Research Purpose: This study aims to describe the role of mothers in applied three indicators of clean and healthy living behavior at household order in preschool age children (3-6 years) in the working area of Cakranegara Health Center. Methods: This study is an observational descriptive study that using survey methods. Respondents of this study are some mothers who have preschool age children (3-6 years) in the working area of Cakranegara Health Center which totaling 135 people. Conclution: The results of the study at the level of mother's role which contained 58 (43%) of respondents in the enough category, 48 (36%) of respondents in the less category and 29 (21%) of respondents in the good category. Based on the results of the study, expected to parents, especially mothers to uphold the principle role of teaching, modelling, mentoring, organizing and able to maintain a clean and healthy living behavior in children.