Research Expedition Research Articles

Essays of Chokan Valikhanov as a Reflection of the History of Central Asia in the Second Half of the 19th Century

Provides a comprehensive examination of the personal documents of Chokan Valikhanov, reflecting the rich history of Central Asia during the second half of the 19th century. Valikhanov, a prominent Kazakh scholar and explorer, significantly contributed to the understanding of this region through his extensive research and numerous expeditions. The article delves into Valikhanov's biography, highlighting the milestones of his academic journey and the critical impact of his work. Special emphasis is placed on the value of his personal documents in unveiling the ethnographic, geographic, and socio-historical nuances of Central Asia. These documents offer unique insights into the cultural and historical context of the time, providing a deeper understanding of the region’s development. The analysis underscores the scholarly importance of Valikhanov’s findings and their enduring relevance in contemporary studies of Central Asia. Furthermore, the article discusses the methodological approaches used by Valikhanov in his work, noting their innovation and relevance. In conclusion, the significance of Valikhanov's contributions to the historical narrative of Central Asia is affirmed, alongside the potential avenues for future research that his legacy opens up. The enduring value of his personal documents continues to influence modern scholarship, inviting renewed interest and further exploration in the field.

Occurrence, residency, and habitat characterization of leopard seals in Chile

Leopard seals have traditionally been considered Antarctic predators with a Southern Ocean distribution. Historically, sightings north of the Antarctic Polar Front were considered extralimital. However, recent studies suggest a significant presence of leopard seals in subantarctic regions. Here, we assess the spatial occurrence, residency status, and temporal trends of leopard seals in Chile using historical records, stranding reports, standardized monitoring data, photo-identification (photo ID) catalogs, and sightings from four research expeditions. We also characterize glaciers where sightings are concentrated, identifying glaciological and geomorphic attributes that prolong iceberg residency time, which is linked to high leopard seal concentrations. Based on these attributes, we evaluated other potential suitable glacial habitats in Patagonia. We obtained 438 sighting records of leopard seals from 1927 to 2023. Over the last 15 years, we documented a 4-18% annual increase in stranding events reported to national authorities. Most sightings (75%) were concentrated in two hotspots: National Park San Rafael Lagoon, located in Northern Patagonia, and Parry Fjord in Tierra del Fuego. Using photo ID catalogs, we identified 19 resident leopard seals, including 16 multi-year residents observed between 2010-2023 (10 in San Rafael, 6 in Tierra del Fuego) and 3 potential residents (observed multiple months in the same year in Tierra del Fuego). San Rafael monitoring data showed no inter-annual trend, but seasonal trends were observed. We also provide evidence of breeding in Chile, with records of at least 14 pups born and at least two females giving birth in multiple years. Our habitat characterization suggests that calving flux, fjord sinuosity, and fjord width variation are crucial for prolonging iceberg residency in hotspot areas. Based on these attributes, we identified 13 additional fjords in Patagonia as “very likely” suitable for leopard seals. Our study confirms that Patagonia is part of the species’ breeding distribution, shifting the paradigm that leopard seals are merely visitors north of the Antarctic Polar Front. Given the limited number of suitable glaciers in Chile and the potential impacts of climate change, our assessment highlights glacial retreat as a major threat for the ecosystem of this pagophilic marine apex predator in South America.

Investigation of risk factors associated with the development of depressive symptoms in healthy subjects exposed to long-term stress: A prospective study of the Japanese Antarctic research expedition wintering party.

Stressors induce depression together with parenting experienced in childhood, personality traits, and sleep. In this study, we investigated factors associated with the development of depression in a long-term stressful environment, namely, the Antarctic Research Expedition wintering party, by comparing 2 groups, the depression and nondepression groups. A self-administered questionnaire was used to survey 91 members of the Japanese Antarctic Research Expedition who spent winters in the Antarctic base. Psychological evaluations of depression, anxiety, and sleep were performed using a questionnaire every 3 months during the participants' stay in Antarctica. The primary endpoint was the occurrence of minor or major depression, as evaluated by the PHQ-9 score. Participants with a PHQ-9 score of 5 or more during their stay in Antarctica were defined as the depression group (25 subjects), and participants with a PHQ score of 4 or less were defined as the nondepression group (43 subjects). Compared with the nondepression group, the depression group had significantly higher scores for predeparture PHQ-9, state and trait anxiety, sleep disturbance, and neuroticism. Multivariable logistic regression analyses showed that higher predeparture scores of subthreshold depressive symptoms and neuroticism were found to be significant predictors of the occurrence of depression during their stay in Antarctica. This study prospectively showed that subthreshold depressive symptoms and neuroticism, which were suggested as risk factors in previous studies, were confirmed to be risk factors for depression. The results of our study are expected to contribute to the understanding of depression in harsh environments.

Correspondence between Mawson and Simmers: the missing meteorological report of Ritchie Simmers from the BANZARE voyages of 1929–1931 in Antarctic waters

ABSTRACT The British, Australian and New Zealand Antarctic Research Expedition (BANZARE) operated during the 1929–1930 and 1930–1931 austral summers, primarily as a ship-borne expedition in the Southern Ocean. Involvement of meteorologist Ritchie Simmers from the New Zealand Meteorological Service was part of the New Zealand contribution to the expedition. He collected data assiduously, including two-hourly weather observations during the day and 34 balloon flights tracked by theodolite observations. Simmers worked up considerable data but did not produce anything in the BANZARE scientific report series, as expected by Professor Douglas Mawson, series editor and expedition leader. Based on correspondence in the Mawson Centre at the South Australian Museum between Mawson and Simmers about his pending report, we suggest reasons for the striking omission of meteorological information from the report series. Simmers had a busy, full-time career with the New Zealand Meteorological Service, and not until 1947 was he permitted to work on his report in office hours. He was distracted by other events, including studying at Massachusetts Institute of Technology (M.I.T.). We suggest Simmers’ enthusiasm for the project waned after Mawson rebuffed potential collaboration with Professor Rossby at M.I.T. involving BANZARE meteorological data. Little of Simmers’ data set has been located.

The Environmental Impact of a High-Altitude Medical Research Expedition.

Joyce, Kelsey E., Catherine A. Campbell, Arthur R. Bradwell, Samuel J.E. Lucas, Christopher T. Lewis, Rebekah A.I. Lucas, and Mark Edsell. The environmental impact of a high-altitude medical research expedition. High Alt Med Biol. 00:00-00, 2024. Introduction: Scientists must begin examining the environmental cost(s) of their research. The purpose of this study was to evaluate a component of the environmental impact of a high-altitude medical research expedition by totaling the carbon dioxide (CO2) emissions calculated from as many direct and indirect sources as possible. Methods: Eighteen individuals flew from London to Bagdogra (via Delhi), and then drove onward to Lachung (via Gangtok) where they began their ascent on foot to 4,800 m (Kanchenjunga National Park, Sikkim). Several research experiments were conducted throughout the expedition, which required use of a laboratory centrifuge, solid CO2 (specimen storage), rechargeable laptop computers and battery-powered oximeters. International Civil Aviation Organization calculators estimated aviation CO2 production. Land emissions were calculated for Mahindra vehicles. Solid waste was weighed and CO2 emissions estimated for its incineration. Results: Total CO2 emissions equated to ∼16.7 tonnes from the following sources: air and land transportation of expedition team (87.3%); sublimation and transportation of solid CO2 (7.7%), waste incineration (0.58%), generator transportation and gasoline (12 l) combustion (0.48%), and battery transportation (3.3%). Conclusions: Air travel contributed the most to the overall environmental cost of the research expedition. Further investigation is required to contextualize these findings in relation to lab-based alternative(s).

Conspectus of the vegetation types of Tajikistan and adjacent areas with special focus on phytosociological classes

Vegetation classification is a tool for organizing the patterns and diversity of plant communities. It is used in nature conservation, and helps to understand the role of vegetation in the biosphere. Given the rapidly diminishing opportunity to understand the vegetation diversity of Tajikistan located in the Middle Asian region, phytosociological surveys using the floristic-ecological approach and the Braun-Blanquet cover-abundance scale were undertaken in 2006. Aer 18 years of research, with a total of 55 research expeditions to Tajikistan, Kyrgyzstan, and Uzbekistan, it is time to summarise and make a first attempt to establish a comprehensive classification system for the entire vegetation of Tajikistan and the surrounding areas. As a first step, the Vegetation of Middle Asia (VMA) database with records from three countries: Tajikistan (4,130 relevés), Kyrgyzstan (1,681 relevés) and Uzbekistan (13 relevés) was created. e internal comparison of all the plots and the analysis of the similarities with the vegetation of Iran, Russia, Afghanistan, Mongolia, Pakistan, and China make it possible to present the comprehensive list of plant communities organized in 45 classes, 47 orders, 83 alliances, 297 associations, and 237 rankless units. In addition to those described in our previous works, we propose also several new sytaxa: <i>Eremogono griffithii-Nepetetea podostachyos, Artemisio persicae-Stipetea drobovii, Festucetea alaico-krylovianae, Carici koshewnikowii-Asperuletea oppositifoliae, Trichodesmo incani-Pachypterygietea brevipes, Aceretalia turkestanici, Juniperetalia seravschanicae, Crataegetalia ponticae, Populetalia pruinoso-euphraticae, Salici capusii- Hippophaeetalia rhamnoidis, Carici stenophylloidis-Stipetalia drobovii, Eritrichion pamirico-subjacquemontii, Stipetum kazachstanicae, Caricetum pamirensis</i>, and <i>Bolboschoenetum affinis</i>. Tis systematic compilation of data, based on our fieldwork and literature data, is not definitive, and certainly the rich and diverse vegetation of Middle Asia requires further studies incorporating also modern LIDAR and satellite techniques.

Advancing Community Curation of Research Expeditions: A Collaborative Journey with Wikidata and Biodiversity Information Standards

Advancing Community Curation of Research Expeditions: A Collaborative Journey with Wikidata and Biodiversity Information Standards

Latitudinal variation in zooplankton over the Emperor Seamounts (34°–44° N, 170°–171° E) during the summer of 2019

During a research expedition to the Northwest Pacific between July 13 and August 11, 2019, we collected oceanic zooplankton samples over the Emperor Seamounts (Koko, Jingu, Nintoku, and Suiko guyots). We analyzed the horizontal and vertical distributions of abundance and biomass and also the structure of the zooplankton in the highly productive but poorly studied ocean waters between 34° and 44° N. The zooplankton was represented by four genera of amphipods, seven genera of pteropods, 39 genera of copepods, and larvae of benthic invertebrates and fish. The abundance and biomass of zooplankton increased towards higher latitudes along the series Koko < Jingu < Nintoku < Suiko guyots. The highest index of diversity was recorded over the Koko and Jingu guyots; the highest species richness occurred over the Jingu and Nintoku guyots. Small-sized copepods, appendicularians, chaetognaths, euphausiids, and fish eggs and larvae concentrated in the epipelagic; large copepods and ostracods concentrated in the mesopelagic. We identified three types of zooplankton assemblages: a Subtropical assemblage between 34°–35° N characterized by tropical/subtropical forms; a Transition assemblage characterized by subtropical, subarctic, and widespread species between 38°–41° N; and a Subarctic assemblage characterized by subarctic and widespread species between 43°–44° N. The spatial distribution of zooplankton was a function of environmental variables such as surface salinity, temperature at 200-m, and, to a certain extent, by surface Chl-a concentrations.

Comparative analysis of fisheries and biological characteristics of bream (Abramis brama) in Saratov and Volgograd reservoirs

Based on the results of ichthyological surveys and industrial catch data, a comparative analysis of the commercial biological characteristics of bream in the Saratov and Volgograd reservoirs was carried out. The material was the results of expeditionary research by employees of the Saratov branch of the VNIRO. With the existing fishery, bream stocks are in a stable condition. The volumes of commercial stocks and catches of the species in question in the Volgograd Reservoir are higher than in the Saratov Reservoir. Despite differences in fishing intensity and characteristics of reservoirs, the size and weight indicators of the species are similar.

The influence of heavy shipping traffic on the structure and dynamics of sea ice in the southwestern Kara Sea

The development of the Northern Sea Route and the beginning of year-round transit shipping require not only the production of new icebreakers and ice-class vessels, but also the development of a specialized hydrometeorological support system for ice shipping. For the analysis of satellite data and the development and validation of ice forecasts, actual field data on the ice cover is required. This data can only be obtained from shipboard observations; however, scientific expeditions are rarely organized during the winter. In order to obtain new data over the area of intensive shipping, two expeditions were organized on board of nuclear icebreakers in the southwestern Kara Sea (“LED-SMP” expeditions) in 2023 and 2024. Specialized hydrometeorological maintenance of ice shipping in the southwestern Kara Sea together with the research expeditions “LED-SMP” carried out in the same place and time on board the nuclear icebreakers revealed the influence of the technogenic factor on the sea ice structure and dynamics was revealed. In winter and spring, two main routes are used for navigation in the southwestern Kara Sea: through the Kara Gate Strait and north of Cape Zhelaniya. In April 2024, a unique situation occurred when, due to the difficult ice conditions east of the Kara Gate Strait, the entire ship traffic was directed north of Cape Zhelaniya. In preparing a long-term ice forecast, it was noted that after the redirection the natural development of ice processes changed. At the beginning of the winter period 2024,

Warming events and their causes at the Bering Sea section B in summer of 1999–2019

Based on hydrological data for the Bering Sea from 1999 to 2019 during the Chinese National Arctic Research Expeditions (CHINAREs), along with oceanographic and meteorological data from the National Centers for Environmental Information (NCEI) and the European Centre for Medium-Range Weather Forecasts (ECMWF), we investigated trends in summer at the Bering Sea section B over the past 20 years and their underlying causes. The results revealed a pronounced rise in temperature at the Bering Sea section B in 2003, 2014, 2016, 2018, and 2019, suggesting a shift towards a new phase of warmth starting in 2014. In warm years (2003, 2014, 2016, 2018, and 2019), the mean maximum temperature in the upper layer in the basin north of 57°N and on the continental shelf were roughly 2.7 °C, 2.6 °C higher than that in cold years (1999, 2008, 2010, and 2012). Additionally, the mean minimum temperature of the cold water in the middle layer in the basin was roughly 0.7 °C higher in the warm years compared to the cold years. This difference between the warm and cold years was evident in the heat content: the average heat content in the upper to middle layers in the basin north of 57°N and on the continental shelf were about 1.2 and 0.9 GJ/m2 higher, respectively, in the warm years than the cold years. However, there was a modest warming trend in the basin south of 57°N, and the temperature in the mid-layer on the continental shelf showed year-to-year stability, in contrast to the significant warming observed elsewhere. Further study suggested that temperature fluctuation in the basin was closely tied to seasonal 2-m air temperature. In addition, the southern basin, divided at 57°N, experienced larger positive or negative anomalous wind stress curl compared to the northern basin. This discrepancy resulted in differing causes of temperature variation between the two regions. Temperature change in the upper layer on the continental shelf was attributed to an increase in cumulative net heat flux resulting from reduced sea ice, while the temperature in the middle layer on the continental shelf was limited by the enhanced density gradient between the upper and middle layer caused by melt ice, preventing significant warming. Overall, the thermal state for the Bering Sea section B was influenced by a combination of factors, including net heat flux, sea ice, and atmospheric conditions, contributing to the year-to-year variation characterized by alternating cold and warm regimes.

AKMA3 Cruise Report

The AKMA3 oceanographic expedition (29th April–10th May) is part of the Advancing Knowledge of Methane in the Arctic, a project funded by the Norwegian Research Council (287869). The main aim of AKMA is to develop a long-term, multidisciplinary education, and research collaboration focused on Arctic methane sources, processes, ecosystems, and geological history to provide exceptional training for the next generation of experts in Arctic marine sciences and greenhouse gas phenomena. The AKMA3 oceanographic expedition focuses on three objectives: SCIENCE: Focus on the interplay between changing ocean conditions and the physical, biological, and chemical response of extreme environments (methane and oil seepage sites) through a multidisciplinary study of sites in the Barents Sea. Research activities: study sediment-sea-air greenhouse gas exchange; conducting water-column profiling of CH4; gas source analyses; biological and sediment sampling. EDUCATION: This high-level science on this cruise also includes sea-going training for students to develop their knowledge in collecting, curating, and processing samples and data, providing opportunities to fully engage in marine science. OUTREACH: To generate enthusiasm and interest in research expeditions with students and to promote a deeper understanding of science and its applications.

THERAPEUTIC POTENTIAL OF BHANGA IN KASHTARTAVA: A LITERATURE REVIEW

Kashtartava (Dysmenorrhea) is a prevalent co-morbidity in women. The name "Kashtartava" might be inferred as the condition in which Artava sheds pain, which can be associated with Dysmenorrhea. Around the world, 50-90% of women in their reproductive years report having painful periods, a disorder known as Dysmenorrhea. In this review article, we delved into the traditional uses, pharmacological properties and challenges associated with using Bhanga (Cannabis sativa Linn) for managing Kashtartava and its related effects. Research expedition sheds light on the potential therapeutic benefits, mainly focusing on its active component, tetrahydrocannabinol (THC), which exhibits analgesic, anti-inflammatory, antiemetic, and neuroprotective properties. This review highlights the challenges in conducting rigorous research on using the herb during menstruation and pregnancy, owing to study design limitations and confounding variables. This legal ambiguity underscores the importance of understanding and addressing the cultural and legal context surrounding its use in contemporary healthcare prac-tices. Moreover, the psychoactive nature of THC raises concerns regarding its impact on mood and cognition, warranting cautious consideration in clinical applications.

Diversity, toxicity, and distribution of potentially toxic diatoms in Antarctic waters––With description of Pseudo-nitzschia meridionalis sp. nov. and P. glacialis sp. nov.

Diatoms of the genus Pseudo-nitzschia, known for their potential toxicity, are integral to the phytoplankton community of the Southern Ocean, which surrounds Antarctica. Despite their ecological importance, the diversity and toxicity of Pseudo-nitzschia in this region remain underexplored. Globally, these diatoms are notorious for forming harmful algal blooms in temperate and tropical waters, causing significant impacts on marine life, ecosystems, and coastal economies. However, detailed information on the diversity, morphology, and toxicity of Pseudo-nitzschia species in Antarctic waters is limited, with molecular characterizations of these species being particularly scarce.During three research expeditions to the Southern Ocean, monoclonal strains of Pseudo-nitzschia were isolated and cultivated. Stored samples from a fourth expedition, the Brategg expedition, were used to complete the description of particularly P. turgidula. Through electron microscopy and molecular analysis, two novel species were identified—Pseudo-nitzschia meridionalis sp. nov. and Pseudo-nitzschia glacialis sp. nov.—alongside the previously described species P. subcurvata, P. turgiduloides, and P. turgidula. Toxin assays revealed no detectable levels of domoic acid in P. turgiduloides, P. turgidula, P. meridionalis sp. nov. and P. glacialis sp. nov. Conversely, P. subcurvata was reported in a related study to produce domoic acid and its isomer, isodomoic acid C.These findings emphasize the need for comprehensive research on the phytoplankton of Antarctic waters, which is currently a largely uncharted domain. With the looming threat of climate change, understanding the dynamics of potentially harmful algal populations in this region is becoming increasingly critical.

Fungal aerosols over the Northwest Pacific to Arctic Ocean: The influence of air mass and environmental factors

Bioaerosols, prevalent in the atmosphere, serve as carriers for microbe transmission and can also act as Cloud Condensation Nuclei (CCN) and Ice Nuclei (IN), exerting significant influence on cloud microphysics, radiative forcing, precipitation processes, and global climate dynamics. In this study, we conducted a comprehensive study of airborne fungi from the Northwest Pacific to the Arctic Ocean. During the 9th Chinese National Arctic Research Expedition (Chinare, 2018) cruise, total suspended particulate matter (TSP) samples were collected, followed by high-throughput sequencing to determine the microbial community's structure. Ascomycota and Basidiomycota are widespread in marine fungal aerosols along this route, with a notably higher abundance of Epicoccum and Cladosporium in fungal aerosols from the low-latitude Northwest Pacific compared to the high-latitude Arctic Ocean region. The composition of fungal communities depended on air mass transport processes and environmental factors. Long-range transport of terrestrial air masses not only enriches the abundance of fungal aerosols but also increases the relative abundance of dominant genera such as Basidiomycota, Epicoccum and Cladosporium. The knowledge gap about fungal aerosols over the Arctic route from the Northwest Pacific to the Arctic Ocean was filled in part by this work. This study deepens our understanding of how environmental factors and terrestrial air masses influence the composition and distribution of fungal community, offering valuable insights into the interconnections between marine and terrestrial ecosystems and their potential impacts on climate and atmospheric processes.

Ensemble modelling to predict the distribution of vulnerable marine ecosystems indicator taxa on data‐limited seamounts of Cabo Verde (NW Africa)

AbstractAimSeamounts are conspicuous geological features with an important ecological role and can be considered vulnerable marine ecosystems (VMEs). Since many deep‐sea regions remain largely unexplored, investigating the occurrence of VME taxa on seamounts is challenging. Our study aimed to predict the distribution of four cold‐water coral (CWC) taxa, indicators for VMEs, in a region where occurrence data are scarce.LocationSeamounts around the Cabo Verde archipelago (NW Africa).MethodsWe used species presence–absence data obtained from remotely operated vehicle (ROV) footage collected during two research expeditions. Terrain variables calculated using a multiscale approach from a 100‐m‐resolution bathymetry grid, as well as physical oceanographical data from the VIKING20X model, at a native resolution of 1/20°, were used as environmental predictors. Two modelling techniques (generalized additive model and random forest) were employed and single‐model predictions were combined into a final weighted‐average ensemble model. Model performance was validated using different metrics through cross‐validation.ResultsTerrain orientation, at broad scale, presented one of the highest relative variable contributions to the distribution models of all CWC taxa, suggesting that hydrodynamic–topographic interactions on the seamounts could benefit CWCs by maximizing food supply. However, changes at finer scales in terrain morphology and bottom salinity were important for driving differences in the distribution of specific CWCs. The ensemble model predicted the presence of VME taxa on all seamounts and consistently achieved the highest performance metrics, outperforming individual models. Nonetheless, model extrapolation and uncertainty, measured as the coefficient of variation, were high, particularly, in least surveyed areas across seamounts, highlighting the need to collect more data in future surveys.Main ConclusionsOur study shows how data‐poor areas may be assessed for the likelihood of VMEs and provides important information to guide future research in Cabo Verde, which is fundamental to advise ongoing conservation planning.

Transcriptome sequencing of seven deep marine invertebrates

We present 4k video and whole transcriptome data for seven deep-sea invertebrate animals collected in the Eastern Pacific Ocean during a research expedition onboard the Schmidt Ocean Institute’s R/V Falkor in August of 2021. The animals include one jellyfish (Atolla sp.), three siphonophores (Apolemia sp., Praya sp., and Halistemma sp.), one larvacean (Bathochordaeus mcnutti), one tunicate (Pyrosomatidae sp.), and one ctenophore (Lampocteis sp.). Four of the animals were sequenced with long-read RNA sequencing technology, such that the reads themselves define a reference assembly for those animals. The larvacean tissues were successfully preserved in situ and has paired long-read reference data and short read quantitative transcriptomic data for within-specimen analyses of gene expression. Additionally, for three animals we provide quantitative image data, and a 3D model for one siphonophore. The paired image and transcriptomic data can be used for species identification, species description, and reference genetic data for these deep-sea animals.

Methane plume detection after the 2022 Nord Stream pipeline explosion in the Baltic Sea

On September 26th, 2022, the detonations at the gas pipelines Nord Stream 1 and 2 resulted in some of the largest non-natural releases of methane known. The distribution of methane in the surrounding seawater and the possible effects were not apparent. To trace the pathways of methane we recorded CH4 concentrations and the isotopic signal (δ13C-CH4) in seawater, and air. A week post-explosion, we detected methane concentrations up to 4 orders of magnitude above the natural Baltic Sea background. The released fossil methane created a distinct plume with δ13C-CH4 ratios differing from natural background values. The strong water stratification preserved the distribution pattern initiated by the explosion, shown by the laterally strong concentration gradient within the plume. Our analysis encompasses three stages of the explosion's impact; the initial sea-air methane release, measurements taken during our research expedition one week later, and a third stage triggered by the shift from summer to winter conditions as an outlook on how winter mixing and microbial activity will influence the plume.

REGIONAL FLORISTIC RARITIES OF POLTAVA REGION: ECOLOGICAL- PHYTOCENOTIC CHARACTERISTICS, DISTRIBUTION AND CONSERVATION STRATEGIES

The study provides an eco-phytocenotic characterization and assessment of regional floral rarities in the Poltava region. The distribution conditions of these rarities are considered, along with proposed strategies for conserving and protecting valuable plant species. The research results are important for understanding the region’s biodiversity and developing conservation programs for natural ecosystems.The results of the expeditionary research conducted by the authors from 2006 to 2023 indicate that there are 12 species of rare flora in the studied region. Of these, 7 species are forest-dwelling, 1 is found in marshes, and 4 are characteristic of salt marshes and saline meadows. Four species grow at the western and southern borders of their range in the Poltava region two at the eastern border, and two species more are represented by isolated localities. These species require special protection and attention, making it crucial to preserve all identified localities of these rare species, investigate the condition of their populations, and monitor their status in the future.

Contributions of Griffith Pugh to Success on Mt. Everest and His Impact on the Advancement of Altitude and Environmental Physiology.

Griffith Pugh, MD (1909-1994), was a pioneer in altitude physiology. During World War II, he developed training protocols in Lebanon to improve soldier performance at altitude and in the cold. In 1951 he was chosen to join the British Everest team as a scientist. In preparation, he developed strategies for success on a training expedition on Cho Oyu in 1952. Results from Cho Oyu led to the use of supplemental oxygen at higher flow rates during ascent than used previously (4 L/min vs 2 L/min) and continued use (at a reduced rate of 2 L/min) during descent, enabling increased performance and improved mental acuity. Oxygen was also used during sleep, leading to improved sleep and warmth. Adequate hydration (∼3 L/day) was also stressed, and a more appealing diet led to improved nutrition and condition of the climbers. Improved hygiene practices and acclimatization protocols were also developed. These strategies contributed to the first successful summiting of Mount Everest in 1953. Pugh was then appointed as the lead scientist for a ground-breaking eight-and-a-half-month research expedition where the team was the first to overwinter at high altitude (5800 m) in the Himalayas. This current work summarizes Pugh's scientific contributions as they relate to success on Mount Everest and in inspiring future altitude research by generations of successful researchers.