Changing Role Research Articles

Total Factor Productivity Growth in the Indian Food Processing Industry: A Non-parametric Investigation

This study analysed total factor productivity growth (TFPG) in the Indian food processing industry in the organised sector. The results showed that TFPG in this industry resulted from technical progress and that the role of change in technical efficiency was insignificant. Therefore, for sustainable output growth, the application of new technologies must be followed by adaptation. In this context, policymakers should consider sub-sectoral heterogeneity when designing policies for the Indian food processing industry so that the rates of applying technology and its adaptability can be matched for each industry sub-sector. Thus, the study offers a strong note of caution about investments in technological improvements.

Effects of transport densities on the physiological and biochemical characteristics of sturgeon (Acipenser baerii ♀ × A. schrenckii ♂)

Transport procedures usually cause fish stress, especially at high densities, which might cause considerable stress and compromise product quality for adult fish. However, many previous studies of density on sturgeon have hindered understanding of its roles in the physiological change during and after the transportation of sturgeon and no study explored high density as fish-to-water ratios up to 1:1. The objective of this study was to investigate the effects of transport density and duration on antioxidant indices, immune response, and post-transported recovery time of adult sturgeon (758.30 ± 42.8 g, one and a half years old) during long-distance and high-density transportation. Sturgeon were anesthetized with MS-222 under different fish-to-water ratios of 1:1 (D1), 1:2 (D2), 1:3 (D3) and 1:4 (D4), and simulated transport was carried out for 0 h, 1 h, 6 h, 24 h, 48 h and 72 h, and resuscitation was carried out for 6 h, 24 h and 48 h after transport. The relevant measurements were made on the water and serum samples. The findings demonstrated that after 72 h of transportation at various densities, the survival rate was 100%. Total ammonium nitrogen concentrations in the water increased in parallel with the stocking density after 48 h. After 72 h of transport, blood levels of malondialdehyde (MDA), glutathione peroxidase (GSH-PX), catalase (CAT), glutamate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and total protein (TP) were substantially higher in group D1 than in group D4 (P < 0.05), although serum levels of MDA, cortisol (COR), TP, and immunoglobulin M (IGM) in group D2 were not statistically different from those in group D3 (P > 0.05). To cope with the pressure imposed by high-density transport, the antioxidant, metabolic, and immunological indexes of sturgeon in the D1 and D2 were significantly greater than those of sturgeon in D4 group during transport. The D1 group had the most severe damage to antioxidant, metabolic, and immune abilities after resuscitation, and at 48 h after resuscitation, the levels of MDA, AST, and IGM were still significantly higher than those in the control group (P<0.05), whereas the levels of IGM in the D2 group were not significantly different (P > 0.05). Sturgeon under anesthesia should not be transported for longer than 72 h at a time with a fish-to-water ratio of 1:1 because doing so resulted in irreparable harm to all fish indicators. To better comprehend the circumstances necessary for efficient transportation, the study may contribute to evaluate the changes in physiological and biochemical characteristics of sturgeon.

European Summer Wet-Bulb Temperature: Spatiotemporal Variations and Potential Drivers

Abstract Heat stress is projected to intensify with global warming, causing significant socioeconomic impacts and threatening human health. Wet-bulb temperature (WBT), which combines temperature and humidity effects, is a useful indicator for assessing regional and global heat stress variability and trends. However, the variations of European WBT and their underlying mechanisms remain unclear. Using observations and reanalysis datasets, we demonstrate a remarkable warming of summer WBT during the period 1958–2021 over Europe. Specifically, the European summer WBT has increased by over 1.0°C in the past 64 years. We find that the increase in European summer WBT is driven by both near-surface warming temperatures and increasing atmospheric moisture content. We identify four dominant modes of European summer WBT variability and investigate their linkage with the large-scale atmospheric circulation and sea surface temperature anomalies. The first two leading modes of the European WBT variability exhibit prominent interdecadal to long-term variations, mainly driven by a circumglobal wave train and concurrent sea surface temperature variations. The last two leading modes of European WBT variability mainly show interannual variations, indicating a direct and rapid response to large-scale atmospheric dynamics and nearby sea surface temperature variations. Further analysis shows the role of global warming and changes in midlatitude circulations in the variations of summer WBT. Our findings can enhance the understanding of plausible drivers of heat stress in Europe and provide valuable insights for regional decision-makers and climate adaptation planning. Significance Statement Wet-bulb temperature, which takes into account the combined effect of temperature and humidity, is a good indicator for assessing heat stress. In the context of global warming, heat stress is anticipated to escalate, posing significant risks to human health and causing socioeconomic losses. However, variations in wet-bulb temperature and the associated physical mechanisms have received limited attention. This study aims to improve our understanding of the temporal and spatial variations and the potential driving mechanisms of summer wet-bulb temperature across Europe in recent decades. We have observed a noteworthy increase in summer wet-bulb temperature, indicating a regional intensification of heat stress, particularly within the last 10 years. We further investigate the connections between variations in summer wet-bulb temperature, large-scale atmospheric circulation, and sea surface temperature. Additionally, we explore their associations with global warming and changes in midlatitude atmospheric circulation. The outcomes of this study not only contribute to establishing a scientific basis for evaluating heat-related risks in Europe but also facilitate preparedness for future climate adaptation and mitigation at both regional and local scales.

The decline of the 2022 Italian mpox epidemic: Role of behavior changes and control strategies

In 2022, a global outbreak of mpox occurred, predominantly impacting men who have sex with men (MSM). The rapid decline of this epidemic is yet to be fully understood. We investigated the Italian outbreak by means of an individual-based mathematical model calibrated to surveillance data. The model accounts for transmission within the MSM sexual contact network, in recreational and sex clubs attended by MSM, and in households. We indicate a strong spontaneous reduction in sexual transmission (61-87%) in affected MSM communities as the possible driving factor for the rapid decline in cases. The MSM sexual contact network was the main responsible for transmission (about 80%), with clubs and households contributing residually. Contact tracing prevented about half of the potential cases, and a higher success rate in tracing contacts could significantly amplify its effectiveness. Notably, immunizing the 23% of MSM with the highest sexual activity (10 or more partners per year) could completely prevent new mpox resurgences. This research underscores the importance of augmenting contact tracing, targeted immunization campaigns of high-risk groups, and fostering reactive behavioral changes as key strategies to manage and prevent the spread of emerging sexually transmitted pathogens like mpox within the MSM community.

Decarbonizing our environment via the promotion of biomass methanation in developing nations: a waste management tool

Abstract For a long time, fossil fuel has been a part of our everyday lives and has constantly led to the emission of carbon dioxide (CO2) into the environment. The release of methane (CH4) into our surroundings can be caused by the decomposition of organic wastes produced by our daily activities; CH4 produced by human activity is responsible for at least 25 % of global warming. CH4 is a known potent greenhouse gas that can trap about 35 times more heat than CO2. These greenhouse gases play a role in climate change and global warming. It, therefore, becomes important to explore measures for decarbonizing our environment. Biomethane production using our generated waste is a promising decarbonization approach with significant potential for mitigating greenhouse gas emissions. This paper overviews potential biomass methanation feedstocks and investigates several technologies, such as anaerobic digestion, combined pyrolysis and methanation, and combined gasification and methanation. SWOT analysis of waste conversion to biomethane was conducted, and important points related to the scaling-up of biomethane production processes were outlined. Also, insights into prospects for promoting biomass methanation deployment were provided. In conclusion, biomass methanation has great potential for producing sustainable energy. Hence, collaboration between industrialists, researchers, government agencies, and stakeholders including an understanding of the financial investments, return on investments, or potential subsidies and incentives could enhance the practicality of the proposed solution. Research and development should be continuously carried out as they are necessary to scale up and promote the technology. Also, there should be technical training for stakeholders as it is essential for the smooth development of the sector.

Climate change is an important predictor of extinction risk on macroevolutionary timescales.

Anthropogenic climate change is increasing rapidly and already impacting biodiversity. Despite its importance in future projections, understanding of the underlying mechanisms by which climate mediates extinction remains limited. We present an integrated approach examining the role of intrinsic traits versus extrinsic climate change in mediating extinction risk for marine invertebrates over the past 485 million years. We found that a combination of physiological traits and the magnitude of climate change is necessary to explain marine invertebrate extinction patterns. Our results suggest that taxa previously identified as extinction resistant may still succumb to extinction if the magnitude of climate change is great enough.

Alzheimer Hastası Bakım Vereninde Kişilerarası Psikoterapi Yaklaşımı: Olgu Sunumu

Alzheimer's Disease, which is turning into a public health problem in elderly societies, is characterized by significant deterioration in cognitive as well as behavioral processes, and is defined as a neurodegenerative disease, has a challenging process, especially for caregivers. The caregiver, who is seen as the main decision-maker in the disease, may experience problems with both the patient and the environment due to the burden of care, lack of knowledge about the disease, and the weakness of support networks. While the caregiver tries to manage the disease process, they also face loss and mourning. For this reason, especially the difficulty in accepting and adapting to changing roles brings about conflicts in relationships with the patient and others. At this point where support mechanisms gain importance, the Interpersonal Based Psychotherapy (IPT) Approach, which will be put into practice constructively, has a positive impact on caregiver relationships and makes the process bearable by reflecting positively on the prognosis of the disease. IPT is a psychotherapeutic approach used on many interpersonal problems and psychiatric syndromes, and works on the areas of interpersonal conflict, grief and loss, and role reversal. This case study aimed to evaluate the IPT Approach for D., who was caring for his father with Alzheimer's Disease. It has been seen that the approach plays an effective role when the caregiver's relationship with the patient and others is evaluated. Observed in literature studies is that positive interventions for caregivers have positive effects on both the patient and the caregiver. Olgu D., who experienced conflict in her relationships due to the weight of the burden of care and existing life responsibilities, along with the diagnosis of her father's illness, gained accurate information about the disease. At the same time with the therapeutic approach, the insight gained as a result of strengthening the support mechanisms, explaining the situation and the need for help, and the positive structuring of the social support network were achieved. Key Words: Alzheimer's Disease, Interpersonal Psychotherapy, Caregiver

Zonal control on Holocene precipitation in northwestern Madagascar based on a stalagmite from Anjohibe

The Malagasy Summer Monsoon is an important part of the larger Indian Ocean and tropical monsoon region. As the effects of global warming play out, changes to precipitation in Madagascar will have important ramifications for the Malagasy people. To help understand how precipitation responds to climate changes we present a long-term Holocene speleothem record from Anjohibe, part of the Andranoboka cave system in northwestern Madagascar. To date, it is the most complete Holocene record from this region and sheds light on the nature of millennial and centennial precipitation changes in this region. We find that over the Holocene, precipitation in northwestern Madagascar is actually in phase with the Northern Hemisphere Asian monsoon on multi-millennial scales, but that during some shorter centennial-scale events such as the 8.2 ka event, Anjohibe exhibits an antiphase precipitation signal to the Northern Hemisphere. The ultimate driver of precipitation changes across the Holocene does not appear to be the meridional migration of the monsoon. Instead, zonal sea surface temperature gradients in the Indian Ocean seem to play a primary role in precipitation changes in northwestern Madagascar.

Bordering Democracies, Democratising Borders

Over the past 30 years, border scholars have written extensively on what borders are, where they are located, and how they operate, not just to critically understand their changing role, but also to criticise and denounce their violence and discrimination. Yet borders continue to proliferate, in particular as a response to alleged crises affecting Europe. If borders have always constituted markers of social and cultural identity, the more recent process of European re-bordering, I argue, constitutes a challenge for the democratic system as a whole. Implemented by left-wing and right-wing parties alike, this process seems indeed to have been taken away from public discourse and treated as a technical necessity to solve the crises. Far from being neutral or non-political, however, it has disclosed new forms of racial discrimination, political and economic power, and colonial violence. In order to substantiate my argument, I will 1) provide a brief examination of the recent changes in the concept and practice of democracy, as well as their interrelations with the process of European re-bordering, 2) investigate the socio-political and economic conditions under which the current process of European re-bordering has come about, with particular attention to the increasing role of media and political discourses in shaping public opinion, and 3) discuss the repercussions of the process of European re-bordering on the democratic system. The article will conclude by inviting scholars, civil society members, and any interested party to open up a more open and democratic debate around the unequal and discriminatory practices of bordering.

Dealing with fibromyalgia in the family context: a qualitative description study

Headings purpose: Fibromyalgia (FM) is a chronic, nondegenerative disease with important limitations in patients. Its average global prevalence is 1.78%, and women are more affected than men (3:1). Due to the lack of objective diagnostic tools, it is a complex medical condition that is frequently unseen by patients’ relatives and doctors, which might nonetheless have a noticeable impact on the patient’s entourage. Material and Methods: This qualitative descriptive study aimed to elicit family members’ views on how FM affects their lives. It was conducted in two community health centers (one rural and one urban) from the Sagunto Health Department (Valencia Community, Spain). We included seven focus groups with 41 family members. We analyzed the data gathered with an inductive thematic semantic analysis approach using NVivo 12 software. Results: We identified four major themes: (1) fibromyalgia as a nosological entity or an invention that is always burdensome; (2) children and spouses as caregivers (or not); (3) adverse effects of fibromyalgia on the couple’s sexual life; and (4) harmful consequences of FM on the family economy. The findings showed a negative impact of the disease within the family context. Family members face complex and changing roles and difficulties when living with women with fibromyalgia. Conclusions: Relatives’ better understanding of the disease, greater acceptance of new family roles, and improvement of patients’ work conditions are all interventions that may help reduce the negative impact of FM in the family context.

The Influence of Vegetation on Climate Elements in Northwestern China

Vegetation plays a crucial role in maintaining the balance between nature, water and soil resources. However, understanding its impact mechanisms in arid and semi-arid areas remains limited. This study aims to analyze the spatial–temporal characteristics of the vegetation leaf area index (LAI) and climate elements in typical regions of northwest China and the correlations between LAI and climate elements; it also aims to explore the influence of regional vegetation growth on climate change. The results reveal significant correlations between LAI and various climate elements. Specifically, within the same region, surface temperature, precipitation, vegetation transpiration, and total evaporation show positive correlations with the LAI, whereas surface albedo shows a negative correlation. Vegetation may affect climate through both heat and water exchange between the land and atmosphere. Increased vegetation leads to the enhanced absorption of solar radiation by the land surface, elevating surface temperature. Increased levels of vegetation also increase vegetation transpiration and total evaporation, increasing the water vapor content in the atmosphere and thus leading to increased surface precipitation. Therefore, vegetation distribution plays a role in climate change, and ecological restoration projects in the northwest region hold significant potential for addressing ecological challenges in its arid and semi-arid areas.

Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

Vernalization is necessary for winter wheat to flower. However, it is unclear whether vernalization is also required for spring wheat, which is frequently sown in fall, and what molecular mechanisms underlie the vernalization response in wheat varieties. In this study, we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat varieties. For this purpose, we determined how major vernalization genes (VRN1, VRN2, and VRN3) respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene expression. We also identified genes that are differentially regulated in response to vernalization in winter and spring wheat varieties. We found that in winter wheat, but not in spring wheat, VRN1 expression decreases when returned to warm temperature following vernalization. This finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3 (H3K27me3) and tri-methylation of lysine 4 on histone H3 (H3K4me3) at the VRN1 gene. Analysis of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes, including those involved in leucine catabolism, cysteine biosynthesis, and flavonoid biosynthesis. These findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.

Genetic determinants of atopic dermatitis

Atopic dermatitis is a heterogeneous disease, the pathogenesis of which is associated with mutations of genes encoding structural proteins of the epidermis, barrier enzymes and their inhibitors.&#x0D; The article analyzes data from the works of various authors on the study of gene expression in atopic dermatitis, and notes the role of genes regulating innate and adaptive immune responses, as well as environmental factors inducing the disease. Recent studies indicate a key role of epigenetic changes in the development of the disease. Epigenetic modifications are mainly mediated by DNA methylation, histone acetylation and the action of specific non-coding RNAs. It has been documented that the profile of epigenetic changes in patients with atopic dermatitis differs from that observed in healthy individuals.&#x0D; Understanding epigenetic changes is critical for developing personalized treatment strategies.

What can be learned from using participatory landscape scenarios in Rio Doce State Park, Brazil?

ContextThe goal of this work is to describe and explain the conditions that trigger, enable or prevent the implementation of preferred land uses by local communities.ObjectiveThe questions this paper addresses are as follows: Is there a set of preferred scenarios for land use change? What triggers, enables and prevents the implementation of preferred land uses? What did we learn by using participatory landscape scenarios?MethodsIn order to answer the research questions, we synthesize knowledge from a long-term case study in the buffer zone of the Rio Doce State Park in Brazil by (1) Developing participatory landscape scenarios and spatially explicit models for exploring desirable futures and associated land uses, as seen by local communities; (2) Explaining the conditions that trigger, enable or prevent the implementation of preferred landscape scenarios; and (3) Synthesizing knowledge on research and practice for working towards preferred land use changes.ResultsPreferred scenarios for land use change are agroforestry, animal husbandry, tourism and conservation. Although there is biophysical potential and funding available through multiple social and environmental programs, the Rio Doce State Park’s institutional setting is confusing. Although preferred land use scenarios will likely be implemented, participatory landscape approaches require a new, strategic role and leadership by regional institutions. Universities need to bring in social theories and institutional analysis to educate a new generation of landscape stewards.ConclusionWe highlight the role of more responsive social agents and their representative institutions, including the changing role of university-based research and practice for working towards sustainable landscape outcomes.

Influence of Weather and Climate on Multidecadal Trends in Atlantic Hurricane Genesis and Tracks

Abstract This study investigates the relative roles of sea surface temperature–forced climate changes and weather variability in driving the observed eastward shift of Atlantic hurricane tracks over the period from 1970 to 2021. A 10-member initial condition ensemble with a ∼25-km horizontal resolution tropical cyclone permitting atmospheric model (GFDL AM2.5-C360) with identical sea surface temperature and radiative forcing time series was analyzed in conjunction with historical hurricane track observations. While a frequency increase was recovered by all the simulations, the observed multidecadal eastward shift in tracks was not robust across the ensemble members, indicating that it included a substantial contribution from weather-scale variability. A statistical model was developed to simulate expected storm tracks based on genesis location and steering flow, and it was used to conduct experiments testing the roles of changing genesis location and changing steering flow in producing the multidecadal weather-driven shifts in storm tracks. These experiments indicated that shifts in genesis location were a substantially larger driver of these multidecadal track changes than changes in steering flow. The substantial impact of weather on tracks indicates that there may be limited predictability for multidecadal track changes like those observed, although basinwide frequency has greater potential for prediction. Additionally, understanding changes in genesis location appears essential to understanding changes in track location. Significance Statement From the 1970s to the present, there has been an increase in the frequency of North Atlantic hurricanes, but they have also shifted in location to the east, away from land. We explore whether this shift in hurricanes’ locations was caused by climatic factors or randomness to understand if and how these trends will persist. We also consider whether the shift was due to a change in where hurricanes started or how they moved over their lifespan. Analyzing data from observed and simulated hurricanes, we find that the shift was made more likely by climate factors, but ultimately occurred due to random variability in the hurricanes’ starting locations. These results suggest a higher uncertainty in the future location and impact of hurricanes and highlight the importance of studying why hurricanes originate where they do.

Exploring Positive Youth Development Approach in Indian Context

Positive youth development (PYD), which arose as a counterbalance to the treatment models in youth work, stresses the role of family, community and other change agents, such as NGOs and educational institutions, in creating services, opportunities and support systems for young people to excel. The article tries to locate the context of PYD approach. Single case study method is employed to document the historical emergence of the Anubhav Shiksha Kendra (ASK) and its adoption of PYD approach. Though the study cannot be generalised, it provides a framework for future empirical studies on PYD in India.

Blood pressure and intensive treatment up-titration after acute heart failure hospitalization: Insights from the STRONG-HF trial.

A high-intensity care (HIC) strategy with rapid guideline-directed medical therapy (GDMT) up-titration and close follow-up visits improved outcomes, compared to usual care (UC), in patients recently hospitalized for acute heart failure (AHF). Hypotension is a major limitation to GDMT implementation. We aimed to assess the impact of baseline systolic blood pressure (SBP) on the effects of HIC versus UC and the role of early SBP changes in STRONG-HF. A total of 1075 patients hospitalized for AHF with SBP ≥100 mmHg were included in STRONG-HF. For the purpose of this post-hoc analysis, patients were stratified by tertiles of baseline SBP (<118, 118-128, and ≥129 mmHg) and, in the HIC arm, by tertiles of changes in SBP from the values measured before discharge to those measured at 1 week after discharge (≥2 mmHg increase, ≤7 mmHg decrease to <2 mmHg increase, and ≥8 mmHg decrease). The primary endpoint was 180-day heart failure rehospitalization or death. The effect of HIC versus UC on the primary endpoint was independent of baseline SBP evaluated as tertiles (pinteraction = 0.77) or as a continuous variable (pinteraction = 0.91). In the HIC arm, patients with increased, stable and decreased SBP at 1 week reached 83.5%, 76.2% and 75.3% of target doses of GDMT at day 90. The risk of the primary endpoint was not significantly different between patients with different SBP changes at 1 week (adjusted p = 0.46). In STRONG-HF, the benefits of HIC versus UC were independent of baseline SBP. Rapid GDMT up-titration was performed also in patients with an early SBP drop, resulting in similar 180-day outcome as compared to patients with stable or increased SBP.

The Role of Rapid Changes in Weather on Phytoplankton Spring Bloom Dynamics From Mid‐Norway Using Multiple Observational Platforms

AbstractThe spring phytoplankton bloom plays a major role in pelagic ecosystems; however, its dynamics are not well understood due to insufficient, highly resolved observational data. Here we investigate the start, peak, and decline of a 2‐week phytoplankton spring bloom in Frohavet located in a biological hotspot of the coast of mid‐Norway. We used observations from an uncrewed surface vehicle (USV) combined with buoy measurements, satellite images, discrete water sampling and modeling approaches. The spring bloom (March–June 2022) consisted of multiple peaks (up to 5 mg Chla m−3), with a long peak in April dominated by the diatom Skeletonema, coincident with the period when the USV captured the temporal and spatial dynamics of the bloom. Short‐term episode of calm weather in spring, such as clear skies and consistent low wind speed (&lt;7 m s−1) shoaled the mixed layer depth (&lt;15 m) and promoted the rapid development of the spring bloom ‐ from 1 to 5 mg Chla m−3 in 5 days. Likewise, the collapse of the bloom was rather quick, 1–2 days and coincides with low nitrate values and rapid increase in wind speed (&gt;10 m s−1), suggesting strong influence of the environment on phytoplankton dynamics. Understanding the dynamics of the spring bloom is crucial for predicting its impact on marine trophic web and biogeochemical cycles. Integration of distinct observational platforms has the potential to unveil the environmental factors underlying phytoplankton bloom dynamics.

Targeted next-generation sequencing and long-read HiFi sequencing provide novel insights into clinically significant KLF1 variants

BackgroundKrüppel-like factor 1 (KLF1), a crucial erythroid transcription factor, plays a significant role in various erythroid changes and haemolytic diseases. The rare erythrocyte Lutheran inhibitor (In(Lu)) blood group phenotype serves as an effective model for identifying KLF1 hypomorphic and loss-of-function variants. In this study, we aimed to analyse the genetic background of the In(Lu) phenotype in a population-based sample group by high-throughput technologies to find potentially clinically significant KLF1 variants.ResultsWe included 62 samples with In(Lu) phenotype, screened from over 300,000 Chinese blood donors. Among them, 36 samples were sequenced using targeted Next Generation Sequencing (NGS), whereas 19 samples were sequenced using High Fidelity (HiFi) technology. In addition, seven samples were simply sequenced using Sanger sequencing. A total of 29 hypomorphic or loss-of-function variants of KLF1 were identified, 21 of which were newly discovered. All new variants discovered by targeted NGS or HiFi sequencing were validated through Sanger sequencing, and the obtained results were found to be consistent. The KLF1 haplotypes of all new variants were further confirmed using clone sequencing or HiFi sequencing. The lack of functional KLF1 variants detected in the four samples indicates the presence of additional regulatory mechanisms. In addition, some samples exhibited BCAM polymorphisms, which encodes antigens of the Lutheran (LU) blood group system. However, no BCAM mutations which leads to the absence of LU proteins were detected.ConclusionsHigh-throughput sequencing methods, particularly HiFi sequencing, were introduced for the first time into genetic analysis of the In(Lu) phenotype. Targeted NGS and HiFi sequencing demonstrated the accuracy of the results, providing additional advantages such as simultaneous analysis of other blood group genes and clarification of haplotypes. Using the In(Lu) phenotype, a powerful model for identifying hypomorphic or loss-of-function KLF1 variants, numerous novel variants have been detected, which have contributed to the comprehensive understanding of KLF1. These clinically significant KLF1 mutations can serve as a valuable reference for the diagnosis of related blood cell diseases.

Coherent long-term body-size responses across all Northwest Atlantic herring populations to warming and environmental change despite contrasting harvest and ecological factors.

Body size is a key component of individual fitness and an important factor in the structure and functioning of populations and ecosystems. Disentangling the effects of environmental change, harvest and intra- and inter-specific trophic effects on body size remains challenging for populations in the wild. Herring in the Northwest Atlantic provide a strong basis for evaluating hypotheses related to these drivers given that they have experienced significant warming and harvest over the past century, while also having been exposed to a wide range of other selective constraints across their range. Using data on mean length-at-age 4 for the sixteen principal populations over a period of 53 cohorts (1962-2014), we fitted a series of empirical models for temporal and between-population variation in the response to changes in sea surface temperature. We find evidence for a unified cross-population response in the form of a parabolic function according to which populations in naturally warmer environments have responded more negatively to increasing temperature compared with those in colder locations. Temporal variation in residuals from this function was highly coherent among populations, further suggesting a common response to a large-scale environmental driver. The synchrony observed in this study system, despite strong differences in harvest and ecological histories among populations and over time, clearly indicates a dominant role of environmental change on size-at-age in wild populations, in contrast to commonly reported effects of fishing. This finding has important implications for the management of fisheries as it indicates that a key trait associated with population productivity may be under considerably less short-term management control than currently assumed. Our study, overall, illustrates the need for a comparative approach within species for inferences concerning the many possible effects on body size of natural and anthropogenic drivers in the wild.