Community Response Research Articles

Empowerment Disaster-Resilient Village Forums for Health Crisis Preparedness: A Participatory Action Research Approach

This study aims to enhance the Disaster Resilient Village (Destana) forum's preparedness for disaster-related health crises. The key challenges identified are low community response to early warning systems and slow mobilization of health resources, both contributing to high victim numbers and exacerbating health crises. The study focuses on creating an accessible early warning system and accelerating health resource mobilization, prioritizing these elements based on an analysis of disaster preparedness parameters. Using Action Research with a Participatory Action Research (PAR) design, the research was conducted in Magetan Regency, East Java, involving 150 Destana forum administrators. The study explores the development of early warning procedures, health contingency plans, emergency response structures, and simulation plans. The findings reveal that the Destana forum successfully developed tailored health contingency plans for each village, including an inclusive early warning system Standard Operating Procedure (SOP) for vulnerable groups. Additionally, an integrated health command structure was formed, enhancing emergency response coordination. Emergency response simulations with 90 participants demonstrated improved readiness and community engagement in handling health crises. The study highlights the importance of a participatory, sustainable approach to building resilience, showing that active community involvement fosters collective responsibility for managing health crises during disasters.

Understorey-nesting bird community responses to land use in the Korup National Park, an Afrotropical rainforest in South-Western Cameroon

Tropical forest biodiversity is under immense pressure due to a combination of global and local factors, including climate change and intensive land use. Birds play an important role in tropical forest ecosystems, contributing significantly to various ecosystem services. This study examined the impact of deforestation on the richness and abundance of understorey-nesting bird communities along a land-use intensification gradient in the peripheral zone of Korup National Park, Southwest Cameroon. We selected 30 sampling plots, each covering 4 ha, across five habitat types: near-primary forest, secondary forest, disturbed forest, cocoa/coffee plantations, and annual food crop fields. A total of 236 understorey bird nests were identified, representing 16 species. Our findings revealed that land use intensity significantly influenced bird species richness and abundance. Primary and secondary forests provided 75% of the nesting species and 71% of the nests. However, 19% of the species either appeared or increased in cocoa/coffee plantations and annual crop fields. Among the feeding groups, frugivores were the most sensitive group to deforestation. This study corroborates the adverse effects of forest destruction on birds communities and highlights the urgent need to address the rapid and extensive forest conversion occurring within Korup National Park. Allowing secondary forests to regenerate and preserving large patches of primary forest are essential strategies to help understorey-nesting bird communities mitigate the impacts of global changes.

Different wetting states in riparian sediment ecosystems: Response to microplastics exposure

Climate change alters the wetting state of riparian sediments, impacting microbial community response and biogeochemical processes. Microplastics (MPs) invade nearly all ecosystems on earth, posing a significant environmental risk. However, little is known about the response mechanism of MP exposure in sediment ecosystems with different wetting states under alternating seasonal rain and drought conditions. In this study, sediments with three different wetting states were selected to explore the differential response of ecosystems to PLA MP exposure. We observed that PLA MP exposure directly affected biogeochemical processes in sediment ecosystems and induced significant changes in microbial communities. PLA MP exposure was found to alter the composition of key species and microbial functional groups in the ecosystem, resulting in a more complex, interconnected, but less stable microbial network. Our findings showed that PLA MP exposure enhances the contribution of stochastic processes, for example the dispersal limitation increasing from 7.41 % to 54.32 %, indicating that sediment ecosystems strive to buffer disturbances from PLA MP exposure. In addition, 87 pathogenic species were detected in our samples, with PLA MPs acting as vectors for their transmission, potentially amplifying ecosystem disturbance. Importantly, we revealed that submerged sediments may present a greater environmental risk, while alternating wet and dry sediments demonstrate greater resistance and resilience to PLA MPs pollution. Overall, this study sheds light on how sediment ecosystems respond to MP exposure, and highlights differences in sediment response mechanisms across wetting states.

Effects of deforestation on multitaxa community similarity in the Brazilian Atlantic Forest.

Habitat loss can lead to biotic homogenization (decrease in β diversity) or differentiation (increase in β diversity) of biological communities. However, it is unclear which of these ecological processes predominates in human-modified landscapes. We used data on vertebrates, invertebrates, and plants to quantify β diversity based on species occurrence and abundance among communities in 1367 landscapes with varying amounts of habitat (<30%, 30-60%, or >60% of forest cover) throughout the Brazilian Atlantic Forest. Decreases in habitat amount below 30% led to increased compositional similarity of vertebrate and invertebrate communities, which may indicate a process of biotic homogenization throughout the Brazilian Atlantic Forest. No pattern was detected in plant communities. We found that habitat loss was associated with a deterministic increase in faunal community similarity, which is consistent with a selected subset of species being capable of thriving in human-modified landscapes. The lack of pattern found in plants was consistent with known variation between taxa in community responses to habitat amount. Brazilian legislation requiring the preservation of 20% of Atlantic Forest native vegetation may be insufficient to prevent the biotic homogenization of faunal communities. Our results highlight the importance of preserving large amounts of habitat, providing source areas for the recolonization of deforested landscapes, and avoiding large-scale impacts of homogenization of the Brazilian Atlantic Forest.

Differential impacts of temperature increase on prokaryotes across temperature regimes in subtropical coastal waters: insights from field experiments

AbstractProkaryotic communities play a dominant role in driving biogeochemical cycling in marine ecosystems. How short‐term temperature increase impacts prokaryotes in subtropical coastal waters is still largely unknown. Here, 14 field experiments were conducted to investigate the response of prokaryotes in subtropical coastal waters to temperature increases of 3°C and 6°C, encompassing a range of ambient temperatures from 17°C to 31°C. We found that responses of prokaryotic growth, grazing pressure, community, and transcriptomes to increased temperatures were largely affected by ambient temperatures. Increased temperatures enhanced the growth rate and grazing pressure of heterotrophic prokaryotes when ambient temperatures were below 26–28°C. The increased temperatures had greater negative effects on the grazing rate compared to the growth rate; therefore, the abundance of heterotrophic prokaryotes generally increased after temperature increase across all temperature regimes. Metatranscriptomics analysis showed that at an ambient temperature of 30°C, genes involved in the adenosine triphosphate synthase were significantly downregulated by the increased temperature. This could be a major factor contributing to the decreased prokaryotic growth rate. In comparison, autotrophic prokaryotes (Synechococcus) exhibited better performance in response to elevated temperatures, thriving up to 35°C, beyond which their growth rate experienced a dramatic decline. When exposing to extremely high temperatures, genes involved in photosynthesis significantly decreased. These findings highlight the differential ecological impacts of temperature increase on prokaryotic communities, varying across different ambient temperatures and taxa in subtropical coastal waters.

Effects of experimental nutrient enrichment and eutrophication on microbial community structure and function in “marine lakes”

Biogeochemical cycling of nitrogen (N) and carbon (C) are regulated by microbial communities that can respond to altered N and C availability, yet the form, consistency, and magnitude of these responses remain poorly constrained. We enriched and eutrophied distinct microbial communities in “marine lakes” through experimental additions of N (5 μM NH4Cl), C (100 μM sucrose), and combined N+C (same forms and concentrations), examining microbial community responses via 16S rRNA sequencing in conjunction with functional responses represented by net community production (NCP) and community respiration (CR) measurements. Individual N or C additions drove significant shifts in microbial community structure only sporadically, and rarely with a corresponding difference in NCP or CR rates. In contrast, the combined addition of N+C elicited strong coincident responses in microbial community structure and function: NCP and CR rates shifted sharply toward heterotrophy and were correlated with multiple microbial networks (r2 = 0.309–0.599, P &amp;lt; 0.001) that included globally distributed marine bacteria. Across multiple experiments, the consistent response of one network, comprised primarily of gammaproteobacterial heterotrophs (particularly Vibrio and Alteromonas), led initially dissimilar communities to converge toward similar composition. However, the distinct response patterns of other more diverse networks were superimposed on top of this network, indicating that inorganic N and organic C enrichment have multilayered effects on microbial communities. Collectively our results demonstrate that elevated N and C alter microbial community structure and function, selecting for multiple microbial networks that compete for, and rapidly cycle, N and C.

Interspecific competition among anemonefishes: pink anemonefish engage in hostile takeovers of sea anemones occupied by clown anemonefish

AbstractUnderstanding drivers of species co-existence on coral reefs is important to predict community responses to environmental change. Here, we present, for the first time, observations of takeovers of anemones occupied by one species, Amphiprion percula, by another species, A. perideraion, and ask if habitat characteristics, fish characteristics, or environmental conditions predict these occurrences. Comparing survey data from multiple years, we find that takeovers occur at a frequency of 0.48–6.84 per 100 groups/year and that this does not depend on disturbances (anemone bleaching) in the population. Takeovers are not predicted by depth, anemone size, A. percula rank 1 size or the number of A. percula in the original group. Instead, we hypothesize that proximity of the A. percula group to the A. perideraion group is a key predictor. Our study demonstrates how long-term observations of tagged individuals and groups could reveal the drivers and consequences of interspecific conflicts on coral reefs.

Host genotype and infection status interact to shape microbiomes in Daphnia magna.

Host–bacterial communities (microbiomes) are influenced by a wide range of factors including host genotype and parasite exposure. However, few studies disentangle temporal and host-genotype-specific variation in microbiome response to infection across several host tissues. We experimentally exposed the freshwater crustacean Daphnia magna to its fungal parasite Metschnikowia bicuspidata and characterized changes in host–bacterial communities associated with the parasite's development within the host. We used 16S rRNA gene sequencing to assess bacterial communities of the host (a) 24 h (‘initial parasite exposure’) and (b) 10 days (‘successful infection’) after exposure to a standard dose of M. bicuspidata spores, in host guts, body tissue (excluding guts) and whole individuals. We also investigated whether bacterial community responses to parasite exposure varied by host genotype.Parasite exposure did not immediately alter host gut bacterial communities, but drove host-genotype-specific changes in the bacterial community composition of whole individuals. We validated that these changes were not driven by shifts in bacterial communities of the culturing medium, due to the addition of the parasite spore solution. Successful infection (i.e. the proliferation of M. bicuspidata spores in the host body) reduced alpha diversity and shifted abundance of dominant bacterial orders in the gut. Moreover, it induced a host-genotype-specific changes in body bacterial community composition. Overall, bacterial community responses to parasite exposure and subsequent infection are complex: they occur in a host-genotype-dependent manner, differentially at distinct timepoints after parasite exposure, and in specific host tissue.

Mapping Community Insights: An Exploratory Analysis on Traffic Management of Davao City, Philippines

Traffic congestion is a growing concern worldwide, including in Davao City, Philippines. Despite efforts to address this issue through technical and engineering-centered strategies, the community-based knowledge is overlooked. This study aims to recognize and ascertain the factors influential to the traffic management in Davao City carved from the quantified community responses through Exploratory Factor Analysis (EFA). Results showed four significant factors namely: 1) Strict Enforcement of Traffic Laws and Strategic Improvements to Infrastructure where high loading factors emphasize the community's strong association between government actions and effective traffic management, underlining that the community acknowledges the local government’s effort to manage the traffic in Davao City (0.775), and the effectivity of traffic lights (0.711) and road improvements (0.613); 2) Traffic Congestion Influences on Community’s Daily Routine where traffic congestion notably impacts daily life, stressing a communal agreement that congestion increases their travel time to work/school (0.824), congestion as a major issue (0.804), and congestion affecting their daily routine (0.767); 3) Community Engagement and Inclusive Approaches where community engagement is deemed crucial for effective traffic solutions, showing the community’s point of view being essential for effective traffic solution (0.715), moderate trust on the local government’s responsiveness (0.657), and desire for the involvement of the non-governmental organizations (NGOs); and 4) Efficient Public Transportation and Infrastructure where public transportation is identified as essential for congestion reduction, accentuating the community’s perspective on the effectiveness of public transportation in reducing traffic congestion (0.583), supporting policies encouraging public transit (0.558), and preference of taking for public transportation instead of driving (0.458).

“Thoroughly Impregnated with Bolshevik Philosophy”

Annie Buller makes for an interesting case study of Canada’s World War II security state and how it functioned vis-à-vis the Communist Party of Canada and its allies. Her experiences speak to gender, party history, and broader elements of political policing, community responses, and confinement experiences. Like her life, her wartime encounters with the Canadian security state were concurrently like and different from those of other criminalized female and male activists at the time about whom we know more. Among the apprehended and incarcerated, female or male, Buller was somewhat of an anomaly and warrants special attention. Buller’s particular situation helps to shed light on lesser-understood elements of the Communist wartime carceral experience, including the lack of trust officials had in these processes at times to accomplish the intended repression and important details about efforts to free those incarcerated. Ultimately, Buller’s case and the movement to liberate her and the other incarcerated members of the party illustrate the power of grassroots activism in challenging oppressive systems.

Effect of substrate concentration on sulfamethoxazole wastewater treatment by osmotic microbial fuel cell: Insight into operational efficiency, dynamic changes of membrane fouling and microbial response

To solve the problems of antibiotic pollution, water resources and energy shortage, an osmotic microbial fuel cell (OsMFC) was adopted innovatively to treat antibiotic wastewater containing sulfamethoxazole (SMX), and achieved SMX removal, water production and electricity generation. Substrate concentration was one of the key factors affecting the performances of OsMFC, but there were few relevant studies This study explored the effect of substrate concentration on system performances, clarified the dynamic changes of membrane fouling under different substrate concentrations, and further revealed the response of microbial communities. The results showed that the stable removal efficiency of SMX exceeded 98.8 % due to the efficient interception of forward osmosis (FO) membrane. Compared with the 1.0 g/L NaAc system, the SMX degradation efficiency and maximum output voltage in the 2.0 g/L NaAc system were only increased by 3.9 % and 6.3 %, respectively. However, the initial water flux decreased by 30.1 % in the 7th cycle due to more serious FO membrane fouling. In addition, there were significant differences in the dynamic formation process of FO membrane fouling. Higher substrate concentration increased the relative abundance of Desulfobacterota and Geobacter. Functional prediction analysis showed that increasing substrate concentration promoted carbohydrate metabolism pathways and relative abundance of sulfur respiration functional groups, thereby improving COD and SMX removal rates. However, the biosynthesis of other secondary metabolites was significantly improved, resulting in increased contents of EPS and SMP, which aggravated membrane fouling. Overall, the system performed better when the substrate concentration was 1.0 g/L. This study would provide certain guidance for the performance optimization and membrane fouling mitigation of OsMFC, thereby promoting its practical application in antibiotic wastewater treatment.

The Functional Response of Estuarine Fish Communities to Hydrologic Change in a Semi-Arid Ecosystem

Functional assessment approaches were used to identify the responses of fish to environmental change in the San Antonio Bay System (Texas, USA). Using a 26-year coastal fisheries dataset (1993–2018), multivariate analyses revealed relationships between functional group abundance and freshwater inflows in the upper segments (Hynes Bay and Guadalupe Bay), but the patterns were decoupled from inflows in the lower bay segments (San Antonio Bay, Ayres Bay and Espiritu Santo Bay). In Hynes and Guadalupe Bays, freshwater migrant carnivores accounted for a significant fraction of the community irrespective of the gear, year or flow. Freshwater stragglers (omnivores and carnivores) were often present in the upper reaches of the bay. In the lower reaches, marine migrant omnivores were present during high and low flows in Espiritu Santos Bay, but only during low flows in Ayres Bay. Marine migrant carnivores were more important in gill nets irrespective of the flow conditions. The five most abundant fish were estuarine resident carnivores and omnivores, accounting for 53.5% of the community. Declines in the abundance of functional groups occurred during the 2011–2014 drought, with rebounds in 2015–2018. Functional methodologies provide insights into estuarine ecosystems and can serve as management tools to assess changes in fish assemblages.

Diel metatranscriptomes capture cyanobacteria-dominated Lake Erie community response to episodic events.

Here, we report on the raw and coassembled metatranscriptomes of 39 Lake Erie surface (1.0 m) water samples collected over a 2-day diel period encompassing episodic weather and bloom events. Preliminary taxonomic annotations and read mappings revealed that Microcystis spp. accounted for up to ~47% of the transcriptionally active community.

Perceptions of a South Australian online trans health resource: transnormativity, safety, and professionalism

Background The proliferation of trans-led online health resources constitutes a purposive community response to the limitations that many trans people face globally in accessing healthcare information and services. While such resources are typically community run or administered by individuals, and hence not subject to reporting mandates, it is useful to examine how end-users perceive online health resources for trans people. Aims This paper reports on an evaluation of one trans-led online health resource, focused on South Australia. Methods Interviews were undertaken with 15 trans people who accessed the website Trans Health SA. Participants were invited to view the website alongside the first author, and to share their thoughts about the website design and content. Results Thematic analysis resulted in the development of three themes: (1) transnormativity and the intended viewer, (2) the website as a welcoming community space, and (3) discrepancies between content and design. Conclusions The paper concludes by considering what it means to create inclusive online trans health resources in the context of both ongoing marginalization and community diversity.

Response of Alfalfa Leaf Traits and Rhizosphere Fungal Communities to Compost Application in Saline-Sodic Soil.

Soil salinization is considered a major global environmental problem due to its adverse effects on agricultural sustainability and production. Compost is an environmentally friendly and sustainable measure used for reclaiming saline-sodic soil. However, the responses of the physiological characteristics of alfalfa and the structure and function of rhizosphere fungal communities after compost application in saline-sodic soil remain elusive. Here, a pot experiment was conducted to explore the effect of different compost application rates on soil properties, plant physiological traits, and rhizosphere fungal community characteristics. The results showed that compost significantly increased soil nutrients and corresponding soil enzyme activities, enhanced leaf photosynthesis traits, and ion homeostasis compared with the control treatment. We further found that the rhizosphere fungal communities were dominated by Sodiomyces at the genus level, and the relative abundance of pathogenic fungi, such as Botryotrichum, Plectosphaerella, Pseudogymnoascus, and Fusarium, declined after compost application. Moreover, the α-diversity indexes of the fungal community under compost application rates of 15% and 25% significantly decreased in comparison to the control treatment. The soil SOC, pH, TP, and TN were the main environmental factors affecting fungal community composition. The leaf photosynthetic traits and metal ion contents showed significantly positive correlations with Sodiomyces and Aspergillus. The fungal trophic mode was dominated by Pathotroph-Saprotroph-Symbiotroph and Saprotroph. Overall, our findings provide an important basis for the future application of microbial-based strategies to improve plant tolerance to saline-alkali stress.

Resilience of Phytoplankton and Microzooplankton Communities under Ocean Alkalinity Enhancement in the Oligotrophic Ocean.

Ocean alkalinity enhancement (OAE) is currently discussed as a potential negative emission technology to sequester atmospheric carbon dioxide in seawater. Yet, its potential risks or cobenefits for marine ecosystems are still mostly unknown, thus hampering its evaluation for large-scale application. Here, we assessed the impacts OAE may have on plankton communities, focusing on phytoplankton and microzooplankton. In a mesocosm study in the oligotrophic subtropical North Atlantic, we investigated the response of a natural plankton community to CO2-equilibrated OAE across a gradient from ambient alkalinity (2400 μmol kg-1) to double (4800 μmol kg-1). Abundance and biomass of phytoplankton and microzooplankton were insensitive to OAE across all size classes (pico, nano and micro), nutritional modes (autotrophic, mixotrophic and heterotrophic) and taxonomic groups (cyanobacteria, diatoms, haptophytes, dinoflagellates, and ciliates). Consequently, plankton communities under OAE maintained their natural chlorophyll a levels, size structure, taxonomic composition and biodiversity. These findings suggest a high tolerance of phytoplankton and microzooplankton to CO2-equilibrated OAE in the oligotrophic ocean. However, alternative application schemes involving more drastic perturbations in water chemistry and nutrient-rich ecosystems require further investigation. Nevertheless, our study on idealized OAE will help develop an environmentally safe operating space for this climate change mitigation solution.

Characterization of T and B cell epitopes in PvCyRPA by studying the naturally acquired immune response in Brazilian Amazon communities

Plasmodium vivax, a challenging species to eliminate, causes millions of malaria cases globally annually. Developing an effective vaccine is crucial in the fight against vivax malaria, but considering the limited number of studies focusing on the identification and development of P. vivax-specific vaccine candidates, exploring new antigens is an urgent need. The merozoite protein CyRPA is essential for P. falciparum growth and erythrocyte invasion and corresponds to a promising candidate antigen. In P. vivax, a single study with multiple vaccine candidates indicates PvCyRPA with strong association with protection, outperforming classic malaria vaccine candidates. However, little is known about the specific naturally acquired response in the Americas, as well as the antigen epitope mapping. For this reason, we aimed to investigate the cellular and humoral immune response elicited against PvCyRPA in Brazilian endemic areas to identify the existence of immunodominant regions and the potential of this protein as a single or even a multi-stage specific malaria vaccine candidate for P. vivax. The results demonstrated that PvCyRPA is naturally immunogenic in Brazilian Amazon individuals previously exposed to malaria, which presented anti-PvCyRPA cytophilic antibodies. Moreover, our data show that the protein also possesses important immunogenic regions with an overlap of B and T cell epitopes. These data reinforce the possibility of including PvCyRPA in vaccine formulations for P. vivax.

Understanding seismic hazard resilience in Montenegro: A qualitative analysis of community preparedness and response capabilities

Abstract Enhancing resilience against seismic hazards in earthquake-prone regions is essential for reducing the devastating impacts of disasters. Seismic resilience refers to a community’s ability to withstand and recover from earthquake impacts, while preparedness gaps are the areas where current measures are insufficient to effectively respond to or mitigate earthquake damage. This study focuses on Montenegro – a region with frequent seismic activity – aiming to assess resilience levels, identify critical gaps in preparedness, and evaluate the effectiveness of existing response strategies. Using qualitative methods, including semi-structured interviews, the research gathered insights from residents of Montenegro’s most vulnerable cities: Nikšić, Podgorica, Bar, Kotor, Cetinje, Budva, Herceg Novi, and Berane. Participants, chosen for their first-hand experience with significant earthquake impacts, provided valuable perspectives on various aspects of resilience, from local government response to individual preparedness. This research revealed significant disparities in resilience across communities: for instance, approximately 62.5% of the respondents highlighted inadequate education as a barrier to effective earthquake preparedness, and only 37.5% reported awareness of basic earthquake response procedures. Furthermore, while some communities, such as urban areas with accessible services, reported higher preparedness levels, rural areas showed deficiencies, with 50% of the respondents from these areas identifying a lack of organized drills and limited public awareness initiatives. These findings underscore the urgent need for community-specific preparedness programs and enhancements in both structural resilience and public education to bolster community readiness effectively. Also, findings highlight the need for customized preparedness programs tailored to specific community needs, alongside improvements in structural safety measures and educational outreach. The study underscores the importance of a comprehensive approach involving detailed risk assessments, community-focused preparedness training, and stronger public awareness initiatives. Furthermore, the study calls for enhanced local government capabilities to sustain proactive response measures, including rapid mobilization of emergency resources and regular disaster simulations, to build long-term resilience across communities.

INTEGRATING INTERTEXTUAL AND COMMUNITY RESPONSE ANALYTICAL LENSES TO TURN SYAIR CENDAWAN PUTIH’S A CHILDREN'S STORY “THE ADVENTURE OF CENDAWAN PUTIH” INTO A SCIENCE LEARNING

This study explores the transformation of the Cendawan Putih poem into prose suitable for children's science learning, examining the adaptation process and public perception. Using a qualitative approach, the study employs intertextual theory to analyze textual transformations such as expansion, conversion, modification, and citation and community response theory to capture stakeholders’ views. The research investigates how the Cendawan Putih poem has been creatively adapted into the children’s story The Adventure of Cendawan Putih to enhance science learning while maintaining cultural and educational value. The findings reveal that adapting Cendawan Putih for children’s literature not only preserves core narrative elements but also integrates science concepts relevant to young readers. The adaptation utilizes techniques such as expansion and modification, enriching the original storyline to highlight nature, ecosystems, and environmental science in an accessible format for children. Public feedback, collected through Focus Group Discussions (FGDs) with cultural figures, educators, academics, and youth, shows broad support for this transformation. Stakeholders emphasize the story’s potential to foster cultural awareness and introduce foundational science concepts, making it an innovative tool for learning. This study highlights how traditional literary forms can be reimagined for children’s science learning, merging cultural heritage with modern educational needs. By presenting Cendawan Putih in a format that supports both cultural preservation and science education, this work positions the story as an intergenerational bridge and a valuable addition to literature-based science learning. It underscores the importance of community involvement in literary adaptation, providing new insights for literature, cultural education, and science pedagogy.

Strengthening Community Responses to Economic vulnerability (SeCuRE): a protocol of an HIV status-neutral pilot randomized clinical trial with transgender women of color in Detroit, Michigan

BackgroundIn the United States (US), transgender women of color experience cyclical, interlocking systems of structural and institutional oppression rooted in racism and transphobia, which fuel economic vulnerability. Together, cycles of intersecting racism, transphobia, and economic vulnerability create conditions that give rise to extreme HIV inequities among transgender women of color. Microeconomic interventions — designed to improve financial standing by increasing income generation and access to financial resources through entrepreneurship, cash transfers, and training — have the potential to address structural factors underlying HIV inequities. Over the past few years, several trans-led organizations, including the Trans Sistas of Color Project, have integrated microeconomic strategies, specifically emergency assistance, into their programming. The aim of the current study is to conduct a pilot randomized controlled trial (RCT) to evaluate the feasibility and acceptability of a definitive subsequent RCT and explore initial evidence of an enhanced microeconomic intervention to increase income generation and improve HIV prevention and care continua outcomes.MethodsThis is a two-arm waitlist randomized controlled trial in which transgender women of color will be randomly allocated to either usual care that includes the Trans Sistas of Color Project’s existing microeconomic interventions, which includes the following: (1) US $250 in emergency assistance and (2) peer support to obtain legal gender affirmation (i.e., legal name and gender markers on identification documents) or the enhanced microeconomic intervention that includes usual care and will be enhanced to include the following: (1) 12 weekly educational group sessions on economic empowerment (i.e., job acquisition, income generation through micro-business, and financial literacy) and HIV prevention and care, (2) employment-focused mentoring, and (3) an unconditional grant (US $1200) for use towards acquiring self-led or formal employment. Participants in each condition will complete a baseline survey prior to randomization, a follow-up survey immediately following intervention completion, and 3-month survey after intervention completion. Participants will also complete qualitative exit interviews within 1 month of intervention completion for both conditions.DiscussionThis study will be one of the first US-based pilot randomized clinical trials that builds upon existing community-led solutions to economic vulnerability to address HIV inequities. Findings will provide the necessary groundwork to examine intervention effectiveness in a future large-scale trial.Trials registrationNCT06212544.Protocol versionSeptember 25, 2024, version 2.