A simple, fast, and efficient method was developed for preparing radioiodinated 125I-pindolol (125IPIN) using different oxidizing agents. 125I-PIN was prepared by the reaction between Na125I and pindolol using iodogen, Chloramine-T, N-bromosuccinamide, or H2O2 as an oxidizing agent at pH 7. The reaction proceeds well within 30 min at room temperature (20–25°C). The radiochemical yield was determined by TLC using chloroform-acetic acid-water (15: 4: 1) as developing solvent and by HPLC using reversed-phase RP18 column and acetonitrile-0.1 M ammonium bicarbonate (pH 7.5) (1: 1) as the mobile phase at a flow rate of 1 mL min−1. The radiochemical yield with the above oxidants was found to be 85, 93, 96.5, and 97.5%, respectively, with the radiochemical purity of 125I-PIN over 99.9%.