Rotundus Rhizome Research Articles

A combinatorial approach to screen structurally diverse acetylcholinesterase inhibitory plant secondary metabolites targeting Alzheimer’s disease

Alzheimer's disease (AD) is a form of Dementia known to diminish the brain's function by perturbating its structural and functional components. Though cholinesterase inhibitors are widely used to treat AD, they are limited by numbers and side effects. Hence, present study aims to identify structurally diverse Acetylcholinesterase (AChE) inhibitory plant secondary metabolites (PSM) by employing high throughput screening and computational studies. AChE inhibitory activity was performed using 390 crude extracts from 63 plant parts belongs to 58 plants. The lowest IC50 value was recorded by acetone extract of Cyperus rotundus rhizome at 0.5 mg/ml, followed by methanol extract of Terminalia arjuna bark (0.95 mg/ml) and water extract Acacia catechu stem (0.95 mg/ml). A virtual library containing 487 PSM belongs to 18 plants found positive for AChE inhibition (IC50≤5 mg/ml) was prepared. Through ADMET analysis, 78 PSM fulfilling selected drug-likeness parameters were selected for further analysis. Molecular docking studies of selected PSM against AChE recorded a wide range of binding energy from −3.40 to −10.90 Kcal/mol. Further molecular dynamics simulation studies also recorded stabilized interactions of AChE-ligand complexes in the term of RMSD, RMSF, Rg, SASA, and hydrogen bond interaction. MMPBSA analysis revealed the binding energy of selected PSM ranging from −123.757 to −261.697 kJ/mol. Our study demonstrated the potential of 12 PSM (Sugiol, Margolone, 7-Hydroxy-3',4’-(Methylenedioxy) flavan, Beta-cyprone, Ethenone, Isomargolonone, Serpentine, Cryptolepine, Rotundone, Strictamin, Rotundenol and Nootkatone) as AChE inhibitors. Further in vitro and in vivo experimental evaluations with pure PSM could be beneficial for therapeutic uses.

Cyperus rotundus cyperaceae: a study of phytochemistry, total polyphenol content, flavonoid content, and antioxidant activity

Cyperus rotundus is a folk medicine with many benefits such as anti-inflammatory, anti-cancer, antioxidant, etc. It is widely used in many parts of the world. This study provided data on the phytochemical composition of Cyperus rotundus rhizome, including the active groups of alkaloids, flavonoids, phenols, tannins, and triterpenoids. The polyphenols content in the ethanolic and aqueous extracts of Cyperus rotundus were 26.89 ± 4.33 (mgGAE/g) and 51.84 ± 6.46 (mgGAE/g), respectively. While, the flavonoids content in the ethanolic and aqueous extracts of Cyperus rotundus were 78.03 ± 3.11 (mgQE/g) and 20.28 ± 2.25 (mgQE/g), respectively. According to the ABTS assay, the IC50 values of the aqueous and ethanolic extracts were 117.8 ± 19.85 (μg/ml) and 162.02 ± 11.43 (μg/ml), respectively. According to the DPPH assay, the IC50 values of Cyperus rotundus extracted by water and alcohol were 337.42 ± 22.84 (μg/ml) and 447.53 ± 33.8 (μg/ml), respectively. With the rich presence of secondary compounds, antioxidant activity confirmed in many studies, Cyperus rotundus is evaluated as having potential applications in many different fields such as pharmaceuticals, cosmetics, and functional foods.

Shelf-life assessment of bread containing Cyperus rotundus rhizome aqueous extract with antimicrobial compounds identified by 1H-NMR

This study evaluated the effects of Cyperus rotundus rhizomes aqueous extract on the shelf life of wheat bread. The aqueous extract strongly inhibited the growth of Aspergillus niger (78%), Penicillium spp. (89%) Bacillus subtilis (95%) and Bacillus cereus (90%). The C. rotundus extract had no significant (p≥0.05) effect on the moisture content (44.2–45.7) and water activity (0.86–0.87) of the wheat bread, significantly (p<0.05) increasing the phenolic content from 7.22 to 37.95 mg g−1 and decreasing the pH from 6.12 to 5.11. Fungal growth was observed on the treated bread after 11 days at 30 °C compared to 4 days for the control bread. However, the addition of the aqueous extract of C. rotundus affected the sensory attributes of the wheat bread but the bread was still considered acceptable by the trained panellists. In conclusion, the aqueous extract of C. rotundus rhizomes is a potential natural alternative to synthetic preservatives to extend the shelf life of wheat bread with high consumer acceptability.

Vasorelaxant Effect of Boesenbergia rotunda and Its Active Ingredients on an Isolated Coronary Artery.

Cardiovascular diseases are a major cause of death in developed countries. The regulation of vascular tone is a major approach to prevent and ameliorate vascular diseases. As part of our ongoing screening for cardioprotective natural compounds, we investigated the vasorelaxant effect of rhizomes from Boesenbergia rotunda (L.) Mansf. [Boesenbergia pandurata (Roxb.) Schltr.] used as a spice and herbal medicine in Asian countries. The methanol extract of B. rotunda rhizomes (BRE) exhibited significant vasorelaxation effects ex vivo at EC50 values of 13.4 ± 6.1 μg/mL and 40.9 ± 7.9 μg/mL, respectively, with and without endothelium in the porcine coronary artery ring. The intrinsic mechanism was evaluated by treating with specific inhibitors or activators that typically affect vascular reactivity. The results suggested that BRE induced relaxation in the coronary artery rings via an endothelium-dependent pathway involving NO-cGMP, and also via an endothelium-independent pathway involving the blockade of Ca2+ channels. Vasorelaxant principles in BRE were identified by subsequent chromatographic methods, which revealed that flavonoids regulate vasorelaxant activity in BRE. One of the flavonoids was a Diels-Alder type adduct, 4-hydroxypanduratin A, which showed the most potent vasorelaxant effect on porcine coronary artery with an EC50 of 17.8 ± 2.5 μM. Our results suggest that rhizomes of B. rotunda might be of interest as herbal medicine against cardiovascular diseases.

Test the Effectiveness of Infusion Formulation of Tamarind Fruit (Tamarindus indica) with Temu Kunci (Boesenbergia Rotunda) Rhizome against Head Lice Mortality (Pediculus Humanus Capitis)

Pediculosis capitis is an infectious disease caused by the parasite infestation Pediculosis humanus capitis. It is necessary to do alternative medicine by using natural ingredients as insecticides to kill head lice, one of which is tamarind fruit (Tamarindus indica) and temu Kunci rhizome (Boesenbergia rotunda) which have chemicals that work as insecticides. The form of the research is experimental in nature at the Laboratory of Clinical Parasitology, University of Muhammadiyah Sidoarjo. The sample used was head lice obtained as many as 480 tails from santriwati Pondok Pesantren Nurul Ikhlas Sidoarjo tested with tamarind fruit and rhizome of Temu Kunci obtained from the Probolinggo area, East Java. Samples were tested with 6 treatments, namely 100% tamarind fruit; 25% tamarind and 75% meeting lock; 50% tamarind and 50% meeting lock; 75% tamarind and 25% meeting lock; 100% key retrieval; negative control (aquades) and 4 repetitions. The data obtained for 24 hours were analyzed by using the One Way ANOVA statistical test and continued with the LSD test. One Way ANOVA test obtained a significance value of P= 0.000 (p&lt;0.005) and in the LSD further test, the most effective infusion solution was obtained at K4 (75% tamarind fruit and 25% rhizome of Temu Kunci). The conclusion is that there is an effectiveness of infusion formulation of tamarind fruit (Tamarindus indica) with temu lock rhizome (Boesenbergia rotunda) which can eradicate head lice (Pediculus humanus capitis).

Comparison of Cytotoxicity between Ethyl Acetate and Ethanol Extract of White Turmeric (Kaempferia rotunda) Rhizome Extract Against HeLa Cervical Cancer Cell Activity

Aim: The aim of this study is to compare between ethanol and ethyl acetate rhizome extract of K.rotunda against HeLa cervical cancer cell in vitro. Material and Methods: Methods used in this research are test the chemical compound of extracts using Thin Layer Chromatography (TLC) and phytochemical screening test, also cytotoxicity test using MTT assay. Result: Ethyl acetate extract contains flavonoid, alkaloid, tannin, and triterpenoid, while ethanol extract have flavonoid, triterpenoid, and alkaloid. In addition, ethanol extract has strong cytotoxic activity (IC50 = 16,939 μg/ml) while ethyl acetate extract has moderate cytotoxic activity (IC50 = 127,9 μg/ml). Each of extracts showed significant results (p ≤ 0,05) although when compared between concentrations there are several concentrations that are not significant and also small coefficient of determinant values caused by various confounding factors. Conclusion: The ethanol extract of K.rotunda rhizome extract has the higher cytotoxicity activity compared to ethyl acetate extract of K.rotunda rhizome extract against HeLa cervical cancer cell.

Boesenbergia rotunda extract inhibits Candida albicans biofilm formation by pinostrobin and pinocembrin

Ethnopharmacological relevanceBoesenbergia rotunda (L.) Mansf. (Zingiberaceae) is an indigenous plant of Southeast Asia. Based on ethnopharmacological use, the rhizome is recommended in the treatment of stomachache, leukoplakia, abscesses, and leukorrhea in Thailand primary health care system. Candida albicans often causes leukorrhea, and infection of many mucosal sites. Its infection leads to serious illness. Aim of the studyThis study aimed to investigate the effects of the ethanolic extract of the B. rotunda rhizome on C. albicans ATCC10231 in the stages of planktonic and biofilm formation and to explore the underlying mechanisms. Materials and methodsThe chemical composition of the extract was determined using ultra-performance liquid chromatography (UPLC). The planktonic growth of C. albicans was evaluated by the microdilution method, following EUCAST guidelines. For each stage of biofilm formation, the biofilm was assessed by the MTT assay. The biofilm structure was examined under a light microscope. The degree of cell surface hydrophobicity was measured. The mRNA levels of ALS1, ALS3, and ACT1 were determined by RT-qPCR. ResultsThe extract of B. rotunda consisted of 25% (w/w) pinostrobin and 12% (w/w) pinocembrin. All stages of C. albicans biofilm formation were significantly inhibited by the extract, whereas the planktonic growth did not change. Biofilm development greatly decreased due to the extract in a concentration-dependent manner, with an IC50 value of 17.7 μg/mL. Pinostrobin and pinocembrin demonstrated inhibitory effects during this stage. These results were in accordance with the microscopic evaluation. The filamentous form decreased with pinocembrin rather than pinostrobin. Moreover, the cell surface hydrophobicity was significantly decreased by 6.25 and 12.5 μg/mL of the extract and 100 μM of pinocembrin. The ALS3 mRNA level was noticeably decreased by 12.5 μg/mL of the extract, 100 μM of pinostrobin, and 100 μM of pinocembrin. The ACT1 mRNA level decreased significantly with pinocembrin. However, the ALS1 mRNA level was not altered following all treatments. ConclusionThe ethanolic extract of B. rotunda could inhibit biofilm formation of C. albicans, especially during the biofilm development stage, by means of reducing the cell surface hydrophobicity and suppressing the ALS3 mRNA expression. Pinocembrin had a stronger effect on ALS3 mRNA expression than pinostrobin. Only pinocembrin significantly decreased the ACT1 mRNA level.

Chemical Composition, Antiviral and Cytotoxic Activities of Essential Oil from Cyperus rotundus Growing in Egypt: Evidence from Chemometrics Analysis

The essential oil of Egyptian Cyperus rotundus rhizomes was obtained by hydrodistillation and analyzed by GC/MS. The oil is composed mainly of oxygenated sesquiterpenes. Thirty-seven compounds were identified including, humulene epoxide (38.43 %) and caryophyllene oxide (21.03 %) as major components. Correlations between different C. rotundus essential oils from Egypt and other regions were studied based on its chemical composition through principal components analysis and agglomerative hierarchical clustering. The oil was tested for its antiviral activity against hepatitis A, herpes simplex type 1, and coxsackie viruses at non-cytotoxic concentration. The oil was also evaluated for its cytotoxicity on hepatic, breast, and prostate carcinomas. It showed a very weak activity against tested viruses with percent protection 7.98, 14.21 and 8.79 %, respectively at a maximum non-toxic concentration of 125 μg/mL and it exhibited moderate cytotoxicity against hepatic and breast cancer cell lines with IC50 values of 204.1±1.25 and 170.8±0.567 μg/mL, respectively. The oil did not exhibit any cytotoxicity against the prostate cancer cell line.

Public Health Approach of Ayurveda and Yoga for COVID-19 Prophylaxis.

Public Health Approach of Ayurveda and Yoga for COVID-19 Prophylaxis.

&lt;i&gt;In vitro&lt;/i&gt; cytotoxic and antimicrobial effects of a novel peroxysesquiterpene glucoside from the rhizomes of &lt;i&gt;Cyperus rotundus&lt;/i&gt; L (Cyperaceae)

Purpose: To study the antimicrobial and cytotoxic potential of a novel 3,9-peroxsesquiterpene-15-Oglucoside from Cyperus rotundus rhizomes, against HeLa cell line and selected strains of microorganisms.&#x0D; Methods: The rhizomes were macerated with methanol and fractionated with n-hexane, chloroform, ethyl acetate and butanol. High-performance liquid chromatography (HPLC) was performed together with chemical analysis of the fractions. The 3,9-peroxysesquiterpene-15-O-glucoside was purified through column chromatography of the ethyl acetate fraction, and its purity was determined via reversephase HPLC. Structural elucidation was done with Infrared (IR), proton-nuclear magnetic resonance (HNMR), carbon-13 nuclear magnetic resonance (13C NMR), and mass spectrometry (MS) spectroscopic analyses.&#x0D; Results: The isolated compound exhibited bactericidal and fungicidal activities against S. aureus and C. albicans at concentration, respectively, in the range of 32 – 100 μg/mL, while MTT assay results showed the cytotoxicity of the compound against eukaryotic (HeLa) cell line (IC50, 88.32 μg/mL).&#x0D; Conclusion: The isolated metabolite from the methanol extract of C. rotundus rhizome exhibits bactericidal, fungicidal, and cytotoxic potential. However, further studies are required to ascertain its suitability for use as a therapeutic agent.&#x0D; Keywords: Terpenoids, Column chromatography, Spectroscopy, Antimicrobial, Cytotoxicity, Cyperus rotundus

Preclinical study of the topical anti-inflammatory activity of Cyperus rotundus L. extract (Cyperaceae) in models of skin inflammation

Ethnopharmacological relevanceCyperus rotundus L. (Cyperaceae) is considered one of the most widely distributed plant species in the world, especially in tropical and subtropical regions. In addition, it is commonly used in India, China and Japan in traditional medicine to treat different diseases, including dermatitis and other skin disorders. Aim of the studyTo investigate the topical anti-inflammatory activity of C. rotundus rhizome ethanolic extract in models of acute and chronic dermatitis. Materials and methodsPhytochemical analysis was carried out using High-performance liquid chromatography-ultraviolet detection (HPLC/UV) to determine the presence of quercetin and chlorogenic acid in C. rotundus extract. Topical anti-inflammmatory effects of C. rotundus extract were evaluated on arachidonic acid (AA) and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice. Skin biopsies were collected and submitted to histological and enzymatic analysis to evaluate the C. rotundus effect in leukocyte migration into inflamed tissue. Antiproliferative activity of C. rotundus was confirmed by PCNA immunostained cell analysis. Systemic and possible adverse effects of topical treatment with C. rotundus were evaluated by the skin atrophy and same organ weights. In addition, the glucocorticoid receptor (GR) antagonist mifepristone was used to investigate possible GR-mediated mechanisms of action. ResultsThe phytochemical analysis show that C. rotundus ethanol extract contains 45 μg/g of chlorogenic acid. Topical treatment with C. rotundus extract reduced ear edema and cellular infiltrate in acute and chronic skin inflammation models. Moreover, mice topically treated with C. rotundus exhibited decrease in TPA-induced keratinocyte hyperproliferation. Relevantly, topical treatment with C. rotundus did not caused skin atrophy or changes in lymphoid organ weight. The anti-inflammatory effect of C. rotundus was not influenced by the GR antagonist. ConclusionThe results here demonstrate for the first time the topical anti-inflammatory and antiproliferative efficacy of C. rotundus extract, suggesting that the extract could be a potential new therapeutic tool for the treatment of inflammatory skin disorders.

Determination of Quercetin and Flavonol Synthase in Boesenbergia rotunda Rhizome.

Flavonols in plants are catalyzed by flavonol synthase (FLS) enzyme. FLS was reported expressed in flowers and fruits, i.e., Dianthus caryophyllus L. (Caryophyllaceae), Petunia hybrida Hort. (Solanaceae), Arabidopsis thaliana L. (Brassicaceae), Citrus unshiu Marc. (Rutaceae). However, none reported about FLS in medicinal plants, particularly those which possess anti-inflammatory activity. This study was aimed to extract and identify FLS in the rhizome of Boesenbergia rotunda (Zingiberaceae) and to determine quercetin in the ethanol extract of the rhizome. The protein extraction of the rhizome was carried out by employing Laing and Christeller's (2004) and Wang's (2014) methods. The extracted-proteins were separated by using SDS-PAGE, followed by the measurement of FLS intensity by using Gel Analyzer. The FLS-1 of recombinant A. thaliana was employed as the standard. The determination of quercetin in the rhizome was carried out using LC-MS. The FLS occurred as a thick band at 38 kDa with intensity 116-158. The LC chromatogram of the extract indicated a small peak at 7.94 min similar to that of quercetin standard. The MS spectra at 7.94 min indicated that quercetin is present in the B. rotunda rhizome (m/z = 303.0549). The concentration of quercetin in the extract is 0.022% w/v. The FLS, an enzyme which plays an important role in producing quercetin, was detected in B. rotunda rhizome planted in Indonesia. As a consequence, quercetin in a small amount, was also quantified in the rhizome of this plant. This report will add a scientific insight of B. rotunda for biological sciences.

Effect of Chitosan, Chitosan Nanoparticle, Anacyclus pyrethrum and Cyperus rotundus in Combating Plasmid Mediated Resistance in Periodontitis

Background: Chitosan, chitosan nanoparticle, ethanolic extracts of Anacyclus pyrethrum root and Cyperus rotundus rhizome were evaluated against plasmid mediated multidrug resistance of Enterococcus faecalis, Staphylococcus aureus and Bacillus sp. isolated from unstimulated saliva of chronic periodontitis. The main aim of the current study centres the reduction of antibiotic consumption and the development of natural compounds to combat multidrug resistance. Methods: Identification of bacteria, antimicrobial susceptibility, plasmid stability and plasmid curing was carried out for the characterization of resistance plasmids. Results: E. faecalis showed 89% of sensitivity to chitosan nanoparticle, chitosan 81%, C. Rotundus 69% and A. pyrethrum 62% for S. aureus, with the MIC &gt;100 µg/ml. In comparison with the antimicrobials tested, maximum resistance to tetracycline (89%) for E. faecalis, is followed by ampicillin (87%) and tetracycline (81%) for S. aureus. Meanwhile, chloramphenicol and tetracycline (80%) for Bacillus sp. The plasmid stability for E. faecalis (20%), S. aureus (&lt;4%) and Bacillus sp. (16%). The order of stability corresponding to the broth media is LB ˃ BHI ˃ nutrient for E. faecalis, S. aureus but similar in LB and nutrient broth for bacillus sp. The maximum plasmid curing efficiency of chitosan for S. aureus (76%), Chitosan nanoparticle for E. faecalis (88%), A. pyrethrum for S. aureus (73%), C. rotundus for E. faecalis (87%). The order of plasmid curing efficiency is chitosan nanoparticle&gt;C. rotundus&gt;chitosan&gt;A. pyrethrum. Conclusion: Chitosan, Chitosan nanoparticle, C. rotundus, and A. pyrethrum offered a greater potential in eliminating plasmid mediated resistance acquired by periodontal pathogens, thus addressing this crisis in dentistry.

Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats.

Materials and Methods Forty-eight male Wistar rats were divided into anti-inflammatory mechanism study (n = 18) and acute toxicity study (n = 30). The anti-inflammatory mechanism study employed six groups (n = 3), e.g., the normal control, negative control, positive control (quercetin 20 mg/kg BW), and three doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW). All groups (except the normal control) were inflammatory-induced i.p. using 0.1 mL of 1% of acetic acid. The expression of Akt and NF-kappaB p65 in the stomach and intestine of the rats was examined using Western blot analysis. The acute toxicity study (21 days) was conducted by following the Regulation of Indonesia National Agency of Drug and Food Control No. 7/2014 about In Vivo Nonclinical Toxicity Study using 5 doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW; 2000 mg/kg BW; 4000 mg/kg BW). Results BREE reduces the infiltration of inflammatory cells in both the stomach and the intestine of acetic acid-induced rats. BREE also alters the expression of Akt and NF-kappaB p65 in the rat's stomach and intestine (p=0.005). The acute toxicity study reveals no lethal effects and behavioral signs of toxicity at all tested doses, which indicates that the LD50 is greater than 4000 mg/kg BW. Conclusion Taken together, BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats. This plant could be further explored for its potential as plant-based antistomach ulceration.

Therapeutic effects of Cyperus rotundus rhizome extract on memory impairment, neurogenesis and mitochondria in beta-amyloid rat model of Alzheimer's disease.

Alzheimer's disease (AD) is a progressive neurodegenerative disturbance leading to memory deficit, cognitive decline, and behavioral disturbance. Deposition of Amyloid beta plaques, neurofibrillary tangle and mitochondrial impairment are common neuropathological signs in AD. In this study, the effect of standardized Cyperus rotundus(C. rotundus) extract in three different doses of 250, 500, and 750mg/kg on memory, neurogenesis and mitochondrial mass in the beta amyloid rat model was assessed. For this purpose, 42 male Wistar rats were randomly divided into six groups (n = 7) to evaluate baseline training performance in Morris water maze test. Amyloid beta (Aβ) was injected in animal hippocampal CA1 bilaterally in four groups. After 21days, a decrease was observed in spending time in target quadrant in the first probe trial in Aβ injected groups. Following that, 250, 500, and 750mg/kg of C. rotundus extracts were administered to three out of four groups for a period of one month. BrdU (Bromodeoxyuridine) was intraperitoneally injected in all groups on the last 7days of treatment. Then, 28days after the last BrdU injection, the second probe trial was run, and rats were sacrificed. The neurogenesis and mitochondrial distribution were detected in hippocampus, by immunohistochemical staining. At last, it was observed that C. rotundus, almost recovered memory impairment, in addition to increasing in mitochondrial mass in CA1 and neurogenesis in dentate gyruse in the beta-amyloid rat model of Alzheimer's disease.

Synergistic Toxicity of Plant Essential Oils Combined with Pyrethroid Insecticides against Blow Flies and the House Fly.

Blow flies (Diptera: Calliphoridae) and the house fly (Diptera: Muscidae) are filth flies of medical importance, and control of their population is needed. As insecticide applications have resulted in fly resistance, and the exploration of plant essential oils (EOs) has increased against filth flies, this study assessed the combination of EOs with pyrethoids to enhance toxic efficacy. The EOs of five effective plants were screened initially against the house fly (Musca domestica L.). Their chemical constituent was performed using gas chromatography-mass spectrometry (GC-MS) analysis. The main components of Boesenbergia rotunda (Zingiberaceae) rhizome, Curcuma longa (Zingiberaceae) rhizome, Citrus hystrix (Rutaceae) fruit peel, Ocimum gratissimum (Lamiaceae) seed, and Zanthoxylum limonella (Rutaceae) fruit were δ-3-caren (35.25%), β-turmerone (51.68%), β-pinene (26.56%), p-cumic aldehyde (58.21%), and dipentene (60.22%), respectively. The screening test revealed that the three most effective plant EOs were from B. rotunda, C. longa and O. gratissimum, which were selected for the combination with two pyrethroid insecticides (permethrin and deltamethrin), in order to enhance their synergistic efficacy against the blow flies, Chrysomya megacephala Fabricius, Chrysomya rufifacies Macquart, and Lucilia cuprina Wiedemann, and the house fly. Synergistic action was presented in almost all of the flies tested with permenthrin/deltamethrin/EOs mixtures. It was interesting that the combination of deltamethrin with three EOs showed a synergistic effect on all of the tested flies. However, an antagonistic effect was observed in C. megacephala and M. domestica treated with permethrin-B. rotunda and C. megacephala treated with permethrin-O. gratissimum. The LD50 of insecticides decreased when combined with plant EOs. This alternative strategy will be helpful in developing a formula for effective fly control management.

The cytotoxic activity of local Cyperus rotundus phenolic extract on human breast cancer cell lines

Background: Cyperus rotundus L. is a herbal plant used worldwide to treat different diseases in traditional medicine, includingmalignancy.Methods: Purified phenolic compounds of C. rotundus rhizomes had been extracted, and GC-MS analysis of phenolic extract wasperformed and conducted according to the National Institute of Standard and Technology. The cytotoxic activity of C. rotundusphenolic extract investigated on various cell lines of human breast cancer (MCF-7, AMJ13, CAL-51) and normal cell line HBL-100 using MTT assay to evaluate IC50. Acridine orange-propidium iodide (AO-PI) stains have been used to investigated theapoptosis effects.Results: results of GC-MS analysis revealed that the extraction of C. rotundus rhizomes contained six phenolic compounds.C. rotundus phenolic extract displayed a significant cytotoxic efficacy against cancer cells MCF-7, CAL -51, and AMJ-13 with(IC50) of (135.3± 2.887, 218.6± 6.009, and 148.4± 4.619) μg /ml, respectively, but it has a negligible effect on HBL-100 at theseconcentrations with IC50 of 329.6± 5.196 μg/ml.Conclusion: The presence of anticancer activity in Cyperus rotundus phenolic extract suggested a further research in screeningthese phytochemicals and investigating their cytotoxic activity against various types of cancer cell lines.

Protective role of polyphenolic compounds extracted from cyperus rotundus rhizomes and taurine on troponin-I and some oxidant/antioxidants parameters of female rats treated with isoproterenol – induced myocardial infraction.

The present study was designed to investigate the effects of polyphenolic compounds extracted from cyperus rotundus rhizomes and taurine on isoproterenol induced myocardial infraction in female rats. Sixty of female rats (Rattus norvegicus) were used in the present study. Animals were divided into 10 groups ( 6 for each group). MI was induced in rats with ISO (85 mg/kg) twice at an interval of 24 hrs. ISO produced significant alterations in the troponin-I, serum MDA, tissue MDA, Cp, Alb, uric acid, serum GSH and tissue GSH. The effect of polyphenolic compounds from cyperus rotundus rhizomes oral treatment for 21 days at two doses (15 mg and 30 mg/kg, body weight) and taurine at two doses ( 100 mg and 200 mg/kg, body weight) was evaluated against ISO- induced cardiac necrosis. The results showed a significant increase in the level of serum troponin-I, serum MDA, tissue MDA, Cp and uric acid in ISO- treated animals as compared with the control animals. While, the results showed asignificant decrease in the level of serum Alb, serum GSH and tissue GSH in ISO- treated animals as compared with the control animals. Polyphenolic compounds from cyperus rotundus rhizomes and taurine showed a significant cardioprotective activity by lowering the levels of serum troponin-I, serum MDA, tissue MDA, Cp and uric acid and elevate the levels of Alb, serum GSH and tissue GSH. This study concluded that the polyphenolic compounds from cyperus rotundus rhizomes and taurine possess cardioprotective activity against Isoproterenol induced myocardial infraction in rats.

Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.

A new dinorcassane diterpenoid, seikphoochinal A (1), and four known compounds, pinostrobin (2), 4',7-dimethylkaempferol (3), and galanals A (4) and B (5), were isolated from the chloroform-soluble crude extract of wild type Boesenbergia rotunda rhizomes collected in Lower Myanmar. The chemical structures of these compounds were identified, using a combination of spectroscopic methods. The presence of the diterpenoids 1, 4, and 5 demonstrated the structural diversity of wild type B. rotunda. Among the isolates, compounds 4 and 5 exhibited significant antiproliferative activities against a small panel of human cancer cell lines, including lung (LK-2, A549), stomach (ECC4), breast (MCF7), cervix (HeLa), and prostate (DU145).

The in vitro and in vivo anti-hepatotoxic, anti-hepatitis B virus and hepatic CYP450 modulating potential of Cyperus rotundus

In the present study we investigated the hepatotoprotective, hepatitis B virus (HBV) inhibitory and hepatic CYP450 enzyme (CYP3A4) modulatory potential of Cyperus rotundus rhizome fractions. The crude ethanol-extract, including different organic and aqueous fractions were tested for in vitro cytoprotection on HepG2 cells (MTT assay), followed by in vivo evaluation in Wistar rats (serum biochemistry and lipid profile). The in vitro anti-HBV activity was tested on HepG2.2.15 cells (HBsAg and HBeAg Elisa). Of these, the n-butanol and aqueous fractions showed the most promising, dose-dependent hepatoprotection in DCFH-injured HepG2 cells. Further, in CCl4-injured rats, oral administration of C. rotundus (100 and 200 mg/kg·bw/day) significantly normalized serum markers of healthy liver function (SGOT, SGPT, GGT, ALP and bilirubin) and lipid profile (cholesterol, HDL, LDL, VLDL, TG and MDA), including tissue NP-SH and TP levels. Compared to other fractions, the ethyl acetate, n-butanol and aqueous fractions exhibited the best inhibitory effects on viral HBsAg and HBeAg secretions in dose- and time-dependent manner. In addition, reporter gene assay (Dual-luciferase) of transfected HepG2 cells showed mild activation of nuclear PXR-mediated CYP3A4 gene by the three active fractions. Taken together, C. rotundus showed very promising hepatoprotective and anti-HBV potential in experimental settings. In addition, this is the first report on modulation of CYP3A4 by C. rotundus that suggests its safe consumption in relation to drug metabolism and efficacy. Our data could therefore, provide the basis for the ethnobotanical medicinal use of C. rotundus in metabolic liver disorder and hepatitis B patients.