Root Resorption Of Teeth Research Articles

Morphological analysis of cell cannibalism: An auxiliary tool in the prediction of central giant cell granuloma clinical behavior

Central giant cell granuloma (CGCG) is a benign jaw lesion with variable clinical behavior. Cell cannibalism is a cellular process associated with aggressiveness and invasion in malignant neoplasms. Here, we morphologically investigated cell cannibalism as an auxiliary method to predict CGCG clinical behavior. Cell cannibalism was quantitatively evaluated in 19 cases of peripheral giant cell granuloma (PGCG), 38 cases of CGCG (non-aggressive and aggressive), and 19 cases of giant cell tumor of bone (GCT) stained with hematoxylin and eosin. T-test was performed to assess the differences between the variables analyzed (p ≤ 0.05). Cell cannibalism was identified in 21% of non-aggressive CGCGs and 68.4% of aggressive CGCGs. A significantly higher amount of cannibal multinucleated giant cells (CMGC) was observed in aggressive CGCG compared to PGCG and non-aggressive CGCG (p = 0.042; p = 0.044, respectively). There were no significant differences in the CMGC index between non-aggressive CGCG and PGCG (p = 0.858) and between aggressive CGCG and GCT (p = 0.069). CGGC cases that exhibited rapid growth and tooth displacement and/or root resorption had a higher amount of CMGC (p = 0.035; p = 0.041, respectively). Cell cannibalism can be identified in CGCG through routine anatomopathological examination. The quantification of CMGC can help to predict the clinical behavior of central giant cell granuloma.

Predictors of complications associated with unerupted maxillary canines: a cone beam computed tomography study.

To detect predictive factors for the occurrence of complications associated with unerupted maxillary canines (C). A total of 83 cone beam computed tomograms (CBCT), made from November 1, 2021 to October 31, 2022, have met the inclusion criteria, whereby 110 unerupted C were detected. Independent variables were: gender, age, and C position. Outcome variables were detected complications: external root resorption (ERR) and dilaceration of adjacent tooth, ERR and dilaceration of C, dentigerous cyst, canine ankylosis, and adjacent teeth malposition. Chi-square test and logistic regression analysis were used to examine the relationship between income variables and detected complications. Vertical position of unerupted C showed as predictor for adjacent tooth dilacerations and malposition. Chance for dilaceration increases 5.5-fold with C position at the middle third of lateral incisor (LI) root, while chance of malposition increases 23.4-fold with its supra-apical position to the LI root, in comparison to the C position occlusal to the LI cemento-enamel junction. Age is a predictive factor for dentigerous cysts, with decreasing the chance of their occurrence 1.5-fold with each additional year of age. Early evaluation of the vertical position and timely treatment of C are decisive steps in preventing complications.

LITTIP/Lgr6/HnRNPK complex regulates cementogenesis via Wnt signaling

Orthodontically induced tooth root resorption (OIRR) is a serious complication during orthodontic treatment. Stimulating cementum repair is the fundamental approach for the treatment of OIRR. Parathyroid hormone (PTH) might be a potential therapeutic agent for OIRR, but its effects still lack direct evidence, and the underlying mechanisms remain unclear. This study aims to explore the potential involvement of long noncoding RNAs (lncRNAs) in mediating the anabolic effects of intermittent PTH and contributing to cementum repair, as identifying lncRNA-disease associations can provide valuable insights for disease diagnosis and treatment. Here, we showed that intermittent PTH regulates cell proliferation and mineralization in immortalized murine cementoblast OCCM-30 via the regulation of the Wnt pathway. In vivo, daily administration of PTH is sufficient to accelerate root regeneration by locally inhibiting Wnt/β-catenin signaling. Through RNA microarray analysis, lncRNA LITTIP (LGR6 intergenic transcript under intermittent PTH) is identified as a key regulator of cementogenesis under intermittent PTH. Chromatin isolation by RNA purification (ChIRP) and RNA immunoprecipitation (RIP) assays revealed that LITTIP binds to mRNA of leucine-rich repeat-containing G-protein coupled receptor 6 (LGR6) and heterogeneous nuclear ribonucleoprotein K (HnRNPK) protein. Further co-transfection experiments confirmed that LITTIP plays a structural role in the formation of the LITTIP/Lgr6/HnRNPK complex. Moreover, LITTIP is able to promote the expression of LGR6 via the RNA-binding protein HnRNPK. Collectively, our results indicate that the intermittent PTH administration accelerates root regeneration via inhibiting Wnt pathway. The lncRNA LITTIP is identified to negatively regulate cementogenesis, which activates Wnt/β-catenin signaling via high expression of LGR6 promoted by HnRNPK.

Central odontogenic fibroma: A case report

The central odontogenic fibroma (COF) is a rare benign odontogenic mesenchymal tumour of jaw bone derived from the odontogenic ectomesenchyme. It is characterized by a fibrous mature stroma with variable number of strands or islands of inactive-looking odontogenic epithelium. The presented case is a 46 years old male complaining of asymptomatic mandibular bony swelling. Radiographically, the lesion is unilocular radiolucent, with root resorption and displacement of teeth. Histopathology showed dense fibrous connective tissue stroma, loosely arranged with spindle and stellate-shaped mesenchymal cells, inactive odontogenic epithelium island and osteoid tissue. In this case report, we also discuss about the types, pathogenesis, and recent concepts regarding COF.

Factors that may increase the risk of external apical root resorption during orthodontic treatment : Retrospective clinical investigation.

To determine the correlation between external apical root resorption and malocclusion-related variables in patients treated with fixed orthodontic appliances. In all, 103patients aged 12-15years and treated with edge-wise appliances either without extractions or with four premolar extractions were included in this retrospective cohort study. External apical root resorption was assessed in the pre- and posttreatment panoramic radiographs of these patients for incisors, canines, premolars, and first molars. Atotal of 2332 teeth were evaluated in 206 panoramic radiographs obtained from 103patients. The gender of the patients, duration of orthodontic treatments, presence of premolar extractions, Angle classification, overbite, overjet and amount of crowding were assessed. Wilcoxon signed-rank test, Mann-WhitneyUtest, and Kruskal-Wallis test were used for statistical analysis of the data. Statistically significant (p < 0.001) root resorption occurred in all examined teeth during orthodontic treatment. The degree of root resorption observed in the premolars was significantly greater in premolar extraction cases than in nonextraction cases. Statistically significant negative correlations were found between the degree of root resorption of the maxillary lateral teeth and between the maxillary first premolar teeth and the amount of maxillary crowding. The orthodontic treatment protocols and the amount of tooth movements achieved were shown to be dependent on the severity of dental malocclusions and they have an influence on the amount of external apical root resorption that occurs during the course of orthodontic treatment.

Assessment of changes in maxillary dentoalveolar complex following retraction and intrusion of maxillary anterior teeth in mixed Indian population

The biology of tooth movement implies to selective resorption and deposition of the alveolar bone during orthodontic tooth movement. The controversy about "tooth movement through bone" and "tooth movement with bone” is still not settled. The cortices of the alveolar bone are considered boundaries of the alveolar complex that limit the orthodontic tooth movement. The present study evaluated the change in these limits of maxillary dentoalveolar complex post-retraction and intrusion of maxillary anterior teeth.: CBCT data of 15 subjects was assessed to primarily measure the changes in thickness, area and volume of anterior maxillary alveolar bone following retraction &amp; intrusion. As a secondary objective, tooth length was also assessed to ascertain external apical root resorption, post-retraction and intrusion of maxillary anterior teeth. A statistically significant reduction in alveolar bone thickness, mean area and volume on the palatal side was evident and also reduction in tooth length of maxillary central and lateral incisor was observed post retraction and intrusion of maxillary anterior teeth (P-value&amp;#60;0.05). It is essential to evaluate the alveolar housing prior to considering retraction and intrusion of anterior teeth in order to plan appropriate biomechanics and treatment protocol which would minimize iatrogenic side effects and yield a stable treatment outcome.

CONSERVATIVE TREATMENT OF ADENOID AMELOBLASTOMA: A CASE REPORT

A 27-year-old female presented painful swelling in the face, on the right mandibular side, 4 months prior to her appointment. Intraoral examination made it possible to observe swelling in the lower mandibular molar vestibule on the right side. Panoramic radiography disclosed a well corticated unilocular radiolucent lesion involving lower mandibular molars on the right side, with root resorption of teeth #46 and #47. Needle aspiration was performed, and a negative result was observed. The total enucleation of the lesion was then performed. The pathological examination revealed a benign odontogenic neoplasm characterized by the presence of infiltrating odontogenic cells arranged in whorls, duct-like structures in a cribriform arrangement, with peripheral palisading ameloblast-like cells, surrounded by a fibrous vascular stroma, with the presence of a moderate inflammatory infiltrate. The histopathological diagnosis was adenoid ameloblastoma. After 8 months, clinical and radiographic follow-up was performed, and a complete bone formation was observed. A 27-year-old female presented painful swelling in the face, on the right mandibular side, 4 months prior to her appointment. Intraoral examination made it possible to observe swelling in the lower mandibular molar vestibule on the right side. Panoramic radiography disclosed a well corticated unilocular radiolucent lesion involving lower mandibular molars on the right side, with root resorption of teeth #46 and #47. Needle aspiration was performed, and a negative result was observed. The total enucleation of the lesion was then performed. The pathological examination revealed a benign odontogenic neoplasm characterized by the presence of infiltrating odontogenic cells arranged in whorls, duct-like structures in a cribriform arrangement, with peripheral palisading ameloblast-like cells, surrounded by a fibrous vascular stroma, with the presence of a moderate inflammatory infiltrate. The histopathological diagnosis was adenoid ameloblastoma. After 8 months, clinical and radiographic follow-up was performed, and a complete bone formation was observed.

CLINICAL PATHOLOGIC CONFERENCE CASE 1: A MULTILOCULAR RADIOLUCENCY OF THE RIGHT MANDIBLE

CLINICAL PATHOLOGIC CONFERENCE CASE 1: A MULTILOCULAR RADIOLUCENCY OF THE RIGHT MANDIBLE

Endodontic-orthodontic interactions: a review and treatment recommendations.

The literature is replete with articles describing the many and varied interactions between endodontic treatment and orthodontic tooth movement (OTM), often reporting conflicting views and findings, which creates confusion for clinicians. Original research and review articles have described aspects such as apical root resorption and potential pulpal complications of teeth related to OTM. Some interactions are of relatively minor clinical significance, whilst others may have adverse consequences. A history of dental trauma before or during OTM further complicates the interactions. This review re-assesses the historical literature on endodontic-orthodontic interactions in light of more recent research and presents guidelines for managing clinical situations involving both disciplines. © 2023 Australian Dental Association.

Third molar impaction prevalence and pattern: a panoramic radiography investigation

Objectives: The most commonly encountered impacted tooth is the third molar, and potential problems include oral cysts and tumors as well as caries, periapical lesions, periodontal disease, TMJ disorders, and root resorption of adjacent teeth. The aim of this investigation was to determine the third molar impaction prevalence and pattern.&#x0D; Materials and Methods: 530 panoramic radiographs of patients who visited Hasanuddin University Dental Hospital from January to December 2020 were collected. Data were recorded based on third molars region, eruption level, angulation, and impaction class from radiographs that met the criteria on Excel sheets and analyzed using SPSS 26. Chi square test was performed to assess relationship between impaction patterns with age and gender. The level of significance was fixed at p&lt;0.05.&#x0D; Results: 115 radiographs were included and analyzed (45.2% male and 54.8% female). Third molar impaction prevalence was higher in females, in the age group 21-30 years, with level A eruption and vertical angulation. Most #38 and #48 demonstrated class 2 impaction. Apart from angulation and impaction class on #38, there was no significant difference between impaction patterns by age and gender group (p&gt;0.05).&#x0D; Conclusion: Third molar impaction was more common in women aged 21-30 years with a dominant impaction pattern at level A with vertical angulation. In the mandible, class 2 of the Pell and Gregory classification are known to be dominant in third molar impaction.

Evaluation of a correlation between the severity of root resorption of adjacent teeth and level of difficulty of impacted canine – A cone-beam computed tomography study

Objectives: An interrelation between the level of difficulty of canine impaction based on its three-dimensional position and the degree of root resorption of the adjacent teeth improves the quality of orthodontic treatment planning. It would be advantageous for clinicians to know the predilection of resorption in varying canine positions. The aim of the study is to evaluate and correlate the severity of root resorption of adjacent teeth and the level of difficulty of impacted canine classified by KPG Index and sector analysis using cone-beam computed tomography. Material and Methods: The study sample comprised 48 impacted canines in orthodontic patients (17 boys and 27 girls) with a mean age of 24 years. The cone-beam computed tomographs were evaluated using Dolphin Imaging Software and primarily, three observations were recorded: KPG index, sector analysis, and root resorption. The samples were equally divided into four groups based on the KPG index, with 12 samples in each group. Additionally, the inclination of the canine in the frontal plane and their degree of vertical eruption were recorded. Results: The number and severity of resorptive lesions increased as the KPG score escalated. The mean KPG score was 13.67 ± 6.336 and the average KPG index was 2.5 ± 1.13. A statistically significant positive correlation was found between all the parameters and root resorption. Conclusion: A positive correlation exists between the level of difficulty of canine impaction and root resorption of the adjacent teeth. A KPG score higher than 13.67 can be a resourceful predictor of resorptive lesions. An increased tendency of root resorption of the neighboring teeth was observed when angle α and angle β exceed 43.71° and 43.26°, respectively.

Effects of green tea extract epigallocatechin-3-gallate (EGCG) on orthodontic tooth movement and root resorption in rats

ObjectiveTo study the effect of EGCG on tooth movement and root resorption during orthodontic treatment in rats. MethodsA total of thirty six male Wistar rats were randomly and equally divided into three groups: control, 50 mg/kg EGCG, and 100 mg/kg EGCG. During the experiment, the subjects were submitted to an orthodontic tooth movement (OTM) model, rats in the experimental groups were given the corresponding dose of EGCG, while rats in the control group were administrated with an equal volume of normal saline solution by gavage. After 14 days of OTM, the rats were sacrificed by transcardial perfusion. Micro-CT of rat maxillaes was taken to analyze OTM distance and root resorption. The maxillary samples were prepared as histological sections for H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemical (IHC) staining to be observed and analyzed. ResultsThe OTM distance and root resorption of rats in the dosed group decreased, and the number of TRAP positive cells in their periodontium decreased significantly. The expression level of RANKL was decreased in the EGCG group compared to the control group, while that of OPG, OCN and Runx2 was increased. Effects were more pronounced in 100 mg/kg group than in 50 mg/kg group. ConclusionEGCG reduces OTM and orthodontic induced root resorption (OIRR) in rats, and is able to attenuate osteoclastogenesis on the pressure side and promote osteogenesis on the tension side.

Comparative Evaluation of Effect of Micro-Osteoperforation and Mechanical Vibration on Rate of Orthodontic Tooth Movement in Young Adults With Bimaxillary Protrusion.

To evaluate and compare the rate of orthodontic tooth movement and root resorption by micro-osteoperforation (MOP) and mechanical vibration in young adults with bimaxillary protrusion. Twenty patients having class I bimaxillary protrusion who required all first premolar extraction were allocated into two groups MOP (Group A) and mechanical vibration (Group B), with a 1:1 allocation ratio. After leveling alignment MOP was performed on either side of the arch, and vibration was applied on the contralateral side 20 mins per day. Canines were retracted with nickel-titanium coil springs, and Alginate impressions were taken every four weeks till 4 months. The mean rate of retraction of canines of Group A was more than Group B. There was a statistically significant difference between Group A and Group B. (p=0.0120) Conclusion: The mean rate of retraction of canines treated by MOP was 1.15 mm per 4 weeks, and by mechanical vibration, 0.8mm per 4 weeks.

Apical Root Resorption of Endodontically Treated Teeth after Orthodontic Treatment: A Split-mouth Study

The influence of pulp status on orthodontically induced root resorption has attracted attention. The purpose of this study was to compare orthodontically induced root resorption in endodontically treated teeth and their contralateral vital teeth in a split-mouth design. The sample included 173 patients who had at least one endodontically treated tooth, and their vital contralateral teeth served as the control group before the completion of orthodontic treatment. Apical root resorption measurements were performed by the comparison of digital panoramic X-ray images obtained at the beginning and at the end of the orthodontic treatment. Kruskal-Wallis, Mann-Whitney U, and Wilcoxon tests were used for statistical analysis. There was no statistically significant difference in apical root resorption between the endodontically treated teeth and the contralateral teeth (p>0.05). Sex and tooth type had no effect on apical root resorption both in the endodontically treated teeth and the contralateral vital teeth (p>0.05). Orthodontic treatment with extraction caused more apical root resorption in the vital teeth than in the endodontically treated teeth (p<0.05). The quality of the endodontic treatment had no significant influence on apical root resorption (p>0.05). Endodontic treatment does not produce greater apical root resorption compared with the vital teeth.

The Impact of Orthodontic Forces on the Occurrence of Iatrogenic Tooth Root Resorption

Modern orthodontic treatment ensures optimal functional and aesthetic results but does not exclude the risk of various complications, including external or internal resorption of tooth roots. One of the causes of root resorption is the impact of orthodontic forces during treatment with both non-removable (brace-system) and removable (aligners) appliances. The aforementioned complication of the treatment of occlusal anomalies, which occurs with a fairly high frequency, potentially threatens not only the patient's health but also complicates the treatment outcomes and worsens the prognosis. The lack of pathognomonic symptoms of root resorption increases the difficulty of an accurate diagnosis. According to the literature, the issue of root resorption is very relevant in clinical dentistry but has not been studied in detail, and contradictory opinions can be found.

Strontium Ranelate Inhibits Osteoclastogenesis through NF-κB-Pathway-Dependent Autophagy.

Strontium ranelate (SR) is a pharmaceutical agent used for the prevention and treatment of osteoporosis and fragility fracture. However, little attention has been paid to the effect of SR on alveolar bone remodeling during orthodontic tooth movement and its underlying mechanism. Here, we investigated the influence of SR on orthodontic tooth movement and tooth resorption in Sprague-Dawley rats and the relationship between the nuclear factor-kappa B (NF-κB) pathway, autophagy, and osteoclastogenesis after the administration of SR in vitro and in vivo. In this study, it was found that SR reduced the expression of autophagy-related proteins at the pressure side of the first molars during orthodontic tooth movement. Similarly, the expression of these autophagy-related proteins and the size and number of autophagosomes were downregulated by SR in vitro. The results also showed that SR reduced the number of osteoclasts and suppressed orthodontic tooth movement and root resorption in rats, which could be partially restored using rapamycin, an autophagy inducer. Autophagy was attenuated after pre-osteoclasts were treated with Bay 11-7082, an NF-κB pathway inhibitor, while SR reduced the expression of the proteins central to the NF-κB pathway. Collectively, this study revealed that SR might suppress osteoclastogenesis through NF-κB-pathway-dependent autophagy, resulting in the inhibition of orthodontic tooth movement and root resorption in rats, which might offer a new insight into the treatment of malocclusion and bone metabolic diseases.

ResMIBCU-Net: an encoder-decoder network with residual blocks, modified inverted residual block, and bi-directional ConvLSTM for impacted tooth segmentation in panoramic X-ray images.

Impacted tooth is a common problem that can occur at any age, causing tooth decay, root resorption, and pain in the later stages. In recent years, major advances have been made in medical imaging segmentation using deep convolutional neural network-based networks. In this study, we report on the development of an artificial intelligence system for the automatic identification of impacted tooth from panoramic dental X-ray images. Among existing networks, in medical imaging segmentation, U-Net architectures are widely implemented. In this article, for dental X-ray image segmentation, blocks and convolutional block structures using inverted residual blocks are upgraded by taking advantage of U-Net's network capacity-intensive connections. At the same time, we propose a method for jumping connections in which bi-directional convolution long short-term memory is used instead of a simple connection. Assessment of the proposed artificial intelligence model performance was evaluated with accuracy, F1-score, intersection over union, and recall. In the proposed method, experimental results are obtained with 99.82% accuracy, 91.59% F1-score, 84.48% intersection over union, and 90.71% recall. Our findings show that our artificial intelligence system could help with future diagnostic support in clinical practice.

Apoptosis in physiological root resorption of primary teeth.

During physiological root resorption of deciduous teeth, apoptotic cell death triggered by physiological processes might play a role in physiological root resorption in addition to collagen destruction. Little information has been obtained about the sequence of events and the mechanism responsible for the physiological death of pulp tissue cells. This study evaluated apoptotic cell death in the pulp tissue of deciduous teeth that showed various levels of physiological root resorption. The role of apoptosis in pulp tissue elimination during the physiological resorption of deciduous teeth was also examined. For orthodontic reasons, 12 healthy permanent teeth and the pulp of 34 healthy deciduous teeth showing signs of early and advanced root resorption were extracted. To detect apoptotic cells in the pulp tissue, the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) method and transmission electron microscopy (TEM) were used. The apoptotic index (AI) values of the study groups were determined using the TUNEL method. This technique required calculating the Apoptag positive(+) fibroblast cell ratio in accordance with the total number of cells. No statistically significant differences were found for the AI values of each study group (p>0.05). Apoptosis was detected in the vascular endothelial cells, the mononuclear inflammatory cells, and the odontoblasts of the connective pulp tissue. In the pulp tissue, evaluated using TEM, various pulp cells were observed at distinct stages of apoptosis. The similarity between the AI values for both study groups suggested that in early and advanced stages of resorption, apoptosis may contribute to the regulation of the pulp cell population in a way that does not relate to the physiological process of deciduous teeth root resorption.

An Assessment and Comparison of Root Resorption With Two Different Corticotomy Techniques During Anterior Teeth Retraction: A Split Mouth Prospective Clinical Study.

Aim The aim of this prospective split-mouth clinical study is to determine the apical root resorption of anterior teeth in patients with two different methods of corticotomy namely indentation and vertical corticotomy techniques. Methods Sixteen adult patients with bimaxillary protrusion requiring the need for extraction of the first premolars were included in the study. For each patient, the particular method of corticotomy technique was assigned randomly along with the side and the procedure was performed following which retraction forces were applied with the help of a closed coil Niti spring. The root resorption was recorded during the stage of space closure for which two cone beam computed tomography (CBCT) were taken, one before the retraction forces could be applied (T1) and one CBCT (T2) after the space closure had occurred. The apical root resorption was assessed and analyzed both linearly and volumetrically using the CBCT taken at T1 and T2. Results There was root resorption present after the space closure was complete in both the maxillary and mandibular arches. The amount of root resorption that occurred in the indentation technique was slightly lesser as compared to the vertical corticotomy technique. Conclusion Indentation corticotomy cuts for accelerated tooth movement are considered to be a much safer, more effective method, lesser invasive to the surrounding tissues, technique sensitive, and goodregional acceleratory phenomenon (RAP), with a Rapid healing process compared to the vertical corticotomy technique.

Inactivation of Sufu in cementoblasts accelerates external tooth root resorption.

Cementum has been empirically regarded as an antiresorptive barrier against tooth roots. However, little is known about the factors of homeostasis and resistant mechanisms of tooth roots against resorption. Here, we investigated cementum factors and their interaction against resorption using transgenic mice exhibiting external cervical root resorption (ECRR). Ectopically thickened cervical cementum caused by functional inactivation of ectonucleotide pyrophosphotase/phosphodiesterase 1 (Enpp1) was susceptible to ECRR with aging. In addition, the inactivation of the suppressor of fused (Sufu), a Hedgehog signaling inhibitor, in cementoblasts led to ECRR. Interestingly, concurrent inactivation of Sufu and Enpp1 in cementoblasts remarkably exacerbated ECRR with higher Rankl expression. Cellular and molecular analyses using cementoblasts and bone marrow-derived macrophages indicated that Dickkopf-related protein 1 (Dkk1) induced by the inactivation of Sufu in cementoblasts has roles in the acceleration of ECRR triggered by Enpp1 inactivation. Using compound mutant mice for concurrent Wntless and Enpp1 inactivation, this synergistic cooperation of Dkk1 and Npp1 for resorption found in double mutant Sufu and Enpp1 mice was confirmed by the reproduction of amplified ECRR. On the basis of these findings, we conclude that proper Npp1 function and sustained Wnt activity in the cervical cementum are essential for the homeostasis of tooth roots against resorption in a physiological state.