Rumen Of Cows Research Articles

MT-MAG: Accurate and interpretable machine learning for complete or partial taxonomic assignments of metagenomeassembled genomes.

We propose MT-MAG, a novel machine learning-based software tool for the complete or partial hierarchically-structured taxonomic classification of metagenome-assembled genomes (MAGs). MT-MAG is alignment-free, with k-mer frequencies being the only feature used to distinguish a DNA sequence from another (herein k = 7). MT-MAG is capable of classifying large and diverse metagenomic datasets: a total of 245.68 Gbp in the training sets, and 9.6 Gbp in the test sets analyzed in this study. In addition to complete classifications, MT-MAG offers a "partial classification" option, whereby a classification at a higher taxonomic level is provided for MAGs that cannot be classified to the Species level. MT-MAG outputs complete or partial classification paths, and interpretable numerical classification confidences of its classifications, at all taxonomic ranks. To assess the performance of MT-MAG, we define a "weighted classification accuracy," with a weighting scheme reflecting the fact that partial classifications at different ranks are not equally informative. For the two benchmarking datasets analyzed (genomes from human gut microbiome species, and bacterial and archaeal genomes assembled from cow rumen metagenomic sequences), MT-MAG achieves an average of 87.32% in weighted classification accuracy. At the Species level, MT-MAG outperforms DeepMicrobes, the only other comparable software tool, by an average of 34.79% in weighted classification accuracy. In addition, MT-MAG is able to completely classify an average of 67.70% of the sequences at the Species level, compared with DeepMicrobes which only classifies 47.45%. Moreover, MT-MAG provides additional information for sequences that it could not classify at the Species level, resulting in the partial or complete classification of 95.13%, of the genomes in the datasets analyzed. Lastly, unlike other taxonomic assignment tools (e.g., GDTB-Tk), MT-MAG is an alignment-free and genetic marker-free tool, able to provide additional bioinformatics analysis to confirm existing or tentative taxonomic assignments.

Effect of Processed Rumen Digesta with Probiotics on Eggs Quality Characteristics of Khaki Campbell Ducks (Anas Platyrhynchos Domesticus)

Khaki Campbell is a duck with a dual purpose, as a producer of meat and egg, although its role as laying is more prominent. The aim of the present study is to examine feed ingredients for ducks (poultry) originating from abattoir waste that does not compete with humans. Feed is the largest component that affects poultry production since feed expenses occupy at least 60 - 70% of the production cost. The examined feed ingredient was the rumen digesta of the cow that was collected from the slaughterhouse and it is recommended to participate at 10 - 15% in rations, so as not to cause adverse side effects. The method of addition of probiotics to the feed of processed cow rumen serves to improve the growth rate, feed conversion efficiency, egg production, and livestock health. Measurements in the form of the amount of raw feed balance with processed rumen digesta substitution feed on the productivity of duck eggs. The conclusion of this study that the partial replacement of processed rumen content feeds up to 15% in Khaki Campbell ducks, did not give a noticeable difference (p>0.05) on egg weight, egg shape index, egg white index, yolk index, Haugh Unit, and egg yolk color. The main finding is that processed beef rumen content at the level of 10-15% can be used as a diet ingredient for ducks without affecting egg quality characteristics.

Extracellular Expression of Feruloyl Esterase and Xylanase in Escherichia coli for Ferulic Acid Production from Agricultural Residues.

There is still a large amount of ferulic acid (FA), an outstanding antioxidant, present in agricultural residues. Enzymatic hydrolysis has been regarded as the most effective way to release FA. This present study therefore selected feruloyl esterase (FAE) and xylanase (XYN) from the metagenomes of a cow rumen and a camel rumen, respectively, for their recombinant expression in Escherichia coli BL21 and further application in releasing FA. After screening the candidate signal peptides, the optimal one for each enzyme, which were selected as SP1 and SP4, respectively, was integrated into the vectors pET22b(+) and pETDuet-1. Among the generated E. coli strains SP1-F, SP4-X, and SP1-F-SP4-X that could express extracellular enzymes either separately or simultaneously, the latter one performed the best in relation to degrading the biomass and releasing FA. Under the optimized culture and induction conditions, the strain SP1-F-SP4-X released 90% of FA from 10% of de-starched wheat bran and produced 314.1 mg/L FA, which was deemed to be the highest obtained value to the best of our knowledge. This result could pave a way for the re-utilization of agricultural residues and enhancing their add-value.

تأثير إضافة محتوى الكرش للأبقار على صفات الزيادة الوزنية والمعدلات النسبية والنوعية لأسماك الكارب العشبي Ctenopharyngodon idella

The study was conducted in the fish laboratory, College of Agricultural Engineering Sciences, University of Baghdad, with the aim of using the contents of the rumen of cows supported with the prebiotic as a diet for grass carp fish. In the research, 190 fish were used, with an average starting weight of 40 g. 15 fingerlings, the fish were fed on four types of rations equal in protein, after crushing and grinding the materials and adding the contents of the rumen at rates of 10, 15and 20, fortified with a prebiotic of 2 g/kg, except for the control ration free of addition. The results of the research indicated that the second treatment had a significant superiority over all treatments in terms of body weight rates, weight gain, relative and specific weight rates, feed conversion ratio, feed conversion efficiency, and apparent digestion coefficient, followed by the third treatment, then the fourth treatment, and for all durations of the experiment. The results also indicated that the fourth treatment had a significant superiority in the characteristic of the time for the appearance of droppings after 10 h had passed from the presentation of the diet, followed by the third treatment and then the second treatment .The results also indicated that feed consumption increased significantly for the second treatment, followed by the third treatment, and then the fourth treatment.

Utilization of Cow Rumen into Biogas Using a Fixed Dome Reactor as an Alternative Energy in Deli Serdang Regency

Biogas is renewable energy that can be used as substitute of LPG and generator fuel. The utilization of waste into biogas has been implemented in Tadukan Raga Village, Sinembah Tanjung Muda (STM) Hilir District, Deli Serdang Regency since 2018 by building a facility for processing biomass into biogas by utilize rumen waste and cow dung at pilot scale. This facility has been handed over by the Ministry of Environment and Forestry to the community to manage independently. However, there is a problem in the lack of understanding of the community in the management, development and maintenance of this facility. This problem resulted in the abandonment of the biogas plant so that production and operations not running and some equipment requiring repairs. The purpose of this program is to strengthen and empower observer groups and users of biogas production facilities in Tadukan Raga Village. Therefore it is necessary to carry out: an analysis of the sustainability of the biogas plant program and the use of biogas as an alternative to overcoming livestock manure pollution in the program of Desa Binaan USU 2022, repair of biogas reactor and piping and the use of biogas stoves modified from LPG stoves.

Maca (Lepidium meyenii): In Vitro Evaluation of Rumen Fermentation and Oxidative Stress

The aim of this study was to investigate the chemical composition of three maca (Lepidium meyenii Walp.) ecotypes (yellow, black, and red) and their in vitro fermentation characteristics and antioxidant effects on cow rumen liquor. The three ecotypes were added to a total mixed ratio (TMR) in different doses (0, 150, and 300 mg/g) and incubated in vitro under anaerobic conditions for 120 h. Methane production was recorded after 24 h of incubation. Antioxidant status and degree of lipid peroxidation were also evaluated after 24 and 120 h of incubation with the fermentation liquor. An analysis of the chemical composition showed high concentrations of non-structural carbohydrates in all maca ecotypes, particularly in the yellow ecotypes. Moreover, despite an increase in gas production, it seems that the TMR supplemented with each maca ecotype, particularly at the highest dose, increases the amount of volatile fatty acids and reduces methane production. Finally, the addition of maca can induce an antioxidant effect. Our findings suggest that the three ecotypes of maca are rich in non-structural carbohydrates which affect the in vitro fermentation kinetics and reduce methane production.

A thermotolerant yeast from cow's rumen utilize lignocellulosic biomass from wheat straw for xylanase production and fermentation to ethanol

For recycling agricultural wastes into the production of biofuel, fermentation technology is currently gaining more attention. Bioethanol is a widely used biofuel, however in industries, the fermentation of hemicellulosic feedstock is challenging since fermenting yeasts consume fewer xylose. To enhance the availability of renewable biofuels, Pioneers are keen to develop industrial strains that assimilate xylose along with glucose. In attempt to eliminate the obstacle, xylanolytic yeast with enhanced xylanase production were investigated. A thermotolerant yeast species, Pichia kudriavzevii strain SVMS2019, isolated from the cow's rumen was studied for xylanase production under solid-state fermentation with simultaneous degradation and saccharification of alkali-treated wheat straw biomass for the liberation of xylose sugars and then conversion into bioethanol. After statistical optimization through response surface methodology, the xylanase enzyme yield was enhanced up to 2.23 fold; the optimum conditions were 30 °C, pH 3.5, 72 h of incubation, and 1.5% of pretreated wheat straw to produce 273.02 IU/ml xylanase activity. The maximum of crude xylanase activity was shown at 50 °C and pH 6. The fermentation of xylose to ethanol by Pichia kudriavzevii SVMS2019, bioethanol was obtained at a maximum of about 3.18% at 42 °C in 72 h intervals. Through the novel source, we have obtained the strain that has a significant of hemicellulosic enzymatic saccharification with 2.23 fold increase in xylanase production yield through statistical optimization and produce bioethanol. While xylanolytic activity of yeast has been optimized, potential benefit of cellulosic hydrolytic activity also need to be studied to achieve the co-utilization of glucose and xylose.

The impact of ruminal fluid lipolytic substances on the reduction of oils and fats in wastewater in the dairy industry and its influence on business decisions

The dairy industry is known as one of the major contributors to wastewater generation in terms of organic load value and effluent volume. The microbial ecosystem of the rumen that in Ecuador is considered as a waste from the slaughter mainly of cows in the municipal beds comprises at least 30 predominant bacterial species. The purpose of this work was to determine the ability of lipolytic bacteria extracted from cow rumen for the degradation of wastewater oils and fats generated by the dairy industry. Experimental tests were performed for the isolation of lipolytic bacteria from the rumen from three culture media: tributyrin, lecithinase and tr ibutirine with 5 and 10% lecithinase under aerobic and anaerobic conditions. Tributyrin under anaerobic conditions was considered the most suitable culture medium for the massification of lipolytic bacteria from rumen fluid. The experimental units were conditioned also based on pH, temperature and Biochemical Oxygen Demand (BOD5). 3 pH regulating agents of the experimental units were tested: Sodium Carbonate, Sodium Bicarbonate and Bicarbonate. As pH regulating agent, sodium carbonate was used, which allowed reaching a value of 6.5 with only 5mL of dosage. Three moments of analysis were considered: the initial moment (or days), an intermediate moment (28 days) and the final moment (56 days). The ability of lipolytic bacteria to reduce the concentration of oils and fats in dairy industry wastewater in 56 days was 48.49% compared to the initial concentration, that is, 46.4 mg / L reduced to 22.5mg / L, so it was shown that its applicability is highly efficient in proportion to the research conditions. The high efficiency of these bacteria was directly related to the conditioning of pH and BOD5, based on the results that were generated by applying the correlation matrix and the Chi-square method. The analysis of the results obtained does not promote the biotechnological treatment of industrial wastewater, but also promotes the use of waste as a source of degrading organisms.

Evaluation of the capacity of rumen fluid lipolytic substances for the reduction of oils, wastewater fats in the dairy industry and their impact on business decision making

The dairy industry is known as one of the major contributors to wastewater generation in terms of organic load value and effluent volume. The microbial ecosystem of the rumen that in Ecuador is considered as a waste from the slaughter mainly of cows in the municipal beds comprises at least 30 predominant bacterial species. The purpose of this work was to determine the ability of lipolytic bacteria extracted from cow rumen for the degradation of wastewater oils and fats generated by the dairy industry. Experimental tests were performed for the isolation of lipolytic bacteria from the rumen from three culture media: tributyrin, lecithinase and tr ibutirine with 5 and 10% lecithinase under aerobic and anaerobic conditions. Tributyrin under anaerobic conditions was considered the most suitable culture medium for the massification of lipolytic bacteria from rumen fluid. The experimental units were conditioned also based on pH, temperature and Biochemical Oxygen Demand (BOD5). 3 pH regulating agents of the experimental units were tested: Sodium Carbonate, Sodium Bicarbonate and Bicarbonate. As pH regulating agent, sodium carbonate was used, which allowed reaching a value of 6.5 with only 5mL of dosage. Three moments of analysis were considered: the initial moment (or days), an intermediate moment (28 days) and the final moment (56 days). The ability of lipolytic bacteria to reduce the concentration of oils and fats in dairy industry wastewater in 56 days was 48.49% compared to the initial concentration, that is, 46.4 mg / L reduced to 22.5mg / L, so it was shown that its applicability is highly efficient in proportion to the research conditions. The high efficiency of these bacteria was directly related to the conditioning of pH and BOD5, based on the results that were generated by applying the correlation matrix and the Chi-square method. The analysis of the results obtained does not promote 3626the biotechnological treatment of industrial wastewater, but also promotes the use of waste as a source of degrading organisms.

Quality of cow dung composting fertilizer with additional starter solution of cow rumen contents

RPH Kedurus produces livestock solid waste in the form of cow dung as much as 750 kg/day and the content of cow rumen is 2 tons/day. The livestock solid waste can be processed by turning it into compost using the takakura composting method. This study aims to determine the characteristics of the compost according to the specified parameters (temperature, pH, moisture content, organic c, nitrogen, phosphorus, potassium, and the C/N ratio) and to determine the dose of use of a good cow rumen content solution to produce compost according to the quality standard of SNI 19-7030-2004. In this study, the treatments used were P0 with a composition of 3 kg of cow dung and 1 kg of rice husks, P1 with a composition of P0 and 100 mL of starter solution of cow rumen contents, P2 with a composition of P0 and 200 mL of starter solution of cow rumen contents, and P3 with a composition of P0 and 300 mL of starter solution for bovine rumen contents. Overall, the characteristics produced by the four treatments met the quality standards, while the optimum dosage for the use of rumen contents was found in treatment P2 with the use of 200 mL of starter bovine rumen contents and produced a temperature of 29 °C, pH 7.4, water content 14.2%, C. -organic 67 %, nitrogen 4.26 %, phosphorus P2O5 0.37 %, potassium K2O 2.21 %, and C/N ratio 15.7. The compost fertilizer produced can be used as an alternative organic fertilizer to be developed.

Isolation and pan-genome analysis of Enterobacter hormaechei Z129, a ureolytic bacterium, from the rumen of dairy cow.

Urea is an important non-protein nitrogen source for ruminants. In the rumen, ureolytic bacteria play critical roles in urea-nitrogen metabolism, however, a few ureolytic strains have been isolated and genomically sequenced. The purpose of this study was to isolate a novel ureolytic bacterial strain from cattle rumen and characterize its genome and function. The ureolytic bacterium was isolated using an anaerobic medium with urea and phenol red as a screening indicator from the rumen fluid of dairy cattle. The genome of isolates was sequenced, assembled, annotated, and comparatively analyzed. The pan-genome analysis was performed using IPGA and the biochemical activity was also analyzed by test kits. A gram-positive ureolytic strain was isolated. Its genome had a length of 4.52 Mbp and predicted genes of 4223. The 16S rRNA gene and genome GTDB-Tk taxonomic annotation showed that it was a novel strain of Enterobacter hormaechei, and it was named E. hormaechei Z129. The pan-genome analysis showed that Z129 had the highest identity to E. hormaechei ATCC 49162 with a genome average nucleotide identity of 98.69% and possessed 238 unique genes. Strain Z129 was the first E. hormaechei strain isolated from the rumen as we know. The functional annotation of the Z129 genome showed genes related to urea metabolism, including urea transport (urtA-urtE), nickel ion transport (ureJ, tonB, nixA, exbB, exbD, and rcnA), urease activation (ureA-ureG) and ammonia assimilation (gdhA, glnA, glnB, glnE, glnL, glsA, gltB, and gltD) were present. Genes involved in carbohydrate metabolism were also present, including starch hydrolysis (amyE), cellulose hydrolysis (celB and bglX), xylose transport (xylF-xylH) and glycolysis (pgi, pgk, fbaA, eno, pfkA, gap, pyk, gpmL). Biochemical activity analysis showed that Z129 was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidase, and pyrrolidone arylaminase, and had the ability to use D-ribose, L-arabinose, and D-lactose. Urea-nitrogen hydrolysis rate of Z129 reached 55.37% at 48 h of incubation. Therefore, the isolated novel ureolytic strain E. hormaechei Z129 had diverse nitrogen and carbon metabolisms, and is a preferred model to study the urea hydrolysis mechanism in the rumen.

Analysis of Added Value of Cow Rumen into Compost Fertilizer in Kendari City Slaughterhouses (RPH) (Case Study of Ikhlas RPH Farmer Group Business)

This study aims to analyze the added value of processing cow rumen into compost at the Kendari City Slaughterhouse (RPH). This research was conducted from August 2021 to April 2022 using a case study method on the Ikhlas Farmer Group business, with eight farmer group members who serve as informants. The variables of this study were the respondent's identity and the characteristics of processing cow rumen contents into compost. Analysis of the data used is quantitative data analysis with the Hayami method. The results showed that the added value from processing cow rumen into compost in Poasia District, Anggoeya Village, Kendari City was IDR. 5.511/kg, which has a high ratio with added value to product value (44%) and profit of IDR. 4,574/kg. with a profit rate of 83%.

INFLUENCE OF MINERAL-VITAMIN PREMIX ON INDIVIDUAL LINKS OF METABOLISM AND PRODUCTIVITY OF COWS

The recipe of mineral-vitamin premix, made on the basis of salts of mineral elements (Cuprum, Zinc, Cobalt, Iodine and Selenium) and fat-soluble vitamins (A and D), which are deficient in the Pre-Carpathian zone, has been developed and its influence on individual metabolic links and productivity of dairy cows has been investigated. The experiment was conducted on two groups of highly productive cows of Simental breed, analogues in origin and productivity in the summer-pasture period of keeping. The main diet for animals of both groups consisted of pasture grass, mowed mass of cereal-bean mixtures of green conveyor, hay cereal-mixed grass, molasses and standard feed K 60-32-89 with premix P 60-5M. The experimental group received a similar amount of this feed, but with an experimental mineral-vitamin premix. It was found that in the herbal-concentrate type of diet in the control group, the deficiency of Cuprum, Zinc, Cobalt, Iodine and Selenium was 16.6, respectively; 13,2; 6,9; 26,2; 51.8%, and the deficiency of vitamins A and D - 60.0 and 16.3%. Introduction of mineral-vitamin premix into the compound feed, which contained salts of the specified elements deficient for the Pre-Carpathians, made it possible to better balance the rations according to the mineral part, which significantly influenced both the intensity of metabolic processes in the rumen and blood, and the milk productivity of cows and the quality of milk. In particular, the positive effect of its feeding on the level of fermentation, the activity of enzyme systems and the intensity of synthetic processes in the rumen of cows of the experimental group was noted. The study of the hematological picture showed a tendency to increase the level of red blood cells and hemoglobin in the blood of cows of the experimental group, an increase in the concentration of nitrogen fractions and urea (P < 0.01). Analyzing the results of research, it can be argued that ensuring the optimal level of mineral-vitamin nutrition led to an increase in the intensity of metabolic processes in the body of animals and therefore contributed to an increase in dairy productivity.
 The average daily yield of natural milk in the experimental group for 90 days of the accounting period was 21.6 kg and was 10.8% higher than in the control (19.5 kg). The chemical composition of milk of cows that received a mineral-vitamin premix as part of the feed was preferable. It showed a probable increase in the content of dry matter, total protein (P < 0.05) and calcium (P < 0.02).

Rumen fermentation and forage degradability in dairy cows offered perennial ryegrass, perennial ryegrass and white clover, or a multispecies forage

An experiment was conducted to compare the rumen fermentation and degradability of three forage treatments. A perennial ryegrass (PRG; Lolium perenne), perennial ryegrass and white clover (Trifolium pratense) (PRGWC) and multispecies forage (MULTI), containing two grass species; perennial ryegrass and timothy (Phleum pratense); two clover species; white clover and red clover (Trifolium repens); and two herb species; chicory (Cichorium intybus) and plantain (Plantago lanceolata), were compared. Six rumen fistulated lactating dairy cows were used in a replicated 3 × 3 Latin square experiment consisting of 3 periods lasting 21-days. Cows were allowed ad libitum access to fresh forage and offered 2.71 kg DM concentrate each day. During days 1 – 14 of each period, cows were acclimatized to dietary treatments before entering a 7 – day sampling period. Indwelling rumen pH probes were inserted into the rumen of each cow on day 15 and 16. Rumen fluid samples were taken on day 18 every 2 h for rumen volatile fatty acid and ammonia nitrogen (NH3N) analysis. Forage dry matter (DM) and crude protein (CP) degradability was assessed via in sacco incubation beginning on day 19 of each period. Treatment had no effect on dry matter intake (DMI) or milk yield. There was no difference in mean rumen pH between treatments. The rumen pH of cows offered MULTI was below pH 6.0 for a higher percentage of time than the other treatments, however the concentration of acetic acid in the rumen of cows was similar between treatments. Effective degradability (ED) of DM was higher (P = 0.003) for MULTI (0.77) compared to PRG (0.68) and PRGWC (0.66). The soluble CP fraction of MULTI and PRGWC was significantly lower than that of PRG (P = 0.007), though PRGWC had the highest rumen ammonia concentration of all treatments (P = 0.002). MULTI had the highest ED and rate of DM degradation in the rumen, and lowest soluble CP of all treatments. Multispecies forages can support milk production in lactating dairy cows; however, care must be taken to ensure adequate dietary NDF due to the low fiber concentration and highly degradable nature of the forage.

High-Dose Vitamin E Supplementation Can Alleviate the Negative Effect of Subacute Ruminal Acidosis in Dairy Cows.

The aim of this trial was to assess whether the supplementation of vitamin E (VE) in high-concentrate diets could improve the fermentation and blood metabolism in the rumen of dairy cows, thereby modulating the degree of the subacute ruminal acidosis (SARA) response and improving the performance. Seven Holstein cows (four fitted with ruminal cannulas) were fed three diets (total mixed rations) during three successive periods (each lasted for 18 d): (1) the control diet (CON); (2) a high-grain (HG) diet, which was the control diet supplied with a 15% finely ground wheat diet (FGW); and (3) a high-VE diet (HGE), which was the control diet provided with a 15% FGW and 12,000 IU of VE/head per day. The results indicated that VE was able to alleviate the reduction in the dry matter intake (DMI) and milk fat yield in cows caused by HG diets. The supplementation of VE significantly reduced the levels of lipopolysaccharide (LPS), histamine (HIS), and the total volatile fatty acid (TVFA) in the rumen. The supplementation of VE observably increased the antioxidant capacity of the milk and plasma. In addition, VE markedly reduced the plasma levels of endotoxin, HIS, and pro-inflammatory factors. The supplementation of VE significantly enriched the differential metabolites of the purine metabolism, cysteine, methionine metabolism, and ABC transporter synthesis pathway in the serum. The supplementation of VE also significantly increased the relative abundance of Succiniclasticum and decreased the relative abundance of Treponema, thus reducing the production of TVFA in the rumen. In conclusion, considering that the cows in this trial had high ketone levels (BHBA > 2.3 mmol/L), we found that VE could improve the rumen fermentation and blood metabolism by modulating the relative abundance of rumen microorganisms, thereby mitigating a range of adverse effects caused by SARA.

Differential carbon utilization enables co-existence of recently speciated Campylobacteraceae in the cow rumen epithelial microbiome

The activities of different microbes in the cow rumen have been shown to modulate the host’s ability to utilize plant biomass, while the host–rumen interface has received little attention. As datasets collected worldwide have pointed to Campylobacteraceae as particularly abundant members of the rumen epithelial microbiome, we targeted this group in a subset of seven cows with meta- and isolate genome analysis. We show that the dominant Campylobacteraceae lineage has recently speciated into two populations that were structured by genome-wide selective sweeps followed by population-specific gene import and recombination. These processes led to differences in gene expression and enzyme domain composition that correspond to the ability to utilize acetate, the main carbon source for the host, at the cost of inhibition by propionate. This trade-off in competitive ability further manifests itself in differential dynamics of the two populations in vivo. By exploring population-level adaptations that otherwise remain cryptic in culture-independent analyses, our results highlight how recent evolutionary dynamics can shape key functional roles in the rumen microbiome.

Features of rumen digestion of cows when an enzyme feed additive is included in the basic ration

Under the influence of microorganisms 95 % of sugars and starch, 70 % of fiber and 40–80 % of protein are broken down in the fore stomachs. Since the microfl ora of the rumen cannot always cope with the digestion of a large amount of coarse feed, enzyme drugs designed to increase productivity and preserve animal health come to their aid. The use of enzyme additives is relevant and allows us to prevent the action of anti-nutritional factors of feed, increase the activity of endogenous enzymes, thereby contributing to the increase in metabolic processes in the body and increase milk productivity. The purpose of this work was to study the features of microbial processes in the rumen of lactating cows when the multicomponent enzyme feed additive Kormomix® Enzyme was included. The use of the enzyme additive aff ected the physics and chemical parameters of the rumen fl uid. The pH concentration in all groups was the same. The volatile fatty acids in the 2nd and 3rd experimental groups were on the same level as the control. In the 1st experimental group, there was a decrease in volatile fatty acids by 1,15 mmol/100 ml compare with control group. The assimilation of ammonia in animals of the 2nd experimental group, which received 50 g /head /day of the enzyme additive, was higher in compare with the control group. The ratio of volatile fatty acids in the rumen fluid of cows of the experimental groups has changed towards the increase in the proportion of acetic acid. The composition of the bacterial community of the rumen improved with the inclusion of 50 g/head/day of the enzyme additive Kormomix® Enzyme. The number of cellulolytic microorganisms exceeded the control level by 6,45 abs.%. Thus, the use of the enzyme additive led to the intensification of microbiological processes in the rumen of cows.

К вопросу управления физиологическими процессами и формированием сбалансированного микробиома в рубце коров и овец путем введения в рацион кормовых факторов с определенными биологическими и функциональными свойствами

In physiological and production studies on intact and fistulated cows and sheep, the effect on enzymatic and microbiological processes in the rumen was studied when energy feed (EF) based on propylene glycol, glycerin and sugars was included in the standard diet of cows, separately and in combination with a complex of cellulolytic enzymes, consisting from cellulase, glucoaminolase, xylanase and β-gluconase (EF + CCE), and in the standard diet of sheep – micellate-Ca (M-Ca), microalgae Spirulina Platensis (Sp.Pl.), dihydroquercetin (DHQ) separately and in combination with organic iodine (DHQ+OJ). As a result of the use of these feed factors, there were changes in the indicators of digestion in the rumen of cows and sheep. In cows under the influence of EF in the rumen chyme, in relation to the control, cellulolytic activity increased by 75,4 %, and amylolytic activity decreased by 17,0 %, the content of VFA increased by 3,9 % and acetate by 2,2 %, and the content of propionate, butyrate and ammonia decreased by 5,6; 6,2 and 3,3 %, respectively. At the same time, there was an increase in the number of bacteria by 6,2 % and ciliates by 2,6 %, with a stable pH of 6,8. The inclusion of EF + CCE in the diet of cows did not affect the pH of the chyme, equal to 7,2; increased the content of VFAs by 3,6 % and reduced the content of ammonia by 3,8 % had almost no effect on the number of microorganisms, but changed its composition – the number of bacteria increased by 27,8 %, and the number of ciliates decreased by 23,5 %. In sheep, under the influence of M-Ca in the chyme, there was an increase in pH from 6,31 to 6,54, the content of VFAs by 6,6 %, amylolytic activity by 1,7 %, the number of microorganisms by 1,4 %, incl. ciliates by 0,4 %, bacteria by 2,2 % and the ammonia content decreased by 16,4 %. under the influence of Sp.Pl. there was an increase in pH from 6,31 to 6,91, amylolytic activity by 6,1 %, and a decrease in the content of VFAs by 18,2 %, ammonia by 3,4 %, the number of microorganisms by 5,4 %, incl. ciliates by 19,2 % and bacteria by 8,4 %; under the influence of DHQ, there was an increase in pH from 5,7 to 6,4, a decrease in the content of VFAs by 18,9 %, amylolytic activity by 7,3 %, ammonia by 24,1 %, an increase in the number of microorganisms by 2,2 % incl. h bacteria by 26,5 % with a decrease in the number of ciliates by 27,3 %; under the influence of DHQ + OJ, there was an increase in pH from 5,7 to 5,8, amylolytic activity by 1,1 %, ammonia content by 16.5 %, the number of microorganisms by 15,3 %, incl. ciliates by 56,1 % and bacteria by 8,7 %. All feed factors, with the exception of DHQ+OJ, shifted the redox potential of the chyme towards reduction, and under the action of DHQ+OJ, towards oxidation.

Similarity analysis of silage, rumen and milk microbiota in dairy cows

Similarity analysis of silage, rumen and milk microbiota in dairy cows

Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.

The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress. Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment). Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20). The yeast product contained 2.0 × 1010 CFU/g. Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035). Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030). In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05). Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates and protein. In fecal samples, LY did not affect the profile of VFAs (P > 0.05). Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, whereas Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis. In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows. These findings provide extended insight into the effects of LY in the rumen and hindgut of dairy cows exposed to heat stress.