Repression System Research Articles

Anti-assimilationist landscape: Becoming illegible as queer resistance to state power

Through a study of two queer contemporary photographers working with landscape imagery, the author proposes a theory of anti-assimilationist landscape: a politicized rejection of the conventions of landscape imagery and the repressive systems of land ownership, gender and nation-building it sustains. Collier Schorr and David Benjamin Sherry make landscape images in places with histories of gendered settler-colonial and white supremacist violence. The essay argues that their works function as both an interpretation of those sites and a queer reclamation of them, enacting a political ecology of difference that can allow for a politics outside of the liberal humanist notion of nationhood and the fascist notion of ‘blood and soil’. Arguing for its applicability to landscape architecture theory and practice, the author contrasts this anti-assimilationist mode with the turn-of-the-millennium concept of ‘eco-revelation’, proposing instead a queer aesthetics of obscuring and the potential of ‘becoming illegible’ as an environmentalist practice.

An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy.

Gene therapy has been adapted for improving malignant tumor treatment. However, pharmacotherapies targeting cancer remain limited and are generally inapplicable for rare disease patients. Oleanolic acid (OA) is a plant-derived triterpenoid that is frequently used in Chinese medicine as a safe but slow-acting treatment for many disorders. Here, the congruent pharmacological activities of OA and CRISPR-dCas9 in targeting AURKA or KDM1A and improving disease-specific prognosis and used a synthetic-biology-inspired design principle to engineer a therapeutic gene circuit that enables a concerted action of both drugs are utilized. In particular, the OA-triggered CRISPR-dCas9 transcriptional repression system rapidly and simultaneously attenuated lung and thyroid cancer. Collectively, this work shows that rationally engineered synthetic gene circuits are capable of treating multifactorial diseases in a synergistic manner by multiplexing the targeting efficiencies of single therapeutics.

Protocol for Delivery of CRISPR/dCas9 Systems for Epigenetic Editing into Solid Tumors Using Lipid Nanoparticles Encapsulating RNA.

Genome editing tools, particularly the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems (e.g., CRISPR/Cas9), and their repurposing into epigenetic editing platforms, offer enormous potential as safe and customizable therapies for cancer. Specifically, various transcriptional abnormalities in human malignancies, such as silencing of tumor suppressors and ectopic re-expression of oncogenes, have been successfully targeted with virtually no off-target effects using CRISPR activation and repression systems. In these systems, the nuclease-deactivated Cas9 protein (dCas9) is fused to one or more domains inducing selective activation or repression of the targeted genes. Despite these advances, the efficient in vivo delivery of these molecules into the target cancer cells represents a critical barrier to accomplishing translation into a clinical therapy setting for cancer. Major obstacles include the large size of dCas9 fusion proteins, the necessity of multimodal delivery of protein and gRNAs, and the potential of these formulations to elicit detrimental immune responses.In this context, viral methods for delivering CRISPR face several limitations, such as the packaging capacity of the viral genome, the potential for integration of the nucleic acids into the host cells genome, and immunogenicity of viral proteins, posing serious safety concerns. The rapid development of mRNA vaccines in response to the COVID-19 pandemic has rekindled interest in mRNA-based approaches for CRISPR/dCas9 delivery. Simultaneously, due to their high loading capacity, scalability, customizable surface modification for cell targeting, and low immunogenicity, lipid nanoparticles (LNPs) have been widely explored as nonviral vectors. In this chapter, we first describe the design of optimized dCas9-effector mRNAs and gRNAs for epigenetic editing. We outline formulations of LNPs suitable for dCas9 mRNA delivery. Additionally, we provide a protocol for the co-encapsulation of the dCas9-effector mRNAs and gRNA into these LNPs, along with detailed methods for delivering these formulations to both cell lines (in vitro) and mouse models of breast cancer (in vivo).

Ratni zločini bugarskih okupacionih trupa u Poljanici 1942‒1943. godine

After the April collapse of the army of the Kingdom of Yugoslavia in 1941, Bulgarian troops established control over certain parts of the territory of southern Serbia. In the occupied territory of Yugoslavia, Bulgaria established an occupation administration and established a repressive administrative system, the ultimate goal of which was the Bulgarianization of public life and the Serbian population, and the complete integration of the occupied territories into the state-legal order of Bulgaria. Guided by the stated objectives, from April 1941 to September 1944, the Bulgarian occupation troops committed a large number of war crimes against the Serbian population. The subject of our research is the crimes of the occupation forces in the Poljanica region during 1943. Using unpublished sources from the Bulgarian province, the paper provides hitherto unknown information about the suffering of Poljanica in the Second World War.

Whose Hand Writes the Story of ‘Us’? Vulnerability to Identity Interpellations in a Nonrepressive Social Context

I propose a reconsideration of the role that ‘interpellations’ play in the dynamic process of identity construction. ‘Interpellation’ is a quasi-technical term introduced by Louis Althusser (1969) that I reinterpret using the lens of contemporary social ontology. I therefore look at it as an identity proposal that the individual can either accept, reject, negotiate, or outright ignore. In the original text, Althusser mentions the fact that the individual’s acceptance is the essential moment of an interpellation, but he does not elaborate on the other options available to the individual. I believe it is important to analyze the individual’s options and the factors that may influence whether the individual’s freedom of choice is fully exercised in such contexts. I further discuss the human vulnerabilities that cause ‘interpellations’ to be adopted without much reflection, even in the absence of a repressive system that would try to enforce a mainstream ideology.

НИЩЕННЯ ГОСПОДАРЯ – СКЛАДОВА ЗЛОЧИНУ ГОЛОДОМОРУ-ГЕНОЦИДУ 1932–1933 РОКІВ

The article reveals the destruction by the Soviet command-repressive system of the economic freedoms of the Ukrainian rural producer, the prosperous peasant (kulak), a middle-class representative in the village – the guarantor of the socio-political and economic stability of the state. Since the late 1920s, a command-repressive system of governance was formed in the Ukrainian village, implemented by the Soviet communist political regime. In the context of the problem, it is important to study the dynamics of the destruction of prosperous producers (dekulakisation), which is a component of the criminal genocidal policy of the Soviet totalitarian regime against the Ukrainian nation. During 1918–1920, the Bolsheviks’ periodic invasions of Ukraine were marked by the introduction of a policy of «war communism», when «military communist» methods ensured the non-fixed collection of taxes. In March 1921, the Soviet political regime radically changed the taxation system in the village. Instead of the pre-tax system, a fixed food tax was implemented, and the New Economic Policy was introduced. The years 1928–1929 proved to be a crucial turning point in the relations between the peasant producer and the Soviet state. Since the late 1920s, an overall offensive by the Soviet political regime against the Ukrainian village affected several important segments for the village: the church, economic and political repression of the prosperous producers, forced grain procurement, and forced collectivisation. In 1929–1931, the article examines the example of the village of Zaruddia in Poltava region to demonstrate the formation of a command and repressive system of governance. In the late 1920s and early 1930s, the destruction of the spiritual, ethical, socio-economic foundations of the traditional Ukrainian peasant by the Soviet political regime is studied on a specific historical example. The Soviet political communist regime, forming a command-repressive system of governance since the late 1920s, violated human and civil rights and humiliated human dignity. Such actions caused irreparable spiritual, ethical, socio-economic damage in the traditional Ukrainian village.

A robust CRISPR interference gene repression system in Vibrio parahaemolyticus

Vibrio parahaemolyticus is a significant cause of seafood-associated gastroenteritis and pestilence in aquaculture worldwide. Despite extensive research, strategies for protein depletion in this pathogen remain limited. Herein, we constructed a new CRISPR interference (CRISPRi) system for gene repression based on the combination of a shuttle vector pVv3 and the nuclease-null Cas9 variant (dead Cas9, or dCas9) from Streptococcus pyrogens. This CRISPRi is induced by adding both IPTG and arabinose. We showed that gene repression is scalable via the use of multiple sgRNAs. We also demonstrated that this gene repression can be precisely tuned by adjusting the amount of two different inducers and can be reversed by removing the inducers. This system provides a simple approach for selective gene repression on a genome-wide scale in V. parahaemolyticus. Application of this system will dramatically accelerate investigations of this bacterium, including studies of physiology, pathogenesis, and drug target discovery.

Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae.

Clustered regularly interspaced short palindromic repeats (CRISPR) are prokaryotic adaptive immune systems regularly utilized as DNA-editing tools. While Neisseria gonorrhoeae does not have an endogenous CRISPR, the commensal species Neisseria lactamica encodes a functional Type I-C CRISPR-Cas system. We have established an isopropyl β-d-1-thiogalactopyranoside added (IPTG)-inducible, CRISPR interference (CRISPRi) platform based on the N. lactamica Type I-C CRISPR missing the Cas3 nuclease to allow locus-specific transcriptional repression. As proof of principle, we targeted a non-phase-variable version of the opaD gene. We show that CRISPRi can downregulate opaD gene and protein expression, resulting in bacterial inability to stimulate neutrophil oxidative responses and to bind to an N-terminal fragment of CEACAM1. Importantly, we used CRISPRi to effectively knockdown all the transcripts of all 11 opa genes using a five-spacer CRISPR array, allowing control of the entire phase-variable opa family in strain FA1090. We also report that repression is reversible following IPTG removal. Finally, we showed that the Type I-C CRISPRi system can conditionally reduce the expression of two essential genes. This CRISPRi system will allow the interrogation of every Gc gene, essential and non-essential, to study physiology and pathogenesis and aid in antimicrobial development.IMPORTANCEClustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have proven instrumental in genetically manipulating many eukaryotic and prokaryotic organisms. Despite its usefulness, a CRISPR system had yet to be developed for use in Neisseria gonorrhoeae (Gc), a bacterium that is the main etiological agent of gonorrhea infection. Here, we developed a programmable and IPTG-inducible Type I-C CRISPR interference (CRISPRi) system derived from the commensal species Neisseria lactamica as a gene repression system in Gc. As opposed to generating genetic knockouts, the Type I-C CRISPRi system allows us to block transcription of specific genes without generating deletions in the DNA. We explored the properties of this system and found that a minimal spacer array is sufficient for gene repression while also facilitating efficient spacer reprogramming. Importantly, we also show that we can use CRISPRi to knockdown genes that are essential to Gc that cannot normally be knocked out under laboratory settings. Gc encodes ~800 essential genes, many of which have no predicted function. We predict that this Type I-C CRISPRi system can be used to help categorize gene functions and perhaps contribute to the development of novel therapeutics for gonorrhea.

Characterization of a unique repression system present in arbitrium phages of the SPbeta family

Arbitrium-coding phages use peptides to communicate and coordinate the decision between lysis and lysogeny. However, the mechanism by which these phages establish lysogeny remains unknown. Here, focusing on the SPbeta phage family's model phages phi3T and SPβ, we report that a six-gene operon called the "SPbeta phages repressor operon" (sro) expresses not one but two master repressors, SroE and SroF, the latter of which folds like a classical phage integrase. To promote lysogeny, these repressors bind to multiple sites in the phage genome. SroD serves as an auxiliary repressor that, with SroEF, forms the repression module necessary for lysogeny establishment and maintenance. Additionally, the proteins SroABC within the operon are proposed to constitute the transducer module, connecting the arbitrium communication system to the activity of the repression module. Overall, this research sheds light on the intricate and specialized repression system employed by arbitrium SPβ-like phages in making lysis-lysogeny decisions.

Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system

Visualisation of genomic loci by microscopy is essential for understanding nuclear organisation, particularly at the single cell level. One powerful technique for studying the positioning of genomic loci is through the Lac Operator-Lac Repressor (LacO-LacI) system, in which LacO repeats introduced into a specific genomic locus can be visualised through expression of a LacI-protein fused to a fluorescent tag. First utilised in Trypanosoma brucei over 20 years ago, we have now optimised this system with short, stabilised LacO repeats of less than 2 kb paired with a constitutively expressed mNeongreen::LacI fusion protein to facilitate visualisation of genomic loci. We demonstrate the compatibility of this system with super-resolution microscopy and propose its suitability for multiplexing with inducible RNAi or protein over expression which will allow analysis of nuclear organisation after perturbation of gene expression.

Capturing Contemporary India (Fine Balance) By Rohinton Mistry

This is the most somber and latest novel. In A Fine Balance India once again becomes the central source of concern. The Narration takes us to the mid seventies when the Prime Minister imposes Emergency without any consultation of the Cabinet. The story revolves around the lives of four protagonists each very different from the next. They find themselves thrown together in the same humble city apartment: Dinabhai, a widow who refuses to remarry and fights to earn a meager living as a seamstress; two tailors, Ishvar and Ompraksh, uncle and nephew, who comes to the city in the hope of finding work; and a student Maneck Kohlah, from a village situated at the foothills of the Himalayas. Maneck's father has sent his son to a city school. Primarily, Mistry manages to bring forth the horror and devastation wreaked by the Emergency in all its vivid portrayal. The novel is considered to be a vehement commentary on the political and social environment of the times and the beautiful tragedy of emergence. The novel begins in 1975 with the accidental meeting of Ishvar, Ompraksh and Maneck Kohlah in a train. The first chapter recounts the story of Dina balal from the age of twelve and the death of her father to the age of forty-two and the hiring of the tailors. The second chapter introduces us to the manager of Au Revoir Exports, Mrs. Gupts. It is through the approval of Mrs. Gandhi's action we are confronted with the complicity of the Indian business houses. In the third chapter we are again taken to the times of Ishvar and Omprakash's story. The starkest depiction of Caste oppression is in this chapter. The fifth section deals with the story of Maneck. His story carries the ecological denudation of the Himalayas and the death of indigenous enterprises. In its particular theme the novel is about Mrs. Dina Dalai, who becomes widow three years after marriage, resolves to remain financially independent takes two Hindu Tailors as boarders to sew dresses for an export company. The two tailors are Ishavar and his nephew Om, who left their village in an effort to escape the repressive Caste system. Maneck, who is the son of the old school friend of Dina is sent to attend the college as the family falls in business. The crux and central concerns of the novel revolves around the dialectical and dialogical interactions of these characters. The dreams, ambitions, the trails, failures and success of these characters in the face of the oppressing order of the society. For four months a family is evolved in the social, emotional interactions of these characters. Eating and sleeping together, sharing the dreams, meals and the space, the characters transcend the barriers of Caste, religion and economic status. The cramped and conjusted space becomes a haven from the political and social turmoils of the society. These four characters experience unpleasant encounters and are repeatedly saved from the quaint character, the Beggarmaster. All this narration of the novel is at the backdrop of the emergency period imposed by Indira Gandhi's rule.

How the first revolution affected the second: The setback of 1927 for the Chinese Communist Party Revolution in the 1920s

AbstractThe Bolsheviks’ world revolution encountered setbacks in the 1920s. Among the bloodiest of these was the massacre of 1927 when the Chinese Communist Party’s (CCP) entire central leadership was killed in the Chinese Nationalist Party’s (GMD) coup. Existing explanations highlight Moscow’s miscalculation, infighting within the Kremlin, Soviet advisers’ information dilemma, and the CCP leaders’ political inexperience. This article compares the opening stages of the Bolshevik (or Russian) and Chinese Communist Party revolutions to explain why the 1927 setback became a catastrophe. It argues that the Bolshevik Revolution of 1917 caused fundamental changes, which thwarted any attempt to replicate the 1917 victory in the post-1917 world. The CCP in 1927 faced three disadvantages that the Bolshevik Revolution had engendered: a misleading myth about the October Revolution, a Bolshevized system of repression created by Soviet advisers to the GMD, and the ‘red scare’ in Japan and British Southeast Asia, which blocked members of the CCP from escaping overseas. This article draws on leaders’ biographical materials to compare the two parties’ learning from foreign revolutions, records in suffering repression, and experiences as overseas refugees. The comparison shows that the Bolsheviks did not face these three disadvantages before 1917.

Metabolic Engineering of Pichia pastoris for High-Level Production of Lycopene.

Lycopene is widely used in cosmetics, food, and nutritional supplements. Microbial production of lycopene has been intensively studied. However, few metabolic engineering studies on Pichia pastoris have been aimed at achieving high-yield lycopene production. In this study, the CRISPR/Cpf1-based gene repression system was developed and the gene editing system was optimized, which were applied to improve lycopene production successfully. In addition, the sterol regulatory element-binding protein SREBP (Sre) was used for the regulation of lipid metabolic pathways to promote lycopene overproduction in P. pastoris for the first time. The final engineered strain produced lycopene at 7.24 g/L and 75.48 mg/g DCW in fed-batch fermentation, representing the highest lycopene yield in P. pastoris reported to date. These findings provide effective strategies for extended metabolic engineering assisted by the CRISPR/Cpf1 system and new insights into metabolic engineering through transcriptional regulation of related metabolic pathways to enhance carotenoid production in P. pastoris.

Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit.

A proper balance of metabolic pathways is crucial for engineering microbial strains that can efficiently produce biochemicals on an industrial scale while maintaining cell fitness. High production loads can negatively impact cell fitness and hinder industrial-scale production. To address this, fine-tuning gene expression using engineered promoters and genetic circuits can promote control over multiple targets in pathways and reduce the burden. We took advantage of the robust carbon catabolite repression system of Bacillus subtilis to engineer a glucose-inducible genetic circuit that supports growth and production. The circuit is resilient, enabling a quick switch in the production status when exposed to the correct carbon source. By performing serial cultivations for 61 generations under repressive conditions, we preserved the production capacity of the cells, which could be fully accessed by switching to glucose in the next cultivation step. Switching to glucose after 61 generations resulted in 34-fold activation and generated 70% higher production in comparison to standard cultivation in glucose. Conversely, serial cultivation under permanent induction resulted in 62% production loss after 67 generations alongside an increase in the culture growth rate. As a pathway-independent circuit activated by the preferred carbon source, our engineered glucose-inducible genetic circuit is broadly useful and imposes no additional cost to traditional production processes.

Undermining the ‘Polder Model’: Workers’ Militancy and Trade-Union Leadership in Four Dutch Wildcat Strikes, 1963–1970

All over Europe, workers’ insubordination in the 1960s and 1970s became manifest in a growing number of wildcat strikes. In the Netherlands, under the guise of the so-called consensual ‘polder model’, trade unions were part of a repressive system of industrial relations. From 1963, rank-and-file opposition in ‘unofficial’ strikes forced the ‘official’ unions to abandon their support of restrictive wage policies. After a huge wildcat strike in the port of Rotterdam in 1970, the union leadership changed its policy and began to initiate strikes. In this article, rank-and-file mobilization and the union leaderships’ reaction are analysed in four wildcat strikes between 1963 and 1970. It concludes that in these strikes the union leadership and rank-and-file members, while opposed to each other, were also mutually dependent. The union leadership was forced to adapt to pressure from below, and strikers had to rely on the bargaining ability of union officials to reach an agreement with the employers. Both parties had to accept an involuntary symbiosis. In this way, the attachment of the trade-union leadership to the top-down consensus in the ‘polder model’ was undermined.

A SULAPA EPPE’E MODEL CONSTRUCTION FOR THE LEGAL PROTECTION OF THE LIFE ENVIRONMENT IN THE COASTAL FISHING RESOURCE EXPLOITATION AT SOUTH SULAWESI PROVINCE

This research analyzes the construction model of Sulapa Eppe'e in environmental law protection for the utilization of coastal fishery resources. This type of research uses a normative-empirical constructivism paradigm. The results of the study show that Sulapa Eppe'e adheres to a four-dimensional system of legal relations that places humans as the center in relations with God, nature (environment), society, and the government and the state. Model construction includes: concepts, philosophies, paradigms, ideologies, basic values, norms, principles, analytical methods and practices. This model gave birth to three pillars of wisdom, namely local wisdom, ecological wisdom, and the legal wisdom of the Mampanre'tasi tradition (conservation and zoning system), Rompong (protection and management system), Pemali and Palili'(prevention and repressive system), Mamia Kadialo (ecological wisdom values), Ponggawa – sawi (coordination and cooperation system). The Sulapa Eppe'e model can play an important role in overcoming tensions, integrating and harmonizing political products, ecological products and cultural products from environmental law.

Polarisasi Politik di Indonesia 2014-2019: Sebuah Kajian Pustaka

In the 1950s, political life was divided due to sect politics. The New Order and Reform had suppressed and made political polarization subside, but political polarization strengthened again in 2014, due to the meeting of two spectrums between pluralist and Islamist groups. This study aims to examine the causes of the strengthening of political polarization between Islamists and pluralists in Indonesia, after it has faded for so long in a repressive and open political system. This research uses a qualitative approach with literature study method. The results of the study found that political actors pursue their political goals by using polarization strategies, such as mobilizing voters to divide them, spreading hate speech, and exploiting public unrest. The conclusion is that the political polarization that occurred between Islamists and pluralists in Indonesia in 2014-2019 was caused by strategic political actors. This research will add to the study of political polarization in Indonesia which is still limited, especially those discussing the causes of political polarization.

Crossing bacterial boundaries: The carbon catabolite repression system Crc-Hfq of Pseudomonas putida KT2440 as a tool to control translation in E. coli

As a global regulatory mechanism, carbon catabolite repression allows bacteria and eukaryal microbes to preferentially utilize certain substrates from a mixture of carbon sources. The mechanism varies among different species. In Pseudomonas spp., it is mainly mediated by the Crc-Hfq complex which binds to the 5′ region of the target mRNAs, thereby inhibiting their translation. This molecular mechanism enables P. putida to rapidly adjust and fine-tune gene expression in changing environments. Hfq is an RNA-binding protein that is ubiquitous and highly conserved in bacterial species. Considering the characteristics of Hfq, and the widespread use and rapid response of Crc-Hfq in P. putida, this complex has the potential to become a general toolbox for post-transcriptional multiplex regulation. In this study, we demonstrate for the first time that transplanting the pseudomonal catabolite repression protein, Crc, into E. coli causes multiplex gene repression. Under the control of Crc, the production of a diester and its precursors was significantly reduced. The effects of Crc introduction on cell growth in both minimal and rich media were evaluated. Two potential factors - off-target effects and Hfq-sequestration - could explain negative effects on cell growth. Simultaneous reduction of off-targeting and increased sequestration of Hfq by the introduction of the small RNA CrcZ, indicated that Hfq sequestration plays a more prominent role in the negative side-effects. This suggests that the negative growth effect can be mitigated by well-controlled expression of Hfq. This study reveals the feasibility of controlling gene expression using heterologous regulation systems.

Criticism of Social, Political, and Religious Problems in Indonesia: A Study on Al-Iklīl fī Ma‘ānī al-Tanzīl by Misbah Bin Zainil Mustafa (1917-1994)

Criticism of Social, Political, and Religious Problems in Indonesia: A Study on Al-Iklīl fī Ma‘ānī al-Tanzīl by Misbah Bin Zainil Mustafa (1917-1994)

Beyond mass graves: exhuming Francoist concentration camps

As several historical investigations have revealed, between 130,000 and 150,000 Republicans were executed during the Spanish Civil War (1936–1939) and Franco’s dictatorship (1939–1977). The Francoist repressive strategy – unleashed after the coup d’état of 17 July 1936 – developed complex mechanisms of physical and psychological punishment. The continuing subjugation of those still living was enacted through concentration camps, prisons and forced labour. During the War and Franco’s dictatorship, there were nearly three hundred concentration camps, and between 367,000 and 500,000 prisoners went through those camps. During the transition to democracy, neither the State nor the judiciary investigated mass crimes connected to the repression and execution of left-wing Republicans. After Franco’s death, some family groups recovered some of these bodies buried in unmarked mass graves without scientific involvement. In the year 2000, the first scientific exhumations took place, and since then, more than 400 mass graves have been opened, and up to 9.000 bodies have been recovered. The memory of the victims of Franco’s violence has been mainly centralised on mass graves. The opening of mass graves has positioned the Spanish Civil War case within the international sphere of human rights violations and has also opened a new window of opportunity for the analysis of Francoist concentration camps. In this article, I provide a holistic study of mass graves that combines archaeology and forensic anthropology with historical and ethnographic research in order to examine, in detail, both the burials and the broader landscape of the repression. In this contribution, I focus on the Concentration Camp of Castuera, in southwestern Spain, a forgotten campscape, and show how mass graves, which have become widely known as sites of research and commemoration in Spain, were closely related to the camps’ complex repressive system. My results have allowed me to conduct an integrated analysis of this context of political violence. I conclude that archaeology and forensic anthropology have played a crucial role in elucidating the functioning and social reality of Spanish camps, whilst enabling new narratives about past Francoist repression.