Cadmium In Rice Research Articles

Effects of alternate wetting and drying on oxyanion-forming and cationic trace elements in rice paddy soils: impacts on arsenic, cadmium, and micronutrients in rice.

Rice is a global dietary staple and its traditional cultivation under flooded soil conditions leads to accumulation of arsenic (As) in rice grains. Alternate wetting and drying (AWD) is a widely advocated water management practice to achieve lower As concentrations in rice, water savings, and decreased methane emissions. It is not yet clear whether AWD leads to tradeoffs between concentrations of As and micronutrient elements (e.g., zinc, manganese, molybdenum) in rice grain. We analyzed pore water chemistry and rice grain composition data from a field experiment conducted in Arkansas, USA, in 2017 and 2018 to test the hypothesis that AWD will have diverging effects on oxyanion-forming (arsenic, molybdenum) vs. cationic (cadmium, zinc, manganese, copper) trace elements. This was hypothesized to occur via decreases in soil pH and/or precipitation of iron oxide minerals during oxidizing conditions under AWD. Solubility of all trace elements, except zinc, increased in more reducing conditions. Consistent with our hypothesis, AWD tended to increase grain concentrations of cationic elements while decreasing grain concentrations of oxyanionic elements. Decreases in total As in rice grains under AWD were mainly driven by changes in dimethylarsinic concentrations, with negligible changes in inorganic As. Linear mixed-effects modeling showed that effects of AWD on grain composition were more significant in 2017 compared to 2018. These differences may be related to the timing of dry-downs in the developmental stage of rice plants, with dry-downs during the heading stage of rice development leading to larger impacts on grain composition of certain elements. We also observed significant interannual variability in grain elemental composition from continuously-flooded fields and postulate the warmer temperatures in 2018 may have played a role in these differences.

Mitigation strategies for excessive cadmium in rice.

Cadmium (Cd)-contaminated rice is a human food safety problem that lacks a clear solution. A large amount of rice having an excessive Cd content is processed yearly, but it cannot be discarded and placed in landfills because it will cause secondary pollution. How do we best cope with this toxic rice? From the perspectives of food safety, food waste prevention, and human hunger eradication, the use of contemporary physical, chemical, and biological techniques to lower the Cd content in postharvest Cd-contaminated rice so that it can be used safely is the best course of action. In this review, the contamination, chemical speciation, and distribution of Cd in rice are analyzed and discussed, as are the methods of Cd removal from rice, including a comparison of the advantages and disadvantages of various techniques. Owing to the limitations of current technology, research and technological development recommendations for removing Cd from rice grain are presented. The chemical and biological methods produce higher Cd-removal rates than physical methods. However, they are limited to small-scale laboratory applications and cannot be applied on a large industrial scale. For the efficient safe removal of Cd from food, mixed fermentation with lactic acid bacteria and yeast has good application prospects. However, limited strains having high Cd-removal rates have been screened. In addition, modern biotechnology has rarely been applied to reduce rice Cd levels. Therefore, applying genetic engineering techniques to rapidly obtain microorganisms with high Cd-removal rates in rice should be the focus of future research.

A combined strategy to mitigate the accumulation of arsenic and cadmium in rice (Oryza sativa L.)

Arsenic and cadmium in rice grain are of growing concern in the global food supply chain. Paradoxically, the two elements have contrasting behaviors in soils, making it difficult to develop a strategy that can concurrently reduce their uptake and accumulation by rice plant. This study examined the combined impacts of watering (irrigation) schemes, different fertilizers and microbial populations on the bioaccumulation of arsenic and cadmium by rice as well as on rice grain yield. Compared to drain-flood and flood-drain treatments, continuously flooded condition significantly reduced the accumulation of cadmium in rice plant but the level of arsenic in rice grain remained above 0.2 mg/kg, which exceeded the China national food safety standard. Application of different fertilizers under continuously flooded condition showed that compared to inorganic fertilizer and biochar, manure addition effectively reduced the accumulation of arsenic over three to four times in rice grain and both elements were below the food safety standard (0.2 mg/kg) while significantly increasing the rice yield. Soil Eh was the critical factor in the bioavailability of cadmium, while the behavior of arsenic in rhizosphere was associated with the iron cycle. The results of the multi-parametric experiments can be used as a roadmap for low-cost and in-situ approach for producing safe rice without compromising the yield.

Metallochaperone protein OsHIPP17 regulates the absorption and translocation of cadmium in rice (Oryza sativa L.)

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in regulating heavy metal responding activities in plants. Yet only a handful of studies have characterized the functions of HIPPs. In this study, a novel HIPP member OsHIPP17 was functionally characterized, which was involved in the tolerance of yeast and plants to cadmium (Cd). The Cd accumulation in yeast cells was increased due to the overexpression of OsHIPP17. Nevertheless, the overexpression of OsHIPP17 in Arabidopsis thaliana resulted in compromised growth under Cd stress. Meanwhile, the mutation of OsHIPP17 resulted in 38.9–40.9 % increase of Cd concentration in rice roots as well as 14.3–20.0 % decrease of Cd translocation factor. Further investigation of the genes responsible for Cd absorption and transporter indicated that the expression levels of these genes were also perturbed. In addition, two OsHIPP17-interacting proteins, OsHIPP24 and OsLOL3 were identified in a yeast two hybrid assay. Further analysis of their functions revealed that OsHIPP24 or OsLOL3 may be involved in the regulation of Cd tolerance by OsHIPP17 in rice. All above results implied that OsHIPP17 may affect Cd resistance by regulating the absorption and translocation of Cd in rice.

Cadmium, arsenic, and mineral nutrients in rice and potential risks for human health in South China.

Rice (Oryza sativa L.) is one of the most important staple food crops worldwide. For people fed on rice, toxic elements cadmium (Cd) and arsenic (As) and mineral nutrients in rice are pivotal to evaluate potential risks of harmful element intake and malnutrition. We collected rice samples of 208 cultivars (83 inbred and 125 hybrid) from fields in South China and determined Cd, As, As species, and mineral elements in brown rice. Chemical analysis shows that the average content of Cd and As in brown rice were 0.26 ± 0.32 and 0.21 ± 0.08mg·kg-1, respectively. Inorganic As (iAs) was the dominative As species in rice. Rice Cd and iAs in 35.1% and 52.4% of the 208 cultivars exceeded rice Cd and iAs limits, respectively. Significant variations of rice subspecies and regions were found for Cd, As, and mineral nutrients in rice (P < 0.05). Inbred rice had lower As uptake and more balanced mineral nutrition than hybrid species. Significant correlation was observed between Cd, As versus mineral elements like Ca, Zn, B, and Mo (P < 0.05). Health risk assessment indicates that high risks of non-carcinogenic and carcinogenic of Cd and iAs, and malnutrition, in particular Ca, protein and Fe deficiencies, might be caused by rice consumption in South China.

Cadmium Absorption in Various Genotypes of Rice under Cadmium Stress.

Cadmium (Cd) is a kind of heavy metal. Cadmium pollution in paddy fields will accumulate a large amount of cadmium in rice, which will affect the growth and development of rice. In addition, long-term consumption of rice contaminated with Cd can harm human health. In this study, four rice varieties with high Cd accumulation (S4699, TLY619, JHY1586, QLY155) and four varieties with low Cd accumulation (YY4949, CYJ-7, G8YXSM, MXZ-2) were screened through field experiments for two consecutive years (2021 and 2022) and differences in antioxidant enzyme systems and expression of genes in their organs were analyzed. The total Cd content showed as follows: indica rice > japonica rice, high-Cd-accumulation variety > low-Cd-accumulation variety, and the total Cd content of each organ of rice showed root > stem > leaf > grain. The results of the antioxidant enzyme system showed that the contents of malondialdehyde (MAD), reduced glutathione (GSH), oxidized glutathione (GSSH), and peroxidase (POD) were positively correlated with the total Cd content in rice, and superoxide dismutase (SOD) showed the opposite performance in the leaves. There was no correlation between catalase (CAT) and Cd content, but CAT content decreased in leaves and grains and increased in roots and stems with increasing fertility. Based on this study, RT-qPCR was used to further validate the expression of Cd-uptake-related genes in different rice varieties. It was found that high expression of OsHMA3, OsCCX2, OsNRAMP5, and OsHMA9 genes promoted Cd uptake and translocation in rice, especially in rice varieties with high Cd accumulation. The high expression of OslRT1, OsPCR1, and OsMTP1 genes hindered Cd uptake by rice plants, which was especially evident in low-accumulating Cd rice varieties. These results provide an important theoretical reference and scientific basis for our in-depth study and understanding of the mechanism of cadmium stress tolerance in rice.

Significant difference in the efficacies of silicon application regimes on cadmium species and environmental risks in rice rhizosphere

Silicon (Si) is commonly applied as base-fertilizer or foliar-topdressing to palliate the uptake-translocation-accumulation of cadmium (Cd) in rice through Si–Cd antagonism. However, little is known about the fate of Cd in rice rhizosphere soil and its eco-environmental effects under different Si treatments. Here, systematic works had been carried out to elucidate the Cd species, soil properties, and environmental risks in rice rhizosphere driven by different Si soil-fertilization regimes including CK (without Si-addition), TSi (added before transplanting stage), JSi (added at jointing stage), and TJSi (split into two equal parts, added half before transplanting and another half at jointing stage). Results showed that TJSi outperformed the rest of fertilization regimes. The solid-phase-Cd concentrations treated with TSi, TJSi and JSi were increased by 4.18%, 5.73% and 3.41%, respectively, when compared to CK. The labile Cd (F1+F2) proportion of TJSi was reduced by 16.30%, 9.30% and 6.78%, respectively, when compared to CK, TSi, and JSi. Simultaneously, the liquid-phase-Cd concentration was appreciably suppressed by TJSi throughout the rice lifecycle, while TSi mainly abated Cd dissociation during the vegetative period, and JSi attenuated it during the grain-filling stage. The mobility factor of Cd treated with TJSi was the lowest, which was significantly lower than that of TSi (9.30%) and JSi (6.78%), respectively. Similarly, the oral exposure risk of TJSi was reduced by 4.43% and 32.53%; and the food-chain exposure risk of TJSi was decreased by 13.03% and 42.78%. Additionally, TJSi was the most effective in promoting enzyme activities and nutrient content in rhizosphere soil. Overall, TJSi is more positive and sustainable than TSi and JSi in reconstructing Cd-contaminated rhizosphere environments and abating the environmental risks of Cd. Agronomic practices in Cd-contaminated paddy soils can be informed by applying Si-fertilizer separately before transplanting and at jointing stage to achieve soil welfare and food security.

Synergistic Effects of Water Management and Silicon Foliar Spraying on the Uptake and Transport Efficiency of Cadmium in Rice (Oryza sativa L.)

To study the synergistic effects of water management and silicon (Si) foliar spraying on the uptake and transport of cadmium (Cd) in rice, we designed four treatments: conventional intermittent flooding + no Si foliar spraying (CK), continuous flooding throughout the growth stage + no Si foliar spraying (W), conventional intermittent flooding + Si foliar spraying (Si) and continuous flooding throughout the growth stage + Si foliar spraying (WSi). The results show that WSi treatment reduced the uptake and translocation of Cd by rice and significantly reduced the brown rice Cd content, with no effect on rice yield. Compared with CK, the Si treatment increased the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of rice by 6.5-9.4%, 10.0-16.6% and 2.1-16.8%, respectively. The W treatment decreased these parameters by 20.5-27.9%, 8.6-26.8% and 13.3-23.3%, respectively, and the WSi treatment decreased them by 13.1-21.2%, 3.7-22.3% and 2.2-13.7%, respectively. The superoxide dismutase (SOD) and peroxidase (POD) activity decreased by 6.7-20.6% and 6.5-9.5%, respectively, following the W treatment. Following the Si treatment, SOD and POD activity increased by 10.2-41.1% and 9.3-25.1%, respectively, and following the WSi treatment, they increased by 6.5-18.1% and 2.6-22.4%, respectively. Si foliar spraying ameliorated the detrimental effects of continuous flooding throughout the growth stage on photosynthesis and antioxidant enzyme activity. We conclude that synergistic continuous flooding throughout the growth stage, combined with Si foliar spraying, can significantly block Cd uptake and translocation and is therefore an effective means of reducing the accumulation of Cd in brown rice.

Metallochaperone OsHIPP9 is involved in the retention of cadmium and copper in rice.

Metallochaperones are a unique class of proteins that play crucial roles in metal homoeostasis and detoxification. However, few metallochaperones have been functionally characterised in rice. Heterologous expression of Heavy metal-associated Isoprenylated Plant Protein 9 (OsHIPP9), a metallochaperone, altered yeast tolerance to cadmium (Cd) and copper (Cu). We investigated the physiological role of OsHIPP9 in rice. OsHIPP9 was primarily expressed in the root exodermis and xylem region of enlarged vascular bundles (EVB) at nodes. KO of OsHIPP9 increased the Cd concentrations of the upper nodes and panicle, but decreased Cd in expanded leaves. KO of OsHIPP9 decreased Cu uptake and accumulation in rice. Constitutive OX of OsHIPP9 increased Cd and Cu accumulation in aboveground tissues and brown rice. OsHIPP9 showed binding capacity for Cd and Cu. We propose that OsHIPP9 has dual metallochaperone roles, chelating Cd in the xylem region of EVB for Cd retention in the nodes and chelating Cu in rice roots to aid Cu uptake.

Review of Cadmium Pollution in Preboiled-Rice

Based on the preboiled-rice processing technology, the distribution and form of cadmium in rice seeds were analyzed and summarized. The origin and development of preboiled-rice and its processing technology were elaborated in detail. The characteristics and hazards of heavy metal cadmium, the existence and distribution of cadmium in rice, the detection of cadmium content in rice seeds and its processing and reduction technology were discussed scientifically and systematically. To explore the possibility of processing rice contaminated with cadmium into usable rice. To reduce a certain degree of metal cadmium and improve the use quality of preboiled-rice, and to provide a reference for the process of rice soaking cadmium in the process of preboiled-rice processing, to expand the ideas for the rational use of cadmium contaminated rice,hoping to greatly reduce the waste of food, and at the same time to play a certain guiding role in the development of rice processing industry.

Determinations of Cadmium, Tin, Copper, Iron and Zinc in Food Samples by Electrothermal and Flame Atomic Absorption Spectrometries

Cadmium, tin, iron, copper, and zinc in tea, rice, and liver samples, and standard reference materials (IRMM 804 Rice Flour, GBW 10010 Rice, Bovine Liver1577c and GBW 07605 Tea Leaves) were determined by using electrothermal and flame atomic absorption spectrometries. Cd and Sn in sample solutions were determined with electrothermal atomic absorption spectrometry by using palladium (Pd) as a matrix modifier. Pyrolysis and atomization temperatures and pyrolysis times of Cd and Sn in sample solutions were optimized in the absence or presence of Pd. Pyrolysis temperatures of Cd and Sn found in the presence of Pd were 800°C and 1200°C, respectively. Iron, Cu, and Zn in samples were determined by flame atomic absorption spectrometry. The accuracy and precision of the method were validated by determining elements in standard reference materials. Limits of detection for Sn, Cd, Fe, Cu, and Zn were found as 0.66, 0.26, 2.0, 1.9, and 1.2 μg/L, respectively. Concentrations of elements found in samples were compared with maximum values reported by World Health Organization (WHO) and other studies.

Relative Bioavailability of Cadmium in Rice: Assessment, Modeling, and Application for Risk Assessment.

Rice consumption is the primary route of cadmium (Cd) exposure to the populations with rice as the staple food. To accurately assess the potential health risks of Cd exposure via rice consumption, determination of Cd relative bioavailability (RBA) in rice is necessary. However, large variations exist in Cd-RBA, hindering the application of source-specific Cd-RBA values to different rice samples. In this study, we collected 14 rice samples from Cd contaminated areas and determined both rice compositions and Cd-RBA using in vivo mouse bioassay. Total Cd concentration varied from 0.19 to 2.54 mg/kg in the 14 rice samples, while Cd-RBA in rice ranged from 42.10% to 76.29%. Cadmium-RBA in rice correlated positively with calcium (Ca) (R = 0.76) and amylose content (R = 0.75) but negatively with the concentrations of sulfur (R = -0.85), phosphorus (R = -0.73), phytic acid (R = -0.68), and crude protein (R = -0.53). Cd-RBA in rice can be predicted by Ca and phytic acid concentrations in a regression model (R2 = 0.80). Based on the total and bioavailable Cd concentrations in rice, weekly dietary Cd intake for adults was estimated to be 4.84-64.88 and 2.04-42.29 μg/kg bw/week, respectively. This work demonstrates the possibility of Cd-RBA prediction based on rice compositions and provides valuable suggestions for health risk assessment with consideration of Cd-RBA.

Convolutional graph neural networks-based research on estimating heavy metal concentrations in a soil-rice system.

Estimating heavy metal concentrations in soil-rice systems is of great significance to identify the factors controlling heavy metal transfer in soil-crop ecosystems. Recent research utilizes the advantage of convolutional calculations to extract and learn complicated information from 17 environmental covariates in rice and achieve promising results. However, as the complexity and interconnectivity in soil-crop ecosystem, just relying on convolutional calculations and a deep network structure is far from enough. The data processed by traditional deep learning technologies even with convolutional calculations are limited to Euclidean space; these architectures do not have the ability to extract information from the relationships in graph structures, which may contain rich information. Thus, in this paper, we try to integrate graph information into convolutional calculations for heavy metal prediction and propose a model named ConvGNN-HM. ConvGNN-HM combines the advantages of graph learning and convolutional calculations to predict heavy metal concentrations in a soil-rice system with analysis of 17 environmental factors. For comparison, we conduct an experiment to compare ConvGNN-HM with techniques with convolutional neural networks, multilayer perceptron, back-propagation neural networks, support vector machine, random forest, Bayesian ridge regression, and multiple linear regression. The experimental results illustrate that ConvGNN-HM got the best prediction values; the R2 values of ConvGNN-HM for cadmium (Cd), plumbum (Pb), chromium (Cr), arsenic (As), and hydrargyrum (Hg) in rice were 0.84, 0.75, 0.79, 0.49, and 0.83, respectively, and the MAE values were also acceptable. We further conduct sensitivity analysis to demonstrate the stability and robustness of ConvGNN-HM. This study demonstrates the usefulness of combining graph learning and convolutional calculations in the prediction of heavy metal concentrations and provides a new perspective to build multidimensional and multi-scale complex ecosystem models.

Low levels of arsenic and cadmium in rice grown in southern Florida Histosols - Impacts of water management and soil thickness

Rice is planted as a rotation crop in the sugarcane-dominant Everglades Agricultural Area (EAA) in southern Florida. The Histosols in this area are unlike other mineral soils used to grow rice due to the high organic content and land subsidence caused by rapid oxidation of organic matter upon drainage. It remains unknown if such soils pose a risk of arsenic (As) or cadmium (Cd) mobilization and uptake into rice grain. Both As and Cd are carcinogenic trace elements of concern in rice, and it is important to understand their soil-plant transfer into rice, a staple food of global importance. Here, a mesocosm pot study was conducted using two thicknesses of local soil, deep (D, 50 cm) and shallow (S, 25 cm), under three water managements, conventional flooding (FL), low water table (LWT), and alternating wetting and drying (AWD). Rice was grown to maturity and plant levels of As and Cd were determined. Regardless of treatments, rice grown in these Florida Histolsols has very low Cd concentrations in polished grain (1.5–5.6 μg kg−1) and relatively low total As (35–150 μg kg−1) and inorganic As (35–87 μg kg−1) concentrations in polished grain, which are below regulatory limits. This may be due to the low soil As and Cd levels, high soil cation exchange capacity due to high soil organic matter content, and slightly alkaline soil pH. Grain As was significantly affected by water management (AWD < FL = LWT) and its interaction effect with soil thickness (AWD-D ≤ AWD-S ≤ FL-D = LWT-S = LWT-D ≤ FL-S), resulting in as much as 62 % difference among treatments. Grain Cd was significantly affected by water management (AWD > FL > LWT) without any soil thickness impact. In conclusion, even though water management has more of an impact on rice As and Cd than soil thickness, the low concentrations of As and Cd in rice pose little health risk for consumers.

Effect of Leaf Surface Regulation of Zinc Fertilizer on Absorption of Cadmium, Plumbum and Zinc in Rice (Oryza sativa L.)

The accumulation of heavy metals in rice is bound to affect human health and safety. In order to ensure food security, this study explores the effect of leaf surface regulation of zinc (Zn) fertilizer on the safety of rice in cadmium (Cd)-plumbum (Pb) polluted farmland. Through field experiments, the leaf surface control treatment of zinc fertilizer was set up, and the effects of leaf surface control of zinc fertilizer on rice yield, Cd and Pb concentration in different parts and zinc, nitrogen, phosphorus and potassium concentration in brown rice were studied in the growing period of rice. The results showed as follows: (1) Spraying twice or more in the growth stage of rice could increase the yield by 6.77–7.29% compared with the blank, which was significantly higher than that of single spraying in a certain growth stage. (2) After spraying zinc fertilizer on the leaf surface, Cd and Pb concentration in brown rice decreased by 29.52–56.01% and 11.10–28.34%, respectively, compared with CK. Two or more times of spraying can make Cd concentration in brown rice meet the Chinese standard GB 2762-2022, and one time of spraying can make Pb concentration in brown rice meet the standard. (3) Leaf surface control of zinc fertilizer could reduce the Cd enrichment ability of rice plant parts, and the Cd enrichment coefficient of brown rice was significantly reduced by 28.18–55.02%. Leaf surface control of zinc fertilizer can reduce Cd and Pb concentration in brown rice by reducing the transport ability of heavy metal Cd and Pb in rice roots to straw and then to brown rice. (4) The zinc concentration in brown rice was 18.16–20.68 mg·kg−1, which was 18.21–34.64% higher than that in CK, and the zinc enrichment effect was the most significant after spraying three times. Meanwhile, the nitrogen, phosphorus and potassium concentration in brown rice also increased with the increase of spraying times. By comprehensive analysis, the leaf surface control of zinc fertilizer can reduce the Cd and Pb concentration in rice, and the Cd and Pb concentration in brown rice can meet the Chinese standard GB 2762-2022 by spraying twice. At the same time, it can improve the concentration of zinc, nitrogen, phosphorus and potassium in brown rice, is feasible and has high economic benefits.

Highly catalytic nanoenzyme of covalent organic framework loaded starch- surface-enhanced Raman scattering/absorption bi-mode peptide as biosensor for ultratrace determination of cadmium.

High affinity peptides (PTs) have been used in nanoanalysis, but there are no reports which combine PTs with a liquid crystal (LC) covalent organic framework (COF) supported soluble starch (SS) catalytic amplification system as a biosensor recognition element. In this study, a new, highly sensitive and selective bi-mode molecular biosensor has been developed for the determination of cadmium ion (Cd2+). Specifically, a highly catalytic and stable COF supported SS nanosol catalyst was fabricated such that a nanocatalytic indicator reaction system for HAuCl4-sodium formate was established based on surface-enhanced Raman scattering (SERS). The Au nanoparticles produced exhibited a surface plasmon resonance (SPR) absorption peak at 535 nm and a SERS peak at 1,615 cm-1. Combining the nanocatalytic amplification indicator system with the specific PTs reaction permitted a sensitive and selective SERS/absorption bi-mode platform to be developed for the determination of cadmium in rice. The linear range for SERS determination was 0.025-0.95 nmol/L and the detection limit (DL) was 0.012 nmol/L.

The Status and Research Progress of Cadmium Pollution in Rice- (Oryza sativa L.) and Wheat- (Triticum aestivum L.) Cropping Systems in China: A Critical Review.

The accumulation of cadmium in rice (Oryza sativa L.) and wheat (Triticum aestivum L.) is a serious threat to the safe use of farmland and to the health of the human diet that has attracted extensive attention from researchers. In this review, a bibliometric analysis was performed using a VOS viewer (1.6.18, Netherlands) to investigate the status of cadmium contamination in rice and wheat growing systems, human health risks, mechanisms of Cd uptake and transport, and the corresponding research hotspots. It has a certain reference value for the prevention and control of cadmium pollution in rice and wheat planting systems in China and abroad. The results showed that the Cd content in rice and wheat planting systems in the Yangtze River Basin was significantly higher than that in other areas of China, and the Cd content in rice and wheat grains and the hazard quotient (HQ) in Hunan Province was the highest. The average Cd concentration exceeded the recommended limit by about 62% for rice and 81% for wheat. The main reasons for the high Cd pollution in rice and wheat growing areas in Hunan are mining activities, phosphate fertilizer application, sewage irrigation, and electronic equipment manufacturing. In this review, we demonstrate that cadmium toxicity reduces the uptake and transport of essential elements in rice and wheat. Cadmium stress seriously affected the growth and morphology of plant roots. In the shoots, Cd toxicity was manifested by a series of physiological injuries, such as decreased photosynthesis, soluble protein, sugar, and antioxidant enzyme activity. Cadmium that accumulates in the shoots is transferred to grains and then passes up the food chain to people and animals. Therefore, methods for reducing cadmium content in grains of rice and wheat are urgently needed, especially in Cd-contaminated soil. Current research on Cd pollution in rice and wheat planting systems focuses on the bioavailability of Cd, soil rhizosphere changes in wheat and rice, and the role of antioxidant enzyme systems in alleviating heavy metal stress in rice and wheat.

The lead and cadmium content in rice and risk to human health in China: A systematic review and meta-analysis.

Numerous studies have investigated concentrations of lead (Pb) and cadmium (Cd) in rice in China, but have come to divergent conclusions. Therefore we systematically reviewed and meta-analyzed the available evidence on levels of Pb and Cd in rice in different regions of China in order to assess the potential risk to human health. The meta-analysis included 24 studies of Pb levels and 29 studies of Cd levels, published in 2011-2021. The pooled Pb concentration in rice was 0.10 mg per kg dry weight (95% CI 0.08-0.11), while the pooled Cd concentration was 0.16 mg per kg dry weight (95% CI 0.14-0.18). These levels are within the limits specified by national food safety standards. However, the total target hazard quotient for both metals exceeded 1.0 for adults and children, suggesting that rice consumption poses a health risk.

Cultivar-specific response of rhizosphere bacterial community to uptake of cadmium and mineral elements in rice (Oryza sativa L.)

Toxic metal-contaminated farmland from Cadmium (Cd) can enhance the accumulation of Cd and impair the absorption of mineral elements in brown rice. Although several studies have been conducted on Cd exposure on rice, little has been reported on the relationship between Cd and mineral elements in brown rice and the regulatory mechanism of rhizosphere microorganisms during element uptake. Thus, a field study was undertaken to screen japonica rice cultivars with low Cd and high mineral elements levels, analyze the quantitative relationship between Cd and seven mineral elements, and investigate the cultivar-specific response of rice rhizosphere bacterial communities to differences in Cd and mineral uptake in japonica rice. Results showed that Huaidao-9 and Xudao-7 had low Cd absorption and high amounts of mineral nutrient elements (Fe, Zn, Mg, and Ca, LCHM group), whereas Zhongdao-1 and Xinkedao-31 showed opposite accumulation characteristics (HCLM group). Stepwise regression analysis showed that zinc, iron, and potassium are the key minerals that affect Cd accumulation in japonica rice and zinc was the most important factor, accounting for 68.99 %. The accumulation of Cd and mineral elements is potentially associated with rhizosphere soil bacteria. Taxa enriched in the LCHM rhizosphere (phyla Acidobacteriota and MBNT15) indicated the high nutrient characteristics of the soil and reduced activity of Cd in soil. The HCLM rhizosphere was highly colonized by metal-activating bacteria (Actinobacteria), lignin-degrading bacteria (Actinobacteria and Chlorofexi), and bacteria scavenging nutrients and trace elements (Anaerolinea and Ketobacter). Moreover, the differences in the uptake of Cd and mineral elements affected predicted functions of microbial communities, including sulfur oxidation and sulfur derivative formation, human or plant pathogen, and functions related to the iron oxidation and nitrate reduction. The results indicate a potential association of Cd and mineral elements uptake and accumulation with rhizosphere bacteria in rice, thus providing theoretical basis and a new perspective on the maintenance of rice security and high quality simultaneously.

Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice

Melatonin (MT) is participated in plants’ response to cadmium (Cd) tolerance, although its work model remains elusive. Here, the function of MT in adjusting Cd accumulation in rice was investigated. ‘Nipponbare’ (Nip) was cultured in the -Cd (1/2 Kimura B), -Cd + MT (1/2 Kimura B with 1 μM MT), +Cd (1/2 Kimura B plus 1 μM Cd) and +Cd + MT (1/2 Kimura B with 1 μM Cd and 1 μM MT) nutrient solutions for 7 d. Cd markedly induced the endogenous MT accumulation in rice roots and shoots, even within 1 h. MT applied exogenously elevated the hemicelluloses level, which in turn increased the cell wall’s binding capacity to Cd. Furthermore, MT applied exogenously down-regulated the transcription level of Natural Resistance-Associated Macrophage Protein 1 (OsNRAMP1), OsNRAMP5, a major facilitator superfamily gene (OsCd1), and IRON-REGULATED TRANSPORTER 1 (OsIRT1), all of which were responsible for Cd intake, thus less Cd was entered into roots. Moreover, MT applied exogenously also up-regulated transcription level of Cadmium accumulation in Leaf 1 (OsCAL1) and Heavy Metal ATPase 3 (OsHMA3), two genes both attributed to the decreased Cd accumulation in shoots through expelling Cd out of cells and chelating Cd in the vacuoles, respectively. In addition, MT applied exogenously further aggravated the production of nitric oxide (NO) that induced by Cd, while application of a NO donor-SNP mimicked this alleviatory effect of the MT, indicating MT decreased rice Cd accumulation relied on the accumulation of NO.