Root Structure Research Articles

Essential magnetosome proteins MamI and MamL from magnetotactic bacteria interact in mammalian cells

To detect cellular activities deep within the body using magnetic resonance platforms, magnetosomes are the ideal model of genetically-encoded nanoparticles. These membrane-bound iron biominerals produced by magnetotactic bacteria are highly regulated by approximately 30 genes; however, the number of magnetosome genes that are essential and/or constitute the root structure upon which biominerals form is largely undefined. To examine the possibility that key magnetosome genes may interact in a foreign environment, we expressed mamI and mamL as fluorescent fusion proteins in mammalian cells. Localization and potential protein-protein interaction(s) were investigated using confocal microscopy and fluorescence correlation spectroscopy (FCS). Enhanced green fluorescent protein (EGFP)-MamI and the red fluorescent Tomato-MamL displayed distinct intracellular localization, with net-like and punctate fluorescence, respectively. Remarkably, co-expression revealed co-localization of both fluorescent fusion proteins in the same punctate pattern. An interaction between MamI and MamL was confirmed by co-immunoprecipitation. In addition, changes in EGFP-MamI distribution were accompanied by acquisition of intracellular mobility which all Tomato-MamL structures displayed. Analysis of extracts from these cells by FCS was consistent with an interaction between fluorescent fusion proteins, including an increase in particle radius. Co-localization and interaction of MamI and MamL demonstrate that select magnetosome proteins may associate in mammalian cells.

Carbon Sequestration in a Changing Climate: Management Techniques and Strategic Solutions

Carbon sequestration in agriculture plays a critical role in mitigating climate change by capturing and storing atmospheric carbon dioxide (CO2) in soil and biomass. This process involves various strategies and management practices aimed at enhancing soil organic carbon (SOC) levels while maintaining or improving agricultural productivity. Key strategies include no-tillage or reduced tillage, which minimizes soil disturbance and promotes residue retention, thus slowing down decomposition and enhancing carbon storage. Crop rotation and the use of cover crops further contribute to carbon sequestration by diversifying root structures and improving soil health. Organic farming practices, such as applying farmyard manure and compost, provide essential organic matter that enriches soil quality and increases carbon retention. Additionally, the incorporation of deep-rooted plants and biochar enhances carbon storage in deeper soil layers, making them more resilient to climate variability. The integration of these practices not only sequesters carbon but also improves soil fertility, water retention, and overall ecosystem health. Effective management of carbon sequestration in agricultural systems requires a holistic approach that considers local conditions, crop diversity, and sustainable land use practices. By adopting these strategies, agriculture can become a significant carbon sink, contributing to global efforts to reduce atmospheric CO2 concentrations while ensuring food security and environmental sustainability.

Rogue wave patterns associated with Adler–Moser polynomials featuring multiple roots in the nonlinear Schrödinger equation

AbstractIn this work, we analyze the asymptotic behaviors of high‐order rogue wave solutions with multiple large parameters and discover novel rogue wave patterns, including modified claw‐like, one triple root (OTR)‐type, modified OTR‐type, two triple roots (TTR)‐type, semimodified TTR‐type, and modified TTR‐type patterns. A correlation is established between these rogue wave patterns and the root structures of the Adler–Moser polynomials with multiple roots. At the positions in the ‐plane corresponding to simple roots of the Adler–Moser polynomials, these high‐order rogue wave patterns asymptotically approach first‐order rogue waves. At the positions in the ‐plane corresponding to multiple roots of the Adler–Moser polynomials, these rogue wave patterns asymptotically tend toward lower‐order fundamental rogue waves, dispersed first‐order rogue waves, or mixed structures of these rogue waves. These structures are related to the root structures of special Adler–Moser polynomials with new free parameters, such as the Yablonskii–Vorob'ev polynomial hierarchy, among others. Notably, the positions of the fundamental lower‐order rogue waves or mixed structures in these rogue wave patterns can be controlled freely under specific conditions.

Evaluating Burn Severity and Post-Fire Woody Vegetation Regrowth in the Kalahari Using UAV Imagery and Random Forest Algorithms

Accurate burn severity mapping is essential for understanding the impacts of wildfires on vegetation dynamics in arid savannas. The frequent wildfires in these biomes often cause topkill, where the vegetation experiences above-ground combustion but the below-ground root structures survive, allowing for subsequent regrowth post-burn. Investigating post-fire regrowth is crucial for maintaining ecological balance, elucidating fire regimes, and enhancing the knowledge base of land managers regarding vegetation response. This study examined the relationship between bush burn severity and woody vegetation post-burn coppicing/regeneration events in the Kalahari Desert of Botswana. Utilizing UAV-derived RGB imagery combined with a Random Forest (RF) classification algorithm, we aimed to enhance the precision of burn severity mapping at a fine spatial resolution. Our research focused on a 1 km2 plot within the Modisa Wildlife Reserve, extensively burnt by the Kgalagadi Transfrontier Fire of 2021. The UAV imagery, captured at various intervals post-burn, provided detailed orthomosaics and canopy height models, facilitating precise land cover classification and burn severity assessment. The RF model achieved an overall accuracy of 79.71% and effectively identified key burn severity indicators, including green vegetation, charred grass, and ash deposits. Our analysis revealed a >50% probability of woody vegetation regrowth in high-severity burn areas six months post-burn, highlighting the resilience of these ecosystems. This study demonstrates the efficacy of low-cost UAV photogrammetry for fine-scale burn severity assessment and provides valuable insights into post-fire vegetation recovery, thereby aiding land management and conservation efforts in savannas.

Three-dimensional numerical modeling of soil-roots system based on X-ray computed tomography: Hydraulic effects study

Vegetation roots enhance soil stability by influencing saturation and pore structure, playing a pivotal role in stabilizing slopes, reducing erosion, and enhancing soil structure. However, current research on the hydraulic effects of roots on soil remains relatively limited. The micro-mechanisms of vegetation's impact on soil and the macro-level connections are not yet fully understood, which poses a challenge to the modeling of root-soil system. This study develops a three-dimensional (3D) finite element model of root-soil composites based on root computed tomography (CT) images and experimental results. Four different groups are modeled, including the rootless group, and those with Festuca arundinacea (FA) roots at various growth stages. The simulation results show that the saturation in the shallow layers significantly decreases in root-soil composite groups, and the rhizosphere water content is lower than that away from the roots, resulting in a net water flux toward the roots. The influence range of roots on suction is gradually amplified with increasing root growth process and root water uptake time. Higher levels of root development result in a stronger overall water uptake effect, leading to a more pronounced decrease in saturation. Closer proximity to the surface roots results in a more rapid increase in soil suction. Compared with one-dimensional root water uptake models, this model considers the effects of spatial heterogeneity of root structures on soil, which provides a comprehensive modeling basis for studying the effect of root system on soil.

An open-access lumbosacral spine MRI dataset with enhanced spinal nerve root structure resolution

Spinal cord injury (SCI) profoundly affects an individual’s ability to move. Fortunately, recent advancements in neuromodulation, particularly the spatio-temporal epidural electrical stimulation (EES) targeting the spinal nerve roots, promoted rapid rehabilitation of SCI patients. Such neuromodulation techniques require precise anatomical modelling of spinal cord. However, the lack of spine imaging datasets, especially high-quality magnetic resonance imaging (MRI) datasets highlighting nerve roots, hinders the translation of EES into medical practice. To address this problem, we introduce an open-access lumbosacral spine MRI dataset acquired in 14 healthy adults, using constructive interference in steady state (CISS) sequence, double echo steady state (DESS) sequence, and T2-weight turbo spin echo (T2-TSE) sequence, with enhanced nerve root resolution. The dataset also includes the corresponding anatomical annotations of nerve roots and the final reconstructed 3D spinal cord models. The quality of our dataset is assessed using image quality metrics implemented in MRI quality control tool (MRIQC). Our dataset provides a valuable platform to promote a wide range of spinal cord neuromodulation research and collaboration among neurorehabilitation engineers.

Comparative transcriptome and hormone analyses of roots in apple among three rootstocks with different rooting abilities

Background Root plays an important role in the growth and development of fruit trees; however, the molecular mechanisms behind the differences among rootstock varie-ties remain unclear. Methods This study examined the effects of different rootstocks on root structure and the endogenous hormone content of 1-year old apple seedlings in combinations of Tianhong 2 (T2)/Malus robusta (HT), T2/G935, and T2/Jizhen 2 (J2). Results The results showed that the T2/HT treatment had greater root length, surface area, volume, average diameter, tips and forks, followed by G935 and J2. In T2/HT leaves and roots, the indole-3-acetic acid (IAA) and gibberellins (GA3) levels were highest, and the abscisic acid (ABA) levels were the lowest. A root transcriptome analysis detected 10,064, 10,511, and 8,719 differentially expressed genes in T2/HT vs. T2/G935, T2/HT vs. T2/J2, and T2/J2 vs. T2/G935, respectively. The analysis of Gene Ontology (GO) terms indicated a significant enrichment in biological processes, cellular components, and molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that plant hormone signaling, MAPK signaling pathway–plant, and plant–pathogen interaction played important roles in differences in the rooting ability of different rootstocks. In addition, some key differential genes were associated with root growth and development and were involved in these metabolic pathways. This study is important for enriching theoretical studies of fruit tree roots.

The balance between alleviating copper damage and maintaining root function during root pruning with excessive copper.

Coating high concentrations of copper (Cu) on the inner wall of containers can efficiently inhibit root entanglement of container-grown seedlings. However, how the protective and defensive responses of roots maintain root structure and function during Cu-root pruning is still unclear. Here, Duranta erecta seedlings were planted in the containers coated with 40 (T1), 80 (T2), 100 (T3), 120 (T4), 140 (T5), and 160 (T6) g L-1 Cu(OH)2 with containers without Cu(OH)2 as the control. Although T5 and T6 produced the best inhibitory effect on root entanglement, root anatomy structure was damaged. T1 and T2 not only failed to completely control root circling, but also led to decreased root activity and stunted growth. Cu(OH)2 treatments significantly increased lignin concentration of roots with the highest values at T3 and T4. Compared with T3, seedlings at T4 had higher height, biomass, and root activity and no significant root entanglement. Excessive Cu accumulation in Cu(OH)2 treatments changed the absorption of other mineral nutrients and their allocation in the roots, stems, and leaves. Overall, Ca was decreased while Mg, Mn, Fe, and K were increased, especially K and Mn at T4 which is related to defense capacity. The results indicate that there is a Cu threshold to balance root entanglement control, defense capacity, and nutrient uptake function under excessive Cu for container-grown D. erecta seedlings.

Distribution of Non-Structural Carbohydrates and Root Structure of Plantago lanceolata L. under Different Defoliation Frequencies and Intensities.

Plantago lanceolata L. (plantain) increases herbage dry matter (DM) production and quality during warm and dry conditions due to its deep roots and drought tolerance and reduces nitrogen losses in grazing systems compared to traditional pastures. However, plantain density usually declines after the third growing season, mainly due to defoliation management. The effects of defoliation frequency and intensity on water-soluble carbohydrate (WSC) reserves and below-ground plant responses need further research to optimize grazing strategies for improved productivity and sustainability of grazing systems. Our study investigated the effects of defoliation frequencies (15, 25, and 35 cm of extended leaf length, ELL) and intensities (5 and 8 cm of residual heights) on morphological traits and WSC concentrations in plantain biomass under controlled environmental conditions. Defoliation frequency significantly influenced morphological and chemical characteristics and biomass distribution more than residual height. Less frequent defoliations promoted above-ground herbage DM production, reproductive stems, and root biomass. Root architecture showed adaptations in response to defoliation frequency, optimizing resource acquisition efficiency. Frequent defoliation reduced high molecular weight WSC concentrations in leaves, affecting regrowth capacity and DM mass. A defoliation frequency of 25 cm ELL (~15 days) balances herbage production and root development, promoting long-term pasture sustainability.

Mutation of MeMinD increased amyloplast size with a changed starch granule morphologenesis and structures in cassava storage roots

Amyloplasts are the sites of starch synthesis and accumulation. Little is known about amyloplast division and its effects on the size, structure, and physicochemical properties of starch granules. In this study, we created mutants of plastid division-related gene MeMinD by CRISPR/Cas9 technology, leading to the disruption of normal division of amyloplasts in cassava storage roots. The memind mutants exhibited significantly enlarged amyloplasts with an increased number of starch granules, and broader range of granule sizes. The loss of MeMinD function led to transcriptional reprogramming of gene expressions related to starch-synthesizing enzymes, affecting the fine structure of starch. Starch in memind mutant storage roots showed a significantly decreased proportion of shorter amylopectin chains and an increased proportion of medium and long chains, which ultimately led to a significant increase in apparent amylose content (AAC) in memind mutants compared to that in WT. The changes in starch granule size and structure resulted in a significant increase in onset temperature (To), peak temperature (Tp), and conclusion temperature (Tc) of the gelatinization process, extending the time to reach peak temperature. These data suggest that regulating amyloplast division affects starch accumulation in cassava, presenting an effective strategy for developing novel cassava starch.

Aortic root morphometry revisited-Clinical implications for aortic valve interventions.

The complex anatomy of the aortic root is of great importance for many surgical and transcatheter cardiac procedures. Therefore, the aim of this study was to provide a comprehensive morphological description of the nondiseased aortic root. We morphometrically examined 200 autopsied human adult hearts (22.0% females, 47.9 ± 17.7 years). A meticulous macroscopic analysis of aortic root anatomy was performed. The largest cross-section area of the aortic root was observed in coaptation center plane (653.9 ± 196.5 mm2), followed by tubular plane (427.7 ± 168.0 mm2) and basal ring (362.7 ± 159.1 mm2) (p < 0.001). The right coronary sinus was the largest (area: 234.3 ± 85.0 mm2), followed by noncoronary sinus (218.7 ± 74.8 mm2) and left coronary sinus (201.2 ± 78.08 mm2). The noncoronary sinus was the deepest, followed by right and left coronary sinus (16.4 ± 3.2 vs. 15.9 ± 3.1 vs. 14.9 ± 2.9 mm, p < 0.001). In 68.5% of hearts, the coaptation center was located near the aortic geometric center. The left coronary ostium was located 15.6 ± 3.8 mm above sinus bottom (within the sinus in 91.5% and above sinutubular junction in 8.5%), while for right coronary ostium, it was 16.2 ± 3.5 mm above (83.5% within sinus and 16.5% above). In general, males exhibited larger aortic valve dimensions than females. A multiple forward stepwise regression model showed that anthropometric variables might predict the size of coaptation center plane (age, sex, and heart weight; R2 = 31.8%), tubular plane (age and sex; R2 = 25.6%), and basal ring (age and sex; R2 = 16.9%). In conclusion, this study presents a comprehensive analysis of aortic-root morphometry and provides a platform for further research into the intricate interplay between structure and function of the aortic root.

The cleavage of WOX5 by the peptidase DA1 connects cytokinin signaling and root stem cell regulation.

The root system of a plant is essential for plant growth and development because it absorbs nutrients and water from the soil. The root stem cells are maintained and replenished by the quiescent center and are essential for the formation of the specific root structure. In Arabidopsis, the WUSCHEL-RELATED HOMEOBOX 5 (WOX5) plays a key role in regulating root stem cell fate. However, which factor can directly modulate the stability of WOX5 protein remains totally unknown. Here, we report that the peptidase DA1 (LARGE IN CHINESE) interacts with and cleaves WOX5, resulting in the destabilization of WOX5. Genetic analyses support that DA1 acts through WOX5 to regulate root stem cell function. We further demonstrate that cytokinin (CK) signaling induces the accumulation of DA1 protein, thereby decreasing the abundance of WOX5 protein in the root. Consistent with these results, the mutation in DA1 increases the layers of columella stem cells and influences CK-induced differentiation of columella stem cells. Thus, our results reveal a previously unrecognized mechanism by which the cleavage of WOX5 by DA1 connects CK signaling and root stem cell function in Arabidopsis.

Evaluation Of Lateral Root Resorption In Maxillary Teeth Among First Premolar Extraction Cases After Space Closure- A CBCT Study

Background: Orthodontic treatment usually involves usage of light continuous forces but when heavy forces are used it leads to apical root resorption. External apical root resorption refers to reduction in root structure involving the apices. This resorption is of transient inflammatory type. 2D images are commonly used for diagnostic purposes in orthodontics but it cannot detect root resorption on lingual or buccal surfaces thus cannot measure the volume of root loss. Cone beam computed tomography which can provide 3D images was introduced in late 1990s. Images provided by CBCT are more more accurate than panoramic and periapical radiographs . Therefore in this study , CBCT is used to evaluate lateral root resorption in maxillary teeth after after space closure among first premolar extraction cases . Materials and Methods: CBCTs of 20 patients fulfilling inclusion and exclusion criteria were taken before the start of orthodontic treatment and after space closure. Post treatment root width was substracted from the pre treatment root width at three levels from CEJ (3mm,6mm,9mm). The normality test (Shapiro-Wilk Test) was performed to analyze the data, and the results were expressed as frequency with percentage and mean with standard deviation. Difference between means were assessed using paired t test for before and after the procedure. All the statistical analyses were carried out at a 5% level of significance, and results with a P value &lt; 0.05 were considered statistically significant. Results: Considering all the three levels the least tooth resorption was observed in the case of central incisor (0.1750 ± 0.0444, p &lt;0.001) and highest in the canine tooth (5.900± 0.0000, p &lt;0.001) . Conclusion: When root resorption is detected, it is recommended to pause the treatment for about 6 months to allow for root repair. Intermittent forces, which provide rest periods between applications, are more tissuefriendly and can help reduce the risk of further resorption

Impact of cadmium on photosynthetic pigments, root structure, and nutrient concentrations in bean (Phaseolus vulgaris L.) plants

Impact of cadmium on photosynthetic pigments, root structure, and nutrient concentrations in bean (Phaseolus vulgaris L.) plants

A Spring Model for Pullout Behavior of Curved, Flexible Structures Embedded in Soil

ABSTRACTThe shape and flexibility of embedded structures, such as tree roots, piles, and anchors, have important impacts on the pullout behavior. However, the rate and manner of mobilization of soil resistances along such structures has not been rigorously explored across a wide range of shapes and structural properties. A spring model for computing compatible displacements of the structure and soil for curved, flexible structures is defined, validated against commonly used methods for computing pile pullout behavior, and then parametrically explored to demonstrate how resistances are mobilized along the length of such structures. The present model allows description of combined axial and transverse loading of these nonlinear structures. The simulation results for the case of normally consolidated clay show that the curvature of a structure causes the distribution of bearing resistance to extend further along the structure than for linear cases. The requirement of equilibrium of the structure produces a coupling between the mobilized bearing and tensile resistance in terms of rate of development and magnitude. Thus, the choices of structure shape impact the magnitude and distribution of mobilized resistance of embedded flexible structures. Implications for anchorage of tree root structures and principles of bioinspired design of anchorage systems are discussed.

Growth, Biochemical Traits, Antioxidant Enzymes, and Essential Oils of Four Aromatic and Medicinal Plants Cultivated in Phosphate-Mine Residues.

Revegetation emerges as a promising approach to alleviate the adverse impacts of mining residues. However, it is essential to evaluate the characteristics of these materials and select suitable plant species to ensure successful ecosystem restoration. This study aimed to investigate the effects of phosphate-mine residues (MR) on the growth, biochemical properties, and essential oil concentration of Rosmarinus officinalis L., Salvia Officinalis L., Lavandula dentata L., and Origanum majorana L. The results showed that R. officinalis L. appeared to be particularly well-suited to thriving in MR soil. Our finding also revealed that L. dentata L., O. majorana L., and S. officinalis L. grown in MR exhibited significantly lower growth performance (lower shoot length, smaller leaves, and altered root structure) and higher antioxidant activities, with an alterations of photosynthetic pigment composition. They showed a decrease in total chlorophylls when grown on MR (0.295, 0.453, and 0.562 mg g-1 FW, respectively) compared to the control (0.465, 0.807, and 0.808 mg g-1 FW, respectively); however, they produced higher essential oil content (1.8%, 3.06%, and 2.88%, respectively). The outcomes of this study could offer valuable insights for the advancement of revegetation technologies and the utilization of plant products derived from phosphate-mine residues.

On Seedling Morphoanatomy of Asteraceae Campestral Species: Some Adaptative and Taxonomic Considerations

Theoretical Framework: The vegetation of Parana State fields is composed mainly of woody grassy savanna, and one of the largest families in specific richness is Asteraceae. The campestral Asteraceae occurs in three microhabitats as dry fields (steppes “stricto sensu”), rocky outcrops (rupestrian vegetation refuges) and humid fields (higrophyllous steppes). Morphology and structure of Asteraceae seedlings can be useful for species recognition in the field at the juvenile stage, and to understand how seedlings establish themselves in different microhabitats. Objective: The aim of this paper was to analyze the seedling structure of 17 Asteraceae species, and to summarize some considerations on anatomical characters of the seedlings, which may be variations environmentally induced and they have some taxonomic or evolutionary value. Method: Cipselae of 17 species of Asteraceae were collected in the state parks of Paraná, Brazil, and placed to germinate in Petri plates. The seedling development occurred in greenhouse. Root, hypocotyl, cotyledons, epicotyl and eophylls were sectioned in rotation microtome, and analyzed in light microscope. Cotyledons and eophylls were investigated in a scanning electronic microscope. Results: Roots are diarch or tetrarch. Hypocotyl may have the root structure and partially or wholly as transition root-stem structure. Epicotyl shows the stem structure. Cotyledons and eophylls are dorsiventral. Trichome density and amphistomaty are xeromorphic characters that occur in the cotyledons and eophylls of the species. Conclusion: Some structural characters have diagnostic value for separation of investigated species, such as hypocotyl structure, root/stem transition type, epidermis, mesophyll tissues and midrib saliency of the cotyledons and eophylls, and presence of secretory ducts.

On Bethe equations of 2d conformal field theory

We study the higher spin algebras of two-dimensional conformal field theory from the perspective of quantum integrability. Starting from Maulik-Okounkov instanton R-matrix and applying the procedure of algebraic Bethe ansatz, we obtain infinite commuting families of Hamiltonians of quantum ILW hierarchy parametrized by the shape of the auxiliary torus. We calculate explicitly the first five of these Hamiltonians. Then, we numerically verify that their joint spectrum can be parametriezed by solutions of Litvinov’s Bethe ansatz equations and we conjecture a general formula for the joint spectrum of all ILW Hamiltonians, based on results of Feigin, Jimbo, Miwa and Mukhin.There are two interesting degeneration limits, the infinitely thick and the infinitely thin auxiliary torus. In one of these limits, the ILW hierarchy degenerates to Yangian or Benjamin-Ono hiearchy and the Bethe equations can be easily solved. The other limit is singular but we can nevertheless extract local Hamiltonians corresponding to quantum KdV or KP hierarchy. Litvinov’s Bethe equations in this local limit provide an alternative to Bethe ansatz equations of Bazhanov, Lukyanov and Zamolodchikov, but are more transparent, work at any rank and are manifestly symmetric under triality symmetry of W\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$ \\mathcal{W} $$\\end{document}1+∞. Finally, we illustrate analytic properties of the solutions of Bethe equations in minimal models, in particular for Lee-Yang CFT. The analytic structure of Bethe roots is very rich as it captures the representation theory of W\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$ \\mathcal{W} $$\\end{document}N minimal models.

Exploring Ground and Excited States Via Single Reference Coupled-Cluster Theory and Algebraic Geometry.

The exploration of the root structure of coupled cluster (CC) equations holds both foundational and practical significance for computational quantum chemistry. This study provides insight into the intricate root structures of these nonlinear equations at both the CCD and CCSD level of theory. We utilize computational techniques from algebraic geometry, specifically the monodromy and parametric homotopy continuation methods, to calculate the full solution set. We compare the computed CC roots against various established theoretical upper bounds, shedding light on the accuracy and efficiency of these bounds. We hereby focus on the dissociation processes of four-electron systems such as (H2)2 in both D2h and D∞h configurations, H4 symmetrically distorted on a circle, and lithium hydride. We moreover investigate the ability of single-reference CC solutions to approximate excited state energies. We find that multiple CC roots describe energies of excited states with high accuracy. Our investigations reveal that for systems like lithium hydride, CC not only provides high-accuracy approximations to several excited state energies but also to the states themselves.

Intercropping with Robinia pseudoacacia reduces soft rot incidence in konjac by modulating the root bacterial community.

Soft rot (Pectobacterium aroidearum and Dickeya) is a devastating soil-borne bacterial disease that threatens konjac production. Intercropping with false acacia has been shown to significantly reduce soft rot incidence in konjac by shifting the microbial community. However, how intercropping shapes the root bacterial community and affects soft rot incidence remains unclear. To address this, we investigated three konjac intercropping systems (false acacia, paulownia, and maize) to explore the relationships among intercropping, soft rot incidence, root bacterial community, soil enzyme activity, and soil properties. Konjac intercropped with false acacia exhibited the lowest soft rot incidence and the lowest abundance of pathogenic taxa. Soft rot incidence was negatively correlated with total soil nitrogen and potassium but positively correlated with total and available soil phosphorus. The bacterial community structure and function in konjac roots differed among intercropping types, mainly driven by available soil phosphorus. Beneficial microorganisms such as Bradyrhizobium and Variovorax were enriched under a false acacia intercropping system and were negatively correlated with soil-available phosphorus. Additionally, the stable bacterial community in healthy konjac roots under false acacia may make konjac less susceptible to pathogen invasion. The study showed that intercropping reduced the soft rot incidence by regulating the structure and stability of the konjac root bacterial community, and soil-available phosphorus was the main factor affecting the difference in the konjac root bacterial community, which provided a basis for the management of soil fertilization in konjac cultivation. © 2024 Society of Chemical Industry.