This one-year study evaluated the effects of integrated chemical (phosphate and phosphite) and biological (arbuscular mycorrhizal fungi; AMF) biostimulants on the postharvest performance of cut Rosa hybrida cv. Dolce Vita grown in soilless culture. The experiment was conducted as a factorial arrangement in a completely randomized design (CRD) with 29 phosphorus treatments applied via nutrient solution and foliar spray under both AMF-inoculated and non-inoculated conditions, with four replications per treatment. Preliminary optimization using response surface methodology (RSM) identified 17 treatments with the highest overall desirability for enhancing soluble sugars, total chlorophyll, protein, phenolics, and flavonoids. Postharvest analyses revealed that combined potassium-phosphite and phosphate treatments with AMF significantly improved relative solution uptake (RSU, 18-29%; P < 0.01), relative fresh weight (RFW, 21-28%; P < 0.01), and chlorophyll index (CI, 22-27%; P < 0.01), while reducing ion leakage, malondialdehyde (MDA), and hydrogen peroxide (H₂O₂) accumulation compared to the control. Biochemical assays showed increases in soluble protein (up to 1.84mg g⁻¹ FW), soluble sugars (up to 2.92mg g⁻¹ FW), total phenolics (up to 2.99mg gallic acid g⁻¹ FW), flavonoids (up to 0.384mg quercetin g⁻¹ FW), and enhanced activities of phenylalanine ammonia-lyase (PAL) and antioxidant enzymes (CAT, APX, GPX, SOD; P < 0.01). Gene expression analyses confirmed upregulation of PAL and SOD, supporting enzymatic and molecular defense activation. These integrated treatments effectively delayed senescence, reduced neck bending, and extended vase life from 21 days in the control to 29 days in the optimal treatment (25% potassium-phosphite in nutrient solution combined with 50:50 foliar phosphate/phosphite plus AMF). Overall, the combined application of phosphite, phosphate, and AMF enhances physiological, biochemical, and molecular resilience in cut roses, providing a practical approach for improving postharvest quality.

Sonodynamic therapy (SDT), as a promising noninvasive therapeutic modality with superior penetration depth, receives tremendous attention. To date, the widely accepted mechanism for reactive oxygen species (ROS) generation in SDT involves acoustic cavitation-triggered sonoluminescence (SL), followed by the SL-activation of sonosensitizers. However, current research on sonosensitizer development primarily focuses on promoting SL-to-ROS conversion, overlooking the essential role of the cavitation process. To fully unleash the potential of SDT, herein, a dual-enhanced strategy that harnesses the enhanced cavitation for SL generation and efficient SL-to-ROS conversion is developed for the first time to realize an all-around enhancement of SDT. Specifically, the proposed nano-sonosensitizer, namely MeTTh-PAE NPs, is released as hydrophobic aggregates with a rough surface in response to an acidic environment, allowing for highly enhanced cavitation-triggered SL under ultrasound. Meanwhile, as a typical aggregation-induced emission molecule, MeTTh demonstrates a highly promoted intersystem crossing process at its aggregated state, facilitating efficient SL-to-ROS conversion. Notably, combining these two fascinating attributes in MeTTh-PAE NPs results in an excellent sonodynamic antitumor effect in both in vitro and in vivo. This work proposes a novel strategy to fully exploit the potential of SDT and provides valuable insights for advancing the design of nano-sonosensitizers.

Removal of uranium from aqueous solution using cellulose extracted from the leaves of Musa paradisiaca.

Re-investigation of the relevant factors affecting quantitative precipitation of wine polysaccharides: A GC-MS and HRSEC-RI characterization study using central composite design and response surface methodology.

Machine learning coupling microbial succession to decipher gas stripping-reinforced sulfate reduction for hydrogen sulfide removal.

Mix design optimization via response surface methodology and flexural performance of beams in solid alkali-activated fly ash-based concrete

Optimized biodiesel production from palm kernel and Jatropha curcas oil blend using KOH-supported calcined animal bone catalyst: A response surface methodology and genetic algorithm-Bayesian hybridization

Multi-objective optimization of recycled fine aggregate geopolymer-based grouting materials using the response surface methodology and backpropagation neural network coupled approach

Optimizing 2-(4-(3-ethylpentan-3-yl) phenoxy) acetic acid for lithium recovery from aqueous solution through response surface methodology

Biofuel production from food waste and rice straw via microwave co-pyrolysis: Product characterization and optimization using Box-Behnken design and response surface methodology

Optimizing interlayer adhesion of FFF-printed PEEK on an accessible platform via short-beam shear testing and response surface methodology

Optimization of carbon capture and storage technology using sodium alginate through response surface methodology

A stable reversed-phase high-performance liquid chromatography method was developed and validated to quantify related substances in nitrofurantoin capsule formulations. Based on response surface methodology and experimental design, the Box-Behnken design technique improved method performance and dependability. The mobile phase-A was formulated by mixing 0.2% formic acid solution and tetrahydrofuran in the ratio of 95:5% (v/v), and the mobile phase-B was formulated by mixing methanol, acetonitrile, and tetrahydrofuran in the ratio of 50:40:10 (v/v/v) using gradient elution with a flow rate of 0.8 mL/min. Chromatographic separation was achieved using an Inertsil ODS-3 C18 column (250 mm × 4.6 mm, 5 μm), with detection at 285 nm and column temperature maintained at 30°C. The method was validated according to ICH and USP <1225> guidelines. This is demonstrated by high-level accuracy, covering an entire range from 92% to 111%. Precision (resistance ≤ 1.0%) and linearity (R2 > 0.999). Box-Behnken design-based RP-HPLC technique for nitrofurantoin formulations is significant because it uses a systematic, statistically optimized approach to method development to assure robustness, accuracy, and dependability. This work provides a proven stability-indicating approach for routine quality control and regulatory compliance to address safety concerns about nitrofurantoin degradation products and impurities.

Optimization of bacterial disinfection in drinking water by ozone-induced oxidation-reduction potential control using response surface methodology

Oily-wastewater treatment using eco-friendly ceramic membranes derived from sugarcane bagasse waste: Optimization using response surface methodology

Enhanced thermal performance and entropy management in a Y-shaped cavity with an inner rectangular Vertical Wall: A computational study with sensitivity analysis using response surface methodology

Kiwi peel waste enhances manure protein degradation: Statistical optimization using Box-Behnken design and response surface methodology

Antioxidant hybrid pigments developed by optimization of ascorbic acid loading into halloysite nanotubes via response surface methodology and co-loading with antioxidant-rich natural colorant: a functional food ingredient

Optimizing biogas production through the co-digestion of tannery fleshing, cowdung, and sewage water using response surface methodology

Optimization of welding technological parameters and mechanical properties of thin plate based on response surface methodology