Rooting Of Cuttings Research Articles

Penggunaan Bagian Stek Umbi Tanaman Gembili (Dioscorea esculenta) dan Model Lanjaran Terhadap Produksinya

Lesser yam (Dioscorea esculenta) is a root crop which grows and spreads in various parts of Indonesia, from Papua to Sumatra. Plant support technique using stakes is a way to support physical plant growth to control shape, size, and direction of plants. This study aimed to obtain the tuber parts as propagules of lesser yam plant (Dioscorea esculenta) and the of stakes as plant support on lesser yam yield. The research experiment was carried out using a randomized block design (RBD) which consisted of 2 treatment factors; the first factor being the plant support techniques consisted of no support, single support, and pyramidal support model, and the second factor tuber parts as propagules consisted of root, middle, and tip root cuttings. The results showed that the interaction between the tuber cuttings and the plant support model was not significant or there was no synergistic in supporting the growth and yield in the form tubers, whereas the effect of tuber cutting treatments was significant on number of tubers (12 planted tubers) and highly significant on tuber weight (1864.9 g), tuber diameter (5.84 cm) and tuber length (6.11 cm). The effect of plant support models was not significant on number and weight of tubers, however was significant on the tuber diameter (5,54cm), and highly significant on the tuber length (6.05 cm).

ROOTING OF JUVENILE CUTTINGS OF Pinus patula Schiede ex Schltdl. et Cham. HEDGES

Pinus patula Schiede ex Schltdl. et Cham. is endemic to Mexico and is used in both reforestation programs and in the timber industry due to its fast growth, wood quality, and easy handling. With the advance in genetic breeding programs, there is a need to massively propagate new plants from cuttings. One of the main challenges for this purpose is to know the effect of the age of the mother plant (hedge) on the rooting of cuttings. The younger the plant, the greater the rooting is expected to be. Three ontological ages of P. patula mother plants were compared (7, 10, and 13 months). The experimental design was in complete random blocks, with four replications and 25 cuttings per experimental unit. The response variables were evaluated 20 weeks after the experiment was established. No significant differences were observed in the rooting of the three ages evaluated (67.3 to 81 %), nor in the quality of the root (number and length of primary roots, percentage of plants with secondary roots). The results point out an advantage for the propagation of Pinus cuttings since the mother plants at those ages are capable of generating juvenile cuttings that are adequate to obtain percentages of rooting of over 75 %.

Quercus virginianaMill. Root Regrowth Following Linear Trenching

AbstractBackgroundAs long-lived organisms, urban trees often encounter development and redevelopment activities during their lifespans. These activities can damage tree roots, often through methods like root severing during trenching or excavation.MethodsIn 2017, we simulated trenching damage on matureQuercus virginianaMill. trees at 3 different distances from the base (3, 6, or 12 times the stem diameter). After 5 years, we revisited these trees to assess root regrowth based on the cut root’s cross-sectional area (CSA) and distance from the base.ResultsWe observed regrowth in all but 38 (6.7%) of the 557 cut roots revisited. The lack of regrowth in some roots was not associated with our original treatments, the CSA of the roots at the time of trenching, or distance between the cut root end and the trunk (minimumP-value = 0.841). On average, the observed CSA of the regrowth was 22.2% of the original root’s CSA. Only our initial trenching treatments (P-value = 0.024) and the distance between the trunk and the cut root end (P-value = 0.002) significantly predicted the level of regrowth observed 5 years after pruning.ConclusionsIn summary, our findings indicate that root systems require many years to recover from trenching damage. Increasing the distance between trenching activities and trees may have a minor effect on root regrowth.

Effects of Photoperiod Treatments on Stock Plants and Cutting Rooting of Three Cultivars of Ornamental Perennials

Many species of herbaceous perennials now have numerous cultivars, with growth habits and flower colors unique to each cultivar. Vegetative propagation is required so that resulting plants are genetically identical to the parent plant. Although many cultivars are selected for precocious and vigorous flowering, it is often difficult to collect adequate vegetative cuttings from such cultivars for commercial production because juvenile (vegetative) growth is preferred for high-quality cuttings. Cuttings that are reproductive (with flower buds or flowers) can have reduced or delayed rooting and increased occurrences of fungal pathogens (especially Botrytis species), resulting in lack of crop uniformity. We sought to answer the question, can growing stock plants of herbaceous perennials under defined photoperiods extend the length of the vegetative period and enhance the rooting of cuttings harvested from these stock plants? In this study, stock plants of ‘P009S’ twinspur (Diascia integerrima), ‘Furman's Red’ sage (Salvia greggii), and ‘Wild Thing’ sage (Salvia greggii) were grown under ambient, 12-hour light, 10-hour light, and 8-hour light to determine if a particular photoperiod could be used to suppress reproductive growth by promoting vegetative growth, thereby enhancing cutting rooting success. Effects of photoperiod treatments varied among the plant cultivars studied. Plants grown under 8-hour photoperiod had longer duration of vegetative growth, smaller growth rates, and lower dry weights when compared with plants grown under 12-hour or 10-hour photoperiod. Plants grown under 12-hour photoperiod had shorter duration of vegetative growth, larger growth rates, and higher dry weights when compared with plants grown under 10-hour and 8-hour photoperiods. The probability of rooting of cuttings harvested from stock plants of ‘P009S’ twinspur, ‘Furman’s Red’ sage, and ‘Wild Thing’ sage grown under 12-hour and 10-hour photoperiods was greater when compared with cuttings harvested from stock plants grown under 8 h photoperiod.

Growth regulators on shooting and adventitious rooting of Mimosa caesalpiniifolia adult stem cuttings

Mimosa caesalpiniifolia, crucial for fencing and firewood in Brazil's Caatinga dry forest, presents a promising avenue for commercial propagation. The challenges faced in seed propagation, such as the scarcity of inputs and the difficulties in overcoming dormancy, highlight the advantages of vegetative methods. These methods emerge as a viable alternative, enabling uniform and large-scale production of clonal plantlets. This technique ensures consistent plant quality, which is crucial given the wide variety of uses for this species. Additionally, the use of plant growth regulators is critical to enhance the success of vegetative propagation, promoting the formation of robust roots and overall development of plants from cuttings of adult trees. Recognizing the significance of creating clonal forests of M. caesalpiniifolia and the necessity for understanding methods to expedite this endeavor, this study was designed to assess the shooting and adventitious rooting of stem cuttings sourced from adult M. caesalpiniifolia trees, examining the effects of auxinic agents. M. caesalpiniifolia cuttings were harvested through vegetative rescue from six randomly selected adult trees, treated with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) at concentrations of 0, 1,000, 2,000, and 4000 mg L−1, using a completely randomized design in a 2 × 4 factorial arrangement. The evaluations included assessing root presence, number of shoots, vigor of clonal plantlets and plant survival under greenhouse conditions, shade house conditions, and full sun exposure. The study did not reveal any significant interaction between growth regulators and their concentrations under these conditions. IBA at intermediate concentrations enhanced root growth, while cuttings without growth regulators exhibited superior aerial development. A decrease in survival percentages with increasing concentrations of regulators was observed, while cuttings without regulators demonstrated better morphological development. The study suggests for large-scale nurseries, natural rooting and shoot formation in M. caesalpiniifolia cuttings may suffice for successful propagation without IBA or IAA.

Comparison of the efficiency of synthetic auxins and biostimulants and two types of substrate in rooting of shoot cuttings in ‘Pi-ku 1’ rootstock

The low-cost propagation of semi-dwarf cherry rootstock is an essential issue in the production of maiden trees of this species. Among the promising rootstocks is ‘Pi-ku 1’, obtained in Germany. However, the possibility of its propagation using shoot cuttings has not been investigated. It was the purpose of this study. Two ways of cuttings treatment before placing them in the substrate were assessed. One used two preparations in powder (Rhizopon AA and Ukorzeniacz AB), and the second used two alcoholic auxin solutions (IAA and IBA). Instead of synthetic auxins, foliar spraying with two biostimulants was performed (Goteo and Bispeed). All the applied treatments increased the rooting percentage of ‘Pi-ku 1’ rootstock cuttings compared to the control from 5% for Rhizopon AA to 18.2% for auxin IBA. The exception was the lack of a positive effect of the Bispeed biostimulant (less than 5.2%). The use of synthetic auxins increased the number of utting roots more than three times (IBA) or almost twice (Ukorzeniacz AB) and their length more than twofold (IAA, Rhizopon AA) in proportion to the control. The effect of synthetic auxin treatments on the remaining growth parameters under study was also positive. Foliar treatment of cuttings with biostimulants did not change their growth. Only in one of the two years of the study did the fresh mass of cuttings improve after using Goteo biostimulant (7.5%). As part of the experiment, the effect of two substrate types – peat mixed with perlite and peat with sand – was also tested. During the two years of research, the cuttings were rooted several percent better in peat and sand (2.7% – 2018 and 4.4% – 2019). Using peat with sand as a rooting substrate significantly improved the number and length of roots (11.90 and 125.10) of Pi-ku 1 rootstock cuttings compared to the second one (9.23 and 109.08, respectively). All treatments applied to cuttings, except two biostimulants, increased the amount of chlorophyll in the leaves.

Investigation of the Effect of Indolyl Acetic Acid and Naphthenic Acid on the Root Development of Olive Cuttings

The influence of growth factors on the development of callus and roots in the biennial cuttings of the olive plants were investigated in a comparative manner. The experiments were conducted in the greenhouse of the Absheron Experimental Station. One-year and two-year cuttings of the olive plant were taken as the object of the study. Growth agents (indolyl acetic acid and Na+-salt of naphthenic acid) were used to influence the rapid rooting of shoots. During the conducted experiments, different results were obtained in different options. In comparison with the control variant, the positive effect of indolyl acetic acid and Na+-salt of naphthenic acid on olive cuttings were observed. According to the conducted studies, it was found that the rooting of the cuttings was accelerated as a result of the effect of growth substances. The number of rooting and callus was higher in two-year cuttings than in one-year cuttings. Thus, 49 out of 60 (81%) two-year cuttings were rooted by using of indolyl acetic acid, and 50 were rooted (83%) when callus was exposed to the Na+-salt of naphthenic acid, in 9 were observed having callus, only in 1 wasn’t observed development. The Na+-salt of Naphthenic acid had a better effect on rooting and the number of calluses among growth substances. As a result, it was determined that for the rapid development of the olive plant, cuttings should be taken from healthy and well-nourished trees and Naphthenic acid Na+-salt should be kept for a specified time (12 hours) and concentration (0.005%)

VEGETATIVE PROPAGATION FROM SHOOT CUTTINGS OF MATURE ROSEWOOD TREES (ANIBA ROSAEODORA DUCKE)

The production of seedlings by cuttings is an alternative to reconcile exploration and conservation of Rosewood (Aniba rosaeodora Ducke). In this study, our objective was to evaluate the rooting of cuttings from epicormic shoots of adult rosewood strains, through substrate tests, auxin concentrations, and cutting types, as well as to describe the anatomical process of the formation of adventitious roots. We carried out two experiments: E1 - substrate test (sand [A], vermiculite [V], and A+V) and concentrations of indole butyric acid (IBA) via talc (0.0; 1.5; 3.0; 6 .0 g kg-1); E2 – evaluation of the type of cutting: apical, intermediate, basal and regrowth; in addition to anatomical analyzes on samples of rooted cuttings. In the experiments, the variables survival (%), rooting (%), number of roots, and length of the largest root (cm) were evaluated. In E2, shoots (%), number of shoots, length of the largest shoot (cm), and callosity (%) were added. The rooting at 3.0 g kg-1 IBA was better than the control and vermiculite as a substrate was superior to sand. Even without interaction, auxin showed a tendency for better rooting when used with one of the vermiculite substrates. Among the types of cuttings, the regrowth was superior for most variables, differing from the others in terms of sprouting and callosity, while the basal was inferior in the concentration of total carbohydrates and starch.

Morphological, cytological, physiological, and molecular evidence to explain how underground fleshy roots expand in herbaceous peony (Paeonia lactiflora Pall.)

The fleshy roots of herbaceous peonies serve as vital storage organs and are the primary source material for Chinese herbal medicine. Moreover, in certain cultivars, these roots can be propagated by root cuttings. However, the specific mechanisms governing the expansion and development of fleshy roots in herbaceous peonies currently remain unclear. To elucidate patterns of changes during the enlargement and development of herbaceous peony fleshy roots, this study assessed fleshy roots in six growth and development periods (P1–P6). By integrating environmental monitoring data, morphological, cytological, physiological and biochemical, as well as molecular characteristics across these developmental periods were compared. Both precipitation and temperature influenced the development of fleshy roots. The fastest enlargement of fleshy roots occurred under the following growth conditions: daily average precipitation = 5.6 mm; monthly average air temperature = 25.6°C; monthly average soil temperature = 26.3°C. Phloem and xylem, produced by the activity of vascular cambium, are important components of fleshy roots. Secondary xylem thickness consistently increased across the six developmental periods, resulting in the radial expansion of fleshy roots. When average monthly air and soil temperatures were about 26°C, starch accumulated gradually in fleshy roots, and carbohydrates were mostly starch-based. However, when the average monthly air and soil temperatures decreased to about 11.0°C, soluble sugar content increased. Endogenous hormones such as IAA, ZR, GA3, MeJA, and ABA played regulatory roles throughout the development of fleshy roots, and their levels and dynamic equilibrium were correlated with expansion rate. Elevated IAA and ZR content, and stabilized IAA/ABA and MeJA/ABA ratios, favored fleshy root expansion. Trends in the expression of three genes that play key roles in the expansion of fleshy roots (PlEXLB3, PlAUX1 and PlWRKY13) were consistent with morphological and physiological data, but PlHXK1 and PlIPT3 also play important roles. These findings lay a foundation for studying, in greater depth, the regulatory mechanisms governing the enlargement of herbaceous peony fleshy roots, and offer practical advice pertaining to field cultivation and management practices of this ornamental plant.

Effect of two different base cuts and indole-3-butyric acid (IBA) doses on rooting of stem cuttings of wild Silene compacta Fischer

Silene compacta Fisch. ex Hornem., which grows naturally, is a plant species with high ornamental plant potential in the Silene L. genus of the Caryophyllaceae family. Vegetative propagation is one of the important ways to preserve naturally growing and cultivated plant species. In this study, the effect of two different cutting areas of the cutting base (above the node-below the node) and five different indole-3-butyric acid (IBA) doses (control, 500, 1000, 2000 and 4000 ppm) on the rooting of stem cuttings taken from individuals of the natural S. compacta species were investigated. IBA was applied to the cuttings’ bases in a commercial rooting powder. Perlite+peat mixture at a ratio of 1:1 (v/v) was used in the rooting medium. Survival rate, rooting percentage, blooming rate, root number, root length, seedling height, seedling stem diameter, side shoot number, shoot length and shoot stem diameter were evaluated. The evaluation was made one month after the start of the experiment. As a result, the highest survival rate in cuttings was obtained from above-node control group (100%) and under-node 2000 ppm IBA (95%) application, and the highest rooting percentage was obtained from under-node 2000 ppm IBA (68%) and above-node control group (65%), respectively. Based on the results, it can be concluded that different cuttings of the cutting base and IBA concentrations have different effects on the rooting success of the S. compacta species with stem cuttings.

Growth, vegetative rescue and vegetative propagation in Eucalyptus species and hybrids

The objective of the study was the evaluation of survival and productivity indicators in different species/hybrids of Eucalyptus in the adult stage, as well as the vegetative rescue of adult trees and cuttings, testing for species/hybrids, concentration of growth regulator and substrates in rooting. In the evaluation of species/hybrids, height (m), diameter at breast height (DBH, cm) and individual volume (m3 per individual) were measured, and survival was evaluated. In the vegetative rescue treatment, the number of shoots produced at 90, 120 and 210 days was evaluated. In the cutting experiments, the species that produced rescue shoots were tested, in addition to indole-3-butyric acid (IBA) doses (0, 1 500, 3 000 and 4 500 mg L−1) and four substrates for rooting cuttings. The experiments of IBA doses and substrates were carried out using Eucalyptus saligna plants. In the cutting experiments, after 120 days of cultivation, the following variables were evaluated: callus formation (%), survival (%), rooting (%), number and length of roots. The best results regarding survival and quantitative variables of the species/hybrids were obtained for E. dunnii, E. dunnii × E. grandis and E. pellita. In the vegetative rescue, E. dunnii × E. grandis and E. pellita had the highest formation of shoots and the girdling vegetative rescue method had the best results in terms of generated shoots. E. saligna had better results regarding the variables related to the rooting of cuttings. The substrate S3 and the use of 1 500 mg L−1 of IBA provided the highest survival rate and better variables related to the rooting of the cuttings.

Treatments of Coleus forskholii that increase rooting to get larger crude forskolin extract

The aim is study of effect of some phytohormones and microelements in a tissue culture system to extract forskolin by different solvents from hairy roots in Plectranthus barbatus Andr. (Syn.Coleus forskohlii Briq.). Our results indicated that, the best parameters for increasing the number and length of roots were by indole butyric acid. Low concentrations of boron and silicon also promote rooting of Coleus cuttings. Methanol is a more efficient solvent than ethanol and ethyl acetate in forskolin extraction. Root hairs of the terminal cuttings of Coleus forskohlii appear after treatment with bacterial inoculum Agrobacterium rhizogenes. Forskolin levels increased to 13 and 5.3 mg/L in hair roots after 4 weeks of incubation. Forskolin extraction from tissue culture media is more efficient (40 mg/L per g wet weight) compared to extraction from root cuttings (13 mg/10 g wet weight).

Evaluation of the evolutionary process within Populus caspica species from Hyrcanian forests by karyotype analysis

Caspian poplar (Populus caspica Bornm.) is distributed exclusively in the Hyrcanian forests. Hyrcanian forests are the final remnants of Tertiary temperate deciduous forests in Western Eurasia and worldwide. This species plays a significant ecological role in the protection of the natural environment in Hyrcanian forests. In this research, chromosome number and karyotype details of 11 populations of the species were investigated for the first time, using fresh root cuttings collected from mature trees in different parts of the forest, located in the northern parts of Iran. Pre-treatment, fixation, hydrolyzing, and staining were conducted by α-bromonaphthalene, carnoy’s solution, 1N HCl, and hematoxylin agent, respectively. Chromosomal data were analyzed according to a nested model based on a completely randomized design. Chromosome numbers of all of the populations were the same as 2n = 38, which mostly were medium region and sub-metacentric types. Significant differences were observed between the provinces and populations, based on chromosome length grand means, arm ratios and centromere indices. The results demonstrated that structural rearrangement has occurred within the studied populations and indicated an active evolutionary process within and between populations of the species due to natural hybridization. Also, these results showed that artificial inter-specific hybridization between the P. caspica and its relative species can be employed to broaden the ecological zone of the species.

Incision in the base and indolebutyric acid to promote the rooting of 'Bengal' litchi cuttings

A alternative to producing litchi (Litchi sinensis Sonn.) seedlings is by cuttings. However, the main drawback of using this method is related to the difficulty of rooting presented by this species. Thus, the objective of this study was to evaluate the rooting of semihardwood cuttings of litchi ‘Bengal’ at different concentrations of indolebutyric acid, with or without incision in the base. The experimental design was completely randomized with five replicates of 10 cuttings in a factorial 3X2 (three IBA concentrations: 0, 1.000 and 2.000 mg L-1; with or without incision in the base). After 126 days, the variables evaluated were: cutting survival, leaf retention, cutting with callus not rooted, rooted cutting, number of roots per cutting, length of major roots per cutting and dry weight of roots per cutting. For rooting, the IBA application was efficient only when the cuttings were not injured. In addition, there was no influence of the types of cuttings in the IBA concentrations. However, in those with incision at the base, the means were higher without the application of the plant growth regulator. Thus, the 'Bengal' litchi can be multiplied by semihardwood cuttings with incision at the base of the cuttings, without the use of IBA.

Effect of different soil media on rooting of dragon fruit cuttings

The present study was carried out at the Fruit Nursery, Department of Fruit Science, Dr Panjabrao Deshmukh Krishi Vidypeeth, Akola.With the aim to investigate the effect of various compositions of soil media and to find the proper composition for better rooting of dragon fruit cuttings. The design of experiment was CRD with five replications consisting of seven treatment combinations viz., T1-Soil:Sand:FYM, T2-Soil:Red Soil:FYM, T3-Soil:Silt:FYM, T4-Soil:Sand:Vermicompost, T5-Soil:Red Soil:Vermicompost, T6-Soil:Silt:Vermicompost with ratio of 2:1:1 and T7 was control (100% Soil). All the data were recorded on different growth parameters of selected representative cuttings which were selected at random from each replication. Treatment T5 i.e. Soil:Red Soil:Vermicompost in ratio 2:1:1 displayed superior performance articulated in terms of root growth parameters viz., number of root per cutting (58.2), root length (21.54 cm), root diameter (2.36 mm), length of longest root (23.66 cm), average number of roots (53.62), average length of root (20.96 cm), root volume (1.82 cc), fresh weight of root (5.18 g), dry weight of root (0.86 g) and survival percentage (97.59%) among different treatments for successful propagation of dragon fruit cuttings.

Effect of brassinosteroids on rooting of the ornamental deciduous shrubs

Brassinosteroids are a developing group of growth regulators. They are a group of steroid hormones involved in plants’ physiological and developmental processes. Among other things, they are responsible for cell wall regeneration or cell elongation. This experiment aimed to examine the effect of rooting stimulants on rhizogenesis in cuttings of two deciduous shrub species: Philadelphus ’Virginal’ and Hydrangea paniculata ’Limelight’. Aqueous solutions of indole-3-butyric acid (IBA) at 200 mg·L–1, Brassinolide (BL) at 0.05% and 24-epibrassinolide (24epiBL) (0.05%) were used in this study. The results obtained showed that both auxin and both of the brassinosteroids used increased the percentage of rooted cuttings almost twice, the degree of rooted cuttings and root length – for BL + IBA – longer roots than the control by 41% in jasmine and by 59% in hydrangea. The growth regulators applied during the rooting of cuttings also caused changes in the organic compound content of plant tissues and the activity of oxidative stress enzymes. The studies and results suggest that brassinosteroids may soon replace the popular rooting stimulants.

Diversity of phenolic compounds, antioxidant capacity, and mineral element content in four fig cultivars (Ficus carica L.) and their correlation with the rooting of hardwood cuttings

We investigated the phenolic compound profile, antioxidant capacity, rooting of cutting and fruit mineral concentration on 15-year-old trees of four Iranian figs (Ficus carica cvs. “Siyah”, “Kashki”, “Shahanjir” and “Sabz”) planted under rain-fed conditions with different fruit skin color, as well as the correlation between these traits. The range of TSS and antioxidant capacity of fruit (without significant differences) was 75.3–76.7°Brix and 87.9–90.3 DPPHsc %, respectively. “Kashki” had the highest total phenolic (TP) (mg GAE/100 g dw) in both the fruit (1964) and the stem bark (1702) and “Sabz” had the lowest flavonoid content (µg CE mg−1 dw) in the fruit (652.3), leaf (1274.5), and stem bark (1501.9). Although the rooting percentage of cuttings was similar in “Siyah”, “Shahanjir”, and “Sabz”, the lowest rooting percentage (62.7%) and the lowest polyphenol oxidase enzyme (PPO) activity (42.5 U.mL−1) were in “Kashki”. Rooting of the cuttings had a direct correlation with PPO activity and catechin and a negative correlation with the TP, vanillin and trans-ferulic acid concentration in cutting bark. These results showed that the fresh cultivar such as “Shahanjir” with outstanding characteristics, such as high potassium and antioxidant content of fruits and good rooting of the cuttings (97.2%) can be recommended for the development programs of light-colored fresh figs. On the other hand, there were two cultivars, “Siyah” and “Kashki” with dark fruit color; due to their content of TP, total flavonoid, quercetin, trans-ferulic acid and high calcium content in the fruit, these should also be considered.

The Age of Citrus Cultivars Affects Rooting, Growth and Development of Cuttings

The article presents the results of research conducted to study the effect of the age of citrus varieties on the rooting, growth and development of cuttings. The article also provides valuable information on determining the phenophases of rhizogenesis in cuttings of citrus plants of different ages.

Review on A Golden Fiber: Jute and Its by Products

The use of agricultural waste for any type of uses is limited in developing countries. For this study we have selected Jute i.e. Golden Fiber. The traditional uses of this have been sustained in packing, carpet backing, biomass energy after few operations, handmade paper etc. The aims of this study were therefore (i) To summarize the production data in India State wise and year wise. (ii) Special and specific properties of whole jute. (iii) Uses of jute as per its part: Jute sticks, Jute Caddies, Jute Leaves, Jute Root Cuttings in different areas. And after summarize all the uses; it is clear that these parts are not used efficiently. There is some gap between farmers and industrial uses. If these parts can be utilized effectively in the development of value added products, it will certainly strengthen the economy of poor farmers and country also.

Successful Grafting of Two Indonesian Clones of Piper nigrum L. with P. colubrinum Link.: Effects of IBA and NAA on Rooting and Effects of BA on Grafting

<p>This study aimed to investigate the effects of auxin types and their concentrations on rooting of the rootstock cuttings and BA on grafting. First, IBA, NAA or IBA+NAA, each at 0, 1000, 2000, and 3000 ppm were used as treatments to study rooting of <em>P</em><em>iper colubrinum</em>. In the second experiment, BA (0 and 50 ppm) was applied to <em>P. nigrum</em> scion clones of Natar-1 and Petaling-2, before being grafted to the rootstocks. Results showed, that all types of auxins (IBA, NAA or IBA+NAA) induced rooting and shoot growth, and the increase of auxin concentrations led to the increase of rooting. However, their effectiveness was different. NAA and IBA+NAA were superior to IBA, and at 2000 ppm, IBA+NAA resulted in the best plant growth, as indicated by higher values of both rooting and shoot growth parameters. Compared to control, BA treatment on scions resulted in higher grafting success (80% vs. 93%) for Natar-1, and (73% vs.100%) for Petaling-2 clones. BA treatment on scions also induced more calluses in the graft union and better shoot growth. To our knowledge, this is the first report on the use of BA to increase success of grafting between <em>P. nigrum</em> and<em> P. </em><em>colubrinum</em>.</p>