Reproductive Development Research Articles

Emerging roles and mechanisms of lncRNAs in fruit and vegetables.

With the development of genome sequencing technologies, many long non-coding RNAs (lncRNAs) have been identified in fruit and vegetables. lncRNAs are primarily transcribed and spliced by RNA polymerase II (PolII) or plant-specific PolIV/V, and exhibit limited evolutionary conservation. lncRNAs intricately regulate various aspects of fruit and vegetables, including pigment accumulation, reproductive tissue development, fruit ripening, and responses to biotic and abiotic stresses, through diverse mechanisms such as gene expression modulation, interaction with hormones and transcription factors, microRNA regulation, and involvement in alternative splicing. This review presents a comprehensive overview of lncRNA classification, basic characteristics, and, most importantly, recent advances in understanding their functions and regulatory mechanisms.

The Phenotypic Diversity of 232 Germplasm Accessions Identifies the Adverse Effects of Flowering Redundancy on Peanut Yield

Peanut is a leguminous crop with an indeterminate growth habit that will continuously flower during the entire reproductive development stage. Flowering redundancy adversely affects the yield and quality of peanut. In this study, eight flowering and five yield-related traits were collected and comprehensively evaluated from a diverse germplasm panel consisting of 232 peanut accessions, aiming to provide a theoretical basis for improving the flowering habit and yield for future peanut breeding efforts. As a result, large phenotypic diversity was observed in 13 traits. Most of the traits suggested high heritability, except high effective flowering duration (HEFD), days to 100% flower cessation (DTC100), and yield per plant (YPP). Days to 90% flower cessation (DTC90), days to DTC100, flowering duration (FD), and low effective flowering duration (LEFD) showed significant negative correlations with 100-seed weight, 100-pod weight, YPP, average weight per pod, and shelling percentage. Principal component analysis (PCA) suggested that flowering redundancy traits as well as yield-related traits contributed more to the first three PCs when compared to other traits. This study addresses the lack of peanut flowering phenotypic and genetic diversity and lays the foundation for in-depth research on redundancy-related genes in peanut flowering.

Discovery of a novel miRNA involved in the regulation of male infertility in zebrafish

Azoospermia and asthenospermia are common manifestations of male infertility, but it needs further studies to understand the intrinsic regulation mechanism. As a popular model organism, zebrafish is often used to assess reproductive complications. In this study, by analyzing miRNA transcriptome of the mature triploid zebrafish testis afflicted with spermatogenic dysfunctions, leading to the identification of 36 miRNAs that are differentially expressed in comparison with diploid, which are predicted to target 2737 genes. Subsequent functional annotation of these genes pinpointed two miRNAs might association with spermatogenesis. Inhibitory experiments showed that NC_007115.7.7_998413 inhibited conducts a substantial decline in sperm density, and conducted lower embryo fertilization rate than control. And putative target genes qRT-PCR evaluation showed that spata2 was significant down-regulate upon inhibited NC_007115.7.7_998413. In summary, this research positions newly identified miRNA NC_007115.7.998413 as a regulatory factor in male zebrafish reproductive development, enhancing our comprehension of the molecular regulated pathways involved in spermatogenesis.

Abundance of Vibrio aestuarianus, water temperature, and stocking density are associated with summer mortality of Pacific oysters in suspended culture

High mortality rates of cultured Pacific oysters (Crassostrea gigas) during the summer months have regularly occurred on oyster farms in British Columbia, Canada over the last 10 years, but little is known about the microbial and environmental conditions that contribute to such mortality events. The objective of the study was to determine correlative factors associated with the onset of a summer mortality event in oysters (mean ± SD shell height: 14.2 ± 0.5 mm) grown in suspended culture at four stocking densities (150, 300, 450, 600 oysters tray−1) from May 11 to September 17, 2018. Variables examined included both biotic (oyster growth, mortality, reproductive development, and microbiome (approximately every week); Vibrio and harmful algal species abundance) and abiotic (temperature, salinity, turbidity, dissolved oxygen, pCO2, pH, and aragonite saturation) ones. Both the absolute abundance of V. aestuarianus and the relative abundance of Vibrio spp. increased with observed oyster mortality and declining health. Mortality was highest on August 12 and associated with a prior period of elevated temperatures (i.e., increasing temperatures from early July to early August) and increased oyster growth/reproductive development. At that time, systemic mixed microbial infections and necrotic gill tissue in histological cross sections were observed in 19% of oysters that appeared healthy macroscopically. Cumulative percent mortalities per tray ranged from 34 to 75%, the highest-density trays having significantly less mortality and smaller shell width, shell length, and gonad length than lower-density trays. This study demonstrates the significant impact of summer mortality on Pacific oysters and highlights the biotic (host growth, reproductive development, and microbiome composition as well as Vibrio spp. abundance) and abiotic (water temperature) factors associated with the observed mortality in this region.

Reproductive development of male lambs born to undernourished mothers supplemented or not with concentrate during gestation

Southern Patagonia offers harsh environmental and nutritional conditions for sheep pregnancy, which may compromise the fetal and postnatal development of lambs. The aim of the study was to compare the sexual behavior and attractiveness, and semen production and quality during the first year of life of ram lambs born to undernourished mothers grazing on natural pastures, supplemented or not with a concentrate. The study was performed under extensive Patagonian conditions, and a supplement was provided from day 44 of gestation until lambing. Twelve ram lambs born to supplemented ewes (group Sup) and 12 born to control underfed ewes (group Con) were used. Both groups of male lambs remained in a common paddock before and after weaning at 4 months-old. Sexual behavior and attractiveness, and semen production and quality of ram lambs were evaluated when they were 6 and 12 months-old. Sup lambs were heavier than Con at birth, and tended to maintain this difference when they were 4 mo-old; afterward, this difference disappeared. At 6 months-old, Con ram lambs displayed greater sexual activity when individually exposed to an estrous ewe, as well as a trend to a greater sperm concentration. Differences between groups in sexual behavior decreased at 12 months-old, and no differences were detected in seminal traits. In conclusion, supplementation of undernourished pregnant ewes with concentrate from mid-pregnancy until lambing slightly impacted the progeny reproductive performance when they were 6 months-old, an effect that was attenuated at 12 months-old. The effects of the nutritional status of pregnant ewes on the reproductive development of the offspring likely were compensated by a greater growth rate during early life before puberty.

Parasitizing efficiency of Tetrastichus howardi (Olliff) (Hymenoptera: Eulophidae) on Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) larva and pupa

BackgroundThis study evaluated the parasitizing efficiency of Tetrastichus howardi (Olliff) (Hymenoptera: Eulophidae) on the Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) larvae and pupae. The sixth instar larvae and 48 h. old pupae were individually exposed for 24 h. to 15 newly emerged mated females of T. howardi. Following the emergence of parasitoids, observations were recorded on the percentage of parasitized and emergent pupae and larvae, life cycle duration, progeny, male and female emergence, sex ratio, longevity of male and female and adult per mg weight.ResultsIn both pupal and larval, parasitization and emergence were 100%. The pupa produced 438.56 progenies, with 394.76 females and 43.2 males and a sex ratio of 0.89. In larvae, 311.93 progenies emerged, with 259.83 females and 53.0 males and a sex ratio of 0.83. The T. howardi life cycle duration was 17.66 days in pupae and 20.13 days in larvae. Longevity of female and male progeny that emerged from pupae and larvae was 15.83, 13.40, and 10.40, 8.76 days respectively. The overall progeny production in pupae and larvae was 2.75 and 1.60 individuals per mg body weight, respectively.ConclusionsThe highest parasitism as well as biological and reproductive development of T. howardi in G. mellonella pupae and larvae suggests that this host could be employed as an alternate host for mass multiplication.

Single-cell transcriptome profiling implicates the psychological stress-induced disruption of spermatogenesis

Male infertility has emerged as a global issue, partly attributed to psychological stress. However, the cellular and molecular mechanisms underlying the adverse effects of psychological stress on male reproductive function remain elusive. We created a psychologically stressed model using terrified-sound and profiled the testes from stressed and control rats using single-cell RNA sequencing. Comparative and comprehensive transcriptome analyses of 11,744 testicular cells depicted the cellular landscape of spermatogenesis and revealed significant molecular alterations of spermatogenesis suffering from psychological stress.At the cellular level, stressed rats exhibited delayed spermatogenesis at the spermatogonia and pachytene phases, resulting in reduced sperm production. Additionally, psychological stress rewired cellular interactions among germ cells, negatively impacting reproductive development. Molecularly, we observed the down-regulation of anti-oxidation-related genes and up-regulation of genes promoting reactive oxygen species (ROS) generation in the stress group. These alterations led to elevated ROS levels in testes, affecting the expression of key regulators such as ATF2 and STAR, which caused reproductive damage through apoptosis or inhibition of testosterone synthesis. Overall, our study aimed to uncover the cellular and molecular mechanisms by which psychological stress disrupts spermatogenesis, offering insights into the mechanisms of psychological stress-induced male infertility in other species and promises in potential therapeutic targets.

Women decision making on use of modern family planning methods and associated factors, evidence from PMA Ethiopia.

Family planning decision making is defined as women´s ability to determine the family planning methods that she wanted to use through the process of informed decision making. Despite the availability and accessibility of family planning methods, the utilization rate is not more than 41% in Ethiopia. Evidence and experts have consistently show that women decisions making ability on family planning method they desired to use is one of the possible reasons for this slow rate of family planning use increment. In consideration of this and further motives family planning use decision making has become one of the top sexual and reproductive health related sustainable development agendas. Hence, this study aimed at determining the level, trend and spatial distribution of family planning use decision making among married women and identify factors affecting it. This study was based on Performance Monitoring for Action (PMA) 2020 cross sectional national survey data. Married women who are currently using or recently used family planning method were included in this study. Frequency was computed to describe the study participants while chi-square statistics was computed to examine the overall association of independent variable with family planning use decision making. To identify predictors of family planning use decision making multinomial logistics regression was employed. Results were presented in the form of percentage and relative risk ratio with 95% CI. Candidate variables were selected using p value of 0.25. Significance was declared at p value 0.05. This study revealed that one in two women (51.2%; 95% CI: 48.8%-53.6%) decide their family planning use by themselves while 37% (36.8%; 95% CI: 34.5%-39.2%) decide jointly with their husband and/or partner. Women alone family planning use decision making increased significantly 32.8% (95% CI: 29.4%, 36.4%) in 2014 to 51.2% (95% CI: 48.8%, 53.6%) in 2020. It also shows variation across regions from scanty in Afar and Somali to 63.6% in Amhara region and 61.5 Addis Ababa. Obtaining desired family planning method was found significantly to improve women alone and joint family planning use decision making. Women who have perceive control and feeling if they get pregnant now were found to be positively associated with women alone family planning use decision making. Discussion with husband, his feeling towards family planning were found positively to influence family planning use joint decision making. Moreover, women religion, was found reducing the likelihood of both women alone and joint family planning use decision making while experiencing side effect reduces the likelihood of joint family planning use decision making. Half of the women independently decide their family planning use which calls up on further improvement. Family planning use decision making ability is expected to be improved by efforts targeted on husbands' approval on wife's family planning use, discussion on family planning use with husband/partner, improving women psychosociological readiness and trust on her own to decide her desired family planning method; informing the possible side effects and what to do when they encountered during their family planning use visit. In addition, influencing women on the use of family planning via religious leader will help much in this regard. Monitoring and evaluating reproductive health policy 2021 to2025 and addressing bottlenecks which hinder women decision making health service use is hoped to improve women family planning use decision making. Further qualitative study to identify and address factors that contribute for the variation across regions also help much.

Climate-smart cotton (Gossypium herbaceum) crop production in Punjab: A comprehensive review of sustainable management practices

The growth and productivity of cotton (Gossypium herbaceum L.) crop are greatly influenced by major fluctuations in the local weather. The output of cotton is negatively impacted by extended periods of unfavourable weather, despite the crop's apparent relative resistance to heat and drought. Among various cotton growing states in India, Gujarat often leads as the highest cotton-producing state in the country followed by Maharashtra, Telangana, Rajasthan, Karnataka and Andhra Pradesh. These states collectively contribute more than 65% to India's cotton production. In Punjab, the optimal sowing window for cotton spans from the 1stApril to 15th May. Deviating from this recommended time frame, be it through excessively early or late sowing, correlates with compromised crop establishment and reduced productivity. Temperature thresholds dictate progression of the cotton crop's key stages. For germination, a threshold temperature of 16°C is required, while the range of 21 to 27°C is conducive to vegetative growth, and 27 to 32°C supports reproductive development. The diurnal/night time temperature for fostering cotton root growth resides within 30°C/22°C to 35°C/27°C. Elevated temperatures (40°C/32°C), even under adequate water and nutrient conditions, results in the emergence of shallow root systems. Strategic measures encompassing suitable sowing dates, precise plant spacing, favourable row orientations, judicious irrigation techniques and mulching can effectively temper the influence of climate change on cotton output. Furthermore, the application of crop simulation models and access to timely, accurate weather forecasts and agro-advisories equips decision-makers with invaluable insights for short-term operational planning.

The transcription factors and pathways underpinning male reproductive development in Arabidopsis.

As Arabidopsis flowers mature, specialized cells within the anthers undergo meiosis, leading to the production of haploid microspores that differentiate into mature pollen grains, each containing two sperm cells for double fertilization. During pollination, the pollen grains are dispersed from the anthers to the stigma for subsequent fertilization. Transcriptomic studies have identified a large number of genes expressed over the course of male reproductive development and subsequent functional characterization of some have revealed their involvement in floral meristem establishment, floral organ growth, sporogenesis, meiosis, microsporogenesis, and pollen maturation. These genes encode a plethora of proteins, ranging from transcriptional regulators to enzymes. This review will focus on the regulatory networks that control male reproductive development, starting from flower development and ending with anther dehiscence, with a focus on transcription factors and some of their notable target genes.

Sex‐specific gene expression in eastern oyster, Crassostrea virginica, gonad and mantle tissues

AbstractThe eastern oyster (Crassostrea virginica) is a protandrous hermaphrodite of commercial importance. As with many marine invertebrates, little is known about sex determination and differentiation systems in this species. Such knowledge has important implications not only for understanding the evolution of sex but also for applied questions in aquaculture. In order to examine mechanistic differences in reproductive development between the sexes, we compared the transcriptomes of gonad and mantle tissues from six male and six female oysters. A total of 7675 transcripts were differentially expressed between male and female gonads (3936 and 3739 were upregulated in males and females, respectively). Transcripts identified include those associated with sex in other invertebrate and vertebrate species such as Dmrt1, Sox‐30, Bindin, Dpy‐30, and Histone H4 in males and Foxl2, Vitellogenin, and Bystin in females. GO terms associated with transcripts upregulated in male gonads include protein modification, reproductive process, and cell projection organization, whereas RNA metabolic process and amino acid metabolic process were associated with transcripts upregulated in females. Far fewer transcripts were differentially expressed between male and female mantle tissues, with 87 transcripts upregulated in females and 16 upregulated in males. However, 41% of transcripts identified as differentially expressed between mantle tissues were also differentially expressed between male and female gonads including Histone H4 and Bystin. This study represents the first characterization of eastern oyster male and female gonad transcriptomes. We further identify differing expression profiles between male and female mantle tissues, which provides evidence for sex‐specific functions of the mantle and suggests that this tissue could harbor biomarkers for identifying oyster sex non‐destructively.

Benzodiazepine Interference with Fertility and Embryo Development: A Preliminary Survey in the Sea Urchin Paracentrotus lividus.

Psychotropic drugs and benzodiazepines are nowadays among the primary substances of abuse. This results in a large and constant release into aquatic environments where they have potentially harmful effects on non-target organisms and, eventually, human health. In the last decades, evidence has been collected on the possible interference of benzodiazepines with reproductive processes, but data are few and incomplete. In this study, the possible negative influence of delorazepam on fertilization and embryo development has been tested in Paracentrotus lividus, a key model organism in studies of reproduction and embryonic development. Sperm, eggs, or fertilized eggs have been exposed to delorazepam at three concentrations: 1 μg/L (environmentally realistic), 5 μg/L, and 10 μg/L. Results indicate that delorazepam reduces the fertilizing capacity of male and female gametes and interferes with fertilization and embryo development. Exposure causes anatomical anomalies in plutei, accelerates/delays development, and alters the presence and distribution of glycoconjugates such as N-Acetyl-glucosamine, α-linked fucose, and α-linked mannose in both morulae and plutei. These results should attract attention to the reproductive fitness of aquatic species exposed to benzodiazepines and pave the way for further investigation of the effects they may exert on human fertility. The presence of benzodiazepines in the aquatic environment raises concerns about the reproductive well-being of aquatic species. Additionally, it prompts worries regarding potential impacts on human fertility due to the excessive use of anxiolytics.

Identifying long non-coding RNAs involved in heat stress response during wheat pollen development.

Wheat is a staple food crop for over one-third of the global population. However, the stability of wheat productivity is threatened by heat waves associated with climate change. Heat stress at the reproductive stage can result in pollen sterility and failure of grain development. This study used transcriptome data analysis to explore the specific expression of long non-coding RNAs (lncRNAs) in response to heat stress during pollen development in four wheat cultivars. We identified 11,054 lncRNA-producing loci, of which 5,482 lncRNAs showed differential expression in response to heat stress. Heat-responsive lncRNAs could target protein-coding genes in cis and trans and in lncRNA-miRNA-mRNA regulatory networks. Gene ontology analysis predicted that target protein-coding genes of lncRNAs regulate various biological processes such as hormonal responses, protein modification and folding, response to stress, and biosynthetic and metabolic processes. We also noted some paired lncRNA/protein-coding gene modules and some lncRNA-miRNA-mRNA regulatory modules shared in two or more wheat cultivars. These modules were related to regulating plant responses to heat stress, such as heat-shock proteins and transcription factors, and protein domains, such as MADS-box, Myc-type, and Alpha crystallin/Hsp20 domain. Our results provide the basic knowledge and molecular resources for future functional studies investigating wheat reproductive development under heat stress.

Glyphosate hormesis effects on the vegetative and reproductive development of glyphosate-susceptible and -resistant Conyza sumatrensis biotypes

Low glyphosate doses that produce hormesis may alter the susceptibility to herbicides of weeds or enhance their propagation and dispersal. The objective of this work was to evaluate the hormetic effects of glyphosate on the vegetative, phenological and reproductive development in resistant (R) and susceptible (S) Conyza sumatrensis biotypes. The glyphosate resistance level of biotype R was 11.2-fold compared to the S biotype. Glyphosate doses <11.25 g ae ha−1 induced temporary and permanent hormetic effects for the number of leaves, plant height and dry mass accumulation up to 28 d after application in both R and S biotypes. The S biotype required 15–19% fewer thermal units at 1.4 and 2.8 g ae ha−1 glyphosate than untreated plants to reach the bolting stage. Also, this biotype had less thermal units associated with the appearance (1225 vs 1408 units) and opening (1520 vs 1765 units) of the first capitulum than the R biotype. In addition, glyphosate affected reproductive traits of both biotypes compared to their controls, increasing the number of capitulum's and seeds per plant up to 37 and 41% (at 2.8 and 0.7 g ae h−1, respectively) in the S biotype, and by 48 and 114% (both at 5.6 g ae ha−1) in the R biotype. Depending on environmental parameters, glyphosate may or may not cause hormetic effects on the vegetative and phenological development of C. sumatrenis biotypes; however, this herbicide increases the speed and fecundity of reproduction, regardless of the glyphosate susceptibility level, which can alter the population dynamics and glyphosate susceptibility of future generations.

Isoliquiritin can cause mitochondrial dysfunction and regulate Nrf2 to affect the development of mouse oocytes

IsoliQuirtigenin (ILG) has been widely studied in somatic cells and tissues, but less in reproductive development. It is a kind of widely used food additive. In this study, it was found that ILG could significantly increase the levels of ROS,GSH and MMP in mouse oocytes (P < 0.01). In order to explore the cause of this phenomenon, it was found that the abnormal distribution of mitochondria and ATP synthesis levels were significantly increased (P < 0.05). At this time, we made a reasonable hypothesis that ILG affected mitochondrial function. In subsequent studies, it was found that the endogenous ROS accumulation level in mitochondria was significantly increased. After continuous RT-PCR screening, it was found that the expression of Nrf2 was significantly inhibited (P < 0.01). Its upstream and downstream FOXO3 GPX1, CAT, SOD2, SIRT1 gene also appear different degree of significant change (P < 0.05), in which the lower expression of NADP + (P < 0.05) illustrates the mitochondrial ATP synthesis electronic chain were suppressed, it also has the reason, By inhibiting electron chain and ATP synthesis, ILG leads to oocyte apoptosis and initiation of autophagy, reducing oocyte and its subsequent developmental potential.

Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development.

CircRNAs are a class of noncoding RNAs with tissue- and development-specific expression characteristics. In many mammals, primordial follicle development begins in the embryonic stage. However, the study of circRNAs in primordial follicle development in mice has not been reported. In this study, ovaries were collected from mouse foetuses at 15.5 days post coitus (dpc) and 17.5 dpc, which are two key stages of primordial follicle development. A total of 4785 circRNAs were obtained by using RNA-seq.Of these, 83 differentially expressed circRNAs were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these differential circRNAs were mainly involved in the regulation of reproductive development. Through qRT-PCR, back-splice sequence detection and enzyme digestion protection experiments, we found that circ-009346, circ-014674, circ-017054 and circ-008296 were indeed circular. Furthermore, circ-009346, circ-014674 and circ-017054 were identified as three key circRNAs by analysing their expression in the ovaries of mice at different developmental stages. The circRNA-miRNA-mRNA interaction network was constructed and validated for target miRNA and mRNA using qRT-PCR. The interacting genes circ-009346, circ-014674, and circ-017054 were subjected to KEGG enrichment analysis. We found that circ-014674 may participate in the assembly and reserve of primordial follicles through oestrogen and the Janus kinase (JAK) signal transducer and activator of transcription (STAT) signalling pathway (JAK-SATA). Circ-009346 and circ-017054 may have similar functions and are involved in the activation and growth of primordial follicles through the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) signalling pathways. Based on our findings, three circRNAs associated with primordial follicle development were identified, and their potential mechanisms of regulating primordial follicle development were revealed. These findings will help us better understand the molecular mechanism of circRNAs in primordial follicles and provide important references and targets for the development of primordial follicles.

Is reproductive development adaptively calibrated to early experience? Evidence from a national sample of females.

Many developmental theories have not been sufficiently evaluated using designs that control for unobserved familial confounds. Our long-term goal is to determine the causal structure underlying associations between early environmental conditions and later psychosocial and health outcomes. Our overall objective in this study was to further evaluate predictions derived from applications of life history theory to female reproductive development, key among them that reproductive milestones translate early environmental risk into fertility, health, and behavioral outcomes. To this end, we used female data from the National Longitudinal Survey of Youth 1979 and structural equation modeling to conduct increasingly severe tests, beginning with covariate control and then progressing to sibling control and behavioral genetic designs. After adjusting for confounds varying between sets of siblings, we did not find evidence that age at menarche reflected components of early environment or that any focal outcomes reflected early fragmented family structure (birth to age nine). Although we detected no links between measured environment and individual differences in age at sexual debut, we did find that it reflected both shared and nonshared influences in our behavior genetic models. Interestingly, delayed sexual debut (into young adulthood) reflected identification of parents as the greatest influences and forecasted an array of fertility-related outcomes. Taken together, these findings challenge theories suggesting menarche timing is adaptively calibrated to early environment. They also highlight the need for more research using sibling control and related designs to examine the roles of environments in development. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Toxic effect of elements on the germination and initial development of barley seeds (Hordeum vulgare L.)

Barley (Hordeum vulgare) is an agricultural vegetable from the Poaceae family used in food and beer production. The study aimed to evaluate the toxic effect of Aluminum (Al) and Copper (Cu) on germination and initial development in barley seeds cultivar KWS Irina. Different concentrations (0, 35, 85 and 125 mg L-1) of aqueous solution of Al and Cu were produced from their chlorides. The toxicity experiment was carried out in a germination box maintained in a germination chamber with a 12-h photoperiod. After 15 days of germination, the seedlings were measured using a millimetric ruler (cm) where they were evaluated for plant length, root length, and fresh and dry mass of plant and root determined on a digital analytical scale (g). Barley seedlings cultivar KWS Irina demonstrated to be intolerant to concentrations of the toxic elements Al and Cu in all plant parameters analyzed, except for plant dry mass. Future studies should be carried out comparing the initial and reproductive development of this barley cultivar in terms of the presence and absence of toxic elements.

Financial flows of restaurant business enterprises: essence, assessment of management efficiency

The purpose of the article is to study the essence of the concept of "financial flow" and improve the system of indicators for analyzing the effectiveness of the management of financial flows in the restaurant business enterprise. The study was conducted using a rigorous set of research methods, including theoretical generalization, scientific abstraction, systematization and decomposition, formalization, economic-mathematical, and graphic. These methods were chosen to ensure the robustness and reliability of the research findings. Research results. The article analyzes the main approaches to considering the essence of the concept of "financial flow" in modern scientific literature and defines its characteristic features. Features of managing the financial flows of restaurant business enterprises are determined. A model of the formation of the financial flow of a restaurant business enterprise has been developed. Mathematical models of the financial flows of a restaurant business enterprise are presented. For a justified managerial influence on the formation, distribution, and redistribution of financial resources, a model of the financial flow cycle of the restaurant business enterprise is proposed. To assess the sufficiency of financial flows for the implementation of current financial and economic activities and to optimize the time for making management decisions regarding current and operational financial management, a system of indicators for evaluating the effectiveness of financial flow management has been developed. This research has made a unique contribution to the field by further developing the system of indicators for evaluating the effectiveness of the management of financial flows of restaurant business enterprises. Unlike traditional approaches, this system allows for a comprehensive assessment of the sufficiency of the financial flow based on operational financial accounting data. This novel approach can help ensure the reproductive development of the restaurant business enterprise and optimize the time for making management decisions. The model of the formation of the financial flow of a restaurant business enterprise, which has been developed because of this research, has significant practical implications. It provides a means to control the rhythmicity of the operating cycle, contributes to the growth of activity volumes, forms economic relations between subjects, and aids in the formation of incoming and outgoing cash flows. These practical applications can potentially lead to improved financial management in the restaurant business sector. Practical significance. Implementing the developed risk management information system ensures a high level of validity of management decisions regarding strategic alternatives: risk rejection, risk diversification, or compensation. Further research is planned to analyze management and modern digital technologies (lean production, process mining, technological platforms, etc.) to increase the efficiency of the financial flow management process. Keywords: finance, financial flow, cash flow, financial management, enterprise, restaurant business, performance evaluation.

Highly resolved genome assembly and comparative transcriptome profiling reveal genes related to developmental stages of tapeworm Ligula intestinalis.

Ligula intestinalis (Cestoda: Diphyllobothriidae) is an emerging model organism for studies on parasite population biology and host-parasite interactions. However, a well-resolved genome and catalogue of its gene content has not been previously developed. Here, we present the first genome assembly of L. intestinalis, based on Oxford Nanopore Technologies, Illumina and Omni-C sequencing methodologies. We use transcriptome profiling to compare plerocercoid larvae and adult worms and identify differentially expressed genes (DEGs) associated with these life stages. The genome assembly is 775.3 mega (M)bp in size, with scaffold N50 value of 118 Mbp and encodes 27 256 predicted protein-coding sequences. Over 60% of the genome consists of repetitive sequences. Synteny analyses showed that the 10 largest scaffolds representing 75% of the genome display high correspondence to full chromosomes of cyclophyllidean tapeworms. Mapping RNA-seq data to the new reference genome, we identified 3922 differentially expressed genes in adults compared with plerocercoids. Gene ontology analyses revealed over-represented genes involved in reproductive development of the adult stage (e.g. sperm production) and significantly enriched DEGs associated with immune evasion of plerocercoids in their fish host. This study provides the first insights into the molecular biology of L. intestinalis and provides the most highly contiguous assembly to date of a diphyllobothriid tapeworm useful for population and comparative genomic investigations of parasitic flatworms.