Abstract Soil pH is known to affect most soil biological processes, including denitrification. There are conflicting results as to the effect of pH on the denitrification process and the ability of denitrifiers to adapt to acid soil conditions. The denitrification potential and dynamics of the reduction enzymes involved were investigated for four soils of different pH values from the Park Grass Experiment at Rothamsted Experimental Station. Soils were incubated aerobically or anaerobically after being amended with 500 mg NO3--N kg-1. Anaerobic treatments were with or without C2H2 or with or without chloramphenicol (known to inhibit protein synthesis). All samples were incubated for 48 h, while CO2 and N2O production and NO3-, NO2- and NH4+ concentrations were monitored. CO2 and N2O production decreased with decreasing pH under both aerobic and anaerobic conditions. The addition of chloramphenicol under anaerobic conditions appeared to have no effect on CO2 production, but decreased N2O production in soils of pHCaCl2 greater than or equal to 5, but not in soils of pHCaCl2 less than or equal to 4, where N2O production was generally slow. Investigations of the enzyme dynamics involved in denitrification using the DETRAN model, suggested that the initial concentrations of N reductase enzymes were small in all soils and the biomass of Park Grass was not adapted to anaerobiosis. Data also suggested that soils of pHCaCl2 greater than or equal to 4.9, NO2- could accumulate to high concentrations (15-30 mg N kg-1) during anaerobic periods of 6-36 h. In the aerobic incubations nitrification was observed in all soils even at pHCaCl2 less than or equal to 3.9. Antecedent water regime of the soil appeared to be important in controlling denitrification activity and the dynamics of the reduction enzymes involved in the process. There were significant effects of pH on the number of microorganisms, the time to de novo enzyme synthesis and on the kinetics of the reduction enzymes involved in denitrification.
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