Ring-shaped Pattern Research Articles

Generation of mid-infrared vortex beams by 3-D printed polymer phase plates

Light carrying vortex beam characteristics were demonstrated in the mid-infrared (mid-IR) region using polymer based spiral phase plates (SPP). The SPP was fabricated through three dimensional (3-D) direct laser writing method, which can construct the optical phase according to the mid-IR wavelength of interest. The fabricated SPP consisted of 32 layers and the layer thickness was precisely adjusted between 100 and 600 nm to create 2π helical phase change. The ideal SPP structure and the gradient optical phase diagram were designed and simulated by the finite-difference time-domain (FDTD) method. Characteristic vortex beam profiles with a ring-shaped pattern and a sharp centric dark spot were experimentally illustrated at a wavelength range of λ = 3.1–3.3 µm. The formation of mid-IR OAM through high throughput 3-D writing technology provides critical integrated components to explore quantum photonics and optical communications.

Investigation of the location and secretion features of Candida albicans enolase with monoclonal antibodies

PurposeThe glycolytic enzyme enolase plays important role in the pathogenesis of Candida albicans infection and has been also considered as a promising molecular marker for the diagnosis of invasive candidiasis. This study aimed to investigate the location and secretion features of Candida albicans enolase (CaEno) with a couple of specific monoclonal antibodies (mAbs).MethodsTwo mAbs named 9H8 and 10H8 against CaEno were generated by fusing SP2/0 myeloma cell with the spleen lymphocytes from CaEno immunized mice. The specificity of the mAbs was then validated by Western blot and liquid chromatography-mass spectrometry (LC–MS/MS). A diverse set of experiments were conducted based on the pair of mAbs which involved immunohistochemical staining analysis, whole cell enzyme-linked immunosorbent assay (ELISA), double antibody sandwich ELISA, and confocal microscopy to analyze the possible location and secretion features of CaEno.ResultsCaEno is abundantly expressed in the cytoplasm of C. albicans blastospores and is distributed in a ring-shaped pattern along the cell wall. CaEno appeared in the hyphal C. albicans as just a “mushroom” form. CaEno was found to be weakly expressed on the surface of blastospores but constantly expressed at various stages of growth. CaEno concentrations in C. albicans blastospores culture supernatant are considerably higher than in C. albicans hyphae culture supernatant. The dynamic changes of supernatant CaEno concentration in blastospores and hyphal C. albicans exhibit distinct features, although both appear to be associated with the C. albicans growth state. When cultivated under normal circumstances, however, no apparent CaEno degradation was seen in the cell-free supernatant.ConclusionOur results implied that CaEno was constantly expressed on the cell surface and its secretion features varied according to the growth stage of C. albicans. However, further experimental and theoretical studies are needed in future to identify the specific mechanisms by which this phenomenon can arise.

Quantification of Desiccated Extracellular Vesicles by Quartz Crystal Microbalance.

Extracellular vesicle (EV) quantification is a procedure through which the biomedical potential of EVs can be used and their biological function can be understood. The number of EVs isolated from cell culture media depends on the cell status and is especially important in studies on cell-to-cell signaling, disease modeling, drug development, etc. Currently, the methods that can be used to quantify isolated EVs are sparse, and each have limitations. In this report, we introduce the application of a quartz crystal microbalance (QCM) as a biosensor for quantifying EVs in a small drop of volatile solvent after it evaporates and leaves desiccated EVs on the surface of the quartz crystal. The shifts in the crystal’s resonant frequency were found to obey Sauerbrey’s relation for EV quantities up to 6 × 107, and it was determined that the biosensors could resolve samples that differ by at least 2.7 × 105 EVs. A ring-shaped pattern enriched in EVs after the samples had dried on the quartz crystal is also reported and discussed. QCM technology is highly sensitive and only requires small sample volumes and is significantly less costly compared with the approaches that are currently used for EV quantification.

Study on the risk of soil heavy metal pollution in typical developed cities in eastern China

Enrichment of heavy metals in urban soils has become a major regional environmental risk. At present, research on the soil heavy metals in cities lacks risk spatial correlation analyses between different heavy metals, and there is a relative lack of assessments of the ecological and health risks. We selected Wuxi, a typical developed city of eastern China, collected and tested the contents of heavy metals in the urban soils of Wuxi in May 2020. Combined with Pb isotope analysis, ecological and health risk assessment, we found that the high heavy metal concentrations in Wuxi are mainly located in the central and western regions, and that the changes in spatial fluctuation are relatively small. The Pb isotopes in the urban soils of Wuxi are distributed in areas, such as those are related to coal combustion, automobile exhaust and urban garbage, indicating that the heavy metals in the urban soils of Wuxi are affected by human activities such as coal combustion and automobile exhaust. The average value of the potential ecological risk index of soil heavy metal Cd is 80.3 (the threshold: 40), which represents a high-risk state. Whether adults or children, the risk of soil heavy metals via ingestion is much higher than that through skin exposure. High health risk values are present in the central area of Wuxi and decrease in a ring-shaped pattern, which is similar to the population distribution of Wuxi and greatly increases the potential risk from soil heavy metals, which should be given close attention. We should develop and use clean energy to replace petroleum fossil fuels, especially in densely populated areas. This study provides technical support for the prevention and control of urban heavy metal pollution.

Gold nanoparticle ring arrays from core-satellite nanostructures made to order by hydrogen bond interactions.

Polyethylene glycol-grafted gold nanoparticles are attached to silica nanoparticle cores via hydrogen bonding in a controlled fashion, forming well-defined core–satellite structures in colloidal solution. For separating these complex structures effectively from the parental nanoparticles, a straightforward and easy protocol using glass beads has been developed. The attached gold nanoparticles show unique surface mobility on the silica core surface, which allows for nanoparticle rearrangement into a 2D ring pattern surrounding the silica nanoparticle template when the core–satellite structures are cast to a planar surface. When etching away the silica core under conditions in which the polymer shell fixes the satellites to the substrate, highly ordered ring-shaped patterns of gold nanoparticles are formed. By variation of the size of the parental particles – 13 to 28 nm for gold nanoparticles and 39 to 62 nm for silica nanoparticles – a great library of different ring-structures regarding size and particle number is accessible with relative ease. The proposed protocol is low-cost and can easily be scaled up. It moreover demonstrates the power of hydrogen bonds in polymers as a dynamic anchoring tool for creating nanoclusters with rearrangement ability. We believe that this concept constitutes a powerful strategy for the development of new and innovative nanostructures.

Magnetophoretic Control of Diamagnetic Particles Inside an Evaporating Droplet.

The present study reports the magnetophoresis of diamagnetic particles in an evaporating ferrofluid droplet. Both solid and ring magnet arrangements are used to investigate the effect of magnetic field distribution. The distance of the magnet from the droplet is varied to study the effect of magnetic field strength. The magnetic field distribution is computed using COMSOL multiphysics software. Magnetometer measurements have been carried out to validate the simulation results. The motion of particles and the drying pattern of evaporating ferrofluid droplets are visualized using the confocal microscopy technique. Both bright-field and fluorescence imagings have been carried out to observe the differential deposition of the fluorescent particle (microparticle) and magnetic nanoparticles in the absence and presence of a magnetic field. The velocity of diamagnetic particles as a function of magnetic field distribution and strength has been studied using the micro-PIV technique. In the absence of the magnetic field, a ring-shaped deposition pattern is observed. The mixture of microparticles (diamagnetic) and nanoparticles (magnetic) is deposited between the outer and inner edges of the ring. The diamagnetic particles occupy the inner and outer edges of the ring. Magnetic particles travel toward the higher magnetic field zone and diamagnetic particles move toward the smaller magnetic field zone when a magnetic field is applied by a solid magnet placed over the droplet. This can be attributed to the negative magnetic force originating from the difference between the susceptibility of magnetic and nonmagnetic particles. The negative magnetic force on the microparticle increases as the magnetic field intensity increases, causing the microparticle to convect faster toward the contact line. The deposition behavior can be reversed or suppressed using a ring magnet in place of a solid magnet. In this case, the negative magnetic force is stronger at the contact line region of the droplet and decreases as it approaches the center region of the droplet. The deposition behavior of diamagnetic particle depends on the balance between the Marangoni force and the magnetophoretic force. Overall, the present study demonstrates the capability of the controlled deposition of diamagnetic polystyrene particles by suitable arrangement of the solid and ring magnet.

Factors affecting the diameter of ring-shaped deposition patterns in inertial impactors having small S/W ratios

Inertial impactors are an important part of aerosol science where they are used to obtain particle size distributions in a wide range of practical and scientific applications. A shortcoming of thes...

Block Phenomena During Electric Micro-Stimulation of Pyramidal Cells and Retinal Ganglion Cells.

Electric micro-stimulation of the nervous system is a means to restore various body functions. The stimulus amplitude necessary to generate action potentials, the lower threshold (LT), is well characterized for many neuronal populations. However, electric overstimulation above an upper threshold (UT) prevents action potential generation and therefore hinders optimal neuro-rehabilitation. Previous studies demonstrated the impact of the UT in micro-stimulation of retinal ganglion cells (RGCs). The observed phenomenon is mostly explained by (i) reversed sodium ion flow in the soma membrane, and (ii) anodal surround block that hinders spike conduction in strongly hyperpolarized regions of the axon at high stimulus intensities. However, up to now, no detailed study of the nature of these phenomena has been presented, particularly for different cell types. Here, we present computational analyses of LT and UT for layer 5 pyramidal cells (PCs) as well as alpha RGCs. Model neurons were stimulated in close vicinity to the cell body and LTs and UTs as well as the ratio UT/LT were compared. Aside from a simple point source electrode and monophasic stimuli also realistic electrode and pulse configurations were examined. The analysis showed: (i) in RGCs, the soma contributed to action potential initiation and block for small electrode distances, whereas in PCs the soma played no role in LTs or UTs. (ii) In both cell types, action potential always initiated within the axon initial segment at LT. (iii) In contrast to a complete block of spike conductance at UT that occurred in RGCs, an incomplete block of spiking appeared in PC axon collaterals. (iv) PC axon collateral arrangement influenced UTs but had small impact on LTs. (v) Population responses of RGCs change from circular regions of activation to ring-shaped patterns for increasing stimulus amplitude. A better understanding of the stimulation window that can reliably activate target neurons will benefit the future development of neuroprostheses.

A deep learning approach for inverse design of the metasurface for dual-polarized waves

Compared to the conventional metasurface design, machine learning-based methods have recently created an inspiring platform for an inverse realization of the metasurfaces. Here, we have used the Deep Neural Network (DNN) for the generation of desired output unit cell structures in an ultra-wide working frequency band for both TE and TM polarized waves. To automatically generate metasurfaces in a wide range of working frequencies from 4 to 45 GHz, we deliberately design an 8 ring-shaped pattern in such a way that the unit-cells generated in the dataset can produce single or multiple notches in the desired working frequency band. Compared to the general approach, whereby the final metasurface structure may be formed by any randomly distributed "0" and "1", we propose here a restricted output structure. By restricting the output, the number of calculations will be reduced and the learning speed will be increased. Moreover, we have shown that the accuracy of the network reaches 91\%. Obtaining the final unit cell directly without any time-consuming optimization algorithms for both TE and TM polarized waves, and high average accuracy, promises an effective strategy for the metasurface design; thus, the designer is required only to focus on the design goal.

Fabrication of Ring-Shaped Deposits of Polystyrene Microparticles Driven by Thermocapillary Mechanism.

Fabrication of ring-shaped deposits of microparticles on solid surfaces with the desired length scales and morphology of particle arrangements is of great importance when developing modern optical and electronic resonators, chemical sensors, touch screens, field-emission displays, porous materials, and coatings with various functional properties. However, the controlled formation of ring-shaped patterns scaling from a few millimeters up to centimeters with simultaneous control of particle arrangement at the microscale is one of the most challenging problems in advanced materials science and technology. Here, we report a fabrication approach for ring-shaped structures of microparticles on a glass surface that relied on a local thermal impact produced by the subsurface heater and heat sink. Thermocapillary convection in the liquid covering microparticles in combination with evaporative lithography is responsible for the particle transport and the assembling into the ring-shaped patterns. An advantageous feature of this approach is based on the control of thermocapillary flow direction, achieved by changing the sign of the temperature gradient in the liquid, switching between heating and cooling modes. That allows for changing the particle transfer direction to create the ring-shaped deposits and dynamically tune their size and density distribution. We have studied the influence of the power applied to the heat source/sink and the duration of the applied thermal field on the rate of the ring fabrication, the sizes of the ring and the profile of the particle distribution in the ring. The proposed method is flexible to control simultaneously the centimeter scale and microscale processes of transfer and arrangements of particles and can be applied to the fabrication of ring structures of particles of different nature and shape.

Exact Scan Patterns of Rotational Risley Prisms Obtained with a Graphical Method: Multi-Parameter Analysis and Design

Rotational Risley prisms are one of the fastest two-dimensional (2D) optomechanical scanning systems. Their drawback is the strong non-linearity of the scan patterns they produce, in contrast to the most common (but slower) raster scanning modalities of 2D dual axis galvanometer scanners (GSs) or Micro-Electro-Mechanical Systems (MEMS) with oscillatory mirrors. The aim of this work is to develop a graphical method, which, to our knowledge, we have introduced to determine and characterize, using a commercially-available mechanical design program (for example CATIA V5R20 (Dassault Systems, Paris, France)) to simulate the exact scan patterns of rotational Risley prisms. Both the maximum and minimum angular and linear deviations of this type of scanner are deduced theoretically to characterize the outer diameter/Field-of-View (FOV) and the inner diameter (of the blind zone) of its ring-shaped patterns, respectively. This multi-parameter analysis is performed in correlation with the shape of the scan patterns, considering the four possible configurations of laser scanners with a pair of rotational Risley prisms, as well as all their parameters: prisms angles, refractive indexes, rotational speeds, distance between the two prisms, and the distance from the system to the scanned plane. Marshall’s synthetic parameters are also considered, i.e., the ratios of the rotational velocities and of the angles of the prisms. Rules-of-thumb for designing this optomechanical scanner are extracted from this analysis, regarding both shapes and dimensions of the scan patterns to be produced. An example of experimental validation completes the mathematical analysis and the performed simulations.

Dissecting subcycle interference in photoelectron holography

Multipath holographic interference in strong-field quantum tunnel ionization is key to revealing sub-Angstrom attosecond dynamics for molecular movies. This critical sub-cycle motion is often obscured by longer time-scale effects such as ring-shaped patterns that appear in above-threshold ionization (ATI). In the present work, we overcome this problem by combining two novel techniques in theory and experimental analysis: unit-cell averaging and time-filtering data and simulations. Together these suppress ATI rings and enable an unprecedented highly-detailed quantitative match between strong-field ionization experiments in argon and the Coulomb-quantum orbit strong-field approximation (CQSFA) theory. Velocity map images reveal fine modulations on the holographic spider-like interference fringes that form near the polarization axis. CQSFA theory traces this to the interference of three types of electron pathways. The level of agreement between experiment and theory allows sensitive determination of quantum phase differences and symmetries, providing an important tool for quantitative dynamical imaging in quantum systems.

Propagation dynamics of radially polarized symmetric Airy beams in the fractional Schrödinger equation

We analyze the propagation dynamics of radially polarized symmetric Airy beams (R-SABs) in a (2+1)-dimensional optical system with fractional diffraction, modeled by the fractional Schrödinger equation (FSE) characterized by the Lévy index, α. The autofocusing effect featured by such beams becomes stronger, while the focal length becomes shorter, with the increase of α. The effect of the intrinsic vorticity on the autofocusing dynamics of the beams is considered too. Then, the ability of R-SABs to capture nano-particles by means of radiation forces is explored, and multiple capture positions emerging in the course of the propagation are identified. Finally, we find that the propagation of the vortical R-SABs with an off-axis shift leads to rupture of the ring-shaped pattern of the power-density distribution.

Ring distribution patterns-diversification or speciation? Comparative phylogeography of two small mammals in the mountains surrounding the Sichuan Basin.

Studying the genetic differentiation in a unique geographical area contributes to understanding the process of speciation. Here, we explore the spatial genetic structure and underlying formation mechanism of two congeneric small mammal species (Apodemus draco and A.chevrieri), which are mainly distributed in the mountains surrounding the lowland Sichuan Basin, southwest China. We applied a set of comparative phylogeographical analyses to determine their genetic diversification patterns, combining mitochondrial (Cytb and COI) and nuclear (microsatellite loci) markers, with dense sampling throughout the range (411 A.draco from 21 sites and 191 A.chevrieri from 22 sites). Moreover, we performed three complementary statistical methods to investigate the correlation between genotype and geographical and environmental components, and predicted the potential suitable distributional range under the present and historical climate conditions. Our results suggest that both species have experienced allopatric differentiation and admixture in historical periods, resulting in a ring-shape diversification, under the barrier effect of the Sichuan Basin. We infer that the tectonic events of the Qinghai-Tibetan Plateau and climatic oscillations during the Quaternary played an important role on the genetic divergence of the two species by providing environmental heterogeneity and geographical variation. Our study reveals a case of two sympatric small mammals following a ring-shaped diversification pattern and provides insight into the process of differentiation.

Deep neural network-based automatic metasurface design with a wide frequency range

Beyond the scope of conventional metasurface, which necessitates plenty of computational resources and time, an inverse design approach using machine learning algorithms promises an effective way for metasurface design. In this paper, benefiting from Deep Neural Network (DNN), an inverse design procedure of a metasurface in an ultra-wide working frequency band is presented in which the output unit cell structure can be directly computed by a specified design target. To reach the highest working frequency for training the DNN, we consider 8 ring-shaped patterns to generate resonant notches at a wide range of working frequencies from 4 to 45 GHz. We propose two network architectures. In one architecture, we restrict the output of the DNN, so the network can only generate the metasurface structure from the input of 8 ring-shaped patterns. This approach drastically reduces the computational time, while keeping the network’s accuracy above 91%. We show that our model based on DNN can satisfactorily generate the output metasurface structure with an average accuracy of over 90% in both network architectures. Determination of the metasurface structure directly without time-consuming optimization procedures, an ultra-wide working frequency, and high average accuracy equip an inspiring platform for engineering projects without the need for complex electromagnetic theory.

Exploring HNC and HCN line emission as probes of the protoplanetary disk temperature

Context. The distributions and abundances of molecules in protoplanetary disks are powerful tracers of the physical and chemical disk structures. The abundance ratios of HCN and its isomer HNC are known to be sensitive to gas temperature. Their line ratios might therefore offer a unique opportunity to probe the properties of the emitting gas. Aims. We investigate the HNC and HCN line emission in disks at (sub-)millimeter wavelengths and explore their potential utility for probing disk temperature and other disk properties. Methods. Using the 2D thermochemical code DALI, we ran a set of disk models accounting for different stellar properties and radial and vertical disk structures, with an updated chemical network for the nitrogen chemistry. These modeling results were then compared with observations, including new observations obtained with the Atacama Large Millimeter/submillimeter Array (ALMA) of HNC J = 3−2 for the TW Hya disk and HNC J = 1−0 for 29 disks in Lupus. Results. Similar to CN, HCN and HNC have brighter line emission in models with larger disk flaring angles and higher UV fluxes. HNC and HCN are predicted to be abundant in the warm surface layer and outer midplane region, which results in ring-shaped emission patterns. However, the precise emitting regions and emission morphology depend on the probed transition, as well as on other parameters such as C and O abundances. The modeled HNC-to-HCN line intensity ratio increases from <0.1 in the inner disk to up to 0.8 in the outer disk regions, which can be explained by efficient HNC destruction at high temperatures. Disk-integrated HNC line fluxes from current scarce observations and its radial distribution in the TW Hya disk are broadly consistent with our model predictions. Conclusions. The HNC-to-HCN flux ratio robustly increases with radius (decreasing temperature), but its use as a chemical thermometer in disks is affected by other factors, including UV flux and C and O abundances. High-spatial resolution ALMA disk observations of HNC and HCN that can locate the emitting layers would have the great potential to constrain both the disk thermal and UV radiation structures, and also to verify our understanding of the nitrogen chemistry.

Erosion behavior of aluminum by an inclined cavitating jet

The erosion behavior of pure aluminum by an inclined cavitating jet in the acceleration period was experimentally investigated for potential applications in peening and cleaning. The mass loss and surface topography of the eroded specimens were investigated to unravel the erosion mechanism under inclined impingement with various feeding pressure. The cavitation cloud motion was numerically investigated in various erosion stages with two-phase CFD calculation. Results show that at the first optimum standoff distance of mass loss l1 close to the nozzle bottom, erosion occurs in two ring-like areas, whereas the erosion at the downstream second optimum standoff distance l2 appears as a single ring at inclined angles αi∈ [0∘, 15∘] and as separated crescent-shaped areas at αi = 30∘ and 45∘. The increasing αi induces a higher cumulative erosion ratio in the acceleration period at Pin = 15 MPa and 13 MPa, whereas the aggressive ability of inclined impingement is attenuated at Pin = 11 MPa due to the low cavitation intensity. An obvious transition from plastic deformation to erosion damage is found on the boundary of the main erosion area. Under the inclined impingement, the upper side of the main erosion area shows much more severe erosion damage than the lower side due to the blocking effect, where the upward spread of the cavitation clouds is blocked and their collapse primarily occurs in the erosion valley. The erosion develops with the a ring-shaped pattern at αi∈ [0∘, 5∘]. At higher inclined impingement angles αi∈ [15∘, 45∘], the erosion primarily develops along the surface normal direction in the part of the main erosion area closer to the nozzle exit.

Comparative Analysis of Gas–Solid–Liquid Coupling Behavior in Front of the Working Face Before and After Water Injection During Coal Mining

Coal seam water injection is a critical measure for the prevention of coal-and-gas outbursts. However, the gas–solid–liquid coupling effect in front of the working face before and after water injection during coal mining is scarcely investigated. This issue was explored in this study by (a) numerical simulation of stress, gas pressure, and water saturability distribution as well as their coupling effect in front of the working face via the proposed multi-field coupling model run in the COMSOL software and (b) comparative analysis of the above parameters before and after water injection. During coal mining, stress in front of the working face first increased and then dropped to the initial value as the distance from the coal wall increased, while gas pressure exhibited a similar distribution pattern. After water injection, the stress distribution pattern remained almost unchanged, but the stress peak shifted forward, the stress relief zone broadened, while the gas pressure around the borehole dropped. In particular, the gas pressure in front of the borehole increased slightly at the beginning of water injection. As water was permanently injected, the gas pressure in front of the borehole decreased, while that around the borehole further decreased. Meanwhile, the water saturability of the coal seam around each borehole increased, with a ring-shaped distribution pattern. Due to the interaction of water injection among multiple boreholes, the contour lines of water saturability showed semi-circular distribution patterns around multiple boreholes.

EXPERIMENTAL STUDY OF THE TRANSFER OF MICROPARTICLES IN A THIN LIQUID LAYER UNDER THE INFLUENCE OF A TEMPERATURE GRADIENT

This article studies the main regularities of polyethylene microparticles transfer process in a layer of volatile and non-volatile fluid by thermocapillary currents under local heating and cooling. The authors show the possibility of creating circular and ring-shaped patterns by inducing positive and negative radial-directional temperature gradients. A methodology and computer program have been developed to quantify the transfer process, consisting in measuring the area of the particle pattern (assembly) formed during heating and the area freed from the particles (cleaning area) during cooling on a sequence of video recording frames obtained with an optical microscope. This technique is based on comparing the intensity of image pixels with respect to a threshold value and counting the total area of pixels occupied or not occupied by particles. The influence of such experiment parameters as the volume of the carrier fluid (layer thickness), at a constant number of particles, fluid evaporation and the ratio of particle and fluid densities on the size of the resulting pattern and the time of reaching the steady state has been established. The results show that the area of the final pattern during local heating and the clearing area, during local cooling, tends to decrease with increasing layer thickness, while the time of reaching the steady state does not depend on the layer thickness, but depends on the properties of the liquid and the ratio of particle and liquid densities.

Irradiation-induced segregation at dislocation loops in CoCrFeMnNi high entropy alloy

To understand the redistribution of alloying elements in high entropy alloys under irradiation, a CoCrFeMnNi alloy was irradiated with 1 MeV Kr ions at room temperature and at 500°C, and characterized with atom probe tomography and transmission electron microscopy. At 500°C, Co and Ni were enriched around the interstitial, faulted and perfect, dislocation loops resulted from the ion irradiation. In contrast, no segregation was observed at room temperature. The inverse Kirkendall effect through vacancy flux, as opposed to the interstitial binding mechanism, was the primary underlying process attributing to the observed segregation. In addition, a ring-shaped segregation pattern was observed at the faulted dislocation loops, indicating a non-equilibrium nature of the defect clustering and solute segregation process in CoCrFeMnNi under irradiation at high temperature.