Respective Threshold Values Research Articles

Bradyrhizobium ontarionense sp. nov., a novel bacterial symbiont isolated from Aeschynomene indica (Indian jointvetch), harbours photosynthesis, nitrogen fixation and nitrous oxide (N2O) reductase genes

A novel bacterial symbiont, strain A19T, was previously isolated from a root-nodule of Aeschynomene indica and assigned to a new lineage in the photosynthetic clade of the genus Bradyrhizobium. Here data are presented for the detailed genomic and taxonomic analyses of novel strain A19T. Emphasis is placed on the analysis of genes of practical or ecological significance (photosynthesis, nitrous oxide reductase and nitrogen fixation genes). Phylogenomic analysis of whole genome sequences as well as 50 single-copy core gene sequences placed A19T in a highly supported lineage distinct from described Bradyrhizobium species with B. oligotrophicum as the closest relative. The digital DNA-DNA hybridization and average nucleotide identity values for A19T in pair-wise comparisons with close relatives were far lower than the respective threshold values of 70% and ~ 96% for definition of species boundaries. The complete genome of A19T consists of a single 8.44 Mbp chromosome and contains a photosynthesis gene cluster, nitrogen-fixation genes and genes encoding a complete denitrifying enzyme system including nitrous oxide reductase implicated in the reduction of N2O, a potent greenhouse gas, to inert dinitrogen. Nodulation and type III secretion system genes, needed for nodulation by most rhizobia, were not detected. Data for multiple phenotypic tests complemented the sequence-based analyses. Strain A19T elicits nitrogen-fixing nodules on stems and roots of A. indica plants but not on soybeans or Macroptilium atropurpureum. Based on the data presented, a new species named Bradyrhizobium ontarionense sp. nov. is proposed with strain A19T (= LMG 32638T = HAMBI 3761T) as the type strain.

Revisiting investment and its determinants through threshold analysis

AbstractSince the time of classical economists, investment decisions hold centre stage in economic theory. In this article, we integrate classical economists' perspectives on the determinants of investment with the Keynesian theory of effective demand. For this purpose, we employ variables to capture the effects of profitability, the state of demand, and the financial and risk conditions using time series data from 17 major OECD economies spanning the 1960–2017 period. Two are the salient features of our article: The first is the use as profitability variables, the marginal efficiency of capital or the incremental rate of return, and the second is the use of regime changes and respective threshold values for these two key variables. The econometric results show that the profitability variables are decisive in shaping investment decisions and designating phase changes.

Effect and potential mechanisms of sludge-derived chromium, nickel, and lead on soil nitrification: Implications for sustainable land utilization of digested sludge

Increasing occurrence of heavy metals (HMs) in sewage sludge threatens its widespread land utilization in China due to its potential impact on nutrient cycling in soil, requiring a better understanding of HM-induced impacts on nitrification. Herein, lab-scale experiments were conducted over 185-day, evaluating the effect of sludge-derived chromium (Cr3+), nickel (Ni2+), and lead (Pb2+) on soil nitrification at different concentrations. Quantitative polymerase chain reaction and linear regression results revealed an inhibitory sequence of gene abundance by HMs’ labile fraction: ammonia-oxidizing bacteria (AOB)-ammonia monooxygenase (amoA)> nitrite oxidoreductase subunit alpha (nxrA)> nitrite oxidoreductase subunit beta (nxrB). The toxicity of HMs’ incremental labile fraction decreased in the order of Ni2+>Cr3+>Pb2+, with respective threshold values of 5.01, 24.03 and 38.42 mg·kg−1. Furthermore, extending incubation time reduced HMs inhibition on ammonia oxidation, mainly related to their fraction bound to carbonate minerals. Random Forest analysis, variation partitioning analysis, and Mantel test indicated that soil physicochemical properties primarily affected nitrification genes, especially in the test of Cr3+ on AOB-amoA, nxrA, nxrB, Ni2+ for complete ammonia-oxidizing bacteria-amoA, and Pb2+ for nxrA and nxrB. These findings underline the importance of labile HMs fractions and soil physicochemical properties to nitrification, guiding the establishment of HM control standards for sludge utilization.

Derivation of threshold values for the sulfur nutritional status of European silver fir from a cumulative concentration distribution

AbstractFor European silver fir (Abies alba (Mill)), reliable threshold values for the sulfur nutritional status are not available. Because European silver fir is an important coniferous tree species in Central Europe and due to the fact that by reduced S emissions, S is becoming a more and more critical nutrient this knowledge gap should be closed. From the interpretation of the cumulative distribution of sulfur concentrations in first‐year needles, the respective threshold values for the range of normal nutrition as well as the threshold for deficiency could be derived. When knowing these thresholds also the range of harmonic nutrition, which is the range for the optimal relation of two nutrients, could be calculated. As compared to the very few literature data available and compared to the respective values of the two most important European conifers Norway spruce (Picea abies) and Scots pine (Pinus sylvestris), the values derived for S nutrition of European silver fir are very reasonable. With respect to the S nutritional thresholds, European silver fir is very close to the values of the other two conifers, whereas the harmonic range of S in relation to N is a bit shifted to higher values indicating that European silver fir has a slightly higher demand for S (in relation to N). There is a certain time lag between the reduction of SO2 emissions in Europe and the reduction of S concentrations in needles, suggesting that S stored in the soil during periods of high emissions is relevant for S nutrition of trees for a longer period of time.

A large strain anisotropic ductile damage model — Effective driving forces and gradient-enhancement of damage vs. plasticity

A novel anisotropic ductile damage model at finite strains is proposed in this work. The model is formulated based on the fictitious configuration concept and includes deformation induced evolution of anisotropic damage properties. As a key contribution, different effective driving forces for both, plasticity and damage are elaborated in the context of a so-called two-surface formulation, i.e. the consideration of two admissible (but not independent) domains — one related to plastic flow and one related to damage evolution. The investigations include the introduction of effective stresses driving plastic flow on the one hand, and effective driving forces related to the evolution of anisotropic damage on the other. In addition and as an alternative to the introduction of effective driving forces, contributions influencing the respective threshold values related to plastic flow and damage evolution are proposed. Specification of the underlying constitutive equations together with the introduction of a suitable algorithmic implementation allows the model to predict experimentally observed material behaviour. Moreover, fundamental properties of the model are discussed by means of non-proportional loading paths generated by combinations of loading under uniaxial tension and simple shear. In view of the simulation of inhomogeneous boundary value problems, the implementation of the model into a finite element formulation is discussed. Since softening, respectively material degradation and necking phenomena, may results in mesh-dependent simulation results, an appropriate regularisation must be included in the model. In this work, gradient-enhancements in the form of a so-called micromorphic approach are included. In particular, gradients of scalar-valued hardening variables related to plasticity and damage are accounted for. A detailed analysis of the necessity of these gradient-enhancements of both, damage and plasticity is carried out based on the proposed multi-surface anisotropic ductile damage model. It turns out that gradient-enhanced regularisation of the damage contribution alone is not sufficient to regularise the problem (and vice versa, i.e. gradient-enhanced regularisation of the plasticity contribution alone is not sufficient as well) but contributions related to both phenomena, i.e. damage and plasticity, need to be regularised.

Ensifer canadensis sp. nov. strain T173T isolated from Melilotus albus (sweet clover) in Canada possesses recombinant plasmid pT173b harbouring symbiosis and type IV secretion system genes apparently acquired from Ensifer medicae.

A bacterial strain, designated T173T, was previously isolated from a root-nodule of a Melilotus albus plant growing in Canada and identified as a novel Ensifer lineage that shared a clade with the non-symbiotic species, Ensifer adhaerens. Strain T173T was also previously found to harbour a symbiosis plasmid and to elicit root-nodules on Medicago and Melilotus species but not fix nitrogen. Here we present data for the genomic and taxonomic description of strain T173T. Phylogenetic analyses including the analysis of whole genome sequences and multiple locus sequence analysis (MLSA) of 53 concatenated ribosome protein subunit (rps) gene sequences confirmed placement of strain T173T in a highly supported lineage distinct from named Ensifer species with E. morelensis Lc04T as the closest relative. The highest digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of genome sequences of strain T173T compared with closest relatives (35.7 and 87.9%, respectively) are well below the respective threshold values of 70% and 95-96% for bacterial species circumscription. The genome of strain T173T has a size of 8,094,229 bp with a DNA G + C content of 61.0 mol%. Six replicons were detected: a chromosome (4,051,102 bp) and five plasmids harbouring plasmid replication and segregation (repABC) genes. These plasmids were also found to possess five apparent conjugation systems based on analysis of TraA (relaxase), TrbE/VirB4 (part of the Type IV secretion system (T4SS)) and TraG/VirD4 (coupling protein). Ribosomal RNA operons encoding 16S, 23S, and 5S rRNAs that are usually restricted to bacterial chromosomes were detected on plasmids pT173d and pT173e (946,878 and 1,913,930 bp, respectively) as well as on the chromosome of strain T173T. Moreover, plasmid pT173b (204,278 bp) was found to harbour T4SS and symbiosis genes, including nodulation (nod, noe, nol) and nitrogen fixation (nif, fix) genes that were apparently acquired from E. medicae by horizontal transfer. Data for morphological, physiological and symbiotic characteristics complement the sequence-based characterization of strain T173T. The data presented support the description of a new species for which the name Ensifer canadensis sp. nov. is proposed with strain T173T (= LMG 32374T = HAMBI 3766T) as the species type strain.

Reputation based novel trust management framework with enhanced availability for cloud

To select a cloud service provider (CSP) out of immense number of relevant CSPs available, cloud customer (CC) trusts on the reviews provided by the CCs who have used the services provided by CSPs. However, relying on these feedbacks may be hazardous if the review(s) have been deliberately posted with wicked motive. To mitigate the impact of malicious feedbacks, a framework, namely highly available credible cloud trust management framework (HA2CTMF) has been proposed with the intent of revamping the reputation and availability parameters of QoS by evaluating the trust of cloud service providers (CSPs) based on the credibility of feedbacks furnished by CC. HA2CTMF detects collusion and Sybil attacks on the labeled CloudArmor dataset and increases availability of the trust service. Collusion attack has been detected by comparing review count; service count; & mean of reviews with their respective threshold values. Further, Sybil attack has been uncovered with the help of these aspects: deviation from mean of reviews; pattern detection; & comparison of polarity value from both lower & upper threshold values. Additionally, enhanced availability has been ensured by working on disaster management and diminishing repercussions of single point of failure. The results have been accessed on the following parameters: precision; recall; f1-score & availability percentage on the dataset comprising 8774 data points. This framework has exhibited precision of 85.69%, recall of 89.45%, f1-score of 87.53% and availability of 96.71% compared to CloudArmor framework values on the same parameters as: 44.47%; 62.21%; 51.85% & 70.43% respectively. As per the results attained, we conclude that proposed HA2CTMF has demonstrated better outcome.

Low-energy electron interaction with 2-(trifluoromethyl)acrylic acid, a potential component for EUVL resist material.

Motivated by the current introduction of extreme ultraviolet lithography (EUVL) into chip manufacturing processes, and thus the transition to electron-induced chemistry within the respective resist materials, we have studied low energy electron-induced fragmentation of 2-(trifluoromethyl)acrylic acid (TFMAA). This compound is chosen as a potential resist component, whereby fluorination enhances the EUV adsorption and may at the same time promote electron-induced dissociation. Dissociative ionization and dissociative electron attachment are studied, and to aid the interpretation of the observed fragmentation channels, the respective threshold values are calculated at the DFT and coupled cluster level of theory. Not surprisingly, we find significantly more extensive fragmentation in DI than in DEA and in fact, the only significant DEA fragmentation channel is the cleavage of HF from the parent molecule upon electron attachment. Rearrangement and new bond formation are substantial in DI and are, in fact, similar to DEA, mainly associated with HF formation. The observed fragmentation reactions are discussed in relation to the underlying reactions and potential implications for the suitability of TFMAA as a component of EUVL resist materials.

An Improved VME Technique via Heap Based Optimization Algorithm and AWIT Method for PLI and MA Noise Elimination in ECG

Diagnosing cardiac conditions require careful examination of an electrocardiogram (ECG). However, a significant issue arises when capturing an ECG due to interference from various noises. Noises like power line interference (PLI) and muscle artifact (MA) change the morphology, making it difficult to interpret the original signal. Our research proposes an improved variational mode extraction (IVME) technique using a Heap-based optimization (HBO) algorithm and an automatic wavelet interval-dependent thresholding (AWIT) method to eliminate such noises. First, HBO uses the envelope entropy spectrum (EES) as the objective function to find the best fitness value for optimizing the VME parameter, known as penalty factor <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\alpha $ </tex-math></inline-formula> . Then, we extract a specific mode using the optimal <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\alpha $ </tex-math></inline-formula> value in VME to accurately remove PLI from the signal. Finally, the AWIT method automatically computes the intervals and their respective threshold values to remove excessive MA noise from the PLI-filtered ECG signal. We evaluate the efficiency of ten random real-time ECG signals from the MIT-BIH arrhythmia database. The result analysis proves that our algorithm can accurately extract the mode containing PLI and eradicate MA from the noisy ECG signal. It also shows improvement in signal parameters like signal-to-noise ratio (SNR <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$_{\mathrm {improvement}}$ </tex-math></inline-formula> ), mean square error (MSE), and correlation coefficients (CC) with 36.7968 dB, 0.00030901, and 99.7278%, respectively.

Distribution and ecological risk assessment of heavy metals in sediments of Dajiuhu Lake Wetland in Shennongjia, China.

The rapid development of modern society has resulted in discharge of large, heavy metal quantities into wetlands that have been continuously accumulating, causing severe pollution. Dajiuhu, located in the Shennongjia Forest District of Hubei Province in China, is a wetland of significant value internationally, serving as a model wetland ecosystem with heightened scientific research value. In this study, 27 surface sediment samples from nine sub-lakes in Dajiuhu were collected in August 2020. The concentrations of Cd, Cr, Cu, Ni, Pb, and Zn in the sediments were determined. The heavy metal occurrence and speciation characteristics were analyzed by an improved BCR (European Community Bureau of Reference) extraction method. Four methods were used to evaluate heavy metals' pollution degree and ecological risk. The possible source of heavy metals was inferred using correlation analysis and principal component analysis. The heavy metal content in the lake sediments of Dajiuhu wetland was from the highest to the lowest concentration as follows: Zn [Formula: see text] Cr [Formula: see text] Ni [Formula: see text] Pb [Formula: see text] Cu [Formula: see text] Cd. The average Cd content exceeded the national nature reserve threshold values, while the other heavy metals measured were below their respective threshold values. However, due to the occurrence of Pb and Cd in different forms, they still pose certain pollution and ecological risk to the lake wetlands. On the other hand, Zn, Cr, Ni, and Cu do not pose an ecological risk in the lakes of the Dajiuhu wetland. The spatial distribution of heavy metal content in the nine sub-lakes did vary significantly. Regarding the heavy metal sources in the lake sediments, Ni, Cr, and Cu originate from natural factors, and Cd and Pb have mainly anthropogenic origins. In contrast, Zn has both natural and anthropogenic origins. This study provides further insights into the study of heavy metal pollution in lake wetlands. It provides a framework and a direction for managing heavy metal pollution in the Dajiuhu wetland.

Decoding the Specific Roasty Aroma Wuyi Rock Tea (Camellia sinensis: Dahongpao) by the Sensomics Approach.

Aroma extract dilution analysis was performed on volatile fractions extracted from a freshly prepared Dahongpao (DHP) tea infusion using solvent-assisted flavor evaporation, yielding 65 odor-active domains with flavor dilution factors ranging between 32 and 32,768. In addition, six aromatic substances were captured by headspace analysis. Quantitation of 54 compounds by an internal standard method and stable isotope dilution assays revealed that the concentrations of 32 odorants exceeded their respective orthonasal odor threshold values in tea infusion. The results of odor activity values (OAVs) suggested that 2-metylbutanal (malty) and γ-hexalactone (coconut-like) had the highest OAVs (248 and 154). Eight odorants including γ-hexalactone (OAV 154), methyl 2-methylbutanoate (59), phenylacetic acid (7.2), acetylpyrazine (5.7), 2-methoxyphenol (3.4), p-cresol (2.7), 2,6-diethylpyrazine (2.7), and vanillin (1.8) were newly identified as key odorants in DHP tea infusion. An aroma recombination model in a non-volatile matrix extracted from tea infusion satisfactorily mimicked the overall aroma of DHP tea infusion, thereby confirming the identification and quantitative experiments. Omission experiments verified the obvious significance of 6-methyl-5-hepten-2-one (OAV 91), 2-ethyl-3,5-dimethylpyrazine (19), 4-hydroxy-2,5-dimethylfuran-3(2H)-one (13), and acetylpyrazine (5.7) as key odorants for the special roasty and caramel-like aroma of DHP tea.

Comparison of In Vitro and In Planta Heavy Metal Tolerance and Accumulation Potential of Different Armeria maritima Accessions from a Dry Coastal Meadow.

The aim of the present study was to compare the tolerance to several heavy metals and their accumulation potential of Armeria maritima subsp. elongata accessions from relatively dry sandy soil habitats in the Baltic Sea region using both in vitro cultivated shoot explants and long-term soil-cultivated plants at the flowering stage as model systems. The hypothesis that was tested was that all accessions will show a relatively high heavy metal tolerance and a reasonable metal accumulation potential, but possibly to varying degrees. Under the conditions of the tissue culture, the explants accumulated extremely high concentration of Cd and Cu, leading to growth inhibition and eventual necrosis, but the accumulation of Pb in their tissues was limited. When grown in soil, the plants from different accessions showed a very high heavy metal tolerance, as the total biomass was not negatively affected by any of the treatments. The accumulation potential for heavy metals in soil-grown plants was high, with several significant accession- and metal-related differences. In general, the heavy metal accumulation potential in roots and older leaves was similar, except for Mn, which accumulated more in older leaves. The absolute higher values of the heavy metal concentrations reached in the leaves of soil-grown A. maritima plants (500 mg Cd kg−1, 600 mg Cu kg−1, 12,000 mg Mn kg−1, 1500 mg Pb kg−1, and 15,000 mg Zn kg−1) exceeded the respective threshold values for hyperaccumulation. In conclusion, A. maritima can be characterized by a species-wide heavy metal tolerance and accumulation potential, but with a relatively high intraspecies diversity.

Superlubricity and running-in wear maps of water-lubricated dissimilar ceramics

Water-lubricated ceramic sliding tribosystems, when operating under superlubricity conditions, reduce frictional energy losses in many applications. Aqueous superlubricity of 5 dissimilar ceramic tribopairs (Si3N4–Al2O3, Si3N4-ZTA, Si3N4–SiC, Al2O3–SiC, ZrO2–SiC) was thus studied in a series of ball-on-disk tests, with the identification of wear mechanisms and lubrication regimes, while introducing a running-in wear map approach to elucidate transient tribological phenomena. All pairs presented superlubricity for certain combinations of normal load and sliding speed, after a running-in period where mechanical and thermal severities of contact remained mostly below their respective threshold values, promoting mild wear regimes. Load-carrying capacity of ZrO2–SiC was the highest due to high homogeneity and low roughness of the tribofilm on SiC disk’s wear track.

ANALYZING PRICE TRANSMISSION IN THE NIGERIAN CATTLE MARKET

The study analysed price transmission in the Nigerian cattle market. Time series price data, for ten states (Borno, Cross River, Edo, Kano, Nassarawa, Osun, Oyo, Plateau, Yobe and Zamfara) for the years, 2002-2017 were used for the analysis. The summarised result on Threshold Vector Error Correction model showed that positive and negative deviations for eight states (Borno, Cross River, Edo, Nassarawa, Osun, Plateau, Yobe and Zamfara) exceeded their respective threshold values. Also all the values of the above threshold values are not equal to the below threshold values,indicating the presence of asymmetric transmission. The presence of asymmetric transmission showed price changes along the chain (from farmer/marketer to consumer) was greater than the market costs of handling cattle and agents will be in a greater position to implement price changes before transmission takes place. This indicates strong asymmetric price transmission since price changes moved from bottom of the chain to the top. There is need to improve on the market information system in the country so that information will flow to all the markets.

Occurrence and risk assessment of pesticides in a Mediterranean Basin with strong agricultural pressure (Guadiana Basin: Southern of Portugal)

The study aimed to assess the occurrence and the environmental risk of a group of 51 selected pesticides in the Guadiana Basin (a biodiversity hotspot, in the Mediterranean). The most abundant pesticides were bentazone and 2,4-D, while terbuthylazine together with terbutryn constituted the most ubiquitous pesticides. Eighteen out of the 38 pesticides detected are no longer approved in Europe, and 5 of them are included in the list of priority substances. The risk assessment showed that azinphos ethyl, diflufenican, irganol, imidacloprid, and oxadiazon occurred occasionally, but always in concentrations above their respective ecotoxicological threshold value. Contrary, bentazone, terbuthylazine, and terbutryn presented a high risk in most of the sampled locations and periods.The site-specific risk assessment showed a spatial and temporal pattern, with a higher risk occurring mainly in intermittent streams, in the drought period. The presence of pesticides banned from the EU market since 2009 showed the importance of improving the monitoring process, to identify the main sources of pollution and the fate of these emerging compounds. The results showed the need of implementing actions to improve the sustainable use of pesticides in agricultural areas, working with farmers and management entities to reduce the contamination of aquatic ecosystems. Transboundary water governance is also required to solve potential transboundary contamination problems.

Odour-active compounds in liquid malt extracts for the baking industry

An odorant screening by gas chromatography–olfactometry (GC–O) and a crude aroma extract dilution analysis (AEDA) applied to the volatiles isolated from a light and a dark liquid malt extract (LME) by solvent extraction and solvent-assisted flavour evaporation (SAFE) identified 28 odorants. Fifteen major odorants were subsequently quantitated and odour activity values (OAVs) were calculated as ratio of the concentration to the respective odour threshold value (OTV). Important odorants in the light LME included 3-(methylsulfanyl)propanal (OAV 1500), (E)-β-damascenone (OAV 430), and 4-ethenyl-2-methoxyphenol (OAV 91). In the dark LME, sotolon (OAV 780), 3-(methylsulfanyl)propanal (OAV 550), (E)-β-damascenone (OAV 410), acetic acid (OAV 160), and maltol (OAV 120) were of particular importance. To get an insight into the changes during malt extract production, the quantitations were extended to the malt used as the starting material for both LMEs. Addition of a minor amount of water to malt before volatile extraction was shown to be effective to cover the free as well as the bound malt odorants. Results showed that some LME odorants originated from the starting material whereas others were formed during processing. Important process-induced LME odorants included (E)-β-damascenone and 4-ethenyl-2-methoxyphenol in the light LME as well as maltol, sotolon, (E)-β-damascenone, and 2-methoxyphenol in the dark LME. In summary, the odorant formation during LME production was shown to be more important than the transfer of odorants from the malt.

Comparison of clinical outcomes associated with arthroscopic cyst wall preservation or resection in the treatment of popliteal cyst: a systematic review and meta-analysis.

Arthroscopy is commonly used to treat popliteal cysts, but the influence of the cyst wall on treatment outcomes remains controversial. The goal of this study was to compare clinical outcomes associated with arthroscopic cyst wall resection versus preservation in patients undergoing treatment for popliteal cysts. We searched the PubMed, Embase, Web of Science, and Cochrane Library databases to identify all relevant articles published as of April 2020. STATA v15.1 was used for all statistical analyses. Relative risk (RR) and corresponding 95% confidence intervals (CIs) pertaining to study outcomes were calculated. Study heterogeneity was evaluated using the I2 statistic and the χ2 test, with I2 > 50% and P < 0.10 as respective significance threshold values. The risk of bias was gauged with the Cochrane Collaboration's risk of bias tool and the Newcastle-Ottawa Scale (NOS). In total, 18 relevant studies were included in this meta-analysis, of which 16 were observational studies and 2 were randomized controlled trials (RCTs). These studies included 573 total patients, of whom 346 underwent arthroscopic cyst resection and 227 underwent arthroscopic cyst preservation. Pooled analyses revealed that clinical outcomes (RR = 0.98, 95% CI 0.94-1.00) and postoperative recurrence rates (RR = 0.90, 95% CI 0.85-0.95) were significantly better among patients that underwent cyst wall resection relative to those that underwent cyst wall preservation (RR = 0, 95% CI 0-0.02 and RR = 0.05, 95% CI 0.02-0.10, respectively). However, complications occurred more often in the cyst wall resection group relative to the cyst wall preservation group (RR = 0.05, 95% CI 0.01-0.12 vs. RR = 0.01, 95% CI 0-0.03). Sensitivity analyses confirmed the stability of these pooled results, and we detected no significant risk of publication bias. Relative to cyst wall preservation, popliteal cyst wall arthroscopic resection can yield more satisfactory clinical results and decrease rates of recurrence, but can also increase the incidence of complications. Future prospective studies comparing the outcomes associated with cyst wall resection and preservation will be required to validate our results.

Velocity-Dependent Perception Threshold for Discrete Imperceptible Repositioning in a Virtual Environment During Eye Blinks

In this study, the relationship between a person’s walking speed and the perception threshold for discrete implicit repositioning during eyeblinks in a virtual environment is investigated. The aim is to estimate the perception thresholds for forward and backward repositioning during forward translation following eyeblink occurrences. A psychophysical method called Staircase Transformed and Weighted up/down is utilized to quantify the perception thresholds for forward and backward repositioning. The perception thresholds for this repositioning are estimated for three different walking speeds: slow (0.58 m/s), moderate (0.86 m/s), and fast (1.1 m/s). The collected observations are then analyzed using regression analysis. The estimated perception threshold values for imperceptible forward repositioning were 0.374, 0.635, and 0.897 meters for the abovementioned walking speeds, respectively. Moreover, the respective perception threshold values for imperceptible backward repositioning were 0.287, 0.430, and 0.572 meters for the same walking speeds. The findings reveal a proportional relationship between the perception threshold values and the participant’s walking speed. As such, it is possible to imperceptibly reposition a participant at a greater distance when they are walking faster relative to the same situation when the participant is walking slower. In addition, the results show that there is more tolerance in forward discrete repositioning compared to backward discrete repositioning during forward translation. These findings enable the extension of the manipulation types utilized by the Redirected Walking Technique. More specifically, this allows for implementing a sophisticated composite redirected walking controller, which utilizes continuous and discrete translation gains simultaneously; this helps not only with reducing the cognitive load, but also with reducing the amount of physical space required to support infinite free exploration in an immersive virtual environment.

Photo-neutron cross-section of $${}^{{\mathrm {nat}}}{\mathrm {Dy}}$$ in the bremsstrahlung end-point energies of 12, 14, 16, 65, and 75 MeV

The flux-weighted average cross-sections of $${}^{\mathrm {nat}}{\mathrm {Dy}}(\upgamma , {\mathrm {xn}}){}^{{159,157,155}}{\mathrm {Dy}}$$ reactions were measured at the bremsstrahlung end-point energies of 12, 14, 16, 65 and 75 MeV with the activation and off-line $$\upgamma $$ -ray spectrometric technique using the 20 MeV Electron Linac for beams with high Brilliance and low Emittance (ELBE) at Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany and the 100 MeV electron linac at the Pohang Accelerator Laboratory, Korea. The $${}^{\mathrm {nat}}{\mathrm {Dy}}(\upgamma , {\mathrm {xn}}){}^{{157,155}}{\mathrm {Dy}}$$ reaction cross-sections as a function of photon energy were also calculated theoretically using TALYS 1.9 code. Then the flux-weighted average values at different end-point energies were obtained based on the theoretical values of mono-energetic photons. These values were compared with the flux-weighted values of present work and are found to be in general agreement. It was also found that the experimental and theoretical formation cross sections of $${}^{159}{\mathrm {Dy}}$$ , $${}^{157}{\mathrm {Dy}}$$ and $${}^{155}{\mathrm {Dy}}$$ from the $${}^{\mathrm {nat}}{\mathrm {Dy}}(\upgamma , {\mathrm {xn}})$$ reactions increased from their respective threshold values to a certain energy where other reaction channels opened. After reaching a maximum value, the individual reaction cross-sections slowly decreased with the increase of the bremsstrahlung energy due to the initiation of other competing reactions at higher energy, which indicates the impact of the excitation energy. However, the production cross sections of $${}^{157}{\mathrm {Dy}}$$ and $${}^{155}{\mathrm {Dy}}$$ from the $${}^{\mathrm {nat}}{\mathrm {Dy}}(\upgamma , {\mathrm {xn}})$$ reactions slightly increase in between and then decreased slowly with bremsstrahlung energy, which is due to the contributing reactions of higher mass isotopes.

Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry

The presence of pharmaceuticals and personal care products (PPCPs) in aquatic systems has raised concern about their potential adverse effects on aquatic organisms. Considering the fact that the physiological/biological effects of PPCPs are triggered when their concentrations in the organism exceeds the respective threshold values, it is important to understand the bioconcentration and toxicokinetics of PPCPs in aquatic organisms. In the present study, we developed a convenient analytical method for the determination of 65 pharmaceuticals and 7 personal care products (log Kow = 0.14–6.04) in plasma and whole-body tissues of fish. The analytical method consists of ultrasound-assisted extraction in methanol/acetonitrile (1:1, v/v,) acidified with acetic acid-ammonium acetate buffer (pH 4), cleanup on a HybridSPE®-Phospholipid cartridge (zirconia-coated silica cartridge), and quantification with liquid chromatography-tandem mass spectrometry (LC–MS/MS). Acceptable accuracy (internal standard-corrected recovery: 70%–120%) and intra- and inter-day precision (coefficient of variation: <15%) were obtained for both plasma and whole-body tissue samples. In addition, low method detection limits were achieved for both plasma (0.0077 to 0.93 ng mL−1) and whole-body tissue (0.022 to 4.3 ng g − 1 wet weight), although the developed method is simple and fast ― a batch of 24 samples can be prepared within 6 h, excluding the time for measurement with LC–MS/MS. The developed method was successfully applied to the analysis of PPCPs in plasma and whole-body tissue samples of fish collected in a treated wastewater-dominated stream, for a comprehensive evaluation of their bioconcentration properties. The analytical method developed in the present study is sufficiently accurate, sensitive, and rapid, and thus highly useful for the comprehensive evaluation of PPCP residues in fish and would aid in future exposome and risk assessment.