•OH), and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonate scavenging activity compared with other fractions. EAPF also showed good antibacterial activity against Escherichia coli and Bacillus cereus vegetative cells, with a diameter of inhibition zones of 33.5 and 37.6mm, respectively, a minimum inhibitory concentration of 31.25mg/ml and a minimum bactericidal concentration of 62.5mg/ml, which inhibited the growth of both bacteria. Analysis of the antibacterial mechanism demonstrated that EAPF damaged the integrity of the cell membrane, increased the membrane permeability, and triggered the leakage of intracellular material. In addition, ultrahigh performance liquid chromatography-tandem with mass spectrometry analysis revealed that 8 polyphenols and 14 flavonoids were mainly present in EAPF, such as chlorogenic acid (C16 H18 O9 ), gallic acid (C7 H6 O5 ), vanillic acid (C8 H8 O4 ), ferulic acid (C10 H10 O4 ), epicatechin (C15 H14 O6 ), catechin (C15 H14 O6 ), hesperetin (C16 H14 O6 ), naringenin (C15 H12 O5 ), hyperin (C21 H20 O12 ), luteolin (C15 H10 O6 ), and so on. Therefore, WGH had the potential to be developed as a natural antioxidant and antibacterial material. PRACTICAL APPLICATION: Our work indicates that WGH contains abundant flavonoids and polyphenols compounds. Therefore, the plant byproducts like WGH may have a promising application as a source of antimicrobial and antioxidant additives.

Assessment of the Bioaccessibility of Elaeagnus angustifolia L. Flour and Its Use in Cracker Formulation

In this study, in vitro digestion of polyphenols of oleaster (Elaeagnus angustifolia L.) flour (OF) and the potential use of OF in cracker production were studied. The results showed that 21.90mg gallic acid equivalent (GAE)/g dry matter (DM) of total polyphenol (TP), 28.15mg rutin equivalent (RE)/g DM of total flavonoid (TF) and 7709.13mmol ascorbic acid equivalent (AAE)/100g DM of antioxidant capacity (AC) were found in OF. The bioaccessibility and content of the OF polyphenols and their AC during in vitro digestion varied depending on the digestion stage. The stability of the OF polyphenols was higher in gastric stage (41.92%) than intestinal one (34.01%). OF was used to replace flour in the cracker formulation at the levels of 5, 10 and 15% (w/w) and increased the TP contents and AC of the crackers compared to the sample without OF (control). The addition of OF to the cracker dough increased the bioaccessibility of the cracker polyphenols. After digestion, bioaccessibilities of enriched crackers (107.90-126.81%) were found higher than that of control sample (93.73%). However, as the level of OF in cracker increased, bioaccessibility of polyphenols decreased. The results generally indicated that oleaster has a good potential for the enrichment of foods.

Identification of Secondary Metabolites of Interest in Pleurotus djamor Using Agave tequilana Bagasse

Agro-industrial residues represent more than 60% of organic wastes worldwide, which could be used to generate other by-products or to be incorporated into other production chains. For example, bagasse is a waste from the tequila industry in Mexico that could be implemented for mushroom cultivation. Additionally, the substrate influences the growth, development, and production of secondary metabolites of fungi. This work presents a comparative experiment that studies the metabolite production in Pleurotus djamor mushrooms on agave bagasse and barley straw (traditional substrate). The biological efficiency (BE), yield, phenolics and flavonoids, antioxidant capacity, tannins, and the identification of low molecular weight metabolites were evaluated. Five treatments were proposed according to the following mixtures of agave bagasse: barley straw: T1 (1:0), T2 (3:1), T3 (1:1), T4 (1:3), and T5 (0:1). T2 had the highest yield (13.39 ± 3.23%), BE (56.7 ± 13.71%), and flavonoids (44.25 mg rutin equivalent (RE)/g); T3 obtained the highest phenol content (230.27 mg GAE/g); and T1 the highest tannins content (0.23 mg (+) catechin equivalent (CE)/g). Finally, T1 and T5 are the ones that present the greatest number of primary metabolites, including hydroxycitric acid, 2-deoxy-D-galactose, D-mannose, paromomycin, palmitic acid, pyrrole, mannitol, and DL arabinose, while in T2, T3, and T4 only two chemical compounds were found present (palmitic acid and pyrrole in T2, silicic acid and pyrrole in T3 and 2-deoxy-D-galactose and quinoline in T4). The cultivation substrate influences the concentration of bioactive molecules in the fruiting bodies of P. djamor. Additionally, P. djamor's degradation of agave bagasse residue generates a potential application for agro-industrial residue management at a low cost.

HR-LCMS assisted phytochemical screening of antioxidant, antibacterial activity of Priva cordifolia (L.f) Druce plant and molecular docking approach

Ethnomedicinal plants have been employed for treatments in phytotherapy due to the quality, effectiveness, and assurance of herbal therapy. The present study aims to assess the different extracts (16 extracts) obtained from foliage, stem bark, root, and seeds by increased polarity solvent-based on extraction and analyze the antioxidant properties of Priva cordifolia (L.f) Druce plant. Total polyphenols and flavonoids have been found to range from 20.60 ± 0.19–148.20 ± 1.52 mg/g as gallic acid equivalent (GAE) and 7.31 ± 0.09–350.46 ± 12.80 mg/g as rutin equivalent (RE) respectively. All the extracts have been assessed for DPPH, ABTS scavenging radical and FRAP activity. Among these, methanol and acetone extract of the foliage showed excellent activity. The foliage extract was further fractioned and examined for antioxidant and antibacterial activity. A total of 27 metabolites were identified with the help of HRLC-MS. All the identified metabolites were tested for pharmacophore model, ADME, Blood-Brain Barrier, and Molecular docking analysis. In this insilico method, all compounds have given a good docking score and binding energy to the particular protein target, which ranges from − 6 to −10 against DNA gyrase protein (3G7B).The fraction was further evaluated for their cytotoxicity against normal cell L6 and revealed data showing insignificant toxicity. The foliage methanolic extract indicated a broad phytochemical in P. cordifolia and is yet to explore unknown vital constituents for the medicinal applications in the future.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Asteraceae) – a critically endangered endemic species growing in the Turkish flora

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir &amp;amp; Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.

Diverse polyphenol components contribute to antioxidant activity and hypoglycemic potential of mulberry varieties

Profiles of phenolic compounds, antioxidant activities, α-glucosidase inhibitory and anti-glycation activities of nine mulberry varieties were investigated. Twenty-three phenolic components were identified in the mulberry fruit. Chlorogenic was the predominant phenolic acid, while rutin was the most abundant flavonoid. In the colored mulberry fruits, the major anthocyanins were cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside. Furthermore, we provide the first report of taxifolin-O-glucoside and two p-coumaroyl-caffeoylquinic acid isomers from mulberry fruit. The total phenolic content (TPC) ranged from 4.12 to 35.50 mg gallic acid equivalents (GAE)/g, while the total flavonoid content (TFC) varied between 1.16 and 39.95 mg rutin equivalents (RE)/g. The total anthocyanin content (TAC) ranged from 7.44 to 25.84 mg/g. The Zisang NO 1 variety presented the highest TPC, TFC and TAC with values of 35.50 mg GAE/g, 39.35 mg RE/g and 25.84 mg/g, respectively. Accordingly, it was characterized by the highest antioxidant capacity with values of 238.3 μmol Trolox equivalents (TE)/g and 560.1 μmol TE/g for DPPH and FRAP assays, respectively. Additionally, Zisang NO 1 showed better α-glucosidase inhibitory activity and anti-glycosylation activity, which may be due to its diverse polyphenolic composition. The antioxidant activity and the hypoglycemic potential were significantly higher in the colored mulberry fruits compared to the white mulberry varieties.